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HGD homogentisate 1,2-dioxygenase [Homo sapiens (human)]

Gene Symbol HGD
Entrez Gene ID 3081
Full Name homogentisate 1,2-dioxygenase
Synonyms AKU, HGO
General protein information
Preferred Names
homogentisate 1,2-dioxygenase
Names
homogentisate 1,2-dioxygenase
homogentisicase
homogentisate oxidase
homogentisate oxygenase
homogentisic acid oxidase
NP_000178.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

3

3q13.33

Summary This gene encodes the enzyme homogentisate 1,2 dioxygenase. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria.[provided by RefSeq, May 2010]. lac of sum
Disorder MIM:

607474

Disorder Html: Alkaptonuria, 203500 (3)





The following HGD gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HGD gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu37912 XM_005247412 PREDICTED: Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu37913 XM_005247413 PREDICTED: Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu58205 XM_011512746 PREDICTED: Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu37914 XM_005247414 PREDICTED: Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu20740 NM_000187 Homo sapiens homogentisate 1,2-dioxygenase (HGD), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $379

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu37912
Accession Version XM_005247412.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1113bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product homogentisate 1,2-dioxygenase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005612.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGG ATGTCTCCCC GTTCAATGTT GTGGCCTGGC ACGGGAATTA TACACCCTAC
AAGTACAACC TGAAGAATTT CATGGTTATC AACTCAGTGG CCTTTGACCA TGCAGACCCA
TCCATTTTCA CAGTATTGAC TGCTAAGTCT GTCCGCCCTG GAGTGGCCAT TGCTGATTTT
GTCATCTTCC CACCTCGATG GGGGGTTGCT GATAAGACCT TCAGGCCTCC TTATTACCAT
AGGAACTGCA TGAGTGAGTT CATGGGACTC ATCCGAGGTC ACTATGAGGC AAAGCAAGGT
GGGTTCCTGC CAGGGGGAGG GAGTCTACAC AGCACAATGA CCCCCCATGG ACCTGATGCT
GACTGCTTTG AGAAGGCCAG CAAGGTCAAG CTGGCACCTG AGAGGATTGC CGATGGCACC
ATGGCATTTA TGTTTGAATC ATCTTTAAGT CTGGCGGTCA CAAAGTGGGG ACTCAAGGCC
TCCAGGTGTT TGGATGAGAA CTACCACAAG TGCTGGGAGC CACTCAAGAG CCACTTCACT
CCCAACTCCA GGAACCCAGC AGAACCTAAT TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005247469.1
CDS461..1573
Misc Feature(1)467..1537(+)
Translation
Position Chain Variation Link
9 9 g, t dbSNP:142298361
11 11 a, c dbSNP:2733824
15 15 g, t dbSNP:541656880
78 78 c, g dbSNP:148732655
94 94 c, g dbSNP:555576732
122 122 c, t dbSNP:2733829
164 164 a, g dbSNP:761807054
240 240 a, g dbSNP:535973482
243 243 a, g dbSNP:553200191
254 254 a, g dbSNP:147272996
260 260 a, g dbSNP:55661952
280 280 -, c dbSNP:397875516
298 298 c, t dbSNP:556156265
322 322 a, t dbSNP:539301020
324 324 a, g dbSNP:570423502
331 331 c, g dbSNP:760347090
335 335 g, t dbSNP:547327873
341 341 a, g dbSNP:191643714
353 353 c, t dbSNP:568270873
361 361 a, g dbSNP:547960368
405 405 g, t dbSNP:371878850
412 412 c, t dbSNP:747957946
419 419 a, g dbSNP:778619933
421 421 c, t dbSNP:768585000
435 435 a, g dbSNP:531449155
442 442 c, t dbSNP:376849236
443 443 a, g dbSNP:755624842
444 444 c, t dbSNP:749915521
448 448 c, g dbSNP:775087041
450 450 a, g dbSNP:371972791
457 457 a, g dbSNP:368515284
468 468 a, c dbSNP:200412910
471 471 a, t dbSNP:786204422
478 478 c, t dbSNP:769728875
484 484 c, t dbSNP:745386219
487 487 a, t dbSNP:780784207
492 492 a, g dbSNP:370453859
499 499 a, g dbSNP:751317891
500 500 c, t dbSNP:777547119
509 509 a, g dbSNP:758729926
513 513 a, g dbSNP:753068271
514 514 a, t dbSNP:765687351
518 518 c, t dbSNP:755454185
519 519 a, g dbSNP:753865195
530 530 c, t dbSNP:766513107
540 540 a, c dbSNP:760729411
545 545 c, g dbSNP:773469019
547 547 c, g dbSNP:767692484
557 557 c, g dbSNP:775575402
560 560 a, g dbSNP:770953681
570 570 a, t dbSNP:530032005
571 571 c, t dbSNP:772792095
576 576 a, t dbSNP:771625523
579 579 a, g dbSNP:747899770
580 580 c, t dbSNP:369064890
584 584 g, t dbSNP:769112916
585 585 a, c dbSNP:373921680
589 589 a, g dbSNP:142789485
597 597 a, g dbSNP:756560798
598 598 a, g, t dbSNP:374495094
600 600 a, c, t dbSNP:369517993
601 601 a, g dbSNP:541268108
602 602 g, t dbSNP:138846036
617 617 c, t dbSNP:759435977
618 618 a, g dbSNP:200808744
621 621 a, g dbSNP:376541815
627 627 a, g dbSNP:760621801
628 628 c, t dbSNP:773277281
631 631 a, g dbSNP:771669017
634 634 -, a dbSNP:397515517
635 635 -, a dbSNP:587776556
638 638 c, t dbSNP:775113388
659 659 c, t dbSNP:370003137
681 681 a, t dbSNP:141965690
684 684 a, c dbSNP:777230877
686 686 a, g dbSNP:771294247
689 689 g, t dbSNP:747126144
691 691 c, t dbSNP:147017317
692 692 a, c, g dbSNP:748437340
695 695 a, g dbSNP:779254873
697 697 c, t dbSNP:756012521
700 700 a, t dbSNP:2255543
703 703 a, c dbSNP:377043265
712 712 c, g dbSNP:757080944
720 720 a, c dbSNP:35702995
723 723 g, t dbSNP:770398071
748 748 a, g dbSNP:776344570
750 750 a, g dbSNP:767089050
751 751 a, g dbSNP:766714128
762 762 a, t dbSNP:143267384
767 767 a, c dbSNP:559502955
773 773 a, g dbSNP:772663207
