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GNB3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol GNB3
Entrez Gene ID 2784
Full Name guanine nucleotide binding protein (G protein), beta polypeptide 3
Synonyms
General protein information
Preferred Names
guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3
Names
guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3
transducin beta chain 3
G protein, beta-3 subunit
hypertension associated protein
GTP-binding regulatory protein beta-3 chain
guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12p13

Summary Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit which belongs to the WD repeat G protein beta family. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. A single-nucleotide polymorphism (C825T) in this gene is associated with essential hypertension and obesity. This polymorphism is also associated with the occurrence of the splice variant GNB3-s, which appears to have increased activity. GNB3-s is an example of alternative splicing caused by a nucleotide change outside of the splice donor and acceptor sites. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]. lac of sum
Disorder MIM:

139130

Disorder Html: {Hypertension, essential, susceptibility to}, 145500 (3)





The following GNB3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNB3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu58008 XM_011520953 PREDICTED: Homo sapiens guanine nucleotide binding protein (G protein), beta polypeptide 3 (GNB3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu58009 XM_011520954 PREDICTED: Homo sapiens guanine nucleotide binding protein (G protein), beta polypeptide 3 (GNB3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu28823 NM_002075 Homo sapiens guanine nucleotide binding protein (G protein), beta polypeptide 3 (GNB3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu28836 NM_001297571 Homo sapiens guanine nucleotide binding protein (G protein), beta polypeptide 3 (GNB3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu58008
Accession Version XM_011520953.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1083bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009759.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGGGGAGA TGGAGCAACT GCGTCAGGAA GCGGAGCAGC TCAAGAAGCA GATTGCAGAT 
GCCAGGAAAG CCTGTGCTGA CGTTACTCTG GCAGAGCTGG TGTCTGGCCT AGAGGTGGTG
GGACGAGTCC AGATGCGGAC GCGGCGGACG TTAAGGGGAC ACCTGGCCAA GATTTACGCC
ATGCACTGGG CCACTGATTC TAAGCTGCTG GTAAGTGCCT CGCAAGATGG GAAGCTGATC
GTGTGGGACA GCTACACCAC CAACAAGGTG CACGCCATCC CACTGCGCTC CTCCTGGGTC
ATGACCTGTG CCTATGCCCC ATCAGGGAAC TTTGTGGCAT GTGGGGGGCT GGACAACATG
TGTTCCATCT ACAACCTCAA ATCCCGTGAG GGCAATGTCA AGGTCAGCCG GGAGCTTTCT
GCTCACACAG GTTATCTCTC CTGCTGCCGC TTCCTGGATG ACAACAATAT TGTGACCAGC
TCGGGGGACA CCACGTGTGC CTTGTGGGAC ATTGAGACTG GGCAGCAGAA GACTGTATTT
GTGGGACACA CGGGTGACTG CATGAGCCTG GCTGTGTCTC CTGACTTCAA TCTCTTCATT
TCGGGGGCCT GTGATGCCAG TGCCAAGCTC TGGGATGTGC GAGAGGGGAC CTGCCGTCAG
ACTTTCACTG GCCACGAGTC GGACATCAAC GCCATCTGTT TCTTCCCCAA TGGAGAGGCC
ATCTGCACGG GCTCGGATGA CGCTTCCTGC CGCTTGTTTG ACCTGCGGGC AGACCAGGAG
CTGATCTGCT TCTCCCACGA GAGCATCATC TGCGGCATCA CGTCCGTGGC CTTCTCCCTC
AGTGGCCGCC TACTATTCGC TGGCTACGAC GACTTCAACT GCAATGTCTG GGACTCCATG
AAGTCTGAGC GTGTGGGTAA GGGCCAGCCC TGGCTGCTGC TTCCTCAGCT GGAAGGACCC
TCCCCAGCCC TCCCTCCCCA TTCTGTACCC CCCATCAGCT CCCATTTCGG ACTCTCTTAC
TGCTGTCCCT TGTCACTGGG TGACTCCACC CCTGGAATCC AGTACCCCTT GGTTCCCAAC
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011519255.1
CDS143..1225
Misc Feature(1)284..1063(+)
Misc Feature(2)287..1063(+)
Misc Feature(3)302..1036(+)
Translation
Position Chain Variation Link
18 18 a, t dbSNP:751757865
170 170 a, g dbSNP:754210939
175 175 a, g dbSNP:566098814
601 601 c, t dbSNP:200864177
762 762 a, c, g dbSNP:200634640
852 852 a, g dbSNP:113437937
868 868 c, g dbSNP:760856626
877 877 a, g dbSNP:112732503
883 883 c, t dbSNP:13306410
956 956 a, g dbSNP:5442
967 967 c, t dbSNP:5443
1012 1012 c, t dbSNP:753136154
1022 1022 a, t dbSNP:200733665
1079 1079 c, t dbSNP:780628128

