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GRIN2B cDNA ORF clone, Homo sapiens (human)

Gene Symbol GRIN2B
Entrez Gene ID 2904
Full Name glutamate receptor, ionotropic, N-methyl D-aspartate 2B
Synonyms EIEE27, GluN2B, MRD6, NMDAR2B, NR2B, hNR3
General protein information
Preferred Names
glutamate receptor ionotropic, NMDA 2B
Names
glutamate receptor ionotropic, NMDA 2B
NR3
glutamate receptor subunit epsilon-2
N-methyl-D-aspartate receptor subunit 3
N-methyl D-aspartate receptor subtype 2B
glutamate [NMDA] receptor subunit epsilon-2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12p12

Summary N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

138252


mRNA and Protein(s)

mRNA Protein Name
XM_011520628 XP_011518930 glutamate receptor ionotropic, NMDA 2B isoform X1
XM_011520629 XP_011518931 glutamate receptor ionotropic, NMDA 2B isoform X1
XM_011520630 XP_011518932 glutamate receptor ionotropic, NMDA 2B isoform X1
XM_005253351 XP_005253408 glutamate receptor ionotropic, NMDA 2B isoform X2
NM_000834 NP_000825 glutamate receptor ionotropic, NMDA 2B precursor



Homo sapiens (human) GRIN2B NP_000825.2
Pan troglodytes (chimpanzee) GRIN2B XP_528744.2
Macaca mulatta (Rhesus monkey) GRIN2B XP_001088140.1
Canis lupus familiaris (dog) GRIN2B NP_001008719.1
Bos taurus (cattle) GRIN2B NP_001179850.1
Mus musculus (house mouse) Grin2b NP_032197.3
Rattus norvegicus (Norway rat) Grin2b NP_036706.1
Gallus gallus (chicken) GRIN2B XP_416204.2
Danio rerio (zebrafish) LOC559976 NP_001121809.1
Danio rerio (zebrafish) LOC100334883 XP_003198060.2
Xenopus (Silurana) tropicalis (western clawed frog) grin2b XP_002932208.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GRIN2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRIN2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu26128
XM_011520628 PREDICTED: Homo sapiens glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$519.50-$727.30
$1039.00
OHu26128
XM_011520629 PREDICTED: Homo sapiens glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$519.50-$727.30
$1039.00
OHu26128
XM_011520630 PREDICTED: Homo sapiens glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$519.50-$727.30
$1039.00
OHu46089 XM_005253351 PREDICTED: Homo sapiens glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00
OHu26128
NM_000834 Homo sapiens glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$519.50-$727.30
$1039.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu26128
Clone ID Related Accession (Same CDS sequence) NM_000834 , XM_011520628 , XM_011520629 , XM_011520630
Accession Version XM_011520628.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4455bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product glutamate receptor ionotropic, NMDA 2B isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009714.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
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481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGAAGCCCA GAGCGGAGTG CTGTTCTCCC AAGTTCTGGT TGGTGTTGGC CGTCCTGGCC 
GTGTCAGGCA GCAGAGCTCG TTCTCAGAAG AGCCCCCCCA GCATTGGCAT TGCTGTCATC
CTCGTGGGCA CTTCCGACGA GGTGGCCATC AAGGATGCCC ACGAGAAAGA TGATTTCCAC
CATCTCTCCG TGGTACCCCG GGTGGAACTG GTAGCCATGA ATGAGACCGA CCCAAAGAGC
ATCATCACCC GCATCTGTGA TCTCATGTCT GACCGGAAGA TCCAGGGGGT GGTGTTTGCT
GATGACACAG ACCAGGAAGC CATCGCCCAG ATCCTCGATT TCATTTCAGC ACAGACTCTC
ACCCCCATCC TGGGCATCCA CGGGGGCTCC TCTATGATAA TGGCAGATAA GGATGAATCC
TCCATGTTCT TCCAGTTTGG CCCATCAATT GAACAGCAAG CTTCCGTAAT GCTCAACATC
ATGGAAGAAT ATGACTGGTA CATCTTTTCT ATCGTCACCA CCTATTTCCC TGGCTACCAG
GACTTTGTAA ACAAGATCCG CAGCACCATT GAGAATAGCT TTGTGGGCTG GGAGCTAGAG
GAGGTCCTCC TACTGGACAT GTCCCTGGAC GATGGAGATT CTAAGATCCA GAATCAGCTC
AAGAAACTTC AAAGCCCCAT CATTCTTCTT TACTGTACCA AGGAAGAAGC CACCTACATC
TTTGAAGTGG CCAACTCAGT AGGGCTGACT GGCTATGGCT ACACGTGGAT CGTGCCCAGT
CTGGTGGCAG GGGATACAGA CACAGTGCCT GCGGAGTTCC CCACTGGGCT CATCTCTGTA
TCATATGATG AATGGGACTA TGGCCTCCCC GCCAGAGTGA GAGATGGAAT TGCCATAATC
ACCACTGCTG CTTCTGACAT GCTGTCTGAG CACAGCTTCA TCCCTGAGCC CAAAAGCAGT
TGTTACAACA CCCACGAGAA GAGAATCTAC CAGTCCAATA TGCTAAATAG GTATCTGATC
AATGTCACTT TTGAGGGGAG GAATTTGTCC TTCAGTGAAG ATGGCTACCA GATGCACCCG
AAACTGGTGA TAATTCTTCT GAACAAGGAG AGGAAGTGGG AAAGGGTGGG GAAGTGGAAA
GACAAGTCCC TGCAGATGAA GTACTATGTG TGGCCCCGAA TGTGTCCAGA GACTGAAGAG
CAGGAGGATG ACCATCTGAG CATTGTGACC CTGGAGGAGG CACCATTTGT CATTGTGGAA
AGTGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACAG TCCCCTGCCA AAAACGCATA
GTCACTGAGA ATAAAACAGA CGAGGAGCCG GGTTACATCA AAAAATGCTG CAAGGGGTTC
TGTATTGACA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA CCTTTACCTG
GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG
GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGCTCACTCA CCATCAATGA GGAACGATCG
GAGGTGGTCG ACTTCTCTGT GCCCTTCATA GAGACAGGCA TCAGTGTCAT GGTGTCACGC
AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTATGGGTG
ATGATGTTTG TGATGCTGCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT TGAGTACTTC
AGCCCTGTGG GTTATAACAG GTGCCTCGCT GATGGCAGAG AGCCTGGTGG ACCCTCTTTC
ACCATCGGCA AAGCTATTTG GTTGCTCTGG GGTCTGGTGT TTAACAACTC CGTACCTGTG
CAGAACCCAA AGGGGACCAC CTCCAAGATC ATGGTGTCAG TGTGGGCCTT CTTTGCTGTC
ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAATATGTG
GACCAGGTTT CTGGCCTGAG CGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
TTCCGCTTTG GGACCGTGCC CAACGGCAGC ACAGAGAGAA ATATTCGCAA TAACTATGCA
GAAATGCATG CCTACATGGG AAAGTTCAAC CAGAGGGGTG TAGATGATGC ATTGCTCTCC
CTGAAAACAG GGAAACTGGA TGCCTTCATC TATGATGCAG CAGTGCTGAA CTATATGGCA
GGCAGAGATG AAGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCTTCCACT
GGCTATGGCA TTGCCATCCA AAAAGATTCT GGGTGGAAGC GCCAGGTGGA CCTTGCTATC
CTGCAGCTCT TTGGAGATGG GGAGATGGAA GAACTGGAAG CTCTCTGGCT CACTGGCATT
TGTCACAATG AGAAGAATGA GGTCATGAGC AGCCAGCTGG ACATTGACAA CATGGCAGGG
GTCTTCTACA TGTTGGGGGC GGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGCGAACAC
CTTTTCTATT GGCAGTTCCG ACATTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG
GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GGGTGGCGAT CGAGGAGCGC
CAGTCTGTAA TGAACTCCCC CACCGCAACC ATGAACAACA CACACTCCAA CATCCTGCGC
CTGCTGCGCA CGGCCAAGAA CATGGCTAAC CTGTCTGGTG TGAATGGCTC ACCGCAGAGC
GCCCTGGACT TCATCCGACG GGAGTCATCC GTCTATGACA TCTCAGAGCA CCGCCGCAGC
TTCACGCATT CTGACTGCAA ATCCTACAAC AACCCGCCCT GTGAGGAGAA CCTCTTCAGT
GACTACATCA GTGAGGTAGA GAGAACGTTC GGGAACCTGC AGCTGAAGGA CAGCAACGTG
TACCAAGATC ACTACCACCA TCACCACCGG CCCCATAGTA TTGGCAGTGC CAGCTCCATC
GATGGGCTCT ACGACTGTGA CAACCCACCC TTCACCACCC AGTCCAGGTC CATCAGCAAG
AAGCCCCTGG ACATCGGCCT CCCCTCCTCC AAGCACAGCC AGCTCAGTGA CCTGTACGGC
AAATTCTCCT TCAAGAGCGA CCGCTACAGT GGCCACGACG ACTTGATCCG CTCCGATGTC
TCTGACATCT CAACCCACAC CGTCACCTAT GGGAACATCG AGGGCAATGC CGCCAAGAGG
CGTAAGCAGC AATATAAGGA CAGCCTGAAG AAGCGGCCTG CCTCGGCCAA GTCCCGCAGG
GAGTTTGACG AGATCGAGCT GGCCTACCGT CGCCGACCGC CCCGCTCCCC TGACCACAAG
CGCTACTTCA GGGACAAGGA AGGGCTACGG GACTTCTACC TGGACCAGTT CCGAACAAAG
GAGAACTCAC CCCACTGGGA GCACGTAGAC CTGACCGACA TCTACAAGGA GCGGAGTGAT
GACTTTAAGC GCGACTCCGT CAGCGGAGGA GGGCCCTGTA CCAACAGGTC TCACATCAAG
CACGGGACGG GCGACAAACA CGGCGTGGTC AGCGGGGTAC CTGCACCTTG GGAGAAGAAC
CTGACCAACG TGGAGTGGGA GGACCGGTCC GGGGGCAACT TCTGCCGCAG CTGTCCCTCC
AAGCTGCACA ACTACTCCAC GACGGTGACG GGTCAGAACT CGGGCAGGCA GGCGTGCATC
CGGTGTGAGG CTTGCAAGAA AGCAGGCAAC CTGTATGACA TCAGTGAGGA CAACTCCCTG
CAGGAACTGG ACCAGCCGGC TGCCCCAGTG GCGGTGACGT CAAACGCCTC CACCACTAAG
TACCCTCAGA GCCCGACTAA TTCCAAGGCC CAGAAGAAGA ACCGGAACAA ACTGCGCCGG
CAGCACTCCT ACGACACCTT CGTGGACCTG CAGAAGGAAG AAGCCGCCCT GGCCCCGCGC
AGCGTAAGCC TGAAAGACAA GGGCCGATTC ATGGATGGGA GCCCCTACGC CCACATGTTT
GAGATGTCAG CTGGCGAGAG CACCTTTGCC AACAACAAGT CCTCAGTGCC CACTGCCGGA
CATCACCACC ACAACAACCC CGGCGGCGGG TACATGCTCA GCAAGTCGCT CTACCCTGAC
CGGGTCACGC AAAACCCTTT CATCCCCACT TTTGGGGACG ACCAGTGCTT GCTCCATGGC
AGCAAATCCT ACTTCTTCAG GCAGCCCACG GTGGCGGGGG CGTCGAAAGC CAGGCCGGAC
TTCCGGGCCC TTGTCACCAA CAAGCCGGTG GTCTCGGCCC TTCATGGGGC CGTGCCAGCC
CGTTTCCAGA AGGACATCTG TATAGGGAAC CAGTCCAACC CCTGTGTGCC TAACAACAAA
AACCCCAGGG CTTTCAATGG CTCCAGCAAT GGGCATGTTT ATGAGAAACT TTCTAGTATT
GAGTCTGATG TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011518930.1
CDS747..5201
Misc Feature(1)843..1925(+)
Misc Feature(2)981..1367(+)
Misc Feature(3)1953..3155(+)
Misc Feature(4)2121..>2372(+)
Misc Feature(5)2280..3116(+)
Misc Feature(6)2280..2942(+)
Misc Feature(7)2415..3233(+)
Misc Feature(8)3264..5198(+)
Translation

