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HGF hepatocyte growth factor (hepapoietin A; scatter factor) [Homo sapiens (human)]

Gene Symbol HGF
Entrez Gene ID 3082
Full Name hepatocyte growth factor (hepapoietin A; scatter factor)
Synonyms DFNB39, F-TCF, HGFB, HPTA, SF
General protein information
Preferred Names
hepatocyte growth factor
Names
hepatocyte growth factor
hepatopoeitin-A
hepatopoietin-A
lung fibroblast-derived mitogen
fibroblast-derived tumor cytotoxic factor
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7q21.1

Summary Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

142409

Disorder Html: Deafness, autosomal recessive 39, 608265 (3)
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu25775 XM_006715956 PREDICTED: Homo sapiens hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu25877 XM_011516115 PREDICTED: Homo sapiens hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 12-14 Starting from $99.00
OHu25798 NM_001010931 Homo sapiens hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu26016 NM_001010933 Homo sapiens hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu25927 NM_001010934 Homo sapiens hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu25775 NM_000601 Homo sapiens hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu25877 NM_001010932 Homo sapiens hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK Not in stock 12-14 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu25775D
Sequence Information ORF Nucleotide Sequence (Length: 2187bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product hepatocyte growth factor isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578814178. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA GAGTTATCGA
GGTCTCATGG ATCATACAGA ATCAGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCA
CACCGGCACA AATTCTTGCC TGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC
CGCAATCCCG ATGGCCAGCC GAGGCCATGG TGCTATACTC TTGACCCTCA CACCCGCTGG
GAGTACTGTG CAATTAAAAC ATGCGCTGAC AATACTATGA ATGACACTGA TGTTCCTTTG
GAAACAACTG AATGCATCCA AGGTCAAGGA GAAGGCTACA GGGGCACTGT CAATACCATT
TGGAATGGAA TTCCATGTCA GCGTTGGGAT TCTCAGTATC CTCACGAGCA TGACATGACT
CCTGAAAATT TCAAGTGCAA GGACCTACGA GAAAATTACT GCCGAAATCC AGATGGGTCT
GAATCACCCT GGTGTTTTAC CACTGATCCA AACATCCGAG TTGGCTACTG CTCCCAAATT
CCAAACTGTG ATATGTCACA TGGACAAGAT TGTTATCGTG GGAATGGCAA AAATTATATG
GGCAACTTAT CCCAAACAAG ATCTGGACTA ACATGTTCAA TGTGGGACAA GAACATGGAA
GACTTACATC GTCATATCTT CTGGGAACCA GATGCAAGTA AGCTGAATGA GAATTACTGC
CGAAATCCAG ATGATGATGC TCATGGACCC TGGTGCTACA CGGGAAATCC ACTCATTCCT
TGGGATTATT GCCCTATTTC TCGTTGTGAA GGTGATACCA CACCTACAAT AGTCAATTTA
GACCATCCCG TAATATCTTG TGCCAAAACG AAACAATTGC GAGTTGTAAA TGGGATTCCA
ACACGAACAA ACATAGGATG GATGGTTAGT TTGAGATACA GAAATAAACA TATCTGCGGA
GGATCATTGA TAAAGGAGAG TTGGGTTCTT ACTGCACGAC AGTGTTTCCC TTCTCGAGAC
TTGAAAGATT ATGAAGCTTG GCTTGGAATT CATGATGTCC ACGGAAGAGG AGATGAGAAA
TGCAAACAGG TTCTCAATGT TTCCCAGCTG GTATATGGCC CTGAAGGATC AGATCTGGTT
TTAATGAAGC TTGCCAGGCC TGCTGTCCTG GATGATTTTG TTAGTACGAT TGATTTACCT
AATTATGGAT GCACAATTCC TGAAAAGACC AGTTGCAGTG TTTATGGCTG GGGCTACACT
GGATTGATCA ACTATGATGG CCTATTACGA GTGGCACATC TCTATATAAT GGGAAATGAG
AAATGCAGCC AGCATCATCG AGGGAAGGTG ACTCTGAATG AGTCTGAAAT ATGTGCTGGG
GCTGAAAAGA TTGGATCAGG ACCATGTGAG GGGGATTATG GTGGCCCACT TGTTTGTGAG
CAACATAAAA TGAGAATGGT TCTTGGTGTC ATTGTTCCTG GTCGTGGATG TGCCATTCCA
AATCGTCCTG GTATTTTTGT CCGAGTAGCA TATTATGCAA AATGGATACA CAAAATTATT
TTAACATATA AGGTACCACA GTCATAG
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_006716019.1
CDS191..2377
Misc Feature(1)305..556(+)
Misc Feature(2)368..409(+)
Misc Feature(3)566..811(+)
Misc Feature(4)599..601(+)
Misc Feature(5)644..784(+)
Misc Feature(6)812..1057(+)
Misc Feature(7)848..850(+)
Misc Feature(8)893..1030(+)
Misc Feature(9)1094..1342(+)
Misc Feature(10)1130..1132(+)
Misc Feature(11)1175..1312(+)
Misc Feature(12)1352..1600(+)
Misc Feature(13)1388..1390(+)
Misc Feature(14)1433..1573(+)
Misc Feature(15)1670..2338(+)
Misc Feature(16)1673..2347(+)
Misc Feature(17)1673..1675(+)
Misc Feature(18)1790..2209(+)
Misc Feature(19)2189..2272(+)
Translation
Position Chain Variation Link
71 71 a, t dbSNP:5745606
130 130 a, g dbSNP:759006519
150 150 a, c dbSNP:768292342
151 151 a, t dbSNP:28719812
174 174 g, t dbSNP:779801717
176 176 c, t dbSNP:370842395
177 177 c, g dbSNP:201598927
194 194 c, t dbSNP:375901177
196 196 g, t dbSNP:745851853
198 198 a, t dbSNP:778667611
217 217 c, t dbSNP:372751973
238 238 c, t dbSNP:148935472
242 242 c, t dbSNP:752758599
247 247 c, t dbSNP:781631751
248 248 a, c dbSNP:755241254
252 252 c, t dbSNP:370490787
263 263 -, gccatc dbSNP:764462723
282 282 a, g dbSNP:747253986
294 294 a, g, t dbSNP:758790721
295 295 a, g dbSNP:750744461
296 296 a, g dbSNP:765830204
297 297 -, gaa dbSNP:771230803
311 311 a, g dbSNP:376573289
318 318 a, c, g dbSNP:763623534
319 319 a, g dbSNP:17855203
323 323 a, t dbSNP:760130608
327 327 c, t dbSNP:150267054
336 336 a, c dbSNP:368094683
339 339 c, t dbSNP:759442724
346 346 a, g dbSNP:774007780
348 348 c, t dbSNP:746355265
364 364 a, g dbSNP:749210341
372 372 a, c, t dbSNP:142659098
373 373 c, t dbSNP:147718886
389 389 a, g dbSNP:780386537
406 406 c, t dbSNP:372588506
409 409 a, t dbSNP:746293406
419 419 a, g dbSNP:765051974
428 428 c, t dbSNP:757576097
433 433 a, g dbSNP:571028390
436 436 c, g dbSNP:778196890
448 448 c, t dbSNP:370202240
460 460 c, t dbSNP:145598174
462 462 a, g dbSNP:771300057
468 468 c, g dbSNP:189052337
475 475 a, g dbSNP:778319588
487 487 c, t dbSNP:368633795
496 496 c, t dbSNP:752305781
500 500 a, g dbSNP:780541101
522 522 a, t dbSNP:754474625
523 523 a, g dbSNP:5745635
525 525 g, t dbSNP:766059183
537 537 a, t dbSNP:151170133
544 544 c, t dbSNP:769553578
546 546 a, t dbSNP:750225776
562 562 c, t dbSNP:537365064
563 563 a, g dbSNP:781690781
577 577 a, c dbSNP:754448554
579 579 c, t dbSNP:141389863
582 582 a, g dbSNP:779906703
585 585 a, g dbSNP:758097772
591 591 a, g dbSNP:750282888
601 601 a, g dbSNP:765155428
610 610 a, g dbSNP:757026543
634 634 -, a dbSNP:771780436
636 636 a, g dbSNP:753731630
640 640 a, g dbSNP:764026915
648 648 g, t dbSNP:17566
661 661 a, g dbSNP:142045938
664 664 c, t dbSNP:367553438
673 673 c, t dbSNP:762973783
684 684 c, t dbSNP:370760302
685 685 a, g dbSNP:137853235
687 687 a, g dbSNP:373660832
690 690 a, g dbSNP:762140462
692 692 c, t dbSNP:777026877
693 693 a, g dbSNP:773949476
697 697 c, t dbSNP:540789414
714 714 a, g dbSNP:73381183
716 716 c, t dbSNP:747549889
723 723 a, g dbSNP:776090604
731 731 a, c dbSNP:771720187
746 746 a, g dbSNP:745308839
747 747 a, g dbSNP:778497868
768 768 a, g dbSNP:148615168
770 770 c, g dbSNP:749085781
779 779 c, t dbSNP:777593933
780 780 a, g dbSNP:558400297
784 784 c, t dbSNP:143891160
794 794 a, g dbSNP:781053176
810 810 c, t dbSNP:377663087
828 828 c, g dbSNP:144136982
849 849 a, g dbSNP:372600489
872 872 g, t dbSNP:139457161
889 889 a, g dbSNP:754102922
901 901 c, t dbSNP:5745666
905 905 a, g dbSNP:761186765
907 907 a, g dbSNP:753099990
909 909 c, t dbSNP:768190212
911 911 c, t dbSNP:760088738
914 914 c, t dbSNP:202185530
915 915 a, g dbSNP:200559590
919 919 c, t dbSNP:762316469
928 928 a, g dbSNP:573737350
931 931 c, t dbSNP:772926410
943 943 c, t dbSNP:755250749
944 944 a, g dbSNP:752123971
948 948 a, g dbSNP:559265540
951 951 c, g, t dbSNP:560215969
952 952 c, t dbSNP:772684432
963 963 a, c dbSNP:764905780
965 965 c, t dbSNP:761481749
967 967 c, t dbSNP:776214617
970 970 c, t dbSNP:375177081
972 972 a, g dbSNP:768451705
979 979 c, t dbSNP:147312555
984 984 c, g dbSNP:775564237
990 990 c, t dbSNP:771889279
992 992 a, g dbSNP:749434892
1021 1021 a, c dbSNP:777970277
1022 1022 a, t dbSNP:754578668
1025 1025 c, t dbSNP:756224070
1026 1026 g, t dbSNP:371502313
1039 1039 g, t dbSNP:533086523
1040 1040 a, g dbSNP:755376784
1044 1044 c, t dbSNP:751870029
1050 1050 c, t dbSNP:766971992
1051 1051 a, g dbSNP:368002816
1054 1054 c, t dbSNP:374136304
1055 1055 a, g dbSNP:764746019
1060 1060 c, t dbSNP:778286006
1062 1062 a, c dbSNP:755584740
1067 1067 a, g dbSNP:747693036
1068 1068 a, t dbSNP:780768372
1070 1070 a, t dbSNP:754659494
1073 1073 c, g dbSNP:367811213
1075 1075 a, c, t dbSNP:141751290
1078 1078 a, t dbSNP:750329013
1083 1083 g, t dbSNP:775912518
1086 1086 c, t dbSNP:760721822
1095 1095 c, t dbSNP:770152785
1100 1100 a, g dbSNP:5745687
1107 1107 a, t dbSNP:767766226
1110 1110 a, c dbSNP:759784966
1111 1111 a, g dbSNP:774508303
1122 1122 a, g dbSNP:771299734
1124 1124 a, g dbSNP:749600508
1126 1126 c, t dbSNP:773660386
1130 1130 a, t dbSNP:781437504
1138 1138 g, t dbSNP:770323794
1147 1147 c, t dbSNP:747664002
1150 1150 c, t dbSNP:780821031
1172 1172 c, t dbSNP:754502188
1173 1173 a, g, t dbSNP:374484762
1174 1174 c, t dbSNP:779783865
1178 1178 g, t dbSNP:5745688
1179 1179 a, t dbSNP:750217931
1189 1189 c, t dbSNP:569559437
1195 1195 c, t dbSNP:757186641
1196 1196 a, g dbSNP:549654942
1198 1198 a, g dbSNP:148714837
1219 1219 c, t dbSNP:759555986
1225 1225 a, g dbSNP:751786738
1227 1227 a, g dbSNP:766585116
1233 1233 a, g dbSNP:763206701
1238 1238 a, c dbSNP:373442319
1239 1239 a, g dbSNP:750575553
1240 1240 a, g dbSNP:547103298
1263 1263 a, g dbSNP:184858341
1270 1270 c, t dbSNP:765660542
1273 1273 a, t dbSNP:762012189
1281 1281 a, g dbSNP:5745703
1288 1288 c, t dbSNP:777341007
1304 1304 a, t dbSNP:769270957
1318 1318 c, t dbSNP:112557787
1325 1325 a, c dbSNP:200948659
1328 1328 a, g dbSNP:771441595
1340 1340 a, g dbSNP:745555932
1341 1341 a, g dbSNP:778372935
1343 1343 a, g dbSNP:770765351
1349 1349 a, c dbSNP:749078919
1350 1350 a, t dbSNP:777748021
1367 1367 c, t dbSNP:758567520
1368 1368 a, g dbSNP:745919057
1369 1369 c, t dbSNP:377036882
1377 1377 c, g dbSNP:745854997
1384 1384 c, t dbSNP:754071169
1391 1391 a, g dbSNP:764574926
1393 1393 c, t dbSNP:756489380
1420 1420 a, g dbSNP:373298029
1452 1452 a, c dbSNP:767907135
1460 1460 c, t dbSNP:759145490
1465 1465 c, t dbSNP:755416276
1481 1481 c, g dbSNP:61735997
1484 1484 a, g dbSNP:752052736
1514 1514 a, c dbSNP:765902946
1532 1532 c, g dbSNP:762379250
1549 1549 a, c dbSNP:750085970
1551 1551 c, t dbSNP:764869064
1552 1552 a, g dbSNP:761711142
1556 1556 a, c dbSNP:776270506
1564 1564 c, t dbSNP:566128293
1570 1570 c, t dbSNP:760563094
1578 1578 a, g dbSNP:775433097
1586 1586 a, t dbSNP:141487191
1593 1593 a, g dbSNP:148293411
1612 1612 a, g dbSNP:764922029
1634 1634 c, t dbSNP:761396857
1639 1639 c, t dbSNP:375872396
1640 1640 a, g, t dbSNP:571102402
1642 1642 a, g dbSNP:759566751
1644 1644 c, t dbSNP:397516446
1646 1646 c, t dbSNP:774389172
1656 1656 a, g dbSNP:371520373
1660 1660 a, g, t dbSNP:372075114
1667 1667 c, t dbSNP:768896096
1694 1694 -, c dbSNP:748881407
1697 1697 a, g dbSNP:747426081
1698 1698 c, g dbSNP:775857995
1703 1703 a, g dbSNP:772388735
1711 1711 a, g dbSNP:200549396
1714 1714 g, t dbSNP:746380080
1718 1718 a, g dbSNP:779336001
1722 1722 c, t dbSNP:756776269
1727 1727 c, t dbSNP:572203404
1743 1743 c, t dbSNP:776754331
1753 1753 a, t dbSNP:764035851
1768 1768 c, g dbSNP:143262934
1771 1771 c, t dbSNP:775625219
1780 1780 a, t dbSNP:772441910
1784 1784 a, g dbSNP:759881207
1787 1787 c, t dbSNP:527951814
1788 1788 a, g, t dbSNP:771437724
1789 1789 a, g dbSNP:748796647
1804 1804 a, t dbSNP:368519009
1817 1817 g, t dbSNP:769438908
1839 1839 a, t dbSNP:192078086
1842 1842 a, c, g dbSNP:752861058
1852 1852 c, t dbSNP:148098479
1853 1853 a, g dbSNP:779933934
1862 1862 a, g dbSNP:370757602
1864 1864 c, g, t dbSNP:144086354
1865 1865 a, g dbSNP:778704152
1875 1875 a, g dbSNP:559990458
1877 1877 c, g dbSNP:376043671
1888 1888 c, t dbSNP:752743857
1894 1894 c, t dbSNP:138548003
1898 1898 c, t dbSNP:759776706
1903 1903 a, g dbSNP:751689885
1905 1905 a, g dbSNP:766769838
1915 1915 a, g dbSNP:763167149
1921 1921 a, g dbSNP:773824390
1935 1935 c, t dbSNP:765831517
1936 1936 a, g dbSNP:201395847
1954 1954 g, t dbSNP:531850775
1955 1955 a, g dbSNP:538415452
1963 1963 c, t dbSNP:199516810
1966 1966 c, t dbSNP:754944443
1977 1977 c, t dbSNP:751819844
1978 1978 a, g dbSNP:766493585
1983 1983 a, g dbSNP:758862083
2004 2004 c, t dbSNP:147075806
2006 2006 a, g dbSNP:765890500
2008 2008 c, t dbSNP:762445545
2019 2019 a, c dbSNP:777216192
2022 2022 c, g dbSNP:141774517
2028 2028 a, g dbSNP:760093179
2038 2038 c, g dbSNP:775281878
2043 2043 g, t dbSNP:771594786
2047 2047 c, t dbSNP:200374420
2049 2049 c, t dbSNP:774153258
2052 2052 c, g dbSNP:770672389
2056 2056 a, g dbSNP:376395424
2057 2057 a, g dbSNP:371826587
2059 2059 c, t dbSNP:755575139
2062 2062 a, c, t dbSNP:373545882
2070 2070 a, g dbSNP:770723689
2074 2074 a, g dbSNP:762721825
2075 2075 c, t dbSNP:773198515
2078 2078 c, t dbSNP:769585774
2079 2079 a, g dbSNP:747158538
2081 2081 a, g dbSNP:145494248
2088 2088 a, c, g dbSNP:746110664
2090 2090 a, c dbSNP:779267373
2094 2094 a, g dbSNP:565056780
2096 2096 a, g dbSNP:754226152
2107 2107 c, t dbSNP:778395987
2109 2109 a, g dbSNP:756602776
2124 2124 a, g dbSNP:202215700
2129 2129 c, t dbSNP:545249104
2132 2132 a, g dbSNP:150109563
2134 2134 a, g dbSNP:369418750
2144 2144 c, t dbSNP:751270979
2155 2155 c, t dbSNP:766007676
2169 2169 c, g, t dbSNP:772967560
2171 2171 a, g dbSNP:769580628
2180 2180 a, t dbSNP:761664295
2184 2184 g, t dbSNP:776576073
2197 2197 c, t dbSNP:772105726
2202 2202 a, g dbSNP:770114698
2209 2209 c, t dbSNP:367800414
2215 2215 c, t dbSNP:781586687
2218 2218 a, g dbSNP:755450918
2222 2222 c, g dbSNP:747463316
2228 2228 a, g dbSNP:544960907
2231 2231 c, g dbSNP:779568330
2249 2249 g, t dbSNP:757849670
2260 2260 a, c dbSNP:750104031
2272 2272 a, t dbSNP:563705580
2274 2274 a, g dbSNP:543827901
2285 2285 a, g dbSNP:372366408
2295 2295 a, g dbSNP:763933267
2300 2300 a, g dbSNP:760728215
2304 2304 g, t dbSNP:775503666
2312 2312 c, t dbSNP:201768315
2313 2313 a, g dbSNP:763072412
2320 2320 a, c, g dbSNP:267601578
2326 2326 c, t dbSNP:770025635
2329 2329 a, t dbSNP:764730119
2356 2356 a, g dbSNP:561484014
2359 2359 c, t dbSNP:777071855
2369 2369 c, t dbSNP:140790665
2373 2373 a, c dbSNP:747516423
2375 2375 c, t dbSNP:780528260
2377 2377 a, g dbSNP:757972650
2383 2383 a, g dbSNP:745356078
2385 2385 -, aagt dbSNP:779001317
2387 2387 a, g dbSNP:778483338
2399 2399 c, t dbSNP:756957205
2406 2406 a, c dbSNP:753392862
2408 2408 a, g dbSNP:777717109
2413 2413 a, g dbSNP:767332482
2417 2417 a, c, t dbSNP:377185441
2422 2422 c, t dbSNP:767556298
2425 2425 a, c, g dbSNP:750490813
2426 2426 c, t dbSNP:765177187
2434 2434 g, t dbSNP:542402530
2443 2443 a, g dbSNP:573406409
2479 2479 -, aact dbSNP:374789745
2545 2545 c, t dbSNP:531222230
2598 2598 a, g dbSNP:544879896
2601 2601 a, g dbSNP:539702697
2636 2636 a, c dbSNP:777313117
2643 2643 -, ca dbSNP:763357973
2643 2643 c, g dbSNP:577625800
2649 2649 a, t dbSNP:758013381
2679 2679 a, t dbSNP:186434043
2691 2691 -, taat dbSNP:545984462
2730 2730 a, g dbSNP:537468151
2764 2764 c, t dbSNP:568550366
2773 2773 a, t dbSNP:182057547
2791 2791 a, c dbSNP:535633453
2829 2829 c, g dbSNP:778665027
2840 2840 a, t dbSNP:566500466
2844 2844 a, g dbSNP:5745768
2869 2869 a, t dbSNP:533030741
2899 2899 c, g dbSNP:754673292
2900 2900 -, tt dbSNP:5745769
2903 2903 -, tt dbSNP:567921457
2931 2931 a, g dbSNP:759206756
2946 2946 a, t dbSNP:753671589
2965 2965 a, g dbSNP:766188562
2974 2974 c, t dbSNP:140696262
2975 2975 a, g dbSNP:756101495
2989 2989 c, g dbSNP:75716542
3033 3033 a, c dbSNP:5745770
3034 3034 a, c dbSNP:561820689
3071 3071 g, t dbSNP:750511884
3119 3119 a, c dbSNP:541515764
3127 3127 -, ctt dbSNP:777098170
3152 3152 c, t dbSNP:537478018
3185 3185 g, t dbSNP:574028648
3191 3191 c, g dbSNP:559655621
3196 3196 a, g dbSNP:776314803
3211 3211 a, t dbSNP:546438057
3310 3310 c, t dbSNP:577589184
3335 3335 g, t dbSNP:189832870
3351 3351 a, c dbSNP:185299752
3357 3357 a, g dbSNP:192611863
3386 3386 a, g dbSNP:5745772
3411 3411 a, g dbSNP:5745773
3466 3466 c, t dbSNP:184092464
3473 3473 a, t dbSNP:28677052
3479 3479 a, g dbSNP:775942537
3492 3492 a, g dbSNP:760241049
3537 3537 a, g dbSNP:566188748
3540 3540 a, c dbSNP:546456660
3544 3544 a, g dbSNP:578009350
3642 3642 a, c dbSNP:539282857
3647 3647 a, g dbSNP:5745774
3678 3678 -, t dbSNP:538206512
3679 3679 g, t dbSNP:772057333
3694 3694 c, t dbSNP:191265665
3760 3760 a, c dbSNP:542200179
3790 3790 c, t dbSNP:746412693
3799 3799 c, g dbSNP:530719432
3801 3801 a, g dbSNP:567925018
3894 3894 c, g dbSNP:4732402
3898 3898 a, g dbSNP:771628734
3905 3905 a, g dbSNP:5745775
3926 3926 a, g dbSNP:186791770
3937 3937 c, t dbSNP:559424345
3945 3945 a, c dbSNP:554838290
3968 3968 a, c dbSNP:555372511
3999 3999 c, t dbSNP:5745776
4032 4032 c, t dbSNP:5745777
4053 4053 c, g dbSNP:535744424
4055 4055 g, t dbSNP:5745778
4065 4065 a, g dbSNP:544081230
4093 4093 a, g dbSNP:768386941
4120 4120 c, t dbSNP:566864574
4156 4156 a, t dbSNP:575086470
4168 4168 g, t dbSNP:554639010
4181 4181 g, t dbSNP:10262924
4186 4186 -, tt dbSNP:373238903
4226 4226 c, t dbSNP:748988745
4262 4262 a, c dbSNP:183089338
4284 4284 a, g dbSNP:558667567
4287 4287 -, ctc dbSNP:67776924
4289 4289 -, cct dbSNP:5745779
4304 4304 a, g dbSNP:539248931
4341 4341 c, t dbSNP:570267972
4353 4353 c, t dbSNP:5745780
4409 4409 a, g dbSNP:5745781
4462 4462 c, g dbSNP:756092978
4546 4546 a, g dbSNP:73155686
4595 4595 c, t dbSNP:568363721
4596 4596 a, g dbSNP:144029351
4607 4607 c, t dbSNP:5745782
4631 4631 a, g dbSNP:193254527
4662 4662 g, t dbSNP:552347951
4702 4702 c, g dbSNP:750454785
4765 4765 g, t dbSNP:532246490
4824 4824 a, c dbSNP:547131850
4849 4849 c, t dbSNP:757357420
4851 4851 g, t dbSNP:563830772
4857 4857 a, c dbSNP:188253748
4886 4886 a, g dbSNP:530308510
4899 4899 a, g dbSNP:146217138
4950 4950 a, c dbSNP:764169368
4981 4981 a, g dbSNP:763282702
5099 5099 c, t dbSNP:571285366
5108 5108 a, g dbSNP:752971849
5121 5121 c, g dbSNP:142379914
5161 5161 -, a dbSNP:34075472
5167 5167 g, t dbSNP:572453182
5183 5183 a, t dbSNP:148783522
5208 5208 a, t dbSNP:545061143
5211 5211 a, c, t dbSNP:149351591
5254 5254 a, t dbSNP:569697536
5271 5271 a, g dbSNP:538570220
5300 5300 c, t dbSNP:569458327
5312 5312 g, t dbSNP:537276806
5342 5342 c, t dbSNP:531462048
5343 5343 a, t dbSNP:777064011
5372 5372 a, g dbSNP:781609608
5446 5446 -, a dbSNP:757549796
5481 5481 g, t dbSNP:183726882
5664 5664 a, t dbSNP:367964046
5779 5779 a, t dbSNP:5745783
5793 5793 a, t dbSNP:1743
5894 5894 -, gtt dbSNP:777658790
5918 5918 a, t dbSNP:565624689
5942 5942 a, t dbSNP:79911513
5945 5945 a, g dbSNP:5745784
5950 5950 a, g dbSNP:5745785

