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PLA2G2E cDNA ORF clone, Homo sapiens (human)

Gene Symbol PLA2G2E
Entrez Gene ID 30814
Full Name phospholipase A2, group IIE
Synonyms GIIE sPLA2, sPLA2-IIE
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1p36.13

Summary lac of sum

mRNA and Protein(s)

mRNA Protein Name
NM_014589 NP_055404 group IIE secretory phospholipase A2 precursor



Homo sapiens (human) PLA2G2E NP_055404.1
Pan troglodytes (chimpanzee) PLA2G2E XP_001163677.1
Macaca mulatta (Rhesus monkey) PLA2G2E XP_001094364.1
Canis lupus familiaris (dog) PLA2G2E XP_544525.3
Bos taurus (cattle) PLA2G2E NP_001179015.1
Mus musculus (house mouse) Pla2g2e NP_036174.1
Gallus gallus (chicken) PLA2G2E NP_001171878.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC100496855 XP_002938654.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC101735122 XP_004920461.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PLA2G2E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLA2G2E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu01793
NM_014589 Homo sapiens phospholipase A2, group IIE (PLA2G2E), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu01793
Clone ID Related Accession (Same CDS sequence) NM_014589
Accession Version NM_014589.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 429bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 20-FEB-2014
Organism Homo sapiens (human)
Product group IIE secretory phospholipase A2 precursor
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF189279.1 and AL358253.16. On Nov 17, 2012 this sequence version replaced gi:7657460. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. An in-frame AUG is located 12 codons upstream of the annotated translation start site but is not being annotated as a start site since it is poorly conserved and is in a weak Kozak sequence context. ##Evidence-Data-START## Transcript exon combination :: AF189279.1, BC069116.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

1
61
121
181
241
301
361
421
ATGAAATCTC CCCACGTGCT GGTGTTCCTT TGCCTCCTGG TGGCTCTGGT CACCGGGAAC 
CTGGTTCAGT TTGGGGTGAT GATCGAGAAG ATGACAGGCA AGTCCGCCCT GCAGTACAAC
GACTATGGCT GTTACTGCGG CATCGGTGGC TCCCACTGGC CGGTGGACCA GACTGACTGG
TGCTGCCACG CCCACGACTG CTGCTACGGG CGTCTGGAGA AGCTGGGCTG TGAGCCCAAA
CTGGAAAAGT ATCTTTTCTC TGTCAGCGAA CGTGGCATTT TCTGCGCCGG CAGGACCACC
TGCCAGCGGC TGACCTGCGA GTGTGACAAG AGGGCTGCCC TCTGCTTTCG CCGCAACCTG
GGCACCTACA ACCGCAAATA TGCCCATTAT CCCAACAAGC TGTGCACCGG GCCCACCCCG
CCCTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_055404.1
CDS59..487
Misc Feature(1)116..463(+)
Misc Feature(2)119..298(+)
Misc Feature(3)191..256(+)
Misc Feature(4)197..385(+)
Exon (1)1..98
Gene:PLA2G2E
Gene Synonym:
Exon (2)99..237
Gene:PLA2G2E
Gene Synonym:
Exon (3)238..344
Gene:PLA2G2E
Gene Synonym:
Exon (4)345..487
Gene:PLA2G2E
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_014589
Organism Homo sapiens (human)
Definition Homo sapiens phospholipase A2, group IIE (PLA2G2E), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_014589

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGAAATCTC CCCACGTGCT GGTGTTCCTT TGCCTCCTGG TGGCTCTGGT CACCGGGAAC 
CTGGTTCAGT TTGGGGTGAT GATCGAGAAG ATGACAGGCA AGTCCGCCCT GCAGTACAAC
GACTATGGCT GTTACTGCGG CATCGGTGGC TCCCACTGGC CGGTGGACCA GACTGACTGG
TGCTGCCACG CCCACGACTG CTGCTACGGG CGTCTGGAGA AGCTGGGCTG TGAGCCCAAA
CTGGAAAAGT ATCTTTTCTC TGTCAGCGAA CGTGGCATTT TCTGCGCCGG CAGGACCACC
TGCCAGCGGC TGACCTGCGA GTGTGACAAG AGGGCTGCCC TCTGCTTTCG CCGCAACCTG
GGCACCTACA ACCGCAAATA TGCCCATTAT CCCAACAAGC TGTGCACCGG GCCCACCCCG
CCCTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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Genome-wide association study of ulcerative colitis in Koreans suggests extensive overlapping of genetic susceptibility with Caucasians
Inflamm. Bowel Dis. 19 (5), 954-966 (2013)
Yang SK, Hong M, Zhao W, Jung Y, Tayebi N, Ye BD, Kim KJ, Park SH, Lee I, Shin HD, Cheong HS, Kim LH, Kim HJ, Jung SA, Kang D, Youn HS, Liu J and Song K.


