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HLA-DQA1 major histocompatibility complex, class II, DQ alpha 1 [Homo sapiens (human)]

Gene Symbol HLA-DQA1
Entrez Gene ID 3117
Full Name major histocompatibility complex, class II, DQ alpha 1
Synonyms CD, CELIAC1, DQ-A1, GSE, HLA-DQA
General protein information
Preferred Names
HLA class II histocompatibility antigen, DQ alpha 1 chain
Names
HLA class II histocompatibility antigen, DQ alpha 1 chain
HLA-DCA
DC-alpha
DC-1 alpha chain
MHC HLA-DQ alpha
MHC class II DQA1
MHC class II antigen
leucocyte antigen DQA1
MHC class II HLA-DQ-alpha-1
leukocyte antigen alpha chain
MHC class II surface glycoprotein
MHC class II HLA-D alpha glycoprotein
HLA class II histocompatibility antigen, DQ(W3) alpha chain
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p21.3

Summary HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

146880

Disorder Html: {Celiac disease, susceptibility to}, 212750 (3)





The following HLA-DQA1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HLA-DQA1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu40288 XM_006715079 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ alpha 1 (HLA-DQA1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu54837 XM_006725484 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ alpha 1 (HLA-DQA1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $199
OHu32852 XM_005274953 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ alpha 1 (HLA-DQA1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $199
OHu58237 XM_011547645 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ alpha 1 (HLA-DQA1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu32852 XM_005275108 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ alpha 1 (HLA-DQA1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $199
OHu58237 XM_011547876 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ alpha 1 (HLA-DQA1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu55023 XM_003846468 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ alpha 1 (HLA-DQA1), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu54837 XM_006725999 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ alpha 1 (HLA-DQA1), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $199
OHu55079 XM_005275542 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ alpha 1 (HLA-DQA1), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $199
OHu18636 NM_002122 Homo sapiens major histocompatibility complex, class II, DQ alpha 1 (HLA-DQA1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199

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**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu40288
Accession Version XM_006715079.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 621bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product HLA class II histocompatibility antigen, DQ alpha 1 chain isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007592.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578811643. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGATCCTAA ACAAAGCTCT GCTGCTGGGG GCCCTCGCTC TGACCACCGT GATGAGCCCC 
TGTGGAGGTG AAGACATTGT GGCTGACCAC GTTGCCTCTT GTGGTGTAAA CTTGTACCAG
TTTTACGGTC CCTCTGGCCA GTACACCCAT GAATTTGATG GAGATGAGCA GTTCTACGTG
GACCTGGAGA GGAAGGAGAC TGCCTGGCGG TGGCCTGAGT TCAGCAAATT TGGAGGTTTT
GACCCGCAGG GTGCACTGAG AAACATGGCT GTGGCAAAAC ACAACTTGAA CATCATGATT
AAACGCTACA ACTCTACCGC TGCTACCAAT GAGGTTCCTG AGGTCACAGT GTTTTCCAAG
TCTCCCGTGA CACTGGGTCA GCCCAACACC CTCATTTGTC TTGTGGACAA CATCTTTCCT
CCTGTGGTCA ACATCACATG GCTGAGCAAT GGGCAGTCAG TCACAGAAGG TGTTTCTGAG
ACCAGCTTCC TCTCCAAGAG TGATCATTCC TTCTTCAAGA TCAGTTACCT CACCTTCCTC
CCTTCTGCTG ATGAGATTTA TGACTGCAAG GTGGAGCACT GGGGCCTGGA CCAGCCTCTT
CTGAAACACT GGGTTGAATG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006715142.1
CDS64..684
Misc Feature(1)148..393(+)
Misc Feature(2)397..678(+)
Misc Feature(3)409..591(+)
Misc Feature(4)439..642(+)
Misc Feature(5)481..591(+)
Translation
Position Chain Variation Link
1 1 c, t dbSNP:9272424
5 5 a, g dbSNP:567258915
6 6 c, t dbSNP:9272425
13 13 a, t dbSNP:751610786
14 14 a, c, g dbSNP:761951737
16 16 a, t dbSNP:750601509
17 17 a, c, g, t dbSNP:9272426
18 18 c, t dbSNP:755486767
20 20 c, g, t dbSNP:779603527
23 23 a, c, t dbSNP:771761512
24 24 a, g dbSNP:746602382
25 25 a, g dbSNP:3207966
27 27 a, t dbSNP:776466842
30 30 g, t dbSNP:759291994
32 32 a, g dbSNP:200004305
35 35 a, c, g dbSNP:3187964
36 36 a, c dbSNP:767518956
38 38 a, g dbSNP:773197867
44 44 a, g dbSNP:1047985
47 47 a, g dbSNP:766556976
48 48 c, t dbSNP:754198500
50 50 g, t dbSNP:755460098
60 60 a, g dbSNP:765825504
62 62 a, g dbSNP:28383345
66 66 a, g dbSNP:758975009
76 76 a, g dbSNP:777374378
80 80 c, t dbSNP:746521319
84 84 a, g dbSNP:756905853
85 85 a, c dbSNP:1047989
93 93 g, t dbSNP:745666028
94 94 a, g, t dbSNP:1047992
96 96 a, c, t dbSNP:749185114
99 99 c, g, t dbSNP:1047993
102 102 a, c, g, t dbSNP:1130034
103 103 c, g dbSNP:776775819
110 110 c, t dbSNP:369177390
111 111 c, t dbSNP:41551712
112 112 a, g dbSNP:12722039
113 113 c, t dbSNP:758923598
116 116 c, t dbSNP:11545686
119 119 c, g dbSNP:751079577
120 120 c, t dbSNP:756778447
123 123 c, t dbSNP:1129735
124 124 c, t dbSNP:745457579
133 133 a, g dbSNP:41545012
137 137 a, g dbSNP:12722042
141 141 a, t dbSNP:749132342
145 145 a, g dbSNP:373782144
153 153 c, t dbSNP:1129737
156 156 c, t dbSNP:1129738
158 158 c, t dbSNP:763996857
163 163 -, tg dbSNP:776891401
164 164 a, g dbSNP:1129740
165 165 -, aac dbSNP:746358168
165 165 c, t dbSNP:3205916
169 169 a, g dbSNP:780232299
171 171 a, g dbSNP:1129742
175 175 a, t dbSNP:749434805
177 177 a, c, g dbSNP:1129744
185 185 c, t dbSNP:1071630
189 189 c, t dbSNP:1129753
190 190 a, g dbSNP:772362984
194 194 c, t dbSNP:41549715
201 201 c, g dbSNP:1129759
206 206 a, t dbSNP:12722051
209 209 c, g dbSNP:1048023
221 221 a, g dbSNP:775981593
223 223 g, t dbSNP:759125166
224 224 a, g, t dbSNP:112029808
226 226 g, t dbSNP:775152680
227 227 a, c, g dbSNP:762518779
228 228 c, t dbSNP:1048027
232 232 c, g dbSNP:10093
239 239 a, t dbSNP:767607207
240 240 c, t dbSNP:1129765
251 251 a, g dbSNP:1142323
254 254 a, g dbSNP:36219699
255 255 g, t dbSNP:752894641
258 258 g, t dbSNP:758646527
262 262 a, g dbSNP:41543221
266 266 c, t dbSNP:1142324
271 271 a, c, t dbSNP:1142326
272 272 a, g dbSNP:3207983
273 273 g, t dbSNP:3207984
275 275 g, t dbSNP:1142328
280 280 -, ga dbSNP:760671632
280 280 c, g dbSNP:12722061
281 281 a, t dbSNP:3208105
282 282 g, t dbSNP:9272697
282 282 g, t dbSNP:546754435
283 283 -, tc dbSNP:764189141
283 283 a, c, t dbSNP:9272698
283 283 c, t dbSNP:568289532
284 284 -, cca dbSNP:763406304
286 286 -, ag dbSNP:767108045
286 286 a, c, t dbSNP:3207985
287 287 -, a dbSNP:200303565
287 287 a, g dbSNP:28383449
287 287 -, g dbSNP:772825532
288 288 a, c dbSNP:9272699
288 288 a, c dbSNP:568465013
289 289 -, gac dbSNP:765866325
289 289 a, c, g, t dbSNP:1048052
290 290 a, g dbSNP:12722069
292 292 c, t dbSNP:3188043
295 295 -, gg dbSNP:753442670
295 295 a, g, t dbSNP:4193
296 296 g, t dbSNP:758597345
298 298 -, ggt dbSNP:534654325
298 298 a, g dbSNP:12722072
299 299 a, g dbSNP:751915559
299 299 -, g dbSNP:41556812
300 300 a, t dbSNP:12722074
304 304 g, t dbSNP:781416959
308 308 a, c, g, t dbSNP:41541412
312 312 a, g dbSNP:9272702
313 313 g, t dbSNP:1142331
314 314 g, t dbSNP:1142332
319 319 a, c dbSNP:761573168
320 320 c, t dbSNP:771830448
322 322 a, t dbSNP:772909319
323 323 c, g dbSNP:1142333
324 324 a, g dbSNP:766272167
330 330 a, c, g dbSNP:1142334
332 332 -, tg dbSNP:765228558
333 333 c, g, t dbSNP:1048491
334 334 -, tcc dbSNP:752397622
334 334 a, g dbSNP:764286168
336 336 -, gg dbSNP:758294111
336 336 c, g dbSNP:3188501
337 337 a, c, g, t dbSNP:1142335
338 338 -, ct dbSNP:777760029
338 338 c, t dbSNP:9272709
338 338 c, t dbSNP:527759771
340 340 a, g dbSNP:756616948
343 343 c, t dbSNP:780496366
345 345 a, g dbSNP:1048087
347 347 a, c dbSNP:754481952
349 349 c, g, t dbSNP:778568255
352 352 a, g dbSNP:771641715
355 355 -, g, gt, gtc dbSNP:9282026
356 356 g, t dbSNP:9279910
357 357 -, ca dbSNP:745963770
357 357 c, t dbSNP:36219014
358 358 -, a, gtc dbSNP:371894400
358 358 -, a dbSNP:199556640
358 358 a, c, g dbSNP:1064944
367 367 c, t dbSNP:41542116
368 368 g, t dbSNP:759480677
370 370 g, t dbSNP:764120909
371 371 a, c, g dbSNP:1129808
375 375 c, g dbSNP:767673676
381 381 c, t dbSNP:1142338
382 382 a, g dbSNP:142591955
385 385 g, t dbSNP:766867329
392 392 a, c dbSNP:201351192
401 401 c, g dbSNP:760756426
420 420 a, c dbSNP:771325735
425 425 c, t dbSNP:1048122
429 429 c, t dbSNP:1048124
430 430 a, g dbSNP:368821483
432 432 g, t dbSNP:765825432
435 435 a, g dbSNP:1048134
436 436 c, g dbSNP:41555012
452 452 c, t dbSNP:707952
456 456 c, t dbSNP:751132570
459 459 c, t dbSNP:707951
463 463 c, t dbSNP:780889985
465 465 g, t dbSNP:750209003
468 468 a, g dbSNP:755849378
475 475 a, t dbSNP:779906935
501 501 a, c, g dbSNP:1048173
503 503 a, g dbSNP:777689650
507 507 a, g dbSNP:747148636
515 515 g, t dbSNP:771270725
519 519 cg, gt dbSNP:386699556
519 519 c, g, t dbSNP:707950
520 520 g, t dbSNP:41547417
523 523 a, c, g dbSNP:369452859
530 530 a, g dbSNP:763347786
543 543 c, g dbSNP:764703448
545 545 a, c, t dbSNP:41545514
547 547 a, c dbSNP:41552014
548 548 g, t dbSNP:41544114
566 566 a, t dbSNP:755797601
575 575 c, t dbSNP:766043000
580 580 a, g dbSNP:753712484
584 584 a, t dbSNP:754837184
586 586 a, g dbSNP:778972437
589 589 c, t dbSNP:41550317
595 595 a, g dbSNP:757353300
598 598 c, t dbSNP:707949
601 601 c, t dbSNP:746044970
610 610 g, t dbSNP:41561312
611 611 a, c dbSNP:7990
613 613 a, g dbSNP:749728161
615 615 g, t dbSNP:707963
620 620 g, t dbSNP:707962
634 634 g, t dbSNP:762316160
637 637 c, g dbSNP:766977165
639 639 a, g dbSNP:772733847
642 642 c, t dbSNP:760295892
643 643 g, t dbSNP:766092893
648 648 a, c dbSNP:2308889
652 652 a, g dbSNP:774878538
653 653 a, c dbSNP:754856407
654 654 a, c, t dbSNP:2308890
655 655 a, c, g dbSNP:2308891
672 672 a, c dbSNP:745985669
675 675 a, g dbSNP:756288178
687 687 c, g dbSNP:17613507
698 698 a, g dbSNP:17613514
711 711 c, t dbSNP:17613521
716 716 a, g dbSNP:17843711
730 730 c, t dbSNP:17843712
731 731 a, c dbSNP:17843713

Target ORF information:

RefSeq Version XM_006715079
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ alpha 1 (HLA-DQA1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006715079