Target ORF information:

RefSeq Version XM_006715956
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_006715956

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA GAGTTATCGA
GGTCTCATGG ATCATACAGA ATCAGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCA
CACCGGCACA AATTCTTGCC TGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC
CGCAATCCCG ATGGCCAGCC GAGGCCATGG TGCTATACTC TTGACCCTCA CACCCGCTGG
GAGTACTGTG CAATTAAAAC ATGCGCTGAC AATACTATGA ATGACACTGA TGTTCCTTTG
GAAACAACTG AATGCATCCA AGGTCAAGGA GAAGGCTACA GGGGCACTGT CAATACCATT
TGGAATGGAA TTCCATGTCA GCGTTGGGAT TCTCAGTATC CTCACGAGCA TGACATGACT
CCTGAAAATT TCAAGTGCAA GGACCTACGA GAAAATTACT GCCGAAATCC AGATGGGTCT
GAATCACCCT GGTGTTTTAC CACTGATCCA AACATCCGAG TTGGCTACTG CTCCCAAATT
CCAAACTGTG ATATGTCACA TGGACAAGAT TGTTATCGTG GGAATGGCAA AAATTATATG
GGCAACTTAT CCCAAACAAG ATCTGGACTA ACATGTTCAA TGTGGGACAA GAACATGGAA
GACTTACATC GTCATATCTT CTGGGAACCA GATGCAAGTA AGCTGAATGA GAATTACTGC
CGAAATCCAG ATGATGATGC TCATGGACCC TGGTGCTACA CGGGAAATCC ACTCATTCCT
TGGGATTATT GCCCTATTTC TCGTTGTGAA GGTGATACCA CACCTACAAT AGTCAATTTA
GACCATCCCG TAATATCTTG TGCCAAAACG AAACAATTGC GAGTTGTAAA TGGGATTCCA
ACACGAACAA ACATAGGATG GATGGTTAGT TTGAGATACA GAAATAAACA TATCTGCGGA
GGATCATTGA TAAAGGAGAG TTGGGTTCTT ACTGCACGAC AGTGTTTCCC TTCTCGAGAC
TTGAAAGATT ATGAAGCTTG GCTTGGAATT CATGATGTCC ACGGAAGAGG AGATGAGAAA
TGCAAACAGG TTCTCAATGT TTCCCAGCTG GTATATGGCC CTGAAGGATC AGATCTGGTT
TTAATGAAGC TTGCCAGGCC TGCTGTCCTG GATGATTTTG TTAGTACGAT TGATTTACCT
AATTATGGAT GCACAATTCC TGAAAAGACC AGTTGCAGTG TTTATGGCTG GGGCTACACT
GGATTGATCA ACTATGATGG CCTATTACGA GTGGCACATC TCTATATAAT GGGAAATGAG
AAATGCAGCC AGCATCATCG AGGGAAGGTG ACTCTGAATG AGTCTGAAAT ATGTGCTGGG
GCTGAAAAGA TTGGATCAGG ACCATGTGAG GGGGATTATG GTGGCCCACT TGTTTGTGAG
CAACATAAAA TGAGAATGGT TCTTGGTGTC ATTGTTCCTG GTCGTGGATG TGCCATTCCA
AATCGTCCTG GTATTTTTGT CCGAGTAGCA TATTATGCAA AATGGATACA CAAAATTATT
TTAACATATA AGGTACCACA GTCATAG