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Genome-wide association identifies multiple ulcerative colitis susceptibility loci
Nat. Genet. 42 (4), 332-337 (2010)
McGovern DP, Gardet A, Torkvist L, Goyette P, Essers J, Taylor KD, Neale BM, Ong RT, Lagace C, Li C, Green T, Stevens CR, Beauchamp C, Fleshner PR, Carlson M, D'Amato M, Halfvarson J, Hibberd ML, Lordal M, Padyukov L, Andriulli A, Colombo E, Latiano A, Palmieri O, Bernard EJ, Deslandres C, Hommes DW, de Jong DJ, Stokkers PC, Weersma RK, Sharma Y, Silverberg MS, Cho JH, Wu J, Roeder K, Brant SR, Schumm LP, Duerr RH, Dubinsky MC, Glazer NL, Haritunians T, Ippoliti A, Melmed GY, Siscovick DS, Vasiliauskas EA, Targan SR, Annese V, Wijmenga C, Pettersson S, Rotter JI, Xavier RJ, Daly MJ, Rioux JD and Seielstad M.


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Polymorphisms in innate immunity genes and patients response to dendritic cell-based HIV immuno-treatment
Vaccine 28 (10), 2201-2206 (2010)
Segat L, Brandao LA, Guimaraes RL, Pontillo A, Athanasakis E, de Oliveira RM, Arraes LC, de Lima Filho JL and Crovella S.


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Genome-wide association study of ulcerative colitis identifies three new susceptibility loci, including the HNF4A region
Nat. Genet. 41 (12), 1330-1334 (2009)
Barrett JC, Lee JC, Lees CW, Prescott NJ, Anderson CA, Phillips A, Wesley E, Parnell K, Zhang H, Drummond H, Nimmo ER, Massey D, Blaszczyk K, Elliott T, Cotterill L, Dallal H, Lobo AJ, Mowat C, Sanderson JD, Jewell DP, Newman WG, Edwards C, Ahmad T, Mansfield JC, Satsangi J, Parkes M, Mathew CG, Donnelly P, Peltonen L, Blackwell JM, Bramon E, Brown MA, Casas JP, Corvin A, Craddock N, Deloukas P, Duncanson A, Jankowski J, Markus HS, Mathew CG, McCarthy MI, Palmer CN, Plomin R, Rautanen A, Sawcer SJ, Samani N, Trembath RC, Viswanathan AC, Wood N, Spencer CC, Barrett JC, Bellenguez C, Davison D, Freeman C, Strange A, Donnelly P, Langford C, Hunt SE, Edkins S, Gwilliam R, Blackburn H, Bumpstead SJ, Dronov S, Gillman M, Gray E, Hammond N, Jayakumar A, McCann OT, Liddle J, Perez ML, Potter SC, Ravindrarajah R, Ricketts M, Waller M, Weston P, Widaa S, Whittaker P, Deloukas P, Peltonen L, Mathew CG, Blackwell JM, Brown MA, Corvin A, McCarthy MI, Spencer CC, Attwood AP, Stephens J, Sambrook J, Ouwehand WH, McArdle WL, Ring SM and Strachan DP.


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Ulcerative colitis-risk loci on chromosomes 1p36 and 12q15 found by genome-wide association study
Nat. Genet. 41 (2), 216-220 (2009)
Silverberg MS, Cho JH, Rioux JD, McGovern DP, Wu J, Annese V, Achkar JP, Goyette P, Scott R, Xu W, Barmada MM, Klei L, Daly MJ, Abraham C, Bayless TM, Bossa F, Griffiths AM, Ippoliti AF, Lahaie RG, Latiano A, Pare P, Proctor DD, Regueiro MD, Steinhart AH, Targan SR, Schumm LP, Kistner EO, Lee AT, Gregersen PK, Rotter JI, Brant SR, Taylor KD, Roeder K and Duerr RH.


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Arachidonate release and eicosanoid generation by group IIE phospholipase A(2)
Biochem. Biophys. Res. Commun. 292 (3), 689-696 (2002)
Murakami M, Yoshihara K, Shimbara S, Lambeau G, Singer A, Gelb MH, Sawada M, Inagaki N, Nagai H and Kudo I.


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Structures, enzymatic properties, and expression of novel human and mouse secretory phospholipase A(2)s
J. Biol. Chem. 275 (8), 5785-5793 (2000)
Suzuki N, Ishizaki J, Yokota Y, Higashino K, Ono T, Ikeda M, Fujii N, Kawamoto K and Hanasaki K.


 
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