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HLA-DOB major histocompatibility complex, class II, DO beta [Homo sapiens (human)]

Gene Symbol HLA-DOB
Entrez Gene ID 3112
Full Name major histocompatibility complex, class II, DO beta
Synonyms DOB
General protein information
Preferred Names
HLA class II histocompatibility antigen, DO beta chain
Names
HLA class II histocompatibility antigen, DO beta chain
MHC class II antigen DOB
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p21.3

Summary HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

600629

Disorder Html:





The following HLA-DOB gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HLA-DOB gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19686 NM_002120 Homo sapiens major histocompatibility complex, class II, DO beta (HLA-DOB), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $309

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu19686
Accession Version NM_002120.3
Sequence Information ORF Nucleotide Sequence (Length: 822bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product HLA class II histocompatibility antigen, DO beta chain precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM152694.1, BC006097.1 and BQ010608.1. This sequence is a reference standard in the RefSeqGene project. On Nov 24, 2006 this sequence version replaced gi:18641377. Summary: HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006097.1, AY645723.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGTTCTG GGTGGGTCCC CTGGGTGGTG GCTCTGCTAG TGAATCTGAC CCGACTGGAT 
TCCTCCATGA CTCAAGGCAC AGACTCTCCA GAAGATTTTG TGATTCAGGC AAAGGCTGAC
TGTTACTTCA CCAACGGGAC AGAAAAGGTG CAGTTTGTGG TCAGATTCAT CTTTAACTTG
GAGGAGTATG TACGTTTCGA CAGTGATGTG GGGATGTTTG TGGCATTGAC CAAGCTGGGG
CAGCCAGATG CTGAGCAGTG GAACAGCCGG CTGGATCTCT TGGAGAGGAG CAGACAGGCC
GTGGATGGGG TCTGTAGACA CAACTACAGG CTGGGCGCAC CCTTCACTGT GGGGAGAAAA
GTGCAACCAG AGGTGACAGT GTACCCAGAG AGGACCCCAC TCCTGCACCA GCATAATCTG
CTGCACTGCT CTGTGACAGG CTTCTATCCA GGGGATATCA AGATCAAGTG GTTCCTGAAT
GGGCAGGAGG AGAGAGCTGG GGTCATGTCC ACTGGCCCTA TCAGGAATGG AGACTGGACC
TTTCAGACTG TGGTGATGCT AGAAATGACT CCTGAACTTG GACATGTCTA CACCTGCCTT
GTCGATCACT CCAGCCTGCT GAGCCCTGTT TCTGTGGAGT GGAGAGCTCA GTCTGAATAT
TCTTGGAGAA AGATGCTGAG TGGCATTGCA GCCTTCCTAC TTGGGCTAAT CTTCCTTCTG
GTGGGAATCG TCATCCAGCT AAGGGCTCAG AAAGGATATG TGAGGACGCA GATGTCTGGT
AATGAGGTCT CAAGAGCTGT TCTGCTCCCT CAGTCATGCT AA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_002111.1
CDS98..919
Misc Feature(1)176..457(+)
Misc Feature(2)212..436(+)
Misc Feature(3)458..739(+)
Misc Feature(4)464..745(+)
Misc Feature(5)740..769(+)
Misc Feature(6)770..832(+)
Exon (1)1..188
Gene:HLA-DOB
Gene Synonym:
Exon (2)189..458
Gene:HLA-DOB
Gene Synonym:
Exon (3)459..740
Gene:HLA-DOB
Gene Synonym:
Exon (4)741..851
Gene:HLA-DOB
Gene Synonym:
Exon (5)852..883
Gene:HLA-DOB
Gene Synonym:
Exon (6)884..1372
Gene:HLA-DOB
Gene Synonym:
Translation
Position Chain Variation Link
43 43 a, g dbSNP:2071469
46 46 c, t dbSNP:773037752
57 57 a, g dbSNP:771810386
58 58 a, g dbSNP:761430311
73 73 a, g dbSNP:2071470
85 85 c, t dbSNP:549419981
88 88 c, t dbSNP:544096527
94 94 c, t dbSNP:780408640
98 98 a, g dbSNP:770246200
109 109 a, g dbSNP:746190236
116 116 c, g dbSNP:781428784
119 119 c, t dbSNP:757426122
142 142 c, t dbSNP:751653725
147 147 c, t dbSNP:777764420
150 150 a, g dbSNP:2071554
151 151 a, c, t dbSNP:112887968
154 154 a, g dbSNP:753872785
162 162 c, t dbSNP:555199989
163 163 c, t dbSNP:543275553
164 164 a, g dbSNP:567290998
171 171 a, g dbSNP:767290583
175 175 a, c dbSNP:761487088
180 180 a, g dbSNP:774137652
181 181 c, t dbSNP:2071471
193 193 c, t dbSNP:7383287
194 194 c, t dbSNP:193195994
217 217 c, t dbSNP:767482695
229 229 c, t dbSNP:150439063
232 232 c, t dbSNP:374787208
233 233 a, g dbSNP:763626572
234 234 a, g dbSNP:762558634
244 244 c, g dbSNP:201748352
251 251 c, t dbSNP:774818152
253 253 c, t dbSNP:765791205
254 254 c, g dbSNP:759970888
262 262 a, g dbSNP:777119128
275 275 c, t dbSNP:771344909
278 278 a, g dbSNP:747354293
283 283 a, g dbSNP:141308106
290 290 c, t dbSNP:147835586
291 291 a, g dbSNP:144504518
295 295 c, t dbSNP:778791940
296 296 a, g dbSNP:755078086
317 317 a, g dbSNP:745854662
320 320 g, t dbSNP:781250640
328 328 a, c dbSNP:757155412
331 331 a, g dbSNP:751362778
344 344 g, t dbSNP:763832575
346 346 c, t dbSNP:757934639
348 348 c, t dbSNP:752260801
350 350 a, g dbSNP:764720977
353 353 a, c dbSNP:142229094
355 355 g, t dbSNP:754394327
357 357 g, t dbSNP:533842746
365 365 a, c, g, t dbSNP:756168942
