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GUSB glucuronidase, beta [Homo sapiens (human)]

Gene Symbol GUSB
Entrez Gene ID 2990
Full Name glucuronidase, beta
Synonyms BG, MPS7
General protein information
Preferred Names
beta-glucuronidase
Names
beta-glucuronidase
beta-G1
beta-D-glucuronidase
NP_000172.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7q21.11

Summary This gene encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. The enzyme forms a homotetramer that is localized to the lysosome. Mutations in this gene result in mucopolysaccharidosis type VII. Alternative splicing results in multiple transcript variants. There are many pseudogenes of this locus in the human genome.[provided by RefSeq, May 2014]. lac of sum
Disorder MIM:

611499

Disorder Html: Mucopolysaccharidosis VII, 253220 (3)





The following GUSB gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GUSB gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu41505 XM_005250297 PREDICTED: Homo sapiens glucuronidase, beta (GUSB), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu58165 XM_011516113 PREDICTED: Homo sapiens glucuronidase, beta (GUSB), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu58166 XM_011516114 PREDICTED: Homo sapiens glucuronidase, beta (GUSB), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu27736 NM_001284290 Homo sapiens glucuronidase, beta (GUSB), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu27738 NM_001293104 Homo sapiens glucuronidase, beta (GUSB), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu27739 NM_001293105 Homo sapiens glucuronidase, beta (GUSB), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu27733 NM_000181 Homo sapiens glucuronidase, beta (GUSB), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.3
$99

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu41505
Accession Version XM_005250297.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1803bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product beta-glucuronidase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578814172. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGCCCGGG GGTCGGCGGT TGCCTGGGCG GCGCTCGGGC CGTTGTTGTG GGGCTGCGCG 
CTGGGGCTGC AGGGCGGGAT GCTGTACCCC CAGGAGAGCC CGTCGCGGGA GTGCAAGGAG
CTGGACGGCC TCTGGAGCTT CCGCGCCGAC TTCTCTGACA ACCGACGCCG GGGCTTCGAG
GAGCAGTGGT ACCGGCGGCC GCTGTGGGAG TCAGGCCCCA CCGTGGACAT GCCAGTTCCC
TCCAGCTTCA ATGACATCAG CCAGGACTGG CGTCTGCGGC ATTTTGTCGG CTGGGTGTGG
TACGAACGGG AGGTGATCCT GCCGGAGCGA TGGACCCAGG ACCTGCGCAC AAGAGTGGTG
CTGAGGATTG GCAGTGCCCA TTCCTATGCC ATCGTGTGGG TGAATGGGGT CGACACGCTA
GAGCATGAGG GGGGCTACCT CCCCTTCGAG GCCGACATCA GCAACCTGGT CCAGGTGGGG
CCCCTGCCCT CCCGGCTCCG AATCACTATC GCCATCAACA ACACACTCAC CCCCACCACC
CTGCCACCAG GGACCATCCA ATACCTGACT GACACCTCCA AGTATCCCAA GGGTTACTTT
GTCCAGAACA CATATTTTGA CTTTTTCAAC TACGCTGGAC TGCAGCGGTC TGTACTTCTG
TACACGACAC CCACCACCTA CATCGATGAC ATCACCGTCA CCACCAGCGT GGAGCAAGAC
AGTGGGCTGG TGAATTACCA GATCTCTGTC AAGGGCAGTA ACCTGTTCAA GTTGGAAGTG
CGTCTTTTGG ATGCAGAAAA CAAAGTCGTG GCGAATGGGA CTGGGACCCA GGGCCAACTT
AAGGTGCCAG GTGTCAGCCT CTGGTGGCCG TACCTGATGC ACGAACGCCC TGCCTATCTG
TATTCATTGG AGATCCGAGG GAAGGGCTTC GACTGGCCGC TGCTGGTGAA GGACTTCAAC
CTGCTTCGCT GGCTTGGTGC CAACGCTTTC CGTACCAGCC ACTACCCCTA TGCAGAGGAA
GTGATGCAGA TGTGTGACCG CTATGGGATT GTGGTCATCG ATGAGTGTCC CGGCGTGGGC
CTGGCGCTGC CGCAGTTCTT CAACAACGTT TCTCTGCATC ACCACATGCA GGTGATGGAA
GAAGTGGTGC GTAGGGACAA GAACCACCCC GCGGTCGTGA TGTGGTCTGT GGCCAACGAG
CCTGCGTCCC ACCTAGAATC TGCTGGCTAC TACTTGAAGA TGGTGATCGC TCACACCAAA
TCCTTGGACC CCTCCCGGCC TGTGACCTTT GTGAGCAACT CTAACTATGC AGCAGACAAG
GGGGCTCCGT ATGTGGATGT GATCTGTTTG AACAGCTACT ACTCTTGGTA TCACGACTAC
GGGCACCTGG AGTTGATTCA GCTGCAGCTG GCCACCCAGT TTGAGAACTG GTATAAGAAG
TATCAGAAGC CCATTATTCA GAGCGAGTAT GGAGCAGAAA CGATTGCAGG GTTTCACCAG
GATCCACCTC TGATGTTCAC TGAAGAGTAC CAGAAAAGTC TGCTAGAGCA GTACCATCTG
GGTCTGGATC AAAAACGCAG AAAATACGTG GTTGGAGAGC TCATTTGGAA TTTTGCCGAT
TTCATGACTG AACAGTCACC GACGAGAGTG CTGGGGAATA AAAAGGGGAT CTTCACTCGG
CAGAGACAAC CAAAAAGTGC AGCGTTCCTT TTGCGAGAGA GATACTGGAA GATTGCCAAT
GAAACCAGGT ATCCCCACTC AGTAGCCAAG TCACAATGTT TGGAAAACAG CCTGTTTACT
TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005250354.1
CDS127..1929
Misc Feature(1)205..1866(+)
Misc Feature(2)229..798(+)
Misc Feature(3)802..1044(+)
Misc Feature(4)1039..1866(+)
Translation
Position Chain Variation Link
15 15 c, g dbSNP:549392182
32 32 a, g dbSNP:748697743
48 48 g, t dbSNP:528932289
55 55 g, t dbSNP:2279904
75 75 c, g dbSNP:777048370
77 77 c, t dbSNP:201504866
85 85 g, t dbSNP:199591754
94 94 c, g dbSNP:760455943
107 107 c, g, t dbSNP:577269089
108 108 c, g dbSNP:771999951
111 111 c, t dbSNP:745843396
112 112 c, g dbSNP:781149654
115 115 a, g dbSNP:2279903
117 117 a, t dbSNP:540793200
120 120 a, g dbSNP:777339764
125 125 g, t dbSNP:755795388
126 126 c, t dbSNP:747348554
131 131 c, t dbSNP:780330659
132 132 c, g dbSNP:758903707
133 133 c, t dbSNP:750955623
134 134 g, t dbSNP:765390811
135 135 g, t dbSNP:757357472
136 136 c, g dbSNP:572131903
138 138 a, g dbSNP:753976305
139 139 c, t dbSNP:190496263
140 140 c, t dbSNP:535382125
142 142 a, g dbSNP:775335168
148 148 g, t dbSNP:376505707
180 180 c, t dbSNP:759514074
183 183 c, g, t dbSNP:573989102
210 210 a, g dbSNP:748756976
212 212 a, g dbSNP:772729596
214 214 a, c, t dbSNP:747792546
216 216 c, t dbSNP:745503914
226 226 c, t dbSNP:780459919
231 231 c, g dbSNP:758743865
237 237 g, t dbSNP:746254536
238 238 c, t dbSNP:779499448
244 244 a, g dbSNP:757874872
250 250 g, t dbSNP:753922918
264 264 c, g dbSNP:764321712
273 273 c, t dbSNP:375940373
276 276 c, t dbSNP:752931066
283 283 a, g dbSNP:371314015
287 287 a, g dbSNP:557347158
288 288 c, t dbSNP:74975849
292 292 c, t dbSNP:147262894
295 295 c, g, t dbSNP:772676579
296 296 g, t dbSNP:769252159
297 297 a, g dbSNP:567066124
311 311 a, g dbSNP:571736757
322 322 c, t dbSNP:776085845
323 323 g, t dbSNP:768362767
327 327 g, t dbSNP:551892631
328 328 c, t dbSNP:746199104
342 342 c, t dbSNP:77774169
343 343 a, c dbSNP:748127836
348 348 c, g, t dbSNP:140016611
356 356 g, t dbSNP:751845855
358 358 c, g dbSNP:779807872
359 359 c, t dbSNP:758127615
375 375 c, t dbSNP:750221073
377 377 a, g dbSNP:765042077
385 385 a, g dbSNP:757243831
386 386 g, t dbSNP:753270240
387 387 c, t dbSNP:112435030
392 392 a, g dbSNP:398123236
397 397 c, t dbSNP:763704693
398 398 a, g dbSNP:147454954
403 403 c, t dbSNP:769047951
404 404 a, g dbSNP:767292825
412 412 g, t dbSNP:763344202
414 414 c, t dbSNP:773646634
415 415 a, g dbSNP:770176365
416 416 a, g dbSNP:748729882
421 421 a, g dbSNP:398123237
422 422 g, t dbSNP:776569076
429 429 c, t dbSNP:768778881
433 433 c, t dbSNP:786205673
439 439 a, g dbSNP:747101015
450 450 a, g dbSNP:546131174
457 457 g, t dbSNP:200935970
459 459 c, g dbSNP:745604255
466 466 g, t dbSNP:778819412
472 472 c, g, t dbSNP:753883162
473 473 a, g, t dbSNP:538431094
477 477 a, g dbSNP:532399422
478 478 a, g dbSNP:767239546
487 487 c, g dbSNP:759309329
491 491 a, g dbSNP:773416756
506 506 a, g dbSNP:765699404
509 509 c, t dbSNP:762212333
517 517 a, g dbSNP:777134888
520 520 a, g dbSNP:769151272
524 524 c, g dbSNP:786205671
533 533 c, g dbSNP:749073290
537 537 c, t dbSNP:141441283
538 538 a, c, g dbSNP:748161388
542 542 c, t dbSNP:780835314
546 546 a, c dbSNP:754452177
555 555 a, c, g, t dbSNP:375740757
568 568 c, t dbSNP:121918177
573 573 c, t dbSNP:148364905
576 576 c, g dbSNP:764486500
579 579 c, g, t dbSNP:144397476
580 580 a, g dbSNP:149606212
618 618 c, t dbSNP:759593913
619 619 c, g, t dbSNP:771137253
624 624 c, g, t dbSNP:773134072
625 625 c, t dbSNP:769769379
628 628 a, g dbSNP:747969208
634 634 a, g dbSNP:781205550
636 636 c, t dbSNP:752674900
637 637 a, g dbSNP:746475728
640 640 a, g dbSNP:369537067
645 645 c, t dbSNP:779475930
652 652 c, t dbSNP:121918181
653 653 -, tcac dbSNP:776287966
656 656 c, t dbSNP:587779400
658 658 a, c dbSNP:11559281
659 659 c, t dbSNP:778102597
666 666 c, t dbSNP:138435796
671 671 c, t dbSNP:756417262
674 674 c, t dbSNP:753065037
678 678 a, g dbSNP:768029322
679 679 a, c dbSNP:755465262
686 686 a, g dbSNP:751638113
687 687 a, g dbSNP:74430256
696 696 c, t dbSNP:763253448
709 709 c, t dbSNP:372083359
737 737 c, t dbSNP:748981851
738 738 a, g dbSNP:781604428
739 739 c, t dbSNP:367989163
750 750 c, t dbSNP:747403896
754 754 a, t dbSNP:200457516
759 759 c, t dbSNP:140278510
763 763 a, g dbSNP:750505590
767 767 g, t dbSNP:779171218
770 770 a, t dbSNP:757545827
772 772 c, t dbSNP:121918174
783 783 g, t dbSNP:764393614
784 784 c, g dbSNP:760324067
789 789 c, g dbSNP:752571900
790 790 a, g dbSNP:767394322
791 791 c, t dbSNP:759521075
792 792 a, g dbSNP:773822519
795 795 a, t dbSNP:201178083
800 800 c, g dbSNP:773636939
804 804 c, t dbSNP:537957979
808 808 a, g dbSNP:772672861
810 810 a, c, t dbSNP:375590538
811 811 a, g dbSNP:780513114
813 813 g, t dbSNP:772673266
821 821 c, g dbSNP:138618819
822 822 c, t dbSNP:779124500
823 823 a, g dbSNP:202210104
833 833 c, g dbSNP:753959306
834 834 c, t dbSNP:777822251
835 835 a, g, t dbSNP:373800055
848 848 a, g dbSNP:200298579
864 864 c, g dbSNP:764866250
875 875 g, t dbSNP:761355885
886 886 a, c dbSNP:150086524
890 890 a, t dbSNP:140709901
892 892 c, t dbSNP:200941434
894 894 c, g dbSNP:774882132
905 905 c, t dbSNP:771320756
907 907 c, t dbSNP:768075787
908 908 a, g dbSNP:373590814
910 910 c, t dbSNP:769976411
922 922 a, g dbSNP:748229629
931 931 a, c, g dbSNP:755211573
933 933 c, t dbSNP:571704452
938 938 c, t dbSNP:369465888
939 939 a, g dbSNP:150999162
954 954 c, t dbSNP:758263587
963 963 a, g dbSNP:750430817
968 968 a, g dbSNP:765097533
974 974 c, t dbSNP:779460964
976 976 c, g dbSNP:756721679
987 987 c, t dbSNP:753430922
992 992 a, g dbSNP:398123238
996 996 a, g dbSNP:377665214
997 997 a, t dbSNP:760452541
1000 1000 c, g dbSNP:751945330
1008 1008 c, t dbSNP:766837276
1011 1011 a, g dbSNP:763358124
1012 1012 c, t dbSNP:773699561
1013 1013 a, g dbSNP:528899475
1030 1030 c, t dbSNP:761828939
1031 1031 a, c dbSNP:776736558
1033 1033 c, t dbSNP:569297611
1042 1042 c, t dbSNP:121918185
1056 1056 c, t dbSNP:139976112
1057 1057 a, g dbSNP:398123234
1064 1064 c, t dbSNP:771629102
1065 1065 a, g dbSNP:759063684
1069 1069 c, g dbSNP:535768789
1072 1072 a, g dbSNP:770650835
1083 1083 c, g dbSNP:374490058
1089 1089 a, g dbSNP:778654745
1093 1093 c, g, t dbSNP:747572640
1094 1094 a, g dbSNP:780716481
1099 1099 c, t dbSNP:11559283
1107 1107 c, t dbSNP:374755881
1110 1110 a, c, g, t dbSNP:371271170
1111 1111 a, g dbSNP:377615121
1115 1115 c, t dbSNP:74410123
1117 1117 c, t dbSNP:121918173
1118 1118 c, g dbSNP:764018631
1119 1119 c, t dbSNP:756033004
1120 1120 a, c dbSNP:200451572
1130 1130 a, g dbSNP:767451327
1136 1136 a, g dbSNP:759010501
1140 1140 a, g dbSNP:757058191
1142 1142 a, g dbSNP:398123235
1155 1155 a, g dbSNP:369190724
1158 1158 g, t dbSNP:149761698
1165 1165 a, c, t dbSNP:749520560
1166 1166 a, g dbSNP:776843811
1168 1168 c, t dbSNP:376589676
1169 1169 a, g dbSNP:556424939
1185 1185 c, t dbSNP:375214425
1186 1186 a, g dbSNP:200149500
1188 1188 c, t dbSNP:745919844
1192 1192 -, c dbSNP:761247245
1195 1195 c, t dbSNP:779091113
1197 1197 c, t dbSNP:757418833
1198 1198 a, g dbSNP:749582075
1200 1200 c, t dbSNP:777871501
1201 1201 a, g dbSNP:755842317
1209 1209 a, g dbSNP:747608667
1211 1211 c, t dbSNP:752523400
1212 1212 a, g dbSNP:767396235
1213 1213 c, t dbSNP:150686327
1215 1215 c, g dbSNP:367551992
1217 1217 c, t dbSNP:751025746
1226 1226 g, t dbSNP:193920810
1229 1229 a, g dbSNP:771376085
1234 1234 a, g dbSNP:763043756
1240 1240 c, t dbSNP:773334034
1244 1244 a, g dbSNP:769976774
1248 1248 c, t dbSNP:748423878
1258 1258 a, g dbSNP:376131143
1261 1261 a, g dbSNP:768428238
1262 1262 c, t dbSNP:766849255
1264 1264 a, g dbSNP:143040697
1270 1270 a, g dbSNP:369767887
1273 1273 -, gtg dbSNP:768543128
1276 1276 c, t dbSNP:758314777
1277 1277 a, g dbSNP:749917538
1286 1286 a, g dbSNP:778631803
1292 1292 -, c dbSNP:35957142
1296 1296 c, t dbSNP:368144710
1297 1297 a, g dbSNP:753483823
1298 1298 c, t dbSNP:763845326
1302 1302 c, t dbSNP:139776224
1308 1308 a, g dbSNP:751894676
1311 1311 a, g dbSNP:121918180
1323 1323 a, c, t dbSNP:376567545
1332 1332 a, g dbSNP:118066970
1334 1334 c, g dbSNP:769803627
1342 1342 a, g dbSNP:761881997
1356 1356 c, t dbSNP:776791543
1374 1374 c, t dbSNP:764344489
1375 1375 a, g dbSNP:752854143
1378 1378 c, t dbSNP:775341321
1388 1388 c, t dbSNP:771707097
1389 1389 c, t dbSNP:138554083
1392 1392 a, g dbSNP:745662610
1402 1402 c, t dbSNP:774393243
1403 1403 a, g dbSNP:771017036
1404 1404 a, g dbSNP:748788618
1416 1416 c, t dbSNP:777191171
1427 1427 c, t dbSNP:755791810
1434 1434 c, t dbSNP:200831987
1443 1443 c, t dbSNP:143084006
1445 1445 -, a dbSNP:771250055
1453 1453 c, g dbSNP:143495981
1454 1454 c, t dbSNP:747719992
1455 1455 a, g dbSNP:548880426
1457 1457 a, g dbSNP:121918178
1470 1470 c, g dbSNP:760449944
1484 1484 a, g dbSNP:768343264
1488 1488 c, t dbSNP:746198220
1494 1494 a, g dbSNP:121918179
1499 1499 -, a dbSNP:778161730
1500 1500 a, c, t dbSNP:201928248
1501 1501 a, g dbSNP:778349139
1505 1505 a, g dbSNP:199694121
1506 1506 c, t dbSNP:752845837
1526 1526 a, c dbSNP:767826344
1534 1534 c, g dbSNP:759880257
1555 1555 c, t dbSNP:751397640
1558 1558 c, t dbSNP:767730315
1559 1559 a, g dbSNP:786205674
1560 1560 c, t dbSNP:766339888
1564 1564 -, aag dbSNP:758492902
1578 1578 c, t dbSNP:762859080
1590 1590 c, t dbSNP:377519272
1593 1593 a, g dbSNP:368053364
1596 1596 -, t dbSNP:756256226
1596 1596 c, t dbSNP:769788461
1608 1608 a, g dbSNP:761204529
1617 1617 a, g dbSNP:776242697
1626 1626 c, g dbSNP:768292111
1627 1627 c, g dbSNP:760181741
1636 1636 c, t dbSNP:775084641
1653 1653 a, g dbSNP:771735289
1660 1660 a, c dbSNP:749594772
1667 1667 g, t dbSNP:149448152
1668 1668 c, g dbSNP:770200580
1672 1672 a, g dbSNP:748750514
1698 1698 a, c dbSNP:781768877
1700 1700 a, g dbSNP:755045141
1702 1702 c, t dbSNP:747104326
1703 1703 g, t dbSNP:121918183
1711 1711 c, t dbSNP:780397637
1713 1713 c, t dbSNP:1061361
1714 1714 a, g dbSNP:150304382
1715 1715 c, t dbSNP:765136502
1719 1719 c, t dbSNP:373819742
1720 1720 a, g dbSNP:757015172
1725 1725 a, g dbSNP:141430018
1729 1729 a, t dbSNP:764050758
1743 1743 a, c, t dbSNP:200228580
1750 1750 a, g dbSNP:113059520
1752 1752 a, c, g dbSNP:564360146
1761 1761 a, g dbSNP:759301811
1764 1764 a, g dbSNP:767115386
1766 1766 c, t dbSNP:758984878
1767 1767 a, g dbSNP:751161248
1769 1769 c, t dbSNP:765983374
1770 1770 a, g dbSNP:552724524
1775 1775 g, t dbSNP:762241590
1795 1795 a, c dbSNP:777173084
1804 1804 c, t dbSNP:121918176
1818 1818 -, a dbSNP:35302712
1818 1818 a, t dbSNP:761319717
1823 1823 g, t dbSNP:775873162
1826 1826 c, g dbSNP:772122474
1829 1829 c, t dbSNP:121918172
1830 1830 a, g dbSNP:779147312
1840 1840 c, t dbSNP:751840849
1847 1847 a, g dbSNP:372550726
1849 1849 c, t dbSNP:777613366
1854 1854 g, t dbSNP:121918184
1860 1860 g, t dbSNP:756035101
1864 1864 a, c dbSNP:752576315
1865 1865 a, g dbSNP:780999350
1871 1871 c, t dbSNP:754474825
1874 1874 c, g dbSNP:751075889
1875 1875 a, g dbSNP:766019399
1878 1878 -, t dbSNP:762840002
1879 1879 c, t dbSNP:145910162
1880 1880 a, c dbSNP:762757920
1891 1891 -, tag dbSNP:770237165
1898 1898 c, t dbSNP:754238750
1902 1902 a, g dbSNP:764493374
1914 1914 -, c dbSNP:372007777
1915 1915 a, c, g dbSNP:776024156
1918 1918 c, t dbSNP:772478693
1919 1919 c, t dbSNP:9530
1919 1919 c, t dbSNP:544927524
1920 1920 a, g dbSNP:774336516
1925 1925 c, g dbSNP:557221338
1927 1927 g, t dbSNP:771138602
1933 1933 a, t dbSNP:201204553
1941 1941 a, c, g dbSNP:769514976
1942 1942 a, c dbSNP:747928978
1947 1947 -, t dbSNP:769662185
1949 1949 c, t dbSNP:150311243
1950 1950 a, g dbSNP:754908402
1952 1952 a, g dbSNP:751073317
1954 1954 c, t dbSNP:779485917
1963 1963 c, g dbSNP:62470933
1966 1966 a, g dbSNP:757993425
1970 1970 c, g, t dbSNP:765012426
1975 1975 c, t dbSNP:756497692
1979 1979 a, t dbSNP:753051726
1984 1984 c, t dbSNP:372605666
1998 1998 c, g dbSNP:753596986
2016 2016 a, g dbSNP:567259021
2023 2023 a, g dbSNP:536903750
2077 2077 a, g dbSNP:568655640
2090 2090 a, g dbSNP:140236850
2101 2101 -, a dbSNP:762261099
2101 2101 a, g dbSNP:77293332
2123 2123 a, g dbSNP:138519677
2137 2137 g, t dbSNP:190201470

