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GNGT1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol GNGT1
Entrez Gene ID 2792
Full Name guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1
Synonyms GNG1
General protein information
Preferred Names
guanine nucleotide-binding protein G(T) subunit gamma-T1
Names
guanine nucleotide-binding protein G(T) subunit gamma-T1
transducin gamma chain
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7q21.3

Summary Heterotrimeric guanine nucleotide-binding proteins (G proteins) transduce extracellular signals received by transmembrane receptors to effector proteins. Transducin is a guanine nucleotide-binding protein found specifically in rod outer segments, where it mediates activation by rhodopsin of a cyclic GTP-specific (guanosine monophosphate) phosphodiesterase. Transducin is also referred to as GMPase. GNGT1 encodes the gamma subunit of transducin (Hurley et al., 1984 [PubMed 6438626]; Scherer et al., 1996 [PubMed 8661128]).[supplied by OMIM, Mar 2008]. lac of sum
Disorder MIM:

189970

Disorder Html:

mRNA and Protein(s)

mRNA Protein Name
NM_021955 NP_068774 guanine nucleotide-binding protein G(T) subunit gamma-T1 precursor

KEGG
hsa05200 Pathways in cancer
hsa04062 Chemokine signaling pathway
hsa04744 Phototransduction
hsa04725 Cholinergic synapse
hsa04724 Glutamatergic synapse
hsa04727 GABAergic synapse
hsa04728 Dopaminergic synapse
hsa04726 Serotonergic synapse
hsa04723 Retrograde endocannabinoid signaling
hsa05032 Morphine addiction
hsa04151 PI3K-Akt signaling pathway
hsa05034 Alcoholism
hsa04713 Circadian entrainment
hsa04014 Ras signaling pathway
Reactome
R-HSA-112316 Neuronal System
R-HSA-112315 Transmission across Chemical Synapses
R-HSA-1296071 Potassium Channels
R-HSA-112314 Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell
R-HSA-1296065 Inwardly rectifying K+ channels
R-HSA-1296041 Activation of G protein gated Potassium channels
R-HSA-977444 GABA B receptor activation
R-HSA-991365 Activation of GABAB receptors
R-HSA-500657 Presynaptic function of Kainate receptors
R-HSA-997272 Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits
R-HSA-977443 GABA receptor activation
R-HSA-451326 Activation of Kainate Receptors upon glutamate binding
R-HSA-1296059 G protein gated Potassium channels
R-HSA-109582 Hemostasis
R-HSA-418346 Platelet homeostasis
R-HSA-428930 Thromboxane signalling through TP receptor
R-HSA-392851 Prostacyclin signalling through prostacyclin receptor
R-HSA-418592 ADP signalling through P2Y purinoceptor 1
R-HSA-456926 Thrombin signalling through proteinase activated receptors (PARs)
R-HSA-162582 Signal Transduction
R-HSA-392518 Signal amplification
R-HSA-76002 Platelet activation, signaling and aggregation
R-HSA-392170 ADP signalling through P2Y purinoceptor 12
R-HSA-500792 GPCR ligand binding
R-HSA-420092 Glucagon-type ligand receptors
R-HSA-388396 GPCR downstream signaling
R-HSA-418594 G alpha (i) signalling events
R-HSA-372790 Signaling by GPCR
R-HSA-418597 G alpha (z) signalling events
R-HSA-392451 G beta:gamma signalling through PI3Kgamma
R-HSA-381753 Olfactory Signaling Pathway
R-HSA-373080 Class B/2 (Secretin family receptors)
R-HSA-111885 Opioid Signalling
R-HSA-416482 G alpha (12/13) signalling events
R-HSA-881907 Gastrin-CREB signalling pathway via PKC and MAPK
R-HSA-418555 G alpha (s) signalling events
R-HSA-418217 G beta:gamma signalling through PLC beta
R-HSA-416476 G alpha (q) signalling events
R-HSA-3858494 Beta-catenin independent WNT signaling
R-HSA-195721 Signaling by Wnt
R-HSA-397795 G-protein beta:gamma signalling
R-HSA-2187338 Visual phototransduction
R-HSA-4086398 Ca2+ pathway
R-HSA-2514856 The phototransduction cascade
R-HSA-2514859 Inactivation, recovery and regulation of the phototransduction cascade
R-HSA-202040 G-protein activation
R-HSA-2485179 Activation of the phototransduction cascade
R-HSA-1430728 Metabolism
R-HSA-382551 Transmembrane transport of small molecules
R-HSA-445717 Aquaporin-mediated transport
R-HSA-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-HSA-163685 Integration of energy metabolism
R-HSA-422356 Regulation of insulin secretion
R-HSA-381676 Glucagon-like Peptide-1 (GLP1) regulates insulin secretion
R-HSA-400042 Adrenaline,noradrenaline inhibits insulin secretion
Pathway Interaction Database
rhodopsin_pathway Visual signal transduction: Rods
WikiPathways
WP289 Myometrial Relaxation and Contraction Pathways
WP712 Estrogen signaling pathway
WP35 G Protein Signaling Pathways
WP536 Calcium Regulation in the Cardiac Cell