Target ORF information:

RefSeq Version XM_011520628
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011520628

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGAAGCCCA GAGCGGAGTG CTGTTCTCCC AAGTTCTGGT TGGTGTTGGC CGTCCTGGCC 
GTGTCAGGCA GCAGAGCTCG TTCTCAGAAG AGCCCCCCCA GCATTGGCAT TGCTGTCATC
CTCGTGGGCA CTTCCGACGA GGTGGCCATC AAGGATGCCC ACGAGAAAGA TGATTTCCAC
CATCTCTCCG TGGTACCCCG GGTGGAACTG GTAGCCATGA ATGAGACCGA CCCAAAGAGC
ATCATCACCC GCATCTGTGA TCTCATGTCT GACCGGAAGA TCCAGGGGGT GGTGTTTGCT
GATGACACAG ACCAGGAAGC CATCGCCCAG ATCCTCGATT TCATTTCAGC ACAGACTCTC
ACCCCCATCC TGGGCATCCA CGGGGGCTCC TCTATGATAA TGGCAGATAA GGATGAATCC
TCCATGTTCT TCCAGTTTGG CCCATCAATT GAACAGCAAG CTTCCGTAAT GCTCAACATC
ATGGAAGAAT ATGACTGGTA CATCTTTTCT ATCGTCACCA CCTATTTCCC TGGCTACCAG
GACTTTGTAA ACAAGATCCG CAGCACCATT GAGAATAGCT TTGTGGGCTG GGAGCTAGAG
GAGGTCCTCC TACTGGACAT GTCCCTGGAC GATGGAGATT CTAAGATCCA GAATCAGCTC
AAGAAACTTC AAAGCCCCAT CATTCTTCTT TACTGTACCA AGGAAGAAGC CACCTACATC
TTTGAAGTGG CCAACTCAGT AGGGCTGACT GGCTATGGCT ACACGTGGAT CGTGCCCAGT
CTGGTGGCAG GGGATACAGA CACAGTGCCT GCGGAGTTCC CCACTGGGCT CATCTCTGTA
TCATATGATG AATGGGACTA TGGCCTCCCC GCCAGAGTGA GAGATGGAAT TGCCATAATC
ACCACTGCTG CTTCTGACAT GCTGTCTGAG CACAGCTTCA TCCCTGAGCC CAAAAGCAGT
TGTTACAACA CCCACGAGAA GAGAATCTAC CAGTCCAATA TGCTAAATAG GTATCTGATC
AATGTCACTT TTGAGGGGAG GAATTTGTCC TTCAGTGAAG ATGGCTACCA GATGCACCCG
AAACTGGTGA TAATTCTTCT GAACAAGGAG AGGAAGTGGG AAAGGGTGGG GAAGTGGAAA
GACAAGTCCC TGCAGATGAA GTACTATGTG TGGCCCCGAA TGTGTCCAGA GACTGAAGAG
CAGGAGGATG ACCATCTGAG CATTGTGACC CTGGAGGAGG CACCATTTGT CATTGTGGAA
AGTGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACAG TCCCCTGCCA AAAACGCATA
GTCACTGAGA ATAAAACAGA CGAGGAGCCG GGTTACATCA AAAAATGCTG CAAGGGGTTC
TGTATTGACA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA CCTTTACCTG
GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG
GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGCTCACTCA CCATCAATGA GGAACGATCG
GAGGTGGTCG ACTTCTCTGT GCCCTTCATA GAGACAGGCA TCAGTGTCAT GGTGTCACGC
AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTATGGGTG
ATGATGTTTG TGATGCTGCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT TGAGTACTTC
AGCCCTGTGG GTTATAACAG GTGCCTCGCT GATGGCAGAG AGCCTGGTGG ACCCTCTTTC
ACCATCGGCA AAGCTATTTG GTTGCTCTGG GGTCTGGTGT TTAACAACTC CGTACCTGTG
CAGAACCCAA AGGGGACCAC CTCCAAGATC ATGGTGTCAG TGTGGGCCTT CTTTGCTGTC
ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAATATGTG
GACCAGGTTT CTGGCCTGAG CGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
TTCCGCTTTG GGACCGTGCC CAACGGCAGC ACAGAGAGAA ATATTCGCAA TAACTATGCA
GAAATGCATG CCTACATGGG AAAGTTCAAC CAGAGGGGTG TAGATGATGC ATTGCTCTCC
CTGAAAACAG GGAAACTGGA TGCCTTCATC TATGATGCAG CAGTGCTGAA CTATATGGCA
GGCAGAGATG AAGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCTTCCACT
GGCTATGGCA TTGCCATCCA AAAAGATTCT GGGTGGAAGC GCCAGGTGGA CCTTGCTATC
CTGCAGCTCT TTGGAGATGG GGAGATGGAA GAACTGGAAG CTCTCTGGCT CACTGGCATT
TGTCACAATG AGAAGAATGA GGTCATGAGC AGCCAGCTGG ACATTGACAA CATGGCAGGG
GTCTTCTACA TGTTGGGGGC GGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGCGAACAC
CTTTTCTATT GGCAGTTCCG ACATTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG
GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GGGTGGCGAT CGAGGAGCGC
CAGTCTGTAA TGAACTCCCC CACCGCAACC ATGAACAACA CACACTCCAA CATCCTGCGC
CTGCTGCGCA CGGCCAAGAA CATGGCTAAC CTGTCTGGTG TGAATGGCTC ACCGCAGAGC
GCCCTGGACT TCATCCGACG GGAGTCATCC GTCTATGACA TCTCAGAGCA CCGCCGCAGC
TTCACGCATT CTGACTGCAA ATCCTACAAC AACCCGCCCT GTGAGGAGAA CCTCTTCAGT
GACTACATCA GTGAGGTAGA GAGAACGTTC GGGAACCTGC AGCTGAAGGA CAGCAACGTG
TACCAAGATC ACTACCACCA TCACCACCGG CCCCATAGTA TTGGCAGTGC CAGCTCCATC
GATGGGCTCT ACGACTGTGA CAACCCACCC TTCACCACCC AGTCCAGGTC CATCAGCAAG
AAGCCCCTGG ACATCGGCCT CCCCTCCTCC AAGCACAGCC AGCTCAGTGA CCTGTACGGC
AAATTCTCCT TCAAGAGCGA CCGCTACAGT GGCCACGACG ACTTGATCCG CTCCGATGTC
TCTGACATCT CAACCCACAC CGTCACCTAT GGGAACATCG AGGGCAATGC CGCCAAGAGG
CGTAAGCAGC AATATAAGGA CAGCCTGAAG AAGCGGCCTG CCTCGGCCAA GTCCCGCAGG
GAGTTTGACG AGATCGAGCT GGCCTACCGT CGCCGACCGC CCCGCTCCCC TGACCACAAG
CGCTACTTCA GGGACAAGGA AGGGCTACGG GACTTCTACC TGGACCAGTT CCGAACAAAG
GAGAACTCAC CCCACTGGGA GCACGTAGAC CTGACCGACA TCTACAAGGA GCGGAGTGAT
GACTTTAAGC GCGACTCCGT CAGCGGAGGA GGGCCCTGTA CCAACAGGTC TCACATCAAG
CACGGGACGG GCGACAAACA CGGCGTGGTC AGCGGGGTAC CTGCACCTTG GGAGAAGAAC
CTGACCAACG TGGAGTGGGA GGACCGGTCC GGGGGCAACT TCTGCCGCAG CTGTCCCTCC
AAGCTGCACA ACTACTCCAC GACGGTGACG GGTCAGAACT CGGGCAGGCA GGCGTGCATC
CGGTGTGAGG CTTGCAAGAA AGCAGGCAAC CTGTATGACA TCAGTGAGGA CAACTCCCTG
CAGGAACTGG ACCAGCCGGC TGCCCCAGTG GCGGTGACGT CAAACGCCTC CACCACTAAG
TACCCTCAGA GCCCGACTAA TTCCAAGGCC CAGAAGAAGA ACCGGAACAA ACTGCGCCGG
CAGCACTCCT ACGACACCTT CGTGGACCTG CAGAAGGAAG AAGCCGCCCT GGCCCCGCGC
AGCGTAAGCC TGAAAGACAA GGGCCGATTC ATGGATGGGA GCCCCTACGC CCACATGTTT
GAGATGTCAG CTGGCGAGAG CACCTTTGCC AACAACAAGT CCTCAGTGCC CACTGCCGGA
CATCACCACC ACAACAACCC CGGCGGCGGG TACATGCTCA GCAAGTCGCT CTACCCTGAC
CGGGTCACGC AAAACCCTTT CATCCCCACT TTTGGGGACG ACCAGTGCTT GCTCCATGGC
AGCAAATCCT ACTTCTTCAG GCAGCCCACG GTGGCGGGGG CGTCGAAAGC CAGGCCGGAC
TTCCGGGCCC TTGTCACCAA CAAGCCGGTG GTCTCGGCCC TTCATGGGGC CGTGCCAGCC
CGTTTCCAGA AGGACATCTG TATAGGGAAC CAGTCCAACC CCTGTGTGCC TAACAACAAA
AACCCCAGGG CTTTCAATGG CTCCAGCAAT GGGCATGTTT ATGAGAAACT TTCTAGTATT
GAGTCTGATG TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26128
Clone ID Related Accession (Same CDS sequence) NM_000834 , XM_011520628 , XM_011520629 , XM_011520630