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu25877D
Sequence Information ORF Nucleotide Sequence (Length: 2172bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product hepatocyte growth factor isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTATCGGG GTAAAGACCT ACAGGAAAAC TACTGTCGAA ATCCTCGAGG GGAAGAAGGG
GGACCCTGGT GTTTCACAAG CAATCCAGAG GTACGCTACG AAGTCTGTGA CATTCCTCAG
TGTTCAGAAG TTGAATGCAT GACCTGCAAT GGGGAGAGTT ATCGAGGTCT CATGGATCAT
ACAGAATCAG GCAAGATTTG TCAGCGCTGG GATCATCAGA CACCACACCG GCACAAATTC
TTGCCTGAAA GATATCCCGA CAAGGGCTTT GATGATAATT ATTGCCGCAA TCCCGATGGC
CAGCCGAGGC CATGGTGCTA TACTCTTGAC CCTCACACCC GCTGGGAGTA CTGTGCAATT
AAAACATGCG CTGACAATAC TATGAATGAC ACTGATGTTC CTTTGGAAAC AACTGAATGC
ATCCAAGGTC AAGGAGAAGG CTACAGGGGC ACTGTCAATA CCATTTGGAA TGGAATTCCA
TGTCAGCGTT GGGATTCTCA GTATCCTCAC GAGCATGACA TGACTCCTGA AAATTTCAAG
TGCAAGGACC TACGAGAAAA TTACTGCCGA AATCCAGATG GGTCTGAATC ACCCTGGTGT
TTTACCACTG ATCCAAACAT CCGAGTTGGC TACTGCTCCC AAATTCCAAA CTGTGATATG
TCACATGGAC AAGATTGTTA TCGTGGGAAT GGCAAAAATT ATATGGGCAA CTTATCCCAA
ACAAGATCTG GACTAACATG TTCAATGTGG GACAAGAACA TGGAAGACTT ACATCGTCAT
ATCTTCTGGG AACCAGATGC AAGTAAGCTG AATGAGAATT ACTGCCGAAA TCCAGATGAT
GATGCTCATG GACCCTGGTG CTACACGGGA AATCCACTCA TTCCTTGGGA TTATTGCCCT
ATTTCTCGTT GTGAAGGTGA TACCACACCT ACAATAGTCA ATTTAGACCA TCCCGTAATA
TCTTGTGCCA AAACGAAACA ATTGCGAGTT GTAAATGGGA TTCCAACACG AACAAACATA
GGATGGATGG TTAGTTTGAG ATACAGAAAT AAACATATCT GCGGAGGATC ATTGATAAAG
GAGAGTTGGG TTCTTACTGC ACGACAGTGT TTCCCTTCTC GAGACTTGAA AGATTATGAA
GCTTGGCTTG GAATTCATGA TGTCCACGGA AGAGGAGATG AGAAATGCAA ACAGGTTCTC
AATGTTTCCC AGCTGGTATA TGGCCCTGAA GGATCAGATC TGGTTTTAAT GAAGCTTGCC
AGGCCTGCTG TCCTGGATGA TTTTGTTAGT ACGATTGATT TACCTAATTA TGGATGCACA
ATTCCTGAAA AGACCAGTTG CAGTGTTTAT GGCTGGGGCT ACACTGGATT GATCAACTAT
GATGGCCTAT TACGAGTGGC ACATCTCTAT ATAATGGGAA ATGAGAAATG CAGCCAGCAT
CATCGAGGGA AGGTGACTCT GAATGAGTCT GAAATATGTG CTGGGGCTGA AAAGATTGGA
TCAGGACCAT GTGAGGGGGA TTATGGTGGC CCACTTGTTT GTGAGCAACA TAAAATGAGA
ATGGTTCTTG GTGTCATTGT TCCTGGTCGT GGATGTGCCA TTCCAAATCG TCCTGGTATT
TTTGTCCGAG TAGCATATTA TGCAAAATGG ATACACAAAA TTATTTTAAC ATATAAGGTA
CCACAGTCATT AG
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011514417.1
CDS191..2362
Misc Feature(1)305..556(+)
Misc Feature(2)368..409(+)
Misc Feature(3)566..796(+)
Misc Feature(4)599..601(+)
Misc Feature(5)644..769(+)
Misc Feature(6)797..1042(+)
Misc Feature(7)833..835(+)
Misc Feature(8)878..1015(+)
Misc Feature(9)1079..1327(+)
Misc Feature(10)1115..1117(+)
Misc Feature(11)1160..1297(+)
Misc Feature(12)1337..1585(+)
Misc Feature(13)1373..1375(+)
Misc Feature(14)1418..1558(+)
Misc Feature(15)1655..2323(+)
Misc Feature(16)1658..2332(+)
Misc Feature(17)1658..1660(+)
Misc Feature(18)1775..2194(+)
Misc Feature(19)2174..2257(+)
Translation
Position Chain Variation Link
71 71 a, t dbSNP:5745606
130 130 a, g dbSNP:759006519
150 150 a, c dbSNP:768292342
151 151 a, t dbSNP:28719812
174 174 g, t dbSNP:779801717
176 176 c, t dbSNP:370842395
177 177 c, g dbSNP:201598927
194 194 c, t dbSNP:375901177
196 196 g, t dbSNP:745851853
198 198 a, t dbSNP:778667611
217 217 c, t dbSNP:372751973
238 238 c, t dbSNP:148935472
242 242 c, t dbSNP:752758599
247 247 c, t dbSNP:781631751
248 248 a, c dbSNP:755241254
252 252 c, t dbSNP:370490787
263 263 -, gccatc dbSNP:764462723
282 282 a, g dbSNP:747253986
294 294 a, g, t dbSNP:758790721
295 295 a, g dbSNP:750744461
296 296 a, g dbSNP:765830204
297 297 -, gaa dbSNP:771230803
311 311 a, g dbSNP:376573289
318 318 a, c, g dbSNP:763623534
319 319 a, g dbSNP:17855203
323 323 a, t dbSNP:760130608
327 327 c, t dbSNP:150267054
336 336 a, c dbSNP:368094683
339 339 c, t dbSNP:759442724
346 346 a, g dbSNP:774007780
348 348 c, t dbSNP:746355265
364 364 a, g dbSNP:749210341
372 372 a, c, t dbSNP:142659098
373 373 c, t dbSNP:147718886
389 389 a, g dbSNP:780386537
406 406 c, t dbSNP:372588506
409 409 a, t dbSNP:746293406
419 419 a, g dbSNP:765051974
428 428 c, t dbSNP:757576097
433 433 a, g dbSNP:571028390
436 436 c, g dbSNP:778196890
448 448 c, t dbSNP:370202240
460 460 c, t dbSNP:145598174
462 462 a, g dbSNP:771300057
468 468 c, g dbSNP:189052337
475 475 a, g dbSNP:778319588
487 487 c, t dbSNP:368633795
496 496 c, t dbSNP:752305781
500 500 a, g dbSNP:780541101
522 522 a, t dbSNP:754474625
523 523 a, g dbSNP:5745635
525 525 g, t dbSNP:766059183
537 537 a, t dbSNP:151170133
544 544 c, t dbSNP:769553578
546 546 a, t dbSNP:750225776
562 562 c, t dbSNP:537365064
563 563 a, g dbSNP:781690781
577 577 a, c dbSNP:754448554
579 579 c, t dbSNP:141389863
582 582 a, g dbSNP:779906703
585 585 a, g dbSNP:758097772
591 591 a, g dbSNP:750282888
601 601 a, g dbSNP:765155428
610 610 a, g dbSNP:757026543
634 634 -, a dbSNP:771780436
636 636 a, g dbSNP:753731630
640 640 a, g dbSNP:764026915
648 648 g, t dbSNP:17566
661 661 a, g dbSNP:142045938
664 664 c, t dbSNP:367553438
675 675 a, g dbSNP:762140462
677 677 c, t dbSNP:777026877
678 678 a, g dbSNP:773949476
682 682 c, t dbSNP:540789414
699 699 a, g dbSNP:73381183
701 701 c, t dbSNP:747549889
708 708 a, g dbSNP:776090604
716 716 a, c dbSNP:771720187
731 731 a, g dbSNP:745308839
732 732 a, g dbSNP:778497868
753 753 a, g dbSNP:148615168
755 755 c, g dbSNP:749085781
764 764 c, t dbSNP:777593933
765 765 a, g dbSNP:558400297
769 769 c, t dbSNP:143891160
779 779 a, g dbSNP:781053176
795 795 c, t dbSNP:377663087
813 813 c, g dbSNP:144136982
834 834 a, g dbSNP:372600489
857 857 g, t dbSNP:139457161
874 874 a, g dbSNP:754102922
886 886 c, t dbSNP:5745666
890 890 a, g dbSNP:761186765
892 892 a, g dbSNP:753099990
894 894 c, t dbSNP:768190212
896 896 c, t dbSNP:760088738
899 899 c, t dbSNP:202185530
900 900 a, g dbSNP:200559590
904 904 c, t dbSNP:762316469
913 913 a, g dbSNP:573737350
916 916 c, t dbSNP:772926410
928 928 c, t dbSNP:755250749
929 929 a, g dbSNP:752123971
933 933 a, g dbSNP:559265540
936 936 c, g, t dbSNP:560215969
937 937 c, t dbSNP:772684432
948 948 a, c dbSNP:764905780
950 950 c, t dbSNP:761481749
952 952 c, t dbSNP:776214617
955 955 c, t dbSNP:375177081
957 957 a, g dbSNP:768451705
964 964 c, t dbSNP:147312555
969 969 c, g dbSNP:775564237
975 975 c, t dbSNP:771889279
977 977 a, g dbSNP:749434892
1006 1006 a, c dbSNP:777970277
1007 1007 a, t dbSNP:754578668
1010 1010 c, t dbSNP:756224070
1011 1011 g, t dbSNP:371502313
1024 1024 g, t dbSNP:533086523
1025 1025 a, g dbSNP:755376784
1029 1029 c, t dbSNP:751870029
1035 1035 c, t dbSNP:766971992
1036 1036 a, g dbSNP:368002816
1039 1039 c, t dbSNP:374136304
1040 1040 a, g dbSNP:764746019
1045 1045 c, t dbSNP:778286006
1047 1047 a, c dbSNP:755584740
1052 1052 a, g dbSNP:747693036
1053 1053 a, t dbSNP:780768372
1055 1055 a, t dbSNP:754659494
1058 1058 c, g dbSNP:367811213
1060 1060 a, c, t dbSNP:141751290
1063 1063 a, t dbSNP:750329013
1068 1068 g, t dbSNP:775912518
1071 1071 c, t dbSNP:760721822
1080 1080 c, t dbSNP:770152785
1085 1085 a, g dbSNP:5745687
1092 1092 a, t dbSNP:767766226
1095 1095 a, c dbSNP:759784966
1096 1096 a, g dbSNP:774508303
1107 1107 a, g dbSNP:771299734
1109 1109 a, g dbSNP:749600508
1111 1111 c, t dbSNP:773660386
1115 1115 a, t dbSNP:781437504
1123 1123 g, t dbSNP:770323794
1132 1132 c, t dbSNP:747664002
1135 1135 c, t dbSNP:780821031
1157 1157 c, t dbSNP:754502188
1158 1158 a, g, t dbSNP:374484762
1159 1159 c, t dbSNP:779783865
1163 1163 g, t dbSNP:5745688
1164 1164 a, t dbSNP:750217931
1174 1174 c, t dbSNP:569559437
1180 1180 c, t dbSNP:757186641
1181 1181 a, g dbSNP:549654942
1183 1183 a, g dbSNP:148714837
1204 1204 c, t dbSNP:759555986
1210 1210 a, g dbSNP:751786738
1212 1212 a, g dbSNP:766585116
1218 1218 a, g dbSNP:763206701
1223 1223 a, c dbSNP:373442319
1224 1224 a, g dbSNP:750575553
1225 1225 a, g dbSNP:547103298
1248 1248 a, g dbSNP:184858341
1255 1255 c, t dbSNP:765660542
1258 1258 a, t dbSNP:762012189
1266 1266 a, g dbSNP:5745703
1273 1273 c, t dbSNP:777341007
1289 1289 a, t dbSNP:769270957
1303 1303 c, t dbSNP:112557787
1310 1310 a, c dbSNP:200948659
1313 1313 a, g dbSNP:771441595
1325 1325 a, g dbSNP:745555932
1326 1326 a, g dbSNP:778372935
1328 1328 a, g dbSNP:770765351
1334 1334 a, c dbSNP:749078919
1335 1335 a, t dbSNP:777748021
1352 1352 c, t dbSNP:758567520
1353 1353 a, g dbSNP:745919057
1354 1354 c, t dbSNP:377036882
1362 1362 c, g dbSNP:745854997
1369 1369 c, t dbSNP:754071169
1376 1376 a, g dbSNP:764574926
1378 1378 c, t dbSNP:756489380
1405 1405 a, g dbSNP:373298029
1437 1437 a, c dbSNP:767907135
1445 1445 c, t dbSNP:759145490
1450 1450 c, t dbSNP:755416276
1466 1466 c, g dbSNP:61735997
1469 1469 a, g dbSNP:752052736
1499 1499 a, c dbSNP:765902946
1517 1517 c, g dbSNP:762379250
1534 1534 a, c dbSNP:750085970
1536 1536 c, t dbSNP:764869064
1537 1537 a, g dbSNP:761711142
1541 1541 a, c dbSNP:776270506
1549 1549 c, t dbSNP:566128293
1555 1555 c, t dbSNP:760563094
1563 1563 a, g dbSNP:775433097
1571 1571 a, t dbSNP:141487191
1578 1578 a, g dbSNP:148293411
1597 1597 a, g dbSNP:764922029
1619 1619 c, t dbSNP:761396857
1624 1624 c, t dbSNP:375872396
1625 1625 a, g, t dbSNP:571102402
1627 1627 a, g dbSNP:759566751
1629 1629 c, t dbSNP:397516446
1631 1631 c, t dbSNP:774389172
1641 1641 a, g dbSNP:371520373
1645 1645 a, g, t dbSNP:372075114
1652 1652 c, t dbSNP:768896096
1679 1679 -, c dbSNP:748881407
1682 1682 a, g dbSNP:747426081
1683 1683 c, g dbSNP:775857995
1688 1688 a, g dbSNP:772388735
1696 1696 a, g dbSNP:200549396
1699 1699 g, t dbSNP:746380080
1703 1703 a, g dbSNP:779336001
1707 1707 c, t dbSNP:756776269
1712 1712 c, t dbSNP:572203404
1728 1728 c, t dbSNP:776754331
1738 1738 a, t dbSNP:764035851
1753 1753 c, g dbSNP:143262934
1756 1756 c, t dbSNP:775625219
1765 1765 a, t dbSNP:772441910
1769 1769 a, g dbSNP:759881207
1772 1772 c, t dbSNP:527951814
1773 1773 a, g, t dbSNP:771437724
1774 1774 a, g dbSNP:748796647
1789 1789 a, t dbSNP:368519009
1802 1802 g, t dbSNP:769438908
1824 1824 a, t dbSNP:192078086
1827 1827 a, c, g dbSNP:752861058
1837 1837 c, t dbSNP:148098479
1838 1838 a, g dbSNP:779933934
1847 1847 a, g dbSNP:370757602
1849 1849 c, g, t dbSNP:144086354
1850 1850 a, g dbSNP:778704152
1860 1860 a, g dbSNP:559990458
1862 1862 c, g dbSNP:376043671
1873 1873 c, t dbSNP:752743857
1879 1879 c, t dbSNP:138548003
1883 1883 c, t dbSNP:759776706
1888 1888 a, g dbSNP:751689885
1890 1890 a, g dbSNP:766769838
1900 1900 a, g dbSNP:763167149
1906 1906 a, g dbSNP:773824390
1920 1920 c, t dbSNP:765831517
1921 1921 a, g dbSNP:201395847
1939 1939 g, t dbSNP:531850775
1940 1940 a, g dbSNP:538415452
1948 1948 c, t dbSNP:199516810
1951 1951 c, t dbSNP:754944443
1962 1962 c, t dbSNP:751819844
1963 1963 a, g dbSNP:766493585
1968 1968 a, g dbSNP:758862083
1989 1989 c, t dbSNP:147075806
1991 1991 a, g dbSNP:765890500
1993 1993 c, t dbSNP:762445545
2004 2004 a, c dbSNP:777216192
2007 2007 c, g dbSNP:141774517
2013 2013 a, g dbSNP:760093179
2023 2023 c, g dbSNP:775281878
2028 2028 g, t dbSNP:771594786
2032 2032 c, t dbSNP:200374420
2034 2034 c, t dbSNP:774153258
2037 2037 c, g dbSNP:770672389
2041 2041 a, g dbSNP:376395424
2042 2042 a, g dbSNP:371826587
2044 2044 c, t dbSNP:755575139
2047 2047 a, c, t dbSNP:373545882
2055 2055 a, g dbSNP:770723689
2059 2059 a, g dbSNP:762721825
2060 2060 c, t dbSNP:773198515
2063 2063 c, t dbSNP:769585774
2064 2064 a, g dbSNP:747158538
2066 2066 a, g dbSNP:145494248
2073 2073 a, c, g dbSNP:746110664
2075 2075 a, c dbSNP:779267373
2079 2079 a, g dbSNP:565056780
2081 2081 a, g dbSNP:754226152
2092 2092 c, t dbSNP:778395987
2094 2094 a, g dbSNP:756602776
2109 2109 a, g dbSNP:202215700
2114 2114 c, t dbSNP:545249104
2117 2117 a, g dbSNP:150109563
2119 2119 a, g dbSNP:369418750
2129 2129 c, t dbSNP:751270979
2140 2140 c, t dbSNP:766007676
2154 2154 c, g, t dbSNP:772967560
2156 2156 a, g dbSNP:769580628
2165 2165 a, t dbSNP:761664295
2169 2169 g, t dbSNP:776576073
2182 2182 c, t dbSNP:772105726
2187 2187 a, g dbSNP:770114698
2194 2194 c, t dbSNP:367800414
2200 2200 c, t dbSNP:781586687
2203 2203 a, g dbSNP:755450918
2207 2207 c, g dbSNP:747463316
2213 2213 a, g dbSNP:544960907
2216 2216 c, g dbSNP:779568330
2234 2234 g, t dbSNP:757849670
2245 2245 a, c dbSNP:750104031
2257 2257 a, t dbSNP:563705580
2259 2259 a, g dbSNP:543827901
2270 2270 a, g dbSNP:372366408
2280 2280 a, g dbSNP:763933267
2285 2285 a, g dbSNP:760728215
2289 2289 g, t dbSNP:775503666
2297 2297 c, t dbSNP:201768315
2298 2298 a, g dbSNP:763072412
2305 2305 a, c, g dbSNP:267601578
2311 2311 c, t dbSNP:770025635
2314 2314 a, t dbSNP:764730119
2341 2341 a, g dbSNP:561484014
2344 2344 c, t dbSNP:777071855
2354 2354 c, t dbSNP:140790665
2358 2358 a, c dbSNP:747516423
2360 2360 c, t dbSNP:780528260
2362 2362 a, g dbSNP:757972650
2368 2368 a, g dbSNP:745356078
2370 2370 -, aagt dbSNP:779001317
2372 2372 a, g dbSNP:778483338
2384 2384 c, t dbSNP:756957205
2391 2391 a, c dbSNP:753392862
2393 2393 a, g dbSNP:777717109
2398 2398 a, g dbSNP:767332482
2402 2402 a, c, t dbSNP:377185441
2407 2407 c, t dbSNP:767556298
2410 2410 a, c, g dbSNP:750490813
2411 2411 c, t dbSNP:765177187
2419 2419 g, t dbSNP:542402530
2428 2428 a, g dbSNP:573406409
2464 2464 -, aact dbSNP:374789745
2530 2530 c, t dbSNP:531222230
2583 2583 a, g dbSNP:544879896
2586 2586 a, g dbSNP:539702697
2621 2621 a, c dbSNP:777313117
2628 2628 -, ca dbSNP:763357973
2628 2628 c, g dbSNP:577625800
2634 2634 a, t dbSNP:758013381
2664 2664 a, t dbSNP:186434043
2676 2676 -, taat dbSNP:545984462
2715 2715 a, g dbSNP:537468151
2749 2749 c, t dbSNP:568550366
2758 2758 a, t dbSNP:182057547
2776 2776 a, c dbSNP:535633453
2814 2814 c, g dbSNP:778665027
2825 2825 a, t dbSNP:566500466
2829 2829 a, g dbSNP:5745768
2854 2854 a, t dbSNP:533030741
2884 2884 c, g dbSNP:754673292
2885 2885 -, tt dbSNP:5745769
2888 2888 -, tt dbSNP:567921457
2916 2916 a, g dbSNP:759206756
2931 2931 a, t dbSNP:753671589
2950 2950 a, g dbSNP:766188562
2959 2959 c, t dbSNP:140696262
2960 2960 a, g dbSNP:756101495
2974 2974 c, g dbSNP:75716542
3018 3018 a, c dbSNP:5745770
3019 3019 a, c dbSNP:561820689
3056 3056 g, t dbSNP:750511884
3104 3104 a, c dbSNP:541515764
3112 3112 -, ctt dbSNP:777098170
3137 3137 c, t dbSNP:537478018
3170 3170 g, t dbSNP:574028648
3176 3176 c, g dbSNP:559655621
3181 3181 a, g dbSNP:776314803
3196 3196 a, t dbSNP:546438057
3295 3295 c, t dbSNP:577589184
3320 3320 g, t dbSNP:189832870
3336 3336 a, c dbSNP:185299752
3342 3342 a, g dbSNP:192611863
3371 3371 a, g dbSNP:5745772
3396 3396 a, g dbSNP:5745773
3451 3451 c, t dbSNP:184092464
3458 3458 a, t dbSNP:28677052
3464 3464 a, g dbSNP:775942537
3477 3477 a, g dbSNP:760241049
3522 3522 a, g dbSNP:566188748
3525 3525 a, c dbSNP:546456660
3529 3529 a, g dbSNP:578009350
3627 3627 a, c dbSNP:539282857
3632 3632 a, g dbSNP:5745774
3663 3663 -, t dbSNP:538206512
3664 3664 g, t dbSNP:772057333
3679 3679 c, t dbSNP:191265665
3745 3745 a, c dbSNP:542200179
3775 3775 c, t dbSNP:746412693
3784 3784 c, g dbSNP:530719432
3786 3786 a, g dbSNP:567925018
3879 3879 c, g dbSNP:4732402
3883 3883 a, g dbSNP:771628734
3890 3890 a, g dbSNP:5745775
3911 3911 a, g dbSNP:186791770
3922 3922 c, t dbSNP:559424345
3930 3930 a, c dbSNP:554838290
3953 3953 a, c dbSNP:555372511
3984 3984 c, t dbSNP:5745776
4017 4017 c, t dbSNP:5745777
4038 4038 c, g dbSNP:535744424
4040 4040 g, t dbSNP:5745778
4050 4050 a, g dbSNP:544081230
4078 4078 a, g dbSNP:768386941
4105 4105 c, t dbSNP:566864574
4141 4141 a, t dbSNP:575086470
4153 4153 g, t dbSNP:554639010
4166 4166 g, t dbSNP:10262924
4171 4171 -, tt dbSNP:373238903
4211 4211 c, t dbSNP:748988745
4247 4247 a, c dbSNP:183089338
4269 4269 a, g dbSNP:558667567
4272 4272 -, ctc dbSNP:67776924
4274 4274 -, cct dbSNP:5745779
4289 4289 a, g dbSNP:539248931
4326 4326 c, t dbSNP:570267972
4338 4338 c, t dbSNP:5745780
4394 4394 a, g dbSNP:5745781
4447 4447 c, g dbSNP:756092978
4531 4531 a, g dbSNP:73155686
4580 4580 c, t dbSNP:568363721
4581 4581 a, g dbSNP:144029351
4592 4592 c, t dbSNP:5745782
4616 4616 a, g dbSNP:193254527
4647 4647 g, t dbSNP:552347951
4687 4687 c, g dbSNP:750454785
4750 4750 g, t dbSNP:532246490
4809 4809 a, c dbSNP:547131850
4834 4834 c, t dbSNP:757357420
4836 4836 g, t dbSNP:563830772
4842 4842 a, c dbSNP:188253748
4871 4871 a, g dbSNP:530308510
4884 4884 a, g dbSNP:146217138
4935 4935 a, c dbSNP:764169368
4966 4966 a, g dbSNP:763282702
5084 5084 c, t dbSNP:571285366
5093 5093 a, g dbSNP:752971849
5106 5106 c, g dbSNP:142379914
5146 5146 -, a dbSNP:34075472
5152 5152 g, t dbSNP:572453182
5168 5168 a, t dbSNP:148783522
5193 5193 a, t dbSNP:545061143
5196 5196 a, c, t dbSNP:149351591
5239 5239 a, t dbSNP:569697536
5256 5256 a, g dbSNP:538570220
5285 5285 c, t dbSNP:569458327
5297 5297 g, t dbSNP:537276806
5327 5327 c, t dbSNP:531462048
5328 5328 a, t dbSNP:777064011
5357 5357 a, g dbSNP:781609608
5431 5431 -, a dbSNP:757549796
5466 5466 g, t dbSNP:183726882
5649 5649 a, t dbSNP:367964046
5764 5764 a, t dbSNP:5745783
5778 5778 a, t dbSNP:1743
5879 5879 -, gtt dbSNP:777658790
5903 5903 a, t dbSNP:565624689
5927 5927 a, t dbSNP:79911513
5930 5930 a, g dbSNP:5745784
5935 5935 a, g dbSNP:5745785