Target ORF information:

RefSeq Version XM_005250297
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens glucuronidase, beta (GUSB), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005250297

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGCCCGGG GGTCGGCGGT TGCCTGGGCG GCGCTCGGGC CGTTGTTGTG GGGCTGCGCG 
CTGGGGCTGC AGGGCGGGAT GCTGTACCCC CAGGAGAGCC CGTCGCGGGA GTGCAAGGAG
CTGGACGGCC TCTGGAGCTT CCGCGCCGAC TTCTCTGACA ACCGACGCCG GGGCTTCGAG
GAGCAGTGGT ACCGGCGGCC GCTGTGGGAG TCAGGCCCCA CCGTGGACAT GCCAGTTCCC
TCCAGCTTCA ATGACATCAG CCAGGACTGG CGTCTGCGGC ATTTTGTCGG CTGGGTGTGG
TACGAACGGG AGGTGATCCT GCCGGAGCGA TGGACCCAGG ACCTGCGCAC AAGAGTGGTG
CTGAGGATTG GCAGTGCCCA TTCCTATGCC ATCGTGTGGG TGAATGGGGT CGACACGCTA
GAGCATGAGG GGGGCTACCT CCCCTTCGAG GCCGACATCA GCAACCTGGT CCAGGTGGGG
CCCCTGCCCT CCCGGCTCCG AATCACTATC GCCATCAACA ACACACTCAC CCCCACCACC
CTGCCACCAG GGACCATCCA ATACCTGACT GACACCTCCA AGTATCCCAA GGGTTACTTT
GTCCAGAACA CATATTTTGA CTTTTTCAAC TACGCTGGAC TGCAGCGGTC TGTACTTCTG
TACACGACAC CCACCACCTA CATCGATGAC ATCACCGTCA CCACCAGCGT GGAGCAAGAC
AGTGGGCTGG TGAATTACCA GATCTCTGTC AAGGGCAGTA ACCTGTTCAA GTTGGAAGTG
CGTCTTTTGG ATGCAGAAAA CAAAGTCGTG GCGAATGGGA CTGGGACCCA GGGCCAACTT
AAGGTGCCAG GTGTCAGCCT CTGGTGGCCG TACCTGATGC ACGAACGCCC TGCCTATCTG
TATTCATTGG AGATCCGAGG GAAGGGCTTC GACTGGCCGC TGCTGGTGAA GGACTTCAAC
CTGCTTCGCT GGCTTGGTGC CAACGCTTTC CGTACCAGCC ACTACCCCTA TGCAGAGGAA
GTGATGCAGA TGTGTGACCG CTATGGGATT GTGGTCATCG ATGAGTGTCC CGGCGTGGGC
CTGGCGCTGC CGCAGTTCTT CAACAACGTT TCTCTGCATC ACCACATGCA GGTGATGGAA
GAAGTGGTGC GTAGGGACAA GAACCACCCC GCGGTCGTGA TGTGGTCTGT GGCCAACGAG
CCTGCGTCCC ACCTAGAATC TGCTGGCTAC TACTTGAAGA TGGTGATCGC TCACACCAAA
TCCTTGGACC CCTCCCGGCC TGTGACCTTT GTGAGCAACT CTAACTATGC AGCAGACAAG
GGGGCTCCGT ATGTGGATGT GATCTGTTTG AACAGCTACT ACTCTTGGTA TCACGACTAC
GGGCACCTGG AGTTGATTCA GCTGCAGCTG GCCACCCAGT TTGAGAACTG GTATAAGAAG
TATCAGAAGC CCATTATTCA GAGCGAGTAT GGAGCAGAAA CGATTGCAGG GTTTCACCAG
GATCCACCTC TGATGTTCAC TGAAGAGTAC CAGAAAAGTC TGCTAGAGCA GTACCATCTG
GGTCTGGATC AAAAACGCAG AAAATACGTG GTTGGAGAGC TCATTTGGAA TTTTGCCGAT
TTCATGACTG AACAGTCACC GACGAGAGTG CTGGGGAATA AAAAGGGGAT CTTCACTCGG
CAGAGACAAC CAAAAAGTGC AGCGTTCCTT TTGCGAGAGA GATACTGGAA GATTGCCAAT
GAAACCAGGT ATCCCCACTC AGTAGCCAAG TCACAATGTT TGGAAAACAG CCTGTTTACT
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58165
Accession Version XM_011516113.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1455bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product beta-glucuronidase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGACCCAG GACCTGCGCA CAAGAGTGGT GCTGAGGATT GGCAGTGCCC ATTCCTATGC 
CATCGTGGTA TCCCAAGGGT TACTTTGTCC AGAACACATA TTTTGACTTT TTCAACTACG
CTGGACTGCA GCGGTCTGTA CTTCTGTACA CGACACCCAC CACCTACATC GATGACATCA
CCGTCACCAC CAGCGTGGAG CAAGACAGTG GTGAGGGCTT CTGGGCTGGT GAATTACCAG
ATCTCTGTCA AGGGCAGTAA CCTGTTCAAG TTGGAAGTGC GTCTTTTGGA TGCAGAAAAC
AAAGTCGTGG CGAATGGGAC TGGGACCCAG GGCCAACTTA AGGTGCCAGG TGTCAGCCTC
TGGTGGCCGT ACCTGATGCA CGAACGCCCT GCCTATCTGT ATTCATTGGA GGTGCAGCTG
ACTGCACAGA CGTCACTGGG GCCTGTGTCT GACTTCTACA CACTCCCTGT GGGGATCCGC
ACTGTGGCTG TCACCAAGAG CCAGTTCCTC ATCAATGGGA AACCTTTCTA TTTCCACGGT
GTCAACAAGC ATGAGGATGC GGACATCCGA GGGAAGGGCT TCGACTGGCC GCTGCTGGTG
AAGGACTTCA ACCTGCTTCG CTGGCTTGGT GCCAACGCTT TCCGTACCAG CCACTACCCC
TATGCAGAGG AAGTGATGCA GATGTGTGAC CGCTATGGGA TTGTGGTCAT CGATGAGTGT
CCCGGCGTGG GCCTGGCGCT GCCGCAGTTC TTCAACAACG TTTCTCTGCA TCACCACATG
CAGGTGATGG AAGAAGTGGT GCGTAGGGAC AAGAACCACC CCGCGGTCGT GATGTGGTCT
GTGGCCAACG AGCCTGCGTC CCACCTAGAA TCTGCTGGCT ACTACTTGAA GATGGTGATC
GCTCACACCA AATCCTTGGA CCCCTCCCGG CCTGTGACCT TTGTGAGCAA CTCTAACTAT
GCAGCAGACA AGGGGGCTCC GTATGTGGAT GTGATCTGTT TGAACAGCTA CTACTCTTGG
TATCACGACT ACGGGCACCT GGAGTTGATT CAGCTGCAGC TGGCCACCCA GTTTGAGAAC
TGGTATAAGA AGTATCAGAA GCCCATTATT CAGAGCGAGT ATGGAGCAGA AACGATTGCA
GGGTTTCACC AGGATCCACC TCTGATGTTC ACTGAAGAGT ACCAGAAAAG TCTGCTAGAG
CAGTACCATC TGGGTCTGGA TCAAAAACGC AGAAAATACG TGGTTGGAGA GCTCATTTGG
AATTTTGCCG ATTTCATGAC TGAACAGTCA CCGACGAGAG TGCTGGGGAA TAAAAAGGGG
ATCTTCACTC GGCAGAGACA ACCAAAAAGT GCAGCGTTCC TTTTGCGAGA GAGATACTGG
AAGATTGCCA ATGAAACCAG GTATCCCCAC TCAGTAGCCA AGTCACAATG TTTGGAAAAC
AGCCTGTTTA CTTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011514415.1
CDS377..1831
Misc Feature(1)860..1768(+)
Translation
Position Chain Variation Link
6 6 g, t dbSNP:199591754
15 15 c, g dbSNP:760455943
28 28 c, g, t dbSNP:577269089
29 29 c, g dbSNP:771999951
32 32 c, t dbSNP:745843396
33 33 c, g dbSNP:781149654
36 36 a, g dbSNP:2279903
38 38 a, t dbSNP:540793200
41 41 a, g dbSNP:777339764
46 46 g, t dbSNP:755795388
47 47 c, t dbSNP:747348554
52 52 c, t dbSNP:780330659
53 53 c, g dbSNP:758903707
54 54 c, t dbSNP:750955623
55 55 g, t dbSNP:765390811
56 56 g, t dbSNP:757357472
57 57 c, g dbSNP:572131903
59 59 a, g dbSNP:753976305
60 60 c, t dbSNP:190496263
61 61 c, t dbSNP:535382125
63 63 a, g dbSNP:775335168
69 69 g, t dbSNP:376505707
101 101 c, t dbSNP:759514074
104 104 c, g, t dbSNP:573989102
131 131 a, g dbSNP:748756976
133 133 a, g dbSNP:772729596
135 135 a, c, t dbSNP:747792546
137 137 c, t dbSNP:745503914
147 147 c, t dbSNP:780459919
152 152 c, g dbSNP:758743865
158 158 g, t dbSNP:746254536
159 159 c, t dbSNP:779499448
165 165 a, g dbSNP:757874872
171 171 g, t dbSNP:753922918
185 185 c, g dbSNP:764321712
194 194 c, t dbSNP:375940373
197 197 c, t dbSNP:752931066
204 204 a, g dbSNP:371314015
208 208 a, g dbSNP:557347158
209 209 c, t dbSNP:74975849
213 213 c, t dbSNP:147262894
216 216 c, g, t dbSNP:772676579
217 217 g, t dbSNP:769252159
218 218 a, g dbSNP:567066124
232 232 a, g dbSNP:571736757
243 243 c, t dbSNP:776085845
244 244 g, t dbSNP:768362767
248 248 g, t dbSNP:551892631
249 249 c, t dbSNP:746199104
263 263 c, t dbSNP:77774169
264 264 a, c dbSNP:748127836
269 269 c, g, t dbSNP:140016611
277 277 g, t dbSNP:751845855
279 279 c, g dbSNP:779807872
280 280 c, t dbSNP:758127615
296 296 c, t dbSNP:750221073
298 298 a, g dbSNP:765042077
306 306 a, g dbSNP:757243831
307 307 g, t dbSNP:753270240
308 308 c, t dbSNP:112435030
313 313 a, g dbSNP:398123236
318 318 c, t dbSNP:763704693
319 319 a, g dbSNP:147454954
324 324 c, t dbSNP:769047951
325 325 a, g dbSNP:767292825
333 333 g, t dbSNP:763344202
335 335 c, t dbSNP:773646634
336 336 a, g dbSNP:770176365
337 337 a, g dbSNP:748729882
342 342 a, g dbSNP:398123237
343 343 g, t dbSNP:776569076
350 350 c, t dbSNP:768778881
354 354 c, t dbSNP:786205673
360 360 a, g dbSNP:747101015
371 371 a, g dbSNP:546131174
378 378 g, t dbSNP:200935970
380 380 c, g dbSNP:745604255
387 387 g, t dbSNP:778819412
393 393 c, g, t dbSNP:753883162
394 394 a, g, t dbSNP:538431094
398 398 a, g dbSNP:532399422
399 399 a, g dbSNP:767239546
408 408 c, g dbSNP:759309329
412 412 a, g dbSNP:773416756
427 427 a, g dbSNP:765699404
430 430 c, t dbSNP:762212333
438 438 a, g dbSNP:777134888
441 441 a, g dbSNP:769151272
445 445 c, t dbSNP:372083359
473 473 c, t dbSNP:748981851
474 474 a, g dbSNP:781604428
475 475 c, t dbSNP:367989163
486 486 c, t dbSNP:747403896
490 490 a, t dbSNP:200457516
495 495 c, t dbSNP:140278510
499 499 a, g dbSNP:750505590
503 503 g, t dbSNP:779171218
506 506 a, t dbSNP:757545827
508 508 c, t dbSNP:121918174
519 519 g, t dbSNP:764393614
520 520 c, g dbSNP:760324067
525 525 c, g dbSNP:752571900
526 526 a, g dbSNP:767394322
527 527 c, t dbSNP:759521075
528 528 a, g dbSNP:773822519
531 531 a, t dbSNP:201178083
536 536 c, g dbSNP:773636939
540 540 c, t dbSNP:537957979
544 544 a, g dbSNP:772672861
546 546 a, c, t dbSNP:375590538
547 547 a, g dbSNP:780513114
549 549 g, t dbSNP:772673266
557 557 c, g dbSNP:138618819
558 558 c, t dbSNP:779124500
559 559 a, g dbSNP:202210104
569 569 c, g dbSNP:753959306
570 570 c, t dbSNP:777822251
571 571 a, g, t dbSNP:373800055
584 584 a, g dbSNP:200298579
589 589 a, g dbSNP:759468015
599 599 a, g dbSNP:751438873
613 613 c, g dbSNP:764866250
624 624 g, t dbSNP:761355885
635 635 a, c dbSNP:150086524
639 639 a, t dbSNP:140709901
641 641 c, t dbSNP:200941434
643 643 c, g dbSNP:774882132