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGATCCTAA ACAAAGCTCT GCTGCTGGGG GCCCTCGCTC TGACCACCGT GATGAGCCCC 
TGTGGAGGTG AAGACATTGT GGCTGACCAC GTTGCCTCTT GTGGTGTAAA CTTGTACCAG
TTTTACGGTC CCTCTGGCCA GTACACCCAT GAATTTGATG GAGATGAGCA GTTCTACGTG
GACCTGGAGA GGAAGGAGAC TGCCTGGCGG TGGCCTGAGT TCAGCAAATT TGGAGGTTTT
GACCCGCAGG GTGCACTGAG AAACATGGCT GTGGCAAAAC ACAACTTGAA CATCATGATT
AAACGCTACA ACTCTACCGC TGCTACCAAT GAGGTTCCTG AGGTCACAGT GTTTTCCAAG
TCTCCCGTGA CACTGGGTCA GCCCAACACC CTCATTTGTC TTGTGGACAA CATCTTTCCT
CCTGTGGTCA ACATCACATG GCTGAGCAAT GGGCAGTCAG TCACAGAAGG TGTTTCTGAG
ACCAGCTTCC TCTCCAAGAG TGATCATTCC TTCTTCAAGA TCAGTTACCT CACCTTCCTC
CCTTCTGCTG ATGAGATTTA TGACTGCAAG GTGGAGCACT GGGGCCTGGA CCAGCCTCTT
CTGAAACACT GGGTTGAATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu54837
Accession Version XM_006725484.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 765bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product HLA class II histocompatibility antigen, DQ alpha 1 chain isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_113891.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578840832. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGATCCTAA ACAAAGCTCT GATGCTGGGG GCCCTTGCCC TGACCACCGT GATGAGCCCC 
TGTGGAGGTG AAGACATTGT GGCTGACCAC GTCGCCTCTT ATGGTGTAAA CTTGTACCAG
TCTTACGGTC CCTCTGGCCA GTACACCCAT GAATTTGATG GAGATGAGCA GTTCTACGTG
GACCTGGGGA GGAAGGAGAC TGTCTGGTGT TTGCCTGTTC TCAGACAATT TAGATTTGAC
CCGCAATTTG CACTGACAAA CATCGCTGTC CTAAAACATA ACTTGAACAG TCTGATTAAA
CGCTCCAACT CTACCGCTGC TACCAATGAG GTTCCTGAGG TCACAGTGTT TTCCAAGTCT
CCCGTGACAC TGGGTCAGCC CAACATCCTC ATCTGTCTTG TGGACAACAT CTTTCCTCCT
GTGGTCAACA TCACATGGCT GAGCAATGGG CACTCAGTCA CAGAAGGTGT TTCTGAGACC
AGCTTCCTCT CCAAGAGTGA TCATTCCTTC TTCAAGATCA GTTACCTCAC CCTCCTCCCT
TCTGCTGAGG AGAGTTATGA CTGCAAGGTG GAGCACTGGG GCCTGGACAA GCCTCTTCTG
AAACACTGGG AGCCTGAGAT TCCAGCCCCT ATGTCAGAGC TCACAGAGAC TGTGGTCTGC
GCCCTGGGAT TGTCTGTGGG CCTCGTGGGC ATTGTGGTGG GCACTGTCTT CATCATCCGA
GGCCTGCGTT CAGTTGGTGC TTCCAGACAC CAAGGGCCCT TGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006725547.1
CDS65..829
Misc Feature(1)149..391(+)
Misc Feature(2)395..676(+)
Misc Feature(3)407..589(+)
Misc Feature(4)479..589(+)
Translation
Position Chain Variation Link
2 2 c, t dbSNP:9272424
6 6 a, g dbSNP:567258915
7 7 c, t dbSNP:9272425
14 14 a, t dbSNP:751610786
15 15 a, c, g dbSNP:761951737
17 17 a, t dbSNP:750601509
18 18 a, c, g, t dbSNP:9272426
19 19 c, t dbSNP:755486767
21 21 c, g, t dbSNP:779603527
24 24 a, c, t dbSNP:771761512
25 25 a, g dbSNP:746602382
26 26 a, g dbSNP:3207966
28 28 a, t dbSNP:776466842
31 31 g, t dbSNP:759291994
33 33 a, g dbSNP:200004305
36 36 a, c, g dbSNP:3187964
37 37 a, c dbSNP:767518956
39 39 a, g dbSNP:773197867
45 45 a, g dbSNP:1047985
48 48 a, g dbSNP:766556976
49 49 c, t dbSNP:754198500
51 51 g, t dbSNP:755460098
61 61 a, g dbSNP:765825504
63 63 a, g dbSNP:28383345
67 67 a, g dbSNP:758975009
77 77 a, g dbSNP:777374378
81 81 c, t dbSNP:746521319
85 85 a, g dbSNP:756905853
86 86 a, c dbSNP:1047989
94 94 g, t dbSNP:745666028
95 95 a, g, t dbSNP:1047992
97 97 a, c, t dbSNP:749185114
100 100 c, g, t dbSNP:1047993
103 103 a, c, g, t dbSNP:1130034
104 104 c, g dbSNP:776775819
111 111 c, t dbSNP:369177390
112 112 c, t dbSNP:41551712
113 113 a, g dbSNP:12722039
114 114 c, t dbSNP:758923598
117 117 c, t dbSNP:11545686
120 120 c, g dbSNP:751079577
121 121 c, t dbSNP:756778447
124 124 c, t dbSNP:1129735
125 125 c, t dbSNP:745457579
134 134 a, g dbSNP:41545012
138 138 a, g dbSNP:12722042
142 142 a, t dbSNP:749132342
146 146 a, g dbSNP:373782144
154 154 c, t dbSNP:1129737
157 157 c, t dbSNP:1129738
159 159 c, t dbSNP:763996857
164 164 -, tg dbSNP:776891401
165 165 a, g dbSNP:1129740
166 166 -, aac dbSNP:746358168
166 166 c, t dbSNP:3205916
170 170 a, g dbSNP:780232299
172 172 a, g dbSNP:1129742
176 176 a, t dbSNP:749434805
178 178 a, c, g dbSNP:1129744
186 186 c, t dbSNP:1071630
190 190 c, t dbSNP:1129753
191 191 a, g dbSNP:772362984
195 195 c, t dbSNP:41549715
202 202 c, g dbSNP:1129759
207 207 a, t dbSNP:12722051
210 210 c, g dbSNP:1048023
222 222 a, g dbSNP:775981593
224 224 g, t dbSNP:759125166
225 225 a, g, t dbSNP:112029808
227 227 g, t dbSNP:775152680
228 228 a, c, g dbSNP:762518779
229 229 c, t dbSNP:1048027
233 233 c, g dbSNP:10093
240 240 a, t dbSNP:767607207
241 241 c, t dbSNP:1129765
252 252 a, g dbSNP:1142323
255 255 a, g dbSNP:36219699
256 256 g, t dbSNP:752894641
259 259 g, t dbSNP:758646527
263 263 a, g dbSNP:41543221
267 267 c, t dbSNP:1142324
272 272 a, c, t dbSNP:1142326
273 273 a, g dbSNP:3207983
274 274 g, t dbSNP:3207984
276 276 g, t dbSNP:1142328
281 281 -, ga dbSNP:760671632
281 281 c, g dbSNP:12722061
282 282 a, t dbSNP:3208105
283 283 g, t dbSNP:9272697
283 283 g, t dbSNP:546754435
284 284 -, tc dbSNP:764189141
284 284 a, c, t dbSNP:9272698
284 284 c, t dbSNP:568289532
285 285 -, cca dbSNP:763406304
287 287 -, ag dbSNP:767108045
287 287 a, c, t dbSNP:3207985
288 288 -, a dbSNP:200303565
288 288 a, g dbSNP:28383449
288 288 -, g dbSNP:772825532
289 289 a, c dbSNP:9272699
289 289 a, c dbSNP:568465013
290 290 -, gac dbSNP:765866325
290 290 a, c, g, t dbSNP:1048052
291 291 a, g dbSNP:12722069
293 293 c, t dbSNP:3188043
296 296 -, gg dbSNP:753442670
296 296 a, g, t dbSNP:4193
297 297 g, t dbSNP:758597345
302 302 g, t dbSNP:781416959
306 306 a, c, g, t dbSNP:41541412
310 310 a, g dbSNP:9272702
311 311 g, t dbSNP:1142331
312 312 g, t dbSNP:1142332
317 317 a, c dbSNP:761573168
318 318 c, t dbSNP:771830448
320 320 a, t dbSNP:772909319
321 321 c, g dbSNP:1142333
322 322 a, g dbSNP:766272167
328 328 a, c, g dbSNP:1142334
330 330 -, tg dbSNP:765228558
331 331 c, g, t dbSNP:1048491
332 332 -, tcc dbSNP:752397622
332 332 a, g dbSNP:764286168
334 334 -, gg dbSNP:758294111
334 334 c, g dbSNP:3188501
335 335 a, c, g, t dbSNP:1142335
336 336 -, ct dbSNP:777760029
336 336 c, t dbSNP:9272709
336 336 c, t dbSNP:527759771
338 338 a, g dbSNP:756616948
341 341 c, t dbSNP:780496366
343 343 a, g dbSNP:1048087
345 345 a, c dbSNP:754481952
347 347 c, g, t dbSNP:778568255
350 350 a, g dbSNP:771641715
353 353 -, g, gt, gtc dbSNP:9282026
354 354 g, t dbSNP:9279910
355 355 -, ca dbSNP:745963770
355 355 c, t dbSNP:36219014
356 356 -, a, gtc dbSNP:371894400
356 356 -, a dbSNP:199556640
356 356 a, c, g dbSNP:1064944
365 365 c, t dbSNP:41542116
366 366 g, t dbSNP:759480677
368 368 g, t dbSNP:764120909
369 369 a, c, g dbSNP:1129808
373 373 c, g dbSNP:767673676
379 379 c, t dbSNP:1142338
380 380 a, g dbSNP:142591955
383 383 g, t dbSNP:766867329
390 390 a, c dbSNP:201351192
399 399 c, g dbSNP:760756426
418 418 a, c dbSNP:771325735
423 423 c, t dbSNP:1048122
427 427 c, t dbSNP:1048124
428 428 a, g dbSNP:368821483
430 430 g, t dbSNP:765825432
433 433 a, g dbSNP:1048134
434 434 c, g dbSNP:41555012
450 450 c, t dbSNP:707952
454 454 c, t dbSNP:751132570
457 457 c, t dbSNP:707951
461 461 c, t dbSNP:780889985
463 463 g, t dbSNP:750209003
466 466 a, g dbSNP:755849378
473 473 a, t dbSNP:779906935
499 499 a, c, g dbSNP:1048173
501 501 a, g dbSNP:777689650
505 505 a, g dbSNP:747148636
513 513 g, t dbSNP:771270725
517 517 cg, gt dbSNP:386699556
517 517 c, g, t dbSNP:707950
518 518 g, t dbSNP:41547417
521 521 a, c, g dbSNP:369452859
528 528 a, g dbSNP:763347786
541 541 c, g dbSNP:764703448
543 543 a, c, t dbSNP:41545514
545 545 a, c dbSNP:41552014
546 546 g, t dbSNP:41544114
564 564 a, t dbSNP:755797601
573 573 c, t dbSNP:766043000
578 578 a, g dbSNP:753712484
582 582 a, t dbSNP:754837184
584 584 a, g dbSNP:778972437
587 587 c, t dbSNP:41550317
593 593 a, g dbSNP:757353300
596 596 c, t dbSNP:707949
599 599 c, t dbSNP:746044970
608 608 g, t dbSNP:41561312
609 609 a, c dbSNP:7990
611 611 a, g dbSNP:749728161
613 613 g, t dbSNP:707963
618 618 g, t dbSNP:707962
632 632 g, t dbSNP:762316160
635 635 c, g dbSNP:766977165
637 637 a, g dbSNP:772733847
640 640 c, t dbSNP:760295892
641 641 g, t dbSNP:766092893
646 646 a, c dbSNP:2308889
650 650 a, g dbSNP:774878538
651 651 a, c dbSNP:754856407
652 652 a, c, t dbSNP:2308890
653 653 a, c, g dbSNP:2308891
670 670 a, c dbSNP:745985669
673 673 a, g dbSNP:756288178
677 677 a, c dbSNP:761792620
682 682 a, g dbSNP:767398076
689 689 aca, gcc dbSNP:386699558
689 689 a, g dbSNP:9272785
690 690 c, t dbSNP:368763112
691 691 a, c dbSNP:3188543
696 696 c, t dbSNP:752846382
711 711 a, g dbSNP:144882737
720 720 g, t dbSNP:764392438
724 724 c, t dbSNP:1048372
725 725 a, c, g dbSNP:35087390
730 730 c, g dbSNP:746400068
732 732 a, g dbSNP:755621273
733 733 a, g dbSNP:1048381
744 744 a, g dbSNP:748903287
749 749 a, g dbSNP:9260
752 752 g, t dbSNP:41545416
759 759 c, t dbSNP:747964407
764 764 a, g dbSNP:772030388
765 765 a, g dbSNP:144742185
766 766 c, g dbSNP:1048414
769 769 c, t dbSNP:1048419
775 775 c, g dbSNP:1048430
781 781 a, c dbSNP:763137732
783 783 a, g dbSNP:1048439
783 783 a, g dbSNP:9272793
790 790 a, g dbSNP:751948582
791 791 c, t dbSNP:757506993
792 792 a, g dbSNP:767929297
797 797 a, g dbSNP:750856952
800 800 a, g dbSNP:756714305
817 817 a, g dbSNP:13379
820 820 a, g dbSNP:748852050
822 822 c, t dbSNP:754676254
823 823 a, c, g dbSNP:1130116
826 826 a, g dbSNP:771694885
833 833 c, t dbSNP:777651266
834 834 c, t dbSNP:746898931
840 840 -, g dbSNP:745533419
841 841 a, g dbSNP:3188642
844 844 -, ggg dbSNP:769393523
844 844 a, g, t dbSNP:1130117
845 845 a, g dbSNP:28400568
854 854 g, t dbSNP:192684527
861 861 a, c dbSNP:9272940
863 863 c, t dbSNP:9272941
864 864 a, g dbSNP:3208169
872 872 g, t dbSNP:9272943
874 874 a, t dbSNP:36216730
878 878 g, t dbSNP:707946
879 879 g, t dbSNP:28538060
880 880 a, g dbSNP:540435913
881 881 c, g, t dbSNP:707945
887 887 c, t dbSNP:9272948
888 888 a, t dbSNP:707944
897 897 g, t dbSNP:9272950
901 901 a, g dbSNP:183747744
902 902 c, t dbSNP:62404112
905 905 a, c dbSNP:9272951
907 907 c, t dbSNP:9272952
908 908 a, c, g dbSNP:9272953
915 915 a, c dbSNP:9272954
925 925 a, t dbSNP:9272955
927 927 g, t dbSNP:9272956
932 932 a, g dbSNP:9272957
950 950 a, c dbSNP:9272958
956 956 c, g dbSNP:1064991
961 961 a, g dbSNP:1064993
975 975 c, t dbSNP:1064994
978 978 g, t dbSNP:140118665
992 992 c, t dbSNP:36216571
993 993 a, g dbSNP:1061172
1006 1006 c, g, t dbSNP:9272962
1011 1011 c, t dbSNP:1065043
1020 1020 c, t dbSNP:1065044
1032 1032 c, g, t dbSNP:1065045
1037 1037 a, g dbSNP:1065047
1042 1042 c, t dbSNP:1065048
1052 1052 a, c dbSNP:1065049
1057 1057 a, g dbSNP:552922821
1066 1066 a, g dbSNP:9272969
1076 1076 a, t dbSNP:45512899
1078 1078 -, cg dbSNP:373235196
1078 1078 c, t dbSNP:9272970
1079 1079 c, g dbSNP:9272971
1086 1086 c, t dbSNP:9272972
1089 1089 g, t dbSNP:9272973
1092 1092 c, t dbSNP:9272974
1098 1098 a, t dbSNP:9272975
1118 1118 a, g dbSNP:9272976
1121 1121 c, t dbSNP:9272977
1122 1122 a, c dbSNP:9272978
1128 1128 -, g dbSNP:370389629
1128 1128 a, g dbSNP:9272979
1134 1134 c, t dbSNP:9272980
1139 1139 c, t dbSNP:554680493