Accession Version XM_011520629.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4455bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product glutamate receptor ionotropic, NMDA 2B isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009714.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGAAGCCCA GAGCGGAGTG CTGTTCTCCC AAGTTCTGGT TGGTGTTGGC CGTCCTGGCC 
GTGTCAGGCA GCAGAGCTCG TTCTCAGAAG AGCCCCCCCA GCATTGGCAT TGCTGTCATC
CTCGTGGGCA CTTCCGACGA GGTGGCCATC AAGGATGCCC ACGAGAAAGA TGATTTCCAC
CATCTCTCCG TGGTACCCCG GGTGGAACTG GTAGCCATGA ATGAGACCGA CCCAAAGAGC
ATCATCACCC GCATCTGTGA TCTCATGTCT GACCGGAAGA TCCAGGGGGT GGTGTTTGCT
GATGACACAG ACCAGGAAGC CATCGCCCAG ATCCTCGATT TCATTTCAGC ACAGACTCTC
ACCCCCATCC TGGGCATCCA CGGGGGCTCC TCTATGATAA TGGCAGATAA GGATGAATCC
TCCATGTTCT TCCAGTTTGG CCCATCAATT GAACAGCAAG CTTCCGTAAT GCTCAACATC
ATGGAAGAAT ATGACTGGTA CATCTTTTCT ATCGTCACCA CCTATTTCCC TGGCTACCAG
GACTTTGTAA ACAAGATCCG CAGCACCATT GAGAATAGCT TTGTGGGCTG GGAGCTAGAG
GAGGTCCTCC TACTGGACAT GTCCCTGGAC GATGGAGATT CTAAGATCCA GAATCAGCTC
AAGAAACTTC AAAGCCCCAT CATTCTTCTT TACTGTACCA AGGAAGAAGC CACCTACATC
TTTGAAGTGG CCAACTCAGT AGGGCTGACT GGCTATGGCT ACACGTGGAT CGTGCCCAGT
CTGGTGGCAG GGGATACAGA CACAGTGCCT GCGGAGTTCC CCACTGGGCT CATCTCTGTA
TCATATGATG AATGGGACTA TGGCCTCCCC GCCAGAGTGA GAGATGGAAT TGCCATAATC
ACCACTGCTG CTTCTGACAT GCTGTCTGAG CACAGCTTCA TCCCTGAGCC CAAAAGCAGT
TGTTACAACA CCCACGAGAA GAGAATCTAC CAGTCCAATA TGCTAAATAG GTATCTGATC
AATGTCACTT TTGAGGGGAG GAATTTGTCC TTCAGTGAAG ATGGCTACCA GATGCACCCG
AAACTGGTGA TAATTCTTCT GAACAAGGAG AGGAAGTGGG AAAGGGTGGG GAAGTGGAAA
GACAAGTCCC TGCAGATGAA GTACTATGTG TGGCCCCGAA TGTGTCCAGA GACTGAAGAG
CAGGAGGATG ACCATCTGAG CATTGTGACC CTGGAGGAGG CACCATTTGT CATTGTGGAA
AGTGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACAG TCCCCTGCCA AAAACGCATA
GTCACTGAGA ATAAAACAGA CGAGGAGCCG GGTTACATCA AAAAATGCTG CAAGGGGTTC
TGTATTGACA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA CCTTTACCTG
GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG
GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGCTCACTCA CCATCAATGA GGAACGATCG
GAGGTGGTCG ACTTCTCTGT GCCCTTCATA GAGACAGGCA TCAGTGTCAT GGTGTCACGC
AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTATGGGTG
ATGATGTTTG TGATGCTGCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT TGAGTACTTC
AGCCCTGTGG GTTATAACAG GTGCCTCGCT GATGGCAGAG AGCCTGGTGG ACCCTCTTTC
ACCATCGGCA AAGCTATTTG GTTGCTCTGG GGTCTGGTGT TTAACAACTC CGTACCTGTG
CAGAACCCAA AGGGGACCAC CTCCAAGATC ATGGTGTCAG TGTGGGCCTT CTTTGCTGTC
ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAATATGTG
GACCAGGTTT CTGGCCTGAG CGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
TTCCGCTTTG GGACCGTGCC CAACGGCAGC ACAGAGAGAA ATATTCGCAA TAACTATGCA
GAAATGCATG CCTACATGGG AAAGTTCAAC CAGAGGGGTG TAGATGATGC ATTGCTCTCC
CTGAAAACAG GGAAACTGGA TGCCTTCATC TATGATGCAG CAGTGCTGAA CTATATGGCA
GGCAGAGATG AAGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCTTCCACT
GGCTATGGCA TTGCCATCCA AAAAGATTCT GGGTGGAAGC GCCAGGTGGA CCTTGCTATC
CTGCAGCTCT TTGGAGATGG GGAGATGGAA GAACTGGAAG CTCTCTGGCT CACTGGCATT
TGTCACAATG AGAAGAATGA GGTCATGAGC AGCCAGCTGG ACATTGACAA CATGGCAGGG
GTCTTCTACA TGTTGGGGGC GGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGCGAACAC
CTTTTCTATT GGCAGTTCCG ACATTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG
GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GGGTGGCGAT CGAGGAGCGC
CAGTCTGTAA TGAACTCCCC CACCGCAACC ATGAACAACA CACACTCCAA CATCCTGCGC
CTGCTGCGCA CGGCCAAGAA CATGGCTAAC CTGTCTGGTG TGAATGGCTC ACCGCAGAGC
GCCCTGGACT TCATCCGACG GGAGTCATCC GTCTATGACA TCTCAGAGCA CCGCCGCAGC
TTCACGCATT CTGACTGCAA ATCCTACAAC AACCCGCCCT GTGAGGAGAA CCTCTTCAGT
GACTACATCA GTGAGGTAGA GAGAACGTTC GGGAACCTGC AGCTGAAGGA CAGCAACGTG
TACCAAGATC ACTACCACCA TCACCACCGG CCCCATAGTA TTGGCAGTGC CAGCTCCATC
GATGGGCTCT ACGACTGTGA CAACCCACCC TTCACCACCC AGTCCAGGTC CATCAGCAAG
AAGCCCCTGG ACATCGGCCT CCCCTCCTCC AAGCACAGCC AGCTCAGTGA CCTGTACGGC
AAATTCTCCT TCAAGAGCGA CCGCTACAGT GGCCACGACG ACTTGATCCG CTCCGATGTC
TCTGACATCT CAACCCACAC CGTCACCTAT GGGAACATCG AGGGCAATGC CGCCAAGAGG
CGTAAGCAGC AATATAAGGA CAGCCTGAAG AAGCGGCCTG CCTCGGCCAA GTCCCGCAGG
GAGTTTGACG AGATCGAGCT GGCCTACCGT CGCCGACCGC CCCGCTCCCC TGACCACAAG
CGCTACTTCA GGGACAAGGA AGGGCTACGG GACTTCTACC TGGACCAGTT CCGAACAAAG
GAGAACTCAC CCCACTGGGA GCACGTAGAC CTGACCGACA TCTACAAGGA GCGGAGTGAT
GACTTTAAGC GCGACTCCGT CAGCGGAGGA GGGCCCTGTA CCAACAGGTC TCACATCAAG
CACGGGACGG GCGACAAACA CGGCGTGGTC AGCGGGGTAC CTGCACCTTG GGAGAAGAAC
CTGACCAACG TGGAGTGGGA GGACCGGTCC GGGGGCAACT TCTGCCGCAG CTGTCCCTCC
AAGCTGCACA ACTACTCCAC GACGGTGACG GGTCAGAACT CGGGCAGGCA GGCGTGCATC
CGGTGTGAGG CTTGCAAGAA AGCAGGCAAC CTGTATGACA TCAGTGAGGA CAACTCCCTG
CAGGAACTGG ACCAGCCGGC TGCCCCAGTG GCGGTGACGT CAAACGCCTC CACCACTAAG
TACCCTCAGA GCCCGACTAA TTCCAAGGCC CAGAAGAAGA ACCGGAACAA ACTGCGCCGG
CAGCACTCCT ACGACACCTT CGTGGACCTG CAGAAGGAAG AAGCCGCCCT GGCCCCGCGC
AGCGTAAGCC TGAAAGACAA GGGCCGATTC ATGGATGGGA GCCCCTACGC CCACATGTTT
GAGATGTCAG CTGGCGAGAG CACCTTTGCC AACAACAAGT CCTCAGTGCC CACTGCCGGA
CATCACCACC ACAACAACCC CGGCGGCGGG TACATGCTCA GCAAGTCGCT CTACCCTGAC
CGGGTCACGC AAAACCCTTT CATCCCCACT TTTGGGGACG ACCAGTGCTT GCTCCATGGC
AGCAAATCCT ACTTCTTCAG GCAGCCCACG GTGGCGGGGG CGTCGAAAGC CAGGCCGGAC
TTCCGGGCCC TTGTCACCAA CAAGCCGGTG GTCTCGGCCC TTCATGGGGC CGTGCCAGCC
CGTTTCCAGA AGGACATCTG TATAGGGAAC CAGTCCAACC CCTGTGTGCC TAACAACAAA
AACCCCAGGG CTTTCAATGG CTCCAGCAAT GGGCATGTTT ATGAGAAACT TTCTAGTATT
GAGTCTGATG TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011518931.1
CDS770..5224
Misc Feature(1)866..1948(+)
Misc Feature(2)1004..1390(+)
Misc Feature(3)1976..3178(+)
Misc Feature(4)2144..>2395(+)
Misc Feature(5)2303..3139(+)
Misc Feature(6)2303..2965(+)
Misc Feature(7)2438..3256(+)
Misc Feature(8)3287..5221(+)
Translation