654 654 c, t dbSNP:771320756
656 656 c, t dbSNP:768075787
657 657 a, g dbSNP:373590814
659 659 c, t dbSNP:769976411
671 671 a, g dbSNP:748229629
680 680 a, c, g dbSNP:755211573
682 682 c, t dbSNP:571704452
687 687 c, t dbSNP:369465888
688 688 a, g dbSNP:150999162
703 703 c, t dbSNP:758263587
712 712 a, g dbSNP:750430817
717 717 a, g dbSNP:765097533
723 723 c, t dbSNP:779460964
725 725 c, g dbSNP:756721679
736 736 c, t dbSNP:753430922
741 741 a, g dbSNP:398123238
745 745 a, g dbSNP:377665214
746 746 a, t dbSNP:760452541
749 749 c, g dbSNP:751945330
757 757 c, t dbSNP:766837276
760 760 a, g dbSNP:763358124
761 761 c, t dbSNP:773699561
762 762 a, g dbSNP:528899475
779 779 c, t dbSNP:761828939
780 780 a, c dbSNP:776736558
782 782 c, t dbSNP:569297611
789 789 -, tcataggt dbSNP:760367429
793 793 g, t dbSNP:375828604
807 807 c, t dbSNP:371554909
808 808 a, g dbSNP:188921177
812 812 c, t dbSNP:769871779
816 816 a, c, g dbSNP:376068646
819 819 c, t dbSNP:780604522
821 821 c, g dbSNP:754650149
822 822 c, t dbSNP:746697465
825 825 c, t dbSNP:779693774
830 830 c, t dbSNP:757549474
836 836 a, g dbSNP:375970959
838 838 a, g dbSNP:142706520
841 841 c, g dbSNP:764537946
863 863 g, t dbSNP:561880652
883 883 c, t dbSNP:752792335
886 886 c, t dbSNP:767561248
895 895 a, g dbSNP:372851133
898 898 a, g dbSNP:774381911
899 899 c, g dbSNP:766716968
900 900 a, c dbSNP:368189360
900 900 -, c dbSNP:772885108
906 906 a, g dbSNP:762798994
913 913 c, t dbSNP:773187021
916 916 c, t dbSNP:141303888
917 917 a, g dbSNP:531607427
919 919 c, t dbSNP:147820162
925 925 a, c, g dbSNP:121918182
926 926 c, t dbSNP:191153460
928 928 g, t dbSNP:767992971
931 931 a, g dbSNP:200412277
936 936 c, t dbSNP:121918175
937 937 a, g dbSNP:554759648
940 940 c, t dbSNP:758070679
944 944 c, t dbSNP:121918185
958 958 c, t dbSNP:139976112
959 959 a, g dbSNP:398123234
966 966 c, t dbSNP:771629102
967 967 a, g dbSNP:759063684
971 971 c, g dbSNP:535768789
974 974 a, g dbSNP:770650835
985 985 c, g dbSNP:374490058
991 991 a, g dbSNP:778654745
995 995 c, g, t dbSNP:747572640
996 996 a, g dbSNP:780716481
1001 1001 c, t dbSNP:11559283
1009 1009 c, t dbSNP:374755881
1012 1012 a, c, g, t dbSNP:371271170
1013 1013 a, g dbSNP:377615121
1017 1017 c, t dbSNP:74410123
1019 1019 c, t dbSNP:121918173
1020 1020 c, g dbSNP:764018631
1021 1021 c, t dbSNP:756033004
1022 1022 a, c dbSNP:200451572
1032 1032 a, g dbSNP:767451327
1038 1038 a, g dbSNP:759010501
1042 1042 a, g dbSNP:757058191
1044 1044 a, g dbSNP:398123235
1057 1057 a, g dbSNP:369190724
1060 1060 g, t dbSNP:149761698
1067 1067 a, c, t dbSNP:749520560
1068 1068 a, g dbSNP:776843811
1070 1070 c, t dbSNP:376589676
1071 1071 a, g dbSNP:556424939
1087 1087 c, t dbSNP:375214425
1088 1088 a, g dbSNP:200149500
1090 1090 c, t dbSNP:745919844
1094 1094 -, c dbSNP:761247245
1097 1097 c, t dbSNP:779091113
1099 1099 c, t dbSNP:757418833
1100 1100 a, g dbSNP:749582075
1102 1102 c, t dbSNP:777871501
1103 1103 a, g dbSNP:755842317
1111 1111 a, g dbSNP:747608667
1113 1113 c, t dbSNP:752523400
1114 1114 a, g dbSNP:767396235
1115 1115 c, t dbSNP:150686327
1117 1117 c, g dbSNP:367551992
1119 1119 c, t dbSNP:751025746
1128 1128 g, t dbSNP:193920810
1131 1131 a, g dbSNP:771376085
1136 1136 a, g dbSNP:763043756
1142 1142 c, t dbSNP:773334034
1146 1146 a, g dbSNP:769976774
1150 1150 c, t dbSNP:748423878
1160 1160 a, g dbSNP:376131143
1163 1163 a, g dbSNP:768428238
1164 1164 c, t dbSNP:766849255
1166 1166 a, g dbSNP:143040697
1172 1172 a, g dbSNP:369767887
1175 1175 -, gtg dbSNP:768543128
1178 1178 c, t dbSNP:758314777
1179 1179 a, g dbSNP:749917538
1188 1188 a, g dbSNP:778631803
1194 1194 -, c dbSNP:35957142
1198 1198 c, t dbSNP:368144710
1199 1199 a, g dbSNP:753483823
1200 1200 c, t dbSNP:763845326
1204 1204 c, t dbSNP:139776224
1210 1210 a, g dbSNP:751894676
1213 1213 a, g dbSNP:121918180
1225 1225 a, c, t dbSNP:376567545
1234 1234 a, g dbSNP:118066970
1236 1236 c, g dbSNP:769803627
1244 1244 a, g dbSNP:761881997
1258 1258 c, t dbSNP:776791543
1276 1276 c, t dbSNP:764344489
1277 1277 a, g dbSNP:752854143
1280 1280 c, t dbSNP:775341321
1290 1290 c, t dbSNP:771707097
1291 1291 c, t dbSNP:138554083
1294 1294 a, g dbSNP:745662610
1304 1304 c, t dbSNP:774393243
1305 1305 a, g dbSNP:771017036
1306 1306 a, g dbSNP:748788618
1318 1318 c, t dbSNP:777191171
1329 1329 c, t dbSNP:755791810
1336 1336 c, t dbSNP:200831987
1345 1345 c, t dbSNP:143084006
1347 1347 -, a dbSNP:771250055
1355 1355 c, g dbSNP:143495981
1356 1356 c, t dbSNP:747719992
1357 1357 a, g dbSNP:548880426
1359 1359 a, g dbSNP:121918178
1372 1372 c, g dbSNP:760449944
1386 1386 a, g dbSNP:768343264
1390 1390 c, t dbSNP:746198220
1396 1396 a, g dbSNP:121918179
1401 1401 -, a dbSNP:778161730
1402 1402 a, c, t dbSNP:201928248
1403 1403 a, g dbSNP:778349139
1407 1407 a, g dbSNP:199694121
1408 1408 c, t dbSNP:752845837
1428 1428 a, c dbSNP:767826344
1436 1436 c, g dbSNP:759880257
1457 1457 c, t dbSNP:751397640
1460 1460 c, t dbSNP:767730315
1461 1461 a, g dbSNP:786205674
1462 1462 c, t dbSNP:766339888
1466 1466 -, aag dbSNP:758492902
1480 1480 c, t dbSNP:762859080
1492 1492 c, t dbSNP:377519272
1495 1495 a, g dbSNP:368053364
1498 1498 -, t dbSNP:756256226
1498 1498 c, t dbSNP:769788461
1510 1510 a, g dbSNP:761204529
1519 1519 a, g dbSNP:776242697
1528 1528 c, g dbSNP:768292111
1529 1529 c, g dbSNP:760181741
1538 1538 c, t dbSNP:775084641
1555 1555 a, g dbSNP:771735289
1562 1562 a, c dbSNP:749594772
1569 1569 g, t dbSNP:149448152
1570 1570 c, g dbSNP:770200580
1574 1574 a, g dbSNP:748750514
1600 1600 a, c dbSNP:781768877
1602 1602 a, g dbSNP:755045141
1604 1604 c, t dbSNP:747104326
1605 1605 g, t dbSNP:121918183
1613 1613 c, t dbSNP:780397637
1615 1615 c, t dbSNP:1061361
1616 1616 a, g dbSNP:150304382
1617 1617 c, t dbSNP:765136502
1621 1621 c, t dbSNP:373819742
1622 1622 a, g dbSNP:757015172
1627 1627 a, g dbSNP:141430018
1631 1631 a, t dbSNP:764050758
1645 1645 a, c, t dbSNP:200228580
1652 1652 a, g dbSNP:113059520
1654 1654 a, c, g dbSNP:564360146
1663 1663 a, g dbSNP:759301811
1666 1666 a, g dbSNP:767115386
1668 1668 c, t dbSNP:758984878
1669 1669 a, g dbSNP:751161248
1671 1671 c, t dbSNP:765983374
1672 1672 a, g dbSNP:552724524
1677 1677 g, t dbSNP:762241590
1697 1697 a, c dbSNP:777173084
1706 1706 c, t dbSNP:121918176
1720 1720 -, a dbSNP:35302712
1720 1720 a, t dbSNP:761319717
1725 1725 g, t dbSNP:775873162
1728 1728 c, g dbSNP:772122474
1731 1731 c, t dbSNP:121918172
1732 1732 a, g dbSNP:779147312
1742 1742 c, t dbSNP:751840849
1749 1749 a, g dbSNP:372550726
1751 1751 c, t dbSNP:777613366
1756 1756 g, t dbSNP:121918184
1762 1762 g, t dbSNP:756035101
1766 1766 a, c dbSNP:752576315
1767 1767 a, g dbSNP:780999350
1773 1773 c, t dbSNP:754474825
1776 1776 c, g dbSNP:751075889
1777 1777 a, g dbSNP:766019399
1780 1780 -, t dbSNP:762840002
1781 1781 c, t dbSNP:145910162
1782 1782 a, c dbSNP:762757920
1793 1793 -, tag dbSNP:770237165
1800 1800 c, t dbSNP:754238750
1804 1804 a, g dbSNP:764493374
1816 1816 -, c dbSNP:372007777
1817 1817 a, c, g dbSNP:776024156
1820 1820 c, t dbSNP:772478693
1821 1821 c, t dbSNP:9530
1821 1821 c, t dbSNP:544927524
1822 1822 a, g dbSNP:774336516
1827 1827 c, g dbSNP:557221338
1829 1829 g, t dbSNP:771138602
1835 1835 a, t dbSNP:201204553
1843 1843 a, c, g dbSNP:769514976
1844 1844 a, c dbSNP:747928978
1849 1849 -, t dbSNP:769662185
1851 1851 c, t dbSNP:150311243
1852 1852 a, g dbSNP:754908402
1854 1854 a, g dbSNP:751073317
1856 1856 c, t dbSNP:779485917
1865 1865 c, g dbSNP:62470933
1868 1868 a, g dbSNP:757993425
1872 1872 c, g, t dbSNP:765012426
1877 1877 c, t dbSNP:756497692
1881 1881 a, t dbSNP:753051726
1886 1886 c, t dbSNP:372605666
1900 1900 c, g dbSNP:753596986
1918 1918 a, g dbSNP:567259021
1925 1925 a, g dbSNP:536903750
1979 1979 a, g dbSNP:568655640
1992 1992 a, g dbSNP:140236850
2003 2003 -, a dbSNP:762261099
2003 2003 a, g dbSNP:77293332
2025 2025 a, g dbSNP:138519677
2039 2039 g, t dbSNP:190201470

