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SLC25A4 cDNA ORF clone, Homo sapiens (human)

Gene Symbol SLC25A4
Entrez Gene ID 291
Full Name solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 4
Synonyms AAC1, ANT, ANT 1, ANT1, MTDPS12, PEO2, PEO3, T1
General protein information
Preferred Names
ADP/ATP translocase 1
Names
ADP/ATP translocase 1
ANT 1
ADP,ATP carrier protein 1
solute carrier family 25 member 4
heart/skeletal muscle ATP/ADP translocator
ADP,ATP carrier protein, heart/skeletal muscle
adenine nucleotide translocator 1 (skeletal muscle)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

4

4q35

Summary This gene is a member of the mitochondrial carrier subfamily of solute carrier protein genes. The product of this gene functions as a gated pore that translocates ADP from the cytoplasm into the mitochondrial matrix and ATP from the mitochondrial matrix into the cytoplasm. The protein forms a homodimer embedded in the inner mitochondria membrane. Mutations in this gene have been shown to result in autosomal dominant progressive external opthalmoplegia and familial hypertrophic cardiomyopathy. [provided by RefSeq, Jun 2013]. lac of sum
Disorder MIM:

103220

Disorder Html: Progressive external ophthalmoplegia with mitochondrial DNA

mRNA and Protein(s)

mRNA Protein Name
NM_001151 NP_001142 ADP/ATP translocase 1



Homo sapiens (human) SLC25A4 NP_001142.2
Pan troglodytes (chimpanzee) SLC25A4 XP_517556.3
Macaca mulatta (Rhesus monkey) SLC25A4 XP_001084544.1
Canis lupus familiaris (dog) SLC25A4 XP_532844.2
Bos taurus (cattle) SLC25A4 NP_777083.1
Mus musculus (house mouse) Slc25a4 NP_031476.3
Rattus norvegicus (Norway rat) Slc25a4 NP_445967.1
Gallus gallus (chicken) SLC25A4 NP_001006443.1
Danio rerio (zebrafish) slc25a4 NP_999867.1
Drosophila melanogaster (fruit fly) sesB NP_727448.1
Xenopus (Silurana) tropicalis (western clawed frog) slc25a4 NP_988909.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SLC25A4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC25A4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu22413
NM_001151 Homo sapiens solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 4 (SLC25A4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
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$199.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22413
Clone ID Related Accession (Same CDS sequence) NM_001151
Accession Version NM_001151.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 897bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-JUL-2015
Organism Homo sapiens (human)
Product ADP/ATP translocase 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI544547.1, BC008664.1, AC093824.3, AC112237.1 and AC093592.3. This sequence is a reference standard in the RefSeqGene project. On Sep 15, 2009 this sequence version replaced gi:55749576. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC008664.1, J02966.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGGTGATC ACGCTTGGAG CTTCCTAAAG GACTTCCTGG CCGGGGGCGT CGCCGCTGCC 
GTCTCCAAGA CCGCGGTCGC CCCCATCGAG AGGGTCAAAC TGCTGCTGCA GGTCCAGCAT
GCCAGCAAAC AGATCAGTGC TGAGAAGCAG TACAAAGGGA TCATTGATTG TGTGGTGAGA
ATCCCTAAGG AGCAGGGCTT CCTCTCCTTC TGGAGGGGTA ACCTGGCCAA CGTGATCCGT
TACTTCCCCA CCCAAGCTCT CAACTTCGCC TTCAAGGACA AGTACAAGCA GCTCTTCTTA
GGGGGTGTGG ATCGGCATAA GCAGTTCTGG CGCTACTTTG CTGGTAACCT GGCGTCCGGT
GGGGCCGCTG GGGCCACCTC CCTTTGCTTT GTCTACCCGC TGGACTTTGC TAGGACCAGG
TTGGCTGCTG ATGTGGGCAA GGGCGCCGCC CAGCGTGAGT TCCATGGTCT GGGCGACTGT
ATCATCAAGA TCTTCAAGTC TGATGGCCTG AGGGGGCTCT ACCAGGGTTT CAACGTCTCT
GTCCAAGGCA TCATTATCTA TAGAGCTGCC TACTTCGGAG TCTATGATAC TGCCAAGGGG
ATGCTGCCTG ACCCCAAGAA CGTGCACATT TTTGTGAGCT GGATGATTGC CCAGAGTGTG
ACGGCAGTCG CAGGGCTGGT GTCCTACCCC TTTGACACTG TTCGTCGTAG AATGATGATG
CAGTCCGGCC GGAAAGGGGC CGATATTATG TACACGGGGA CAGTTGACTG CTGGAGGAAG
ATTGCAAAAG ACGAAGGAGC CAAGGCCTTC TTCAAAGGTG CCTGGTCCAA TGTGCTGAGA
GGCATGGGCG GTGCTTTTGT ATTGGTGTTG TATGATGAGA TCAAAAAATA TGTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001142.2
CDS111..1007
Misc Feature(1)90..92(+)
Misc Feature(2)114..116(+)
Misc Feature(3)123..227(+)
Misc Feature(4)126..404(+)
Misc Feature(5)129..1004(+)
Misc Feature(6)129..413(+)
Misc Feature(7)333..410(+)
Misc Feature(8)435..539(+)
Misc Feature(9)438..728(+)
Misc Feature(10)441..713(+)
Misc Feature(11)549..551(+)
Misc Feature(12)588..590(+)
Misc Feature(13)636..716(+)
Misc Feature(14)729..833(+)
Misc Feature(15)744..1001(+)
Misc Feature(16)774..1001(+)
Misc Feature(17)813..830(+)
Misc Feature(18)843..845(+)
Misc Feature(19)924..926(+)
Misc Feature(20)927..1004(+)
Exon (1)1..221
Gene:SLC25A4
Gene Synonym:
Exon (2)222..708
Gene:SLC25A4
Gene Synonym:
Exon (3)709..849
Gene:SLC25A4
Gene Synonym:
Exon (4)850..4420
Gene:SLC25A4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001151
Organism Homo sapiens (human)
Definition Homo sapiens solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 4 (SLC25A4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001151