774 774 c, t dbSNP:762006383
787 787 a, g dbSNP:774514092
800 800 a, c dbSNP:201753011
801 801 c, g dbSNP:368344978
803 803 c, g dbSNP:755734596
807 807 c, t dbSNP:569846003
809 809 a, c dbSNP:768059703
819 819 a, g, t dbSNP:752153829
820 820 c, g, t dbSNP:149165166
825 825 c, t dbSNP:544956641
827 827 a, g dbSNP:564979861
828 828 a, c, g dbSNP:374473331
832 832 c, t dbSNP:140977117
833 833 a, g dbSNP:759824026
838 838 c, g dbSNP:776824088
846 846 a, g dbSNP:771906714
847 847 a, t dbSNP:747904200
850 850 g, t dbSNP:774183442
856 856 a, t dbSNP:768585616
857 857 a, g dbSNP:748757618
869 869 c, g dbSNP:779549148
885 885 c, t dbSNP:755680655
906 906 a, g dbSNP:768481821
909 909 c, t dbSNP:541367417
910 910 a, c, g dbSNP:777846799
917 917 -, g dbSNP:397515346
918 918 a, g dbSNP:775274569
927 927 c, t dbSNP:769475625
933 933 c, t dbSNP:375396766
934 934 a, g, t dbSNP:2293734
941 941 a, g dbSNP:28941783
947 947 c, t dbSNP:140543217
955 955 c, t dbSNP:754865203
957 957 a, g dbSNP:556610453
961 961 c, g, t dbSNP:146878140
962 962 a, g dbSNP:375283568
964 964 c, g dbSNP:780173554
967 967 g, t dbSNP:756134838
969 969 -, g dbSNP:755342871
985 985 a, g dbSNP:536732498
990 990 a, g dbSNP:372293676
991 991 a, t dbSNP:762831007
1001 1001 a, g dbSNP:368256121
1003 1003 c, t dbSNP:765270269
1009 1009 g, t dbSNP:759615683
1020 1020 a, c dbSNP:757937852
1021 1021 a, g dbSNP:747648606
1028 1028 a, g dbSNP:778448610
1029 1029 c, t dbSNP:554816026
1033 1033 a, g dbSNP:753425386
1040 1040 c, t dbSNP:766904253
1041 1041 a, g dbSNP:756522409
1042 1042 c, t dbSNP:758567130
1043 1043 a, g dbSNP:120074174
1057 1057 c, t dbSNP:761819710
1059 1059 a, g dbSNP:774431689
1060 1060 c, t dbSNP:764226435
1064 1064 c, t dbSNP:763294945
1067 1067 a, g dbSNP:370920635
1070 1070 a, c, t dbSNP:200382812
1072 1072 a, g dbSNP:773158062
1075 1075 a, g dbSNP:771989878
1082 1082 a, g dbSNP:747620917
1093 1093 c, g dbSNP:537736274
1108 1108 a, c dbSNP:754428438
1113 1113 a, c dbSNP:148641817
1130 1130 a, g dbSNP:372420052
1134 1134 g, t dbSNP:120074170
1140 1140 a, c dbSNP:752148612
1150 1150 c, t dbSNP:144158342
1154 1154 c, t dbSNP:143556739
1155 1155 a, g dbSNP:752719362
1157 1157 c, t dbSNP:765452552
1158 1158 c, g dbSNP:201529624
1179 1179 a, t dbSNP:754050823
1181 1181 g, t dbSNP:199927284
1189 1189 c, t dbSNP:761478895
1197 1197 a, g dbSNP:774374625
1198 1198 a, g dbSNP:768426419
1199 1199 -, g dbSNP:34214309
1201 1201 a, g dbSNP:745825659
1202 1202 a, g dbSNP:762564483
1204 1204 a, g dbSNP:376724242
1205 1205 -, g dbSNP:766354352
1208 1208 a, g dbSNP:769424424
1211 1211 a, g dbSNP:143396290
1214 1214 a, g dbSNP:372136565
1225 1225 g, t dbSNP:120074171
1227 1227 c, t dbSNP:771360138
1228 1228 c, t dbSNP:747288180
1239 1239 a, g dbSNP:778359542
1243 1243 -, g dbSNP:35952153
1249 1249 c, t dbSNP:748707886
1252 1252 g, t dbSNP:139501220
1254 1254 -, g dbSNP:762999434
1262 1262 a, c dbSNP:755078457
1264 1264 a, g dbSNP:778231396
1281 1281 a, g dbSNP:749274871
1295 1295 c, t dbSNP:780333531
1299 1299 a, g dbSNP:756222568
1304 1304 a, t dbSNP:750651924
1311 1311 c, t dbSNP:764037565
1313 1313 c, g dbSNP:368717991
1315 1315 a, g dbSNP:752713602
1316 1316 c, g dbSNP:765219004
1332 1332 a, g dbSNP:759101002
1337 1337 a, g dbSNP:120074173
1341 1341 a, c dbSNP:765912447
1345 1345 a, c dbSNP:760307527
1346 1346 -, c dbSNP:397515516
1347 1347 a, g dbSNP:120074172
1353 1353 c, t dbSNP:138558042
1363 1363 c, g dbSNP:150145204
1370 1370 c, g dbSNP:141753513
1387 1387 c, t dbSNP:774858137
1389 1389 a, c dbSNP:769091766
1392 1392 -, tggc dbSNP:772802378
1392 1392 c, t dbSNP:371538956
1397 1397 c, t dbSNP:780063156
1399 1399 c, t dbSNP:756204156
1407 1407 c, t dbSNP:746125474
1411 1411 c, t dbSNP:368805723
1412 1412 a, g dbSNP:758291793
1414 1414 c, t dbSNP:146482816
1419 1419 c, t dbSNP:765272112
1421 1421 a, g dbSNP:754940101
1423 1423 a, g dbSNP:753803362
1424 1424 a, g dbSNP:373918530
1426 1426 a, c, g dbSNP:137923025
1436 1436 c, g dbSNP:767159114
1441 1441 a, g dbSNP:140500816
1442 1442 c, t dbSNP:751197824
1455 1455 c, t dbSNP:764414572
1456 1456 a, g dbSNP:146206905
1463 1463 a, g dbSNP:775883622
1465 1465 g, t dbSNP:770278846
1472 1472 c, t dbSNP:370401523
1478 1478 a, g dbSNP:776919863
1491 1491 g, t dbSNP:771123717
1491 1491 -, t dbSNP:747809657
1498 1498 c, g dbSNP:747286602
1508 1508 a, c dbSNP:765596920
1512 1512 g, t dbSNP:768371363
1520 1520 c, g dbSNP:749059002
1524 1524 a, t dbSNP:779866537
1531 1531 c, t dbSNP:756009394
1540 1540 c, t dbSNP:571129573
1546 1546 a, c, t dbSNP:756737699
1549 1549 c, t dbSNP:751252758
1570 1570 c, t dbSNP:187614203
1571 1571 c, t dbSNP:143370662
1576 1576 -, c dbSNP:776190831
1580 1580 a, g dbSNP:753039865
1582 1582 a, c, t dbSNP:760180599
1590 1590 c, t dbSNP:777259020
1605 1605 a, g dbSNP:376051958
1615 1615 a, g dbSNP:374327803
1620 1620 a, c dbSNP:773324881
1637 1637 g, t dbSNP:762250068
1641 1641 a, g dbSNP:527905808
1695 1695 a, g dbSNP:562005702
1719 1719 a, g dbSNP:542446346
1725 1725 a, g dbSNP:542329785
1728 1728 a, g dbSNP:776810530
1732 1732 g, t dbSNP:531872369
1783 1783 a, g dbSNP:768955353