Target ORF information:

RefSeq Version XM_006725484
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ alpha 1 (HLA-DQA1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006725484

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGATCCTAA ACAAAGCTCT GATGCTGGGG GCCCTTGCCC TGACCACCGT GATGAGCCCC 
TGTGGAGGTG AAGACATTGT GGCTGACCAC GTCGCCTCTT ATGGTGTAAA CTTGTACCAG
TCTTACGGTC CCTCTGGCCA GTACACCCAT GAATTTGATG GAGATGAGCA GTTCTACGTG
GACCTGGGGA GGAAGGAGAC TGTCTGGTGT TTGCCTGTTC TCAGACAATT TAGATTTGAC
CCGCAATTTG CACTGACAAA CATCGCTGTC CTAAAACATA ACTTGAACAG TCTGATTAAA
CGCTCCAACT CTACCGCTGC TACCAATGAG GTTCCTGAGG TCACAGTGTT TTCCAAGTCT
CCCGTGACAC TGGGTCAGCC CAACATCCTC ATCTGTCTTG TGGACAACAT CTTTCCTCCT
GTGGTCAACA TCACATGGCT GAGCAATGGG CACTCAGTCA CAGAAGGTGT TTCTGAGACC
AGCTTCCTCT CCAAGAGTGA TCATTCCTTC TTCAAGATCA GTTACCTCAC CCTCCTCCCT
TCTGCTGAGG AGAGTTATGA CTGCAAGGTG GAGCACTGGG GCCTGGACAA GCCTCTTCTG
AAACACTGGG AGCCTGAGAT TCCAGCCCCT ATGTCAGAGC TCACAGAGAC TGTGGTCTGC
GCCCTGGGAT TGTCTGTGGG CCTCGTGGGC ATTGTGGTGG GCACTGTCTT CATCATCCGA
GGCCTGCGTT CAGTTGGTGC TTCCAGACAC CAAGGGCCCT TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu32852
Accession Version XM_005274953.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 765bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product HLA class II histocompatibility antigen, DQ alpha 1 chain isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167245.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578841359. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGATCCTAA ACAAAGCTCT GATGCTGGGG GCCCTCGCCC TGACCACCGT GATGAGCCCT 
TGTGGAGGTG AAGACATTGT GGCTGACCAC GTTGCCTCTT ACGGTGTAAA CTTGTACCAG
TCTTACGGTC CCTCTGGCCA GTTCACCCAT GAATTTGATG GAGACGAGGA GTTCTATGTG
GACCTGGAGA GGAAGGAGAC TGTCTGGAAG TTGCCTCTGT TCCACAGACT TAGATTTGAC
CCGCAATTTG CACTGACAAA CATCGCTGTG CTAAAACATA ACTTGAACAT CCTGATTAAA
CGCTCCAACT CTACCGCTGC TACCAATGAG GTTCCTGAGG TCACAGTGTT TTCCAAGTCT
CCCGTGACAC TGGGTCAGCC CAACACCCTC ATCTGTCTTG TGGACAACAT CTTTCCTCCT
GTGGTCAACA TCACCTGGCT GAGCAATGGG CACTCAGTCA CAGAAGGTGT TTCTGAGACC
AGCTTCCTCT CCAAGAGTGA TCATTCCTTC TTCAAGATCA GTTACCTCAC CTTCCTCCCT
TCTGCTGATG AGATTTATGA CTGCAAGGTG GAGCACTGGG GCCTGGATGA GCCTCTTCTG
AAACACTGGG AGCCTGAGAT TCCAGCACCT ATGTCAGAGC TCACAGAGAC TGTGGTCTGT
GCCCTGGGGT TGTCTGTGGG CCTCGTGGGC ATTGTGGTGG GGACCGTCTT GATCATCCGA
GGCCTGCGTT CAGTTGGTGC TTCCAGACAC CAAGGGCCCT TGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005275010.1
CDS59..823
Misc Feature(1)143..385(+)
Misc Feature(2)389..670(+)
Misc Feature(3)401..583(+)
Misc Feature(4)473..583(+)
Translation
Position Chain Variation Link
1 1 c, t dbSNP:9272425
8 8 a, t dbSNP:751610786
9 9 a, c, g dbSNP:761951737
11 11 a, t dbSNP:750601509
12 12 a, c, g, t dbSNP:9272426
13 13 c, t dbSNP:755486767
15 15 c, g, t dbSNP:779603527
18 18 a, c, t dbSNP:771761512
19 19 a, g dbSNP:746602382
20 20 a, g dbSNP:3207966
22 22 a, t dbSNP:776466842
25 25 g, t dbSNP:759291994
27 27 a, g dbSNP:200004305
30 30 a, c, g dbSNP:3187964
31 31 a, c dbSNP:767518956
33 33 a, g dbSNP:773197867
39 39 a, g dbSNP:1047985
42 42 a, g dbSNP:766556976
43 43 c, t dbSNP:754198500
45 45 g, t dbSNP:755460098
55 55 a, g dbSNP:765825504
57 57 a, g dbSNP:28383345
61 61 a, g dbSNP:758975009
71 71 a, g dbSNP:777374378
75 75 c, t dbSNP:746521319
79 79 a, g dbSNP:756905853
80 80 a, c dbSNP:1047989
88 88 g, t dbSNP:745666028
89 89 a, g, t dbSNP:1047992
91 91 a, c, t dbSNP:749185114
94 94 c, g, t dbSNP:1047993
97 97 a, c, g, t dbSNP:1130034
98 98 c, g dbSNP:776775819
105 105 c, t dbSNP:369177390
106 106 c, t dbSNP:41551712
107 107 a, g dbSNP:12722039
108 108 c, t dbSNP:758923598
111 111 c, t dbSNP:11545686
114 114 c, g dbSNP:751079577
115 115 c, t dbSNP:756778447
118 118 c, t dbSNP:1129735
119 119 c, t dbSNP:745457579
128 128 a, g dbSNP:41545012
132 132 a, g dbSNP:12722042
136 136 a, t dbSNP:749132342
140 140 a, g dbSNP:373782144
148 148 c, t dbSNP:1129737
151 151 c, t dbSNP:1129738
153 153 c, t dbSNP:763996857
158 158 -, tg dbSNP:776891401
159 159 a, g dbSNP:1129740
160 160 -, aac dbSNP:746358168
160 160 c, t dbSNP:3205916
164 164 a, g dbSNP:780232299
166 166 a, g dbSNP:1129742
170 170 a, t dbSNP:749434805
172 172 a, c, g dbSNP:1129744
180 180 c, t dbSNP:1071630
184 184 c, t dbSNP:1129753
185 185 a, g dbSNP:772362984
189 189 c, t dbSNP:41549715
196 196 c, g dbSNP:1129759
201 201 a, t dbSNP:12722051
204 204 c, g dbSNP:1048023
216 216 a, g dbSNP:775981593
218 218 g, t dbSNP:759125166
219 219 a, g, t dbSNP:112029808
221 221 g, t dbSNP:775152680
222 222 a, c, g dbSNP:762518779
223 223 c, t dbSNP:1048027
227 227 c, g dbSNP:10093
234 234 a, t dbSNP:767607207
235 235 c, t dbSNP:1129765
246 246 a, g dbSNP:1142323
249 249 a, g dbSNP:36219699
250 250 g, t dbSNP:752894641
253 253 g, t dbSNP:758646527
257 257 a, g dbSNP:41543221
261 261 c, t dbSNP:1142324
266 266 a, c, t dbSNP:1142326
267 267 a, g dbSNP:3207983
268 268 g, t dbSNP:3207984
270 270 g, t dbSNP:1142328
275 275 -, ga dbSNP:760671632
275 275 c, g dbSNP:12722061
276 276 a, t dbSNP:3208105
277 277 g, t dbSNP:9272697
277 277 g, t dbSNP:546754435
278 278 -, tc dbSNP:764189141
278 278 a, c, t dbSNP:9272698
278 278 c, t dbSNP:568289532
279 279 -, cca dbSNP:763406304
281 281 -, ag dbSNP:767108045
281 281 a, c, t dbSNP:3207985
282 282 -, a dbSNP:200303565
282 282 a, g dbSNP:28383449
282 282 -, g dbSNP:772825532
283 283 a, c dbSNP:9272699
283 283 a, c dbSNP:568465013
284 284 -, gac dbSNP:765866325
284 284 a, c, g, t dbSNP:1048052
285 285 a, g dbSNP:12722069
287 287 c, t dbSNP:3188043
290 290 -, gg dbSNP:753442670
290 290 a, g, t dbSNP:4193