Target ORF information:

RefSeq Version XM_011520629
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011520629

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGAAGCCCA GAGCGGAGTG CTGTTCTCCC AAGTTCTGGT TGGTGTTGGC CGTCCTGGCC 
GTGTCAGGCA GCAGAGCTCG TTCTCAGAAG AGCCCCCCCA GCATTGGCAT TGCTGTCATC
CTCGTGGGCA CTTCCGACGA GGTGGCCATC AAGGATGCCC ACGAGAAAGA TGATTTCCAC
CATCTCTCCG TGGTACCCCG GGTGGAACTG GTAGCCATGA ATGAGACCGA CCCAAAGAGC
ATCATCACCC GCATCTGTGA TCTCATGTCT GACCGGAAGA TCCAGGGGGT GGTGTTTGCT
GATGACACAG ACCAGGAAGC CATCGCCCAG ATCCTCGATT TCATTTCAGC ACAGACTCTC
ACCCCCATCC TGGGCATCCA CGGGGGCTCC TCTATGATAA TGGCAGATAA GGATGAATCC
TCCATGTTCT TCCAGTTTGG CCCATCAATT GAACAGCAAG CTTCCGTAAT GCTCAACATC
ATGGAAGAAT ATGACTGGTA CATCTTTTCT ATCGTCACCA CCTATTTCCC TGGCTACCAG
GACTTTGTAA ACAAGATCCG CAGCACCATT GAGAATAGCT TTGTGGGCTG GGAGCTAGAG
GAGGTCCTCC TACTGGACAT GTCCCTGGAC GATGGAGATT CTAAGATCCA GAATCAGCTC
AAGAAACTTC AAAGCCCCAT CATTCTTCTT TACTGTACCA AGGAAGAAGC CACCTACATC
TTTGAAGTGG CCAACTCAGT AGGGCTGACT GGCTATGGCT ACACGTGGAT CGTGCCCAGT
CTGGTGGCAG GGGATACAGA CACAGTGCCT GCGGAGTTCC CCACTGGGCT CATCTCTGTA
TCATATGATG AATGGGACTA TGGCCTCCCC GCCAGAGTGA GAGATGGAAT TGCCATAATC
ACCACTGCTG CTTCTGACAT GCTGTCTGAG CACAGCTTCA TCCCTGAGCC CAAAAGCAGT
TGTTACAACA CCCACGAGAA GAGAATCTAC CAGTCCAATA TGCTAAATAG GTATCTGATC
AATGTCACTT TTGAGGGGAG GAATTTGTCC TTCAGTGAAG ATGGCTACCA GATGCACCCG
AAACTGGTGA TAATTCTTCT GAACAAGGAG AGGAAGTGGG AAAGGGTGGG GAAGTGGAAA
GACAAGTCCC TGCAGATGAA GTACTATGTG TGGCCCCGAA TGTGTCCAGA GACTGAAGAG
CAGGAGGATG ACCATCTGAG CATTGTGACC CTGGAGGAGG CACCATTTGT CATTGTGGAA
AGTGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACAG TCCCCTGCCA AAAACGCATA
GTCACTGAGA ATAAAACAGA CGAGGAGCCG GGTTACATCA AAAAATGCTG CAAGGGGTTC
TGTATTGACA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA CCTTTACCTG
GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG
GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGCTCACTCA CCATCAATGA GGAACGATCG
GAGGTGGTCG ACTTCTCTGT GCCCTTCATA GAGACAGGCA TCAGTGTCAT GGTGTCACGC
AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTATGGGTG
ATGATGTTTG TGATGCTGCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT TGAGTACTTC
AGCCCTGTGG GTTATAACAG GTGCCTCGCT GATGGCAGAG AGCCTGGTGG ACCCTCTTTC
ACCATCGGCA AAGCTATTTG GTTGCTCTGG GGTCTGGTGT TTAACAACTC CGTACCTGTG
CAGAACCCAA AGGGGACCAC CTCCAAGATC ATGGTGTCAG TGTGGGCCTT CTTTGCTGTC
ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAATATGTG
GACCAGGTTT CTGGCCTGAG CGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
TTCCGCTTTG GGACCGTGCC CAACGGCAGC ACAGAGAGAA ATATTCGCAA TAACTATGCA
GAAATGCATG CCTACATGGG AAAGTTCAAC CAGAGGGGTG TAGATGATGC ATTGCTCTCC
CTGAAAACAG GGAAACTGGA TGCCTTCATC TATGATGCAG CAGTGCTGAA CTATATGGCA
GGCAGAGATG AAGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCTTCCACT
GGCTATGGCA TTGCCATCCA AAAAGATTCT GGGTGGAAGC GCCAGGTGGA CCTTGCTATC
CTGCAGCTCT TTGGAGATGG GGAGATGGAA GAACTGGAAG CTCTCTGGCT CACTGGCATT
TGTCACAATG AGAAGAATGA GGTCATGAGC AGCCAGCTGG ACATTGACAA CATGGCAGGG
GTCTTCTACA TGTTGGGGGC GGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGCGAACAC
CTTTTCTATT GGCAGTTCCG ACATTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG
GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GGGTGGCGAT CGAGGAGCGC
CAGTCTGTAA TGAACTCCCC CACCGCAACC ATGAACAACA CACACTCCAA CATCCTGCGC
CTGCTGCGCA CGGCCAAGAA CATGGCTAAC CTGTCTGGTG TGAATGGCTC ACCGCAGAGC
GCCCTGGACT TCATCCGACG GGAGTCATCC GTCTATGACA TCTCAGAGCA CCGCCGCAGC
TTCACGCATT CTGACTGCAA ATCCTACAAC AACCCGCCCT GTGAGGAGAA CCTCTTCAGT
GACTACATCA GTGAGGTAGA GAGAACGTTC GGGAACCTGC AGCTGAAGGA CAGCAACGTG
TACCAAGATC ACTACCACCA TCACCACCGG CCCCATAGTA TTGGCAGTGC CAGCTCCATC
GATGGGCTCT ACGACTGTGA CAACCCACCC TTCACCACCC AGTCCAGGTC CATCAGCAAG
AAGCCCCTGG ACATCGGCCT CCCCTCCTCC AAGCACAGCC AGCTCAGTGA CCTGTACGGC
AAATTCTCCT TCAAGAGCGA CCGCTACAGT GGCCACGACG ACTTGATCCG CTCCGATGTC
TCTGACATCT CAACCCACAC CGTCACCTAT GGGAACATCG AGGGCAATGC CGCCAAGAGG
CGTAAGCAGC AATATAAGGA CAGCCTGAAG AAGCGGCCTG CCTCGGCCAA GTCCCGCAGG
GAGTTTGACG AGATCGAGCT GGCCTACCGT CGCCGACCGC CCCGCTCCCC TGACCACAAG
CGCTACTTCA GGGACAAGGA AGGGCTACGG GACTTCTACC TGGACCAGTT CCGAACAAAG
GAGAACTCAC CCCACTGGGA GCACGTAGAC CTGACCGACA TCTACAAGGA GCGGAGTGAT
GACTTTAAGC GCGACTCCGT CAGCGGAGGA GGGCCCTGTA CCAACAGGTC TCACATCAAG
CACGGGACGG GCGACAAACA CGGCGTGGTC AGCGGGGTAC CTGCACCTTG GGAGAAGAAC
CTGACCAACG TGGAGTGGGA GGACCGGTCC GGGGGCAACT TCTGCCGCAG CTGTCCCTCC
AAGCTGCACA ACTACTCCAC GACGGTGACG GGTCAGAACT CGGGCAGGCA GGCGTGCATC
CGGTGTGAGG CTTGCAAGAA AGCAGGCAAC CTGTATGACA TCAGTGAGGA CAACTCCCTG
CAGGAACTGG ACCAGCCGGC TGCCCCAGTG GCGGTGACGT CAAACGCCTC CACCACTAAG
TACCCTCAGA GCCCGACTAA TTCCAAGGCC CAGAAGAAGA ACCGGAACAA ACTGCGCCGG
CAGCACTCCT ACGACACCTT CGTGGACCTG CAGAAGGAAG AAGCCGCCCT GGCCCCGCGC
AGCGTAAGCC TGAAAGACAA GGGCCGATTC ATGGATGGGA GCCCCTACGC CCACATGTTT
GAGATGTCAG CTGGCGAGAG CACCTTTGCC AACAACAAGT CCTCAGTGCC CACTGCCGGA
CATCACCACC ACAACAACCC CGGCGGCGGG TACATGCTCA GCAAGTCGCT CTACCCTGAC
CGGGTCACGC AAAACCCTTT CATCCCCACT TTTGGGGACG ACCAGTGCTT GCTCCATGGC
AGCAAATCCT ACTTCTTCAG GCAGCCCACG GTGGCGGGGG CGTCGAAAGC CAGGCCGGAC
TTCCGGGCCC TTGTCACCAA CAAGCCGGTG GTCTCGGCCC TTCATGGGGC CGTGCCAGCC
CGTTTCCAGA AGGACATCTG TATAGGGAAC CAGTCCAACC CCTGTGTGCC TAACAACAAA
AACCCCAGGG CTTTCAATGG CTCCAGCAAT GGGCATGTTT ATGAGAAACT TTCTAGTATT
GAGTCTGATG TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26128
Clone ID Related Accession (Same CDS sequence) NM_000834 , XM_011520628 , XM_011520629 , XM_011520630