Target ORF information:

RefSeq Version XM_011516113
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens glucuronidase, beta (GUSB), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011516113

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGACCCAG GACCTGCGCA CAAGAGTGGT GCTGAGGATT GGCAGTGCCC ATTCCTATGC 
CATCGTGGTA TCCCAAGGGT TACTTTGTCC AGAACACATA TTTTGACTTT TTCAACTACG
CTGGACTGCA GCGGTCTGTA CTTCTGTACA CGACACCCAC CACCTACATC GATGACATCA
CCGTCACCAC CAGCGTGGAG CAAGACAGTG GTGAGGGCTT CTGGGCTGGT GAATTACCAG
ATCTCTGTCA AGGGCAGTAA CCTGTTCAAG TTGGAAGTGC GTCTTTTGGA TGCAGAAAAC
AAAGTCGTGG CGAATGGGAC TGGGACCCAG GGCCAACTTA AGGTGCCAGG TGTCAGCCTC
TGGTGGCCGT ACCTGATGCA CGAACGCCCT GCCTATCTGT ATTCATTGGA GGTGCAGCTG
ACTGCACAGA CGTCACTGGG GCCTGTGTCT GACTTCTACA CACTCCCTGT GGGGATCCGC
ACTGTGGCTG TCACCAAGAG CCAGTTCCTC ATCAATGGGA AACCTTTCTA TTTCCACGGT
GTCAACAAGC ATGAGGATGC GGACATCCGA GGGAAGGGCT TCGACTGGCC GCTGCTGGTG
AAGGACTTCA ACCTGCTTCG CTGGCTTGGT GCCAACGCTT TCCGTACCAG CCACTACCCC
TATGCAGAGG AAGTGATGCA GATGTGTGAC CGCTATGGGA TTGTGGTCAT CGATGAGTGT
CCCGGCGTGG GCCTGGCGCT GCCGCAGTTC TTCAACAACG TTTCTCTGCA TCACCACATG
CAGGTGATGG AAGAAGTGGT GCGTAGGGAC AAGAACCACC CCGCGGTCGT GATGTGGTCT
GTGGCCAACG AGCCTGCGTC CCACCTAGAA TCTGCTGGCT ACTACTTGAA GATGGTGATC
GCTCACACCA AATCCTTGGA CCCCTCCCGG CCTGTGACCT TTGTGAGCAA CTCTAACTAT
GCAGCAGACA AGGGGGCTCC GTATGTGGAT GTGATCTGTT TGAACAGCTA CTACTCTTGG
TATCACGACT ACGGGCACCT GGAGTTGATT CAGCTGCAGC TGGCCACCCA GTTTGAGAAC
TGGTATAAGA AGTATCAGAA GCCCATTATT CAGAGCGAGT ATGGAGCAGA AACGATTGCA
GGGTTTCACC AGGATCCACC TCTGATGTTC ACTGAAGAGT ACCAGAAAAG TCTGCTAGAG
CAGTACCATC TGGGTCTGGA TCAAAAACGC AGAAAATACG TGGTTGGAGA GCTCATTTGG
AATTTTGCCG ATTTCATGAC TGAACAGTCA CCGACGAGAG TGCTGGGGAA TAAAAAGGGG
ATCTTCACTC GGCAGAGACA ACCAAAAAGT GCAGCGTTCC TTTTGCGAGA GAGATACTGG
AAGATTGCCA ATGAAACCAG GTATCCCCAC TCAGTAGCCA AGTCACAATG TTTGGAAAAC
AGCCTGTTTA CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58166
Accession Version XM_011516114.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product beta-glucuronidase isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACATCAC CGTCACCACC AGCGTGGAGC AAGACAGTGG TGAGGGCTTC TGGGCTGGTG 
AATTACCAGA TCTCTGTCAA GGGCAGTAAC CTGTTCAAGT TGGAAGTGCG TCTTTTGGAT
GCAGAAAACA AAGTCGTGGC GAATGGGACT GGGACCCAGG GCCAACTTAA GGTGCCAGGT
GTCAGCCTCT GGTGGCCGTA CCTGATGCAC GAACGCCCTG CCTATCTGTA TTCATTGGAG
GTGCAGCTGA CTGCACAGAC GTCACTGGGG CCTGTGTCTG ACTTCTACAC ACTCCCTGTG
GGGATCCGCA CTGTGGCTGT CACCAAGAGC CAGTTCCTCA TCAATGGGAA ACCTTTCTAT
TTCCACGGTG TCAACAAGCA TGAGGATGCG GACATCCGAG GGAAGGGCTT CGACTGGCCG
CTGCTGGTGA AGGACTTCAA CCTGCTTCGC TGGCTTGGTG CCAACGCTTT CCGTACCAGC
CACTACCCCT ATGCAGAGGA AGTGATGCAG ATGTGTGACC GCTATGGGAT TGTGGTCATC
GATGAGTGTC CCGGCGTGGG CCTGGCGCTG CCGCAGTTCT TCAACAACGT TTCTCTGCAT
CACCACATGC AGGTGATGGA AGAAGTGGTG CGTAGGGACA AGAACCACCC CGCGGTCGTG
ATGTGGTCTG TGGCCAACGA GCCTGCGTCC CACCTAGAAT CTGCTGGCTA CTACTTGAAG
ATGGTGATCG CTCACACCAA ATCCTTGGAC CCCTCCCGGC CTGTGACCTT TGTGAGCAAC
TCTAACTATG CAGCAGACAA GGGGGCTCCG TATGTGGATG TGATCTGTTT GAACAGCTAC
TACTCTTGGT ATCACGACTA CGGGCACCTG GAGTTGATTC AGCTGCAGCT GGCCACCCAG
TTTGAGAACT GGTATAAGAA GTATCAGAAG CCCATTATTC AGAGCGAGTA TGGAGCAGAA
ACGATTGCAG GGTTTCACCA GGATCCACCT CTGATGTTCA CTGAAGAGTA CCAGAAAAGT
CTGCTAGAGC AGTACCATCT GGGTCTGGAT CAAAAACGCA GAAAATACGT GGTTGGAGAG
CTCATTTGGA ATTTTGCCGA TTTCATGACT GAACAGTCAC CGACGAGAGT GCTGGGGAAT
AAAAAGGGGA TCTTCACTCG GCAGAGACAA CCAAAAAGTG CAGCGTTCCT TTTGCGAGAG
AGATACTGGA AGATTGCCAA TGAAACCAGG TATCCCCACT CAGTAGCCAA GTCACAATGT
TTGGAAAACA GCCTGTTTAC TTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011514416.1
CDS476..1759
Misc Feature(1)788..1696(+)
Translation
Position Chain Variation Link
6 6 c, t dbSNP:77774169
7 7 a, c dbSNP:748127836
12 12 c, g, t dbSNP:140016611
20 20 g, t dbSNP:751845855
22 22 c, g dbSNP:779807872
23 23 c, t dbSNP:758127615
39 39 c, t dbSNP:750221073
41 41 a, g dbSNP:765042077
49 49 a, g dbSNP:757243831
50 50 g, t dbSNP:753270240
51 51 c, t dbSNP:112435030
56 56 a, g dbSNP:398123236
61 61 c, t dbSNP:763704693
62 62 a, g dbSNP:147454954
67 67 c, t dbSNP:769047951
68 68 a, g dbSNP:767292825
76 76 g, t dbSNP:763344202
78 78 c, t dbSNP:773646634
79 79 a, g dbSNP:770176365
80 80 a, g dbSNP:748729882
85 85 a, g dbSNP:398123237
86 86 g, t dbSNP:776569076
93 93 c, t dbSNP:768778881
97 97 c, t dbSNP:786205673
103 103 a, g dbSNP:747101015
114 114 a, g dbSNP:546131174
121 121 g, t dbSNP:200935970
123 123 c, g dbSNP:745604255
130 130 g, t dbSNP:778819412
136 136 c, g, t dbSNP:753883162
137 137 a, g, t dbSNP:538431094
141 141 a, g dbSNP:532399422
142 142 a, g dbSNP:767239546
151 151 c, g dbSNP:759309329
155 155 a, g dbSNP:773416756
170 170 a, g dbSNP:765699404
173 173 c, t dbSNP:762212333
181 181 a, g dbSNP:777134888
184 184 a, g dbSNP:769151272
188 188 c, g dbSNP:786205671
197 197 c, g dbSNP:749073290
201 201 c, t dbSNP:141441283
202 202 a, c, g dbSNP:748161388
206 206 c, t dbSNP:780835314
210 210 a, c dbSNP:754452177
219 219 a, c, g, t dbSNP:375740757
232 232 c, t dbSNP:121918177
237 237 c, t dbSNP:148364905
240 240 c, g dbSNP:764486500
243 243 c, g, t dbSNP:144397476
244 244 a, g dbSNP:149606212
282 282 c, t dbSNP:759593913
283 283 c, g, t dbSNP:771137253
288 288 c, g, t dbSNP:773134072
289 289 c, t dbSNP:769769379
292 292 a, g dbSNP:747969208
298 298 a, g dbSNP:781205550
300 300 c, t dbSNP:752674900
301 301 a, g dbSNP:746475728
304 304 a, g dbSNP:369537067
309 309 c, t dbSNP:779475930
316 316 c, t dbSNP:121918181
317 317 -, tcac dbSNP:776287966
320 320 c, t dbSNP:587779400
322 322 a, c dbSNP:11559281
323 323 c, t dbSNP:778102597
330 330 c, t dbSNP:138435796
335 335 c, t dbSNP:756417262
338 338 c, t dbSNP:753065037
342 342 a, g dbSNP:768029322
343 343 a, c dbSNP:755465262
350 350 a, g dbSNP:751638113
351 351 a, g dbSNP:74430256
360 360 c, t dbSNP:763253448
373 373 c, t dbSNP:372083359
401 401 c, t dbSNP:748981851
402 402 a, g dbSNP:781604428
403 403 c, t dbSNP:367989163
414 414 c, t dbSNP:747403896
418 418 a, t dbSNP:200457516
423 423 c, t dbSNP:140278510
427 427 a, g dbSNP:750505590
431 431 g, t dbSNP:779171218
434 434 a, t dbSNP:757545827
436 436 c, t dbSNP:121918174
447 447 g, t dbSNP:764393614
448 448 c, g dbSNP:760324067
453 453 c, g dbSNP:752571900
454 454 a, g dbSNP:767394322
455 455 c, t dbSNP:759521075
456 456 a, g dbSNP:773822519
459 459 a, t dbSNP:201178083
464 464 c, g dbSNP:773636939
468 468 c, t dbSNP:537957979
472 472 a, g dbSNP:772672861
474 474 a, c, t dbSNP:375590538
475 475 a, g dbSNP:780513114
477 477 g, t dbSNP:772673266
485 485 c, g dbSNP:138618819
486 486 c, t dbSNP:779124500
487 487 a, g dbSNP:202210104
497 497 c, g dbSNP:753959306
498 498 c, t dbSNP:777822251
499 499 a, g, t dbSNP:373800055
512 512 a, g dbSNP:200298579
517 517 a, g dbSNP:759468015
527 527 a, g dbSNP:751438873
541 541 c, g dbSNP:764866250
552 552 g, t dbSNP:761355885
563 563 a, c dbSNP:150086524
567 567 a, t dbSNP:140709901
569 569 c, t dbSNP:200941434
571 571 c, g dbSNP:774882132
582 582 c, t dbSNP:771320756
584 584 c, t dbSNP:768075787
585 585 a, g dbSNP:373590814
587 587 c, t dbSNP:769976411
599 599 a, g dbSNP:748229629
608 608 a, c, g dbSNP:755211573
610 610 c, t dbSNP:571704452
615 615 c, t dbSNP:369465888
616 616 a, g dbSNP:150999162
631 631 c, t dbSNP:758263587
640 640 a, g dbSNP:750430817
645 645 a, g dbSNP:765097533
651 651 c, t dbSNP:779460964
653 653 c, g dbSNP:756721679
664 664 c, t dbSNP:753430922
669 669 a, g dbSNP:398123238
673 673 a, g dbSNP:377665214
674 674 a, t dbSNP:760452541
677 677 c, g dbSNP:751945330
685 685 c, t dbSNP:766837276
688 688 a, g dbSNP:763358124
689 689 c, t dbSNP:773699561
690 690 a, g dbSNP:528899475
707 707 c, t dbSNP:761828939
708 708 a, c dbSNP:776736558
710 710 c, t dbSNP:569297611
717 717 -, tcataggt dbSNP:760367429
721 721 g, t dbSNP:375828604
735 735 c, t dbSNP:371554909
736 736 a, g dbSNP:188921177
740 740 c, t dbSNP:769871779
744 744 a, c, g dbSNP:376068646
747 747 c, t dbSNP:780604522
749 749 c, g dbSNP:754650149
750 750 c, t dbSNP:746697465
753 753 c, t dbSNP:779693774
758 758 c, t dbSNP:757549474
764 764 a, g dbSNP:375970959
766 766 a, g dbSNP:142706520
769 769 c, g dbSNP:764537946
791 791 g, t dbSNP:561880652
811 811 c, t dbSNP:752792335
814 814 c, t dbSNP:767561248
823 823 a, g dbSNP:372851133
826 826 a, g dbSNP:774381911
827 827 c, g dbSNP:766716968
828 828 a, c dbSNP:368189360
828 828 -, c dbSNP:772885108
834 834 a, g dbSNP:762798994
841 841 c, t dbSNP:773187021
844 844 c, t dbSNP:141303888
845 845 a, g dbSNP:531607427
847 847 c, t dbSNP:147820162
853 853 a, c, g dbSNP:121918182
854 854 c, t dbSNP:191153460
856 856 g, t dbSNP:767992971
859 859 a, g dbSNP:200412277
864 864 c, t dbSNP:121918175
865 865 a, g dbSNP:554759648
868 868 c, t dbSNP:758070679
872 872 c, t dbSNP:121918185
886 886 c, t dbSNP:139976112
887 887 a, g dbSNP:398123234
894 894 c, t dbSNP:771629102
895 895 a, g dbSNP:759063684
899 899 c, g dbSNP:535768789
902 902 a, g dbSNP:770650835
913 913 c, g dbSNP:374490058
919 919 a, g dbSNP:778654745
923 923 c, g, t dbSNP:747572640
924 924 a, g dbSNP:780716481
929 929 c, t dbSNP:11559283
937 937 c, t dbSNP:374755881
940 940 a, c, g, t dbSNP:371271170
941 941 a, g dbSNP:377615121
945 945 c, t dbSNP:74410123
947 947 c, t dbSNP:121918173
948 948 c, g dbSNP:764018631
949 949 c, t dbSNP:756033004
950 950 a, c dbSNP:200451572
960 960 a, g dbSNP:767451327
966 966 a, g dbSNP:759010501
970 970 a, g dbSNP:757058191
972 972 a, g dbSNP:398123235
985 985 a, g dbSNP:369190724
988 988 g, t dbSNP:149761698
995 995 a, c, t dbSNP:749520560
996 996 a, g dbSNP:776843811
998 998 c, t dbSNP:376589676
999 999 a, g dbSNP:556424939
1015 1015 c, t dbSNP:375214425
1016 1016 a, g dbSNP:200149500
1018 1018 c, t dbSNP:745919844
1022 1022 -, c dbSNP:761247245
1025 1025 c, t dbSNP:779091113
1027 1027 c, t dbSNP:757418833
1028 1028 a, g dbSNP:749582075
1030 1030 c, t dbSNP:777871501
1031 1031 a, g dbSNP:755842317
1039 1039 a, g dbSNP:747608667
1041 1041 c, t dbSNP:752523400
1042 1042 a, g dbSNP:767396235
1043 1043 c, t dbSNP:150686327
1045 1045 c, g dbSNP:367551992
1047 1047 c, t dbSNP:751025746
1056 1056 g, t dbSNP:193920810
1059 1059 a, g dbSNP:771376085
1064 1064 a, g dbSNP:763043756
1070 1070 c, t dbSNP:773334034
1074 1074 a, g dbSNP:769976774
1078 1078 c, t dbSNP:748423878
1088 1088 a, g dbSNP:376131143
1091 1091 a, g dbSNP:768428238
1092 1092 c, t dbSNP:766849255
1094 1094 a, g dbSNP:143040697
1100 1100 a, g dbSNP:369767887
1103 1103 -, gtg dbSNP:768543128
1106 1106 c, t dbSNP:758314777
1107 1107 a, g dbSNP:749917538
1116 1116 a, g dbSNP:778631803
1122 1122 -, c dbSNP:35957142
1126 1126 c, t dbSNP:368144710
1127 1127 a, g dbSNP:753483823
1128 1128 c, t dbSNP:763845326
1132 1132 c, t dbSNP:139776224
1138 1138 a, g dbSNP:751894676
1141 1141 a, g dbSNP:121918180
1153 1153 a, c, t dbSNP:376567545
1162 1162 a, g dbSNP:118066970
1164 1164 c, g dbSNP:769803627
1172 1172 a, g dbSNP:761881997
1186 1186 c, t dbSNP:776791543
1204 1204 c, t dbSNP:764344489
1205 1205 a, g dbSNP:752854143
1208 1208 c, t dbSNP:775341321
1218 1218 c, t dbSNP:771707097
1219 1219 c, t dbSNP:138554083
1222 1222 a, g dbSNP:745662610
1232 1232 c, t dbSNP:774393243
1233 1233 a, g dbSNP:771017036
1234 1234 a, g dbSNP:748788618
1246 1246 c, t dbSNP:777191171
1257 1257 c, t dbSNP:755791810
1264 1264 c, t dbSNP:200831987
1273 1273 c, t dbSNP:143084006
1275 1275 -, a dbSNP:771250055
1283 1283 c, g dbSNP:143495981
1284 1284 c, t dbSNP:747719992
1285 1285 a, g dbSNP:548880426
1287 1287 a, g dbSNP:121918178
1300 1300 c, g dbSNP:760449944
1314 1314 a, g dbSNP:768343264
1318 1318 c, t dbSNP:746198220
1324 1324 a, g dbSNP:121918179
1329 1329 -, a dbSNP:778161730
1330 1330 a, c, t dbSNP:201928248
1331 1331 a, g dbSNP:778349139
1335 1335 a, g dbSNP:199694121
1336 1336 c, t dbSNP:752845837
1356 1356 a, c dbSNP:767826344
1364 1364 c, g dbSNP:759880257
1385 1385 c, t dbSNP:751397640
1388 1388 c, t dbSNP:767730315
1389 1389 a, g dbSNP:786205674
1390 1390 c, t dbSNP:766339888
1394 1394 -, aag dbSNP:758492902
1408 1408 c, t dbSNP:762859080
1420 1420 c, t dbSNP:377519272
1423 1423 a, g dbSNP:368053364
1426 1426 -, t dbSNP:756256226
1426 1426 c, t dbSNP:769788461
1438 1438 a, g dbSNP:761204529
1447 1447 a, g dbSNP:776242697
1456 1456 c, g dbSNP:768292111
1457 1457 c, g dbSNP:760181741
1466 1466 c, t dbSNP:775084641
1483 1483 a, g dbSNP:771735289
1490 1490 a, c dbSNP:749594772
1497 1497 g, t dbSNP:149448152
1498 1498 c, g dbSNP:770200580
1502 1502 a, g dbSNP:748750514
1528 1528 a, c dbSNP:781768877
1530 1530 a, g dbSNP:755045141
1532 1532 c, t dbSNP:747104326
1533 1533 g, t dbSNP:121918183
1541 1541 c, t dbSNP:780397637
1543 1543 c, t dbSNP:1061361
1544 1544 a, g dbSNP:150304382
1545 1545 c, t dbSNP:765136502
1549 1549 c, t dbSNP:373819742
1550 1550 a, g dbSNP:757015172
1555 1555 a, g dbSNP:141430018
1559 1559 a, t dbSNP:764050758
1573 1573 a, c, t dbSNP:200228580
1580 1580 a, g dbSNP:113059520
1582 1582 a, c, g dbSNP:564360146
1591 1591 a, g dbSNP:759301811
1594 1594 a, g dbSNP:767115386
1596 1596 c, t dbSNP:758984878
1597 1597 a, g dbSNP:751161248
1599 1599 c, t dbSNP:765983374
1600 1600 a, g dbSNP:552724524
1605 1605 g, t dbSNP:762241590
1625 1625 a, c dbSNP:777173084
1634 1634 c, t dbSNP:121918176
1648 1648 -, a dbSNP:35302712
1648 1648 a, t dbSNP:761319717
1653 1653 g, t dbSNP:775873162
1656 1656 c, g dbSNP:772122474
1659 1659 c, t dbSNP:121918172
1660 1660 a, g dbSNP:779147312
1670 1670 c, t dbSNP:751840849
1677 1677 a, g dbSNP:372550726
1679 1679 c, t dbSNP:777613366
1684 1684 g, t dbSNP:121918184
1690 1690 g, t dbSNP:756035101
1694 1694 a, c dbSNP:752576315
1695 1695 a, g dbSNP:780999350
1701 1701 c, t dbSNP:754474825
1704 1704 c, g dbSNP:751075889
1705 1705 a, g dbSNP:766019399
1708 1708 -, t dbSNP:762840002
1709 1709 c, t dbSNP:145910162
1710 1710 a, c dbSNP:762757920
1721 1721 -, tag dbSNP:770237165
1728 1728 c, t dbSNP:754238750
1732 1732 a, g dbSNP:764493374
1744 1744 -, c dbSNP:372007777
1745 1745 a, c, g dbSNP:776024156
1748 1748 c, t dbSNP:772478693
1749 1749 c, t dbSNP:9530
1749 1749 c, t dbSNP:544927524
1750 1750 a, g dbSNP:774336516
1755 1755 c, g dbSNP:557221338
1757 1757 g, t dbSNP:771138602
1763 1763 a, t dbSNP:201204553
1771 1771 a, c, g dbSNP:769514976
1772 1772 a, c dbSNP:747928978
1777 1777 -, t dbSNP:769662185
1779 1779 c, t dbSNP:150311243
1780 1780 a, g dbSNP:754908402
1782 1782 a, g dbSNP:751073317
1784 1784 c, t dbSNP:779485917
1793 1793 c, g dbSNP:62470933
1796 1796 a, g dbSNP:757993425
1800 1800 c, g, t dbSNP:765012426
1805 1805 c, t dbSNP:756497692
1809 1809 a, t dbSNP:753051726
1814 1814 c, t dbSNP:372605666
1828 1828 c, g dbSNP:753596986
1846 1846 a, g dbSNP:567259021
1853 1853 a, g dbSNP:536903750
1907 1907 a, g dbSNP:568655640
1920 1920 a, g dbSNP:140236850
1931 1931 -, a dbSNP:762261099
1931 1931 a, g dbSNP:77293332
1953 1953 a, g dbSNP:138519677
1967 1967 g, t dbSNP:190201470

