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HLA-DQB2 major histocompatibility complex, class II, DQ beta 2 [Homo sapiens (human)]

Gene Symbol HLA-DQB2
Entrez Gene ID 3120
Full Name major histocompatibility complex, class II, DQ beta 2
Synonyms HLA-DQB1, HLA-DXB
General protein information
Preferred Names
HLA class II histocompatibility antigen, DQ beta 2 chain
Names
HLA class II histocompatibility antigen, DQ beta 2 chain
MHC class II antigen DQB2
major histocompatibility complex, class II, DQ beta 1
HLA class II histocompatibility antigen, DX beta chain
DV19.1 (major histocompatibility complex, class II, DQ beta 2 (HLA-DXB))
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p21

Summary HLA-DQB2 belongs to the family of HLA class II beta chain paralogs. Class II molecules are heterodimers consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. They play a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). Polymorphisms in the alpha and beta chains specify the peptide binding specificity, and typing for these polymorphisms is routinely done for bone marrow transplantation. However this gene, HLA-DQB2, is not routinely typed, as it is not thought to have an effect on transplantation. There is conflicting evidence in the literature and public sequence databases for the protein-coding capacity of HLA-DQB2. Because there is evidence of transcription and an intact ORF, HLA-DQB2 is represented in Entrez Gene and in RefSeq as a protein-coding locus. [provided by RefSeq, Oct 2010]. lac of sum
Disorder MIM:

615161

Disorder Html:





