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HLA-DOB cDNA ORF clone, Homo sapiens (human)

Gene Symbol HLA-DOB
Entrez Gene ID 3112
Full Name major histocompatibility complex, class II, DO beta
Synonyms DOB
General protein information
Preferred Names
HLA class II histocompatibility antigen, DO beta chain
Names
HLA class II histocompatibility antigen, DO beta chain
MHC class II antigen DOB
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p21.3

Summary HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

600629


mRNA and Protein(s)

mRNA Protein Name
NM_002120 NP_002111 HLA class II histocompatibility antigen, DO beta chain precursor



Homo sapiens (human) HLA-DOB NP_002111.1
Pan troglodytes (chimpanzee) PATR-DOB NP_001123943.1
Macaca mulatta (Rhesus monkey) MAMU-DOB XP_001115393.1
Canis lupus familiaris (dog) DLA-DOB NP_001041592.1
Bos taurus (cattle) BOLA-DOB NP_001013618.1
Mus musculus (house mouse) H2-Ob NP_034519.2
Rattus norvegicus (Norway rat) RT1-DOb NP_001008846.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HLA-DOB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HLA-DOB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19686
NM_002120 Homo sapiens major histocompatibility complex, class II, DO beta (HLA-DOB), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$154.50-$216.30
$309.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
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30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19686
Clone ID Related Accession (Same CDS sequence) NM_002120
Accession Version NM_002120.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 822bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product HLA class II histocompatibility antigen, DO beta chain precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM152694.1, BC006097.1 and BQ010608.1. This sequence is a reference standard in the RefSeqGene project. On Nov 24, 2006 this sequence version replaced gi:18641377. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006097.1, AY645723.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGTTCTG GGTGGGTCCC CTGGGTGGTG GCTCTGCTAG TGAATCTGAC CCGACTGGAT 
TCCTCCATGA CTCAAGGCAC AGACTCTCCA GAAGATTTTG TGATTCAGGC AAAGGCTGAC
TGTTACTTCA CCAACGGGAC AGAAAAGGTG CAGTTTGTGG TCAGATTCAT CTTTAACTTG
GAGGAGTATG TACGTTTCGA CAGTGATGTG GGGATGTTTG TGGCATTGAC CAAGCTGGGG
CAGCCAGATG CTGAGCAGTG GAACAGCCGG CTGGATCTCT TGGAGAGGAG CAGACAGGCC
GTGGATGGGG TCTGTAGACA CAACTACAGG CTGGGCGCAC CCTTCACTGT GGGGAGAAAA
GTGCAACCAG AGGTGACAGT GTACCCAGAG AGGACCCCAC TCCTGCACCA GCATAATCTG
CTGCACTGCT CTGTGACAGG CTTCTATCCA GGGGATATCA AGATCAAGTG GTTCCTGAAT
GGGCAGGAGG AGAGAGCTGG GGTCATGTCC ACTGGCCCTA TCAGGAATGG AGACTGGACC
TTTCAGACTG TGGTGATGCT AGAAATGACT CCTGAACTTG GACATGTCTA CACCTGCCTT
GTCGATCACT CCAGCCTGCT GAGCCCTGTT TCTGTGGAGT GGAGAGCTCA GTCTGAATAT
TCTTGGAGAA AGATGCTGAG TGGCATTGCA GCCTTCCTAC TTGGGCTAAT CTTCCTTCTG
GTGGGAATCG TCATCCAGCT AAGGGCTCAG AAAGGATATG TGAGGACGCA GATGTCTGGT
AATGAGGTCT CAAGAGCTGT TCTGCTCCCT CAGTCATGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002111.1
CDS98..919
Misc Feature(1)176..457(+)
Misc Feature(2)212..436(+)
Misc Feature(3)458..739(+)
Misc Feature(4)464..745(+)
Misc Feature(5)740..769(+)
Misc Feature(6)770..832(+)
Exon (1)1..188
Gene:HLA-DOB
Gene Synonym:
Exon (2)189..458
Gene:HLA-DOB
Gene Synonym:
Exon (3)459..740
Gene:HLA-DOB
Gene Synonym:
Exon (4)741..851
Gene:HLA-DOB
Gene Synonym:
Exon (5)852..883
Gene:HLA-DOB
Gene Synonym:
Exon (6)884..1372
Gene:HLA-DOB
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002120
Organism Homo sapiens (human)
Definition Homo sapiens major histocompatibility complex, class II, DO beta (HLA-DOB), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002120