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGGTGATC ACGCTTGGAG CTTCCTAAAG GACTTCCTGG CCGGGGGCGT CGCCGCTGCC 
GTCTCCAAGA CCGCGGTCGC CCCCATCGAG AGGGTCAAAC TGCTGCTGCA GGTCCAGCAT
GCCAGCAAAC AGATCAGTGC TGAGAAGCAG TACAAAGGGA TCATTGATTG TGTGGTGAGA
ATCCCTAAGG AGCAGGGCTT CCTCTCCTTC TGGAGGGGTA ACCTGGCCAA CGTGATCCGT
TACTTCCCCA CCCAAGCTCT CAACTTCGCC TTCAAGGACA AGTACAAGCA GCTCTTCTTA
GGGGGTGTGG ATCGGCATAA GCAGTTCTGG CGCTACTTTG CTGGTAACCT GGCGTCCGGT
GGGGCCGCTG GGGCCACCTC CCTTTGCTTT GTCTACCCGC TGGACTTTGC TAGGACCAGG
TTGGCTGCTG ATGTGGGCAA GGGCGCCGCC CAGCGTGAGT TCCATGGTCT GGGCGACTGT
ATCATCAAGA TCTTCAAGTC TGATGGCCTG AGGGGGCTCT ACCAGGGTTT CAACGTCTCT
GTCCAAGGCA TCATTATCTA TAGAGCTGCC TACTTCGGAG TCTATGATAC TGCCAAGGGG
ATGCTGCCTG ACCCCAAGAA CGTGCACATT TTTGTGAGCT GGATGATTGC CCAGAGTGTG
ACGGCAGTCG CAGGGCTGGT GTCCTACCCC TTTGACACTG TTCGTCGTAG AATGATGATG
CAGTCCGGCC GGAAAGGGGC CGATATTATG TACACGGGGA CAGTTGACTG CTGGAGGAAG
ATTGCAAAAG ACGAAGGAGC CAAGGCCTTC TTCAAAGGTG CCTGGTCCAA TGTGCTGAGA
GGCATGGGCG GTGCTTTTGT ATTGGTGTTG TATGATGAGA TCAAAAAATA TGTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Mitochondrial DNA content contributes to healthy aging in Chinese: a study from nonagenarians and centenarians
Neurobiol. Aging 35 (7), 1779 (2014)
He YH, Lu X, Wu H, Cai WW, Yang LQ, Xu LY, Sun HP and Kong QP.