Target ORF information:

RefSeq Version XM_011516115
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011516115

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTATCGGG GTAAAGACCT ACAGGAAAAC TACTGTCGAA ATCCTCGAGG GGAAGAAGGG
GGACCCTGGT GTTTCACAAG CAATCCAGAG GTACGCTACG AAGTCTGTGA CATTCCTCAG
TGTTCAGAAG TTGAATGCAT GACCTGCAAT GGGGAGAGTT ATCGAGGTCT CATGGATCAT
ACAGAATCAG GCAAGATTTG TCAGCGCTGG GATCATCAGA CACCACACCG GCACAAATTC
TTGCCTGAAA GATATCCCGA CAAGGGCTTT GATGATAATT ATTGCCGCAA TCCCGATGGC
CAGCCGAGGC CATGGTGCTA TACTCTTGAC CCTCACACCC GCTGGGAGTA CTGTGCAATT
AAAACATGCG CTGACAATAC TATGAATGAC ACTGATGTTC CTTTGGAAAC AACTGAATGC
ATCCAAGGTC AAGGAGAAGG CTACAGGGGC ACTGTCAATA CCATTTGGAA TGGAATTCCA
TGTCAGCGTT GGGATTCTCA GTATCCTCAC GAGCATGACA TGACTCCTGA AAATTTCAAG
TGCAAGGACC TACGAGAAAA TTACTGCCGA AATCCAGATG GGTCTGAATC ACCCTGGTGT
TTTACCACTG ATCCAAACAT CCGAGTTGGC TACTGCTCCC AAATTCCAAA CTGTGATATG
TCACATGGAC AAGATTGTTA TCGTGGGAAT GGCAAAAATT ATATGGGCAA CTTATCCCAA
ACAAGATCTG GACTAACATG TTCAATGTGG GACAAGAACA TGGAAGACTT ACATCGTCAT
ATCTTCTGGG AACCAGATGC AAGTAAGCTG AATGAGAATT ACTGCCGAAA TCCAGATGAT
GATGCTCATG GACCCTGGTG CTACACGGGA AATCCACTCA TTCCTTGGGA TTATTGCCCT
ATTTCTCGTT GTGAAGGTGA TACCACACCT ACAATAGTCA ATTTAGACCA TCCCGTAATA
TCTTGTGCCA AAACGAAACA ATTGCGAGTT GTAAATGGGA TTCCAACACG AACAAACATA
GGATGGATGG TTAGTTTGAG ATACAGAAAT AAACATATCT GCGGAGGATC ATTGATAAAG
GAGAGTTGGG TTCTTACTGC ACGACAGTGT TTCCCTTCTC GAGACTTGAA AGATTATGAA
GCTTGGCTTG GAATTCATGA TGTCCACGGA AGAGGAGATG AGAAATGCAA ACAGGTTCTC
AATGTTTCCC AGCTGGTATA TGGCCCTGAA GGATCAGATC TGGTTTTAAT GAAGCTTGCC
AGGCCTGCTG TCCTGGATGA TTTTGTTAGT ACGATTGATT TACCTAATTA TGGATGCACA
ATTCCTGAAA AGACCAGTTG CAGTGTTTAT GGCTGGGGCT ACACTGGATT GATCAACTAT
GATGGCCTAT TACGAGTGGC ACATCTCTAT ATAATGGGAA ATGAGAAATG CAGCCAGCAT
CATCGAGGGA AGGTGACTCT GAATGAGTCT GAAATATGTG CTGGGGCTGA AAAGATTGGA
TCAGGACCAT GTGAGGGGGA TTATGGTGGC CCACTTGTTT GTGAGCAACA TAAAATGAGA
ATGGTTCTTG GTGTCATTGT TCCTGGTCGT GGATGTGCCA TTCCAAATCG TCCTGGTATT
TTTGTCCGAG TAGCATATTA TGCAAAATGG ATACACAAAA TTATTTTAAC ATATAAGGTA
CCACAGTCATT AG