Target ORF information:

RefSeq Version XM_005247412
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005247412

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGG ATGTCTCCCC GTTCAATGTT GTGGCCTGGC ACGGGAATTA TACACCCTAC
AAGTACAACC TGAAGAATTT CATGGTTATC AACTCAGTGG CCTTTGACCA TGCAGACCCA
TCCATTTTCA CAGTATTGAC TGCTAAGTCT GTCCGCCCTG GAGTGGCCAT TGCTGATTTT
GTCATCTTCC CACCTCGATG GGGGGTTGCT GATAAGACCT TCAGGCCTCC TTATTACCAT
AGGAACTGCA TGAGTGAGTT CATGGGACTC ATCCGAGGTC ACTATGAGGC AAAGCAAGGT
GGGTTCCTGC CAGGGGGAGG GAGTCTACAC AGCACAATGA CCCCCCATGG ACCTGATGCT
GACTGCTTTG AGAAGGCCAG CAAGGTCAAG CTGGCACCTG AGAGGATTGC CGATGGCACC
ATGGCATTTA TGTTTGAATC ATCTTTAAGT CTGGCGGTCA CAAAGTGGGG ACTCAAGGCC
TCCAGGTGTT TGGATGAGAA CTACCACAAG TGCTGGGAGC CACTCAAGAG CCACTTCACT
CCCAACTCCA GGAACCCAGC AGAACCTAAT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37913
Accession Version XM_005247413.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1017bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product homogentisate 1,2-dioxygenase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005612.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGG GGCCAATGGC
TTGGCCAATC CTCGTGATTT CTTGATACCC ATTGCCTGGT ATGAGGATCG CCAAGTACCA
GGTGGTTACA CGGTCATTAA TAAATACCAG GGCAAGCTGT TTGCTGCCAA ACAGGATGTC
TCCCCGTTCA ATGTTGTGGC CTGGCACGGG AATTATACAC CCTACAAGTA CAACCTGAAG
AATTTCATGG TTATCAACTC AGTGGCCTTT GACCATGCAG ACCCATCCAT TTTCACAGTA
TTGACTGCTA AGTCTGTCCG CCCTGGAGTG GCCATTGCTG ATTTTGTCAT CTTCCCACCT
CGATGGGGGG TTGCTGATAA GACCTTCAGG CCTCCTTATT ACCATAATCT AGCCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005247470.1
CDS461..1477
Misc Feature(1)467..>1465(+)
Translation
Position Chain Variation Link
9 9 g, t dbSNP:142298361
11 11 a, c dbSNP:2733824
15 15 g, t dbSNP:541656880
78 78 c, g dbSNP:148732655
94 94 c, g dbSNP:555576732
122 122 c, t dbSNP:2733829
164 164 a, g dbSNP:761807054
240 240 a, g dbSNP:535973482
243 243 a, g dbSNP:553200191
254 254 a, g dbSNP:147272996
260 260 a, g dbSNP:55661952
280 280 -, c dbSNP:397875516
298 298 c, t dbSNP:556156265
322 322 a, t dbSNP:539301020
324 324 a, g dbSNP:570423502
331 331 c, g dbSNP:760347090
335 335 g, t dbSNP:547327873
341 341 a, g dbSNP:191643714
353 353 c, t dbSNP:568270873
361 361 a, g dbSNP:547960368
405 405 g, t dbSNP:371878850
412 412 c, t dbSNP:747957946
419 419 a, g dbSNP:778619933
421 421 c, t dbSNP:768585000
435 435 a, g dbSNP:531449155
442 442 c, t dbSNP:376849236
443 443 a, g dbSNP:755624842
444 444 c, t dbSNP:749915521
448 448 c, g dbSNP:775087041
450 450 a, g dbSNP:371972791
457 457 a, g dbSNP:368515284
468 468 a, c dbSNP:200412910
471 471 a, t dbSNP:786204422
478 478 c, t dbSNP:769728875
484 484 c, t dbSNP:745386219
487 487 a, t dbSNP:780784207
492 492 a, g dbSNP:370453859
499 499 a, g dbSNP:751317891
500 500 c, t dbSNP:777547119
509 509 a, g dbSNP:758729926
513 513 a, g dbSNP:753068271
514 514 a, t dbSNP:765687351
518 518 c, t dbSNP:755454185
519 519 a, g dbSNP:753865195
530 530 c, t dbSNP:766513107
540 540 a, c dbSNP:760729411
545 545 c, g dbSNP:773469019
547 547 c, g dbSNP:767692484
557 557 c, g dbSNP:775575402
560 560 a, g dbSNP:770953681
570 570 a, t dbSNP:530032005
571 571 c, t dbSNP:772792095
576 576 a, t dbSNP:771625523
579 579 a, g dbSNP:747899770
580 580 c, t dbSNP:369064890
584 584 g, t dbSNP:769112916
585 585 a, c dbSNP:373921680
589 589 a, g dbSNP:142789485
597 597 a, g dbSNP:756560798
598 598 a, g, t dbSNP:374495094
600 600 a, c, t dbSNP:369517993
601 601 a, g dbSNP:541268108
602 602 g, t dbSNP:138846036
617 617 c, t dbSNP:759435977
618 618 a, g dbSNP:200808744
621 621 a, g dbSNP:376541815
627 627 a, g dbSNP:760621801
628 628 c, t dbSNP:773277281
631 631 a, g dbSNP:771669017
634 634 -, a dbSNP:397515517
635 635 -, a dbSNP:587776556
638 638 c, t dbSNP:775113388
659 659 c, t dbSNP:370003137
681 681 a, t dbSNP:141965690
684 684 a, c dbSNP:777230877
686 686 a, g dbSNP:771294247
689 689 g, t dbSNP:747126144
691 691 c, t dbSNP:147017317
692 692 a, c, g dbSNP:748437340
695 695 a, g dbSNP:779254873
697 697 c, t dbSNP:756012521
700 700 a, t dbSNP:2255543
703 703 a, c dbSNP:377043265
712 712 c, g dbSNP:757080944
720 720 a, c dbSNP:35702995
723 723 g, t dbSNP:770398071
748 748 a, g dbSNP:776344570
750 750 a, g dbSNP:767089050
751 751 a, g dbSNP:766714128
762 762 a, t dbSNP:143267384
767 767 a, c dbSNP:559502955
773 773 a, g dbSNP:772663207
774 774 c, t dbSNP:762006383
787 787 a, g dbSNP:774514092
800 800 a, c dbSNP:201753011
801 801 c, g dbSNP:368344978
803 803 c, g dbSNP:755734596
807 807 c, t dbSNP:569846003
809 809 a, c dbSNP:768059703
819 819 a, g, t dbSNP:752153829
820 820 c, g, t dbSNP:149165166
825 825 c, t dbSNP:544956641
827 827 a, g dbSNP:564979861
828 828 a, c, g dbSNP:374473331
832 832 c, t dbSNP:140977117
833 833 a, g dbSNP:759824026
838 838 c, g dbSNP:776824088
846 846 a, g dbSNP:771906714
847 847 a, t dbSNP:747904200
850 850 g, t dbSNP:774183442
856 856 a, t dbSNP:768585616
857 857 a, g dbSNP:748757618
869 869 c, g dbSNP:779549148
885 885 c, t dbSNP:755680655
906 906 a, g dbSNP:768481821
909 909 c, t dbSNP:541367417
910 910 a, c, g dbSNP:777846799
917 917 -, g dbSNP:397515346
918 918 a, g dbSNP:775274569
927 927 c, t dbSNP:769475625
933 933 c, t dbSNP:375396766
934 934 a, g, t dbSNP:2293734
941 941 a, g dbSNP:28941783
947 947 c, t dbSNP:140543217
955 955 c, t dbSNP:754865203
957 957 a, g dbSNP:556610453
961 961 c, g, t dbSNP:146878140
962 962 a, g dbSNP:375283568
964 964 c, g dbSNP:780173554
967 967 g, t dbSNP:756134838
969 969 -, g dbSNP:755342871
985 985 a, g dbSNP:536732498
990 990 a, g dbSNP:372293676
991 991 a, t dbSNP:762831007
1001 1001 a, g dbSNP:368256121
1003 1003 c, t dbSNP:765270269
1009 1009 g, t dbSNP:759615683
1019 1019 a, c, t dbSNP:756255206
1027 1027 c, g dbSNP:750428921
1047 1047 c, t dbSNP:781491692
1052 1052 a, g dbSNP:757355921
1053 1053 a, g dbSNP:199536408
1071 1071 a, g dbSNP:751827206
1072 1072 c, t dbSNP:765182260
1074 1074 a, g dbSNP:754802510
1077 1077 c, t dbSNP:753871493
1081 1081 c, t dbSNP:766190144
1091 1091 c, t dbSNP:760257697
1094 1094 a, g dbSNP:372084813
1099 1099 a, t dbSNP:750823780
1107 1107 c, t dbSNP:767201131
1112 1112 -, g dbSNP:786204662
1120 1120 c, g dbSNP:779777032
1123 1123 g, t dbSNP:755921083
1126 1126 c, g dbSNP:143942333
1128 1128 a, g dbSNP:750373701
1131 1131 a, c dbSNP:781152915
1133 1133 c, t dbSNP:756789146
1134 1134 a, c, g dbSNP:562853291
1144 1144 a, g dbSNP:139840318
1145 1145 a, g dbSNP:763742160
1146 1146 c, t dbSNP:762662465
1147 1147 a, g dbSNP:752381605
1148 1148 c, t dbSNP:28942100
1149 1149 c, g dbSNP:759918122
1152 1152 c, t dbSNP:534927553
1162 1162 c, t dbSNP:771518151
1171 1171 a, c dbSNP:147204182
1179 1179 c, t dbSNP:773355345
1186 1186 c, t dbSNP:772272120
1190 1190 a, g dbSNP:367751899
1191 1191 c, t dbSNP:779169911
1192 1192 a, g dbSNP:769516009
1208 1208 c, g dbSNP:745635477
1212 1212 a, g dbSNP:781011621
1216 1216 a, g dbSNP:375952822
1219 1219 g, t dbSNP:751495906
1222 1222 c, t dbSNP:777323362
1225 1225 c, t dbSNP:747764475
1227 1227 c, t dbSNP:752334034
1228 1228 c, g dbSNP:764907056
1233 1233 a, c dbSNP:759843592
1245 1245 a, c dbSNP:757937852
1246 1246 a, g dbSNP:747648606
1253 1253 a, g dbSNP:778448610
1254 1254 c, t dbSNP:554816026
1258 1258 a, g dbSNP:753425386
1265 1265 c, t dbSNP:766904253
1266 1266 a, g dbSNP:756522409
1267 1267 c, t dbSNP:758567130
1268 1268 a, g dbSNP:120074174
1282 1282 c, t dbSNP:761819710
1284 1284 a, g dbSNP:774431689
1285 1285 c, t dbSNP:764226435
1289 1289 c, t dbSNP:763294945
1292 1292 a, g dbSNP:370920635
1295 1295 a, c, t dbSNP:200382812
1297 1297 a, g dbSNP:773158062
1300 1300 a, g dbSNP:771989878
1307 1307 a, g dbSNP:747620917
1318 1318 c, g dbSNP:537736274
1333 1333 a, c dbSNP:754428438
1338 1338 a, c dbSNP:148641817
1355 1355 a, g dbSNP:372420052
1359 1359 g, t dbSNP:120074170
1365 1365 a, c dbSNP:752148612
1375 1375 c, t dbSNP:144158342
1379 1379 c, t dbSNP:143556739
1380 1380 a, g dbSNP:752719362
1382 1382 c, t dbSNP:765452552
1383 1383 c, g dbSNP:201529624
1404 1404 a, t dbSNP:754050823
1406 1406 g, t dbSNP:199927284
1414 1414 c, t dbSNP:761478895
1422 1422 a, g dbSNP:774374625
1423 1423 a, g dbSNP:768426419
1424 1424 -, g dbSNP:34214309
1426 1426 a, g dbSNP:745825659
1427 1427 a, g dbSNP:762564483
1429 1429 a, g dbSNP:376724242
1430 1430 -, g dbSNP:766354352
1433 1433 a, g dbSNP:769424424
1436 1436 a, g dbSNP:143396290
1439 1439 a, g dbSNP:372136565
1450 1450 g, t dbSNP:120074171
1452 1452 c, t dbSNP:771360138
1453 1453 c, t dbSNP:747288180
1464 1464 a, g dbSNP:778359542
1468 1468 a, t dbSNP:763050404
1469 1469 c, t dbSNP:390182
1482 1482 c, t dbSNP:530168427
1499 1499 a, c dbSNP:182166673
1531 1531 g, t dbSNP:190807395
1559 1559 a, g dbSNP:530704033
1620 1620 c, t dbSNP:564847483
1637 1637 c, t dbSNP:185160699
1673 1673 c, g dbSNP:572725686
1679 1679 a, t dbSNP:769469436
1680 1680 a, t dbSNP:759077394
1693 1693 c, t dbSNP:55998348
1696 1696 a, g dbSNP:375688145
1697 1697 a, t dbSNP:770435542
1712 1712 c, t dbSNP:747407858
1716 1716 c, t dbSNP:773519116
1718 1718 a, t dbSNP:772415605