The following HLA-DQB2 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HLA-DQB2 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu40289 XM_005249051 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu58238 XM_011514560 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu58239 XM_011514561 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu54618 XM_005274855 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu58240 XM_011546309 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu58241 XM_011546310 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu54618 XM_005272807 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu58240 XM_011547242 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu58241 XM_011547243 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu58242 XM_011547646 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu58243 XM_011547647 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu58244 XM_011547648 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu58242 XM_011547877 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu58243 XM_011547878 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu58244 XM_011547879 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu58242 XM_011548045 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu58243 XM_011548046 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu58244 XM_011548047 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu58242 XM_011548233 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu58243 XM_011548234 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu58244 XM_011548235 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu40289 XM_005275546 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu58238 XM_011548425 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu58239 XM_011548426 PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu20438 NM_001198858 Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $269
OHu58245 NM_001300790 Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu40289
Accession Version XM_005249051.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 828bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product HLA class II histocompatibility antigen, DQ beta 2 chain isoform X13
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007592.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578811645. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCTCTGC AGATCCCTGG AGGCTTTTGG GCAGCAGCTG TGACCGTGAT GCTGGTGATG 
CTGAGCACCC CAGTGGCTGA GGCCAGAGAC TTTCCCAAGG ATTTCTTGGT CCAGTTTAAG
GGCATGTGCT ACTTCACCAA CGGGACAGAG CGCGTGCGCG GTGTGGCCAG ATACATCTAT
AACCGCGAGG AGTACGGGCG CTTCGACAGC GACGTTGGGG AGTTCCAGGC GGTGACCGAG
CTGGGGCGGA GCATCGAGGA CTGGAACAAC TATAAGGACT TCTTGGAGCA GGAGCGGGCC
GCGGTGGACA AGGTGTGCAG ACACAACTAC GAGGCGGAGC TGCGCACGAC CTTGCAGCGG
CAAGTGGAGC CCACAGTGAC CATCTCCCCA TCCAGGACAG AGGCCCTCAA CCACCACAAC
CTGCTGGTCT GCTCGGTGAC AGATTTCTAT CCAGCCCAGA TCAAAGTCCG GTGGTTTCGG
AATGACCAGG AGGAGACAGC CGGTGTTGTG TCCACCTCCC TCATTAGGAA TGGTGACTGG
ACCTTCCAGA TTCTGGTGAT GCTGGAAATA ACTCCCCAGC GTGGAGACAT CTACACCTGC
CAAGTGGAGC ACCCCAGCCT CCAGAGCCCC ATCACCGTGG AGTGGCGGGC TCAGTCTGAA
TCTGCCCAGA GCAAGATGCT GAGTGGCATT GGAGGCTTCG TGCTGGGGCT GATCTTCCTC
GGGCTGGGCC TTATCATCCG TCACAGGGGT CAGAAAGGAC CTCGAGGGCC TCCACCAGCA
GGTAATATTT CAGCCATGAT CCAGTCAGGG GAGAGGGCAC AGGCATAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005249108.1
CDS81..908
Misc Feature(1)201..413(+)
Misc Feature(2)450..731(+)
Misc Feature(3)465..629(+)
Misc Feature(4)504..722(+)
Misc Feature(5)528..626(+)
Translation
Position Chain Variation Link
15 15 a, t dbSNP:9276585
21 21 c, t dbSNP:781031809
70 70 c, g, t dbSNP:1573649
71 71 a, g dbSNP:777452739
76 76 a, g dbSNP:79638337
87 87 c, g dbSNP:202238720
89 89 a, g dbSNP:765751400
91 91 a, g dbSNP:759973134
96 96 c, t dbSNP:754341479
98 98 c, t dbSNP:766714258
100 100 c, g dbSNP:761067146
108 108 c, g, t dbSNP:201573322
112 112 c, t dbSNP:200239097
117 117 a, g dbSNP:767726406
120 120 c, g dbSNP:766009346
123 123 a, c dbSNP:572119983
126 126 a, g dbSNP:774505906
136 136 c, t dbSNP:201992526
141 141 c, t dbSNP:768852088
143 143 a, g dbSNP:745856868
146 146 a, c dbSNP:150473207
148 148 c, t dbSNP:770672954
152 152 a, g dbSNP:201129947
163 163 c, g dbSNP:200126619
187 187 c, t dbSNP:764317349
188 188 a, g dbSNP:763095415
189 189 g, t dbSNP:776465671
198 198 a, c dbSNP:770853395
201 201 g, t dbSNP:746793422
222 222 c, g dbSNP:772996134