Homo sapiens (human) GNGT1 NP_068774.1
Pan troglodytes (chimpanzee) GNGT1 XP_001168330.1
Macaca mulatta (Rhesus monkey) GNGT1 XP_001098610.2
Canis lupus familiaris (dog) GNGT1 NP_001003225.1
Bos taurus (cattle) GNGT1 NP_776752.1
Mus musculus (house mouse) Gngt1 NP_034444.1
Rattus norvegicus (Norway rat) Gngt1 NP_001129249.1
Danio rerio (zebrafish) gngt1 NP_956261.1
Xenopus (Silurana) tropicalis (western clawed frog) gngt1 NP_001165112.1

Component

ID Name Evidence
GO:0005834 heterotrimeric G-protein complex IEA
GO:0005886 plasma membrane EXP
GO:0005886 plasma membrane TAS

Function

ID Name Evidence
GO:0003924 GTPase activity TAS
GO:0004871 signal transducer activity IEA

Process

ID Name Evidence
GO:0006184 GTP catabolic process TAS
GO:0007165 signal transduction NAS
GO:0007186 G-protein coupled receptor protein signaling pathway IEA
GO:0007268 synaptic transmission TAS
GO:0007602 phototransduction IEA
GO:0008104 protein localization IEA
GO:0042462 eye photoreceptor cell development IEA