Target ORF information:

RefSeq Version XM_005247413
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005247413

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGG GGCCAATGGC
TTGGCCAATC CTCGTGATTT CTTGATACCC ATTGCCTGGT ATGAGGATCG CCAAGTACCA
GGTGGTTACA CGGTCATTAA TAAATACCAG GGCAAGCTGT TTGCTGCCAA ACAGGATGTC
TCCCCGTTCA ATGTTGTGGC CTGGCACGGG AATTATACAC CCTACAAGTA CAACCTGAAG
AATTTCATGG TTATCAACTC AGTGGCCTTT GACCATGCAG ACCCATCCAT TTTCACAGTA
TTGACTGCTA AGTCTGTCCG CCCTGGAGTG GCCATTGCTG ATTTTGTCAT CTTCCCACCT
CGATGGGGGG TTGCTGATAA GACCTTCAGG CCTCCTTATT ACCATAATCT AGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58205
Accession Version XM_011512746.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 885bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product homogentisate 1,2-dioxygenase isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005612.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGG GGCCAATGGC
TTGGCCAATC CTCGTGATTT CTTGATACCC ATTGCCTGGT ATGAGGATCG CCAAGTACCA
GGTGGTTACA CGGTCATTAA TAAATACCAG GGCAAGCTGT TTGCTGCCAA ACAGGATGTC
TCCCCGTTCA ATGTTGTGGC CTGGCACGGG AATTATACAC CCTACAAGTA CAACCTGAAG
AATTTCATGG TTATCAACTC AGTGGCCTTT GACCATGCAA TCTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011511048.1
CDS461..1345
Misc Feature(1)467..>1339(+)
Translation
Position Chain Variation Link
9 9 g, t dbSNP:142298361
11 11 a, c dbSNP:2733824
15 15 g, t dbSNP:541656880
78 78 c, g dbSNP:148732655
94 94 c, g dbSNP:555576732
122 122 c, t dbSNP:2733829
164 164 a, g dbSNP:761807054
240 240 a, g dbSNP:535973482
243 243 a, g dbSNP:553200191
254 254 a, g dbSNP:147272996
260 260 a, g dbSNP:55661952
280 280 -, c dbSNP:397875516
298 298 c, t dbSNP:556156265
322 322 a, t dbSNP:539301020
324 324 a, g dbSNP:570423502
331 331 c, g dbSNP:760347090
335 335 g, t dbSNP:547327873
341 341 a, g dbSNP:191643714
353 353 c, t dbSNP:568270873
361 361 a, g dbSNP:547960368
405 405 g, t dbSNP:371878850
412 412 c, t dbSNP:747957946
419 419 a, g dbSNP:778619933
421 421 c, t dbSNP:768585000
435 435 a, g dbSNP:531449155
442 442 c, t dbSNP:376849236
443 443 a, g dbSNP:755624842
444 444 c, t dbSNP:749915521
448 448 c, g dbSNP:775087041
450 450 a, g dbSNP:371972791
457 457 a, g dbSNP:368515284
468 468 a, c dbSNP:200412910
471 471 a, t dbSNP:786204422
478 478 c, t dbSNP:769728875
484 484 c, t dbSNP:745386219
487 487 a, t dbSNP:780784207
492 492 a, g dbSNP:370453859
499 499 a, g dbSNP:751317891
500 500 c, t dbSNP:777547119
509 509 a, g dbSNP:758729926
513 513 a, g dbSNP:753068271
514 514 a, t dbSNP:765687351
518 518 c, t dbSNP:755454185
519 519 a, g dbSNP:753865195
530 530 c, t dbSNP:766513107
540 540 a, c dbSNP:760729411
545 545 c, g dbSNP:773469019
547 547 c, g dbSNP:767692484
557 557 c, g dbSNP:775575402
560 560 a, g dbSNP:770953681
570 570 a, t dbSNP:530032005
571 571 c, t dbSNP:772792095
576 576 a, t dbSNP:771625523
579 579 a, g dbSNP:747899770
580 580 c, t dbSNP:369064890
584 584 g, t dbSNP:769112916
585 585 a, c dbSNP:373921680
589 589 a, g dbSNP:142789485
597 597 a, g dbSNP:756560798
598 598 a, g, t dbSNP:374495094
600 600 a, c, t dbSNP:369517993
601 601 a, g dbSNP:541268108
602 602 g, t dbSNP:138846036
617 617 c, t dbSNP:759435977
618 618 a, g dbSNP:200808744
621 621 a, g dbSNP:376541815
627 627 a, g dbSNP:760621801
628 628 c, t dbSNP:773277281
631 631 a, g dbSNP:771669017
634 634 -, a dbSNP:397515517
635 635 -, a dbSNP:587776556
638 638 c, t dbSNP:775113388
659 659 c, t dbSNP:370003137
681 681 a, t dbSNP:141965690
684 684 a, c dbSNP:777230877
686 686 a, g dbSNP:771294247
689 689 g, t dbSNP:747126144
691 691 c, t dbSNP:147017317
692 692 a, c, g dbSNP:748437340
695 695 a, g dbSNP:779254873
697 697 c, t dbSNP:756012521
700 700 a, t dbSNP:2255543
703 703 a, c dbSNP:377043265
712 712 c, g dbSNP:757080944
720 720 a, c dbSNP:35702995
723 723 g, t dbSNP:770398071
748 748 a, g dbSNP:776344570
750 750 a, g dbSNP:767089050
751 751 a, g dbSNP:766714128
762 762 a, t dbSNP:143267384
767 767 a, c dbSNP:559502955
773 773 a, g dbSNP:772663207
774 774 c, t dbSNP:762006383
787 787 a, g dbSNP:774514092
800 800 a, c dbSNP:201753011
801 801 c, g dbSNP:368344978
803 803 c, g dbSNP:755734596
807 807 c, t dbSNP:569846003
809 809 a, c dbSNP:768059703
819 819 a, g, t dbSNP:752153829
820 820 c, g, t dbSNP:149165166
825 825 c, t dbSNP:544956641
827 827 a, g dbSNP:564979861
828 828 a, c, g dbSNP:374473331
832 832 c, t dbSNP:140977117
833 833 a, g dbSNP:759824026
838 838 c, g dbSNP:776824088
846 846 a, g dbSNP:771906714
847 847 a, t dbSNP:747904200
850 850 g, t dbSNP:774183442
856 856 a, t dbSNP:768585616
857 857 a, g dbSNP:748757618
869 869 c, g dbSNP:779549148
885 885 c, t dbSNP:755680655
906 906 a, g dbSNP:768481821
909 909 c, t dbSNP:541367417
910 910 a, c, g dbSNP:777846799
917 917 -, g dbSNP:397515346
918 918 a, g dbSNP:775274569
927 927 c, t dbSNP:769475625
933 933 c, t dbSNP:375396766
934 934 a, g, t dbSNP:2293734
941 941 a, g dbSNP:28941783
947 947 c, t dbSNP:140543217
955 955 c, t dbSNP:754865203
957 957 a, g dbSNP:556610453
961 961 c, g, t dbSNP:146878140
962 962 a, g dbSNP:375283568
964 964 c, g dbSNP:780173554
967 967 g, t dbSNP:756134838
969 969 -, g dbSNP:755342871
985 985 a, g dbSNP:536732498
990 990 a, g dbSNP:372293676
991 991 a, t dbSNP:762831007
1001 1001 a, g dbSNP:368256121
1003 1003 c, t dbSNP:765270269
1009 1009 g, t dbSNP:759615683
1019 1019 a, c, t dbSNP:756255206
1027 1027 c, g dbSNP:750428921
1047 1047 c, t dbSNP:781491692
1052 1052 a, g dbSNP:757355921
1053 1053 a, g dbSNP:199536408
1071 1071 a, g dbSNP:751827206
1072 1072 c, t dbSNP:765182260
1074 1074 a, g dbSNP:754802510
1077 1077 c, t dbSNP:753871493
1081 1081 c, t dbSNP:766190144
1091 1091 c, t dbSNP:760257697
1094 1094 a, g dbSNP:372084813
1099 1099 a, t dbSNP:750823780
1107 1107 c, t dbSNP:767201131
1112 1112 -, g dbSNP:786204662
1120 1120 c, g dbSNP:779777032
1123 1123 g, t dbSNP:755921083
1126 1126 c, g dbSNP:143942333
1128 1128 a, g dbSNP:750373701
1131 1131 a, c dbSNP:781152915
1133 1133 c, t dbSNP:756789146
1134 1134 a, c, g dbSNP:562853291
1144 1144 a, g dbSNP:139840318
1145 1145 a, g dbSNP:763742160
1146 1146 c, t dbSNP:762662465
1147 1147 a, g dbSNP:752381605
1148 1148 c, t dbSNP:28942100
1149 1149 c, g dbSNP:759918122
1152 1152 c, t dbSNP:534927553
1162 1162 c, t dbSNP:771518151
1171 1171 a, c dbSNP:147204182
1179 1179 c, t dbSNP:773355345
1186 1186 c, t dbSNP:772272120
1190 1190 a, g dbSNP:367751899
1191 1191 c, t dbSNP:779169911
1192 1192 a, g dbSNP:769516009
1208 1208 c, g dbSNP:745635477
1212 1212 a, g dbSNP:781011621
1216 1216 a, g dbSNP:375952822
1219 1219 g, t dbSNP:751495906
1222 1222 c, t dbSNP:777323362
1225 1225 c, t dbSNP:747764475
1227 1227 c, t dbSNP:752334034
1228 1228 c, g dbSNP:764907056
1233 1233 a, c dbSNP:759843592
1245 1245 a, c dbSNP:757937852
1246 1246 a, g dbSNP:747648606
1253 1253 a, g dbSNP:778448610
1254 1254 c, t dbSNP:554816026
1258 1258 a, g dbSNP:753425386
1265 1265 c, t dbSNP:766904253
1266 1266 a, g dbSNP:756522409
1267 1267 c, t dbSNP:758567130
1268 1268 a, g dbSNP:120074174
1282 1282 c, t dbSNP:761819710
1284 1284 a, g dbSNP:774431689
1285 1285 c, t dbSNP:764226435
1289 1289 c, t dbSNP:763294945
1292 1292 a, g dbSNP:370920635
1295 1295 a, c, t dbSNP:200382812
1297 1297 a, g dbSNP:773158062
1300 1300 a, g dbSNP:771989878
1307 1307 a, g dbSNP:747620917
1318 1318 c, g dbSNP:537736274
1333 1333 a, c dbSNP:754428438
1338 1338 a, c dbSNP:148641817
1341 1341 a, t dbSNP:763050404
1342 1342 c, t dbSNP:390182
1355 1355 c, t dbSNP:530168427
1372 1372 a, c dbSNP:182166673
1404 1404 g, t dbSNP:190807395
1432 1432 a, g dbSNP:530704033
1493 1493 c, t dbSNP:564847483
1510 1510 c, t dbSNP:185160699
1546 1546 c, g dbSNP:572725686
1552 1552 a, t dbSNP:769469436
1553 1553 a, t dbSNP:759077394
1566 1566 c, t dbSNP:55998348
1569 1569 a, g dbSNP:375688145
1570 1570 a, t dbSNP:770435542
1585 1585 c, t dbSNP:747407858
1589 1589 c, t dbSNP:773519116
1591 1591 a, t dbSNP:772415605

Target ORF information:

RefSeq Version XM_011512746
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011512746