227 227 a, c dbSNP:771853861
231 231 c, t dbSNP:747859033
234 234 c, g dbSNP:778517822
239 239 c, g, t dbSNP:762815
241 241 c, g dbSNP:780434886
246 246 a, g dbSNP:756573872
255 255 a, t dbSNP:529206424
263 263 c, t dbSNP:768051532
268 268 a, g dbSNP:115793818
275 275 c, t dbSNP:751869503
279 279 c, g dbSNP:764372793
280 280 a, g dbSNP:763148374
284 284 a, c dbSNP:752765802
289 289 g, t dbSNP:766235442
295 295 c, t dbSNP:764659256
296 296 c, t dbSNP:760736981
300 300 a, g dbSNP:773011968
304 304 a, t dbSNP:771906953
320 320 a, g dbSNP:147622494
322 322 c, t dbSNP:557758826
335 335 c, g dbSNP:774012292
352 352 a, g dbSNP:768235138
378 378 g, t dbSNP:748749283
407 407 -, c dbSNP:35282346
411 411 a, c, g dbSNP:201740281
413 413 a, g dbSNP:112964536
414 414 g, t dbSNP:200641501
415 415 c, t dbSNP:199858328
416 416 g, t dbSNP:746434568
417 417 g, t dbSNP:781754459
420 420 c, t dbSNP:77574565
422 422 a, c, g dbSNP:2071551
427 427 a, c dbSNP:751926608
428 428 g, t dbSNP:778155298
439 439 a, g dbSNP:762755845
440 440 a, g dbSNP:752861484
442 442 a, g dbSNP:200476184
451 451 c, t dbSNP:781770566
455 455 a, g dbSNP:771509689
456 456 a, g dbSNP:747382750
457 457 c, t dbSNP:778353470
469 469 a, c dbSNP:758680827
470 470 a, g dbSNP:753015252
479 479 a, g dbSNP:778974230
484 484 c, t dbSNP:755104751
491 491 c, t dbSNP:753854047
493 493 a, t dbSNP:541492094
495 495 a, c, t dbSNP:184113387
496 496 a, g dbSNP:763998181
500 500 c, t dbSNP:762622552
504 504 c, g dbSNP:775238559
507 507 a, g dbSNP:764749284
511 511 a, g dbSNP:759100922
515 515 a, g dbSNP:17850863
519 519 a, c dbSNP:770561591
529 529 a, g dbSNP:559050421
534 534 a, g dbSNP:747580825
535 535 c, g dbSNP:34554556
535 535 c, g dbSNP:773837565
540 540 a, g dbSNP:772529058
550 550 a, g dbSNP:1049110
558 558 a, c, t dbSNP:540615740
559 559 a, c, g dbSNP:576438613
560 560 g, t dbSNP:756164019
565 565 a, g dbSNP:56536369
566 566 c, t dbSNP:763896886
570 570 a, g dbSNP:758233735
579 579 a, g dbSNP:200716952
581 581 c, t dbSNP:200147031
582 582 a, g dbSNP:759329639
584 584 c, t dbSNP:201563919
585 585 a, g, t dbSNP:760233707
587 587 g, t dbSNP:773890444
593 593 c, t dbSNP:772397642
594 594 a, c dbSNP:748548031
595 595 c, t dbSNP:774640477
597 597 c, t dbSNP:769047510
600 600 c, t dbSNP:114145988
602 602 c, t dbSNP:780217889
608 608 a, g dbSNP:756215229
611 611 c, t dbSNP:745898010
615 615 a, g dbSNP:777781697
623 623 c, t dbSNP:758204528
629 629 g, t dbSNP:752675986
630 630 a, g dbSNP:778632746
631 631 g, t dbSNP:754800847
632 632 c, t dbSNP:753587946
636 636 a, g dbSNP:755542673
648 648 a, g dbSNP:766093798
650 650 a, g dbSNP:760288604
659 659 g, t dbSNP:749832708
660 660 c, t dbSNP:115645516
661 661 a, g dbSNP:762281871
665 665 a, c dbSNP:774897099
668 668 c, t dbSNP:769047010
669 669 a, g dbSNP:763261464
676 676 c, t dbSNP:775710959
677 677 c, t dbSNP:769944283
683 683 a, c dbSNP:745952197
687 687 c, g dbSNP:781195954
691 691 a, g dbSNP:771031206
696 696 a, g dbSNP:202167169
699 699 a, c, t dbSNP:754855896
706 706 a, g dbSNP:200827098
713 713 a, c dbSNP:779853574
715 715 c, t dbSNP:199585912
716 716 c, t dbSNP:750074923
717 717 a, g dbSNP:766982756
719 719 a, g dbSNP:761353450
723 723 a, t dbSNP:752035840
725 725 a, g dbSNP:764535884
726 726 c, t dbSNP:763320194
729 729 -, g dbSNP:776142583
734 734 a, g dbSNP:144961216
750 750 a, c dbSNP:556579358
751 751 a, g dbSNP:745625203
752 752 c, g dbSNP:780721175
754 754 a, t dbSNP:756866945
758 758 g, t dbSNP:746637959
759 759 c, g dbSNP:777323311
762 762 a, g dbSNP:9276572
767 767 ca, tg dbSNP:35354072
767 767 c, t dbSNP:9276571
768 768 a, g dbSNP:9276570
771 771 g, t dbSNP:755322600
780 780 a, g dbSNP:754178133
786 786 c, g dbSNP:766518048
788 788 a, g, t dbSNP:773541315
791 791 c, g dbSNP:34988824
800 800 c, t dbSNP:761941061
801 801 a, g, t dbSNP:769713525
806 806 a, g dbSNP:745655214
813 813 a, c dbSNP:776555504
815 815 c, t dbSNP:112126661
819 819 c, t dbSNP:746713265
820 820 a, g dbSNP:77504727
823 823 a, g dbSNP:771604184
824 824 a, c dbSNP:747612237
827 827 g, t dbSNP:779303788
828 828 a, g dbSNP:113761247
830 830 c, t dbSNP:754283114
833 833 g, t dbSNP:780512948
834 834 a, t dbSNP:756400541
841 841 c, g dbSNP:762911265
843 843 c, t dbSNP:566065650
857 857 a, t dbSNP:3213484
866 866 c, t dbSNP:760479732
884 884 g, t dbSNP:772927042
921 921 a, g dbSNP:771853713
930 930 c, g dbSNP:757479315
931 931 c, g, t dbSNP:773840220
932 932 a, g dbSNP:185581808
933 933 -, c dbSNP:775891466