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GNGT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNGT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu04853 NM_021955 Homo sapiens guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1 (GNGT1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $99.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee that the protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OHu04853
Accession Version NM_021955.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 225bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product guanine nucleotide-binding protein G(T) subunit gamma-T1 precursor
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC029367.1 and AC002076.2. On Sep 9, 2005 this sequence version replaced gi:41584205. Summary: Heterotrimeric guanine nucleotide-binding proteins (G proteins) transduce extracellular signals received by transmembrane receptors to effector proteins. Transducin is a guanine nucleotide-binding protein found specifically in rod outer segments, where it mediates activation by rhodopsin of a cyclic GTP-specific (guanosine monophosphate) phosphodiesterase. Transducin is also referred to as GMPase. GNGT1 encodes the gamma subunit of transducin (Hurley et al., 1984 [PubMed 6438626]; Scherer et al., 1996 [PubMed 8661128]).[supplied by OMIM, Mar 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG435040.1, EL946418.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149398, SAMEA2153307 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
ATGCCAGTAA TCAATATTGA GGACCTGACA GAAAAGGACA AATTGAAGAT GGAAGTTGAC 
CAGCTCAAGA AAGAAGTGAC ACTGGAAAGA ATGCTAGTTT CCAAATGTTG TGAAGAAGTA
AGAGATTACG TTGAAGAACG ATCTGGCGAG GATCCACTGG TAAAGGGCAT CCCAGAGGAC
AAAAATCCCT TCAAGGAGCT CAAAGGAGGC TGTGTGATTT CATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_068774.1
CDS149..373
Misc Feature(1)110..112(+)
Misc Feature(2)182..352(+)
Misc Feature(3)182..340(+)
Exon (1)1..137
Gene:GNGT1
Gene Synonym:
Exon (2)138..244
Gene:GNGT1
Gene Synonym:
Exon (3)245..628
Gene:GNGT1
Gene Synonym:
Translation
Position Chain Variation Link
39 39 c, t dbSNP:564346446
80 80 c, t dbSNP:147350641
91 91 c, t dbSNP:573732840
108 108 g, t dbSNP:542579365
140 140 c, g dbSNP:747966528
143 143 a, c dbSNP:540836125
148 148 a, g dbSNP:778843372
152 152 a, c dbSNP:746027091
153 153 c, t dbSNP:376703142
154 154 a, g dbSNP:772250512
156 156 g, t dbSNP:775641362
157 157 a, g dbSNP:191685430
163 163 c, t dbSNP:768944512
177 177 c, g, t dbSNP:776996322
185 185 a, c, g dbSNP:765842422
203 203 g, t dbSNP:762577190
205 205 c, t dbSNP:150956934
207 207 a, c dbSNP:751213457
209 209 c, g dbSNP:141065761
211 211 a, g dbSNP:781074304
224 224 g, t dbSNP:752594162
225 225 c, t dbSNP:755999687
230 230 a, c, t dbSNP:200035274
239 239 a, t dbSNP:772100965
240 240 c, t dbSNP:780114005
244 244 a, g dbSNP:202009903
259 259 c, t dbSNP:749751598
265 265 a, c, g dbSNP:202213169
278 278 a, c, g dbSNP:149827734
280 280 c, t dbSNP:767054727
282 282 a, g dbSNP:146833932
286 286 a, g dbSNP:374057761
287 287 c, t dbSNP:532358490
288 288 a, g dbSNP:535998188
290 290 c, t dbSNP:753632527
295 295 c, t dbSNP:552036514
296 296 a, c, g dbSNP:17243826
297 297 a, g dbSNP:754998799
301 301 c, t dbSNP:781165241
303 303 c, t dbSNP:748339515
304 304 a, g dbSNP:756306717
306 306 c, t dbSNP:778189581
307 307 a, g dbSNP:749739233
308 308 c, g dbSNP:771534540
309 309 c, t dbSNP:775047653
311 311 a, g dbSNP:746399500
313 313 a, g dbSNP:554239065
314 314 g, t dbSNP:771543991
315 315 g, t dbSNP:138254553
321 321 c, t dbSNP:760360120
323 323 a, g dbSNP:763718758
324 324 a, g dbSNP:776376731
340 340 a, c dbSNP:149136992
350 350 a, g dbSNP:765035469
358 358 c, t dbSNP:750316971
360 360 a, g dbSNP:143310057
361 361 c, t dbSNP:368033084
374 374 a, t dbSNP:767579385
386 386 -, a dbSNP:748949063
387 387 -, a dbSNP:768246904
393 393 a, t dbSNP:752779933
397 397 -, aaac dbSNP:774725344
398 398 a, c dbSNP:77925896
400 400 c, t dbSNP:777898529
406 406 a, c dbSNP:372563644
407 407 g, t dbSNP:757647866
408 408 a, t dbSNP:779423987
423 423 c, t dbSNP:746317789
429 429 c, t dbSNP:554472946
432 432 c, g dbSNP:748499860
439 439 a, g dbSNP:772631015
449 449 a, c dbSNP:778205326
455 455 a, g dbSNP:115265988
460 460 c, t dbSNP:747056537
464 464 a, g dbSNP:141229133
476 476 c, t dbSNP:774254934
477 477 a, c, g dbSNP:79786664
491 491 a, c, g, t dbSNP:2798
497 497 a, t dbSNP:542372552
504 504 c, t dbSNP:562148252
515 515 a, c dbSNP:572487900
531 531 c, t dbSNP:370988411
544 544 c, g dbSNP:537434585
586 586 c, t dbSNP:560398039

