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PLA2G2E cDNA ORF clone, Homo sapiens (human)

Gene Symbol PLA2G2E
Entrez Gene ID 30814
Full Name phospholipase A2, group IIE
Synonyms GIIE sPLA2, sPLA2-IIE
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1p36.13

Summary lac of sum

mRNA and Protein(s)

mRNA Protein Name
NM_014589 NP_055404 group IIE secretory phospholipase A2 precursor

KEGG
hsa00590 Arachidonic acid metabolism
hsa00564 Glycerophospholipid metabolism
hsa00592 alpha-Linolenic acid metabolism
hsa00565 Ether lipid metabolism
hsa00591 Linoleic acid metabolism
hsa04270 Vascular smooth muscle contraction
hsa01100 Metabolic pathways
hsa04972 Pancreatic secretion
hsa04975 Fat digestion and absorption
hsa04014 Ras signaling pathway
Reactome
R-HSA-1430728 Metabolism
R-HSA-556833 Metabolism of lipids and lipoproteins
R-HSA-1482801 Acyl chain remodelling of PS
R-HSA-1482922 Acyl chain remodelling of PI
R-HSA-1483166 Synthesis of PA
R-HSA-1482839 Acyl chain remodelling of PE
R-HSA-1482925 Acyl chain remodelling of PG
R-HSA-1482788 Acyl chain remodelling of PC
R-HSA-1483257 Phospholipid metabolism
R-HSA-1483206 Glycerophospholipid biosynthesis
BIOCYC
HUMAN_LIPASYN-PWY phospholipases


Homo sapiens (human) PLA2G2E NP_055404.1
Pan troglodytes (chimpanzee) PLA2G2E XP_001163677.1
Macaca mulatta (Rhesus monkey) PLA2G2E XP_001094364.1
Canis lupus familiaris (dog) PLA2G2E XP_544525.3
Bos taurus (cattle) PLA2G2E NP_001179015.1
Mus musculus (house mouse) Pla2g2e NP_036174.1
Gallus gallus (chicken) PLA2G2E NP_001171878.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC100496855 XP_002938654.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC101735122 XP_004920461.1

Component

ID Name Evidence
GO:0005576 extracellular region IEA

Function

ID Name Evidence
GO:0004623 phospholipase A2 activity IEA
GO:0005509 calcium ion binding IEA
GO:0016787 hydrolase activity IEA

Process

ID Name Evidence
GO:0006644 phospholipid metabolic process IEA
GO:0006954 inflammatory response TAS
GO:0016042 lipid catabolic process IEA

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PLA2G2E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLA2G2E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu01793
NM_014589 Homo sapiens phospholipase A2, group IIE (PLA2G2E), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $49.50
$99.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu01793
Clone ID Related Accession (Same CDS sequence) NM_014589
Accession Version NM_014589.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 429bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 20-FEB-2014
Organism Homo sapiens (human)
Product group IIE secretory phospholipase A2 precursor
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF189279.1 and AL358253.16. On Nov 17, 2012 this sequence version replaced gi:7657460. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. An in-frame AUG is located 12 codons upstream of the annotated translation start site but is not being annotated as a start site since it is poorly conserved and is in a weak Kozak sequence context. ##Evidence-Data-START## Transcript exon combination :: AF189279.1, BC069116.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