366 366 a, g, t dbSNP:762003876
367 367 a, g, t dbSNP:370365246
375 375 a, t dbSNP:749345559
376 376 a, c dbSNP:781313228
382 382 a, g dbSNP:2239697
383 383 a, g dbSNP:746837449
392 392 c, t dbSNP:371721345
394 394 g, t dbSNP:758209124
396 396 c, t dbSNP:752350484
397 397 c, t dbSNP:778608492
398 398 a, g dbSNP:754523851
402 402 a, t dbSNP:557915642
405 405 g, t dbSNP:144814623
406 406 a, g dbSNP:781287067
416 416 a, c dbSNP:761103358
421 421 c, t dbSNP:148406921
424 424 c, g dbSNP:144487320
430 430 c, g dbSNP:767826958
433 433 c, t dbSNP:370963057
443 443 a, g dbSNP:774474315
445 445 g, t dbSNP:768956535
446 446 c, g dbSNP:763150534
447 447 c, g, t dbSNP:771000993
452 452 a, g dbSNP:746989562
453 453 a, g dbSNP:143319753
461 461 c, t dbSNP:747906961
470 470 a, g dbSNP:774065224
478 478 a, g dbSNP:768435116
482 482 c, t dbSNP:201263357
501 501 c, t dbSNP:779654674
503 503 c, t dbSNP:755617026
507 507 a, g dbSNP:745339672
511 511 a, t dbSNP:747832616
512 512 a, c dbSNP:781570264
519 519 c, t dbSNP:757726803
521 521 c, t dbSNP:751980029
533 533 a, g, t dbSNP:775119133
539 539 a, t dbSNP:758826813
542 542 c, t dbSNP:377749967
549 549 a, g dbSNP:267600978
552 552 a, t dbSNP:765526066
558 558 a, g dbSNP:759567704
559 559 a, g dbSNP:202214628
560 560 a, g dbSNP:575352220
561 561 c, t dbSNP:767358961
567 567 a, g, t dbSNP:146350950
579 579 a, g dbSNP:768488389
582 582 a, g dbSNP:749047487
586 586 g, t dbSNP:775152204
587 587 c, g dbSNP:142148406
591 591 g, t dbSNP:745386112
604 604 a, g dbSNP:776204344
609 609 -, c dbSNP:768479460
609 609 c, t dbSNP:772435801
612 612 -, g dbSNP:375745436
617 617 a, t dbSNP:567808719
618 618 c, t dbSNP:767194638
624 624 a, g dbSNP:201198783
628 628 a, g dbSNP:778402817
648 648 c, t dbSNP:758875780
651 651 c, t dbSNP:753073345
654 654 c, t dbSNP:779027285
655 655 a, g, t dbSNP:754000934
664 664 a, g dbSNP:766537004
676 676 g, t dbSNP:760721068
679 679 a, t dbSNP:549375506
682 682 c, t dbSNP:763975406
684 684 a, t dbSNP:762756450
688 688 c, t dbSNP:17501267
689 689 -, ac dbSNP:775211133
689 689 a, c, g dbSNP:113873432
691 691 c, t dbSNP:150977248
695 695 a, c dbSNP:769338306
700 700 c, g, t dbSNP:373352523
701 701 a, g dbSNP:141132227
703 703 a, t dbSNP:770528169
725 725 a, g dbSNP:11575907
732 732 c, t dbSNP:778456028
735 735 a, g dbSNP:772659245
742 742 a, c dbSNP:778741202
763 763 a, g dbSNP:770667884
765 765 a, g dbSNP:376049376
772 772 a, g dbSNP:772792120
775 775 a, g dbSNP:373364805
786 786 c, t dbSNP:369377287
795 795 c, t dbSNP:201921473
797 797 a, c, t dbSNP:2070121
798 798 a, c, g, t dbSNP:112802855
799 799 a, t dbSNP:552889114
805 805 a, g dbSNP:769104615
811 811 c, t dbSNP:55816512
818 818 g, t dbSNP:780341623
819 819 g, t dbSNP:2071478
826 826 c, t dbSNP:558387026
827 827 a, g dbSNP:2621330
828 828 c, t dbSNP:111971502
845 845 c, t dbSNP:113536280
851 851 a, g dbSNP:201484149
864 864 a, c, t dbSNP:778935736
866 866 c, g dbSNP:754818344
873 873 c, g dbSNP:201637948
877 877 c, t dbSNP:766332080
879 879 a, g dbSNP:143706496
881 881 a, g dbSNP:200490776
901 901 c, g dbSNP:756792582
910 910 a, g dbSNP:751218661
913 913 a, g dbSNP:763572509
914 914 c, t dbSNP:762503684
920 920 a, g dbSNP:753244529
922 922 c, t dbSNP:765694058
926 926 c, t dbSNP:759920079
928 928 a, c dbSNP:776926890
930 930 -, g dbSNP:55798049
936 936 -, g dbSNP:56040300
939 939 c, g, t dbSNP:760875082
941 941 c, t dbSNP:773458452
943 943 c, t dbSNP:772081272
948 948 a, g dbSNP:748194214
949 949 a, c dbSNP:780158534
955 955 a, g dbSNP:41258084
963 963 a, g dbSNP:745662315
964 964 a, g dbSNP:201593955
970 970 c, t dbSNP:781199237
977 977 -, c dbSNP:56150445
981 981 c, g dbSNP:200674755
985 985 a, g dbSNP:530542664
998 998 c, t dbSNP:2070120
999 999 a, g dbSNP:111466995
1027 1027 -, ct dbSNP:749857284
1032 1032 a, c, t dbSNP:548506365
1051 1051 a, g dbSNP:780226787
1076 1076 c, t dbSNP:770754630
1086 1086 c, t dbSNP:187196646
1106 1106 a, t dbSNP:559575192
1119 1119 a, g dbSNP:749306940
1136 1136 a, c dbSNP:541264930
1143 1143 a, c dbSNP:573233455
1163 1163 c, t dbSNP:777426803
1188 1188 c, t dbSNP:11244
1189 1189 a, c, g, t dbSNP:113166463
1208 1208 c, t dbSNP:545851818
1226 1226 c, t dbSNP:372376475
1228 1228 a, g dbSNP:557195146
1262 1262 a, g dbSNP:145480626
1263 1263 c, t dbSNP:535737636
1268 1268 a, g dbSNP:574841650
1272 1272 c, g dbSNP:553270743
1311 1311 c, t dbSNP:752339673
1314 1314 -, c dbSNP:35340893
1335 1335 c, t dbSNP:535257172
1339 1339 a, g dbSNP:11537545
1340 1340 a, g dbSNP:10364