Target ORF information:

RefSeq Version NM_021955
Organism Homo sapiens (human)
Definition Homo sapiens guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1 (GNGT1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_021955

ORF Insert Sequence:

1
61
121
181
ATGCCAGTAA TCAATATTGA GGACCTGACA GAAAAGGACA AATTGAAGAT GGAAGTTGAC 
CAGCTCAAGA AAGAAGTGAC ACTGGAAAGA ATGCTAGTTT CCAAATGTTG TGAAGAAGTA
AGAGATTACG TTGAAGAACG ATCTGGCGAG GATCCACTGG TAAAGGGCAT CCCAGAGGAC
AAAAATCCCT TCAAGGAGCT CAAAGGAGGC TGTGTGATTT CATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

IGF-1 activates a cilium-localized noncanonical Gbetagamma signaling pathway that regulates cell-cycle progression
Dev. Cell 26 (4), 358-368 (2013)
Yeh C, Li A, Chuang JZ, Saito M, Caceres A and Sung CH.


book

Genome-wide association study of lung function decline in adults with and without asthma
J. Allergy Clin. Immunol. 129 (5), 1218-1228 (2012)
Imboden M, Bouzigon E, Curjuric I, Ramasamy A, Kumar A, Hancock DB, Wilk JB, Vonk JM, Thun GA, Siroux V, Nadif R, Monier F, Gonzalez JR, Wjst M, Heinrich J, Loehr LR, Franceschini N, North KE, Altmuller J, Koppelman GH, Guerra S, Kronenberg F, Lathrop M, Moffatt MF, O'Connor GT, Strachan DP, Postma DS, London SJ, Schindler C, Kogevinas M, Kauffmann F, Jarvis DL, Demenais F and Probst-Hensch NM.


book

The WD40 repeat protein WDR26 binds Gbetagamma and promotes Gbetagamma-dependent signal transduction and leukocyte migration
J. Biol. Chem. 286 (51), 43902-43912 (2011)
Sun Z, Tang X, Lin F and Chen S.


book

G protein betagamma subunits regulate cell adhesion through Rap1a and its effector Radil
J. Biol. Chem. 285 (9), 6538-6551 (2010)
Ahmed SM, Daulat AM, Meunier A and Angers S.


book

G Protein betagamma subunits augment UVB-induced apoptosis by stimulating the release of soluble heparin-binding epidermal growth factor from human keratinocytes
J. Biol. Chem. 282 (34), 24720-24730 (2007)
Seo M, Lee MJ, Heo JH, Lee YI, Kim Y, Kim SY, Lee ES and Juhnn YS.


book

Differential ability to form the G protein betagamma complex among members of the beta and gamma subunit families
J. Biol. Chem. 271 (12), 7141-7146 (1996)
Yan K, Kalyanaraman V and Gautam N.


book

Isolation of cDNA clones encoding eight different human G protein gamma subunits, including three novel forms designated the gamma 4, gamma 10, and gamma 11 subunits
J. Biol. Chem. 270 (37), 21765-21771 (1995)
Ray K, Kunsch C, Bonner LM and Robishaw JD.


book

Structure of the bovine transducin gamma subunit gene and analysis of promoter function in transgenic mice
Exp. Eye Res. 56 (4), 497-507 (1993)
Tao L, Pandey S, Simon MI and Fong HK.


book

G-protein beta gamma dimers. Membrane targeting requires subunit coexpression and intact gamma C-A-A-X domain
J. Biol. Chem. 266 (9), 5363-5366 (1991)
Simonds WF, Butrynski JE, Gautam N, Unson CG and Spiegel AM.


book

Isolation and characterization of a cDNA clone for the gamma subunit of bovine retinal transducin
Proc. Natl. Acad. Sci. U.S.A. 81 (22), 6948-6952 (1984)
Hurley,J.B., Fong,H.K., Teplow,D.B., Dreyer,W.J. and Simon,M.I.


 
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