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu25798D
Sequence Information ORF Nucleotide Sequence (Length: 873bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product hepatocyte growth factor isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU533235.1, L02931.1 and BM971901.1. Summary: Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (2) lacks multiple 3' exons but includes an alternate 3' exon, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus, compared to isoform 1. Isoform 2, also named NK2, has been shown to act as a competitive antagonist to active hepatocyte growth factor for the c-Met receptor. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X57574.1, M77227.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA GAGTTATCGA
GGTCTCATGG ATCATACAGA ATCAGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCA
CACCGGCACA AATTCTTGCC TGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC
CGCAATCCCG ATGGCCAGCC GAGGCCATGG TGCTATACTC TTGACCCTCA CACCCGCTGG
GAGTACTGTG CAATTAAAAC ATGCGAGACA TAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001010931.1
CDS166..1038
Misc Feature(1)58..60(+)
Misc Feature(2)280..531(+)
Misc Feature(3)343..384(+)
Misc Feature(4)541..786(+)
Misc Feature(5)574..576(+)
Misc Feature(6)619..759(+)
Misc Feature(7)787..1032(+)
Misc Feature(8)823..825(+)
Misc Feature(9)868..1005(+)
Exon (1)1..253
Gene:HGF
Gene Synonym:
Exon (2)254..419
Gene:HGF
Gene Synonym:
Exon (3)420..532
Gene:HGF
Gene Synonym:
Exon (4)533..647
Gene:HGF
Gene Synonym:
Exon (5)648..790
Gene:HGF
Gene Synonym:
Exon (6)791..911
Gene:HGF
Gene Synonym:
Exon (7)912..1030
Gene:HGF
Gene Synonym:
Exon (8)1031..1289
Gene:HGF
Gene Synonym:
Translation
Position Chain Variation Link
24 24 c, g dbSNP:371718041
25 25 a, g dbSNP:545903754
32 32 c, t dbSNP:577719518
66 66 a, c dbSNP:76382276
72 72 a, c dbSNP:5745609
77 77 c, g dbSNP:575483475
81 81 a, g dbSNP:555377705
122 122 a, g dbSNP:758149409
125 125 a, c dbSNP:768292342
126 126 a, t dbSNP:28719812
149 149 g, t dbSNP:779801717
151 151 c, t dbSNP:370842395
152 152 c, g dbSNP:201598927
169 169 c, t dbSNP:375901177
171 171 g, t dbSNP:745851853
173 173 a, t dbSNP:778667611
192 192 c, t dbSNP:372751973
213 213 c, t dbSNP:148935472
217 217 c, t dbSNP:752758599
222 222 c, t dbSNP:781631751
223 223 a, c dbSNP:755241254
227 227 c, t dbSNP:370490787
238 238 -, gccatc dbSNP:764462723
257 257 a, g dbSNP:747253986
269 269 a, g, t dbSNP:758790721
270 270 a, g dbSNP:750744461
271 271 a, g dbSNP:765830204
272 272 -, gaa dbSNP:771230803
286 286 a, g dbSNP:376573289
293 293 a, c, g dbSNP:763623534
294 294 a, g dbSNP:17855203
298 298 a, t dbSNP:760130608
302 302 c, t dbSNP:150267054
311 311 a, c dbSNP:368094683
314 314 c, t dbSNP:759442724
321 321 a, g dbSNP:774007780
323 323 c, t dbSNP:746355265
339 339 a, g dbSNP:749210341
347 347 a, c, t dbSNP:142659098
348 348 c, t dbSNP:147718886
364 364 a, g dbSNP:780386537
381 381 c, t dbSNP:372588506
384 384 a, t dbSNP:746293406
394 394 a, g dbSNP:765051974
403 403 c, t dbSNP:757576097
408 408 a, g dbSNP:571028390
411 411 c, g dbSNP:778196890
423 423 c, t dbSNP:370202240
435 435 c, t dbSNP:145598174
437 437 a, g dbSNP:771300057
443 443 c, g dbSNP:189052337
450 450 a, g dbSNP:778319588
462 462 c, t dbSNP:368633795
471 471 c, t dbSNP:752305781
475 475 a, g dbSNP:780541101
497 497 a, t dbSNP:754474625
498 498 a, g dbSNP:5745635
500 500 g, t dbSNP:766059183
512 512 a, t dbSNP:151170133
519 519 c, t dbSNP:769553578
521 521 a, t dbSNP:750225776
537 537 c, t dbSNP:537365064
538 538 a, g dbSNP:781690781
552 552 a, c dbSNP:754448554
554 554 c, t dbSNP:141389863
557 557 a, g dbSNP:779906703
560 560 a, g dbSNP:758097772
566 566 a, g dbSNP:750282888
576 576 a, g dbSNP:765155428
585 585 a, g dbSNP:757026543
609 609 -, a dbSNP:771780436
611 611 a, g dbSNP:753731630
615 615 a, g dbSNP:764026915
623 623 g, t dbSNP:17566
636 636 a, g dbSNP:142045938
639 639 c, t dbSNP:367553438
648 648 c, t dbSNP:762973783
659 659 c, t dbSNP:370760302
660 660 a, g dbSNP:137853235
662 662 a, g dbSNP:373660832
665 665 a, g dbSNP:762140462
667 667 c, t dbSNP:777026877
668 668 a, g dbSNP:773949476
672 672 c, t dbSNP:540789414
689 689 a, g dbSNP:73381183
691 691 c, t dbSNP:747549889
698 698 a, g dbSNP:776090604
706 706 a, c dbSNP:771720187
721 721 a, g dbSNP:745308839
722 722 a, g dbSNP:778497868
743 743 a, g dbSNP:148615168
745 745 c, g dbSNP:749085781
754 754 c, t dbSNP:777593933
755 755 a, g dbSNP:558400297
759 759 c, t dbSNP:143891160
769 769 a, g dbSNP:781053176
785 785 c, t dbSNP:377663087
803 803 c, g dbSNP:144136982
824 824 a, g dbSNP:372600489
847 847 g, t dbSNP:139457161
864 864 a, g dbSNP:754102922
876 876 c, t dbSNP:5745666
880 880 a, g dbSNP:761186765
882 882 a, g dbSNP:753099990
884 884 c, t dbSNP:768190212
886 886 c, t dbSNP:760088738
889 889 c, t dbSNP:202185530
890 890 a, g dbSNP:200559590
894 894 c, t dbSNP:762316469
903 903 a, g dbSNP:573737350
906 906 c, t dbSNP:772926410
918 918 c, t dbSNP:755250749
919 919 a, g dbSNP:752123971
923 923 a, g dbSNP:559265540
926 926 c, g, t dbSNP:560215969
927 927 c, t dbSNP:772684432
938 938 a, c dbSNP:764905780
940 940 c, t dbSNP:761481749
942 942 c, t dbSNP:776214617
945 945 c, t dbSNP:375177081
947 947 a, g dbSNP:768451705
954 954 c, t dbSNP:147312555
959 959 c, g dbSNP:775564237
965 965 c, t dbSNP:771889279
967 967 a, g dbSNP:749434892
996 996 a, c dbSNP:777970277
997 997 a, t dbSNP:754578668
1000 1000 c, t dbSNP:756224070
1001 1001 g, t dbSNP:371502313
1014 1014 g, t dbSNP:533086523
1015 1015 a, g dbSNP:755376784
1019 1019 c, t dbSNP:751870029
1025 1025 c, t dbSNP:766971992
1026 1026 a, g dbSNP:368002816
1029 1029 c, t dbSNP:374136304
1030 1030 a, g dbSNP:764746019
1031 1031 a, t dbSNP:756825686
1035 1035 a, g dbSNP:753332987
1038 1038 -, a dbSNP:534250947
1040 1040 a, g dbSNP:374272330
1057 1057 a, g dbSNP:760213927
1063 1063 c, t dbSNP:372039959
1068 1068 -, tct dbSNP:752761642
1081 1081 a, c dbSNP:768604458
1091 1091 a, g dbSNP:759348079
1095 1095 a, c dbSNP:774438294
1096 1096 -, gaa dbSNP:765232388
1138 1138 c, t dbSNP:749331243
1140 1140 c, t dbSNP:5745676
1170 1170 g, t dbSNP:145710222
1182 1182 a, c dbSNP:117646126
1211 1211 a, g dbSNP:781670329
1217 1217 c, t dbSNP:5745677
1234 1234 a, g dbSNP:747471739
1249 1249 c, t dbSNP:5745678
1254 1254 c, t dbSNP:189833202
1255 1255 c, t dbSNP:564182731

Target ORF information:

RefSeq Version NM_001010931
Organism Homo sapiens (human)
Definition Homo sapiens hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001010931

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA GAGTTATCGA
GGTCTCATGG ATCATACAGA ATCAGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCA
CACCGGCACA AATTCTTGCC TGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC
CGCAATCCCG ATGGCCAGCC GAGGCCATGG TGCTATACTC TTGACCCTCA CACCCGCTGG
GAGTACTGTG CAATTAAAAC ATGCGAGACA TAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu26016D
Sequence Information ORF Nucleotide Sequence (Length: 858bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product hepatocyte growth factor isoform 4 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU533235.1, BC022308.1, L02931.1 and BM971901.1. Summary: Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (4) lacks an internal in-frame segment and multiple 3' exons but includes an alternate 3' exon, compared to variant 1. The resulting protein (isoform 4) lacks an internal segment and has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC022308.1, AL549164.3 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTATCGGG GTAAAGACCT ACAGGAAAAC TACTGTCGAA ATCCTCGAGG GGAAGAAGGG
GGACCCTGGT GTTTCACAAG CAATCCAGAG GTACGCTACG AAGTCTGTGA CATTCCTCAG
TGTTCAGAAG TTGAATGCAT GACCTGCAAT GGGGAGAGTT ATCGAGGTCT CATGGATCAT
ACAGAATCAG GCAAGATTTG TCAGCGCTGG GATCATCAGA CACCACACCG GCACAAATTC
TTGCCTGAAA GATATCCCGA CAAGGGCTTT GATGATAATT ATTGCCGCAA TCCCGATGGC
CAGCCGAGGC CATGGTGCTA TACTCTTGAC CCTCACACCC GCTGGGAGTA CTGTGCAATT
AAAACATGCG AGACATAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001010933.1
CDS166..1023
Misc Feature(1)58..60(+)
Misc Feature(2)280..531(+)
Misc Feature(3)343..384(+)
Misc Feature(4)541..771(+)
Misc Feature(5)574..576(+)
Misc Feature(6)619..744(+)
Misc Feature(7)772..1017(+)
Misc Feature(8)808..810(+)
Misc Feature(9)853..990(+)
Exon (1)1..253
Gene:HGF
Gene Synonym:
Exon (2)254..419
Gene:HGF
Gene Synonym:
Exon (3)420..532
Gene:HGF
Gene Synonym:
Exon (4)533..647
Gene:HGF
Gene Synonym:
Exon (5)648..775
Gene:HGF
Gene Synonym:
Exon (6)776..896
Gene:HGF
Gene Synonym:
Exon (7)897..1015
Gene:HGF
Gene Synonym:
Exon (8)1016..1274
Gene:HGF
Gene Synonym:
Translation
Position Chain Variation Link
24 24 c, g dbSNP:371718041
25 25 a, g dbSNP:545903754
32 32 c, t dbSNP:577719518
66 66 a, c dbSNP:76382276
72 72 a, c dbSNP:5745609
77 77 c, g dbSNP:575483475
81 81 a, g dbSNP:555377705
122 122 a, g dbSNP:758149409
125 125 a, c dbSNP:768292342
126 126 a, t dbSNP:28719812
149 149 g, t dbSNP:779801717
151 151 c, t dbSNP:370842395
152 152 c, g dbSNP:201598927
169 169 c, t dbSNP:375901177
171 171 g, t dbSNP:745851853
173 173 a, t dbSNP:778667611
192 192 c, t dbSNP:372751973
213 213 c, t dbSNP:148935472
217 217 c, t dbSNP:752758599
222 222 c, t dbSNP:781631751
223 223 a, c dbSNP:755241254
227 227 c, t dbSNP:370490787
238 238 -, gccatc dbSNP:764462723
257 257 a, g dbSNP:747253986
269 269 a, g, t dbSNP:758790721
270 270 a, g dbSNP:750744461
271 271 a, g dbSNP:765830204
272 272 -, gaa dbSNP:771230803
286 286 a, g dbSNP:376573289
293 293 a, c, g dbSNP:763623534
294 294 a, g dbSNP:17855203
298 298 a, t dbSNP:760130608
302 302 c, t dbSNP:150267054
311 311 a, c dbSNP:368094683
314 314 c, t dbSNP:759442724
321 321 a, g dbSNP:774007780
323 323 c, t dbSNP:746355265
339 339 a, g dbSNP:749210341
347 347 a, c, t dbSNP:142659098
348 348 c, t dbSNP:147718886
364 364 a, g dbSNP:780386537
381 381 c, t dbSNP:372588506
384 384 a, t dbSNP:746293406
394 394 a, g dbSNP:765051974
403 403 c, t dbSNP:757576097
408 408 a, g dbSNP:571028390
411 411 c, g dbSNP:778196890
423 423 c, t dbSNP:370202240
435 435 c, t dbSNP:145598174
437 437 a, g dbSNP:771300057
443 443 c, g dbSNP:189052337
450 450 a, g dbSNP:778319588
462 462 c, t dbSNP:368633795
471 471 c, t dbSNP:752305781
475 475 a, g dbSNP:780541101
497 497 a, t dbSNP:754474625
498 498 a, g dbSNP:5745635
500 500 g, t dbSNP:766059183
512 512 a, t dbSNP:151170133
519 519 c, t dbSNP:769553578
521 521 a, t dbSNP:750225776
537 537 c, t dbSNP:537365064
538 538 a, g dbSNP:781690781
552 552 a, c dbSNP:754448554
554 554 c, t dbSNP:141389863
557 557 a, g dbSNP:779906703
560 560 a, g dbSNP:758097772
566 566 a, g dbSNP:750282888
576 576 a, g dbSNP:765155428
585 585 a, g dbSNP:757026543
609 609 -, a dbSNP:771780436
611 611 a, g dbSNP:753731630
615 615 a, g dbSNP:764026915
623 623 g, t dbSNP:17566
636 636 a, g dbSNP:142045938
639 639 c, t dbSNP:367553438
650 650 a, g dbSNP:762140462
652 652 c, t dbSNP:777026877
653 653 a, g dbSNP:773949476
657 657 c, t dbSNP:540789414
674 674 a, g dbSNP:73381183
676 676 c, t dbSNP:747549889
683 683 a, g dbSNP:776090604
691 691 a, c dbSNP:771720187
706 706 a, g dbSNP:745308839
707 707 a, g dbSNP:778497868
728 728 a, g dbSNP:148615168
730 730 c, g dbSNP:749085781
739 739 c, t dbSNP:777593933
740 740 a, g dbSNP:558400297
744 744 c, t dbSNP:143891160
754 754 a, g dbSNP:781053176
770 770 c, t dbSNP:377663087
788 788 c, g dbSNP:144136982
809 809 a, g dbSNP:372600489
832 832 g, t dbSNP:139457161
849 849 a, g dbSNP:754102922
861 861 c, t dbSNP:5745666
865 865 a, g dbSNP:761186765
867 867 a, g dbSNP:753099990
869 869 c, t dbSNP:768190212
871 871 c, t dbSNP:760088738
874 874 c, t dbSNP:202185530
875 875 a, g dbSNP:200559590
879 879 c, t dbSNP:762316469
888 888 a, g dbSNP:573737350
891 891 c, t dbSNP:772926410
903 903 c, t dbSNP:755250749
904 904 a, g dbSNP:752123971
908 908 a, g dbSNP:559265540
911 911 c, g, t dbSNP:560215969
912 912 c, t dbSNP:772684432
923 923 a, c dbSNP:764905780
925 925 c, t dbSNP:761481749
927 927 c, t dbSNP:776214617
930 930 c, t dbSNP:375177081
932 932 a, g dbSNP:768451705
939 939 c, t dbSNP:147312555
944 944 c, g dbSNP:775564237
950 950 c, t dbSNP:771889279
952 952 a, g dbSNP:749434892
981 981 a, c dbSNP:777970277
982 982 a, t dbSNP:754578668
985 985 c, t dbSNP:756224070
986 986 g, t dbSNP:371502313
999 999 g, t dbSNP:533086523
1000 1000 a, g dbSNP:755376784
1004 1004 c, t dbSNP:751870029
1010 1010 c, t dbSNP:766971992
1011 1011 a, g dbSNP:368002816
1014 1014 c, t dbSNP:374136304
1015 1015 a, g dbSNP:764746019
1016 1016 a, t dbSNP:756825686
1020 1020 a, g dbSNP:753332987
1023 1023 -, a dbSNP:534250947
1025 1025 a, g dbSNP:374272330
1042 1042 a, g dbSNP:760213927
1048 1048 c, t dbSNP:372039959
1053 1053 -, tct dbSNP:752761642
1066 1066 a, c dbSNP:768604458
1076 1076 a, g dbSNP:759348079
1080 1080 a, c dbSNP:774438294
1081 1081 -, gaa dbSNP:765232388
1123 1123 c, t dbSNP:749331243
1125 1125 c, t dbSNP:5745676
1155 1155 g, t dbSNP:145710222
1167 1167 a, c dbSNP:117646126
1196 1196 a, g dbSNP:781670329
1202 1202 c, t dbSNP:5745677
1219 1219 a, g dbSNP:747471739
1234 1234 c, t dbSNP:5745678
1239 1239 c, t dbSNP:189833202
1240 1240 c, t dbSNP:564182731

Target ORF information:

RefSeq Version NM_001010933
Organism Homo sapiens (human)
Definition Homo sapiens hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001010933