Target ORF information:

RefSeq Version XM_005249051
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005249051

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCTCTGC AGATCCCTGG AGGCTTTTGG GCAGCAGCTG TGACCGTGAT GCTGGTGATG 
CTGAGCACCC CAGTGGCTGA GGCCAGAGAC TTTCCCAAGG ATTTCTTGGT CCAGTTTAAG
GGCATGTGCT ACTTCACCAA CGGGACAGAG CGCGTGCGCG GTGTGGCCAG ATACATCTAT
AACCGCGAGG AGTACGGGCG CTTCGACAGC GACGTTGGGG AGTTCCAGGC GGTGACCGAG
CTGGGGCGGA GCATCGAGGA CTGGAACAAC TATAAGGACT TCTTGGAGCA GGAGCGGGCC
GCGGTGGACA AGGTGTGCAG ACACAACTAC GAGGCGGAGC TGCGCACGAC CTTGCAGCGG
CAAGTGGAGC CCACAGTGAC CATCTCCCCA TCCAGGACAG AGGCCCTCAA CCACCACAAC
CTGCTGGTCT GCTCGGTGAC AGATTTCTAT CCAGCCCAGA TCAAAGTCCG GTGGTTTCGG
AATGACCAGG AGGAGACAGC CGGTGTTGTG TCCACCTCCC TCATTAGGAA TGGTGACTGG
ACCTTCCAGA TTCTGGTGAT GCTGGAAATA ACTCCCCAGC GTGGAGACAT CTACACCTGC
CAAGTGGAGC ACCCCAGCCT CCAGAGCCCC ATCACCGTGG AGTGGCGGGC TCAGTCTGAA
TCTGCCCAGA GCAAGATGCT GAGTGGCATT GGAGGCTTCG TGCTGGGGCT GATCTTCCTC
GGGCTGGGCC TTATCATCCG TCACAGGGGT CAGAAAGGAC CTCGAGGGCC TCCACCAGCA
GGTAATATTT CAGCCATGAT CCAGTCAGGG GAGAGGGCAC AGGCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58238
Accession Version XM_011514560.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 771bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product HLA class II histocompatibility antigen, DQ beta 2 chain isoform X14
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007592.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCTCTGC AGATCCCTGG AGGCTTTTGG GCAGCAGCTG TGACCGTGAT GCTGGTGATG 
CTGAGCACCC CAGTGGCTGA GGCCAGAGAC TTTCCCAAGG ATTTCTTGGT CCAGTTTAAG
GGCATGTGCT ACTTCACCAA CGGGACAGAG CGCGTGCGCG GTGTGGCCAG ATACATCTAT
AACCGCGAGG AGTACGGGCG CTTCGACAGC GACGTTGGGG AGTTCCAGGC GGTGACCGAG
CTGGGGCGGA GCATCGAGGA CTGGAACAAC TATAAGGACT TCTTGGAGCA GGAGCGGGCC
GCGGTGGACA AGGTGTGCAG ACACAACTAC GAGGCGGAGC TGCGCACGAC CTTGCAGCGG
CAAGTGGAGC CCACAGTGAC CATCTCCCCA TCCAGGACAG AGGCCCTCAA CCACCACAAC
CTGCTGGTCT GCTCGGTGAC AGATTTCTAT CCAGCCCAGA TCAAAGTCCG GTGGTTTCGG
AATGACCAGG AGGAGACAGC CGGTGTTGTG TCCACCTCCC TCATTAGGAA TGGTGACTGG
ACCTTCCAGA TTCTGGTGAT GCTGGAAATA ACTCCCCAGC GTGGAGACAT CTACACCTGC
CAAGTGGAGC ACCCCAGCCT CCAGAGCCCC ATCACCGTGG AGTGGCGGGC TCAGTCTGAA
TCTGCCCAGA GCAAGATGCT GAGTGGCATT GGAGGCTTCG TGCTGGGGCT GATCTTCCTC
GGGCTGGGCC TTATCATCCG TCACAGGGGT CAGAAAGGAC TCCTGCACTG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011512862.1
CDS80..850
Misc Feature(1)200..412(+)
Misc Feature(2)449..730(+)
Misc Feature(3)464..628(+)
Misc Feature(4)503..721(+)
Misc Feature(5)527..625(+)
Translation
Position Chain Variation Link
14 14 a, t dbSNP:9276585
20 20 c, t dbSNP:781031809
69 69 c, g, t dbSNP:1573649
70 70 a, g dbSNP:777452739
75 75 a, g dbSNP:79638337
86 86 c, g dbSNP:202238720
88 88 a, g dbSNP:765751400
90 90 a, g dbSNP:759973134
95 95 c, t dbSNP:754341479
97 97 c, t dbSNP:766714258
99 99 c, g dbSNP:761067146
107 107 c, g, t dbSNP:201573322
111 111 c, t dbSNP:200239097
116 116 a, g dbSNP:767726406
119 119 c, g dbSNP:766009346
