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HBB cDNA ORF clone, Homo sapiens (human)

Gene Symbol HBB
Entrez Gene ID 3043
Full Name hemoglobin, beta
Synonyms CD113t-C, beta-globin
General protein information
Preferred Names
hemoglobin subunit beta
Names
hemoglobin subunit beta
beta globin chain
hemoglobin beta chain
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11p15.5

Summary The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

141900

Disorder Html: Sickle cell anemia, 603903 (3); Thalassemias, beta-, 604131 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_000518 NP_000509 hemoglobin subunit beta



Homo sapiens (human) HBB NP_000509.1
Pan troglodytes (chimpanzee) HBB XP_508242.1
Macaca mulatta (Rhesus monkey) HBB NP_001157900.1
Canis lupus familiaris (dog) LOC609402 NP_001257812.1
Canis lupus familiaris (dog) LOC476825 XP_534029.2
Canis lupus familiaris (dog) LOC480784 NP_001257813.1
Mus musculus (house mouse) Hbb-bs NP_001188320.1
Mus musculus (house mouse) Hbb-bt NP_032246.2
Rattus norvegicus (Norway rat) LOC100134871 NP_001106694.1
Rattus norvegicus (Norway rat) LOC689064 NP_001104739.1
Rattus norvegicus (Norway rat) Hbb NP_150237.1
Rattus norvegicus (Norway rat) Hbb-b1 NP_942071.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HBB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HBB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu25936
NM_000518 Homo sapiens hemoglobin, beta (HBB), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25936
Clone ID Related Accession (Same CDS sequence) NM_000518
Accession Version NM_000518.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 444bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-JUN-2015
Organism Homo sapiens (human)
Product hemoglobin subunit beta
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L48217.1. This sequence is a reference standard in the RefSeqGene project. On Feb 11, 2003 this sequence version replaced gi:13788565. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: V00497.1, BU659180.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
ATGGTGCATC TGACTCCTGA GGAGAAGTCT GCCGTTACTG CCCTGTGGGG CAAGGTGAAC 
GTGGATGAAG TTGGTGGTGA GGCCCTGGGC AGGCTGCTGG TGGTCTACCC TTGGACCCAG
AGGTTCTTTG AGTCCTTTGG GGATCTGTCC ACTCCTGATG CTGTTATGGG CAACCCTAAG
GTGAAGGCTC ATGGCAAGAA AGTGCTCGGT GCCTTTAGTG ATGGCCTGGC TCACCTGGAC
AACCTCAAGG GCACCTTTGC CACACTGAGT GAGCTGCACT GTGACAAGCT GCACGTGGAT
CCTGAGAACT TCAGGCTCCT GGGCAACGTG CTGGTCTGTG TGCTGGCCCA TCACTTTGGC
AAAGAATTCA CCCCACCAGT GCAGGCTGCC TATCAGAAAG TGGTGGCTGG TGTGGCTAAT
GCCCTGGCCC ACAAGTATCA CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000509.1
CDS51..494
Misc Feature(1)30..32(+)
Misc Feature(2)54..56(+)
Misc Feature(3)54..56(+)
Misc Feature(4)54..56(+)
Misc Feature(5)69..488(+)
Misc Feature(6)75..77(+)
Misc Feature(7)78..80(+)
Misc Feature(8)87..89(+)
Misc Feature(9)141..446(+)
Misc Feature(10)144..476(+)
Misc Feature(11)183..185(+)
Misc Feature(12)201..203(+)
Misc Feature(13)228..230(+)
Misc Feature(14)228..230(+)
Misc Feature(15)249..251(+)
Misc Feature(16)297..299(+)
Misc Feature(17)297..299(+)
Misc Feature(18)312..314(+)
Misc Feature(19)330..332(+)
Misc Feature(20)330..332(+)
Misc Feature(21)330..332(+)
Misc Feature(22)336..338(+)
Misc Feature(23)411..413(+)
Misc Feature(24)441..443(+)
Misc Feature(25)483..485(+)
Exon (1)1..142
Gene:HBB
Gene Synonym:
Exon (2)143..365
Gene:HBB
Gene Synonym:
Exon (3)366..626
Gene:HBB
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000518
Organism Homo sapiens (human)
Definition Homo sapiens hemoglobin, beta (HBB), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000518