Accession Version XM_011520630.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4455bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product glutamate receptor ionotropic, NMDA 2B isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009714.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGAAGCCCA GAGCGGAGTG CTGTTCTCCC AAGTTCTGGT TGGTGTTGGC CGTCCTGGCC 
GTGTCAGGCA GCAGAGCTCG TTCTCAGAAG AGCCCCCCCA GCATTGGCAT TGCTGTCATC
CTCGTGGGCA CTTCCGACGA GGTGGCCATC AAGGATGCCC ACGAGAAAGA TGATTTCCAC
CATCTCTCCG TGGTACCCCG GGTGGAACTG GTAGCCATGA ATGAGACCGA CCCAAAGAGC
ATCATCACCC GCATCTGTGA TCTCATGTCT GACCGGAAGA TCCAGGGGGT GGTGTTTGCT
GATGACACAG ACCAGGAAGC CATCGCCCAG ATCCTCGATT TCATTTCAGC ACAGACTCTC
ACCCCCATCC TGGGCATCCA CGGGGGCTCC TCTATGATAA TGGCAGATAA GGATGAATCC
TCCATGTTCT TCCAGTTTGG CCCATCAATT GAACAGCAAG CTTCCGTAAT GCTCAACATC
ATGGAAGAAT ATGACTGGTA CATCTTTTCT ATCGTCACCA CCTATTTCCC TGGCTACCAG
GACTTTGTAA ACAAGATCCG CAGCACCATT GAGAATAGCT TTGTGGGCTG GGAGCTAGAG
GAGGTCCTCC TACTGGACAT GTCCCTGGAC GATGGAGATT CTAAGATCCA GAATCAGCTC
AAGAAACTTC AAAGCCCCAT CATTCTTCTT TACTGTACCA AGGAAGAAGC CACCTACATC
TTTGAAGTGG CCAACTCAGT AGGGCTGACT GGCTATGGCT ACACGTGGAT CGTGCCCAGT
CTGGTGGCAG GGGATACAGA CACAGTGCCT GCGGAGTTCC CCACTGGGCT CATCTCTGTA
TCATATGATG AATGGGACTA TGGCCTCCCC GCCAGAGTGA GAGATGGAAT TGCCATAATC
ACCACTGCTG CTTCTGACAT GCTGTCTGAG CACAGCTTCA TCCCTGAGCC CAAAAGCAGT
TGTTACAACA CCCACGAGAA GAGAATCTAC CAGTCCAATA TGCTAAATAG GTATCTGATC
AATGTCACTT TTGAGGGGAG GAATTTGTCC TTCAGTGAAG ATGGCTACCA GATGCACCCG
AAACTGGTGA TAATTCTTCT GAACAAGGAG AGGAAGTGGG AAAGGGTGGG GAAGTGGAAA
GACAAGTCCC TGCAGATGAA GTACTATGTG TGGCCCCGAA TGTGTCCAGA GACTGAAGAG
CAGGAGGATG ACCATCTGAG CATTGTGACC CTGGAGGAGG CACCATTTGT CATTGTGGAA
AGTGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACAG TCCCCTGCCA AAAACGCATA
GTCACTGAGA ATAAAACAGA CGAGGAGCCG GGTTACATCA AAAAATGCTG CAAGGGGTTC
TGTATTGACA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA CCTTTACCTG
GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG
GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGCTCACTCA CCATCAATGA GGAACGATCG
GAGGTGGTCG ACTTCTCTGT GCCCTTCATA GAGACAGGCA TCAGTGTCAT GGTGTCACGC
AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTATGGGTG
ATGATGTTTG TGATGCTGCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT TGAGTACTTC
AGCCCTGTGG GTTATAACAG GTGCCTCGCT GATGGCAGAG AGCCTGGTGG ACCCTCTTTC
ACCATCGGCA AAGCTATTTG GTTGCTCTGG GGTCTGGTGT TTAACAACTC CGTACCTGTG
CAGAACCCAA AGGGGACCAC CTCCAAGATC ATGGTGTCAG TGTGGGCCTT CTTTGCTGTC
ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAATATGTG
GACCAGGTTT CTGGCCTGAG CGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
TTCCGCTTTG GGACCGTGCC CAACGGCAGC ACAGAGAGAA ATATTCGCAA TAACTATGCA
GAAATGCATG CCTACATGGG AAAGTTCAAC CAGAGGGGTG TAGATGATGC ATTGCTCTCC
CTGAAAACAG GGAAACTGGA TGCCTTCATC TATGATGCAG CAGTGCTGAA CTATATGGCA
GGCAGAGATG AAGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCTTCCACT
GGCTATGGCA TTGCCATCCA AAAAGATTCT GGGTGGAAGC GCCAGGTGGA CCTTGCTATC
CTGCAGCTCT TTGGAGATGG GGAGATGGAA GAACTGGAAG CTCTCTGGCT CACTGGCATT
TGTCACAATG AGAAGAATGA GGTCATGAGC AGCCAGCTGG ACATTGACAA CATGGCAGGG
GTCTTCTACA TGTTGGGGGC GGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGCGAACAC
CTTTTCTATT GGCAGTTCCG ACATTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG
GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GGGTGGCGAT CGAGGAGCGC
CAGTCTGTAA TGAACTCCCC CACCGCAACC ATGAACAACA CACACTCCAA CATCCTGCGC
CTGCTGCGCA CGGCCAAGAA CATGGCTAAC CTGTCTGGTG TGAATGGCTC ACCGCAGAGC
GCCCTGGACT TCATCCGACG GGAGTCATCC GTCTATGACA TCTCAGAGCA CCGCCGCAGC
TTCACGCATT CTGACTGCAA ATCCTACAAC AACCCGCCCT GTGAGGAGAA CCTCTTCAGT
GACTACATCA GTGAGGTAGA GAGAACGTTC GGGAACCTGC AGCTGAAGGA CAGCAACGTG
TACCAAGATC ACTACCACCA TCACCACCGG CCCCATAGTA TTGGCAGTGC CAGCTCCATC
GATGGGCTCT ACGACTGTGA CAACCCACCC TTCACCACCC AGTCCAGGTC CATCAGCAAG
AAGCCCCTGG ACATCGGCCT CCCCTCCTCC AAGCACAGCC AGCTCAGTGA CCTGTACGGC
AAATTCTCCT TCAAGAGCGA CCGCTACAGT GGCCACGACG ACTTGATCCG CTCCGATGTC
TCTGACATCT CAACCCACAC CGTCACCTAT GGGAACATCG AGGGCAATGC CGCCAAGAGG
CGTAAGCAGC AATATAAGGA CAGCCTGAAG AAGCGGCCTG CCTCGGCCAA GTCCCGCAGG
GAGTTTGACG AGATCGAGCT GGCCTACCGT CGCCGACCGC CCCGCTCCCC TGACCACAAG
CGCTACTTCA GGGACAAGGA AGGGCTACGG GACTTCTACC TGGACCAGTT CCGAACAAAG
GAGAACTCAC CCCACTGGGA GCACGTAGAC CTGACCGACA TCTACAAGGA GCGGAGTGAT
GACTTTAAGC GCGACTCCGT CAGCGGAGGA GGGCCCTGTA CCAACAGGTC TCACATCAAG
CACGGGACGG GCGACAAACA CGGCGTGGTC AGCGGGGTAC CTGCACCTTG GGAGAAGAAC
CTGACCAACG TGGAGTGGGA GGACCGGTCC GGGGGCAACT TCTGCCGCAG CTGTCCCTCC
AAGCTGCACA ACTACTCCAC GACGGTGACG GGTCAGAACT CGGGCAGGCA GGCGTGCATC
CGGTGTGAGG CTTGCAAGAA AGCAGGCAAC CTGTATGACA TCAGTGAGGA CAACTCCCTG
CAGGAACTGG ACCAGCCGGC TGCCCCAGTG GCGGTGACGT CAAACGCCTC CACCACTAAG
TACCCTCAGA GCCCGACTAA TTCCAAGGCC CAGAAGAAGA ACCGGAACAA ACTGCGCCGG
CAGCACTCCT ACGACACCTT CGTGGACCTG CAGAAGGAAG AAGCCGCCCT GGCCCCGCGC
AGCGTAAGCC TGAAAGACAA GGGCCGATTC ATGGATGGGA GCCCCTACGC CCACATGTTT
GAGATGTCAG CTGGCGAGAG CACCTTTGCC AACAACAAGT CCTCAGTGCC CACTGCCGGA
CATCACCACC ACAACAACCC CGGCGGCGGG TACATGCTCA GCAAGTCGCT CTACCCTGAC
CGGGTCACGC AAAACCCTTT CATCCCCACT TTTGGGGACG ACCAGTGCTT GCTCCATGGC
AGCAAATCCT ACTTCTTCAG GCAGCCCACG GTGGCGGGGG CGTCGAAAGC CAGGCCGGAC
TTCCGGGCCC TTGTCACCAA CAAGCCGGTG GTCTCGGCCC TTCATGGGGC CGTGCCAGCC
CGTTTCCAGA AGGACATCTG TATAGGGAAC CAGTCCAACC CCTGTGTGCC TAACAACAAA
AACCCCAGGG CTTTCAATGG CTCCAGCAAT GGGCATGTTT ATGAGAAACT TTCTAGTATT
GAGTCTGATG TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011518932.1
CDS538..4992
Misc Feature(1)634..1716(+)
Misc Feature(2)772..1158(+)
Misc Feature(3)1744..2946(+)
Misc Feature(4)1912..>2163(+)
Misc Feature(5)2071..2907(+)
Misc Feature(6)2071..2733(+)
Misc Feature(7)2206..3024(+)
Misc Feature(8)3055..4989(+)
Translation