Target ORF information:

RefSeq Version XM_011520953
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens guanine nucleotide binding protein (G protein), beta polypeptide 3 (GNB3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011520953

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGGGGAGA TGGAGCAACT GCGTCAGGAA GCGGAGCAGC TCAAGAAGCA GATTGCAGAT 
GCCAGGAAAG CCTGTGCTGA CGTTACTCTG GCAGAGCTGG TGTCTGGCCT AGAGGTGGTG
GGACGAGTCC AGATGCGGAC GCGGCGGACG TTAAGGGGAC ACCTGGCCAA GATTTACGCC
ATGCACTGGG CCACTGATTC TAAGCTGCTG GTAAGTGCCT CGCAAGATGG GAAGCTGATC
GTGTGGGACA GCTACACCAC CAACAAGGTG CACGCCATCC CACTGCGCTC CTCCTGGGTC
ATGACCTGTG CCTATGCCCC ATCAGGGAAC TTTGTGGCAT GTGGGGGGCT GGACAACATG
TGTTCCATCT ACAACCTCAA ATCCCGTGAG GGCAATGTCA AGGTCAGCCG GGAGCTTTCT
GCTCACACAG GTTATCTCTC CTGCTGCCGC TTCCTGGATG ACAACAATAT TGTGACCAGC
TCGGGGGACA CCACGTGTGC CTTGTGGGAC ATTGAGACTG GGCAGCAGAA GACTGTATTT
GTGGGACACA CGGGTGACTG CATGAGCCTG GCTGTGTCTC CTGACTTCAA TCTCTTCATT
TCGGGGGCCT GTGATGCCAG TGCCAAGCTC TGGGATGTGC GAGAGGGGAC CTGCCGTCAG
ACTTTCACTG GCCACGAGTC GGACATCAAC GCCATCTGTT TCTTCCCCAA TGGAGAGGCC
ATCTGCACGG GCTCGGATGA CGCTTCCTGC CGCTTGTTTG ACCTGCGGGC AGACCAGGAG
CTGATCTGCT TCTCCCACGA GAGCATCATC TGCGGCATCA CGTCCGTGGC CTTCTCCCTC
AGTGGCCGCC TACTATTCGC TGGCTACGAC GACTTCAACT GCAATGTCTG GGACTCCATG
AAGTCTGAGC GTGTGGGTAA GGGCCAGCCC TGGCTGCTGC TTCCTCAGCT GGAAGGACCC
TCCCCAGCCC TCCCTCCCCA TTCTGTACCC CCCATCAGCT CCCATTTCGG ACTCTCTTAC
TGCTGTCCCT TGTCACTGGG TGACTCCACC CCTGGAATCC AGTACCCCTT GGTTCCCAAC
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58009
Accession Version XM_011520954.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1080bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009759.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGGGGAGA TGGAGCAACT GCGTCAGGAA GCGGAGCAGC TCAAGAAGCA GATTGCAGAT 
GCCAGGAAAG CCTGTGCTGA CGTTACTCTG GCAGAGCTGG TGTCTGGCCT AGAGGTGGTG
GGACGAGTCC AGATGCGGAC GCGGCGGACG TTAAGGGGAC ACCTGGCCAA GATTTACGCC
ATGCACTGGG CCACTGATTC TAAGCTGCTG GTAAGTGCCT CGCAAGATGG GAAGCTGATC
GTGTGGGACA GCTACACCAC CAACAAGGTG CACGCCATCC CACTGCGCTC CTCCTGGGTC
ATGACCTGTG CCTATGCCCC ATCAGGGAAC TTTGTGGCAT GTGGGGGGCT GGACAACATG
TGTTCCATCT ACAACCTCAA ATCCCGTGAG GGCAATGTCA AGGTCAGCCG GGAGCTTTCT
GCTCACACAG GTTATCTCTC CTGCTGCCGC TTCCTGGATG ACAACAATAT TGTGACCAGC
TCGGGGGACA CCACTGCCTT GTGGGACATT GAGACTGGGC AGCAGAAGAC TGTATTTGTG
GGACACACGG GTGACTGCAT GAGCCTGGCT GTGTCTCCTG ACTTCAATCT CTTCATTTCG
GGGGCCTGTG ATGCCAGTGC CAAGCTCTGG GATGTGCGAG AGGGGACCTG CCGTCAGACT
TTCACTGGCC ACGAGTCGGA CATCAACGCC ATCTGTTTCT TCCCCAATGG AGAGGCCATC
TGCACGGGCT CGGATGACGC TTCCTGCCGC TTGTTTGACC TGCGGGCAGA CCAGGAGCTG
ATCTGCTTCT CCCACGAGAG CATCATCTGC GGCATCACGT CCGTGGCCTT CTCCCTCAGT
GGCCGCCTAC TATTCGCTGG CTACGACGAC TTCAACTGCA ATGTCTGGGA CTCCATGAAG
TCTGAGCGTG TGGGTAAGGG CCAGCCCTGG CTGCTGCTTC CTCAGCTGGA AGGACCCTCC
CCAGCCCTCC CTCCCCATTC TGTACCCCCC ATCAGCTCCC ATTTCGGACT CTCTTACTGC
TGTCCCTTGT CACTGGGTGA CTCCACCCCT GGAATCCAGT ACCCCTTGGT TCCCAACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011519256.1
CDS143..1222
Misc Feature(1)284..1060(+)
Misc Feature(2)287..1060(+)
Misc Feature(3)302..1033(+)
Translation
Position Chain Variation Link
18 18 a, t dbSNP:751757865
170 170 a, g dbSNP:754210939
175 175 a, g dbSNP:566098814
601 601 c, t dbSNP:200864177
759 759 a, c, g dbSNP:200634640
849 849 a, g dbSNP:113437937
865 865 c, g dbSNP:760856626
874 874 a, g dbSNP:112732503
880 880 c, t dbSNP:13306410
953 953 a, g dbSNP:5442
964 964 c, t dbSNP:5443
1009 1009 c, t dbSNP:753136154
1019 1019 a, t dbSNP:200733665
1076 1076 c, t dbSNP:780628128