1
61
121
181
241
301
361
421
ATGAAATCTC CCCACGTGCT GGTGTTCCTT TGCCTCCTGG TGGCTCTGGT CACCGGGAAC 
CTGGTTCAGT TTGGGGTGAT GATCGAGAAG ATGACAGGCA AGTCCGCCCT GCAGTACAAC
GACTATGGCT GTTACTGCGG CATCGGTGGC TCCCACTGGC CGGTGGACCA GACTGACTGG
TGCTGCCACG CCCACGACTG CTGCTACGGG CGTCTGGAGA AGCTGGGCTG TGAGCCCAAA
CTGGAAAAGT ATCTTTTCTC TGTCAGCGAA CGTGGCATTT TCTGCGCCGG CAGGACCACC
TGCCAGCGGC TGACCTGCGA GTGTGACAAG AGGGCTGCCC TCTGCTTTCG CCGCAACCTG
GGCACCTACA ACCGCAAATA TGCCCATTAT CCCAACAAGC TGTGCACCGG GCCCACCCCG
CCCTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_055404.1
CDS59..487
Misc Feature(1)116..463(+)
Misc Feature(2)119..298(+)
Misc Feature(3)191..256(+)
Misc Feature(4)197..385(+)
Exon (1)1..98
Gene:PLA2G2E
Gene Synonym:
Exon (2)99..237
Gene:PLA2G2E
Gene Synonym:
Exon (3)238..344
Gene:PLA2G2E
Gene Synonym:
Exon (4)345..487
Gene:PLA2G2E
Gene Synonym:
Translation
Position Chain Variation Link
13 13 c, g dbSNP:769870817
28 28 c, t dbSNP:762121036
36 36 a, t dbSNP:544961785
49 49 a, c, g dbSNP:61753361
50 50 c, g, t dbSNP:368508285
53 53 a, c dbSNP:565307904
55 55 a, t dbSNP:540714785
73 73 c, t dbSNP:41264125
74 74 a, g dbSNP:756809037
75 75 g, t dbSNP:753540548
101 101 a, g dbSNP:369817622
112 112 c, t dbSNP:146848577
113 113 a, g dbSNP:369084711
115 115 c, g dbSNP:765732453
129 129 a, t dbSNP:757630456
132 132 c, g dbSNP:754249570
142 142 c, t dbSNP:764270158
143 143 a, c, g dbSNP:775498456
144 144 a, g dbSNP:140234785
145 145 g, t dbSNP:759322747
150 150 c, g, t dbSNP:201542503
153 153 c, g dbSNP:770828466
163 163 c, t dbSNP:749064942
164 164 a, g dbSNP:773178642
166 166 a, c dbSNP:769526597
167 167 c, t dbSNP:747821284
169 169 c, g dbSNP:148618051
170 170 c, t dbSNP:780504394
175 175 c, t dbSNP:754509470
178 178 a, c, t dbSNP:145303802
179 179 a, g dbSNP:757802583
180 180 a, c dbSNP:754163367
186 186 a, g dbSNP:572793290
190 190 g, t dbSNP:756270547
196 196 c, t dbSNP:752919581
197 197 a, g dbSNP:139719874
216 216 c, g dbSNP:551211892
217 217 -, g dbSNP:751228370
219 219 c, g dbSNP:774338921
220 220 a, g, t dbSNP:12731919
221 221 c, g dbSNP:773088642
236 236 g, t dbSNP:769718316
243 243 a, g dbSNP:775005614
247 247 c, t dbSNP:369294108
251 251 c, t dbSNP:535636403
254 254 a, g dbSNP:778239539
256 256 c, t dbSNP:770331034
257 257 a, t dbSNP:140744541
264 264 a, g dbSNP:781437830
265 265 c, t dbSNP:372491665
266 266 a, g dbSNP:751738554
269 269 c, t dbSNP:780494033
270 270 a, g dbSNP:61729971
286 286 c, t dbSNP:370199149
288 288 a, g dbSNP:150118869
292 292 a, g dbSNP:773902416
300 300 c, t dbSNP:533011527
306 306 a, c dbSNP:763808244
311 311 a, c dbSNP:760306746
320 320 a, g dbSNP:775119539
322 322 a, c dbSNP:771769821
326 326 a, g dbSNP:367824617
327 327 a, t dbSNP:778839822
329 329 a, c, g, t dbSNP:559232898
334 334 c, t dbSNP:201254783
340 340 c, t dbSNP:145027583
343 343 a, c, t dbSNP:374893489
346 346 a, c dbSNP:769258102
347 347 a, g dbSNP:747306185
366 366 a, g dbSNP:147846234
376 376 c, t dbSNP:374363002
377 377 a, g dbSNP:746108367
378 378 a, g dbSNP:767870884
389 389 a, c dbSNP:201523720
398 398 c, t dbSNP:749442824
410 410 c, t dbSNP:777548405
411 411 a, g dbSNP:61729970
420 420 a, g dbSNP:752445369
421 421 a, c, t dbSNP:149589390
429 429 a, g dbSNP:754564609
431 431 a, c, t dbSNP:766115475
432 432 a, g dbSNP:762351431
435 435 a, g dbSNP:78889206
436 436 a, g dbSNP:751232198
440 440 g, t dbSNP:761286861
442 442 c, g dbSNP:145173161
455 455 a, g dbSNP:772571269
460 460 a, g dbSNP:746301052
464 464 a, g dbSNP:774420800
465 465 c, g, t dbSNP:749295415
467 467 a, g dbSNP:777936322
477 477 a, c, t dbSNP:141984318
485 485 c, t dbSNP:781176341
486 486 a, g dbSNP:754756791

Target ORF information:

RefSeq Version NM_014589
Organism Homo sapiens (human)
Definition Homo sapiens phospholipase A2, group IIE (PLA2G2E), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_014589

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGAAATCTC CCCACGTGCT GGTGTTCCTT TGCCTCCTGG TGGCTCTGGT CACCGGGAAC 
CTGGTTCAGT TTGGGGTGAT GATCGAGAAG ATGACAGGCA AGTCCGCCCT GCAGTACAAC
GACTATGGCT GTTACTGCGG CATCGGTGGC TCCCACTGGC CGGTGGACCA GACTGACTGG
TGCTGCCACG CCCACGACTG CTGCTACGGG CGTCTGGAGA AGCTGGGCTG TGAGCCCAAA
CTGGAAAAGT ATCTTTTCTC TGTCAGCGAA CGTGGCATTT TCTGCGCCGG CAGGACCACC
TGCCAGCGGC TGACCTGCGA GTGTGACAAG AGGGCTGCCC TCTGCTTTCG CCGCAACCTG
GGCACCTACA ACCGCAAATA TGCCCATTAT CCCAACAAGC TGTGCACCGG GCCCACCCCG
CCCTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Genome-wide association study of ulcerative colitis in Koreans suggests extensive overlapping of genetic susceptibility with Caucasians
Inflamm. Bowel Dis. 19 (5), 954-966 (2013)
Yang SK, Hong M, Zhao W, Jung Y, Tayebi N, Ye BD, Kim KJ, Park SH, Lee I, Shin HD, Cheong HS, Kim LH, Kim HJ, Jung SA, Kang D, Youn HS, Liu J and Song K.