Target ORF information:

RefSeq Version XM_011516114
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens glucuronidase, beta (GUSB), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011516114

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACATCAC CGTCACCACC AGCGTGGAGC AAGACAGTGG TGAGGGCTTC TGGGCTGGTG 
AATTACCAGA TCTCTGTCAA GGGCAGTAAC CTGTTCAAGT TGGAAGTGCG TCTTTTGGAT
GCAGAAAACA AAGTCGTGGC GAATGGGACT GGGACCCAGG GCCAACTTAA GGTGCCAGGT
GTCAGCCTCT GGTGGCCGTA CCTGATGCAC GAACGCCCTG CCTATCTGTA TTCATTGGAG
GTGCAGCTGA CTGCACAGAC GTCACTGGGG CCTGTGTCTG ACTTCTACAC ACTCCCTGTG
GGGATCCGCA CTGTGGCTGT CACCAAGAGC CAGTTCCTCA TCAATGGGAA ACCTTTCTAT
TTCCACGGTG TCAACAAGCA TGAGGATGCG GACATCCGAG GGAAGGGCTT CGACTGGCCG
CTGCTGGTGA AGGACTTCAA CCTGCTTCGC TGGCTTGGTG CCAACGCTTT CCGTACCAGC
CACTACCCCT ATGCAGAGGA AGTGATGCAG ATGTGTGACC GCTATGGGAT TGTGGTCATC
GATGAGTGTC CCGGCGTGGG CCTGGCGCTG CCGCAGTTCT TCAACAACGT TTCTCTGCAT
CACCACATGC AGGTGATGGA AGAAGTGGTG CGTAGGGACA AGAACCACCC CGCGGTCGTG
ATGTGGTCTG TGGCCAACGA GCCTGCGTCC CACCTAGAAT CTGCTGGCTA CTACTTGAAG
ATGGTGATCG CTCACACCAA ATCCTTGGAC CCCTCCCGGC CTGTGACCTT TGTGAGCAAC
TCTAACTATG CAGCAGACAA GGGGGCTCCG TATGTGGATG TGATCTGTTT GAACAGCTAC
TACTCTTGGT ATCACGACTA CGGGCACCTG GAGTTGATTC AGCTGCAGCT GGCCACCCAG
TTTGAGAACT GGTATAAGAA GTATCAGAAG CCCATTATTC AGAGCGAGTA TGGAGCAGAA
ACGATTGCAG GGTTTCACCA GGATCCACCT CTGATGTTCA CTGAAGAGTA CCAGAAAAGT
CTGCTAGAGC AGTACCATCT GGGTCTGGAT CAAAAACGCA GAAAATACGT GGTTGGAGAG
CTCATTTGGA ATTTTGCCGA TTTCATGACT GAACAGTCAC CGACGAGAGT GCTGGGGAAT
AAAAAGGGGA TCTTCACTCG GCAGAGACAA CCAAAAAGTG CAGCGTTCCT TTTGCGAGAG
AGATACTGGA AGATTGCCAA TGAAACCAGG TATCCCCACT CAGTAGCCAA GTCACAATGT
TTGGAAAACA GCCTGTTTAC TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27736
Accession Version NM_001284290.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1518bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product beta-glucuronidase isoform 2 precursor
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC346632.1, AK303819.1, AC073261.8 and AA947121.1. On Sep 27, 2013 this sequence version replaced gi:530385801. Summary: This gene encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. The enzyme forms a homotetramer that is localized to the lysosome. Mutations in this gene result in mucopolysaccharidosis type VII. Alternative splicing results in multiple transcript variants. There are many pseudogenes of this locus in the human genome.[provided by RefSeq, May 2014]. Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK303819.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2162568 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCCGGG GGTCGGCGGT TGCCTGGGCG GCGCTCGGGC CGTTGTTGTG GGGCTGCGCG 
CTGGGGCTGC AGGGCGGGAT GCTGTACCCC CAGGAGAGCC CGTCGCGGGA GTGCAAGGAG
CTGGACGGCC TCTGGAGCTT CCGCGCCGAC TTCTCTGACA ACCGACGCCG GGGCTTCGAG
GAGCAGTGGT ACCGGCGGCC GCTGTGGGAG TCAGGCCCCA CCGTGGACAT GCCAGTTCCC
TCCAGCTTCA ATGACATCAG CCAGGACTGG CGTCTGCGGC ATTTTGTCGG CTGGGTGTGG
TACGAACGGG AGGTGATCCT GCCGGAGCGA TGGACCCAGG ACCTGCGCAC AAGAGTGGTG
CTGAGGATTG GCAGTGCCCA TTCCTATGCC ATCGTGTGGG TGAATGGGGT CGACACGCTA
GAGCATGAGG GGGGCTACCT CCCCTTCGAG GCCGACATCA GCAACCTGGT CCAGGTGCAG
CTGACTGCAC AGACGTCACT GGGGCCTGTG TCTGACTTCT ACACACTCCC TGTGGGGATC
CGCACTGTGG CTGTCACCAA GAGCCAGTTC CTCATCAATG GGAAACCTTT CTATTTCCAC
GGTGTCAACA AGCATGAGGA TGCGGACATC CGAGGGAAGG GCTTCGACTG GCCGCTGCTG
GTGAAGGACT TCAACCTGCT TCGCTGGCTT GGTGCCAACG CTTTCCGTAC CAGCCACTAC
CCCTATGCAG AGGAAGTGAT GCAGATGTGT GACCGCTATG GGATTGTGGT CATCGATGAG
TGTCCCGGCG TGGGCCTGGC GCTGCCGCAG TTCTTCAACA ACGTTTCTCT GCATCACCAC
ATGCAGGTGA TGGAAGAAGT GGTGCGTAGG GACAAGAACC ACCCCGCGGT CGTGATGTGG
TCTGTGGCCA ACGAGCCTGC GTCCCACCTA GAATCTGCTG GCTACTACTT GAAGATGGTG
ATCGCTCACA CCAAATCCTT GGACCCCTCC CGGCCTGTGA CCTTTGTGAG CAACTCTAAC
TATGCAGCAG ACAAGGGGGC TCCGTATGTG GATGTGATCT GTTTGAACAG CTACTACTCT
TGGTATCACG ACTACGGGCA CCTGGAGTTG ATTCAGCTGC AGCTGGCCAC CCAGTTTGAG
AACTGGTATA AGAAGTATCA GAAGCCCATT ATTCAGAGCG AGTATGGAGC AGAAACGATT
GCAGGGTTTC ACCAGGATCC ACCTCTGATG TTCACTGAAG AGTACCAGAA AAGTCTGCTA
GAGCAGTACC ATCTGGGTCT GGATCAAAAA CGCAGAAAAT ACGTGGTTGG AGAGCTCATT
TGGAATTTTG CCGATTTCAT GACTGAACAG TCACCGACGA GAGTGCTGGG GAATAAAAAG
GGGATCTTCA CTCGGCAGAG ACAACCAAAA AGTGCAGCGT TCCTTTTGCG AGAGAGATAC
TGGAAGATTG CCAATGAAAC CAGGTATCCC CACTCAGTAG CCAAGTCACA ATGTTTGGAA
AACAGCCTGT TTACTTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001271219.1
CDS132..1649
Misc Feature(1)93..95(+)
Misc Feature(2)210..1586(+)
Misc Feature(3)234..>605(+)
Misc Feature(4)678..1586(+)
Exon (1)1..341
Gene:GUSB
Gene Synonym:
Exon (2)342..527
Gene:GUSB
Gene Synonym:
Exon (3)528..605
Gene:GUSB
Gene Synonym:
Exon (4)606..758
Gene:GUSB
Gene Synonym:
Exon (5)759..937
Gene:GUSB
Gene Synonym:
Exon (6)938..1084
Gene:GUSB
Gene Synonym:
Exon (7)1085..1169
Gene:GUSB
Gene Synonym:
Exon (8)1170..1346
Gene:GUSB
Gene Synonym:
Exon (9)1347..1482
Gene:GUSB
Gene Synonym:
Exon (10)1483..1862
Gene:GUSB
Gene Synonym:
Translation
Position Chain Variation Link
20 20 c, g dbSNP:549392182
37 37 a, g dbSNP:748697743
53 53 g, t dbSNP:528932289
60 60 g, t dbSNP:2279904
80 80 c, g dbSNP:777048370
82 82 c, t dbSNP:201504866
90 90 g, t dbSNP:199591754
99 99 c, g dbSNP:760455943
112 112 c, g, t dbSNP:577269089
113 113 c, g dbSNP:771999951
116 116 c, t dbSNP:745843396
117 117 c, g dbSNP:781149654
120 120 a, g dbSNP:2279903
122 122 a, t dbSNP:540793200
125 125 a, g dbSNP:777339764
130 130 g, t dbSNP:755795388
131 131 c, t dbSNP:747348554
136 136 c, t dbSNP:780330659
137 137 c, g dbSNP:758903707
138 138 c, t dbSNP:750955623
139 139 g, t dbSNP:765390811
140 140 g, t dbSNP:757357472
141 141 c, g dbSNP:572131903
143 143 a, g dbSNP:753976305
144 144 c, t dbSNP:190496263
145 145 c, t dbSNP:535382125
147 147 a, g dbSNP:775335168
153 153 g, t dbSNP:376505707
185 185 c, t dbSNP:759514074
188 188 c, g, t dbSNP:573989102
215 215 a, g dbSNP:748756976
217 217 a, g dbSNP:772729596
219 219 a, c, t dbSNP:747792546
221 221 c, t dbSNP:745503914
231 231 c, t dbSNP:780459919
236 236 c, g dbSNP:758743865
242 242 g, t dbSNP:746254536
243 243 c, t dbSNP:779499448
249 249 a, g dbSNP:757874872
255 255 g, t dbSNP:753922918
269 269 c, g dbSNP:764321712
278 278 c, t dbSNP:375940373
281 281 c, t dbSNP:752931066
288 288 a, g dbSNP:371314015
292 292 a, g dbSNP:557347158
293 293 c, t dbSNP:74975849
297 297 c, t dbSNP:147262894
300 300 c, g, t dbSNP:772676579
301 301 g, t dbSNP:769252159
302 302 a, g dbSNP:567066124
316 316 a, g dbSNP:571736757
327 327 c, t dbSNP:776085845
328 328 g, t dbSNP:768362767
332 332 g, t dbSNP:551892631
333 333 c, t dbSNP:746199104
347 347 c, t dbSNP:77774169
348 348 a, c dbSNP:748127836
353 353 c, g, t dbSNP:140016611
361 361 g, t dbSNP:751845855
363 363 c, g dbSNP:779807872
364 364 c, t dbSNP:758127615
380 380 c, t dbSNP:750221073
382 382 a, g dbSNP:765042077
390 390 a, g dbSNP:757243831
391 391 g, t dbSNP:753270240
392 392 c, t dbSNP:112435030
397 397 a, g dbSNP:398123236
402 402 c, t dbSNP:763704693
403 403 a, g dbSNP:147454954
408 408 c, t dbSNP:769047951
409 409 a, g dbSNP:767292825
417 417 g, t dbSNP:763344202
419 419 c, t dbSNP:773646634
420 420 a, g dbSNP:770176365
421 421 a, g dbSNP:748729882
426 426 a, g dbSNP:398123237
427 427 g, t dbSNP:776569076
434 434 c, t dbSNP:768778881
438 438 c, t dbSNP:786205673
444 444 a, g dbSNP:747101015
455 455 a, g dbSNP:546131174
462 462 g, t dbSNP:200935970
464 464 c, g dbSNP:745604255
471 471 g, t dbSNP:778819412
477 477 c, g, t dbSNP:753883162
478 478 a, g, t dbSNP:538431094
482 482 a, g dbSNP:532399422
483 483 a, g dbSNP:767239546
492 492 c, g dbSNP:759309329
496 496 a, g dbSNP:773416756
511 511 a, g dbSNP:765699404
514 514 c, t dbSNP:762212333
522 522 a, g dbSNP:777134888
525 525 a, g dbSNP:769151272
529 529 c, g dbSNP:786205671
538 538 c, g dbSNP:749073290
542 542 c, t dbSNP:141441283
543 543 a, c, g dbSNP:748161388
547 547 c, t dbSNP:780835314
551 551 a, c dbSNP:754452177
560 560 a, c, g, t dbSNP:375740757
573 573 c, t dbSNP:121918177
578 578 c, t dbSNP:148364905
581 581 c, g dbSNP:764486500
584 584 c, g, t dbSNP:144397476
585 585 a, g dbSNP:149606212
607 607 -, tcataggt dbSNP:760367429
611 611 g, t dbSNP:375828604
625 625 c, t dbSNP:371554909
626 626 a, g dbSNP:188921177
630 630 c, t dbSNP:769871779
634 634 a, c, g dbSNP:376068646
637 637 c, t dbSNP:780604522
639 639 c, g dbSNP:754650149
640 640 c, t dbSNP:746697465
643 643 c, t dbSNP:779693774
648 648 c, t dbSNP:757549474
654 654 a, g dbSNP:375970959
656 656 a, g dbSNP:142706520
659 659 c, g dbSNP:764537946
681 681 g, t dbSNP:561880652
701 701 c, t dbSNP:752792335
704 704 c, t dbSNP:767561248
713 713 a, g dbSNP:372851133
716 716 a, g dbSNP:774381911
717 717 c, g dbSNP:766716968
718 718 a, c dbSNP:368189360
718 718 -, c dbSNP:772885108
724 724 a, g dbSNP:762798994
731 731 c, t dbSNP:773187021
734 734 c, t dbSNP:141303888
735 735 a, g dbSNP:531607427
737 737 c, t dbSNP:147820162
743 743 a, c, g dbSNP:121918182
744 744 c, t dbSNP:191153460
746 746 g, t dbSNP:767992971
749 749 a, g dbSNP:200412277
754 754 c, t dbSNP:121918175
755 755 a, g dbSNP:554759648
758 758 c, t dbSNP:758070679
762 762 c, t dbSNP:121918185
776 776 c, t dbSNP:139976112
777 777 a, g dbSNP:398123234
784 784 c, t dbSNP:771629102
785 785 a, g dbSNP:759063684
789 789 c, g dbSNP:535768789
792 792 a, g dbSNP:770650835
803 803 c, g dbSNP:374490058
809 809 a, g dbSNP:778654745
813 813 c, g, t dbSNP:747572640
814 814 a, g dbSNP:780716481
819 819 c, t dbSNP:11559283
827 827 c, t dbSNP:374755881
830 830 a, c, g, t dbSNP:371271170
831 831 a, g dbSNP:377615121
835 835 c, t dbSNP:74410123
837 837 c, t dbSNP:121918173
838 838 c, g dbSNP:764018631
839 839 c, t dbSNP:756033004
840 840 a, c dbSNP:200451572
850 850 a, g dbSNP:767451327
856 856 a, g dbSNP:759010501
860 860 a, g dbSNP:757058191
862 862 a, g dbSNP:398123235
875 875 a, g dbSNP:369190724
878 878 g, t dbSNP:149761698
885 885 a, c, t dbSNP:749520560
886 886 a, g dbSNP:776843811
888 888 c, t dbSNP:376589676
889 889 a, g dbSNP:556424939
905 905 c, t dbSNP:375214425
906 906 a, g dbSNP:200149500
908 908 c, t dbSNP:745919844
912 912 -, c dbSNP:761247245
915 915 c, t dbSNP:779091113
917 917 c, t dbSNP:757418833
918 918 a, g dbSNP:749582075
920 920 c, t dbSNP:777871501
921 921 a, g dbSNP:755842317
929 929 a, g dbSNP:747608667
931 931 c, t dbSNP:752523400
932 932 a, g dbSNP:767396235
933 933 c, t dbSNP:150686327
935 935 c, g dbSNP:367551992
937 937 c, t dbSNP:751025746
946 946 g, t dbSNP:193920810
949 949 a, g dbSNP:771376085
954 954 a, g dbSNP:763043756
960 960 c, t dbSNP:773334034
964 964 a, g dbSNP:769976774
968 968 c, t dbSNP:748423878
978 978 a, g dbSNP:376131143
981 981 a, g dbSNP:768428238
982 982 c, t dbSNP:766849255
984 984 a, g dbSNP:143040697
990 990 a, g dbSNP:369767887
993 993 -, gtg dbSNP:768543128
996 996 c, t dbSNP:758314777
997 997 a, g dbSNP:749917538
1006 1006 a, g dbSNP:778631803
1012 1012 -, c dbSNP:35957142
1016 1016 c, t dbSNP:368144710
1017 1017 a, g dbSNP:753483823
1018 1018 c, t dbSNP:763845326
1022 1022 c, t dbSNP:139776224
1028 1028 a, g dbSNP:751894676
1031 1031 a, g dbSNP:121918180
1043 1043 a, c, t dbSNP:376567545
1052 1052 a, g dbSNP:118066970
1054 1054 c, g dbSNP:769803627
1062 1062 a, g dbSNP:761881997
1076 1076 c, t dbSNP:776791543
1094 1094 c, t dbSNP:764344489
1095 1095 a, g dbSNP:752854143
1098 1098 c, t dbSNP:775341321
1108 1108 c, t dbSNP:771707097
1109 1109 c, t dbSNP:138554083
1112 1112 a, g dbSNP:745662610
1122 1122 c, t dbSNP:774393243
1123 1123 a, g dbSNP:771017036
1124 1124 a, g dbSNP:748788618
1136 1136 c, t dbSNP:777191171
1147 1147 c, t dbSNP:755791810
1154 1154 c, t dbSNP:200831987
1163 1163 c, t dbSNP:143084006
1165 1165 -, a dbSNP:771250055
1173 1173 c, g dbSNP:143495981
1174 1174 c, t dbSNP:747719992
1175 1175 a, g dbSNP:548880426
1177 1177 a, g dbSNP:121918178
1190 1190 c, g dbSNP:760449944
1204 1204 a, g dbSNP:768343264
1208 1208 c, t dbSNP:746198220
1214 1214 a, g dbSNP:121918179
1219 1219 -, a dbSNP:778161730
1220 1220 a, c, t dbSNP:201928248
1221 1221 a, g dbSNP:778349139
1225 1225 a, g dbSNP:199694121
1226 1226 c, t dbSNP:752845837
1246 1246 a, c dbSNP:767826344
1254 1254 c, g dbSNP:759880257
1275 1275 c, t dbSNP:751397640
1278 1278 c, t dbSNP:767730315
1279 1279 a, g dbSNP:786205674
1280 1280 c, t dbSNP:766339888
1284 1284 -, aag dbSNP:758492902
1298 1298 c, t dbSNP:762859080
1310 1310 c, t dbSNP:377519272
1313 1313 a, g dbSNP:368053364
1316 1316 -, t dbSNP:756256226
1316 1316 c, t dbSNP:769788461
1328 1328 a, g dbSNP:761204529
1337 1337 a, g dbSNP:776242697
1346 1346 c, g dbSNP:768292111
1347 1347 c, g dbSNP:760181741
1356 1356 c, t dbSNP:775084641
1373 1373 a, g dbSNP:771735289
1380 1380 a, c dbSNP:749594772
1387 1387 g, t dbSNP:149448152
1388 1388 c, g dbSNP:770200580
1392 1392 a, g dbSNP:748750514
1418 1418 a, c dbSNP:781768877
1420 1420 a, g dbSNP:755045141
1422 1422 c, t dbSNP:747104326
1423 1423 g, t dbSNP:121918183
1431 1431 c, t dbSNP:780397637
1433 1433 c, t dbSNP:1061361
1434 1434 a, g dbSNP:150304382
1435 1435 c, t dbSNP:765136502
1439 1439 c, t dbSNP:373819742
1440 1440 a, g dbSNP:757015172
1445 1445 a, g dbSNP:141430018
1449 1449 a, t dbSNP:764050758
1463 1463 a, c, t dbSNP:200228580
1470 1470 a, g dbSNP:113059520
1472 1472 a, c, g dbSNP:564360146
1481 1481 a, g dbSNP:759301811
1484 1484 a, g dbSNP:767115386
1486 1486 c, t dbSNP:758984878
1487 1487 a, g dbSNP:751161248
1489 1489 c, t dbSNP:765983374
1490 1490 a, g dbSNP:552724524
1495 1495 g, t dbSNP:762241590
1515 1515 a, c dbSNP:777173084
1524 1524 c, t dbSNP:121918176
1538 1538 -, a dbSNP:35302712
1538 1538 a, t dbSNP:761319717
1543 1543 g, t dbSNP:775873162
1546 1546 c, g dbSNP:772122474
1549 1549 c, t dbSNP:121918172
1550 1550 a, g dbSNP:779147312
1560 1560 c, t dbSNP:751840849
1567 1567 a, g dbSNP:372550726
1569 1569 c, t dbSNP:777613366
1574 1574 g, t dbSNP:121918184
1580 1580 g, t dbSNP:756035101
1584 1584 a, c dbSNP:752576315
1585 1585 a, g dbSNP:780999350
1591 1591 c, t dbSNP:754474825
1594 1594 c, g dbSNP:751075889
1595 1595 a, g dbSNP:766019399
1598 1598 -, t dbSNP:762840002
1599 1599 c, t dbSNP:145910162
1600 1600 a, c dbSNP:762757920
1611 1611 -, tag dbSNP:770237165
1618 1618 c, t dbSNP:754238750
1622 1622 a, g dbSNP:764493374
1634 1634 -, c dbSNP:372007777
1635 1635 a, c, g dbSNP:776024156
1638 1638 c, t dbSNP:772478693
1639 1639 c, t dbSNP:9530
1639 1639 c, t dbSNP:544927524
1640 1640 a, g dbSNP:774336516
1645 1645 c, g dbSNP:557221338
1647 1647 g, t dbSNP:771138602
1653 1653 a, t dbSNP:201204553
1661 1661 a, c, g dbSNP:769514976
1662 1662 a, c dbSNP:747928978
1667 1667 -, t dbSNP:769662185
1669 1669 c, t dbSNP:150311243
1670 1670 a, g dbSNP:754908402
1672 1672 a, g dbSNP:751073317
1674 1674 c, t dbSNP:779485917
1683 1683 c, g dbSNP:62470933
1686 1686 a, g dbSNP:757993425
1690 1690 c, g, t dbSNP:765012426
1695 1695 c, t dbSNP:756497692
1699 1699 a, t dbSNP:753051726
1704 1704 c, t dbSNP:372605666
1718 1718 c, g dbSNP:753596986
1736 1736 a, g dbSNP:567259021
1743 1743 a, g dbSNP:536903750
1797 1797 a, g dbSNP:568655640
1810 1810 a, g dbSNP:140236850
1821 1821 -, a dbSNP:762261099
1821 1821 a, g dbSNP:77293332
1843 1843 a, g dbSNP:138519677
1857 1857 g, t dbSNP:190201470

Target ORF information:

RefSeq Version NM_001284290
Organism Homo sapiens (human)
Definition Homo sapiens glucuronidase, beta (GUSB), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001284290