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTATCGGG GTAAAGACCT ACAGGAAAAC TACTGTCGAA ATCCTCGAGG GGAAGAAGGG
GGACCCTGGT GTTTCACAAG CAATCCAGAG GTACGCTACG AAGTCTGTGA CATTCCTCAG
TGTTCAGAAG TTGAATGCAT GACCTGCAAT GGGGAGAGTT ATCGAGGTCT CATGGATCAT
ACAGAATCAG GCAAGATTTG TCAGCGCTGG GATCATCAGA CACCACACCG GCACAAATTC
TTGCCTGAAA GATATCCCGA CAAGGGCTTT GATGATAATT ATTGCCGCAA TCCCGATGGC
CAGCCGAGGC CATGGTGCTA TACTCTTGAC CCTCACACCC GCTGGGAGTA CTGTGCAATT
AAAACATGCG AGACATAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu25927D
Sequence Information ORF Nucleotide Sequence (Length: 633bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product hepatocyte growth factor isoform 5 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU533235.1, U46010.1 and BC063485.1. Summary: Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (5) lacks multiple 3' exons but has an alternate 3' segment, compared to variant 1. The resulting protein (isoform 5) has a distinct and shorter C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC063485.1, AB208900.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGGTAAA TAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001010934.1
CDS166..798
Misc Feature(1)58..60(+)
Misc Feature(2)280..531(+)
Misc Feature(3)343..384(+)
Misc Feature(4)541..786(+)
Misc Feature(5)574..576(+)
Misc Feature(6)619..759(+)
Exon (1)1..253
Gene:HGF
Gene Synonym:
Exon (2)254..419
Gene:HGF
Gene Synonym:
Exon (3)420..532
Gene:HGF
Gene Synonym:
Exon (4)533..647
Gene:HGF
Gene Synonym:
Exon (5)648..2010
Gene:HGF
Gene Synonym:
Translation
Position Chain Variation Link
24 24 c, g dbSNP:371718041
25 25 a, g dbSNP:545903754
32 32 c, t dbSNP:577719518
66 66 a, c dbSNP:76382276
72 72 a, c dbSNP:5745609
77 77 c, g dbSNP:575483475
81 81 a, g dbSNP:555377705
122 122 a, g dbSNP:758149409
125 125 a, c dbSNP:768292342
126 126 a, t dbSNP:28719812
149 149 g, t dbSNP:779801717
151 151 c, t dbSNP:370842395
152 152 c, g dbSNP:201598927
169 169 c, t dbSNP:375901177
171 171 g, t dbSNP:745851853
173 173 a, t dbSNP:778667611
192 192 c, t dbSNP:372751973
213 213 c, t dbSNP:148935472
217 217 c, t dbSNP:752758599
222 222 c, t dbSNP:781631751
223 223 a, c dbSNP:755241254
227 227 c, t dbSNP:370490787
238 238 -, gccatc dbSNP:764462723
257 257 a, g dbSNP:747253986
269 269 a, g, t dbSNP:758790721
270 270 a, g dbSNP:750744461
271 271 a, g dbSNP:765830204
272 272 -, gaa dbSNP:771230803
286 286 a, g dbSNP:376573289
293 293 a, c, g dbSNP:763623534
294 294 a, g dbSNP:17855203
298 298 a, t dbSNP:760130608
302 302 c, t dbSNP:150267054
311 311 a, c dbSNP:368094683
314 314 c, t dbSNP:759442724
321 321 a, g dbSNP:774007780
323 323 c, t dbSNP:746355265
339 339 a, g dbSNP:749210341
347 347 a, c, t dbSNP:142659098
348 348 c, t dbSNP:147718886
364 364 a, g dbSNP:780386537
381 381 c, t dbSNP:372588506
384 384 a, t dbSNP:746293406
394 394 a, g dbSNP:765051974
403 403 c, t dbSNP:757576097
408 408 a, g dbSNP:571028390
411 411 c, g dbSNP:778196890
423 423 c, t dbSNP:370202240
435 435 c, t dbSNP:145598174
437 437 a, g dbSNP:771300057
443 443 c, g dbSNP:189052337
450 450 a, g dbSNP:778319588
462 462 c, t dbSNP:368633795
471 471 c, t dbSNP:752305781
475 475 a, g dbSNP:780541101
497 497 a, t dbSNP:754474625
498 498 a, g dbSNP:5745635
500 500 g, t dbSNP:766059183
512 512 a, t dbSNP:151170133
519 519 c, t dbSNP:769553578
521 521 a, t dbSNP:750225776
537 537 c, t dbSNP:537365064
538 538 a, g dbSNP:781690781
552 552 a, c dbSNP:754448554
554 554 c, t dbSNP:141389863
557 557 a, g dbSNP:779906703
560 560 a, g dbSNP:758097772
566 566 a, g dbSNP:750282888
576 576 a, g dbSNP:765155428
585 585 a, g dbSNP:757026543
609 609 -, a dbSNP:771780436
611 611 a, g dbSNP:753731630
615 615 a, g dbSNP:764026915
623 623 g, t dbSNP:17566
636 636 a, g dbSNP:142045938
639 639 c, t dbSNP:367553438
648 648 c, t dbSNP:762973783
659 659 c, t dbSNP:370760302
660 660 a, g dbSNP:137853235
662 662 a, g dbSNP:373660832
665 665 a, g dbSNP:762140462
667 667 c, t dbSNP:777026877
668 668 a, g dbSNP:773949476
672 672 c, t dbSNP:540789414
689 689 a, g dbSNP:73381183
691 691 c, t dbSNP:747549889
698 698 a, g dbSNP:776090604
706 706 a, c dbSNP:771720187
721 721 a, g dbSNP:745308839
722 722 a, g dbSNP:778497868
743 743 a, g dbSNP:148615168
745 745 c, g dbSNP:749085781
754 754 c, t dbSNP:777593933
755 755 a, g dbSNP:558400297
759 759 c, t dbSNP:143891160
769 769 a, g dbSNP:781053176
785 785 c, t dbSNP:377663087
796 796 c, t dbSNP:750500895
805 805 a, g dbSNP:765423615
811 811 c, t dbSNP:762060448
816 816 a, g dbSNP:753963673
827 827 a, c dbSNP:764540833
829 829 c, t dbSNP:761063801
830 830 c, g, t dbSNP:373485369
834 834 c, t dbSNP:759177221
834 834 -, t dbSNP:755954952
835 835 a, g dbSNP:369219030
837 837 a, g dbSNP:770529881
842 842 a, g dbSNP:533871548
843 843 c, g, t dbSNP:539421564
869 869 c, g dbSNP:188040791
872 872 c, t dbSNP:755438611
900 900 a, g dbSNP:5745646
902 902 a, g dbSNP:780660807
952 952 a, g dbSNP:555912845
956 956 g, t dbSNP:756801479
1015 1015 a, c dbSNP:536343283
1031 1031 c, t dbSNP:5745647
1033 1033 c, t dbSNP:373236095
1037 1037 a, g dbSNP:140804004
1088 1088 c, g dbSNP:5745648
1109 1109 a, g dbSNP:751170173
1113 1113 a, c dbSNP:112061786
1132 1132 a, g dbSNP:5745649
1134 1134 a, t dbSNP:763867460
1139 1139 -, c dbSNP:748968245
1185 1185 a, g dbSNP:5745650
1198 1198 a, c dbSNP:532069662
1220 1220 a, c dbSNP:758004208
1234 1234 a, c dbSNP:569229562
1246 1246 c, t dbSNP:190770462
1280 1280 c, g dbSNP:143596094
1310 1310 a, c dbSNP:556972476
1312 1312 a, t dbSNP:560340242
1317 1317 c, t dbSNP:540351923
1382 1382 -, acc dbSNP:373089454
1385 1385 -, tgg dbSNP:5745651
1387 1387 -, tgg dbSNP:200919155
1404 1404 -, atgtctatgg dbSNP:199531894
1405 1405 ggc, tgtctatgg dbSNP:71296588
1405 1405 -, tgtctat dbSNP:373999040
1405 1405 g, t dbSNP:112205118
1407 1407 -, tctat dbSNP:767377232
1407 1407 c, g, t dbSNP:55858258
1407 1407 -, t dbSNP:548603890
1408 1408 -, ctatgg dbSNP:148691571
1409 1409 -, tatgg dbSNP:532028995
1411 1411 -, tgtctat dbSNP:774071022
1413 1413 -, c dbSNP:11404675
1413 1413 c, g dbSNP:111441846
1467 1467 a, g dbSNP:564774096
1485 1485 c, g dbSNP:774830571
1542 1542 a, c dbSNP:544882472
1574 1574 c, t dbSNP:5745652
1596 1596 a, c dbSNP:556116025
1650 1650 a, g, t dbSNP:542218822
1652 1652 a, g dbSNP:5745653
1664 1664 c, g dbSNP:769835541
1683 1683 -, c dbSNP:377519841
1684 1684 g, t dbSNP:531037636
1685 1685 a, g dbSNP:373454718
1686 1686 c, t dbSNP:374476384
1712 1712 a, c dbSNP:5745654
1754 1754 a, g dbSNP:553868611
1759 1759 c, t dbSNP:10238283
1775 1775 a, g dbSNP:571847057
1815 1815 a, g dbSNP:745742385
1832 1832 a, g dbSNP:558435009
1854 1854 c, t dbSNP:368689212
1882 1882 c, t dbSNP:776755908
1942 1942 a, g dbSNP:538518912
1961 1961 c, g dbSNP:568709244
1989 1989 a, c dbSNP:569407574
2006 2006 g, t dbSNP:781139557

Target ORF information:

RefSeq Version NM_001010934
Organism Homo sapiens (human)
Definition Homo sapiens hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001010934