291 291 g, t dbSNP:758597345
296 296 g, t dbSNP:781416959
300 300 a, c, g, t dbSNP:41541412
304 304 a, g dbSNP:9272702
305 305 g, t dbSNP:1142331
306 306 g, t dbSNP:1142332
311 311 a, c dbSNP:761573168
312 312 c, t dbSNP:771830448
314 314 a, t dbSNP:772909319
315 315 c, g dbSNP:1142333
316 316 a, g dbSNP:766272167
322 322 a, c, g dbSNP:1142334
324 324 -, tg dbSNP:765228558
325 325 c, g, t dbSNP:1048491
326 326 -, tcc dbSNP:752397622
326 326 a, g dbSNP:764286168
328 328 -, gg dbSNP:758294111
328 328 c, g dbSNP:3188501
329 329 a, c, g, t dbSNP:1142335
330 330 -, ct dbSNP:777760029
330 330 c, t dbSNP:9272709
330 330 c, t dbSNP:527759771
332 332 a, g dbSNP:756616948
335 335 c, t dbSNP:780496366
337 337 a, g dbSNP:1048087
339 339 a, c dbSNP:754481952
341 341 c, g, t dbSNP:778568255
344 344 a, g dbSNP:771641715
347 347 -, g, gt, gtc dbSNP:9282026
348 348 g, t dbSNP:9279910
349 349 -, ca dbSNP:745963770
349 349 c, t dbSNP:36219014
350 350 -, a, gtc dbSNP:371894400
350 350 -, a dbSNP:199556640
350 350 a, c, g dbSNP:1064944
359 359 c, t dbSNP:41542116
360 360 g, t dbSNP:759480677
362 362 g, t dbSNP:764120909
363 363 a, c, g dbSNP:1129808
367 367 c, g dbSNP:767673676
373 373 c, t dbSNP:1142338
374 374 a, g dbSNP:142591955
377 377 g, t dbSNP:766867329
384 384 a, c dbSNP:201351192
393 393 c, g dbSNP:760756426
412 412 a, c dbSNP:771325735
417 417 c, t dbSNP:1048122
421 421 c, t dbSNP:1048124
422 422 a, g dbSNP:368821483
424 424 g, t dbSNP:765825432
427 427 a, g dbSNP:1048134
428 428 c, g dbSNP:41555012
444 444 c, t dbSNP:707952
448 448 c, t dbSNP:751132570
451 451 c, t dbSNP:707951
455 455 c, t dbSNP:780889985
457 457 g, t dbSNP:750209003
460 460 a, g dbSNP:755849378
467 467 a, t dbSNP:779906935
493 493 a, c, g dbSNP:1048173
495 495 a, g dbSNP:777689650
499 499 a, g dbSNP:747148636
507 507 g, t dbSNP:771270725
511 511 cg, gt dbSNP:386699556
511 511 c, g, t dbSNP:707950
512 512 g, t dbSNP:41547417
515 515 a, c, g dbSNP:369452859
522 522 a, g dbSNP:763347786
535 535 c, g dbSNP:764703448
537 537 a, c, t dbSNP:41545514
539 539 a, c dbSNP:41552014
540 540 g, t dbSNP:41544114
558 558 a, t dbSNP:755797601
567 567 c, t dbSNP:766043000
572 572 a, g dbSNP:753712484
576 576 a, t dbSNP:754837184
578 578 a, g dbSNP:778972437
581 581 c, t dbSNP:41550317
587 587 a, g dbSNP:757353300
590 590 c, t dbSNP:707949
593 593 c, t dbSNP:746044970
602 602 g, t dbSNP:41561312
603 603 a, c dbSNP:7990
605 605 a, g dbSNP:749728161
607 607 g, t dbSNP:707963
612 612 g, t dbSNP:707962
626 626 g, t dbSNP:762316160
629 629 c, g dbSNP:766977165
631 631 a, g dbSNP:772733847
634 634 c, t dbSNP:760295892
635 635 g, t dbSNP:766092893
640 640 a, c dbSNP:2308889
644 644 a, g dbSNP:774878538
645 645 a, c dbSNP:754856407
646 646 a, c, t dbSNP:2308890
647 647 a, c, g dbSNP:2308891
664 664 a, c dbSNP:745985669
667 667 a, g dbSNP:756288178
671 671 a, c dbSNP:761792620
676 676 a, g dbSNP:767398076
683 683 aca, gcc dbSNP:386699558
683 683 a, g dbSNP:9272785
684 684 c, t dbSNP:368763112
685 685 a, c dbSNP:3188543
690 690 c, t dbSNP:752846382
705 705 a, g dbSNP:144882737
714 714 g, t dbSNP:764392438
718 718 c, t dbSNP:1048372
719 719 a, c, g dbSNP:35087390
724 724 c, g dbSNP:746400068
726 726 a, g dbSNP:755621273
727 727 a, g dbSNP:1048381
738 738 a, g dbSNP:748903287
743 743 a, g dbSNP:9260
746 746 g, t dbSNP:41545416
753 753 c, t dbSNP:747964407
758 758 a, g dbSNP:772030388
759 759 a, g dbSNP:144742185
760 760 c, g dbSNP:1048414
763 763 c, t dbSNP:1048419
769 769 c, g dbSNP:1048430
775 775 a, c dbSNP:763137732
777 777 a, g dbSNP:1048439
777 777 a, g dbSNP:9272793
784 784 a, g dbSNP:751948582
785 785 c, t dbSNP:757506993
786 786 a, g dbSNP:767929297
791 791 a, g dbSNP:750856952
794 794 a, g dbSNP:756714305
811 811 a, g dbSNP:13379
814 814 a, g dbSNP:748852050
816 816 c, t dbSNP:754676254
817 817 a, c, g dbSNP:1130116
820 820 a, g dbSNP:771694885
827 827 c, t dbSNP:777651266
828 828 c, t dbSNP:746898931
834 834 -, g dbSNP:745533419
835 835 a, g dbSNP:3188642
838 838 -, ggg dbSNP:769393523
838 838 a, g, t dbSNP:1130117
839 839 a, g dbSNP:28400568
844 844 a, g dbSNP:1130151
851 851 c, t dbSNP:780437372
855 855 a, t dbSNP:1130152
856 856 a, c dbSNP:1130153
858 858 -, g dbSNP:141823983
860 860 c, g dbSNP:1130155
861 861 a, t dbSNP:1138373
869 869 a, g dbSNP:1142447
877 877 a, g dbSNP:1130156
881 881 a, g dbSNP:1130158
885 885 a, c, g, t dbSNP:9492
903 903 a, c dbSNP:1130161
906 906 c, g dbSNP:1130162
912 912 a, t dbSNP:9272932
921 921 a, t dbSNP:1048726
930 930 c, t dbSNP:9272934
938 938 c, t dbSNP:1065036
943 943 a, t dbSNP:1064985
949 949 a, c dbSNP:9272937
963 963 c, t dbSNP:28376734
965 965 c, t dbSNP:9272938
977 977 a, g dbSNP:707947
983 983 g, t dbSNP:192684527
990 990 a, c dbSNP:9272940
992 992 c, t dbSNP:9272941
993 993 a, g dbSNP:3208169
1001 1001 g, t dbSNP:9272943
1003 1003 a, t dbSNP:36216730
1007 1007 g, t dbSNP:707946
1008 1008 g, t dbSNP:28538060
1009 1009 a, g dbSNP:540435913
1010 1010 c, g, t dbSNP:707945
1016 1016 c, t dbSNP:9272948
1017 1017 a, t dbSNP:707944
1026 1026 g, t dbSNP:9272950
1030 1030 a, g dbSNP:183747744
1031 1031 c, t dbSNP:62404112
1034 1034 a, c dbSNP:9272951
1036 1036 c, t dbSNP:9272952
1037 1037 a, c, g dbSNP:9272953
1044 1044 a, c dbSNP:9272954
1054 1054 a, t dbSNP:9272955
1056 1056 g, t dbSNP:9272956
1061 1061 a, g dbSNP:9272957
1079 1079 a, c dbSNP:9272958
1085 1085 c, g dbSNP:1064991
1090 1090 a, g dbSNP:1064993
1104 1104 c, t dbSNP:1064994
1106 1106 -, tgt dbSNP:201571916
1107 1107 a, g dbSNP:539042680
1110 1110 g, t dbSNP:140118665