Target ORF information:

RefSeq Version NM_002120
Organism Homo sapiens (human)
Definition Homo sapiens major histocompatibility complex, class II, DO beta (HLA-DOB), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002120

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGTTCTG GGTGGGTCCC CTGGGTGGTG GCTCTGCTAG TGAATCTGAC CCGACTGGAT 
TCCTCCATGA CTCAAGGCAC AGACTCTCCA GAAGATTTTG TGATTCAGGC AAAGGCTGAC
TGTTACTTCA CCAACGGGAC AGAAAAGGTG CAGTTTGTGG TCAGATTCAT CTTTAACTTG
GAGGAGTATG TACGTTTCGA CAGTGATGTG GGGATGTTTG TGGCATTGAC CAAGCTGGGG
CAGCCAGATG CTGAGCAGTG GAACAGCCGG CTGGATCTCT TGGAGAGGAG CAGACAGGCC
GTGGATGGGG TCTGTAGACA CAACTACAGG CTGGGCGCAC CCTTCACTGT GGGGAGAAAA
GTGCAACCAG AGGTGACAGT GTACCCAGAG AGGACCCCAC TCCTGCACCA GCATAATCTG
CTGCACTGCT CTGTGACAGG CTTCTATCCA GGGGATATCA AGATCAAGTG GTTCCTGAAT
GGGCAGGAGG AGAGAGCTGG GGTCATGTCC ACTGGCCCTA TCAGGAATGG AGACTGGACC
TTTCAGACTG TGGTGATGCT AGAAATGACT CCTGAACTTG GACATGTCTA CACCTGCCTT
GTCGATCACT CCAGCCTGCT GAGCCCTGTT TCTGTGGAGT GGAGAGCTCA GTCTGAATAT
TCTTGGAGAA AGATGCTGAG TGGCATTGCA GCCTTCCTAC TTGGGCTAAT CTTCCTTCTG
GTGGGAATCG TCATCCAGCT AAGGGCTCAG AAAGGATATG TGAGGACGCA GATGTCTGGT
AATGAGGTCT CAAGAGCTGT TCTGCTCCCT CAGTCATGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.