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCCGGG GGTCGGCGGT TGCCTGGGCG GCGCTCGGGC CGTTGTTGTG GGGCTGCGCG 
CTGGGGCTGC AGGGCGGGAT GCTGTACCCC CAGGAGAGCC CGTCGCGGGA GTGCAAGGAG
CTGGACGGCC TCTGGAGCTT CCGCGCCGAC TTCTCTGACA ACCGACGCCG GGGCTTCGAG
GAGCAGTGGT ACCGGCGGCC GCTGTGGGAG TCAGGCCCCA CCGTGGACAT GCCAGTTCCC
TCCAGCTTCA ATGACATCAG CCAGGACTGG CGTCTGCGGC ATTTTGTCGG CTGGGTGTGG
TACGAACGGG AGGTGATCCT GCCGGAGCGA TGGACCCAGG ACCTGCGCAC AAGAGTGGTG
CTGAGGATTG GCAGTGCCCA TTCCTATGCC ATCGTGTGGG TGAATGGGGT CGACACGCTA
GAGCATGAGG GGGGCTACCT CCCCTTCGAG GCCGACATCA GCAACCTGGT CCAGGTGCAG
CTGACTGCAC AGACGTCACT GGGGCCTGTG TCTGACTTCT ACACACTCCC TGTGGGGATC
CGCACTGTGG CTGTCACCAA GAGCCAGTTC CTCATCAATG GGAAACCTTT CTATTTCCAC
GGTGTCAACA AGCATGAGGA TGCGGACATC CGAGGGAAGG GCTTCGACTG GCCGCTGCTG
GTGAAGGACT TCAACCTGCT TCGCTGGCTT GGTGCCAACG CTTTCCGTAC CAGCCACTAC
CCCTATGCAG AGGAAGTGAT GCAGATGTGT GACCGCTATG GGATTGTGGT CATCGATGAG
TGTCCCGGCG TGGGCCTGGC GCTGCCGCAG TTCTTCAACA ACGTTTCTCT GCATCACCAC
ATGCAGGTGA TGGAAGAAGT GGTGCGTAGG GACAAGAACC ACCCCGCGGT CGTGATGTGG
TCTGTGGCCA ACGAGCCTGC GTCCCACCTA GAATCTGCTG GCTACTACTT GAAGATGGTG
ATCGCTCACA CCAAATCCTT GGACCCCTCC CGGCCTGTGA CCTTTGTGAG CAACTCTAAC
TATGCAGCAG ACAAGGGGGC TCCGTATGTG GATGTGATCT GTTTGAACAG CTACTACTCT
TGGTATCACG ACTACGGGCA CCTGGAGTTG ATTCAGCTGC AGCTGGCCAC CCAGTTTGAG
AACTGGTATA AGAAGTATCA GAAGCCCATT ATTCAGAGCG AGTATGGAGC AGAAACGATT
GCAGGGTTTC ACCAGGATCC ACCTCTGATG TTCACTGAAG AGTACCAGAA AAGTCTGCTA
GAGCAGTACC ATCTGGGTCT GGATCAAAAA CGCAGAAAAT ACGTGGTTGG AGAGCTCATT
TGGAATTTTG CCGATTTCAT GACTGAACAG TCACCGACGA GAGTGCTGGG GAATAAAAAG
GGGATCTTCA CTCGGCAGAG ACAACCAAAA AGTGCAGCGT TCCTTTTGCG AGAGAGATAC
TGGAAGATTG CCAATGAAAC CAGGTATCCC CACTCAGTAG CCAAGTCACA ATGTTTGGAA
AACAGCCTGT TTACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27738
Accession Version NM_001293104.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1386bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product beta-glucuronidase isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC346632.1, CD014092.1, BC014142.2 and AA947121.1. Summary: This gene encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. The enzyme forms a homotetramer that is localized to the lysosome. Mutations in this gene result in mucopolysaccharidosis type VII. Alternative splicing results in multiple transcript variants. There are many pseudogenes of this locus in the human genome.[provided by RefSeq, May 2014]. Transcript Variant: This variant (3) lacks an alternate exon in the 5' coding region, compared to variant 1. The encoded protein (isoform 3) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD014092.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCATCGT GGTATCCCAA GGGTTACTTT GTCCAGAACA CATATTTTGA CTTTTTCAAC 
TACGCTGGAC TGCAGCGGTC TGTACTTCTG TACACGACAC CCACCACCTA CATCGATGAC
ATCACCGTCA CCACCAGCGT GGAGCAAGAC AGTGGGCTGG TGAATTACCA GATCTCTGTC
AAGGGCAGTA ACCTGTTCAA GTTGGAAGTG CGTCTTTTGG ATGCAGAAAA CAAAGTCGTG
GCGAATGGGA CTGGGACCCA GGGCCAACTT AAGGTGCCAG GTGTCAGCCT CTGGTGGCCG
TACCTGATGC ACGAACGCCC TGCCTATCTG TATTCATTGG AGGTGCAGCT GACTGCACAG
ACGTCACTGG GGCCTGTGTC TGACTTCTAC ACACTCCCTG TGGGGATCCG CACTGTGGCT
GTCACCAAGA GCCAGTTCCT CATCAATGGG AAACCTTTCT ATTTCCACGG TGTCAACAAG
CATGAGGATG CGGACATCCG AGGGAAGGGC TTCGACTGGC CGCTGCTGGT GAAGGACTTC
AACCTGCTTC GCTGGCTTGG TGCCAACGCT TTCCGTACCA GCCACTACCC CTATGCAGAG
GAAGTGATGC AGATGTGTGA CCGCTATGGG ATTGTGGTCA TCGATGAGTG TCCCGGCGTG
GGCCTGGCGC TGCCGCAGTT CTTCAACAAC GTTTCTCTGC ATCACCACAT GCAGGTGATG
GAAGAAGTGG TGCGTAGGGA CAAGAACCAC CCCGCGGTCG TGATGTGGTC TGTGGCCAAC
GAGCCTGCGT CCCACCTAGA ATCTGCTGGC TACTACTTGA AGATGGTGAT CGCTCACACC
AAATCCTTGG ACCCCTCCCG GCCTGTGACC TTTGTGAGCA ACTCTAACTA TGCAGCAGAC
AAGGGGGCTC CGTATGTGGA TGTGATCTGT TTGAACAGCT ACTACTCTTG GTATCACGAC
TACGGGCACC TGGAGTTGAT TCAGCTGCAG CTGGCCACCC AGTTTGAGAA CTGGTATAAG
AAGTATCAGA AGCCCATTAT TCAGAGCGAG TATGGAGCAG AAACGATTGC AGGGTTTCAC
CAGGATCCAC CTCTGATGTT CACTGAAGAG TACCAGAAAA GTCTGCTAGA GCAGTACCAT
CTGGGTCTGG ATCAAAAACG CAGAAAATAC GTGGTTGGAG AGCTCATTTG GAATTTTGCC
GATTTCATGA CTGAACAGTC ACCGACGAGA GTGCTGGGGA ATAAAAAGGG GATCTTCACT
CGGCAGAGAC AACCAAAAAG TGCAGCGTTC CTTTTGCGAG AGAGATACTG GAAGATTGCC
AATGAAACCA GGTATCCCCA CTCAGTAGCC AAGTCACAAT GTTTGGAAAA CAGCCTGTTT
ACTTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001280033.1
CDS517..1902
Misc Feature(1)493..495(+)
Misc Feature(2)622..927(+)
Misc Feature(3)931..1839(+)
Exon (1)1..341
Gene:GUSB
Gene Synonym:
Exon (2)342..527
Gene:GUSB
Gene Synonym:
Exon (3)528..670
Gene:GUSB
Gene Synonym:
Exon (4)671..858
Gene:GUSB
Gene Synonym:
Exon (5)859..1011
Gene:GUSB
Gene Synonym:
Exon (6)1012..1190
Gene:GUSB
Gene Synonym:
Exon (7)1191..1337
Gene:GUSB
Gene Synonym:
Exon (8)1338..1422
Gene:GUSB
Gene Synonym:
Exon (9)1423..1599
Gene:GUSB
Gene Synonym:
Exon (10)1600..1735
Gene:GUSB
Gene Synonym:
Exon (11)1736..2115
Gene:GUSB
Gene Synonym:
Translation
Position Chain Variation Link
20 20 c, g dbSNP:549392182
37 37 a, g dbSNP:748697743
53 53 g, t dbSNP:528932289
60 60 g, t dbSNP:2279904
80 80 c, g dbSNP:777048370
82 82 c, t dbSNP:201504866
90 90 g, t dbSNP:199591754
99 99 c, g dbSNP:760455943
112 112 c, g, t dbSNP:577269089
113 113 c, g dbSNP:771999951
116 116 c, t dbSNP:745843396
117 117 c, g dbSNP:781149654
120 120 a, g dbSNP:2279903
122 122 a, t dbSNP:540793200
125 125 a, g dbSNP:777339764
130 130 g, t dbSNP:755795388
131 131 c, t dbSNP:747348554
136 136 c, t dbSNP:780330659
137 137 c, g dbSNP:758903707
138 138 c, t dbSNP:750955623
139 139 g, t dbSNP:765390811
140 140 g, t dbSNP:757357472
141 141 c, g dbSNP:572131903
143 143 a, g dbSNP:753976305
144 144 c, t dbSNP:190496263
145 145 c, t dbSNP:535382125
147 147 a, g dbSNP:775335168
153 153 g, t dbSNP:376505707
185 185 c, t dbSNP:759514074
188 188 c, g, t dbSNP:573989102
215 215 a, g dbSNP:748756976
217 217 a, g dbSNP:772729596
219 219 a, c, t dbSNP:747792546
221 221 c, t dbSNP:745503914
231 231 c, t dbSNP:780459919
236 236 c, g dbSNP:758743865
242 242 g, t dbSNP:746254536
243 243 c, t dbSNP:779499448
249 249 a, g dbSNP:757874872
255 255 g, t dbSNP:753922918
269 269 c, g dbSNP:764321712
278 278 c, t dbSNP:375940373
281 281 c, t dbSNP:752931066
288 288 a, g dbSNP:371314015
292 292 a, g dbSNP:557347158
293 293 c, t dbSNP:74975849
297 297 c, t dbSNP:147262894
300 300 c, g, t dbSNP:772676579
301 301 g, t dbSNP:769252159
302 302 a, g dbSNP:567066124
316 316 a, g dbSNP:571736757
327 327 c, t dbSNP:776085845
328 328 g, t dbSNP:768362767
332 332 g, t dbSNP:551892631
333 333 c, t dbSNP:746199104
347 347 c, t dbSNP:77774169
348 348 a, c dbSNP:748127836
353 353 c, g, t dbSNP:140016611
361 361 g, t dbSNP:751845855
363 363 c, g dbSNP:779807872
364 364 c, t dbSNP:758127615
380 380 c, t dbSNP:750221073
382 382 a, g dbSNP:765042077
390 390 a, g dbSNP:757243831
391 391 g, t dbSNP:753270240
392 392 c, t dbSNP:112435030
397 397 a, g dbSNP:398123236
402 402 c, t dbSNP:763704693
403 403 a, g dbSNP:147454954
408 408 c, t dbSNP:769047951
409 409 a, g dbSNP:767292825
417 417 g, t dbSNP:763344202
419 419 c, t dbSNP:773646634
420 420 a, g dbSNP:770176365
421 421 a, g dbSNP:748729882
426 426 a, g dbSNP:398123237
427 427 g, t dbSNP:776569076
434 434 c, t dbSNP:768778881
438 438 c, t dbSNP:786205673
444 444 a, g dbSNP:747101015
455 455 a, g dbSNP:546131174
462 462 g, t dbSNP:200935970
464 464 c, g dbSNP:745604255
471 471 g, t dbSNP:778819412
477 477 c, g, t dbSNP:753883162
478 478 a, g, t dbSNP:538431094
482 482 a, g dbSNP:532399422
483 483 a, g dbSNP:767239546
492 492 c, g dbSNP:759309329
496 496 a, g dbSNP:773416756
511 511 a, g dbSNP:765699404
514 514 c, t dbSNP:762212333
522 522 a, g dbSNP:777134888
525 525 a, g dbSNP:769151272
529 529 c, t dbSNP:372083359
557 557 c, t dbSNP:748981851
558 558 a, g dbSNP:781604428
559 559 c, t dbSNP:367989163
570 570 c, t dbSNP:747403896
574 574 a, t dbSNP:200457516
579 579 c, t dbSNP:140278510
583 583 a, g dbSNP:750505590
587 587 g, t dbSNP:779171218
590 590 a, t dbSNP:757545827
592 592 c, t dbSNP:121918174
603 603 g, t dbSNP:764393614
604 604 c, g dbSNP:760324067
609 609 c, g dbSNP:752571900
610 610 a, g dbSNP:767394322
611 611 c, t dbSNP:759521075
612 612 a, g dbSNP:773822519
615 615 a, t dbSNP:201178083
620 620 c, g dbSNP:773636939
624 624 c, t dbSNP:537957979
628 628 a, g dbSNP:772672861
630 630 a, c, t dbSNP:375590538
631 631 a, g dbSNP:780513114
633 633 g, t dbSNP:772673266
641 641 c, g dbSNP:138618819