Target ORF information:

RefSeq Version XM_011520954
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens guanine nucleotide binding protein (G protein), beta polypeptide 3 (GNB3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011520954

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGGGGAGA TGGAGCAACT GCGTCAGGAA GCGGAGCAGC TCAAGAAGCA GATTGCAGAT 
GCCAGGAAAG CCTGTGCTGA CGTTACTCTG GCAGAGCTGG TGTCTGGCCT AGAGGTGGTG
GGACGAGTCC AGATGCGGAC GCGGCGGACG TTAAGGGGAC ACCTGGCCAA GATTTACGCC
ATGCACTGGG CCACTGATTC TAAGCTGCTG GTAAGTGCCT CGCAAGATGG GAAGCTGATC
GTGTGGGACA GCTACACCAC CAACAAGGTG CACGCCATCC CACTGCGCTC CTCCTGGGTC
ATGACCTGTG CCTATGCCCC ATCAGGGAAC TTTGTGGCAT GTGGGGGGCT GGACAACATG
TGTTCCATCT ACAACCTCAA ATCCCGTGAG GGCAATGTCA AGGTCAGCCG GGAGCTTTCT
GCTCACACAG GTTATCTCTC CTGCTGCCGC TTCCTGGATG ACAACAATAT TGTGACCAGC
TCGGGGGACA CCACTGCCTT GTGGGACATT GAGACTGGGC AGCAGAAGAC TGTATTTGTG
GGACACACGG GTGACTGCAT GAGCCTGGCT GTGTCTCCTG ACTTCAATCT CTTCATTTCG
GGGGCCTGTG ATGCCAGTGC CAAGCTCTGG GATGTGCGAG AGGGGACCTG CCGTCAGACT
TTCACTGGCC ACGAGTCGGA CATCAACGCC ATCTGTTTCT TCCCCAATGG AGAGGCCATC
TGCACGGGCT CGGATGACGC TTCCTGCCGC TTGTTTGACC TGCGGGCAGA CCAGGAGCTG
ATCTGCTTCT CCCACGAGAG CATCATCTGC GGCATCACGT CCGTGGCCTT CTCCCTCAGT
GGCCGCCTAC TATTCGCTGG CTACGACGAC TTCAACTGCA ATGTCTGGGA CTCCATGAAG
TCTGAGCGTG TGGGTAAGGG CCAGCCCTGG CTGCTGCTTC CTCAGCTGGA AGGACCCTCC
CCAGCCCTCC CTCCCCATTC TGTACCCCCC ATCAGCTCCC ATTTCGGACT CTCTTACTGC
TGTCCCTTGT CACTGGGTGA CTCCACCCCT GGAATCCAGT ACCCCTTGGT TCCCAACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28823
Accession Version NM_002075.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1023bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU143349.1, BC002454.2, U47924.1 and BC015920.1. This sequence is a reference standard in the RefSeqGene project. On Jul 3, 2014 this sequence version replaced gi:21071080. Summary: Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit which belongs to the WD repeat G protein beta family. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. A single-nucleotide polymorphism (C825T) in this gene is associated with essential hypertension and obesity. This polymorphism is also associated with the occurrence of the splice variant GNB3-s, which appears to have increased activity. GNB3-s is an example of alternative splicing caused by a nucleotide change outside of the splice donor and acceptor sites. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC002454.2, AB593098.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGGGGAGA TGGAGCAACT GCGTCAGGAA GCGGAGCAGC TCAAGAAGCA GATTGCAGAT 