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGG GGCCAATGGC
TTGGCCAATC CTCGTGATTT CTTGATACCC ATTGCCTGGT ATGAGGATCG CCAAGTACCA
GGTGGTTACA CGGTCATTAA TAAATACCAG GGCAAGCTGT TTGCTGCCAA ACAGGATGTC
TCCCCGTTCA ATGTTGTGGC CTGGCACGGG AATTATACAC CCTACAAGTA CAACCTGAAG
AATTTCATGG TTATCAACTC AGTGGCCTTT GACCATGCAA TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37914
Accession Version XM_005247414.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 702bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product homogentisate 1,2-dioxygenase isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005612.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578807337. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGA TTCCTTGGGT
CTTCCTAAAA GGTACCAGAC TGGAAATGCA GTGAATCTTT GA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005247471.1
CDS461..1162
Misc Feature(1)467..>1141(+)
Translation
Position Chain Variation Link
9 9 g, t dbSNP:142298361
11 11 a, c dbSNP:2733824
15 15 g, t dbSNP:541656880
78 78 c, g dbSNP:148732655
94 94 c, g dbSNP:555576732
122 122 c, t dbSNP:2733829
164 164 a, g dbSNP:761807054
240 240 a, g dbSNP:535973482
243 243 a, g dbSNP:553200191
254 254 a, g dbSNP:147272996
260 260 a, g dbSNP:55661952
280 280 -, c dbSNP:397875516
298 298 c, t dbSNP:556156265
322 322 a, t dbSNP:539301020
324 324 a, g dbSNP:570423502
331 331 c, g dbSNP:760347090
335 335 g, t dbSNP:547327873
341 341 a, g dbSNP:191643714
353 353 c, t dbSNP:568270873
361 361 a, g dbSNP:547960368
405 405 g, t dbSNP:371878850
412 412 c, t dbSNP:747957946
419 419 a, g dbSNP:778619933
421 421 c, t dbSNP:768585000
435 435 a, g dbSNP:531449155
442 442 c, t dbSNP:376849236
443 443 a, g dbSNP:755624842
444 444 c, t dbSNP:749915521
448 448 c, g dbSNP:775087041
450 450 a, g dbSNP:371972791
457 457 a, g dbSNP:368515284
468 468 a, c dbSNP:200412910
471 471 a, t dbSNP:786204422
478 478 c, t dbSNP:769728875
484 484 c, t dbSNP:745386219
487 487 a, t dbSNP:780784207
492 492 a, g dbSNP:370453859
499 499 a, g dbSNP:751317891
500 500 c, t dbSNP:777547119
509 509 a, g dbSNP:758729926
513 513 a, g dbSNP:753068271
514 514 a, t dbSNP:765687351
518 518 c, t dbSNP:755454185
519 519 a, g dbSNP:753865195
530 530 c, t dbSNP:766513107
540 540 a, c dbSNP:760729411
545 545 c, g dbSNP:773469019
547 547 c, g dbSNP:767692484
557 557 c, g dbSNP:775575402
560 560 a, g dbSNP:770953681
570 570 a, t dbSNP:530032005
571 571 c, t dbSNP:772792095
576 576 a, t dbSNP:771625523
579 579 a, g dbSNP:747899770
580 580 c, t dbSNP:369064890
584 584 g, t dbSNP:769112916
585 585 a, c dbSNP:373921680
589 589 a, g dbSNP:142789485
597 597 a, g dbSNP:756560798
598 598 a, g, t dbSNP:374495094
600 600 a, c, t dbSNP:369517993
601 601 a, g dbSNP:541268108
602 602 g, t dbSNP:138846036
617 617 c, t dbSNP:759435977
618 618 a, g dbSNP:200808744
621 621 a, g dbSNP:376541815
627 627 a, g dbSNP:760621801
628 628 c, t dbSNP:773277281
631 631 a, g dbSNP:771669017
634 634 -, a dbSNP:397515517
635 635 -, a dbSNP:587776556
638 638 c, t dbSNP:775113388
659 659 c, t dbSNP:370003137
681 681 a, t dbSNP:141965690
684 684 a, c dbSNP:777230877
686 686 a, g dbSNP:771294247
689 689 g, t dbSNP:747126144
691 691 c, t dbSNP:147017317
692 692 a, c, g dbSNP:748437340
695 695 a, g dbSNP:779254873
697 697 c, t dbSNP:756012521
700 700 a, t dbSNP:2255543
703 703 a, c dbSNP:377043265
712 712 c, g dbSNP:757080944
720 720 a, c dbSNP:35702995
723 723 g, t dbSNP:770398071
748 748 a, g dbSNP:776344570
750 750 a, g dbSNP:767089050
751 751 a, g dbSNP:766714128
762 762 a, t dbSNP:143267384
767 767 a, c dbSNP:559502955
773 773 a, g dbSNP:772663207
774 774 c, t dbSNP:762006383
787 787 a, g dbSNP:774514092
800 800 a, c dbSNP:201753011
801 801 c, g dbSNP:368344978
803 803 c, g dbSNP:755734596
807 807 c, t dbSNP:569846003
809 809 a, c dbSNP:768059703
819 819 a, g, t dbSNP:752153829
820 820 c, g, t dbSNP:149165166
825 825 c, t dbSNP:544956641
827 827 a, g dbSNP:564979861
828 828 a, c, g dbSNP:374473331
832 832 c, t dbSNP:140977117
833 833 a, g dbSNP:759824026
838 838 c, g dbSNP:776824088
846 846 a, g dbSNP:771906714
847 847 a, t dbSNP:747904200
850 850 g, t dbSNP:774183442
856 856 a, t dbSNP:768585616
857 857 a, g dbSNP:748757618
869 869 c, g dbSNP:779549148
885 885 c, t dbSNP:755680655
906 906 a, g dbSNP:768481821
909 909 c, t dbSNP:541367417
910 910 a, c, g dbSNP:777846799
917 917 -, g dbSNP:397515346
918 918 a, g dbSNP:775274569
927 927 c, t dbSNP:769475625
933 933 c, t dbSNP:375396766
934 934 a, g, t dbSNP:2293734
941 941 a, g dbSNP:28941783
947 947 c, t dbSNP:140543217
955 955 c, t dbSNP:754865203
957 957 a, g dbSNP:556610453
961 961 c, g, t dbSNP:146878140
962 962 a, g dbSNP:375283568
964 964 c, g dbSNP:780173554
967 967 g, t dbSNP:756134838
969 969 -, g dbSNP:755342871
985 985 a, g dbSNP:536732498
990 990 a, g dbSNP:372293676
991 991 a, t dbSNP:762831007
1001 1001 a, g dbSNP:368256121
1003 1003 c, t dbSNP:765270269
1009 1009 g, t dbSNP:759615683
1019 1019 a, c, t dbSNP:756255206
1027 1027 c, g dbSNP:750428921
1047 1047 c, t dbSNP:781491692
1052 1052 a, g dbSNP:757355921
1053 1053 a, g dbSNP:199536408
1071 1071 a, g dbSNP:751827206
1072 1072 c, t dbSNP:765182260
1074 1074 a, g dbSNP:754802510
1077 1077 c, t dbSNP:753871493
1081 1081 c, t dbSNP:766190144
1091 1091 c, t dbSNP:760257697
1094 1094 a, g dbSNP:372084813
1099 1099 a, t dbSNP:750823780
1107 1107 c, t dbSNP:767201131
1127 1127 a, g dbSNP:774032695
1131 1131 a, g dbSNP:1370742
1152 1152 c, t dbSNP:545096337
1168 1168 a, g dbSNP:775652460
1174 1174 a, t dbSNP:770192944
1187 1187 g, t dbSNP:770892279
1201 1201 a, g dbSNP:2075504
1202 1202 a, g dbSNP:541803197
1225 1225 c, t dbSNP:576109490
1233 1233 c, t dbSNP:557341939
1233 1233 -, t dbSNP:758465592
1247 1247 -, a, t dbSNP:377387456
1248 1248 c, g dbSNP:746421132
1249 1249 a, g dbSNP:777174037
1252 1252 c, g dbSNP:770960854
1258 1258 a, g dbSNP:747049467
1262 1262 g, t dbSNP:778165782
1263 1263 a, g dbSNP:758638841
1264 1264 c, t dbSNP:537764161

Target ORF information:

RefSeq Version XM_005247414
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005247414