Target ORF information:

RefSeq Version XM_005274953
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ alpha 1 (HLA-DQA1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005274953

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGATCCTAA ACAAAGCTCT GATGCTGGGG GCCCTCGCCC TGACCACCGT GATGAGCCCT 
TGTGGAGGTG AAGACATTGT GGCTGACCAC GTTGCCTCTT ACGGTGTAAA CTTGTACCAG
TCTTACGGTC CCTCTGGCCA GTTCACCCAT GAATTTGATG GAGACGAGGA GTTCTATGTG
GACCTGGAGA GGAAGGAGAC TGTCTGGAAG TTGCCTCTGT TCCACAGACT TAGATTTGAC
CCGCAATTTG CACTGACAAA CATCGCTGTG CTAAAACATA ACTTGAACAT CCTGATTAAA
CGCTCCAACT CTACCGCTGC TACCAATGAG GTTCCTGAGG TCACAGTGTT TTCCAAGTCT
CCCGTGACAC TGGGTCAGCC CAACACCCTC ATCTGTCTTG TGGACAACAT CTTTCCTCCT
GTGGTCAACA TCACCTGGCT GAGCAATGGG CACTCAGTCA CAGAAGGTGT TTCTGAGACC
AGCTTCCTCT CCAAGAGTGA TCATTCCTTC TTCAAGATCA GTTACCTCAC CTTCCTCCCT
TCTGCTGATG AGATTTATGA CTGCAAGGTG GAGCACTGGG GCCTGGATGA GCCTCTTCTG
AAACACTGGG AGCCTGAGAT TCCAGCACCT ATGTCAGAGC TCACAGAGAC TGTGGTCTGT
GCCCTGGGGT TGTCTGTGGG CCTCGTGGGC ATTGTGGTGG GGACCGTCTT GATCATCCGA
GGCCTGCGTT CAGTTGGTGC TTCCAGACAC CAAGGGCCCT TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58237
Accession Version XM_011547645.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 624bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product HLA class II histocompatibility antigen, DQ alpha 1 chain isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167245.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGATCCTAA ACAAAGCTCT GATGCTGGGG GCCCTCGCCC TGACCACCGT GATGAGCCCT 
TGTGGAGGTG AAGACATTGT GGCTGACCAC GTTGCCTCTT ACGGTGTAAA CTTGTACCAG
TCTTACGGTC CCTCTGGCCA GTTCACCCAT GAATTTGATG GAGACGAGGA GTTCTATGTG
GACCTGGAGA GGAAGGAGAC TGTCTGGAAG TTGCCTCTGT TCCACAGACT TAGATTTGAC
CCGCAATTTG CACTGACAAA CATCGCTGTG CTAAAACATA ACTTGAACAT CCTGATTAAA
CGCTCCAACT CTACCGCTGC TACCAATGAG GTTCCTGAGG TCACAGTGTT TTCCAAGTCT
CCCGTGACAC TGGGTCAGCC CAACACCCTC ATCTGTCTTG TGGACAACAT CTTTCCTCCT
GTGGTCAACA TCACCTGGCT GAGCAATGGG CACTCAGTCA CAGAAGGTGT TTCTGAGACC
AGCTTCCTCT CCAAGAGTGA TCATTCCTTC TTCAAGATCA GTTACCTCAC CTTCCTCCCT
TCTGCTGATG AGATTTATGA CTGCAAGGTG GAGCACTGGG GCCTGGATGA GCCTCTTCTG
AAACACTGGG GTGTTACCTA CTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011545947.1
CDS59..682
Misc Feature(1)143..385(+)
Misc Feature(2)389..667(+)
Misc Feature(3)401..583(+)
Misc Feature(4)473..583(+)
Translation
Position Chain Variation Link
1 1 c, t dbSNP:9272425
8 8 a, t dbSNP:751610786
9 9 a, c, g dbSNP:761951737
11 11 a, t dbSNP:750601509
12 12 a, c, g, t dbSNP:9272426
13 13 c, t dbSNP:755486767
15 15 c, g, t dbSNP:779603527
18 18 a, c, t dbSNP:771761512
19 19 a, g dbSNP:746602382
20 20 a, g dbSNP:3207966
22 22 a, t dbSNP:776466842
25 25 g, t dbSNP:759291994
27 27 a, g dbSNP:200004305
30 30 a, c, g dbSNP:3187964
31 31 a, c dbSNP:767518956
33 33 a, g dbSNP:773197867
39 39 a, g dbSNP:1047985
42 42 a, g dbSNP:766556976
43 43 c, t dbSNP:754198500
45 45 g, t dbSNP:755460098
55 55 a, g dbSNP:765825504
57 57 a, g dbSNP:28383345
61 61 a, g dbSNP:758975009
71 71 a, g dbSNP:777374378
75 75 c, t dbSNP:746521319
79 79 a, g dbSNP:756905853
80 80 a, c dbSNP:1047989
88 88 g, t dbSNP:745666028
89 89 a, g, t dbSNP:1047992
91 91 a, c, t dbSNP:749185114
94 94 c, g, t dbSNP:1047993
97 97 a, c, g, t dbSNP:1130034
98 98 c, g dbSNP:776775819
105 105 c, t dbSNP:369177390
106 106 c, t dbSNP:41551712
107 107 a, g dbSNP:12722039
108 108 c, t dbSNP:758923598
111 111 c, t dbSNP:11545686
114 114 c, g dbSNP:751079577
115 115 c, t dbSNP:756778447
118 118 c, t dbSNP:1129735
119 119 c, t dbSNP:745457579
128 128 a, g dbSNP:41545012
132 132 a, g dbSNP:12722042
136 136 a, t dbSNP:749132342
140 140 a, g dbSNP:373782144
148 148 c, t dbSNP:1129737
151 151 c, t dbSNP:1129738
153 153 c, t dbSNP:763996857
158 158 -, tg dbSNP:776891401
159 159 a, g dbSNP:1129740
160 160 -, aac dbSNP:746358168
160 160 c, t dbSNP:3205916
164 164 a, g dbSNP:780232299
166 166 a, g dbSNP:1129742
170 170 a, t dbSNP:749434805
172 172 a, c, g dbSNP:1129744
180 180 c, t dbSNP:1071630
184 184 c, t dbSNP:1129753
185 185 a, g dbSNP:772362984
189 189 c, t dbSNP:41549715
196 196 c, g dbSNP:1129759
201 201 a, t dbSNP:12722051
204 204 c, g dbSNP:1048023
216 216 a, g dbSNP:775981593
218 218 g, t dbSNP:759125166
219 219 a, g, t dbSNP:112029808
221 221 g, t dbSNP:775152680
222 222 a, c, g dbSNP:762518779
223 223 c, t dbSNP:1048027
227 227 c, g dbSNP:10093
234 234 a, t dbSNP:767607207
235 235 c, t dbSNP:1129765
246 246 a, g dbSNP:1142323
249 249 a, g dbSNP:36219699
250 250 g, t dbSNP:752894641
253 253 g, t dbSNP:758646527
257 257 a, g dbSNP:41543221
261 261 c, t dbSNP:1142324
266 266 a, c, t dbSNP:1142326
267 267 a, g dbSNP:3207983
268 268 g, t dbSNP:3207984
270 270 g, t dbSNP:1142328
275 275 -, ga dbSNP:760671632
275 275 c, g dbSNP:12722061
276 276 a, t dbSNP:3208105
277 277 g, t dbSNP:9272697
277 277 g, t dbSNP:546754435
278 278 -, tc dbSNP:764189141
278 278 a, c, t dbSNP:9272698
278 278 c, t dbSNP:568289532
279 279 -, cca dbSNP:763406304
281 281 -, ag dbSNP:767108045
281 281 a, c, t dbSNP:3207985
282 282 -, a dbSNP:200303565
282 282 a, g dbSNP:28383449
282 282 -, g dbSNP:772825532
283 283 a, c dbSNP:9272699
283 283 a, c dbSNP:568465013
284 284 -, gac dbSNP:765866325
284 284 a, c, g, t dbSNP:1048052
285 285 a, g dbSNP:12722069
287 287 c, t dbSNP:3188043
290 290 -, gg dbSNP:753442670
290 290 a, g, t dbSNP:4193
291 291 g, t dbSNP:758597345
296 296 g, t dbSNP:781416959
300 300 a, c, g, t dbSNP:41541412
304 304 a, g dbSNP:9272702
305 305 g, t dbSNP:1142331
306 306 g, t dbSNP:1142332
311 311 a, c dbSNP:761573168
312 312 c, t dbSNP:771830448
314 314 a, t dbSNP:772909319
315 315 c, g dbSNP:1142333
316 316 a, g dbSNP:766272167
322 322 a, c, g dbSNP:1142334
324 324 -, tg dbSNP:765228558
325 325 c, g, t dbSNP:1048491
326 326 -, tcc dbSNP:752397622
326 326 a, g dbSNP:764286168
328 328 -, gg dbSNP:758294111
328 328 c, g dbSNP:3188501
329 329 a, c, g, t dbSNP:1142335
330 330 -, ct dbSNP:777760029
330 330 c, t dbSNP:9272709
330 330 c, t dbSNP:527759771
332 332 a, g dbSNP:756616948
335 335 c, t dbSNP:780496366
337 337 a, g dbSNP:1048087
339 339 a, c dbSNP:754481952
341 341 c, g, t dbSNP:778568255
344 344 a, g dbSNP:771641715
347 347 -, g, gt, gtc dbSNP:9282026
348 348 g, t dbSNP:9279910
349 349 -, ca dbSNP:745963770
349 349 c, t dbSNP:36219014
350 350 -, a, gtc dbSNP:371894400
350 350 -, a dbSNP:199556640
350 350 a, c, g dbSNP:1064944
359 359 c, t dbSNP:41542116
360 360 g, t dbSNP:759480677
362 362 g, t dbSNP:764120909
363 363 a, c, g dbSNP:1129808
367 367 c, g dbSNP:767673676
373 373 c, t dbSNP:1142338
374 374 a, g dbSNP:142591955
377 377 g, t dbSNP:766867329
384 384 a, c dbSNP:201351192
393 393 c, g dbSNP:760756426
412 412 a, c dbSNP:771325735
417 417 c, t dbSNP:1048122
421 421 c, t dbSNP:1048124
422 422 a, g dbSNP:368821483
424 424 g, t dbSNP:765825432
427 427 a, g dbSNP:1048134
428 428 c, g dbSNP:41555012
444 444 c, t dbSNP:707952
448 448 c, t dbSNP:751132570
451 451 c, t dbSNP:707951
455 455 c, t dbSNP:780889985
457 457 g, t dbSNP:750209003
460 460 a, g dbSNP:755849378
467 467 a, t dbSNP:779906935
493 493 a, c, g dbSNP:1048173
495 495 a, g dbSNP:777689650
499 499 a, g dbSNP:747148636
507 507 g, t dbSNP:771270725
511 511 cg, gt dbSNP:386699556
511 511 c, g, t dbSNP:707950
512 512 g, t dbSNP:41547417
515 515 a, c, g dbSNP:369452859
522 522 a, g dbSNP:763347786
535 535 c, g dbSNP:764703448
537 537 a, c, t dbSNP:41545514
539 539 a, c dbSNP:41552014
540 540 g, t dbSNP:41544114
558 558 a, t dbSNP:755797601
567 567 c, t dbSNP:766043000
572 572 a, g dbSNP:753712484
576 576 a, t dbSNP:754837184
578 578 a, g dbSNP:778972437
581 581 c, t dbSNP:41550317
587 587 a, g dbSNP:757353300
590 590 c, t dbSNP:707949
593 593 c, t dbSNP:746044970
602 602 g, t dbSNP:41561312
603 603 a, c dbSNP:7990
605 605 a, g dbSNP:749728161
607 607 g, t dbSNP:707963
612 612 g, t dbSNP:707962
626 626 g, t dbSNP:762316160
629 629 c, g dbSNP:766977165
631 631 a, g dbSNP:772733847
634 634 c, t dbSNP:760295892
635 635 g, t dbSNP:766092893
640 640 a, c dbSNP:2308889
644 644 a, g dbSNP:774878538
645 645 a, c dbSNP:754856407
646 646 a, c, t dbSNP:2308890
647 647 a, c, g dbSNP:2308891
664 664 a, c dbSNP:745985669
667 667 a, g dbSNP:756288178
669 669 a, g dbSNP:1130151
676 676 c, t dbSNP:780437372
680 680 a, t dbSNP:1130152
681 681 a, c dbSNP:1130153
683 683 -, g dbSNP:141823983
685 685 c, g dbSNP:1130155
686 686 a, t dbSNP:1138373
694 694 a, g dbSNP:1142447
702 702 a, g dbSNP:1130156
706 706 a, g dbSNP:1130158
710 710 a, c, g, t dbSNP:9492
728 728 a, c dbSNP:1130161
731 731 c, g dbSNP:1130162
737 737 a, t dbSNP:9272932
746 746 a, t dbSNP:1048726
755 755 c, t dbSNP:9272934
763 763 c, t dbSNP:1065036
768 768 a, t dbSNP:1064985
774 774 a, c dbSNP:9272937
788 788 c, t dbSNP:28376734
790 790 c, t dbSNP:9272938
802 802 a, g dbSNP:707947
808 808 g, t dbSNP:192684527
815 815 a, c dbSNP:9272940
817 817 c, t dbSNP:9272941
818 818 a, g dbSNP:3208169
826 826 g, t dbSNP:9272943
828 828 a, t dbSNP:36216730
832 832 g, t dbSNP:707946
833 833 g, t dbSNP:28538060
834 834 a, g dbSNP:540435913
835 835 c, g, t dbSNP:707945
841 841 c, t dbSNP:9272948
842 842 a, t dbSNP:707944
851 851 g, t dbSNP:9272950
855 855 a, g dbSNP:183747744
856 856 c, t dbSNP:62404112
859 859 a, c dbSNP:9272951
861 861 c, t dbSNP:9272952
862 862 a, c, g dbSNP:9272953
869 869 a, c dbSNP:9272954
879 879 a, t dbSNP:9272955
881 881 g, t dbSNP:9272956
886 886 a, g dbSNP:9272957
904 904 a, c dbSNP:9272958
910 910 c, g dbSNP:1064991
915 915 a, g dbSNP:1064993
929 929 c, t dbSNP:1064994
931 931 -, tgt dbSNP:201571916
932 932 a, g dbSNP:539042680
935 935 g, t dbSNP:140118665

Target ORF information:

RefSeq Version XM_011547645
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ alpha 1 (HLA-DQA1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011547645