GCCAGGAAAG CCTGTGCTGA CGTTACTCTG GCAGAGCTGG TGTCTGGCCT AGAGGTGGTG
GGACGAGTCC AGATGCGGAC GCGGCGGACG TTAAGGGGAC ACCTGGCCAA GATTTACGCC
ATGCACTGGG CCACTGATTC TAAGCTGCTG GTAAGTGCCT CGCAAGATGG GAAGCTGATC
GTGTGGGACA GCTACACCAC CAACAAGGTG CACGCCATCC CACTGCGCTC CTCCTGGGTC
ATGACCTGTG CCTATGCCCC ATCAGGGAAC TTTGTGGCAT GTGGGGGGCT GGACAACATG
TGTTCCATCT ACAACCTCAA ATCCCGTGAG GGCAATGTCA AGGTCAGCCG GGAGCTTTCT
GCTCACACAG GTTATCTCTC CTGCTGCCGC TTCCTGGATG ACAACAATAT TGTGACCAGC
TCGGGGGACA CCACGTGTGC CTTGTGGGAC ATTGAGACTG GGCAGCAGAA GACTGTATTT
GTGGGACACA CGGGTGACTG CATGAGCCTG GCTGTGTCTC CTGACTTCAA TCTCTTCATT
TCGGGGGCCT GTGATGCCAG TGCCAAGCTC TGGGATGTGC GAGAGGGGAC CTGCCGTCAG
ACTTTCACTG GCCACGAGTC GGACATCAAC GCCATCTGTT TCTTCCCCAA TGGAGAGGCC
ATCTGCACGG GCTCGGATGA CGCTTCCTGC CGCTTGTTTG ACCTGCGGGC AGACCAGGAG
CTGATCTGCT TCTCCCACGA GAGCATCATC TGCGGCATCA CGTCCGTGGC CTTCTCCCTC
AGTGGCCGCC TACTATTCGC TGGCTACGAC GACTTCAACT GCAATGTCTG GGACTCCATG
AAGTCTGAGC GTGTGGGCAT CCTCTCTGGC CACGATAACA GGGTGAGCTG CCTGGGAGTC
ACAGCTGACG GGATGGCTGT GGCCACAGGT TCCTGGGACA GCTTCCTCAA AATCTGGAAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_002066.1
CDS211..1233
Misc Feature(1)190..192(+)
Misc Feature(2)352..1230(+)
Misc Feature(3)355..1230(+)
Misc Feature(4)367..459(+)
Misc Feature(5)370..1230(+)
Misc Feature(6)493..585(+)
Misc Feature(7)595..597(+)
Misc Feature(8)631..720(+)
Misc Feature(9)754..846(+)
Misc Feature(10)880..972(+)
Misc Feature(11)1012..1104(+)
Misc Feature(12)1138..1230(+)
Exon (1)1..180
Gene:GNB3
Gene Synonym:
Exon (2)181..267
Gene:GNB3
Gene Synonym:
Exon (3)268..306
Gene:GNB3
Gene Synonym:
Exon (4)307..413
Gene:GNB3
Gene Synonym:
Exon (5)414..477
Gene:GNB3
Gene Synonym:
Exon (6)478..640
Gene:GNB3
Gene Synonym:
Exon (7)641..707
Gene:GNB3
Gene Synonym:
Exon (8)708..909
Gene:GNB3
Gene Synonym:
Exon (9)910..1126
Gene:GNB3
Gene Synonym:
Exon (10)1127..1730
Gene:GNB3
Gene Synonym:
Translation
Position Chain Variation Link
35 35 a, g dbSNP:2071057
86 86 a, t dbSNP:751757865
238 238 a, g dbSNP:754210939
243 243 a, g dbSNP:566098814
669 669 c, t dbSNP:200864177
830 830 a, c, g dbSNP:200634640
920 920 a, g dbSNP:113437937
936 936 c, g dbSNP:760856626
945 945 a, g dbSNP:112732503
951 951 c, t dbSNP:13306410
1024 1024 a, g dbSNP:5442
1035 1035 c, t dbSNP:5443
1080 1080 c, t dbSNP:753136154
1090 1090 a, t dbSNP:200733665
1351 1351 c, t dbSNP:5445
1633 1633 c, t dbSNP:5446
1661 1661 a, g dbSNP:774465135
1688 1688 a, t dbSNP:7975574