Target ORF information:

RefSeq Version XM_011520630
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011520630

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGAAGCCCA GAGCGGAGTG CTGTTCTCCC AAGTTCTGGT TGGTGTTGGC CGTCCTGGCC 
GTGTCAGGCA GCAGAGCTCG TTCTCAGAAG AGCCCCCCCA GCATTGGCAT TGCTGTCATC
CTCGTGGGCA CTTCCGACGA GGTGGCCATC AAGGATGCCC ACGAGAAAGA TGATTTCCAC
CATCTCTCCG TGGTACCCCG GGTGGAACTG GTAGCCATGA ATGAGACCGA CCCAAAGAGC
ATCATCACCC GCATCTGTGA TCTCATGTCT GACCGGAAGA TCCAGGGGGT GGTGTTTGCT
GATGACACAG ACCAGGAAGC CATCGCCCAG ATCCTCGATT TCATTTCAGC ACAGACTCTC
ACCCCCATCC TGGGCATCCA CGGGGGCTCC TCTATGATAA TGGCAGATAA GGATGAATCC
TCCATGTTCT TCCAGTTTGG CCCATCAATT GAACAGCAAG CTTCCGTAAT GCTCAACATC
ATGGAAGAAT ATGACTGGTA CATCTTTTCT ATCGTCACCA CCTATTTCCC TGGCTACCAG
GACTTTGTAA ACAAGATCCG CAGCACCATT GAGAATAGCT TTGTGGGCTG GGAGCTAGAG
GAGGTCCTCC TACTGGACAT GTCCCTGGAC GATGGAGATT CTAAGATCCA GAATCAGCTC
AAGAAACTTC AAAGCCCCAT CATTCTTCTT TACTGTACCA AGGAAGAAGC CACCTACATC
TTTGAAGTGG CCAACTCAGT AGGGCTGACT GGCTATGGCT ACACGTGGAT CGTGCCCAGT
CTGGTGGCAG GGGATACAGA CACAGTGCCT GCGGAGTTCC CCACTGGGCT CATCTCTGTA
TCATATGATG AATGGGACTA TGGCCTCCCC GCCAGAGTGA GAGATGGAAT TGCCATAATC
ACCACTGCTG CTTCTGACAT GCTGTCTGAG CACAGCTTCA TCCCTGAGCC CAAAAGCAGT
TGTTACAACA CCCACGAGAA GAGAATCTAC CAGTCCAATA TGCTAAATAG GTATCTGATC
AATGTCACTT TTGAGGGGAG GAATTTGTCC TTCAGTGAAG ATGGCTACCA GATGCACCCG
AAACTGGTGA TAATTCTTCT GAACAAGGAG AGGAAGTGGG AAAGGGTGGG GAAGTGGAAA
GACAAGTCCC TGCAGATGAA GTACTATGTG TGGCCCCGAA TGTGTCCAGA GACTGAAGAG
CAGGAGGATG ACCATCTGAG CATTGTGACC CTGGAGGAGG CACCATTTGT CATTGTGGAA
AGTGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACAG TCCCCTGCCA AAAACGCATA
GTCACTGAGA ATAAAACAGA CGAGGAGCCG GGTTACATCA AAAAATGCTG CAAGGGGTTC
TGTATTGACA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA CCTTTACCTG
GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG
GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGCTCACTCA CCATCAATGA GGAACGATCG
GAGGTGGTCG ACTTCTCTGT GCCCTTCATA GAGACAGGCA TCAGTGTCAT GGTGTCACGC
AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTATGGGTG
ATGATGTTTG TGATGCTGCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT TGAGTACTTC
AGCCCTGTGG GTTATAACAG GTGCCTCGCT GATGGCAGAG AGCCTGGTGG ACCCTCTTTC
ACCATCGGCA AAGCTATTTG GTTGCTCTGG GGTCTGGTGT TTAACAACTC CGTACCTGTG
CAGAACCCAA AGGGGACCAC CTCCAAGATC ATGGTGTCAG TGTGGGCCTT CTTTGCTGTC
ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAATATGTG
GACCAGGTTT CTGGCCTGAG CGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
TTCCGCTTTG GGACCGTGCC CAACGGCAGC ACAGAGAGAA ATATTCGCAA TAACTATGCA
GAAATGCATG CCTACATGGG AAAGTTCAAC CAGAGGGGTG TAGATGATGC ATTGCTCTCC
CTGAAAACAG GGAAACTGGA TGCCTTCATC TATGATGCAG CAGTGCTGAA CTATATGGCA
GGCAGAGATG AAGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCTTCCACT
GGCTATGGCA TTGCCATCCA AAAAGATTCT GGGTGGAAGC GCCAGGTGGA CCTTGCTATC
CTGCAGCTCT TTGGAGATGG GGAGATGGAA GAACTGGAAG CTCTCTGGCT CACTGGCATT
TGTCACAATG AGAAGAATGA GGTCATGAGC AGCCAGCTGG ACATTGACAA CATGGCAGGG
GTCTTCTACA TGTTGGGGGC GGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGCGAACAC
CTTTTCTATT GGCAGTTCCG ACATTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG
GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GGGTGGCGAT CGAGGAGCGC
CAGTCTGTAA TGAACTCCCC CACCGCAACC ATGAACAACA CACACTCCAA CATCCTGCGC
CTGCTGCGCA CGGCCAAGAA CATGGCTAAC CTGTCTGGTG TGAATGGCTC ACCGCAGAGC
GCCCTGGACT TCATCCGACG GGAGTCATCC GTCTATGACA TCTCAGAGCA CCGCCGCAGC
TTCACGCATT CTGACTGCAA ATCCTACAAC AACCCGCCCT GTGAGGAGAA CCTCTTCAGT
GACTACATCA GTGAGGTAGA GAGAACGTTC GGGAACCTGC AGCTGAAGGA CAGCAACGTG
TACCAAGATC ACTACCACCA TCACCACCGG CCCCATAGTA TTGGCAGTGC CAGCTCCATC
GATGGGCTCT ACGACTGTGA CAACCCACCC TTCACCACCC AGTCCAGGTC CATCAGCAAG
AAGCCCCTGG ACATCGGCCT CCCCTCCTCC AAGCACAGCC AGCTCAGTGA CCTGTACGGC
AAATTCTCCT TCAAGAGCGA CCGCTACAGT GGCCACGACG ACTTGATCCG CTCCGATGTC
TCTGACATCT CAACCCACAC CGTCACCTAT GGGAACATCG AGGGCAATGC CGCCAAGAGG
CGTAAGCAGC AATATAAGGA CAGCCTGAAG AAGCGGCCTG CCTCGGCCAA GTCCCGCAGG
GAGTTTGACG AGATCGAGCT GGCCTACCGT CGCCGACCGC CCCGCTCCCC TGACCACAAG
CGCTACTTCA GGGACAAGGA AGGGCTACGG GACTTCTACC TGGACCAGTT CCGAACAAAG
GAGAACTCAC CCCACTGGGA GCACGTAGAC CTGACCGACA TCTACAAGGA GCGGAGTGAT
GACTTTAAGC GCGACTCCGT CAGCGGAGGA GGGCCCTGTA CCAACAGGTC TCACATCAAG
CACGGGACGG GCGACAAACA CGGCGTGGTC AGCGGGGTAC CTGCACCTTG GGAGAAGAAC
CTGACCAACG TGGAGTGGGA GGACCGGTCC GGGGGCAACT TCTGCCGCAG CTGTCCCTCC
AAGCTGCACA ACTACTCCAC GACGGTGACG GGTCAGAACT CGGGCAGGCA GGCGTGCATC
CGGTGTGAGG CTTGCAAGAA AGCAGGCAAC CTGTATGACA TCAGTGAGGA CAACTCCCTG
CAGGAACTGG ACCAGCCGGC TGCCCCAGTG GCGGTGACGT CAAACGCCTC CACCACTAAG
TACCCTCAGA GCCCGACTAA TTCCAAGGCC CAGAAGAAGA ACCGGAACAA ACTGCGCCGG
CAGCACTCCT ACGACACCTT CGTGGACCTG CAGAAGGAAG AAGCCGCCCT GGCCCCGCGC
AGCGTAAGCC TGAAAGACAA GGGCCGATTC ATGGATGGGA GCCCCTACGC CCACATGTTT
GAGATGTCAG CTGGCGAGAG CACCTTTGCC AACAACAAGT CCTCAGTGCC CACTGCCGGA
CATCACCACC ACAACAACCC CGGCGGCGGG TACATGCTCA GCAAGTCGCT CTACCCTGAC
CGGGTCACGC AAAACCCTTT CATCCCCACT TTTGGGGACG ACCAGTGCTT GCTCCATGGC
AGCAAATCCT ACTTCTTCAG GCAGCCCACG GTGGCGGGGG CGTCGAAAGC CAGGCCGGAC
TTCCGGGCCC TTGTCACCAA CAAGCCGGTG GTCTCGGCCC TTCATGGGGC CGTGCCAGCC
CGTTTCCAGA AGGACATCTG TATAGGGAAC CAGTCCAACC CCTGTGTGCC TAACAACAAA
AACCCCAGGG CTTTCAATGG CTCCAGCAAT GGGCATGTTT ATGAGAAACT TTCTAGTATT
GAGTCTGATG TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu46089
Clone ID Related Accession (Same CDS sequence) XM_005253351
Accession Version XM_005253351.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2241bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product glutamate receptor ionotropic, NMDA 2B isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009714.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530399103. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCAGGCA GAGATGAAGG CTGCAAGCTG GTGACCATTG GCAGTGGGAA GGTCTTTGCT 
TCCACTGGCT ATGGCATTGC CATCCAAAAA GATTCTGGGT GGAAGCGCCA GGTGGACCTT
GCTATCCTGC AGCTCTTTGG AGATGGGGAG ATGGAAGAAC TGGAAGCTCT CTGGCTCACT
GGCATTTGTC ACAATGAGAA GAATGAGGTC ATGAGCAGCC AGCTGGACAT TGACAACATG
GCAGGGGTCT TCTACATGTT GGGGGCGGCC ATGGCTCTCA GCCTCATCAC CTTCATCTGC
GAACACCTTT TCTATTGGCA GTTCCGACAT TGCTTTATGG GTGTCTGTTC TGGCAAGCCT
GGCATGGTCT TCTCCATCAG CAGAGGTATC TACAGCTGCA TCCATGGGGT GGCGATCGAG
GAGCGCCAGT CTGTAATGAA CTCCCCCACC GCAACCATGA ACAACACACA CTCCAACATC
CTGCGCCTGC TGCGCACGGC CAAGAACATG GCTAACCTGT CTGGTGTGAA TGGCTCACCG
CAGAGCGCCC TGGACTTCAT CCGACGGGAG TCATCCGTCT ATGACATCTC AGAGCACCGC
CGCAGCTTCA CGCATTCTGA CTGCAAATCC TACAACAACC CGCCCTGTGA GGAGAACCTC
TTCAGTGACT ACATCAGTGA GGTAGAGAGA ACGTTCGGGA ACCTGCAGCT GAAGGACAGC
AACGTGTACC AAGATCACTA CCACCATCAC CACCGGCCCC ATAGTATTGG CAGTGCCAGC
TCCATCGATG GGCTCTACGA CTGTGACAAC CCACCCTTCA CCACCCAGTC CAGGTCCATC
AGCAAGAAGC CCCTGGACAT CGGCCTCCCC TCCTCCAAGC ACAGCCAGCT CAGTGACCTG
TACGGCAAAT TCTCCTTCAA GAGCGACCGC TACAGTGGCC ACGACGACTT GATCCGCTCC
GATGTCTCTG ACATCTCAAC CCACACCGTC ACCTATGGGA ACATCGAGGG CAATGCCGCC
AAGAGGCGTA AGCAGCAATA TAAGGACAGC CTGAAGAAGC GGCCTGCCTC GGCCAAGTCC
CGCAGGGAGT TTGACGAGAT CGAGCTGGCC TACCGTCGCC GACCGCCCCG CTCCCCTGAC
CACAAGCGCT ACTTCAGGGA CAAGGAAGGG CTACGGGACT TCTACCTGGA CCAGTTCCGA
ACAAAGGAGA ACTCACCCCA CTGGGAGCAC GTAGACCTGA CCGACATCTA CAAGGAGCGG
AGTGATGACT TTAAGCGCGA CTCCGTCAGC GGAGGAGGGC CCTGTACCAA CAGGTCTCAC
ATCAAGCACG GGACGGGCGA CAAACACGGC GTGGTCAGCG GGGTACCTGC ACCTTGGGAG
AAGAACCTGA CCAACGTGGA GTGGGAGGAC CGGTCCGGGG GCAACTTCTG CCGCAGCTGT
CCCTCCAAGC TGCACAACTA CTCCACGACG GTGACGGGTC AGAACTCGGG CAGGCAGGCG
TGCATCCGGT GTGAGGCTTG CAAGAAAGCA GGCAACCTGT ATGACATCAG TGAGGACAAC
TCCCTGCAGG AACTGGACCA GCCGGCTGCC CCAGTGGCGG TGACGTCAAA CGCCTCCACC
ACTAAGTACC CTCAGAGCCC GACTAATTCC AAGGCCCAGA AGAAGAACCG GAACAAACTG
CGCCGGCAGC ACTCCTACGA CACCTTCGTG GACCTGCAGA AGGAAGAAGC CGCCCTGGCC
CCGCGCAGCG TAAGCCTGAA AGACAAGGGC CGATTCATGG ATGGGAGCCC CTACGCCCAC
ATGTTTGAGA TGTCAGCTGG CGAGAGCACC TTTGCCAACA ACAAGTCCTC AGTGCCCACT
GCCGGACATC ACCACCACAA CAACCCCGGC GGCGGGTACA TGCTCAGCAA GTCGCTCTAC
CCTGACCGGG TCACGCAAAA CCCTTTCATC CCCACTTTTG GGGACGACCA GTGCTTGCTC
CATGGCAGCA AATCCTACTT CTTCAGGCAG CCCACGGTGG CGGGGGCGTC GAAAGCCAGG
CCGGACTTCC GGGCCCTTGT CACCAACAAG CCGGTGGTCT CGGCCCTTCA TGGGGCCGTG
CCAGCCCGTT TCCAGAAGGA CATCTGTATA GGGAACCAGT CCAACCCCTG TGTGCCTAAC
AACAAAAACC CCAGGGCTTT CAATGGCTCC AGCAATGGGC ATGTTTATGA GAAACTTTCT
AGTATTGAGT CTGATGTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005253408.1
CDS116..2356
Misc Feature(1)116..310(+)
Misc Feature(2)419..2353(+)
Translation

Target ORF information:

RefSeq Version XM_005253351
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005253351