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGATCCTAA ACAAAGCTCT GATGCTGGGG GCCCTCGCCC TGACCACCGT GATGAGCCCT 
TGTGGAGGTG AAGACATTGT GGCTGACCAC GTTGCCTCTT ACGGTGTAAA CTTGTACCAG
TCTTACGGTC CCTCTGGCCA GTTCACCCAT GAATTTGATG GAGACGAGGA GTTCTATGTG
GACCTGGAGA GGAAGGAGAC TGTCTGGAAG TTGCCTCTGT TCCACAGACT TAGATTTGAC
CCGCAATTTG CACTGACAAA CATCGCTGTG CTAAAACATA ACTTGAACAT CCTGATTAAA
CGCTCCAACT CTACCGCTGC TACCAATGAG GTTCCTGAGG TCACAGTGTT TTCCAAGTCT
CCCGTGACAC TGGGTCAGCC CAACACCCTC ATCTGTCTTG TGGACAACAT CTTTCCTCCT
GTGGTCAACA TCACCTGGCT GAGCAATGGG CACTCAGTCA CAGAAGGTGT TTCTGAGACC
AGCTTCCTCT CCAAGAGTGA TCATTCCTTC TTCAAGATCA GTTACCTCAC CTTCCTCCCT
TCTGCTGATG AGATTTATGA CTGCAAGGTG GAGCACTGGG GCCTGGATGA GCCTCTTCTG
AAACACTGGG GTGTTACCTA CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu32852
Accession Version XM_005275108.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 765bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product HLA class II histocompatibility antigen, DQ alpha 1 chain isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167246.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578841636. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGATCCTAA ACAAAGCTCT GATGCTGGGG GCCCTCGCCC TGACCACCGT GATGAGCCCT 
TGTGGAGGTG AAGACATTGT GGCTGACCAC GTTGCCTCTT ACGGTGTAAA CTTGTACCAG
TCTTACGGTC CCTCTGGCCA GTTCACCCAT GAATTTGATG GAGACGAGGA GTTCTATGTG
GACCTGGAGA GGAAGGAGAC TGTCTGGAAG TTGCCTCTGT TCCACAGACT TAGATTTGAC
CCGCAATTTG CACTGACAAA CATCGCTGTG CTAAAACATA ACTTGAACAT CCTGATTAAA
CGCTCCAACT CTACCGCTGC TACCAATGAG GTTCCTGAGG TCACAGTGTT TTCCAAGTCT
CCCGTGACAC TGGGTCAGCC CAACACCCTC ATCTGTCTTG TGGACAACAT CTTTCCTCCT
GTGGTCAACA TCACCTGGCT GAGCAATGGG CACTCAGTCA CAGAAGGTGT TTCTGAGACC
AGCTTCCTCT CCAAGAGTGA TCATTCCTTC TTCAAGATCA GTTACCTCAC CTTCCTCCCT
TCTGCTGATG AGATTTATGA CTGCAAGGTG GAGCACTGGG GCCTGGATGA GCCTCTTCTG
AAACACTGGG AGCCTGAGAT TCCAGCACCT ATGTCAGAGC TCACAGAGAC TGTGGTCTGT
GCCCTGGGGT TGTCTGTGGG CCTCGTGGGC ATTGTGGTGG GGACCGTCTT GATCATCCGA
GGCCTGCGTT CAGTTGGTGC TTCCAGACAC CAAGGGCCCT TGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005275165.1
CDS59..823
Misc Feature(1)143..385(+)
Misc Feature(2)389..670(+)
Misc Feature(3)401..583(+)
Misc Feature(4)473..583(+)
Translation
Position Chain Variation Link
1 1 c, t dbSNP:9272425
8 8 a, t dbSNP:751610786
9 9 a, c, g dbSNP:761951737
11 11 a, t dbSNP:750601509
12 12 a, c, g, t dbSNP:9272426
13 13 c, t dbSNP:755486767
15 15 c, g, t dbSNP:779603527
18 18 a, c, t dbSNP:771761512
19 19 a, g dbSNP:746602382
20 20 a, g dbSNP:3207966
22 22 a, t dbSNP:776466842
25 25 g, t dbSNP:759291994
27 27 a, g dbSNP:200004305
30 30 a, c, g dbSNP:3187964
31 31 a, c dbSNP:767518956
33 33 a, g dbSNP:773197867
39 39 a, g dbSNP:1047985
42 42 a, g dbSNP:766556976
43 43 c, t dbSNP:754198500
45 45 g, t dbSNP:755460098
55 55 a, g dbSNP:765825504
57 57 a, g dbSNP:28383345
61 61 a, g dbSNP:758975009
71 71 a, g dbSNP:777374378
75 75 c, t dbSNP:746521319
79 79 a, g dbSNP:756905853
80 80 a, c dbSNP:1047989
88 88 g, t dbSNP:745666028
89 89 a, g, t dbSNP:1047992
91 91 a, c, t dbSNP:749185114
94 94 c, g, t dbSNP:1047993
97 97 a, c, g, t dbSNP:1130034
98 98 c, g dbSNP:776775819
105 105 c, t dbSNP:369177390
106 106 c, t dbSNP:41551712
107 107 a, g dbSNP:12722039
108 108 c, t dbSNP:758923598
111 111 c, t dbSNP:11545686
114 114 c, g dbSNP:751079577
115 115 c, t dbSNP:756778447
118 118 c, t dbSNP:1129735
119 119 c, t dbSNP:745457579
128 128 a, g dbSNP:41545012
132 132 a, g dbSNP:12722042
136 136 a, t dbSNP:749132342
140 140 a, g dbSNP:373782144
148 148 c, t dbSNP:1129737
151 151 c, t dbSNP:1129738
153 153 c, t dbSNP:763996857
158 158 -, tg dbSNP:776891401
159 159 a, g dbSNP:1129740
160 160 -, aac dbSNP:746358168
160 160 c, t dbSNP:3205916
164 164 a, g dbSNP:780232299
166 166 a, g dbSNP:1129742
170 170 a, t dbSNP:749434805
172 172 a, c, g dbSNP:1129744
180 180 c, t dbSNP:1071630
184 184 c, t dbSNP:1129753
185 185 a, g dbSNP:772362984
189 189 c, t dbSNP:41549715
196 196 c, g dbSNP:1129759
201 201 a, t dbSNP:12722051
204 204 c, g dbSNP:1048023
216 216 a, g dbSNP:775981593
218 218 g, t dbSNP:759125166
219 219 a, g, t dbSNP:112029808
221 221 g, t dbSNP:775152680
222 222 a, c, g dbSNP:762518779
223 223 c, t dbSNP:1048027
227 227 c, g dbSNP:10093
234 234 a, t dbSNP:767607207
235 235 c, t dbSNP:1129765
246 246 a, g dbSNP:1142323
249 249 a, g dbSNP:36219699
250 250 g, t dbSNP:752894641
253 253 g, t dbSNP:758646527
257 257 a, g dbSNP:41543221
261 261 c, t dbSNP:1142324
266 266 a, c, t dbSNP:1142326
267 267 a, g dbSNP:3207983
268 268 g, t dbSNP:3207984
270 270 g, t dbSNP:1142328
275 275 -, ga dbSNP:760671632
275 275 c, g dbSNP:12722061
276 276 a, t dbSNP:3208105
277 277 g, t dbSNP:9272697
277 277 g, t dbSNP:546754435
278 278 -, tc dbSNP:764189141
278 278 a, c, t dbSNP:9272698
278 278 c, t dbSNP:568289532
279 279 -, cca dbSNP:763406304
281 281 -, ag dbSNP:767108045
281 281 a, c, t dbSNP:3207985
282 282 -, a dbSNP:200303565
282 282 a, g dbSNP:28383449
282 282 -, g dbSNP:772825532
283 283 a, c dbSNP:9272699
283 283 a, c dbSNP:568465013
284 284 -, gac dbSNP:765866325
284 284 a, c, g, t dbSNP:1048052
285 285 a, g dbSNP:12722069
287 287 c, t dbSNP:3188043
290 290 -, gg dbSNP:753442670
290 290 a, g, t dbSNP:4193
291 291 g, t dbSNP:758597345
296 296 g, t dbSNP:781416959
300 300 a, c, g, t dbSNP:41541412
304 304 a, g dbSNP:9272702
305 305 g, t dbSNP:1142331
306 306 g, t dbSNP:1142332
311 311 a, c dbSNP:761573168
312 312 c, t dbSNP:771830448
314 314 a, t dbSNP:772909319
315 315 c, g dbSNP:1142333
316 316 a, g dbSNP:766272167
322 322 a, c, g dbSNP:1142334
324 324 -, tg dbSNP:765228558
325 325 c, g, t dbSNP:1048491
326 326 -, tcc dbSNP:752397622
326 326 a, g dbSNP:764286168
328 328 -, gg dbSNP:758294111
328 328 c, g dbSNP:3188501
329 329 a, c, g, t dbSNP:1142335
330 330 -, ct dbSNP:777760029
330 330 c, t dbSNP:9272709
330 330 c, t dbSNP:527759771
332 332 a, g dbSNP:756616948
335 335 c, t dbSNP:780496366
337 337 a, g dbSNP:1048087
339 339 a, c dbSNP:754481952
341 341 c, g, t dbSNP:778568255
344 344 a, g dbSNP:771641715
347 347 -, g, gt, gtc dbSNP:9282026
348 348 g, t dbSNP:9279910
349 349 -, ca dbSNP:745963770
349 349 c, t dbSNP:36219014
350 350 -, a, gtc dbSNP:371894400
350 350 -, a dbSNP:199556640
350 350 a, c, g dbSNP:1064944
359 359 c, t dbSNP:41542116
360 360 g, t dbSNP:759480677
362 362 g, t dbSNP:764120909
363 363 a, c, g dbSNP:1129808
367 367 c, g dbSNP:767673676
373 373 c, t dbSNP:1142338
374 374 a, g dbSNP:142591955
377 377 g, t dbSNP:766867329
384 384 a, c dbSNP:201351192
393 393 c, g dbSNP:760756426
412 412 a, c dbSNP:771325735
417 417 c, t dbSNP:1048122
421 421 c, t dbSNP:1048124
422 422 a, g dbSNP:368821483
424 424 g, t dbSNP:765825432
427 427 a, g dbSNP:1048134
428 428 c, g dbSNP:41555012
444 444 c, t dbSNP:707952
448 448 c, t dbSNP:751132570
451 451 c, t dbSNP:707951
455 455 c, t dbSNP:780889985
457 457 g, t dbSNP:750209003
460 460 a, g dbSNP:755849378
467 467 a, t dbSNP:779906935
493 493 a, c, g dbSNP:1048173
495 495 a, g dbSNP:777689650
499 499 a, g dbSNP:747148636
507 507 g, t dbSNP:771270725
511 511 cg, gt dbSNP:386699556
511 511 c, g, t dbSNP:707950
512 512 g, t dbSNP:41547417
515 515 a, c, g dbSNP:369452859
522 522 a, g dbSNP:763347786
535 535 c, g dbSNP:764703448
537 537 a, c, t dbSNP:41545514
539 539 a, c dbSNP:41552014
540 540 g, t dbSNP:41544114
558 558 a, t dbSNP:755797601
567 567 c, t dbSNP:766043000
572 572 a, g dbSNP:753712484
576 576 a, t dbSNP:754837184
578 578 a, g dbSNP:778972437
581 581 c, t dbSNP:41550317
587 587 a, g dbSNP:757353300
590 590 c, t dbSNP:707949
593 593 c, t dbSNP:746044970
602 602 g, t dbSNP:41561312
603 603 a, c dbSNP:7990
605 605 a, g dbSNP:749728161
607 607 g, t dbSNP:707963
612 612 g, t dbSNP:707962
626 626 g, t dbSNP:762316160
629 629 c, g dbSNP:766977165
631 631 a, g dbSNP:772733847
634 634 c, t dbSNP:760295892
635 635 g, t dbSNP:766092893
640 640 a, c dbSNP:2308889
644 644 a, g dbSNP:774878538
645 645 a, c dbSNP:754856407
646 646 a, c, t dbSNP:2308890
647 647 a, c, g dbSNP:2308891
664 664 a, c dbSNP:745985669
667 667 a, g dbSNP:756288178
671 671 a, c dbSNP:761792620
676 676 a, g dbSNP:767398076
683 683 aca, gcc dbSNP:386699558
683 683 a, g dbSNP:9272785
684 684 c, t dbSNP:368763112
685 685 a, c dbSNP:3188543
690 690 c, t dbSNP:752846382
705 705 a, g dbSNP:144882737
714 714 g, t dbSNP:764392438
718 718 c, t dbSNP:1048372
719 719 a, c, g dbSNP:35087390
724 724 c, g dbSNP:746400068
726 726 a, g dbSNP:755621273
727 727 a, g dbSNP:1048381
738 738 a, g dbSNP:748903287
743 743 a, g dbSNP:9260
746 746 g, t dbSNP:41545416
753 753 c, t dbSNP:747964407
758 758 a, g dbSNP:772030388
759 759 a, g dbSNP:144742185
760 760 c, g dbSNP:1048414
763 763 c, t dbSNP:1048419
769 769 c, g dbSNP:1048430
775 775 a, c dbSNP:763137732
777 777 a, g dbSNP:1048439
777 777 a, g dbSNP:9272793
784 784 a, g dbSNP:751948582
785 785 c, t dbSNP:757506993
786 786 a, g dbSNP:767929297
791 791 a, g dbSNP:750856952
794 794 a, g dbSNP:756714305
811 811 a, g dbSNP:13379
814 814 a, g dbSNP:748852050
816 816 c, t dbSNP:754676254
817 817 a, c, g dbSNP:1130116
820 820 a, g dbSNP:771694885
827 827 c, t dbSNP:777651266
828 828 c, t dbSNP:746898931
834 834 -, g dbSNP:745533419
835 835 a, g dbSNP:3188642
838 838 -, ggg dbSNP:769393523
838 838 a, g, t dbSNP:1130117
839 839 a, g dbSNP:28400568
844 844 a, g dbSNP:1130151
851 851 c, t dbSNP:780437372
855 855 a, t dbSNP:1130152
856 856 a, c dbSNP:1130153
858 858 -, g dbSNP:141823983
860 860 c, g dbSNP:1130155
861 861 a, t dbSNP:1138373
869 869 a, g dbSNP:1142447
877 877 a, g dbSNP:1130156
881 881 a, g dbSNP:1130158
885 885 a, c, g, t dbSNP:9492
903 903 a, c dbSNP:1130161
906 906 c, g dbSNP:1130162
912 912 a, t dbSNP:9272932
921 921 a, t dbSNP:1048726
930 930 c, t dbSNP:9272934
938 938 c, t dbSNP:1065036
943 943 a, t dbSNP:1064985
949 949 a, c dbSNP:9272937
963 963 c, t dbSNP:28376734
965 965 c, t dbSNP:9272938
977 977 a, g dbSNP:707947
983 983 g, t dbSNP:192684527
990 990 a, c dbSNP:9272940
992 992 c, t dbSNP:9272941
993 993 a, g dbSNP:3208169
1001 1001 g, t dbSNP:9272943
1003 1003 a, t dbSNP:36216730
1007 1007 g, t dbSNP:707946
1008 1008 g, t dbSNP:28538060
1009 1009 a, g dbSNP:540435913
1010 1010 c, g, t dbSNP:707945
1016 1016 c, t dbSNP:9272948
1017 1017 a, t dbSNP:707944
1026 1026 g, t dbSNP:9272950
1030 1030 a, g dbSNP:183747744
1031 1031 c, t dbSNP:62404112
1034 1034 a, c dbSNP:9272951
1036 1036 c, t dbSNP:9272952
1037 1037 a, c, g dbSNP:9272953
1044 1044 a, c dbSNP:9272954
1054 1054 a, t dbSNP:9272955
1056 1056 g, t dbSNP:9272956
1061 1061 a, g dbSNP:9272957
1079 1079 a, c dbSNP:9272958
1085 1085 c, g dbSNP:1064991
1090 1090 a, g dbSNP:1064993
1104 1104 c, t dbSNP:1064994
1106 1106 -, tgt dbSNP:201571916
1107 1107 a, g dbSNP:539042680
1110 1110 g, t dbSNP:140118665

Target ORF information:

RefSeq Version XM_005275108
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ alpha 1 (HLA-DQA1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005275108