Target ORF information:

RefSeq Version NM_002075
Organism Homo sapiens (human)
Definition Homo sapiens guanine nucleotide binding protein (G protein), beta polypeptide 3 (GNB3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002075

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGGGGAGA TGGAGCAACT GCGTCAGGAA GCGGAGCAGC TCAAGAAGCA GATTGCAGAT 
GCCAGGAAAG CCTGTGCTGA CGTTACTCTG GCAGAGCTGG TGTCTGGCCT AGAGGTGGTG
GGACGAGTCC AGATGCGGAC GCGGCGGACG TTAAGGGGAC ACCTGGCCAA GATTTACGCC
ATGCACTGGG CCACTGATTC TAAGCTGCTG GTAAGTGCCT CGCAAGATGG GAAGCTGATC
GTGTGGGACA GCTACACCAC CAACAAGGTG CACGCCATCC CACTGCGCTC CTCCTGGGTC
ATGACCTGTG CCTATGCCCC ATCAGGGAAC TTTGTGGCAT GTGGGGGGCT GGACAACATG
TGTTCCATCT ACAACCTCAA ATCCCGTGAG GGCAATGTCA AGGTCAGCCG GGAGCTTTCT
GCTCACACAG GTTATCTCTC CTGCTGCCGC TTCCTGGATG ACAACAATAT TGTGACCAGC
TCGGGGGACA CCACGTGTGC CTTGTGGGAC ATTGAGACTG GGCAGCAGAA GACTGTATTT
GTGGGACACA CGGGTGACTG CATGAGCCTG GCTGTGTCTC CTGACTTCAA TCTCTTCATT
TCGGGGGCCT GTGATGCCAG TGCCAAGCTC TGGGATGTGC GAGAGGGGAC CTGCCGTCAG
ACTTTCACTG GCCACGAGTC GGACATCAAC GCCATCTGTT TCTTCCCCAA TGGAGAGGCC
ATCTGCACGG GCTCGGATGA CGCTTCCTGC CGCTTGTTTG ACCTGCGGGC AGACCAGGAG
CTGATCTGCT TCTCCCACGA GAGCATCATC TGCGGCATCA CGTCCGTGGC CTTCTCCCTC
AGTGGCCGCC TACTATTCGC TGGCTACGAC GACTTCAACT GCAATGTCTG GGACTCCATG
AAGTCTGAGC GTGTGGGCAT CCTCTCTGGC CACGATAACA GGGTGAGCTG CCTGGGAGTC
ACAGCTGACG GGATGGCTGT GGCCACAGGT TCCTGGGACA GCTTCCTCAA AATCTGGAAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28836
Accession Version NM_001297571.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU143349.1, AK313091.1, U47924.1 and BC015920.1. On Jul 3, 2014 this sequence version replaced gi:530398635. Summary: Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit which belongs to the WD repeat G protein beta family. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. A single-nucleotide polymorphism (C825T) in this gene is associated with essential hypertension and obesity. This polymorphism is also associated with the occurrence of the splice variant GNB3-s, which appears to have increased activity. GNB3-s is an example of alternative splicing caused by a nucleotide change outside of the splice donor and acceptor sites. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]. Transcript Variant: This variant (2) uses an alternate in-frame splice site, in the central coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK313091.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGGGAGA TGGAGCAACT GCGTCAGGAA GCGGAGCAGC TCAAGAAGCA GATTGCAGAT 