122 122 a, c dbSNP:572119983
125 125 a, g dbSNP:774505906
135 135 c, t dbSNP:201992526
140 140 c, t dbSNP:768852088
142 142 a, g dbSNP:745856868
145 145 a, c dbSNP:150473207
147 147 c, t dbSNP:770672954
151 151 a, g dbSNP:201129947
162 162 c, g dbSNP:200126619
186 186 c, t dbSNP:764317349
187 187 a, g dbSNP:763095415
188 188 g, t dbSNP:776465671
197 197 a, c dbSNP:770853395
200 200 g, t dbSNP:746793422
221 221 c, g dbSNP:772996134
226 226 a, c dbSNP:771853861
230 230 c, t dbSNP:747859033
233 233 c, g dbSNP:778517822
238 238 c, g, t dbSNP:762815
240 240 c, g dbSNP:780434886
245 245 a, g dbSNP:756573872
254 254 a, t dbSNP:529206424
262 262 c, t dbSNP:768051532
267 267 a, g dbSNP:115793818
274 274 c, t dbSNP:751869503
278 278 c, g dbSNP:764372793
279 279 a, g dbSNP:763148374
283 283 a, c dbSNP:752765802
288 288 g, t dbSNP:766235442
294 294 c, t dbSNP:764659256
295 295 c, t dbSNP:760736981
299 299 a, g dbSNP:773011968
303 303 a, t dbSNP:771906953
319 319 a, g dbSNP:147622494
321 321 c, t dbSNP:557758826
334 334 c, g dbSNP:774012292
351 351 a, g dbSNP:768235138
377 377 g, t dbSNP:748749283
406 406 -, c dbSNP:35282346
410 410 a, c, g dbSNP:201740281
412 412 a, g dbSNP:112964536
413 413 g, t dbSNP:200641501
414 414 c, t dbSNP:199858328
415 415 g, t dbSNP:746434568
416 416 g, t dbSNP:781754459
419 419 c, t dbSNP:77574565
421 421 a, c, g dbSNP:2071551
426 426 a, c dbSNP:751926608
427 427 g, t dbSNP:778155298
438 438 a, g dbSNP:762755845
439 439 a, g dbSNP:752861484
441 441 a, g dbSNP:200476184
450 450 c, t dbSNP:781770566
454 454 a, g dbSNP:771509689
455 455 a, g dbSNP:747382750
456 456 c, t dbSNP:778353470
468 468 a, c dbSNP:758680827
469 469 a, g dbSNP:753015252
478 478 a, g dbSNP:778974230
483 483 c, t dbSNP:755104751
490 490 c, t dbSNP:753854047
492 492 a, t dbSNP:541492094
494 494 a, c, t dbSNP:184113387
495 495 a, g dbSNP:763998181
499 499 c, t dbSNP:762622552
503 503 c, g dbSNP:775238559
506 506 a, g dbSNP:764749284
510 510 a, g dbSNP:759100922
514 514 a, g dbSNP:17850863
518 518 a, c dbSNP:770561591
528 528 a, g dbSNP:559050421
533 533 a, g dbSNP:747580825
534 534 c, g dbSNP:34554556
534 534 c, g dbSNP:773837565
539 539 a, g dbSNP:772529058
549 549 a, g dbSNP:1049110
557 557 a, c, t dbSNP:540615740
558 558 a, c, g dbSNP:576438613
559 559 g, t dbSNP:756164019
564 564 a, g dbSNP:56536369
565 565 c, t dbSNP:763896886
569 569 a, g dbSNP:758233735
578 578 a, g dbSNP:200716952
580 580 c, t dbSNP:200147031
581 581 a, g dbSNP:759329639
583 583 c, t dbSNP:201563919
584 584 a, g, t dbSNP:760233707
586 586 g, t dbSNP:773890444
592 592 c, t dbSNP:772397642
593 593 a, c dbSNP:748548031
594 594 c, t dbSNP:774640477
596 596 c, t dbSNP:769047510
599 599 c, t dbSNP:114145988
601 601 c, t dbSNP:780217889
607 607 a, g dbSNP:756215229
610 610 c, t dbSNP:745898010
614 614 a, g dbSNP:777781697
622 622 c, t dbSNP:758204528
628 628 g, t dbSNP:752675986
629 629 a, g dbSNP:778632746
630 630 g, t dbSNP:754800847