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGTGCATC TGACTCCTGA GGAGAAGTCT GCCGTTACTG CCCTGTGGGG CAAGGTGAAC 
GTGGATGAAG TTGGTGGTGA GGCCCTGGGC AGGCTGCTGG TGGTCTACCC TTGGACCCAG
AGGTTCTTTG AGTCCTTTGG GGATCTGTCC ACTCCTGATG CTGTTATGGG CAACCCTAAG
GTGAAGGCTC ATGGCAAGAA AGTGCTCGGT GCCTTTAGTG ATGGCCTGGC TCACCTGGAC
AACCTCAAGG GCACCTTTGC CACACTGAGT GAGCTGCACT GTGACAAGCT GCACGTGGAT
CCTGAGAACT TCAGGCTCCT GGGCAACGTG CTGGTCTGTG TGCTGGCCCA TCACTTTGGC
AAAGAATTCA CCCCACCAGT GCAGGCTGCC TATCAGAAAG TGGTGGCTGG TGTGGCTAAT
GCCCTGGCCC ACAAGTATCA CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

[Intron 2 of human beta-globin in 3'-untranslated region enhances expression of chimeric genes]
Bioorg. Khim. 40 (3), 293-296 (2014)
Pereverzev,A.P., Markina,N.M., Ianushevich,Iu.G., Gorodnicheva,T.V., Minasian,B.E., Luk'ianov,K.A. and Gurskaia,N.G.


book

Blood typing profile of a school-aged population of a North Togo township
Hemoglobin 38 (5), 316-318 (2014)
Vovor A, Feteke L, Kueviakoe IM, Kpatarou L, Mawussi K, Magnang H and Segbena AY.


book

The correlation of alpha-globin gene mutations and the XmnI polymorphism with clinical severity of Hb E/beta-thalassemia
Hemoglobin 38 (5), 335-338 (2014)
Charoenkwan P, Teerachaimahit P and Sanguansermsri T.


book

beta-Thalassemia mutations in Western India: outcome of prenatal diagnosis in a hemoglobinopathies project
Hemoglobin 38 (5), 329-334 (2014)
Patel AP, Patel RB, Patel SA, Vaniawala SN, Patel DS, Shrivastava NS, Sharma NP, Zala JV, Parmar PH and Naik MR.


book

Upstream open reading frames cause widespread reduction of protein expression and are polymorphic among humans
Proc. Natl. Acad. Sci. U.S.A. 106 (18), 7507-7512 (2009)
Calvo SE, Pagliarini DJ and Mootha VK.


book

Beta-Thalassemia
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Origa,R.


book

Sickle Cell Disease
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Bender,M.A. and Douthitt Seibel,G.


book

The structure of hemoglobin Indianapolis [beta112(G14) arginine]. An unstable variant detectable only by isotopic labeling
J. Biol. Chem. 254 (9), 3479-3482 (1979)
Adams,J.G., Steinberg,M.H., Boxer,L.A., Baehner,R.L., Forget,B.G. and Tsistrakis,G.A.


book

Human beta-globin messenger RNA. III. Nucleotide sequences derived from complementary DNA
J. Biol. Chem. 252 (14), 5040-5053 (1977)
Marotta,C.A., Wilson,J.T., Forget,B.G. and Weissman,S.M.


book

Complete 3' noncoding region sequences of rabbit and human beta-globin messenger RNAs
Cell 10 (4), 559-570 (1977)
Proudfoot,N.J.


 
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