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGA TTCCTTGGGT
CTTCCTAAAA GGTACCAGAC TGGAAATGCA GTGAATCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20740
Accession Version NM_000187.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1338bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product homogentisate 1,2-dioxygenase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB231845.1, AK313563.1, AC133474.2 and AA861603.1. This sequence is a reference standard in the RefSeqGene project. On May 13, 2010 this sequence version replaced gi:115527116. Summary: This gene encodes the enzyme homogentisate 1,2 dioxygenase. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria.[provided by RefSeq, May 2010]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK313563.1, AF045167.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGG GGCCAATGGC
TTGGCCAATC CTCGTGATTT CTTGATACCC ATTGCCTGGT ATGAGGATCG CCAAGTACCA
GGTGGTTACA CGGTCATTAA TAAATACCAG GGCAAGCTGT TTGCTGCCAA ACAGGATGTC
TCCCCGTTCA ATGTTGTGGC CTGGCACGGG AATTATACAC CCTACAAGTA CAACCTGAAG
AATTTCATGG TTATCAACTC AGTGGCCTTT GACCATGCAG ACCCATCCAT TTTCACAGTA
TTGACTGCTA AGTCTGTCCG CCCTGGAGTG GCCATTGCTG ATTTTGTCAT CTTCCCACCT
CGATGGGGGG TTGCTGATAA GACCTTCAGG CCTCCTTATT ACCATAGGAA CTGCATGAGT
GAGTTCATGG GACTCATCCG AGGTCACTAT GAGGCAAAGC AAGGTGGGTT CCTGCCAGGG
GGAGGGAGTC TACACAGCAC AATGACCCCC CATGGACCTG ATGCTGACTG CTTTGAGAAG
GCCAGCAAGG TCAAGCTGGC ACCTGAGAGG ATTGCCGATG GCACCATGGC ATTTATGTTT
GAATCATCTT TAAGTCTGGC GGTCACAAAG TGGGGACTCA AGGCCTCCAG GTGTTTGGAT
GAGAACTACC ACAAGTGCTG GGAGCCACTC AAGAGCCACT TCACTCCCAA CTCCAGGAAC
CCAGCAGAAC CTAATTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000178.2
CDS461..1798
Misc Feature(1)446..448(+)
Misc Feature(2)467..1762(+)
Misc Feature(3)752..754(+)
Exon (1)1..475
Gene:HGD
Gene Synonym:
Exon (2)476..547
Gene:HGD
Gene Synonym:
Exon (3)548..636
Gene:HGD
Gene Synonym:
Exon (4)637..742
Gene:HGD
Gene Synonym:
Exon (5)743..802
Gene:HGD
Gene Synonym:
Exon (6)803..894
Gene:HGD
Gene Synonym:
Exon (7)895..929
Gene:HGD
Gene Synonym:
Exon (8)930..1009
Gene:HGD
Gene Synonym:
Exon (9)1010..1109
Gene:HGD
Gene Synonym:
Exon (10)1110..1234
Gene:HGD
Gene Synonym:
Exon (11)1235..1339
Gene:HGD
Gene Synonym:
Exon (12)1340..1466
Gene:HGD
Gene Synonym:
Exon (13)1467..1648
Gene:HGD
Gene Synonym:
Exon (14)1649..2010
Gene:HGD
Gene Synonym:
Translation
Position Chain Variation Link
9 9 g, t dbSNP:142298361
11 11 a, c dbSNP:2733824
15 15 g, t dbSNP:541656880
78 78 c, g dbSNP:148732655
94 94 c, g dbSNP:555576732
122 122 c, t dbSNP:2733829
164 164 a, g dbSNP:761807054
240 240 a, g dbSNP:535973482
243 243 a, g dbSNP:553200191
254 254 a, g dbSNP:147272996
260 260 a, g dbSNP:55661952
280 280 -, c dbSNP:397875516
298 298 c, t dbSNP:556156265
322 322 a, t dbSNP:539301020
324 324 a, g dbSNP:570423502
331 331 c, g dbSNP:760347090
335 335 g, t dbSNP:547327873
341 341 a, g dbSNP:191643714
353 353 c, t dbSNP:568270873
361 361 a, g dbSNP:547960368
405 405 g, t dbSNP:371878850
412 412 c, t dbSNP:747957946
419 419 a, g dbSNP:778619933
421 421 c, t dbSNP:768585000
435 435 a, g dbSNP:531449155
442 442 c, t dbSNP:376849236
443 443 a, g dbSNP:755624842
444 444 c, t dbSNP:749915521
448 448 c, g dbSNP:775087041
450 450 a, g dbSNP:371972791
457 457 a, g dbSNP:368515284
468 468 a, c dbSNP:200412910
471 471 a, t dbSNP:786204422
478 478 c, t dbSNP:769728875
484 484 c, t dbSNP:745386219
487 487 a, t dbSNP:780784207
492 492 a, g dbSNP:370453859
499 499 a, g dbSNP:751317891
500 500 c, t dbSNP:777547119
509 509 a, g dbSNP:758729926
513 513 a, g dbSNP:753068271
514 514 a, t dbSNP:765687351
518 518 c, t dbSNP:755454185
519 519 a, g dbSNP:753865195
530 530 c, t dbSNP:766513107
540 540 a, c dbSNP:760729411
545 545 c, g dbSNP:773469019
547 547 c, g dbSNP:767692484
557 557 c, g dbSNP:775575402
560 560 a, g dbSNP:770953681
570 570 a, t dbSNP:530032005
571 571 c, t dbSNP:772792095
576 576 a, t dbSNP:771625523
579 579 a, g dbSNP:747899770
580 580 c, t dbSNP:369064890
584 584 g, t dbSNP:769112916
585 585 a, c dbSNP:373921680
589 589 a, g dbSNP:142789485
597 597 a, g dbSNP:756560798
598 598 a, g, t dbSNP:374495094
600 600 a, c, t dbSNP:369517993
601 601 a, g dbSNP:541268108
602 602 g, t dbSNP:138846036
617 617 c, t dbSNP:759435977
618 618 a, g dbSNP:200808744
621 621 a, g dbSNP:376541815
627 627 a, g dbSNP:760621801
628 628 c, t dbSNP:773277281
631 631 a, g dbSNP:771669017
634 634 -, a dbSNP:397515517
635 635 -, a dbSNP:587776556
638 638 c, t dbSNP:775113388
659 659 c, t dbSNP:370003137
681 681 a, t dbSNP:141965690
684 684 a, c dbSNP:777230877
686 686 a, g dbSNP:771294247
689 689 g, t dbSNP:747126144
691 691 c, t dbSNP:147017317
692 692 a, c, g dbSNP:748437340
695 695 a, g dbSNP:779254873
697 697 c, t dbSNP:756012521
700 700 a, t dbSNP:2255543
703 703 a, c dbSNP:377043265
712 712 c, g dbSNP:757080944
720 720 a, c dbSNP:35702995
723 723 g, t dbSNP:770398071
748 748 a, g dbSNP:776344570
750 750 a, g dbSNP:767089050
751 751 a, g dbSNP:766714128
762 762 a, t dbSNP:143267384
767 767 a, c dbSNP:559502955
773 773 a, g dbSNP:772663207
774 774 c, t dbSNP:762006383
787 787 a, g dbSNP:774514092
800 800 a, c dbSNP:201753011
801 801 c, g dbSNP:368344978
803 803 c, g dbSNP:755734596
807 807 c, t dbSNP:569846003
809 809 a, c dbSNP:768059703
819 819 a, g, t dbSNP:752153829
820 820 c, g, t dbSNP:149165166
825 825 c, t dbSNP:544956641
827 827 a, g dbSNP:564979861
828 828 a, c, g dbSNP:374473331
832 832 c, t dbSNP:140977117
833 833 a, g dbSNP:759824026
838 838 c, g dbSNP:776824088
846 846 a, g dbSNP:771906714
847 847 a, t dbSNP:747904200
850 850 g, t dbSNP:774183442
856 856 a, t dbSNP:768585616
857 857 a, g dbSNP:748757618
869 869 c, g dbSNP:779549148
885 885 c, t dbSNP:755680655
906 906 a, g dbSNP:768481821
909 909 c, t dbSNP:541367417
910 910 a, c, g dbSNP:777846799