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGATCCTAA ACAAAGCTCT GATGCTGGGG GCCCTCGCCC TGACCACCGT GATGAGCCCT 
TGTGGAGGTG AAGACATTGT GGCTGACCAC GTTGCCTCTT ACGGTGTAAA CTTGTACCAG
TCTTACGGTC CCTCTGGCCA GTTCACCCAT GAATTTGATG GAGACGAGGA GTTCTATGTG
GACCTGGAGA GGAAGGAGAC TGTCTGGAAG TTGCCTCTGT TCCACAGACT TAGATTTGAC
CCGCAATTTG CACTGACAAA CATCGCTGTG CTAAAACATA ACTTGAACAT CCTGATTAAA
CGCTCCAACT CTACCGCTGC TACCAATGAG GTTCCTGAGG TCACAGTGTT TTCCAAGTCT
CCCGTGACAC TGGGTCAGCC CAACACCCTC ATCTGTCTTG TGGACAACAT CTTTCCTCCT
GTGGTCAACA TCACCTGGCT GAGCAATGGG CACTCAGTCA CAGAAGGTGT TTCTGAGACC
AGCTTCCTCT CCAAGAGTGA TCATTCCTTC TTCAAGATCA GTTACCTCAC CTTCCTCCCT
TCTGCTGATG AGATTTATGA CTGCAAGGTG GAGCACTGGG GCCTGGATGA GCCTCTTCTG
AAACACTGGG AGCCTGAGAT TCCAGCACCT ATGTCAGAGC TCACAGAGAC TGTGGTCTGT
GCCCTGGGGT TGTCTGTGGG CCTCGTGGGC ATTGTGGTGG GGACCGTCTT GATCATCCGA
GGCCTGCGTT CAGTTGGTGC TTCCAGACAC CAAGGGCCCT TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58237
Accession Version XM_011547876.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 624bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product HLA class II histocompatibility antigen, DQ alpha 1 chain isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167246.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGATCCTAA ACAAAGCTCT GATGCTGGGG GCCCTCGCCC TGACCACCGT GATGAGCCCT 
TGTGGAGGTG AAGACATTGT GGCTGACCAC GTTGCCTCTT ACGGTGTAAA CTTGTACCAG
TCTTACGGTC CCTCTGGCCA GTTCACCCAT GAATTTGATG GAGACGAGGA GTTCTATGTG
GACCTGGAGA GGAAGGAGAC TGTCTGGAAG TTGCCTCTGT TCCACAGACT TAGATTTGAC
CCGCAATTTG CACTGACAAA CATCGCTGTG CTAAAACATA ACTTGAACAT CCTGATTAAA
CGCTCCAACT CTACCGCTGC TACCAATGAG GTTCCTGAGG TCACAGTGTT TTCCAAGTCT
CCCGTGACAC TGGGTCAGCC CAACACCCTC ATCTGTCTTG TGGACAACAT CTTTCCTCCT
GTGGTCAACA TCACCTGGCT GAGCAATGGG CACTCAGTCA CAGAAGGTGT TTCTGAGACC
AGCTTCCTCT CCAAGAGTGA TCATTCCTTC TTCAAGATCA GTTACCTCAC CTTCCTCCCT
TCTGCTGATG AGATTTATGA CTGCAAGGTG GAGCACTGGG GCCTGGATGA GCCTCTTCTG
AAACACTGGG GTGTTACCTA CTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011546178.1
CDS59..682
Misc Feature(1)143..385(+)
Misc Feature(2)389..667(+)
Misc Feature(3)401..583(+)
Misc Feature(4)473..583(+)
Translation
Position Chain Variation Link
1 1 c, t dbSNP:9272425
8 8 a, t dbSNP:751610786
9 9 a, c, g dbSNP:761951737
11 11 a, t dbSNP:750601509
12 12 a, c, g, t dbSNP:9272426
13 13 c, t dbSNP:755486767
15 15 c, g, t dbSNP:779603527
18 18 a, c, t dbSNP:771761512
19 19 a, g dbSNP:746602382
20 20 a, g dbSNP:3207966
22 22 a, t dbSNP:776466842
25 25 g, t dbSNP:759291994
27 27 a, g dbSNP:200004305
30 30 a, c, g dbSNP:3187964
31 31 a, c dbSNP:767518956
33 33 a, g dbSNP:773197867
39 39 a, g dbSNP:1047985
42 42 a, g dbSNP:766556976
43 43 c, t dbSNP:754198500
45 45 g, t dbSNP:755460098
55 55 a, g dbSNP:765825504
57 57 a, g dbSNP:28383345
61 61 a, g dbSNP:758975009
71 71 a, g dbSNP:777374378
75 75 c, t dbSNP:746521319
79 79 a, g dbSNP:756905853
80 80 a, c dbSNP:1047989
88 88 g, t dbSNP:745666028
89 89 a, g, t dbSNP:1047992
91 91 a, c, t dbSNP:749185114
94 94 c, g, t dbSNP:1047993
97 97 a, c, g, t dbSNP:1130034
98 98 c, g dbSNP:776775819
105 105 c, t dbSNP:369177390
106 106 c, t dbSNP:41551712
107 107 a, g dbSNP:12722039
108 108 c, t dbSNP:758923598
111 111 c, t dbSNP:11545686
114 114 c, g dbSNP:751079577
115 115 c, t dbSNP:756778447
118 118 c, t dbSNP:1129735
119 119 c, t dbSNP:745457579
128 128 a, g dbSNP:41545012
132 132 a, g dbSNP:12722042
136 136 a, t dbSNP:749132342
140 140 a, g dbSNP:373782144
148 148 c, t dbSNP:1129737
151 151 c, t dbSNP:1129738
153 153 c, t dbSNP:763996857
158 158 -, tg dbSNP:776891401
159 159 a, g dbSNP:1129740
160 160 -, aac dbSNP:746358168
160 160 c, t dbSNP:3205916
164 164 a, g dbSNP:780232299
166 166 a, g dbSNP:1129742
170 170 a, t dbSNP:749434805
172 172 a, c, g dbSNP:1129744
180 180 c, t dbSNP:1071630
184 184 c, t dbSNP:1129753
185 185 a, g dbSNP:772362984
189 189 c, t dbSNP:41549715
196 196 c, g dbSNP:1129759
201 201 a, t dbSNP:12722051
204 204 c, g dbSNP:1048023
216 216 a, g dbSNP:775981593
218 218 g, t dbSNP:759125166
219 219 a, g, t dbSNP:112029808
221 221 g, t dbSNP:775152680
222 222 a, c, g dbSNP:762518779
223 223 c, t dbSNP:1048027
227 227 c, g dbSNP:10093
234 234 a, t dbSNP:767607207
235 235 c, t dbSNP:1129765
246 246 a, g dbSNP:1142323
249 249 a, g dbSNP:36219699
250 250 g, t dbSNP:752894641
253 253 g, t dbSNP:758646527
257 257 a, g dbSNP:41543221
261 261 c, t dbSNP:1142324
266 266 a, c, t dbSNP:1142326
267 267 a, g dbSNP:3207983
268 268 g, t dbSNP:3207984
270 270 g, t dbSNP:1142328
275 275 -, ga dbSNP:760671632
275 275 c, g dbSNP:12722061
276 276 a, t dbSNP:3208105
277 277 g, t dbSNP:9272697
277 277 g, t dbSNP:546754435
278 278 -, tc dbSNP:764189141
278 278 a, c, t dbSNP:9272698
278 278 c, t dbSNP:568289532
279 279 -, cca dbSNP:763406304
281 281 -, ag dbSNP:767108045
281 281 a, c, t dbSNP:3207985
282 282 -, a dbSNP:200303565
282 282 a, g dbSNP:28383449
282 282 -, g dbSNP:772825532
283 283 a, c dbSNP:9272699
283 283 a, c dbSNP:568465013
284 284 -, gac dbSNP:765866325
284 284 a, c, g, t dbSNP:1048052
285 285 a, g dbSNP:12722069
287 287 c, t dbSNP:3188043
290 290 -, gg dbSNP:753442670
290 290 a, g, t dbSNP:4193
291 291 g, t dbSNP:758597345
296 296 g, t dbSNP:781416959
300 300 a, c, g, t dbSNP:41541412
304 304 a, g dbSNP:9272702
305 305 g, t dbSNP:1142331
306 306 g, t dbSNP:1142332
311 311 a, c dbSNP:761573168
312 312 c, t dbSNP:771830448
314 314 a, t dbSNP:772909319
315 315 c, g dbSNP:1142333
316 316 a, g dbSNP:766272167
322 322 a, c, g dbSNP:1142334
324 324 -, tg dbSNP:765228558
325 325 c, g, t dbSNP:1048491
326 326 -, tcc dbSNP:752397622
326 326 a, g dbSNP:764286168
328 328 -, gg dbSNP:758294111
328 328 c, g dbSNP:3188501
329 329 a, c, g, t dbSNP:1142335
330 330 -, ct dbSNP:777760029
330 330 c, t dbSNP:9272709
330 330 c, t dbSNP:527759771
332 332 a, g dbSNP:756616948
335 335 c, t dbSNP:780496366
337 337 a, g dbSNP:1048087
339 339 a, c dbSNP:754481952
341 341 c, g, t dbSNP:778568255
344 344 a, g dbSNP:771641715
347 347 -, g, gt, gtc dbSNP:9282026
348 348 g, t dbSNP:9279910
349 349 -, ca dbSNP:745963770
349 349 c, t dbSNP:36219014
350 350 -, a, gtc dbSNP:371894400
350 350 -, a dbSNP:199556640
350 350 a, c, g dbSNP:1064944
359 359 c, t dbSNP:41542116
360 360 g, t dbSNP:759480677
362 362 g, t dbSNP:764120909
363 363 a, c, g dbSNP:1129808
367 367 c, g dbSNP:767673676
373 373 c, t dbSNP:1142338
374 374 a, g dbSNP:142591955
377 377 g, t dbSNP:766867329
384 384 a, c dbSNP:201351192
393 393 c, g dbSNP:760756426
412 412 a, c dbSNP:771325735
417 417 c, t dbSNP:1048122
421 421 c, t dbSNP:1048124
422 422 a, g dbSNP:368821483
424 424 g, t dbSNP:765825432
427 427 a, g dbSNP:1048134
428 428 c, g dbSNP:41555012
444 444 c, t dbSNP:707952
448 448 c, t dbSNP:751132570
451 451 c, t dbSNP:707951
455 455 c, t dbSNP:780889985
457 457 g, t dbSNP:750209003
460 460 a, g dbSNP:755849378
467 467 a, t dbSNP:779906935
493 493 a, c, g dbSNP:1048173
495 495 a, g dbSNP:777689650
499 499 a, g dbSNP:747148636
507 507 g, t dbSNP:771270725
511 511 cg, gt dbSNP:386699556
511 511 c, g, t dbSNP:707950
512 512 g, t dbSNP:41547417
515 515 a, c, g dbSNP:369452859
522 522 a, g dbSNP:763347786
535 535 c, g dbSNP:764703448
537 537 a, c, t dbSNP:41545514
539 539 a, c dbSNP:41552014
540 540 g, t dbSNP:41544114
558 558 a, t dbSNP:755797601
567 567 c, t dbSNP:766043000
572 572 a, g dbSNP:753712484
576 576 a, t dbSNP:754837184
578 578 a, g dbSNP:778972437
581 581 c, t dbSNP:41550317
587 587 a, g dbSNP:757353300
590 590 c, t dbSNP:707949
593 593 c, t dbSNP:746044970
602 602 g, t dbSNP:41561312
603 603 a, c dbSNP:7990
605 605 a, g dbSNP:749728161
607 607 g, t dbSNP:707963
612 612 g, t dbSNP:707962
626 626 g, t dbSNP:762316160
629 629 c, g dbSNP:766977165
631 631 a, g dbSNP:772733847
634 634 c, t dbSNP:760295892
635 635 g, t dbSNP:766092893
640 640 a, c dbSNP:2308889
644 644 a, g dbSNP:774878538
645 645 a, c dbSNP:754856407
646 646 a, c, t dbSNP:2308890
647 647 a, c, g dbSNP:2308891
664 664 a, c dbSNP:745985669
667 667 a, g dbSNP:756288178
669 669 a, g dbSNP:1130151
676 676 c, t dbSNP:780437372
680 680 a, t dbSNP:1130152
681 681 a, c dbSNP:1130153
683 683 -, g dbSNP:141823983
685 685 c, g dbSNP:1130155
686 686 a, t dbSNP:1138373
694 694 a, g dbSNP:1142447
702 702 a, g dbSNP:1130156
706 706 a, g dbSNP:1130158
710 710 a, c, g, t dbSNP:9492
728 728 a, c dbSNP:1130161
731 731 c, g dbSNP:1130162
737 737 a, t dbSNP:9272932
746 746 a, t dbSNP:1048726
755 755 c, t dbSNP:9272934
763 763 c, t dbSNP:1065036
768 768 a, t dbSNP:1064985
774 774 a, c dbSNP:9272937
788 788 c, t dbSNP:28376734
790 790 c, t dbSNP:9272938
802 802 a, g dbSNP:707947
808 808 g, t dbSNP:192684527
815 815 a, c dbSNP:9272940
817 817 c, t dbSNP:9272941
818 818 a, g dbSNP:3208169
826 826 g, t dbSNP:9272943
828 828 a, t dbSNP:36216730
832 832 g, t dbSNP:707946
833 833 g, t dbSNP:28538060
834 834 a, g dbSNP:540435913
835 835 c, g, t dbSNP:707945
841 841 c, t dbSNP:9272948
842 842 a, t dbSNP:707944
851 851 g, t dbSNP:9272950
855 855 a, g dbSNP:183747744
856 856 c, t dbSNP:62404112
859 859 a, c dbSNP:9272951
861 861 c, t dbSNP:9272952
862 862 a, c, g dbSNP:9272953
869 869 a, c dbSNP:9272954
879 879 a, t dbSNP:9272955
881 881 g, t dbSNP:9272956
886 886 a, g dbSNP:9272957
904 904 a, c dbSNP:9272958
910 910 c, g dbSNP:1064991
915 915 a, g dbSNP:1064993
929 929 c, t dbSNP:1064994
931 931 -, tgt dbSNP:201571916
932 932 a, g dbSNP:539042680
935 935 g, t dbSNP:140118665

Target ORF information:

RefSeq Version XM_011547876
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ alpha 1 (HLA-DQA1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011547876