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCAGGCA GAGATGAAGG CTGCAAGCTG GTGACCATTG GCAGTGGGAA GGTCTTTGCT 
TCCACTGGCT ATGGCATTGC CATCCAAAAA GATTCTGGGT GGAAGCGCCA GGTGGACCTT
GCTATCCTGC AGCTCTTTGG AGATGGGGAG ATGGAAGAAC TGGAAGCTCT CTGGCTCACT
GGCATTTGTC ACAATGAGAA GAATGAGGTC ATGAGCAGCC AGCTGGACAT TGACAACATG
GCAGGGGTCT TCTACATGTT GGGGGCGGCC ATGGCTCTCA GCCTCATCAC CTTCATCTGC
GAACACCTTT TCTATTGGCA GTTCCGACAT TGCTTTATGG GTGTCTGTTC TGGCAAGCCT
GGCATGGTCT TCTCCATCAG CAGAGGTATC TACAGCTGCA TCCATGGGGT GGCGATCGAG
GAGCGCCAGT CTGTAATGAA CTCCCCCACC GCAACCATGA ACAACACACA CTCCAACATC
CTGCGCCTGC TGCGCACGGC CAAGAACATG GCTAACCTGT CTGGTGTGAA TGGCTCACCG
CAGAGCGCCC TGGACTTCAT CCGACGGGAG TCATCCGTCT ATGACATCTC AGAGCACCGC
CGCAGCTTCA CGCATTCTGA CTGCAAATCC TACAACAACC CGCCCTGTGA GGAGAACCTC
TTCAGTGACT ACATCAGTGA GGTAGAGAGA ACGTTCGGGA ACCTGCAGCT GAAGGACAGC
AACGTGTACC AAGATCACTA CCACCATCAC CACCGGCCCC ATAGTATTGG CAGTGCCAGC
TCCATCGATG GGCTCTACGA CTGTGACAAC CCACCCTTCA CCACCCAGTC CAGGTCCATC
AGCAAGAAGC CCCTGGACAT CGGCCTCCCC TCCTCCAAGC ACAGCCAGCT CAGTGACCTG
TACGGCAAAT TCTCCTTCAA GAGCGACCGC TACAGTGGCC ACGACGACTT GATCCGCTCC
GATGTCTCTG ACATCTCAAC CCACACCGTC ACCTATGGGA ACATCGAGGG CAATGCCGCC
AAGAGGCGTA AGCAGCAATA TAAGGACAGC CTGAAGAAGC GGCCTGCCTC GGCCAAGTCC
CGCAGGGAGT TTGACGAGAT CGAGCTGGCC TACCGTCGCC GACCGCCCCG CTCCCCTGAC
CACAAGCGCT ACTTCAGGGA CAAGGAAGGG CTACGGGACT TCTACCTGGA CCAGTTCCGA
ACAAAGGAGA ACTCACCCCA CTGGGAGCAC GTAGACCTGA CCGACATCTA CAAGGAGCGG
AGTGATGACT TTAAGCGCGA CTCCGTCAGC GGAGGAGGGC CCTGTACCAA CAGGTCTCAC
ATCAAGCACG GGACGGGCGA CAAACACGGC GTGGTCAGCG GGGTACCTGC ACCTTGGGAG
AAGAACCTGA CCAACGTGGA GTGGGAGGAC CGGTCCGGGG GCAACTTCTG CCGCAGCTGT
CCCTCCAAGC TGCACAACTA CTCCACGACG GTGACGGGTC AGAACTCGGG CAGGCAGGCG
TGCATCCGGT GTGAGGCTTG CAAGAAAGCA GGCAACCTGT ATGACATCAG TGAGGACAAC
TCCCTGCAGG AACTGGACCA GCCGGCTGCC CCAGTGGCGG TGACGTCAAA CGCCTCCACC
ACTAAGTACC CTCAGAGCCC GACTAATTCC AAGGCCCAGA AGAAGAACCG GAACAAACTG
CGCCGGCAGC ACTCCTACGA CACCTTCGTG GACCTGCAGA AGGAAGAAGC CGCCCTGGCC
CCGCGCAGCG TAAGCCTGAA AGACAAGGGC CGATTCATGG ATGGGAGCCC CTACGCCCAC
ATGTTTGAGA TGTCAGCTGG CGAGAGCACC TTTGCCAACA ACAAGTCCTC AGTGCCCACT
GCCGGACATC ACCACCACAA CAACCCCGGC GGCGGGTACA TGCTCAGCAA GTCGCTCTAC
CCTGACCGGG TCACGCAAAA CCCTTTCATC CCCACTTTTG GGGACGACCA GTGCTTGCTC
CATGGCAGCA AATCCTACTT CTTCAGGCAG CCCACGGTGG CGGGGGCGTC GAAAGCCAGG
CCGGACTTCC GGGCCCTTGT CACCAACAAG CCGGTGGTCT CGGCCCTTCA TGGGGCCGTG
CCAGCCCGTT TCCAGAAGGA CATCTGTATA GGGAACCAGT CCAACCCCTG TGTGCCTAAC
AACAAAAACC CCAGGGCTTT CAATGGCTCC AGCAATGGGC ATGTTTATGA GAAACTTTCT
AGTATTGAGT CTGATGTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26128
Clone ID Related Accession (Same CDS sequence) NM_000834 , XM_011520628 , XM_011520629 , XM_011520630
Accession Version NM_000834.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4455bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glutamate receptor ionotropic, NMDA 2B precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U90278.1, U88963.1 and AC007535.3. This sequence is a reference standard in the RefSeqGene project. On Feb 6, 2008 this sequence version replaced gi:6006003. Sequence Note: This RefSeq was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U11287.1, U90278.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGAAGCCCA GAGCGGAGTG CTGTTCTCCC AAGTTCTGGT TGGTGTTGGC CGTCCTGGCC 
GTGTCAGGCA GCAGAGCTCG TTCTCAGAAG AGCCCCCCCA GCATTGGCAT TGCTGTCATC
CTCGTGGGCA CTTCCGACGA GGTGGCCATC AAGGATGCCC ACGAGAAAGA TGATTTCCAC
CATCTCTCCG TGGTACCCCG GGTGGAACTG GTAGCCATGA ATGAGACCGA CCCAAAGAGC
ATCATCACCC GCATCTGTGA TCTCATGTCT GACCGGAAGA TCCAGGGGGT GGTGTTTGCT
GATGACACAG ACCAGGAAGC CATCGCCCAG ATCCTCGATT TCATTTCAGC ACAGACTCTC
ACCCCCATCC TGGGCATCCA CGGGGGCTCC TCTATGATAA TGGCAGATAA GGATGAATCC
TCCATGTTCT TCCAGTTTGG CCCATCAATT GAACAGCAAG CTTCCGTAAT GCTCAACATC
ATGGAAGAAT ATGACTGGTA CATCTTTTCT ATCGTCACCA CCTATTTCCC TGGCTACCAG
GACTTTGTAA ACAAGATCCG CAGCACCATT GAGAATAGCT TTGTGGGCTG GGAGCTAGAG
GAGGTCCTCC TACTGGACAT GTCCCTGGAC GATGGAGATT CTAAGATCCA GAATCAGCTC
AAGAAACTTC AAAGCCCCAT CATTCTTCTT TACTGTACCA AGGAAGAAGC CACCTACATC
TTTGAAGTGG CCAACTCAGT AGGGCTGACT GGCTATGGCT ACACGTGGAT CGTGCCCAGT
CTGGTGGCAG GGGATACAGA CACAGTGCCT GCGGAGTTCC CCACTGGGCT CATCTCTGTA
TCATATGATG AATGGGACTA TGGCCTCCCC GCCAGAGTGA GAGATGGAAT TGCCATAATC
ACCACTGCTG CTTCTGACAT GCTGTCTGAG CACAGCTTCA TCCCTGAGCC CAAAAGCAGT
TGTTACAACA CCCACGAGAA GAGAATCTAC CAGTCCAATA TGCTAAATAG GTATCTGATC
AATGTCACTT TTGAGGGGAG GAATTTGTCC TTCAGTGAAG ATGGCTACCA GATGCACCCG
AAACTGGTGA TAATTCTTCT GAACAAGGAG AGGAAGTGGG AAAGGGTGGG GAAGTGGAAA
GACAAGTCCC TGCAGATGAA GTACTATGTG TGGCCCCGAA TGTGTCCAGA GACTGAAGAG
CAGGAGGATG ACCATCTGAG CATTGTGACC CTGGAGGAGG CACCATTTGT CATTGTGGAA
AGTGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACAG TCCCCTGCCA AAAACGCATA
GTCACTGAGA ATAAAACAGA CGAGGAGCCG GGTTACATCA AAAAATGCTG CAAGGGGTTC
TGTATTGACA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA CCTTTACCTG
GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG
GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGCTCACTCA CCATCAATGA GGAACGATCG
GAGGTGGTCG ACTTCTCTGT GCCCTTCATA GAGACAGGCA TCAGTGTCAT GGTGTCACGC
AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTATGGGTG
ATGATGTTTG TGATGCTGCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT TGAGTACTTC
AGCCCTGTGG GTTATAACAG GTGCCTCGCT GATGGCAGAG AGCCTGGTGG ACCCTCTTTC
ACCATCGGCA AAGCTATTTG GTTGCTCTGG GGTCTGGTGT TTAACAACTC CGTACCTGTG
CAGAACCCAA AGGGGACCAC CTCCAAGATC ATGGTGTCAG TGTGGGCCTT CTTTGCTGTC
ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAATATGTG
GACCAGGTTT CTGGCCTGAG CGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
TTCCGCTTTG GGACCGTGCC CAACGGCAGC ACAGAGAGAA ATATTCGCAA TAACTATGCA
GAAATGCATG CCTACATGGG AAAGTTCAAC CAGAGGGGTG TAGATGATGC ATTGCTCTCC
CTGAAAACAG GGAAACTGGA TGCCTTCATC TATGATGCAG CAGTGCTGAA CTATATGGCA
GGCAGAGATG AAGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCTTCCACT
GGCTATGGCA TTGCCATCCA AAAAGATTCT GGGTGGAAGC GCCAGGTGGA CCTTGCTATC
CTGCAGCTCT TTGGAGATGG GGAGATGGAA GAACTGGAAG CTCTCTGGCT CACTGGCATT
TGTCACAATG AGAAGAATGA GGTCATGAGC AGCCAGCTGG ACATTGACAA CATGGCAGGG
GTCTTCTACA TGTTGGGGGC GGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGCGAACAC
CTTTTCTATT GGCAGTTCCG ACATTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG
GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GGGTGGCGAT CGAGGAGCGC
CAGTCTGTAA TGAACTCCCC CACCGCAACC ATGAACAACA CACACTCCAA CATCCTGCGC
CTGCTGCGCA CGGCCAAGAA CATGGCTAAC CTGTCTGGTG TGAATGGCTC ACCGCAGAGC
GCCCTGGACT TCATCCGACG GGAGTCATCC GTCTATGACA TCTCAGAGCA CCGCCGCAGC
TTCACGCATT CTGACTGCAA ATCCTACAAC AACCCGCCCT GTGAGGAGAA CCTCTTCAGT
GACTACATCA GTGAGGTAGA GAGAACGTTC GGGAACCTGC AGCTGAAGGA CAGCAACGTG
TACCAAGATC ACTACCACCA TCACCACCGG CCCCATAGTA TTGGCAGTGC CAGCTCCATC
GATGGGCTCT ACGACTGTGA CAACCCACCC TTCACCACCC AGTCCAGGTC CATCAGCAAG
AAGCCCCTGG ACATCGGCCT CCCCTCCTCC AAGCACAGCC AGCTCAGTGA CCTGTACGGC
AAATTCTCCT TCAAGAGCGA CCGCTACAGT GGCCACGACG ACTTGATCCG CTCCGATGTC
TCTGACATCT CAACCCACAC CGTCACCTAT GGGAACATCG AGGGCAATGC CGCCAAGAGG
CGTAAGCAGC AATATAAGGA CAGCCTGAAG AAGCGGCCTG CCTCGGCCAA GTCCCGCAGG
GAGTTTGACG AGATCGAGCT GGCCTACCGT CGCCGACCGC CCCGCTCCCC TGACCACAAG
CGCTACTTCA GGGACAAGGA AGGGCTACGG GACTTCTACC TGGACCAGTT CCGAACAAAG
GAGAACTCAC CCCACTGGGA GCACGTAGAC CTGACCGACA TCTACAAGGA GCGGAGTGAT
GACTTTAAGC GCGACTCCGT CAGCGGAGGA GGGCCCTGTA CCAACAGGTC TCACATCAAG
CACGGGACGG GCGACAAACA CGGCGTGGTC AGCGGGGTAC CTGCACCTTG GGAGAAGAAC
CTGACCAACG TGGAGTGGGA GGACCGGTCC GGGGGCAACT TCTGCCGCAG CTGTCCCTCC
AAGCTGCACA ACTACTCCAC GACGGTGACG GGTCAGAACT CGGGCAGGCA GGCGTGCATC
CGGTGTGAGG CTTGCAAGAA AGCAGGCAAC CTGTATGACA TCAGTGAGGA CAACTCCCTG
CAGGAACTGG ACCAGCCGGC TGCCCCAGTG GCGGTGACGT CAAACGCCTC CACCACTAAG
TACCCTCAGA GCCCGACTAA TTCCAAGGCC CAGAAGAAGA ACCGGAACAA ACTGCGCCGG
CAGCACTCCT ACGACACCTT CGTGGACCTG CAGAAGGAAG AAGCCGCCCT GGCCCCGCGC
AGCGTAAGCC TGAAAGACAA GGGCCGATTC ATGGATGGGA GCCCCTACGC CCACATGTTT
GAGATGTCAG CTGGCGAGAG CACCTTTGCC AACAACAAGT CCTCAGTGCC CACTGCCGGA
CATCACCACC ACAACAACCC CGGCGGCGGG TACATGCTCA GCAAGTCGCT CTACCCTGAC
CGGGTCACGC AAAACCCTTT CATCCCCACT TTTGGGGACG ACCAGTGCTT GCTCCATGGC
AGCAAATCCT ACTTCTTCAG GCAGCCCACG GTGGCGGGGG CGTCGAAAGC CAGGCCGGAC
TTCCGGGCCC TTGTCACCAA CAAGCCGGTG GTCTCGGCCC TTCATGGGGC CGTGCCAGCC
CGTTTCCAGA AGGACATCTG TATAGGGAAC CAGTCCAACC CCTGTGTGCC TAACAACAAA
AACCCCAGGG CTTTCAATGG CTCCAGCAAT GGGCATGTTT ATGAGAAACT TTCTAGTATT
GAGTCTGATG TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000825.2
CDS180..4634
Misc Feature(1)123..125(+)
Misc Feature(2)276..1358(+)
Misc Feature(3)414..800(+)
Misc Feature(4)1386..2588(+)
Misc Feature(5)1554..>1805(+)
Misc Feature(6)1713..2549(+)
Misc Feature(7)1713..2375(+)
Misc Feature(8)1848..2666(+)
Misc Feature(9)1851..1913(+)
Misc Feature(10)2022..2024(+)
Misc Feature(11)2082..2144(+)
Misc Feature(12)2631..2693(+)
Misc Feature(13)2697..4631(+)
Misc Feature(14)3063..3065(+)
Misc Feature(15)3294..3296(+)
Misc Feature(16)3504..3506(+)
Misc Feature(17)3576..3578(+)
Misc Feature(18)3642..3644(+)
Misc Feature(19)4053..4091(+)
Misc Feature(20)4086..4088(+)
Misc Feature(21)4599..4601(+)
Misc Feature(22)4623..4631(+)
Exon (1)1..161
Gene:GRIN2B
Gene Synonym:
Exon (2)162..590
Gene:GRIN2B
Gene Synonym:
Exon (3)591..1189
Gene:GRIN2B
Gene Synonym:
Exon (4)1190..1304
Gene:GRIN2B
Gene Synonym:
Exon (5)1305..1507
Gene:GRIN2B
Gene Synonym:
Exon (6)1508..1679
Gene:GRIN2B
Gene Synonym:
Exon (7)1680..1833
Gene:GRIN2B
Gene Synonym:
Exon (8)1834..1959
Gene:GRIN2B
Gene Synonym:
Exon (9)1960..2189
Gene:GRIN2B
Gene Synonym:
Exon (10)2190..2350
Gene:GRIN2B
Gene Synonym:
Exon (11)2351..2538
Gene:GRIN2B
Gene Synonym:
Exon (12)2539..2777
Gene:GRIN2B
Gene Synonym:
Exon (13)2778..5941
Gene:GRIN2B
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000834
Organism Homo sapiens (human)
Definition Homo sapiens glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000834