642 642 c, t dbSNP:779124500
643 643 a, g dbSNP:202210104
653 653 c, g dbSNP:753959306
654 654 c, t dbSNP:777822251
655 655 a, g, t dbSNP:373800055
668 668 a, g dbSNP:200298579
684 684 c, g dbSNP:764866250
695 695 g, t dbSNP:761355885
706 706 a, c dbSNP:150086524
710 710 a, t dbSNP:140709901
712 712 c, t dbSNP:200941434
714 714 c, g dbSNP:774882132
725 725 c, t dbSNP:771320756
727 727 c, t dbSNP:768075787
728 728 a, g dbSNP:373590814
730 730 c, t dbSNP:769976411
742 742 a, g dbSNP:748229629
751 751 a, c, g dbSNP:755211573
753 753 c, t dbSNP:571704452
758 758 c, t dbSNP:369465888
759 759 a, g dbSNP:150999162
774 774 c, t dbSNP:758263587
783 783 a, g dbSNP:750430817
788 788 a, g dbSNP:765097533
794 794 c, t dbSNP:779460964
796 796 c, g dbSNP:756721679
807 807 c, t dbSNP:753430922
812 812 a, g dbSNP:398123238
816 816 a, g dbSNP:377665214
817 817 a, t dbSNP:760452541
820 820 c, g dbSNP:751945330
828 828 c, t dbSNP:766837276
831 831 a, g dbSNP:763358124
832 832 c, t dbSNP:773699561
833 833 a, g dbSNP:528899475
850 850 c, t dbSNP:761828939
851 851 a, c dbSNP:776736558
853 853 c, t dbSNP:569297611
860 860 -, tcataggt dbSNP:760367429
864 864 g, t dbSNP:375828604
878 878 c, t dbSNP:371554909
879 879 a, g dbSNP:188921177
883 883 c, t dbSNP:769871779
887 887 a, c, g dbSNP:376068646
890 890 c, t dbSNP:780604522
892 892 c, g dbSNP:754650149
893 893 c, t dbSNP:746697465
896 896 c, t dbSNP:779693774
901 901 c, t dbSNP:757549474
907 907 a, g dbSNP:375970959
909 909 a, g dbSNP:142706520
912 912 c, g dbSNP:764537946
934 934 g, t dbSNP:561880652
954 954 c, t dbSNP:752792335
957 957 c, t dbSNP:767561248
966 966 a, g dbSNP:372851133
969 969 a, g dbSNP:774381911
970 970 c, g dbSNP:766716968
971 971 a, c dbSNP:368189360
971 971 -, c dbSNP:772885108
977 977 a, g dbSNP:762798994
984 984 c, t dbSNP:773187021
987 987 c, t dbSNP:141303888
988 988 a, g dbSNP:531607427
990 990 c, t dbSNP:147820162
996 996 a, c, g dbSNP:121918182
997 997 c, t dbSNP:191153460
999 999 g, t dbSNP:767992971
1002 1002 a, g dbSNP:200412277
1007 1007 c, t dbSNP:121918175
1008 1008 a, g dbSNP:554759648
1011 1011 c, t dbSNP:758070679
1015 1015 c, t dbSNP:121918185
1029 1029 c, t dbSNP:139976112
1030 1030 a, g dbSNP:398123234
1037 1037 c, t dbSNP:771629102
1038 1038 a, g dbSNP:759063684
1042 1042 c, g dbSNP:535768789
1045 1045 a, g dbSNP:770650835
1056 1056 c, g dbSNP:374490058
1062 1062 a, g dbSNP:778654745
1066 1066 c, g, t dbSNP:747572640
1067 1067 a, g dbSNP:780716481
1072 1072 c, t dbSNP:11559283
1080 1080 c, t dbSNP:374755881
1083 1083 a, c, g, t dbSNP:371271170
1084 1084 a, g dbSNP:377615121
1088 1088 c, t dbSNP:74410123
1090 1090 c, t dbSNP:121918173
1091 1091 c, g dbSNP:764018631
1092 1092 c, t dbSNP:756033004
1093 1093 a, c dbSNP:200451572
1103 1103 a, g dbSNP:767451327
1109 1109 a, g dbSNP:759010501
1113 1113 a, g dbSNP:757058191
1115 1115 a, g dbSNP:398123235
1128 1128 a, g dbSNP:369190724
1131 1131 g, t dbSNP:149761698
1138 1138 a, c, t dbSNP:749520560
1139 1139 a, g dbSNP:776843811
1141 1141 c, t dbSNP:376589676
1142 1142 a, g dbSNP:556424939
1158 1158 c, t dbSNP:375214425
1159 1159 a, g dbSNP:200149500
1161 1161 c, t dbSNP:745919844
1165 1165 -, c dbSNP:761247245
1168 1168 c, t dbSNP:779091113
1170 1170 c, t dbSNP:757418833
1171 1171 a, g dbSNP:749582075
1173 1173 c, t dbSNP:777871501
1174 1174 a, g dbSNP:755842317
1182 1182 a, g dbSNP:747608667
1184 1184 c, t dbSNP:752523400
1185 1185 a, g dbSNP:767396235
1186 1186 c, t dbSNP:150686327
1188 1188 c, g dbSNP:367551992
1190 1190 c, t dbSNP:751025746
1199 1199 g, t dbSNP:193920810
1202 1202 a, g dbSNP:771376085
1207 1207 a, g dbSNP:763043756
1213 1213 c, t dbSNP:773334034
1217 1217 a, g dbSNP:769976774
1221 1221 c, t dbSNP:748423878
1231 1231 a, g dbSNP:376131143
1234 1234 a, g dbSNP:768428238
1235 1235 c, t dbSNP:766849255
1237 1237 a, g dbSNP:143040697
1243 1243 a, g dbSNP:369767887
1246 1246 -, gtg dbSNP:768543128
1249 1249 c, t dbSNP:758314777
1250 1250 a, g dbSNP:749917538
1259 1259 a, g dbSNP:778631803
1265 1265 -, c dbSNP:35957142
1269 1269 c, t dbSNP:368144710
1270 1270 a, g dbSNP:753483823
1271 1271 c, t dbSNP:763845326
1275 1275 c, t dbSNP:139776224
1281 1281 a, g dbSNP:751894676
1284 1284 a, g dbSNP:121918180
1296 1296 a, c, t dbSNP:376567545
1305 1305 a, g dbSNP:118066970
1307 1307 c, g dbSNP:769803627
1315 1315 a, g dbSNP:761881997
1329 1329 c, t dbSNP:776791543
1347 1347 c, t dbSNP:764344489
1348 1348 a, g dbSNP:752854143
1351 1351 c, t dbSNP:775341321
1361 1361 c, t dbSNP:771707097
1362 1362 c, t dbSNP:138554083
1365 1365 a, g dbSNP:745662610
1375 1375 c, t dbSNP:774393243
1376 1376 a, g dbSNP:771017036
1377 1377 a, g dbSNP:748788618
1389 1389 c, t dbSNP:777191171
1400 1400 c, t dbSNP:755791810
1407 1407 c, t dbSNP:200831987
1416 1416 c, t dbSNP:143084006
1418 1418 -, a dbSNP:771250055
1426 1426 c, g dbSNP:143495981
1427 1427 c, t dbSNP:747719992
1428 1428 a, g dbSNP:548880426
1430 1430 a, g dbSNP:121918178
1443 1443 c, g dbSNP:760449944
1457 1457 a, g dbSNP:768343264
1461 1461 c, t dbSNP:746198220
1467 1467 a, g dbSNP:121918179
1472 1472 -, a dbSNP:778161730
1473 1473 a, c, t dbSNP:201928248
1474 1474 a, g dbSNP:778349139
1478 1478 a, g dbSNP:199694121
1479 1479 c, t dbSNP:752845837
1499 1499 a, c dbSNP:767826344
1507 1507 c, g dbSNP:759880257
1528 1528 c, t dbSNP:751397640
1531 1531 c, t dbSNP:767730315
1532 1532 a, g dbSNP:786205674
1533 1533 c, t dbSNP:766339888
1537 1537 -, aag dbSNP:758492902
1551 1551 c, t dbSNP:762859080
1563 1563 c, t dbSNP:377519272
1566 1566 a, g dbSNP:368053364
1569 1569 -, t dbSNP:756256226
1569 1569 c, t dbSNP:769788461
1581 1581 a, g dbSNP:761204529
1590 1590 a, g dbSNP:776242697
1599 1599 c, g dbSNP:768292111
1600 1600 c, g dbSNP:760181741
1609 1609 c, t dbSNP:775084641
1626 1626 a, g dbSNP:771735289
1633 1633 a, c dbSNP:749594772
1640 1640 g, t dbSNP:149448152
1641 1641 c, g dbSNP:770200580
1645 1645 a, g dbSNP:748750514
1671 1671 a, c dbSNP:781768877
1673 1673 a, g dbSNP:755045141
1675 1675 c, t dbSNP:747104326
1676 1676 g, t dbSNP:121918183
1684 1684 c, t dbSNP:780397637
1686 1686 c, t dbSNP:1061361
1687 1687 a, g dbSNP:150304382
1688 1688 c, t dbSNP:765136502
1692 1692 c, t dbSNP:373819742
1693 1693 a, g dbSNP:757015172
1698 1698 a, g dbSNP:141430018
1702 1702 a, t dbSNP:764050758
1716 1716 a, c, t dbSNP:200228580
1723 1723 a, g dbSNP:113059520
1725 1725 a, c, g dbSNP:564360146
1734 1734 a, g dbSNP:759301811
1737 1737 a, g dbSNP:767115386
1739 1739 c, t dbSNP:758984878
1740 1740 a, g dbSNP:751161248
1742 1742 c, t dbSNP:765983374
1743 1743 a, g dbSNP:552724524
1748 1748 g, t dbSNP:762241590
1768 1768 a, c dbSNP:777173084
1777 1777 c, t dbSNP:121918176
1791 1791 -, a dbSNP:35302712
1791 1791 a, t dbSNP:761319717
1796 1796 g, t dbSNP:775873162
1799 1799 c, g dbSNP:772122474
1802 1802 c, t dbSNP:121918172
1803 1803 a, g dbSNP:779147312
1813 1813 c, t dbSNP:751840849
1820 1820 a, g dbSNP:372550726
1822 1822 c, t dbSNP:777613366
1827 1827 g, t dbSNP:121918184
1833 1833 g, t dbSNP:756035101
1837 1837 a, c dbSNP:752576315
1838 1838 a, g dbSNP:780999350
1844 1844 c, t dbSNP:754474825
1847 1847 c, g dbSNP:751075889
1848 1848 a, g dbSNP:766019399
1851 1851 -, t dbSNP:762840002
1852 1852 c, t dbSNP:145910162
1853 1853 a, c dbSNP:762757920
1864 1864 -, tag dbSNP:770237165
1871 1871 c, t dbSNP:754238750
1875 1875 a, g dbSNP:764493374
1887 1887 -, c dbSNP:372007777
1888 1888 a, c, g dbSNP:776024156
1891 1891 c, t dbSNP:772478693
1892 1892 c, t dbSNP:9530
1892 1892 c, t dbSNP:544927524
1893 1893 a, g dbSNP:774336516
1898 1898 c, g dbSNP:557221338
1900 1900 g, t dbSNP:771138602
1906 1906 a, t dbSNP:201204553
1914 1914 a, c, g dbSNP:769514976
1915 1915 a, c dbSNP:747928978
1920 1920 -, t dbSNP:769662185
1922 1922 c, t dbSNP:150311243
1923 1923 a, g dbSNP:754908402
1925 1925 a, g dbSNP:751073317
1927 1927 c, t dbSNP:779485917
1936 1936 c, g dbSNP:62470933
1939 1939 a, g dbSNP:757993425
1943 1943 c, g, t dbSNP:765012426
1948 1948 c, t dbSNP:756497692
1952 1952 a, t dbSNP:753051726
1957 1957 c, t dbSNP:372605666
1971 1971 c, g dbSNP:753596986
1989 1989 a, g dbSNP:567259021
1996 1996 a, g dbSNP:536903750
2050 2050 a, g dbSNP:568655640
2063 2063 a, g dbSNP:140236850
2074 2074 -, a dbSNP:762261099
2074 2074 a, g dbSNP:77293332
2096 2096 a, g dbSNP:138519677
2110 2110 g, t dbSNP:190201470