book

Genome-wide association identifies multiple ulcerative colitis susceptibility loci
Nat. Genet. 42 (4), 332-337 (2010)
McGovern DP, Gardet A, Torkvist L, Goyette P, Essers J, Taylor KD, Neale BM, Ong RT, Lagace C, Li C, Green T, Stevens CR, Beauchamp C, Fleshner PR, Carlson M, D'Amato M, Halfvarson J, Hibberd ML, Lordal M, Padyukov L, Andriulli A, Colombo E, Latiano A, Palmieri O, Bernard EJ, Deslandres C, Hommes DW, de Jong DJ, Stokkers PC, Weersma RK, Sharma Y, Silverberg MS, Cho JH, Wu J, Roeder K, Brant SR, Schumm LP, Duerr RH, Dubinsky MC, Glazer NL, Haritunians T, Ippoliti A, Melmed GY, Siscovick DS, Vasiliauskas EA, Targan SR, Annese V, Wijmenga C, Pettersson S, Rotter JI, Xavier RJ, Daly MJ, Rioux JD and Seielstad M.


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Polymorphisms in innate immunity genes and patients response to dendritic cell-based HIV immuno-treatment
Vaccine 28 (10), 2201-2206 (2010)
Segat L, Brandao LA, Guimaraes RL, Pontillo A, Athanasakis E, de Oliveira RM, Arraes LC, de Lima Filho JL and Crovella S.


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Genome-wide association study of ulcerative colitis identifies three new susceptibility loci, including the HNF4A region
Nat. Genet. 41 (12), 1330-1334 (2009)
Barrett JC, Lee JC, Lees CW, Prescott NJ, Anderson CA, Phillips A, Wesley E, Parnell K, Zhang H, Drummond H, Nimmo ER, Massey D, Blaszczyk K, Elliott T, Cotterill L, Dallal H, Lobo AJ, Mowat C, Sanderson JD, Jewell DP, Newman WG, Edwards C, Ahmad T, Mansfield JC, Satsangi J, Parkes M, Mathew CG, Donnelly P, Peltonen L, Blackwell JM, Bramon E, Brown MA, Casas JP, Corvin A, Craddock N, Deloukas P, Duncanson A, Jankowski J, Markus HS, Mathew CG, McCarthy MI, Palmer CN, Plomin R, Rautanen A, Sawcer SJ, Samani N, Trembath RC, Viswanathan AC, Wood N, Spencer CC, Barrett JC, Bellenguez C, Davison D, Freeman C, Strange A, Donnelly P, Langford C, Hunt SE, Edkins S, Gwilliam R, Blackburn H, Bumpstead SJ, Dronov S, Gillman M, Gray E, Hammond N, Jayakumar A, McCann OT, Liddle J, Perez ML, Potter SC, Ravindrarajah R, Ricketts M, Waller M, Weston P, Widaa S, Whittaker P, Deloukas P, Peltonen L, Mathew CG, Blackwell JM, Brown MA, Corvin A, McCarthy MI, Spencer CC, Attwood AP, Stephens J, Sambrook J, Ouwehand WH, McArdle WL, Ring SM and Strachan DP.


book

Ulcerative colitis-risk loci on chromosomes 1p36 and 12q15 found by genome-wide association study
Nat. Genet. 41 (2), 216-220 (2009)
Silverberg MS, Cho JH, Rioux JD, McGovern DP, Wu J, Annese V, Achkar JP, Goyette P, Scott R, Xu W, Barmada MM, Klei L, Daly MJ, Abraham C, Bayless TM, Bossa F, Griffiths AM, Ippoliti AF, Lahaie RG, Latiano A, Pare P, Proctor DD, Regueiro MD, Steinhart AH, Targan SR, Schumm LP, Kistner EO, Lee AT, Gregersen PK, Rotter JI, Brant SR, Taylor KD, Roeder K and Duerr RH.


book

Arachidonate release and eicosanoid generation by group IIE phospholipase A(2)
Biochem. Biophys. Res. Commun. 292 (3), 689-696 (2002)
Murakami M, Yoshihara K, Shimbara S, Lambeau G, Singer A, Gelb MH, Sawada M, Inagaki N, Nagai H and Kudo I.


book

Structures, enzymatic properties, and expression of novel human and mouse secretory phospholipase A(2)s
J. Biol. Chem. 275 (8), 5785-5793 (2000)
Suzuki N, Ishizaki J, Yokota Y, Higashino K, Ono T, Ikeda M, Fujii N, Kawamoto K and Hanasaki K.


 
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