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGTTCTG GGTGGGTCCC CTGGGTGGTG GCTCTGCTAG TGAATCTGAC CCGACTGGAT 
TCCTCCATGA CTCAAGGCAC AGACTCTCCA GAAGATTTTG TGATTCAGGC AAAGGCTGAC
TGTTACTTCA CCAACGGGAC AGAAAAGGTG CAGTTTGTGG TCAGATTCAT CTTTAACTTG
GAGGAGTATG TACGTTTCGA CAGTGATGTG GGGATGTTTG TGGCATTGAC CAAGCTGGGG
CAGCCAGATG CTGAGCAGTG GAACAGCCGG CTGGATCTCT TGGAGAGGAG CAGACAGGCC
GTGGATGGGG TCTGTAGACA CAACTACAGG CTGGGCGCAC CCTTCACTGT GGGGAGAAAA
GTGCAACCAG AGGTGACAGT GTACCCAGAG AGGACCCCAC TCCTGCACCA GCATAATCTG
CTGCACTGCT CTGTGACAGG CTTCTATCCA GGGGATATCA AGATCAAGTG GTTCCTGAAT
GGGCAGGAGG AGAGAGCTGG GGTCATGTCC ACTGGCCCTA TCAGGAATGG AGACTGGACC
TTTCAGACTG TGGTGATGCT AGAAATGACT CCTGAACTTG GACATGTCTA CACCTGCCTT
GTCGATCACT CCAGCCTGCT GAGCCCTGTT TCTGTGGAGT GGAGAGCTCA GTCTGAATAT
TCTTGGAGAA AGATGCTGAG TGGCATTGCA GCCTTCCTAC TTGGGCTAAT CTTCCTTCTG
GTGGGAATCG TCATCCAGCT AAGGGCTCAG AAAGGATATG TGAGGACGCA GATGTCTGGT
AATGAGGTCT CAAGAGCTGT TCTGCTCCCT CAGTCATGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Ubiquitination of HLA-DO by MARCH family E3 ligases
Eur. J. Immunol. 43 (5), 1153-1161 (2013)
Jahnke M, Trowsdale J and Kelly AP.


book

A genome-wide association study identifies 2 susceptibility Loci for Crohn's disease in a Japanese population
Gastroenterology 144 (4), 781-788 (2013)
Yamazaki K, Umeno J, Takahashi A, Hirano A, Johnson TA, Kumasaka N, Morizono T, Hosono N, Kawaguchi T, Takazoe M, Yamada T, Suzuki Y, Tanaka H, Motoya S, Hosokawa M, Arimura Y, Shinomura Y, Matsui T, Matsumoto T, Iida M, Tsunoda T, Nakamura Y, Kamatani N and Kubo M.


book

HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism
Nat. Struct. Mol. Biol. 20 (1), 90-98 (2013)
Guce AI, Mortimer SE, Yoon T, Painter CA, Jiang W, Mellins ED and Stern LJ.


book

A genome-wide association study identifies three new risk loci for Kawasaki disease
Nat. Genet. 44 (5), 517-521 (2012)
Onouchi Y, Ozaki K, Burns JC, Shimizu C, Terai M, Hamada H, Honda T, Suzuki H, Suenaga T, Takeuchi T, Yoshikawa N, Suzuki Y, Yasukawa K, Ebata R, Higashi K, Saji T, Kemmotsu Y, Takatsuki S, Ouchi K, Kishi F, Yoshikawa T, Nagai T, Hamamoto K, Sato Y, Honda A, Kobayashi H, Sato J, Shibuta S, Miyawaki M, Oishi K, Yamaga H, Aoyagi N, Iwahashi S, Miyashita R, Murata Y, Sasago K, Takahashi A, Kamatani N, Kubo M, Tsunoda T, Hata A, Nakamura Y and Tanaka T.


book

New genetic associations detected in a host response study to hepatitis B vaccine
Genes Immun. 11 (3), 232-238 (2010)
Davila S, Froeling FE, Tan A, Bonnard C, Boland GJ, Snippe H, Hibberd ML and Seielstad M.


book

The major histocompatibility complex-encoded proteasome component LMP7: alternative first exons and post-translational processing
Eur. J. Immunol. 23 (4), 860-866 (1993)
Glynne R, Kerr LA, Mockridge I, Beck S, Kelly A and Trowsdale J.


book

DNA sequence analysis of 66 kb of the human MHC class II region encoding a cluster of genes for antigen processing
J. Mol. Biol. 228 (2), 433-441 (1992)
Beck S, Kelly A, Radley E, Khurshid F, Alderton RP and Trowsdale J.


book

Human class II DNA and DOB genes display low sequence variability
Immunogenetics 29 (6), 411-413 (1989)
Jonsson AK and Rask L.


book

Class II genes of the human major histocompatibility complex. The DO beta gene is a divergent member of the class II beta gene family
J. Biol. Chem. 262 (18), 8759-8766 (1987)
Servenius,B., Rask,L. and Peterson,P.A.


book

DO beta: a new beta chain gene in HLA-D with a distinct regulation of expression
EMBO J. 4 (11), 2839-2847 (1985)
Tonnelle,C., DeMars,R. and Long,E.O.


 
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