GCCAGGAAAG CCTGTGCTGA CGTTACTCTG GCAGAGCTGG TGTCTGGCCT AGAGGTGGTG
GGACGAGTCC AGATGCGGAC GCGGCGGACG TTAAGGGGAC ACCTGGCCAA GATTTACGCC
ATGCACTGGG CCACTGATTC TAAGCTGCTG GTAAGTGCCT CGCAAGATGG GAAGCTGATC
GTGTGGGACA GCTACACCAC CAACAAGGTG CACGCCATCC CACTGCGCTC CTCCTGGGTC
ATGACCTGTG CCTATGCCCC ATCAGGGAAC TTTGTGGCAT GTGGGGGGCT GGACAACATG
TGTTCCATCT ACAACCTCAA ATCCCGTGAG GGCAATGTCA AGGTCAGCCG GGAGCTTTCT
GCTCACACAG GTTATCTCTC CTGCTGCCGC TTCCTGGATG ACAACAATAT TGTGACCAGC
TCGGGGGACA CCACTGCCTT GTGGGACATT GAGACTGGGC AGCAGAAGAC TGTATTTGTG
GGACACACGG GTGACTGCAT GAGCCTGGCT GTGTCTCCTG ACTTCAATCT CTTCATTTCG
GGGGCCTGTG ATGCCAGTGC CAAGCTCTGG GATGTGCGAG AGGGGACCTG CCGTCAGACT
TTCACTGGCC ACGAGTCGGA CATCAACGCC ATCTGTTTCT TCCCCAATGG AGAGGCCATC
TGCACGGGCT CGGATGACGC TTCCTGCCGC TTGTTTGACC TGCGGGCAGA CCAGGAGCTG
ATCTGCTTCT CCCACGAGAG CATCATCTGC GGCATCACGT CCGTGGCCTT CTCCCTCAGT
GGCCGCCTAC TATTCGCTGG CTACGACGAC TTCAACTGCA ATGTCTGGGA CTCCATGAAG
TCTGAGCGTG TGGGCATCCT CTCTGGCCAC GATAACAGGG TGAGCTGCCT GGGAGTCACA
GCTGACGGGA TGGCTGTGGC CACAGGTTCC TGGGACAGCT TCCTCAAAAT CTGGAACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001284500.1
CDS211..1230
Misc Feature(1)190..192(+)
Misc Feature(2)352..1227(+)
Misc Feature(3)355..1227(+)
Misc Feature(4)367..459(+)
Misc Feature(5)370..1227(+)
Misc Feature(6)493..585(+)
Misc Feature(7)751..843(+)
Misc Feature(8)877..969(+)
Misc Feature(9)1009..1101(+)
Misc Feature(10)1135..1227(+)
Exon (1)1..180
Gene:GNB3
Gene Synonym:
Exon (2)181..267
Gene:GNB3
Gene Synonym:
Exon (3)268..306
Gene:GNB3
Gene Synonym:
Exon (4)307..413
Gene:GNB3
Gene Synonym:
Exon (5)414..477
Gene:GNB3
Gene Synonym:
Exon (6)478..640
Gene:GNB3
Gene Synonym:
Exon (7)641..704
Gene:GNB3
Gene Synonym:
Exon (8)705..906
Gene:GNB3
Gene Synonym:
Exon (9)907..1123
Gene:GNB3
Gene Synonym:
Exon (10)1124..1727
Gene:GNB3
Gene Synonym:
Translation
Position Chain Variation Link
35 35 a, g dbSNP:2071057
86 86 a, t dbSNP:751757865
238 238 a, g dbSNP:754210939
243 243 a, g dbSNP:566098814
669 669 c, t dbSNP:200864177
827 827 a, c, g dbSNP:200634640
917 917 a, g dbSNP:113437937
933 933 c, g dbSNP:760856626
942 942 a, g dbSNP:112732503
948 948 c, t dbSNP:13306410
1021 1021 a, g dbSNP:5442
1032 1032 c, t dbSNP:5443
1077 1077 c, t dbSNP:753136154
1087 1087 a, t dbSNP:200733665
1348 1348 c, t dbSNP:5445
1630 1630 c, t dbSNP:5446
1658 1658 a, g dbSNP:774465135
1685 1685 a, t dbSNP:7975574