917 917 -, g dbSNP:397515346
918 918 a, g dbSNP:775274569
927 927 c, t dbSNP:769475625
933 933 c, t dbSNP:375396766
934 934 a, g, t dbSNP:2293734
941 941 a, g dbSNP:28941783
947 947 c, t dbSNP:140543217
955 955 c, t dbSNP:754865203
957 957 a, g dbSNP:556610453
961 961 c, g, t dbSNP:146878140
962 962 a, g dbSNP:375283568
964 964 c, g dbSNP:780173554
967 967 g, t dbSNP:756134838
969 969 -, g dbSNP:755342871
985 985 a, g dbSNP:536732498
990 990 a, g dbSNP:372293676
991 991 a, t dbSNP:762831007
1001 1001 a, g dbSNP:368256121
1003 1003 c, t dbSNP:765270269
1009 1009 g, t dbSNP:759615683
1019 1019 a, c, t dbSNP:756255206
1027 1027 c, g dbSNP:750428921
1047 1047 c, t dbSNP:781491692
1052 1052 a, g dbSNP:757355921
1053 1053 a, g dbSNP:199536408
1071 1071 a, g dbSNP:751827206
1072 1072 c, t dbSNP:765182260
1074 1074 a, g dbSNP:754802510
1077 1077 c, t dbSNP:753871493
1081 1081 c, t dbSNP:766190144
1091 1091 c, t dbSNP:760257697
1094 1094 a, g dbSNP:372084813
1099 1099 a, t dbSNP:750823780
1107 1107 c, t dbSNP:767201131
1112 1112 -, g dbSNP:786204662
1120 1120 c, g dbSNP:779777032
1123 1123 g, t dbSNP:755921083
1126 1126 c, g dbSNP:143942333
1128 1128 a, g dbSNP:750373701
1131 1131 a, c dbSNP:781152915
1133 1133 c, t dbSNP:756789146
1134 1134 a, c, g dbSNP:562853291
1144 1144 a, g dbSNP:139840318
1145 1145 a, g dbSNP:763742160
1146 1146 c, t dbSNP:762662465
1147 1147 a, g dbSNP:752381605
1148 1148 c, t dbSNP:28942100
1149 1149 c, g dbSNP:759918122
1152 1152 c, t dbSNP:534927553
1162 1162 c, t dbSNP:771518151
1171 1171 a, c dbSNP:147204182
1179 1179 c, t dbSNP:773355345
1186 1186 c, t dbSNP:772272120
1190 1190 a, g dbSNP:367751899
1191 1191 c, t dbSNP:779169911
1192 1192 a, g dbSNP:769516009
1208 1208 c, g dbSNP:745635477
1212 1212 a, g dbSNP:781011621
1216 1216 a, g dbSNP:375952822
1219 1219 g, t dbSNP:751495906
1222 1222 c, t dbSNP:777323362
1225 1225 c, t dbSNP:747764475
1227 1227 c, t dbSNP:752334034
1228 1228 c, g dbSNP:764907056
1233 1233 a, c dbSNP:759843592
1245 1245 a, c dbSNP:757937852
1246 1246 a, g dbSNP:747648606
1253 1253 a, g dbSNP:778448610
1254 1254 c, t dbSNP:554816026
1258 1258 a, g dbSNP:753425386
1265 1265 c, t dbSNP:766904253
1266 1266 a, g dbSNP:756522409
1267 1267 c, t dbSNP:758567130
1268 1268 a, g dbSNP:120074174
1282 1282 c, t dbSNP:761819710
1284 1284 a, g dbSNP:774431689
1285 1285 c, t dbSNP:764226435
1289 1289 c, t dbSNP:763294945
1292 1292 a, g dbSNP:370920635
1295 1295 a, c, t dbSNP:200382812
1297 1297 a, g dbSNP:773158062
1300 1300 a, g dbSNP:771989878
1307 1307 a, g dbSNP:747620917
1318 1318 c, g dbSNP:537736274
1333 1333 a, c dbSNP:754428438
1338 1338 a, c dbSNP:148641817
1355 1355 a, g dbSNP:372420052
1359 1359 g, t dbSNP:120074170
1365 1365 a, c dbSNP:752148612
1375 1375 c, t dbSNP:144158342
1379 1379 c, t dbSNP:143556739
1380 1380 a, g dbSNP:752719362
1382 1382 c, t dbSNP:765452552
1383 1383 c, g dbSNP:201529624
1404 1404 a, t dbSNP:754050823
1406 1406 g, t dbSNP:199927284
1414 1414 c, t dbSNP:761478895
1422 1422 a, g dbSNP:774374625
1423 1423 a, g dbSNP:768426419
1424 1424 -, g dbSNP:34214309
1426 1426 a, g dbSNP:745825659
1427 1427 a, g dbSNP:762564483
1429 1429 a, g dbSNP:376724242
1430 1430 -, g dbSNP:766354352
1433 1433 a, g dbSNP:769424424
1436 1436 a, g dbSNP:143396290
1439 1439 a, g dbSNP:372136565
1450 1450 g, t dbSNP:120074171
1452 1452 c, t dbSNP:771360138
1453 1453 c, t dbSNP:747288180
1464 1464 a, g dbSNP:778359542
1468 1468 -, g dbSNP:35952153
1474 1474 c, t dbSNP:748707886
1477 1477 g, t dbSNP:139501220
1479 1479 -, g dbSNP:762999434
1487 1487 a, c dbSNP:755078457
1489 1489 a, g dbSNP:778231396
1506 1506 a, g dbSNP:749274871
1520 1520 c, t dbSNP:780333531
1524 1524 a, g dbSNP:756222568
1529 1529 a, t dbSNP:750651924
1536 1536 c, t dbSNP:764037565
1538 1538 c, g dbSNP:368717991
1540 1540 a, g dbSNP:752713602
1541 1541 c, g dbSNP:765219004
1557 1557 a, g dbSNP:759101002
1562 1562 a, g dbSNP:120074173
1566 1566 a, c dbSNP:765912447
1570 1570 a, c dbSNP:760307527
1571 1571 -, c dbSNP:397515516
1572 1572 a, g dbSNP:120074172
1578 1578 c, t dbSNP:138558042
1588 1588 c, g dbSNP:150145204
1595 1595 c, g dbSNP:141753513
1612 1612 c, t dbSNP:774858137
1614 1614 a, c dbSNP:769091766
1617 1617 -, tggc dbSNP:772802378
1617 1617 c, t dbSNP:371538956
1622 1622 c, t dbSNP:780063156
1624 1624 c, t dbSNP:756204156
1632 1632 c, t dbSNP:746125474
1636 1636 c, t dbSNP:368805723
1637 1637 a, g dbSNP:758291793
1639 1639 c, t dbSNP:146482816
1644 1644 c, t dbSNP:765272112
1646 1646 a, g dbSNP:754940101
1648 1648 a, g dbSNP:753803362
1649 1649 a, g dbSNP:373918530
1651 1651 a, c, g dbSNP:137923025
1661 1661 c, g dbSNP:767159114
1666 1666 a, g dbSNP:140500816
1667 1667 c, t dbSNP:751197824
1680 1680 c, t dbSNP:764414572
1681 1681 a, g dbSNP:146206905
1688 1688 a, g dbSNP:775883622
1690 1690 g, t dbSNP:770278846
1697 1697 c, t dbSNP:370401523
1703 1703 a, g dbSNP:776919863
1716 1716 g, t dbSNP:771123717
1716 1716 -, t dbSNP:747809657
1723 1723 c, g dbSNP:747286602
1733 1733 a, c dbSNP:765596920
1737 1737 g, t dbSNP:768371363
1745 1745 c, g dbSNP:749059002
1749 1749 a, t dbSNP:779866537
1756 1756 c, t dbSNP:756009394
1765 1765 c, t dbSNP:571129573
1771 1771 a, c, t dbSNP:756737699
1774 1774 c, t dbSNP:751252758
1795 1795 c, t dbSNP:187614203
1796 1796 c, t dbSNP:143370662
1801 1801 -, c dbSNP:776190831
1805 1805 a, g dbSNP:753039865
1807 1807 a, c, t dbSNP:760180599
1815 1815 c, t dbSNP:777259020
1830 1830 a, g dbSNP:376051958
1840 1840 a, g dbSNP:374327803
1845 1845 a, c dbSNP:773324881
1862 1862 g, t dbSNP:762250068
1866 1866 a, g dbSNP:527905808
1920 1920 a, g dbSNP:562005702
1944 1944 a, g dbSNP:542446346
1950 1950 a, g dbSNP:542329785
1953 1953 a, g dbSNP:776810530
1957 1957 g, t dbSNP:531872369
2008 2008 a, g dbSNP:768955353