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGGTAAA TAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu25775D
Sequence Information ORF Nucleotide Sequence (Length: 2187bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product hepatocyte growth factor isoform 1 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU533235.1, M29145.1, M73240.1 and BM153501.1. This sequence is a reference standard in the RefSeqGene project. On Feb 3, 2005 this sequence version replaced gi:33859834. Summary: Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (1) encodes the longest isoform (1). To date, experimental evidence for cleavage of the proprotein into two mature chains has been shown only for isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK291674.1, X16323.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA GAGTTATCGA
GGTCTCATGG ATCATACAGA ATCAGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCA
CACCGGCACA AATTCTTGCC TGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC
CGCAATCCCG ATGGCCAGCC GAGGCCATGG TGCTATACTC TTGACCCTCA CACCCGCTGG
GAGTACTGTG CAATTAAAAC ATGCGCTGAC AATACTATGA ATGACACTGA TGTTCCTTTG
GAAACAACTG AATGCATCCA AGGTCAAGGA GAAGGCTACA GGGGCACTGT CAATACCATT
TGGAATGGAA TTCCATGTCA GCGTTGGGAT TCTCAGTATC CTCACGAGCA TGACATGACT
CCTGAAAATT TCAAGTGCAA GGACCTACGA GAAAATTACT GCCGAAATCC AGATGGGTCT
GAATCACCCT GGTGTTTTAC CACTGATCCA AACATCCGAG TTGGCTACTG CTCCCAAATT
CCAAACTGTG ATATGTCACA TGGACAAGAT TGTTATCGTG GGAATGGCAA AAATTATATG
GGCAACTTAT CCCAAACAAG ATCTGGACTA ACATGTTCAA TGTGGGACAA GAACATGGAA
GACTTACATC GTCATATCTT CTGGGAACCA GATGCAAGTA AGCTGAATGA GAATTACTGC
CGAAATCCAG ATGATGATGC TCATGGACCC TGGTGCTACA CGGGAAATCC ACTCATTCCT
TGGGATTATT GCCCTATTTC TCGTTGTGAA GGTGATACCA CACCTACAAT AGTCAATTTA
GACCATCCCG TAATATCTTG TGCCAAAACG AAACAATTGC GAGTTGTAAA TGGGATTCCA
ACACGAACAA ACATAGGATG GATGGTTAGT TTGAGATACA GAAATAAACA TATCTGCGGA
GGATCATTGA TAAAGGAGAG TTGGGTTCTT ACTGCACGAC AGTGTTTCCC TTCTCGAGAC
TTGAAAGATT ATGAAGCTTG GCTTGGAATT CATGATGTCC ACGGAAGAGG AGATGAGAAA
TGCAAACAGG TTCTCAATGT TTCCCAGCTG GTATATGGCC CTGAAGGATC AGATCTGGTT
TTAATGAAGC TTGCCAGGCC TGCTGTCCTG GATGATTTTG TTAGTACGAT TGATTTACCT
AATTATGGAT GCACAATTCC TGAAAAGACC AGTTGCAGTG TTTATGGCTG GGGCTACACT
GGATTGATCA ACTATGATGG CCTATTACGA GTGGCACATC TCTATATAAT GGGAAATGAG
AAATGCAGCC AGCATCATCG AGGGAAGGTG ACTCTGAATG AGTCTGAAAT ATGTGCTGGG
GCTGAAAAGA TTGGATCAGG ACCATGTGAG GGGGATTATG GTGGCCCACT TGTTTGTGAG
CAACATAAAA TGAGAATGGT TCTTGGTGTC ATTGTTCCTG GTCGTGGATG TGCCATTCCA
AATCGTCCTG GTATTTTTGT CCGAGTAGCA TATTATGCAA AATGGATACA CAAAATTATT
TTAACATATA AGGTACCACA GTCATAG
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_000592.3
CDS166..2352
Misc Feature(1)58..60(+)
Misc Feature(2)259..261(+)
Misc Feature(3)280..531(+)
Misc Feature(4)343..384(+)
Misc Feature(5)541..786(+)
Misc Feature(6)574..576(+)
Misc Feature(7)619..759(+)
Misc Feature(8)787..1032(+)
Misc Feature(9)823..825(+)
Misc Feature(10)868..1005(+)
Misc Feature(11)1069..1317(+)
Misc Feature(12)1105..1107(+)
Misc Feature(13)1150..1287(+)
Misc Feature(14)1327..1575(+)
Misc Feature(15)1363..1365(+)
Misc Feature(16)1408..1548(+)
Misc Feature(17)1435..1437(+)
Misc Feature(18)1435..1437(+)
Misc Feature(19)1645..2313(+)
Misc Feature(20)1645..1647(+)
Misc Feature(21)1645..1647(+)
Misc Feature(22)1645..1647(+)
Misc Feature(23)1648..2322(+)
Misc Feature(24)1648..1650(+)
Misc Feature(25)1765..2184(+)
Misc Feature(26)2164..2247(+)
Exon (1)1..253
Gene:HGF
Gene Synonym:
Exon (2)254..419
Gene:HGF
Gene Synonym:
Exon (3)420..532
Gene:HGF
Gene Synonym:
Exon (4)533..647
Gene:HGF
Gene Synonym:
Exon (5)648..790
Gene:HGF
Gene Synonym:
Exon (6)791..911
Gene:HGF
Gene Synonym:
Exon (7)912..1030
Gene:HGF
Gene Synonym:
Exon (8)1031..1205
Gene:HGF
Gene Synonym:
Exon (9)1206..1333
Gene:HGF
Gene Synonym:
Exon (10)1334..1436
Gene:HGF
Gene Synonym:
Exon (11)1437..1570
Gene:HGF
Gene Synonym:
Exon (12)1571..1609
Gene:HGF
Gene Synonym:
Exon (13)1610..1706
Gene:HGF
Gene Synonym:
Exon (14)1707..1781
Gene:HGF
Gene Synonym:
Exon (15)1782..1922
Gene:HGF
Gene Synonym:
Exon (16)1923..2029
Gene:HGF
Gene Synonym:
Exon (17)2030..2175
Gene:HGF
Gene Synonym:
Exon (18)2176..2805
Gene:HGF
Gene Synonym:
Translation
Position Chain Variation Link
24 24 c, g dbSNP:371718041
25 25 a, g dbSNP:545903754
32 32 c, t dbSNP:577719518
66 66 a, c dbSNP:76382276
72 72 a, c dbSNP:5745609
77 77 c, g dbSNP:575483475
81 81 a, g dbSNP:555377705
122 122 a, g dbSNP:758149409
125 125 a, c dbSNP:768292342
126 126 a, t dbSNP:28719812
149 149 g, t dbSNP:779801717
151 151 c, t dbSNP:370842395
152 152 c, g dbSNP:201598927
169 169 c, t dbSNP:375901177
171 171 g, t dbSNP:745851853
173 173 a, t dbSNP:778667611
192 192 c, t dbSNP:372751973
213 213 c, t dbSNP:148935472
217 217 c, t dbSNP:752758599
222 222 c, t dbSNP:781631751
223 223 a, c dbSNP:755241254
227 227 c, t dbSNP:370490787
238 238 -, gccatc dbSNP:764462723
257 257 a, g dbSNP:747253986
269 269 a, g, t dbSNP:758790721
270 270 a, g dbSNP:750744461
271 271 a, g dbSNP:765830204
272 272 -, gaa dbSNP:771230803
286 286 a, g dbSNP:376573289
293 293 a, c, g dbSNP:763623534
294 294 a, g dbSNP:17855203
298 298 a, t dbSNP:760130608
302 302 c, t dbSNP:150267054
311 311 a, c dbSNP:368094683
314 314 c, t dbSNP:759442724
321 321 a, g dbSNP:774007780
323 323 c, t dbSNP:746355265
339 339 a, g dbSNP:749210341
347 347 a, c, t dbSNP:142659098
348 348 c, t dbSNP:147718886
364 364 a, g dbSNP:780386537
381 381 c, t dbSNP:372588506
384 384 a, t dbSNP:746293406
394 394 a, g dbSNP:765051974
403 403 c, t dbSNP:757576097
408 408 a, g dbSNP:571028390
411 411 c, g dbSNP:778196890
423 423 c, t dbSNP:370202240
435 435 c, t dbSNP:145598174
437 437 a, g dbSNP:771300057
443 443 c, g dbSNP:189052337
450 450 a, g dbSNP:778319588
462 462 c, t dbSNP:368633795
471 471 c, t dbSNP:752305781
475 475 a, g dbSNP:780541101
497 497 a, t dbSNP:754474625
498 498 a, g dbSNP:5745635
500 500 g, t dbSNP:766059183
512 512 a, t dbSNP:151170133
519 519 c, t dbSNP:769553578
521 521 a, t dbSNP:750225776
537 537 c, t dbSNP:537365064
538 538 a, g dbSNP:781690781
552 552 a, c dbSNP:754448554
554 554 c, t dbSNP:141389863
557 557 a, g dbSNP:779906703
560 560 a, g dbSNP:758097772
566 566 a, g dbSNP:750282888
576 576 a, g dbSNP:765155428
585 585 a, g dbSNP:757026543
609 609 -, a dbSNP:771780436
611 611 a, g dbSNP:753731630
615 615 a, g dbSNP:764026915
623 623 g, t dbSNP:17566
636 636 a, g dbSNP:142045938
639 639 c, t dbSNP:367553438
648 648 c, t dbSNP:762973783
659 659 c, t dbSNP:370760302
660 660 a, g dbSNP:137853235
662 662 a, g dbSNP:373660832
665 665 a, g dbSNP:762140462
667 667 c, t dbSNP:777026877
668 668 a, g dbSNP:773949476
672 672 c, t dbSNP:540789414
689 689 a, g dbSNP:73381183
691 691 c, t dbSNP:747549889
698 698 a, g dbSNP:776090604
706 706 a, c dbSNP:771720187
721 721 a, g dbSNP:745308839
722 722 a, g dbSNP:778497868
743 743 a, g dbSNP:148615168
745 745 c, g dbSNP:749085781
754 754 c, t dbSNP:777593933
755 755 a, g dbSNP:558400297
759 759 c, t dbSNP:143891160
769 769 a, g dbSNP:781053176
785 785 c, t dbSNP:377663087
803 803 c, g dbSNP:144136982
824 824 a, g dbSNP:372600489
847 847 g, t dbSNP:139457161
864 864 a, g dbSNP:754102922
876 876 c, t dbSNP:5745666
880 880 a, g dbSNP:761186765
882 882 a, g dbSNP:753099990
884 884 c, t dbSNP:768190212
886 886 c, t dbSNP:760088738
889 889 c, t dbSNP:202185530
890 890 a, g dbSNP:200559590
894 894 c, t dbSNP:762316469
903 903 a, g dbSNP:573737350
906 906 c, t dbSNP:772926410
918 918 c, t dbSNP:755250749
919 919 a, g dbSNP:752123971
923 923 a, g dbSNP:559265540
926 926 c, g, t dbSNP:560215969
927 927 c, t dbSNP:772684432
938 938 a, c dbSNP:764905780
940 940 c, t dbSNP:761481749
942 942 c, t dbSNP:776214617
945 945 c, t dbSNP:375177081
947 947 a, g dbSNP:768451705
954 954 c, t dbSNP:147312555
959 959 c, g dbSNP:775564237
965 965 c, t dbSNP:771889279
967 967 a, g dbSNP:749434892
996 996 a, c dbSNP:777970277
997 997 a, t dbSNP:754578668
1000 1000 c, t dbSNP:756224070
1001 1001 g, t dbSNP:371502313
1014 1014 g, t dbSNP:533086523
1015 1015 a, g dbSNP:755376784
1019 1019 c, t dbSNP:751870029
1025 1025 c, t dbSNP:766971992
1026 1026 a, g dbSNP:368002816
1029 1029 c, t dbSNP:374136304
1030 1030 a, g dbSNP:764746019
1035 1035 c, t dbSNP:778286006
1037 1037 a, c dbSNP:755584740
1042 1042 a, g dbSNP:747693036
1043 1043 a, t dbSNP:780768372
1045 1045 a, t dbSNP:754659494
1048 1048 c, g dbSNP:367811213
1050 1050 a, c, t dbSNP:141751290
1053 1053 a, t dbSNP:750329013
1058 1058 g, t dbSNP:775912518
1061 1061 c, t dbSNP:760721822
1070 1070 c, t dbSNP:770152785
1075 1075 a, g dbSNP:5745687
1082 1082 a, t dbSNP:767766226
1085 1085 a, c dbSNP:759784966
1086 1086 a, g dbSNP:774508303
1097 1097 a, g dbSNP:771299734
1099 1099 a, g dbSNP:749600508
1101 1101 c, t dbSNP:773660386
1105 1105 a, t dbSNP:781437504
1113 1113 g, t dbSNP:770323794
1122 1122 c, t dbSNP:747664002
1125 1125 c, t dbSNP:780821031
1147 1147 c, t dbSNP:754502188
1148 1148 a, g, t dbSNP:374484762
1149 1149 c, t dbSNP:779783865
1153 1153 g, t dbSNP:5745688
1154 1154 a, t dbSNP:750217931
1164 1164 c, t dbSNP:569559437
1170 1170 c, t dbSNP:757186641
1171 1171 a, g dbSNP:549654942
1173 1173 a, g dbSNP:148714837
1194 1194 c, t dbSNP:759555986
1200 1200 a, g dbSNP:751786738
1202 1202 a, g dbSNP:766585116
1208 1208 a, g dbSNP:763206701
1213 1213 a, c dbSNP:373442319
1214 1214 a, g dbSNP:750575553
1215 1215 a, g dbSNP:547103298
1238 1238 a, g dbSNP:184858341
1245 1245 c, t dbSNP:765660542
1248 1248 a, t dbSNP:762012189
1256 1256 a, g dbSNP:5745703
1263 1263 c, t dbSNP:777341007
1279 1279 a, t dbSNP:769270957
1293 1293 c, t dbSNP:112557787
1300 1300 a, c dbSNP:200948659
1303 1303 a, g dbSNP:771441595
1315 1315 a, g dbSNP:745555932
1316 1316 a, g dbSNP:778372935
1318 1318 a, g dbSNP:770765351
1324 1324 a, c dbSNP:749078919
1325 1325 a, t dbSNP:777748021
1342 1342 c, t dbSNP:758567520
1343 1343 a, g dbSNP:745919057
1344 1344 c, t dbSNP:377036882
1352 1352 c, g dbSNP:745854997
1359 1359 c, t dbSNP:754071169
1366 1366 a, g dbSNP:764574926
1368 1368 c, t dbSNP:756489380
1395 1395 a, g dbSNP:373298029
1427 1427 a, c dbSNP:767907135
1435 1435 c, t dbSNP:759145490
1440 1440 c, t dbSNP:755416276
1456 1456 c, g dbSNP:61735997
1459 1459 a, g dbSNP:752052736
1489 1489 a, c dbSNP:765902946
1507 1507 c, g dbSNP:762379250
1524 1524 a, c dbSNP:750085970
1526 1526 c, t dbSNP:764869064
1527 1527 a, g dbSNP:761711142
1531 1531 a, c dbSNP:776270506
1539 1539 c, t dbSNP:566128293
1545 1545 c, t dbSNP:760563094
1553 1553 a, g dbSNP:775433097
1561 1561 a, t dbSNP:141487191
1568 1568 a, g dbSNP:148293411
1587 1587 a, g dbSNP:764922029
1609 1609 c, t dbSNP:761396857
1614 1614 c, t dbSNP:375872396
1615 1615 a, g, t dbSNP:571102402
1617 1617 a, g dbSNP:759566751
1619 1619 c, t dbSNP:397516446
1621 1621 c, t dbSNP:774389172
1631 1631 a, g dbSNP:371520373
1635 1635 a, g, t dbSNP:372075114
1642 1642 c, t dbSNP:768896096
1669 1669 -, c dbSNP:748881407
1672 1672 a, g dbSNP:747426081
1673 1673 c, g dbSNP:775857995
1678 1678 a, g dbSNP:772388735
1686 1686 a, g dbSNP:200549396
1689 1689 g, t dbSNP:746380080
1693 1693 a, g dbSNP:779336001
1697 1697 c, t dbSNP:756776269
1702 1702 c, t dbSNP:572203404
1718 1718 c, t dbSNP:776754331
1728 1728 a, t dbSNP:764035851
1743 1743 c, g dbSNP:143262934
1746 1746 c, t dbSNP:775625219
1755 1755 a, t dbSNP:772441910
1759 1759 a, g dbSNP:759881207
1762 1762 c, t dbSNP:527951814
1763 1763 a, g, t dbSNP:771437724
1764 1764 a, g dbSNP:748796647
1779 1779 a, t dbSNP:368519009
1792 1792 g, t dbSNP:769438908
1814 1814 a, t dbSNP:192078086
1817 1817 a, c, g dbSNP:752861058
1827 1827 c, t dbSNP:148098479
1828 1828 a, g dbSNP:779933934
1837 1837 a, g dbSNP:370757602
1839 1839 c, g, t dbSNP:144086354
1840 1840 a, g dbSNP:778704152
1850 1850 a, g dbSNP:559990458
1852 1852 c, g dbSNP:376043671
1863 1863 c, t dbSNP:752743857
1869 1869 c, t dbSNP:138548003
1873 1873 c, t dbSNP:759776706
1878 1878 a, g dbSNP:751689885
1880 1880 a, g dbSNP:766769838
1890 1890 a, g dbSNP:763167149
1896 1896 a, g dbSNP:773824390
1910 1910 c, t dbSNP:765831517
1911 1911 a, g dbSNP:201395847
1929 1929 g, t dbSNP:531850775
1930 1930 a, g dbSNP:538415452
1938 1938 c, t dbSNP:199516810
1941 1941 c, t dbSNP:754944443
1952 1952 c, t dbSNP:751819844
1953 1953 a, g dbSNP:766493585
1958 1958 a, g dbSNP:758862083
1979 1979 c, t dbSNP:147075806
1981 1981 a, g dbSNP:765890500
1983 1983 c, t dbSNP:762445545
1994 1994 a, c dbSNP:777216192
1997 1997 c, g dbSNP:141774517
2003 2003 a, g dbSNP:760093179
2013 2013 c, g dbSNP:775281878
2018 2018 g, t dbSNP:771594786
2022 2022 c, t dbSNP:200374420
2024 2024 c, t dbSNP:774153258
2027 2027 c, g dbSNP:770672389
2031 2031 a, g dbSNP:376395424
2032 2032 a, g dbSNP:371826587
2034 2034 c, t dbSNP:755575139
2037 2037 a, c, t dbSNP:373545882
2045 2045 a, g dbSNP:770723689
2049 2049 a, g dbSNP:762721825
2050 2050 c, t dbSNP:773198515
2053 2053 c, t dbSNP:769585774
2054 2054 a, g dbSNP:747158538
2056 2056 a, g dbSNP:145494248
2063 2063 a, c, g dbSNP:746110664
2065 2065 a, c dbSNP:779267373
2069 2069 a, g dbSNP:565056780
2071 2071 a, g dbSNP:754226152
2082 2082 c, t dbSNP:778395987
2084 2084 a, g dbSNP:756602776
2099 2099 a, g dbSNP:202215700
2104 2104 c, t dbSNP:545249104
2107 2107 a, g dbSNP:150109563
2109 2109 a, g dbSNP:369418750
2119 2119 c, t dbSNP:751270979
2130 2130 c, t dbSNP:766007676
2144 2144 c, g, t dbSNP:772967560
2146 2146 a, g dbSNP:769580628
2155 2155 a, t dbSNP:761664295
2159 2159 g, t dbSNP:776576073
2172 2172 c, t dbSNP:772105726
2177 2177 a, g dbSNP:770114698
2184 2184 c, t dbSNP:367800414
2190 2190 c, t dbSNP:781586687
2193 2193 a, g dbSNP:755450918
2197 2197 c, g dbSNP:747463316
2203 2203 a, g dbSNP:544960907
2206 2206 c, g dbSNP:779568330
2224 2224 g, t dbSNP:757849670
2235 2235 a, c dbSNP:750104031
2247 2247 a, t dbSNP:563705580
2249 2249 a, g dbSNP:543827901
2260 2260 a, g dbSNP:372366408
2270 2270 a, g dbSNP:763933267
2275 2275 a, g dbSNP:760728215
2279 2279 g, t dbSNP:775503666
2287 2287 c, t dbSNP:201768315
2288 2288 a, g dbSNP:763072412
2295 2295 a, c, g dbSNP:267601578
2301 2301 c, t dbSNP:770025635
2304 2304 a, t dbSNP:764730119
2331 2331 a, g dbSNP:561484014
2334 2334 c, t dbSNP:777071855
2344 2344 c, t dbSNP:140790665
2348 2348 a, c dbSNP:747516423
2350 2350 c, t dbSNP:780528260
2352 2352 a, g dbSNP:757972650
2358 2358 a, g dbSNP:745356078
2360 2360 -, aagt dbSNP:779001317
2362 2362 a, g dbSNP:778483338
2374 2374 c, t dbSNP:756957205
2381 2381 a, c dbSNP:753392862
2383 2383 a, g dbSNP:777717109
2388 2388 a, g dbSNP:767332482
2392 2392 a, c, t dbSNP:377185441
2397 2397 c, t dbSNP:767556298
2400 2400 a, c, g dbSNP:750490813
2401 2401 c, t dbSNP:765177187
2409 2409 g, t dbSNP:542402530
2418 2418 a, g dbSNP:573406409
2454 2454 -, aact dbSNP:374789745
2520 2520 c, t dbSNP:531222230
2573 2573 a, g dbSNP:544879896
2576 2576 a, g dbSNP:539702697
2611 2611 a, c dbSNP:777313117
2618 2618 -, ca dbSNP:763357973
2618 2618 c, g dbSNP:577625800
2624 2624 a, t dbSNP:758013381
2654 2654 a, t dbSNP:186434043
2666 2666 -, taat dbSNP:545984462
2705 2705 a, g dbSNP:537468151
2739 2739 c, t dbSNP:568550366
2748 2748 a, t dbSNP:182057547
2766 2766 a, c dbSNP:535633453
2804 2804 c, g dbSNP:778665027