Target ORF information:

RefSeq Version NM_001293104
Organism Homo sapiens (human)
Definition Homo sapiens glucuronidase, beta (GUSB), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001293104

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCATCGT GGTATCCCAA GGGTTACTTT GTCCAGAACA CATATTTTGA CTTTTTCAAC 
TACGCTGGAC TGCAGCGGTC TGTACTTCTG TACACGACAC CCACCACCTA CATCGATGAC
ATCACCGTCA CCACCAGCGT GGAGCAAGAC AGTGGGCTGG TGAATTACCA GATCTCTGTC
AAGGGCAGTA ACCTGTTCAA GTTGGAAGTG CGTCTTTTGG ATGCAGAAAA CAAAGTCGTG
GCGAATGGGA CTGGGACCCA GGGCCAACTT AAGGTGCCAG GTGTCAGCCT CTGGTGGCCG
TACCTGATGC ACGAACGCCC TGCCTATCTG TATTCATTGG AGGTGCAGCT GACTGCACAG
ACGTCACTGG GGCCTGTGTC TGACTTCTAC ACACTCCCTG TGGGGATCCG CACTGTGGCT
GTCACCAAGA GCCAGTTCCT CATCAATGGG AAACCTTTCT ATTTCCACGG TGTCAACAAG
CATGAGGATG CGGACATCCG AGGGAAGGGC TTCGACTGGC CGCTGCTGGT GAAGGACTTC
AACCTGCTTC GCTGGCTTGG TGCCAACGCT TTCCGTACCA GCCACTACCC CTATGCAGAG
GAAGTGATGC AGATGTGTGA CCGCTATGGG ATTGTGGTCA TCGATGAGTG TCCCGGCGTG
GGCCTGGCGC TGCCGCAGTT CTTCAACAAC GTTTCTCTGC ATCACCACAT GCAGGTGATG
GAAGAAGTGG TGCGTAGGGA CAAGAACCAC CCCGCGGTCG TGATGTGGTC TGTGGCCAAC
GAGCCTGCGT CCCACCTAGA ATCTGCTGGC TACTACTTGA AGATGGTGAT CGCTCACACC
AAATCCTTGG ACCCCTCCCG GCCTGTGACC TTTGTGAGCA ACTCTAACTA TGCAGCAGAC
AAGGGGGCTC CGTATGTGGA TGTGATCTGT TTGAACAGCT ACTACTCTTG GTATCACGAC
TACGGGCACC TGGAGTTGAT TCAGCTGCAG CTGGCCACCC AGTTTGAGAA CTGGTATAAG
AAGTATCAGA AGCCCATTAT TCAGAGCGAG TATGGAGCAG AAACGATTGC AGGGTTTCAC
CAGGATCCAC CTCTGATGTT CACTGAAGAG TACCAGAAAA GTCTGCTAGA GCAGTACCAT
CTGGGTCTGG ATCAAAAACG CAGAAAATAC GTGGTTGGAG AGCTCATTTG GAATTTTGCC
GATTTCATGA CTGAACAGTC ACCGACGAGA GTGCTGGGGA ATAAAAAGGG GATCTTCACT
CGGCAGAGAC AACCAAAAAG TGCAGCGTTC CTTTTGCGAG AGAGATACTG GAAGATTGCC
AATGAAACCA GGTATCCCCA CTCAGTAGCC AAGTCACAAT GTTTGGAAAA CAGCCTGTTT
ACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27739
Accession Version NM_001293105.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1299bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product beta-glucuronidase isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC346632.1, CD014093.1, BC014142.2 and AA947121.1. Summary: This gene encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. The enzyme forms a homotetramer that is localized to the lysosome. Mutations in this gene result in mucopolysaccharidosis type VII. Alternative splicing results in multiple transcript variants. There are many pseudogenes of this locus in the human genome.[provided by RefSeq, May 2014]. Transcript Variant: This variant (4) lacks alternate exons in the 5' coding region, compared to variant 1. The encoded protein (isoform 4) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD014093.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGACCCAG GACCTGCGCA CAAGAGTGGT GCTGAGGATT GGCAGTGCCC ATTCCTATGC 
CATCGTGGGC TGGTGAATTA CCAGATCTCT GTCAAGGGCA GTAACCTGTT CAAGTTGGAA
GTGCGTCTTT TGGATGCAGA AAACAAAGTC GTGGCGAATG GGACTGGGAC CCAGGGCCAA
CTTAAGGTGC CAGGTGTCAG CCTCTGGTGG CCGTACCTGA TGCACGAACG CCCTGCCTAT
CTGTATTCAT TGGAGGTGCA GCTGACTGCA CAGACGTCAC TGGGGCCTGT GTCTGACTTC
TACACACTCC CTGTGGGGAT CCGCACTGTG GCTGTCACCA AGAGCCAGTT CCTCATCAAT
GGGAAACCTT TCTATTTCCA CGGTGTCAAC AAGCATGAGG ATGCGGACAT CCGAGGGAAG
GGCTTCGACT GGCCGCTGCT GGTGAAGGAC TTCAACCTGC TTCGCTGGCT TGGTGCCAAC
GCTTTCCGTA CCAGCCACTA CCCCTATGCA GAGGAAGTGA TGCAGATGTG TGACCGCTAT
GGGATTGTGG TCATCGATGA GTGTCCCGGC GTGGGCCTGG CGCTGCCGCA GTTCTTCAAC
AACGTTTCTC TGCATCACCA CATGCAGGTG ATGGAAGAAG TGGTGCGTAG GGACAAGAAC
CACCCCGCGG TCGTGATGTG GTCTGTGGCC AACGAGCCTG CGTCCCACCT AGAATCTGCT
GGCTACTACT TGAAGATGGT GATCGCTCAC ACCAAATCCT TGGACCCCTC CCGGCCTGTG
ACCTTTGTGA GCAACTCTAA CTATGCAGCA GACAAGGGGG CTCCGTATGT GGATGTGATC
TGTTTGAACA GCTACTACTC TTGGTATCAC GACTACGGGC ACCTGGAGTT GATTCAGCTG
CAGCTGGCCA CCCAGTTTGA GAACTGGTAT AAGAAGTATC AGAAGCCCAT TATTCAGAGC
GAGTATGGAG CAGAAACGAT TGCAGGGTTT CACCAGGATC CACCTCTGAT GTTCACTGAA
GAGTACCAGA AAAGTCTGCT AGAGCAGTAC CATCTGGGTC TGGATCAAAA ACGCAGAAAA
TACGTGGTTG GAGAGCTCAT TTGGAATTTT GCCGATTTCA TGACTGAACA GTCACCGACG
AGAGTGCTGG GGAATAAAAA GGGGATCTTC ACTCGGCAGA GACAACCAAA AAGTGCAGCG
TTCCTTTTGC GAGAGAGATA CTGGAAGATT GCCAATGAAA CCAGGTATCC CCACTCAGTA
GCCAAGTCAC AATGTTTGGA AAACAGCCTG TTTACTTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001280034.1
CDS461..1759
Misc Feature(1)383..385(+)
Misc Feature(2)788..1696(+)
Exon (1)1..341
Gene:GUSB
Gene Synonym:
Exon (2)342..527
Gene:GUSB
Gene Synonym:
Exon (3)528..715
Gene:GUSB
Gene Synonym:
Exon (4)716..868
Gene:GUSB
Gene Synonym:
Exon (5)869..1047
Gene:GUSB
Gene Synonym:
Exon (6)1048..1194
Gene:GUSB
Gene Synonym:
Exon (7)1195..1279
Gene:GUSB
Gene Synonym:
Exon (8)1280..1456
Gene:GUSB
Gene Synonym:
Exon (9)1457..1592
Gene:GUSB
Gene Synonym:
Exon (10)1593..1972
Gene:GUSB
Gene Synonym:
Translation
Position Chain Variation Link
20 20 c, g dbSNP:549392182
37 37 a, g dbSNP:748697743
53 53 g, t dbSNP:528932289
60 60 g, t dbSNP:2279904
80 80 c, g dbSNP:777048370
82 82 c, t dbSNP:201504866
90 90 g, t dbSNP:199591754
99 99 c, g dbSNP:760455943
112 112 c, g, t dbSNP:577269089
113 113 c, g dbSNP:771999951
116 116 c, t dbSNP:745843396
117 117 c, g dbSNP:781149654
120 120 a, g dbSNP:2279903
122 122 a, t dbSNP:540793200
125 125 a, g dbSNP:777339764
130 130 g, t