Target ORF information:

RefSeq Version NM_000187
Organism Homo sapiens (human)
Definition Homo sapiens homogentisate 1,2-dioxygenase (HGD), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000187

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGG GGCCAATGGC
TTGGCCAATC CTCGTGATTT CTTGATACCC ATTGCCTGGT ATGAGGATCG CCAAGTACCA
GGTGGTTACA CGGTCATTAA TAAATACCAG GGCAAGCTGT TTGCTGCCAA ACAGGATGTC
TCCCCGTTCA ATGTTGTGGC CTGGCACGGG AATTATACAC CCTACAAGTA CAACCTGAAG
AATTTCATGG TTATCAACTC AGTGGCCTTT GACCATGCAG ACCCATCCAT TTTCACAGTA
TTGACTGCTA AGTCTGTCCG CCCTGGAGTG GCCATTGCTG ATTTTGTCAT CTTCCCACCT
CGATGGGGGG TTGCTGATAA GACCTTCAGG CCTCCTTATT ACCATAGGAA CTGCATGAGT
GAGTTCATGG GACTCATCCG AGGTCACTAT GAGGCAAAGC AAGGTGGGTT CCTGCCAGGG
GGAGGGAGTC TACACAGCAC AATGACCCCC CATGGACCTG ATGCTGACTG CTTTGAGAAG
GCCAGCAAGG TCAAGCTGGC ACCTGAGAGG ATTGCCGATG GCACCATGGC ATTTATGTTT
GAATCATCTT TAAGTCTGGC GGTCACAAAG TGGGGACTCA AGGCCTCCAG GTGTTTGGAT
GAGAACTACC ACAAGTGCTG GGAGCCACTC AAGAGCCACT TCACTCCCAA CTCCAGGAAC
CCAGCAGAAC CTAATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.