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGAAGCCCA GAGCGGAGTG CTGTTCTCCC AAGTTCTGGT TGGTGTTGGC CGTCCTGGCC 
GTGTCAGGCA GCAGAGCTCG TTCTCAGAAG AGCCCCCCCA GCATTGGCAT TGCTGTCATC
CTCGTGGGCA CTTCCGACGA GGTGGCCATC AAGGATGCCC ACGAGAAAGA TGATTTCCAC
CATCTCTCCG TGGTACCCCG GGTGGAACTG GTAGCCATGA ATGAGACCGA CCCAAAGAGC
ATCATCACCC GCATCTGTGA TCTCATGTCT GACCGGAAGA TCCAGGGGGT GGTGTTTGCT
GATGACACAG ACCAGGAAGC CATCGCCCAG ATCCTCGATT TCATTTCAGC ACAGACTCTC
ACCCCCATCC TGGGCATCCA CGGGGGCTCC TCTATGATAA TGGCAGATAA GGATGAATCC
TCCATGTTCT TCCAGTTTGG CCCATCAATT GAACAGCAAG CTTCCGTAAT GCTCAACATC
ATGGAAGAAT ATGACTGGTA CATCTTTTCT ATCGTCACCA CCTATTTCCC TGGCTACCAG
GACTTTGTAA ACAAGATCCG CAGCACCATT GAGAATAGCT TTGTGGGCTG GGAGCTAGAG
GAGGTCCTCC TACTGGACAT GTCCCTGGAC GATGGAGATT CTAAGATCCA GAATCAGCTC
AAGAAACTTC AAAGCCCCAT CATTCTTCTT TACTGTACCA AGGAAGAAGC CACCTACATC
TTTGAAGTGG CCAACTCAGT AGGGCTGACT GGCTATGGCT ACACGTGGAT CGTGCCCAGT
CTGGTGGCAG GGGATACAGA CACAGTGCCT GCGGAGTTCC CCACTGGGCT CATCTCTGTA
TCATATGATG AATGGGACTA TGGCCTCCCC GCCAGAGTGA GAGATGGAAT TGCCATAATC
ACCACTGCTG CTTCTGACAT GCTGTCTGAG CACAGCTTCA TCCCTGAGCC CAAAAGCAGT
TGTTACAACA CCCACGAGAA GAGAATCTAC CAGTCCAATA TGCTAAATAG GTATCTGATC
AATGTCACTT TTGAGGGGAG GAATTTGTCC TTCAGTGAAG ATGGCTACCA GATGCACCCG
AAACTGGTGA TAATTCTTCT GAACAAGGAG AGGAAGTGGG AAAGGGTGGG GAAGTGGAAA
GACAAGTCCC TGCAGATGAA GTACTATGTG TGGCCCCGAA TGTGTCCAGA GACTGAAGAG
CAGGAGGATG ACCATCTGAG CATTGTGACC CTGGAGGAGG CACCATTTGT CATTGTGGAA
AGTGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACAG TCCCCTGCCA AAAACGCATA
GTCACTGAGA ATAAAACAGA CGAGGAGCCG GGTTACATCA AAAAATGCTG CAAGGGGTTC
TGTATTGACA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA CCTTTACCTG
GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG
GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGCTCACTCA CCATCAATGA GGAACGATCG
GAGGTGGTCG ACTTCTCTGT GCCCTTCATA GAGACAGGCA TCAGTGTCAT GGTGTCACGC
AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTATGGGTG
ATGATGTTTG TGATGCTGCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT TGAGTACTTC
AGCCCTGTGG GTTATAACAG GTGCCTCGCT GATGGCAGAG AGCCTGGTGG ACCCTCTTTC
ACCATCGGCA AAGCTATTTG GTTGCTCTGG GGTCTGGTGT TTAACAACTC CGTACCTGTG
CAGAACCCAA AGGGGACCAC CTCCAAGATC ATGGTGTCAG TGTGGGCCTT CTTTGCTGTC
ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAATATGTG
GACCAGGTTT CTGGCCTGAG CGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
TTCCGCTTTG GGACCGTGCC CAACGGCAGC ACAGAGAGAA ATATTCGCAA TAACTATGCA
GAAATGCATG CCTACATGGG AAAGTTCAAC CAGAGGGGTG TAGATGATGC ATTGCTCTCC
CTGAAAACAG GGAAACTGGA TGCCTTCATC TATGATGCAG CAGTGCTGAA CTATATGGCA
GGCAGAGATG AAGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCTTCCACT
GGCTATGGCA TTGCCATCCA AAAAGATTCT GGGTGGAAGC GCCAGGTGGA CCTTGCTATC
CTGCAGCTCT TTGGAGATGG GGAGATGGAA GAACTGGAAG CTCTCTGGCT CACTGGCATT
TGTCACAATG AGAAGAATGA GGTCATGAGC AGCCAGCTGG ACATTGACAA CATGGCAGGG
GTCTTCTACA TGTTGGGGGC GGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGCGAACAC
CTTTTCTATT GGCAGTTCCG ACATTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG
GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GGGTGGCGAT CGAGGAGCGC
CAGTCTGTAA TGAACTCCCC CACCGCAACC ATGAACAACA CACACTCCAA CATCCTGCGC
CTGCTGCGCA CGGCCAAGAA CATGGCTAAC CTGTCTGGTG TGAATGGCTC ACCGCAGAGC
GCCCTGGACT TCATCCGACG GGAGTCATCC GTCTATGACA TCTCAGAGCA CCGCCGCAGC
TTCACGCATT CTGACTGCAA ATCCTACAAC AACCCGCCCT GTGAGGAGAA CCTCTTCAGT
GACTACATCA GTGAGGTAGA GAGAACGTTC GGGAACCTGC AGCTGAAGGA CAGCAACGTG
TACCAAGATC ACTACCACCA TCACCACCGG CCCCATAGTA TTGGCAGTGC CAGCTCCATC
GATGGGCTCT ACGACTGTGA CAACCCACCC TTCACCACCC AGTCCAGGTC CATCAGCAAG
AAGCCCCTGG ACATCGGCCT CCCCTCCTCC AAGCACAGCC AGCTCAGTGA CCTGTACGGC
AAATTCTCCT TCAAGAGCGA CCGCTACAGT GGCCACGACG ACTTGATCCG CTCCGATGTC
TCTGACATCT CAACCCACAC CGTCACCTAT GGGAACATCG AGGGCAATGC CGCCAAGAGG
CGTAAGCAGC AATATAAGGA CAGCCTGAAG AAGCGGCCTG CCTCGGCCAA GTCCCGCAGG
GAGTTTGACG AGATCGAGCT GGCCTACCGT CGCCGACCGC CCCGCTCCCC TGACCACAAG
CGCTACTTCA GGGACAAGGA AGGGCTACGG GACTTCTACC TGGACCAGTT CCGAACAAAG
GAGAACTCAC CCCACTGGGA GCACGTAGAC CTGACCGACA TCTACAAGGA GCGGAGTGAT
GACTTTAAGC GCGACTCCGT CAGCGGAGGA GGGCCCTGTA CCAACAGGTC TCACATCAAG
CACGGGACGG GCGACAAACA CGGCGTGGTC AGCGGGGTAC CTGCACCTTG GGAGAAGAAC
CTGACCAACG TGGAGTGGGA GGACCGGTCC GGGGGCAACT TCTGCCGCAG CTGTCCCTCC
AAGCTGCACA ACTACTCCAC GACGGTGACG GGTCAGAACT CGGGCAGGCA GGCGTGCATC
CGGTGTGAGG CTTGCAAGAA AGCAGGCAAC CTGTATGACA TCAGTGAGGA CAACTCCCTG
CAGGAACTGG ACCAGCCGGC TGCCCCAGTG GCGGTGACGT CAAACGCCTC CACCACTAAG
TACCCTCAGA GCCCGACTAA TTCCAAGGCC CAGAAGAAGA ACCGGAACAA ACTGCGCCGG
CAGCACTCCT ACGACACCTT CGTGGACCTG CAGAAGGAAG AAGCCGCCCT GGCCCCGCGC
AGCGTAAGCC TGAAAGACAA GGGCCGATTC ATGGATGGGA GCCCCTACGC CCACATGTTT
GAGATGTCAG CTGGCGAGAG CACCTTTGCC AACAACAAGT CCTCAGTGCC CACTGCCGGA
CATCACCACC ACAACAACCC CGGCGGCGGG TACATGCTCA GCAAGTCGCT CTACCCTGAC
CGGGTCACGC AAAACCCTTT CATCCCCACT TTTGGGGACG ACCAGTGCTT GCTCCATGGC
AGCAAATCCT ACTTCTTCAG GCAGCCCACG GTGGCGGGGG CGTCGAAAGC CAGGCCGGAC
TTCCGGGCCC TTGTCACCAA CAAGCCGGTG GTCTCGGCCC TTCATGGGGC CGTGCCAGCC
CGTTTCCAGA AGGACATCTG TATAGGGAAC CAGTCCAACC CCTGTGTGCC TAACAACAAA
AACCCCAGGG CTTTCAATGG CTCCAGCAAT GGGCATGTTT ATGAGAAACT TTCTAGTATT
GAGTCTGATG TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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