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGATCCTAA ACAAAGCTCT GATGCTGGGG GCCCTCGCCC TGACCACCGT GATGAGCCCT 
TGTGGAGGTG AAGACATTGT GGCTGACCAC GTTGCCTCTT ACGGTGTAAA CTTGTACCAG
TCTTACGGTC CCTCTGGCCA GTTCACCCAT GAATTTGATG GAGACGAGGA GTTCTATGTG
GACCTGGAGA GGAAGGAGAC TGTCTGGAAG TTGCCTCTGT TCCACAGACT TAGATTTGAC
CCGCAATTTG CACTGACAAA CATCGCTGTG CTAAAACATA ACTTGAACAT CCTGATTAAA
CGCTCCAACT CTACCGCTGC TACCAATGAG GTTCCTGAGG TCACAGTGTT TTCCAAGTCT
CCCGTGACAC TGGGTCAGCC CAACACCCTC ATCTGTCTTG TGGACAACAT CTTTCCTCCT
GTGGTCAACA TCACCTGGCT GAGCAATGGG CACTCAGTCA CAGAAGGTGT TTCTGAGACC
AGCTTCCTCT CCAAGAGTGA TCATTCCTTC TTCAAGATCA GTTACCTCAC CTTCCTCCCT
TCTGCTGATG AGATTTATGA CTGCAAGGTG GAGCACTGGG GCCTGGATGA GCCTCTTCTG
AAACACTGGG GTGTTACCTA CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu55023
Accession Version XM_003846468.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 768bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product HLA class II histocompatibility antigen, DQ alpha 1 chain isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167247.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578841861. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGATCCTAA ACAAAGCTCT GATGCTGGGG GCCCTCGCCC TGACCACCGT GATGAGCCCT 
TGTGGAGGTG AAGACATTGT GGCTGACCAT GTTGCCTCTT ACGGTGTAAA CTTGTACCAG
TCTTATGGTC CCTCTGGGCA GTACAGCCAT GAATTTGATG GAGACGAGGA GTTCTATGTG
GACCTGGAGA GGAAGGAGAC TGTCTGGCAG TTGCCTCTGT TCCGCAGATT TAGAAGATTT
GACCCGCAAT TTGCACTGAC AAACATCGCT GTGCTAAAAC ATAACTTGAA CATCGTGATT
AAACGCTCCA ACTCTACCGC TGCTACCAAT GAGGTTCCTG AGGTCACAGT GTTTTCCAAG
TCTCCCGTGA CACTGGGTCA GCCCAACACC CTCATCTGTC TTGTGGACAA CATCTTTCCT
CCTGTGGTCA ACATCACCTG GCTGAGCAAT GGGCACTCAG TCACAGAAGG TGTTTCTGAG
ACCAGCTTCC TCTCCAAGAG TGATCATTCC TTCTTCAAGA TCAGTTACCT CACCTTCCTC
CCTTCTGATG ATGAGATTTA TGACTGCAAG GTGGAGCACT GGGGCCTGGA TGAGCCTCTT
CTGAAACACT GGGAGCCTGA GATTCCAACA CCTATGTCAG AGCTCACAGA GACTGTGGTC
TGCGCCCTGG GGTTGTCTGT GGGCCTCGTG GGCATTGTGG TGGGGACCGT CTTGATCATC
CGAGGCCTGC GTTCAGTTGG TGCTTCCAGA CACCAAGGGC CCTTGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_003846516.1
CDS65..832
Misc Feature(1)149..394(+)
Misc Feature(2)398..679(+)
Misc Feature(3)410..592(+)
Misc Feature(4)440..643(+)
Misc Feature(5)482..592(+)
Translation
Position Chain Variation Link
2 2 c, t dbSNP:9272424
6 6 a, g dbSNP:567258915
7 7 c, t dbSNP:9272425
14 14 a, t dbSNP:751610786
15 15 a, c, g dbSNP:761951737
17 17 a, t dbSNP:750601509
18 18 a, c, g, t dbSNP:9272426
19 19 c, t dbSNP:755486767
21 21 c, g, t dbSNP:779603527
24 24 a, c, t dbSNP:771761512
25 25 a, g dbSNP:746602382
26 26 a, g dbSNP:3207966
28 28 a, t dbSNP:776466842
31 31 g, t dbSNP:759291994
33 33 a, g dbSNP:200004305
36 36 a, c, g dbSNP:3187964
37 37 a, c dbSNP:767518956
39 39 a, g dbSNP:773197867
45 45 a, g dbSNP:1047985
48 48 a, g dbSNP:766556976
49 49 c, t dbSNP:754198500
51 51 g, t dbSNP:755460098
61 61 a, g dbSNP:765825504
63 63 a, g dbSNP:28383345
67 67 a, g dbSNP:758975009
77 77 a, g dbSNP:777374378
81 81 c, t dbSNP:746521319
85 85 a, g dbSNP:756905853
86 86 a, c dbSNP:1047989
94 94 g, t dbSNP:745666028
95 95 a, g, t dbSNP:1047992
97 97 a, c, t dbSNP:749185114
100 100 c, g, t dbSNP:1047993
103 103 a, c, g, t dbSNP:1130034
104 104 c, g dbSNP:776775819
111 111 c, t dbSNP:369177390
112 112 c, t dbSNP:41551712
113 113 a, g dbSNP:12722039
114 114 c, t dbSNP:758923598
117 117 c, t dbSNP:11545686
120 120 c, g dbSNP:751079577
121 121 c, t dbSNP:756778447
124 124 c, t dbSNP:1129735
125 125 c, t dbSNP:745457579
134 134 a, g dbSNP:41545012
138 138 a, g dbSNP:12722042
142 142 a, t dbSNP:749132342
146 146 a, g dbSNP:373782144
154 154 c, t dbSNP:1129737
157 157 c, t dbSNP:1129738
159 159 c, t dbSNP:763996857
164 164 -, tg dbSNP:776891401
165 165 a, g dbSNP:1129740
166 166 -, aac dbSNP:746358168
166 166 c, t dbSNP:3205916
170 170 a, g dbSNP:780232299
172 172 a, g dbSNP:1129742
176 176 a, t dbSNP:749434805
178 178 a, c, g dbSNP:1129744
186 186 c, t dbSNP:1071630
190 190 c, t dbSNP:1129753
191 191 a, g dbSNP:772362984
195 195 c, t dbSNP:41549715
202 202 c, g dbSNP:1129759
207 207 a, t dbSNP:12722051
210 210 c, g dbSNP:1048023
222 222 a, g dbSNP:775981593
224 224 g, t dbSNP:759125166
225 225 a, g, t dbSNP:112029808
227 227 g, t dbSNP:775152680
228 228 a, c, g dbSNP:762518779
229 229 c, t dbSNP:1048027
233 233 c, g dbSNP:10093
240 240 a, t dbSNP:767607207
241 241 c, t dbSNP:1129765
252 252 a, g dbSNP:1142323
255 255 a, g dbSNP:36219699
256 256 g, t dbSNP:752894641
259 259 g, t dbSNP:758646527
263 263 a, g dbSNP:41543221
267 267 c, t dbSNP:1142324
272 272 a, c, t dbSNP:1142326
273 273 a, g dbSNP:3207983
274 274 g, t dbSNP:3207984
276 276 g, t dbSNP:1142328
281 281 -, ga dbSNP:760671632
281 281 c, g dbSNP:12722061
282 282 a, t dbSNP:3208105
283 283 g, t dbSNP:9272697
283 283 g, t dbSNP:546754435
284 284 -, tc dbSNP:764189141
284 284 a, c, t dbSNP:9272698
284 284 c, t dbSNP:568289532
285 285 -, cca dbSNP:763406304
287 287 -, ag dbSNP:767108045
287 287 a, c, t dbSNP:3207985
288 288 -, a dbSNP:200303565
288 288 a, g dbSNP:28383449
288 288 -, g dbSNP:772825532
289 289 a, c dbSNP:9272699
289 289 a, c dbSNP:568465013
290 290 -, gac dbSNP:765866325
290 290 a, c, g, t dbSNP:1048052
291 291 a, g dbSNP:12722069
293 293 c, t dbSNP:3188043
296 296 -, gg dbSNP:753442670
296 296 a, g, t dbSNP:4193
297 297 g, t dbSNP:758597345
299 299 -, ggt dbSNP:534654325
299 299 a, g dbSNP:12722072
300 300 a, g dbSNP:751915559
300 300 -, g dbSNP:41556812
301 301 a, t dbSNP:12722074
305 305 g, t dbSNP:781416959
309 309 a, c, g, t dbSNP:41541412
313 313 a, g dbSNP:9272702
314 314 g, t dbSNP:1142331
315 315 g, t dbSNP:1142332
320 320 a, c dbSNP:761573168
321 321 c, t dbSNP:771830448
323 323 a, t dbSNP:772909319
324 324 c, g dbSNP:1142333
325 325 a, g dbSNP:766272167
331 331 a, c, g dbSNP:1142334
333 333 -, tg dbSNP:765228558
334 334 c, g, t dbSNP:1048491
335 335 -, tcc dbSNP:752397622
335 335 a, g dbSNP:764286168
337 337 -, gg dbSNP:758294111
337 337 c, g dbSNP:3188501
338 338 a, c, g, t dbSNP:1142335
339 339 -, ct dbSNP:777760029
339 339 c, t dbSNP:9272709
339 339 c, t dbSNP:527759771
341 341 a, g dbSNP:756616948
344 344 c, t dbSNP:780496366
346 346 a, g dbSNP:1048087
348 348 a, c dbSNP:754481952
350 350 c, g, t dbSNP:778568255
353 353 a, g dbSNP:771641715
356 356 -, g, gt, gtc dbSNP:9282026
357 357 g, t dbSNP:9279910
358 358 -, ca dbSNP:745963770
358 358 c, t dbSNP:36219014
359 359 -, a, gtc dbSNP:371894400
359 359 -, a dbSNP:199556640
359 359 a, c, g dbSNP:1064944
368 368 c, t dbSNP:41542116
369 369 g, t dbSNP:759480677
371 371 g, t dbSNP:764120909
372 372 a, c, g dbSNP:1129808
376 376 c, g dbSNP:767673676
382 382 c, t dbSNP:1142338
383 383 a, g dbSNP:142591955
386 386 g, t dbSNP:766867329
393 393 a, c dbSNP:201351192
402 402 c, g dbSNP:760756426
421 421 a, c dbSNP:771325735
426 426 c, t dbSNP:1048122
430 430 c, t dbSNP:1048124
431 431 a, g dbSNP:368821483
433 433 g, t dbSNP:765825432
436 436 a, g dbSNP:1048134
437 437 c, g dbSNP:41555012
453 453 c, t dbSNP:707952
457 457 c, t dbSNP:751132570
460 460 c, t dbSNP:707951
464 464 c, t dbSNP:780889985
466 466 g, t dbSNP:750209003
469 469 a, g dbSNP:755849378
476 476 a, t dbSNP:779906935
502 502 a, c, g dbSNP:1048173
504 504 a, g dbSNP:777689650
508 508 a, g dbSNP:747148636
516 516 g, t dbSNP:771270725
520 520 cg, gt dbSNP:386699556
520 520 c, g, t dbSNP:707950
521 521 g, t dbSNP:41547417
524 524 a, c, g dbSNP:369452859
531 531 a, g dbSNP:763347786
544 544 c, g dbSNP:764703448
546 546 a, c, t dbSNP:41545514
548 548 a, c dbSNP:41552014
549 549 g, t dbSNP:41544114
567 567 a, t dbSNP:755797601
576 576 c, t dbSNP:766043000
581 581 a, g dbSNP:753712484
585 585 a, t dbSNP:754837184
587 587 a, g dbSNP:778972437
590 590 c, t dbSNP:41550317
596 596 a, g dbSNP:757353300
599 599 c, t dbSNP:707949
602 602 c, t dbSNP:746044970
611 611 g, t dbSNP:41561312
612 612 a, c dbSNP:7990
614 614 a, g dbSNP:749728161
616 616 g, t dbSNP:707963
621 621 g, t dbSNP:707962
635 635 g, t dbSNP:762316160
638 638 c, g dbSNP:766977165
640 640 a, g dbSNP:772733847
643 643 c, t dbSNP:760295892
644 644 g, t dbSNP:766092893
649 649 a, c dbSNP:2308889
653 653 a, g dbSNP:774878538
654 654 a, c dbSNP:754856407
655 655 a, c, t dbSNP:2308890
656 656 a, c, g dbSNP:2308891
673 673 a, c dbSNP:745985669
676 676 a, g dbSNP:756288178
680 680 a, c dbSNP:761792620
685 685 a, g dbSNP:767398076
692 692 aca, gcc dbSNP:386699558
692 692 a, g dbSNP:9272785
693 693 c, t dbSNP:368763112
694 694 a, c dbSNP:3188543
699 699 c, t dbSNP:752846382
714 714 a, g dbSNP:144882737
723 723 g, t dbSNP:764392438
727 727 c, t dbSNP:1048372
728 728 a, c, g dbSNP:35087390
733 733 c, g dbSNP:746400068
735 735 a, g dbSNP:755621273
736 736 a, g dbSNP:1048381
747 747 a, g dbSNP:748903287
752 752 a, g dbSNP:9260
755 755 g, t dbSNP:41545416
762 762 c, t dbSNP:747964407
767 767 a, g dbSNP:772030388
768 768 a, g dbSNP:144742185
769 769 c, g dbSNP:1048414
772 772 c, t dbSNP:1048419
778 778 c, g dbSNP:1048430
784 784 a, c dbSNP:763137732
786 786 a, g dbSNP:1048439
786 786 a, g dbSNP:9272793
793 793 a, g dbSNP:751948582
794 794 c, t dbSNP:757506993
795 795 a, g dbSNP:767929297
800 800 a, g dbSNP:750856952
803 803