book

Adenine nucleotide translocase is acetylated in vivo in human muscle: Modeling predicts a decreased ADP affinity and altered control of oxidative phosphorylation
Biochemistry 53 (23), 3817-3829 (2014)
Mielke C, Lefort N, McLean CG, Cordova JM, Langlais PR, Bordner AJ, Te JA, Ozkan SB, Willis WT and Mandarino LJ.


book

Ribosomal protein s15 phosphorylation mediates LRRK2 neurodegeneration in Parkinson's disease
Cell 157 (2), 472-485 (2014)
Martin I, Kim JW, Lee BD, Kang HC, Xu JC, Jia H, Stankowski J, Kim MS, Zhong J, Kumar M, Andrabi SA, Xiong Y, Dickson DW, Wszolek ZK, Pandey A, Dawson TM and Dawson VL.


book

Adenine nucleotide translocase 1 expression affects enterovirus infection in human and murine hearts
Int. J. Cardiol. 172 (3), E449-E452 (2014)
Kuhl U, Ebermann L, Lassner D, Klingel K, Klumpe I, Winter J, Zeichhardt H, Schultheiss HP and Dorner A.


book

Severity of cardiomyopathy associated with adenine nucleotide translocator-1 deficiency correlates with mtDNA haplogroup
Proc. Natl. Acad. Sci. U.S.A. 110 (9), 3453-3458 (2013)
Strauss KA, DuBiner L, Simon M, Zaragoza M, Sengupta PP, Li P, Narula N, Dreike S, Platt J, Procaccio V, Ortiz-Gonzalez XR, Puffenberger EG, Kelley RI, Morton DH, Narula J and Wallace DC.


book

Assignment of the human muscle adenine nucleotide translocator gene (ANT1) to 4q35 by fluorescence in situ hybridization
Cytogenet. Cell Genet. 60 (1), 29-30 (1992)
Fan YS, Yang HM and Lin CC.


book

A human muscle adenine nucleotide translocator gene has four exons, is located on chromosome 4, and is differentially expressed
J. Biol. Chem. 264 (24), 13998-14004 (1989)
Li K, Warner CK, Hodge JA, Minoshima S, Kudoh J, Fukuyama R, Maekawa M, Shimizu Y, Shimizu N and Wallace DC.


book

DNA sequences of two expressed nuclear genes for human mitochondrial ADP/ATP translocase
J. Mol. Biol. 206 (2), 261-280 (1989)
Cozens AL, Runswick MJ and Walker JE.


book

Two distinct genes for ADP/ATP translocase are expressed at the mRNA level in adult human liver
Proc. Natl. Acad. Sci. U.S.A. 85 (2), 377-381 (1988)
Houldsworth J and Attardi G.


book

cDNA sequence of a human skeletal muscle ADP/ATP translocator: lack of a leader peptide, divergence from a fibroblast translocator cDNA, and coevolution with mitochondrial DNA genes
Proc. Natl. Acad. Sci. U.S.A. 84 (21), 7580-7584 (1987)
Neckelmann N, Li K, Wade RP, Shuster R and Wallace DC.


 
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