Target ORF information:

RefSeq Version NM_000601
Organism Homo sapiens (human)
Definition Homo sapiens hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_000601

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA GAGTTATCGA
GGTCTCATGG ATCATACAGA ATCAGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCA
CACCGGCACA AATTCTTGCC TGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC
CGCAATCCCG ATGGCCAGCC GAGGCCATGG TGCTATACTC TTGACCCTCA CACCCGCTGG
GAGTACTGTG CAATTAAAAC ATGCGCTGAC AATACTATGA ATGACACTGA TGTTCCTTTG
GAAACAACTG AATGCATCCA AGGTCAAGGA GAAGGCTACA GGGGCACTGT CAATACCATT
TGGAATGGAA TTCCATGTCA GCGTTGGGAT TCTCAGTATC CTCACGAGCA TGACATGACT
CCTGAAAATT TCAAGTGCAA GGACCTACGA GAAAATTACT GCCGAAATCC AGATGGGTCT
GAATCACCCT GGTGTTTTAC CACTGATCCA AACATCCGAG TTGGCTACTG CTCCCAAATT
CCAAACTGTG ATATGTCACA TGGACAAGAT TGTTATCGTG GGAATGGCAA AAATTATATG
GGCAACTTAT CCCAAACAAG ATCTGGACTA ACATGTTCAA TGTGGGACAA GAACATGGAA
GACTTACATC GTCATATCTT CTGGGAACCA GATGCAAGTA AGCTGAATGA GAATTACTGC
CGAAATCCAG ATGATGATGC TCATGGACCC TGGTGCTACA CGGGAAATCC ACTCATTCCT
TGGGATTATT GCCCTATTTC TCGTTGTGAA GGTGATACCA CACCTACAAT AGTCAATTTA
GACCATCCCG TAATATCTTG TGCCAAAACG AAACAATTGC GAGTTGTAAA TGGGATTCCA
ACACGAACAA ACATAGGATG GATGGTTAGT TTGAGATACA GAAATAAACA TATCTGCGGA
GGATCATTGA TAAAGGAGAG TTGGGTTCTT ACTGCACGAC AGTGTTTCCC TTCTCGAGAC
TTGAAAGATT ATGAAGCTTG GCTTGGAATT CATGATGTCC ACGGAAGAGG AGATGAGAAA
TGCAAACAGG TTCTCAATGT TTCCCAGCTG GTATATGGCC CTGAAGGATC AGATCTGGTT
TTAATGAAGC TTGCCAGGCC TGCTGTCCTG GATGATTTTG TTAGTACGAT TGATTTACCT
AATTATGGAT GCACAATTCC TGAAAAGACC AGTTGCAGTG TTTATGGCTG GGGCTACACT
GGATTGATCA ACTATGATGG CCTATTACGA GTGGCACATC TCTATATAAT GGGAAATGAG
AAATGCAGCC AGCATCATCG AGGGAAGGTG ACTCTGAATG AGTCTGAAAT ATGTGCTGGG
GCTGAAAAGA TTGGATCAGG ACCATGTGAG GGGGATTATG GTGGCCCACT TGTTTGTGAG
CAACATAAAA TGAGAATGGT TCTTGGTGTC ATTGTTCCTG GTCGTGGATG TGCCATTCCA
AATCGTCCTG GTATTTTTGT CCGAGTAGCA TATTATGCAA AATGGATACA CAAAATTATT
TTAACATATA AGGTACCACA GTCATAG

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu25877D
Sequence Information ORF Nucleotide Sequence (Length: 2172bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product hepatocyte growth factor isoform 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU533235.1, M73240.1 and BM153501.1. Summary: Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (3) lacks an in-frame segment in the middle coding region, compared to variant 1. The resulting isoform 3 lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M73240.1, AK313694.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTATCGGG GTAAAGACCT ACAGGAAAAC TACTGTCGAA ATCCTCGAGG GGAAGAAGGG
GGACCCTGGT GTTTCACAAG CAATCCAGAG GTACGCTACG AAGTCTGTGA CATTCCTCAG
TGTTCAGAAG TTGAATGCAT GACCTGCAAT GGGGAGAGTT ATCGAGGTCT CATGGATCAT
ACAGAATCAG GCAAGATTTG TCAGCGCTGG GATCATCAGA CACCACACCG GCACAAATTC
TTGCCTGAAA GATATCCCGA CAAGGGCTTT GATGATAATT ATTGCCGCAA TCCCGATGGC
CAGCCGAGGC CATGGTGCTA TACTCTTGAC CCTCACACCC GCTGGGAGTA CTGTGCAATT
AAAACATGCG CTGACAATAC TATGAATGAC ACTGATGTTC CTTTGGAAAC AACTGAATGC
ATCCAAGGTC AAGGAGAAGG CTACAGGGGC ACTGTCAATA CCATTTGGAA TGGAATTCCA
TGTCAGCGTT GGGATTCTCA GTATCCTCAC GAGCATGACA TGACTCCTGA AAATTTCAAG
TGCAAGGACC TACGAGAAAA TTACTGCCGA AATCCAGATG GGTCTGAATC ACCCTGGTGT
TTTACCACTG ATCCAAACAT CCGAGTTGGC TACTGCTCCC AAATTCCAAA CTGTGATATG
TCACATGGAC AAGATTGTTA TCGTGGGAAT GGCAAAAATT ATATGGGCAA CTTATCCCAA
ACAAGATCTG GACTAACATG TTCAATGTGG GACAAGAACA TGGAAGACTT ACATCGTCAT
ATCTTCTGGG AACCAGATGC AAGTAAGCTG AATGAGAATT ACTGCCGAAA TCCAGATGAT
GATGCTCATG GACCCTGGTG CTACACGGGA AATCCACTCA TTCCTTGGGA TTATTGCCCT
ATTTCTCGTT GTGAAGGTGA TACCACACCT ACAATAGTCA ATTTAGACCA TCCCGTAATA
TCTTGTGCCA AAACGAAACA ATTGCGAGTT GTAAATGGGA TTCCAACACG AACAAACATA
GGATGGATGG TTAGTTTGAG ATACAGAAAT AAACATATCT GCGGAGGATC ATTGATAAAG
GAGAGTTGGG TTCTTACTGC ACGACAGTGT TTCCCTTCTC GAGACTTGAA AGATTATGAA
GCTTGGCTTG GAATTCATGA TGTCCACGGA AGAGGAGATG AGAAATGCAA ACAGGTTCTC
AATGTTTCCC AGCTGGTATA TGGCCCTGAA GGATCAGATC TGGTTTTAAT GAAGCTTGCC
AGGCCTGCTG TCCTGGATGA TTTTGTTAGT ACGATTGATT TACCTAATTA TGGATGCACA
ATTCCTGAAA AGACCAGTTG CAGTGTTTAT GGCTGGGGCT ACACTGGATT GATCAACTAT
GATGGCCTAT TACGAGTGGC ACATCTCTAT ATAATGGGAA ATGAGAAATG CAGCCAGCAT
CATCGAGGGA AGGTGACTCT GAATGAGTCT GAAATATGTG CTGGGGCTGA AAAGATTGGA
TCAGGACCAT GTGAGGGGGA TTATGGTGGC CCACTTGTTT GTGAGCAACA TAAAATGAGA
ATGGTTCTTG GTGTCATTGT TCCTGGTCGT GGATGTGCCA TTCCAAATCG TCCTGGTATT
TTTGTCCGAG TAGCATATTA TGCAAAATGG ATACACAAAA TTATTTTAAC ATATAAGGTA
CCACAGTCATT AG
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001010932.1
CDS166..2337
Misc Feature(1)58..60(+)
Misc Feature(2)280..531(+)
Misc Feature(3)343..384(+)
Misc Feature(4)541..771(+)
Misc Feature(5)574..576(+)
Misc Feature(6)619..744(+)
Misc Feature(7)772..1017(+)
Misc Feature(8)808..810(+)
Misc Feature(9)853..990(+)
Misc Feature(10)1054..1302(+)
Misc Feature(11)1090..1092(+)
Misc Feature(12)1135..1272(+)
Misc Feature(13)1312..1560(+)
Misc Feature(14)1348..1350(+)
Misc Feature(15)1393..1533(+)
Misc Feature(16)1420..1422(+)
Misc Feature(17)1420..1422(+)
Misc Feature(18)1630..2298(+)
Misc Feature(19)1630..1632(+)
Misc Feature(20)1630..1632(+)
Misc Feature(21)1630..1632(+)
Misc Feature(22)1633..2307(+)
Misc Feature(23)1633..1635(+)
Misc Feature(24)1750..2169(+)
Misc Feature(25)2149..2232(+)
Exon (1)1..253
Gene:HGF
Gene Synonym:
Exon (2)254..419
Gene:HGF
Gene Synonym:
Exon (3)420..532
Gene:HGF
Gene Synonym:
Exon (4)533..647
Gene:HGF
Gene Synonym:
Exon (5)648..775
Gene:HGF
Gene Synonym:
Exon (6)776..896
Gene:HGF
Gene Synonym:
Exon (7)897..1015
Gene:HGF
Gene Synonym:
Exon (8)1016..1190
Gene:HGF
Gene Synonym:
Exon (9)1191..1318
Gene:HGF
Gene Synonym:
Exon (10)1319..1421
Gene:HGF
Gene Synonym:
Exon (11)1422..1555
Gene:HGF
Gene Synonym:
Exon (12)1556..1594
Gene:HGF
Gene Synonym:
Exon (13)1595..1691
Gene:HGF
Gene Synonym:
Exon (14)1692..1766
Gene:HGF
Gene Synonym:
Exon (15)1767..1907
Gene:HGF
Gene Synonym:
Exon (16)1908..2014
Gene:HGF
Gene Synonym:
Exon (17)2015..2160
Gene:HGF
Gene Synonym:
Exon (18)2161..2790
Gene:HGF
Gene Synonym:
Translation
Position Chain Variation Link
24 24 c, g dbSNP:371718041
25 25 a, g dbSNP:545903754
32 32 c, t dbSNP:577719518
66 66 a, c dbSNP:76382276
72 72 a, c dbSNP:5745609
77 77 c, g dbSNP:575483475
81 81 a, g dbSNP:555377705
122 122 a, g dbSNP:758149409
125 125 a, c dbSNP:768292342
126 126 a, t dbSNP:28719812
149 149 g, t dbSNP:779801717
151 151 c, t dbSNP:370842395
152 152 c, g dbSNP:201598927
169 169 c, t dbSNP:375901177
171 171 g, t dbSNP:745851853
173 173 a, t dbSNP:778667611
192 192 c, t dbSNP:372751973
213 213 c, t dbSNP:148935472
217 217 c, t dbSNP:752758599
222 222 c, t dbSNP:781631751
223 223 a, c dbSNP:755241254
227 227 c, t dbSNP:370490787
238 238 -, gccatc dbSNP:764462723
257 257 a, g dbSNP:747253986
269 269 a, g, t dbSNP:758790721
270 270 a, g dbSNP:750744461
271 271 a, g dbSNP:765830204
272 272 -, gaa dbSNP:771230803
286 286 a, g dbSNP:376573289
293 293 a, c, g dbSNP:763623534
294 294 a, g dbSNP:17855203
298 298 a, t dbSNP:760130608
302 302 c, t dbSNP:150267054
311 311 a, c dbSNP:368094683
314 314 c, t dbSNP:759442724
321 321 a, g dbSNP:774007780
323 323 c, t dbSNP:746355265
339 339 a, g dbSNP:749210341
347 347 a, c, t dbSNP:142659098
348 348 c, t dbSNP:147718886
364 364 a, g dbSNP:780386537
381 381 c, t dbSNP:372588506
384 384 a, t dbSNP:746293406
394 394 a, g dbSNP:765051974
403 403 c, t dbSNP:757576097
408 408 a, g dbSNP:571028390
411 411 c, g dbSNP:778196890
423 423 c, t dbSNP:370202240
435 435 c, t dbSNP:145598174
437 437 a, g dbSNP:771300057
443 443 c, g dbSNP:189052337
450 450 a, g dbSNP:778319588
462 462 c, t dbSNP:368633795
471 471 c, t dbSNP:752305781
475 475 a, g dbSNP:780541101
497 497 a, t dbSNP:754474625
498 498 a, g dbSNP:5745635
500 500 g, t dbSNP:766059183
512 512 a, t dbSNP:151170133
519 519 c, t dbSNP:769553578
521 521 a, t dbSNP:750225776
537 537 c, t dbSNP:537365064
538 538 a, g dbSNP:781690781
552 552 a, c dbSNP:754448554
554 554 c, t dbSNP:141389863
557 557 a, g dbSNP:779906703
560 560 a, g dbSNP:758097772
566 566 a, g dbSNP:750282888
576 576 a, g dbSNP:765155428
585 585 a, g dbSNP:757026543
609 609 -, a dbSNP:771780436
611 611 a, g dbSNP:753731630
615 615 a, g dbSNP:764026915
623 623 g, t dbSNP:17566
636 636 a, g dbSNP:142045938
639 639 c, t dbSNP:367553438
650 650 a, g dbSNP:762140462
652 652 c, t dbSNP:777026877
653 653 a, g dbSNP:773949476
657 657 c, t dbSNP:540789414
674 674 a, g dbSNP:73381183
676 676 c, t dbSNP:747549889
683 683 a, g dbSNP:776090604
691 691 a, c dbSNP:771720187
706 706 a, g dbSNP:745308839
707 707 a, g dbSNP:778497868
728 728 a, g dbSNP:148615168
730 730 c, g dbSNP:749085781
739 739 c, t dbSNP:777593933
740 740 a, g dbSNP:558400297
744 744 c, t dbSNP:143891160
754 754 a, g dbSNP:781053176
770 770 c, t dbSNP:377663087
788 788 c, g dbSNP:144136982
809 809 a, g dbSNP:372600489
832 832 g, t dbSNP:139457161
849 849 a, g dbSNP:754102922
861 861 c, t dbSNP:5745666
865 865 a, g dbSNP:761186765
867 867 a, g dbSNP:753099990
869 869 c, t dbSNP:768190212
871 871 c, t dbSNP:760088738
874 874 c, t dbSNP:202185530
875 875 a, g dbSNP:200559590
879 879 c, t dbSNP:762316469
888 888 a, g dbSNP:573737350
891 891 c, t dbSNP:772926410
903 903 c, t dbSNP:755250749
904 904 a, g dbSNP:752123971
908 908 a, g dbSNP:559265540
911 911 c, g, t dbSNP:560215969
912 912 c, t dbSNP:772684432
923 923 a, c dbSNP:764905780
925 925 c, t dbSNP:761481749
927 927 c, t dbSNP:776214617
930 930 c, t dbSNP:375177081
932 932 a, g dbSNP:768451705
939 939 c, t dbSNP:147312555
944 944 c, g dbSNP:775564237
950 950 c, t dbSNP:771889279
952 952 a, g dbSNP:749434892
981 981 a, c dbSNP:777970277
982 982 a, t dbSNP:754578668
985 985 c, t dbSNP:756224070
986 986 g, t dbSNP:371502313
999 999 g, t dbSNP:533086523
1000 1000 a, g dbSNP:755376784
1004 1004 c, t dbSNP:751870029
1010 1010 c, t dbSNP:766971992
1011 1011 a, g dbSNP:368002816
1014 1014 c, t dbSNP:374136304
1015 1015 a, g dbSNP:764746019
1020 1020 c, t dbSNP:778286006
1022 1022 a, c dbSNP:755584740
1027 1027 a, g dbSNP:747693036
1028 1028 a, t dbSNP:780768372
1030