Target ORF information:

RefSeq Version NM_001297571
Organism Homo sapiens (human)
Definition Homo sapiens guanine nucleotide binding protein (G protein), beta polypeptide 3 (GNB3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001297571

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGGGAGA TGGAGCAACT GCGTCAGGAA GCGGAGCAGC TCAAGAAGCA GATTGCAGAT 
GCCAGGAAAG CCTGTGCTGA CGTTACTCTG GCAGAGCTGG TGTCTGGCCT AGAGGTGGTG
GGACGAGTCC AGATGCGGAC GCGGCGGACG TTAAGGGGAC ACCTGGCCAA GATTTACGCC
ATGCACTGGG CCACTGATTC TAAGCTGCTG GTAAGTGCCT CGCAAGATGG GAAGCTGATC
GTGTGGGACA GCTACACCAC CAACAAGGTG CACGCCATCC CACTGCGCTC CTCCTGGGTC
ATGACCTGTG CCTATGCCCC ATCAGGGAAC TTTGTGGCAT GTGGGGGGCT GGACAACATG
TGTTCCATCT ACAACCTCAA ATCCCGTGAG GGCAATGTCA AGGTCAGCCG GGAGCTTTCT
GCTCACACAG GTTATCTCTC CTGCTGCCGC TTCCTGGATG ACAACAATAT TGTGACCAGC
TCGGGGGACA CCACTGCCTT GTGGGACATT GAGACTGGGC AGCAGAAGAC TGTATTTGTG
GGACACACGG GTGACTGCAT GAGCCTGGCT GTGTCTCCTG ACTTCAATCT CTTCATTTCG
GGGGCCTGTG ATGCCAGTGC CAAGCTCTGG GATGTGCGAG AGGGGACCTG CCGTCAGACT
TTCACTGGCC ACGAGTCGGA CATCAACGCC ATCTGTTTCT TCCCCAATGG AGAGGCCATC
TGCACGGGCT CGGATGACGC TTCCTGCCGC TTGTTTGACC TGCGGGCAGA CCAGGAGCTG
ATCTGCTTCT CCCACGAGAG CATCATCTGC GGCATCACGT CCGTGGCCTT CTCCCTCAGT
GGCCGCCTAC TATTCGCTGG CTACGACGAC TTCAACTGCA ATGTCTGGGA CTCCATGAAG
TCTGAGCGTG TGGGCATCCT CTCTGGCCAC GATAACAGGG TGAGCTGCCT GGGAGTCACA
GCTGACGGGA TGGCTGTGGC CACAGGTTCC TGGGACAGCT TCCTCAAAAT CTGGAACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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