631 631 c, t dbSNP:753587946
635 635 a, g dbSNP:755542673
647 647 a, g dbSNP:766093798
649 649 a, g dbSNP:760288604
658 658 g, t dbSNP:749832708
659 659 c, t dbSNP:115645516
660 660 a, g dbSNP:762281871
664 664 a, c dbSNP:774897099
667 667 c, t dbSNP:769047010
668 668 a, g dbSNP:763261464
675 675 c, t dbSNP:775710959
676 676 c, t dbSNP:769944283
682 682 a, c dbSNP:745952197
686 686 c, g dbSNP:781195954
690 690 a, g dbSNP:771031206
695 695 a, g dbSNP:202167169
698 698 a, c, t dbSNP:754855896
705 705 a, g dbSNP:200827098
712 712 a, c dbSNP:779853574
714 714 c, t dbSNP:199585912
715 715 c, t dbSNP:750074923
716 716 a, g dbSNP:766982756
718 718 a, g dbSNP:761353450
722 722 a, t dbSNP:752035840
724 724 a, g dbSNP:764535884
725 725 c, t dbSNP:763320194
728 728 -, g dbSNP:776142583
733 733 a, g dbSNP:144961216
749 749 a, c dbSNP:556579358
750 750 a, g dbSNP:745625203
751 751 c, g dbSNP:780721175
753 753 a, t dbSNP:756866945
757 757 g, t dbSNP:746637959
758 758 c, g dbSNP:777323311
761 761 a, g dbSNP:9276572
766 766 ca, tg dbSNP:35354072
766 766 c, t dbSNP:9276571
767 767 a, g dbSNP:9276570
770 770 g, t dbSNP:755322600
779 779 a, g dbSNP:754178133
785 785 c, g dbSNP:766518048
787 787 a, g, t dbSNP:773541315
790 790 c, g dbSNP:34988824
799 799 c, t dbSNP:761941061
800 800 a, g, t dbSNP:769713525
805 805 a, g dbSNP:745655214
812 812 a, c dbSNP:776555504
814 814 c, t dbSNP:112126661
818 818 c, t dbSNP:746713265
819 819 a, g dbSNP:77504727
822 822 a, g dbSNP:771604184
823 823 a, c dbSNP:747612237
826 826 g, t dbSNP:779303788
827 827 a, g dbSNP:113761247
829 829 c, t dbSNP:754283114
832 832 g, t dbSNP:780512948
833 833 a, t dbSNP:756400541
839 839 -, c dbSNP:765272140
856 856 a, g dbSNP:756114985
866 866 c, g dbSNP:750351833
891 891 g, t dbSNP:7774954
900 900 a, c dbSNP:761552899
901 901 c, t dbSNP:746579816
925 925 c, t dbSNP:561090370
933 933 g, t dbSNP:7774929
937 937 c, t dbSNP:6457646
944 944 c, g dbSNP:746156224
967 967 c, t dbSNP:190811600
982 982 -, ca dbSNP:777356205
1005 1005 c, t dbSNP:7774644
1019 1019 c, t dbSNP:9276558
1020 1020 a, g dbSNP:556703494
1022 1022 c, t dbSNP:145591506
1037 1037 c, t dbSNP:3656
1093 1093 a, g dbSNP:372075576
1097 1097 a, g dbSNP:149712668
1152 1152 a, g dbSNP:549347684
1163 1163 a, g dbSNP:7756516
1194 1194 a, g dbSNP:186400259

Target ORF information:

RefSeq Version XM_011514560
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011514560

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCTCTGC AGATCCCTGG AGGCTTTTGG GCAGCAGCTG TGACCGTGAT GCTGGTGATG 
CTGAGCACCC CAGTGGCTGA GGCCAGAGAC TTTCCCAAGG ATTTCTTGGT CCAGTTTAAG
GGCATGTGCT ACTTCACCAA CGGGACAGAG CGCGTGCGCG GTGTGGCCAG ATACATCTAT
AACCGCGAGG AGTACGGGCG CTTCGACAGC GACGTTGGGG AGTTCCAGGC GGTGACCGAG
CTGGGGCGGA GCATCGAGGA CTGGAACAAC TATAAGGACT TCTTGGAGCA GGAGCGGGCC
GCGGTGGACA AGGTGTGCAG ACACAACTAC GAGGCGGAGC TGCGCACGAC CTTGCAGCGG
CAAGTGGAGC CCACAGTGAC CATCTCCCCA TCCAGGACAG AGGCCCTCAA CCACCACAAC
CTGCTGGTCT GCTCGGTGAC AGATTTCTAT CCAGCCCAGA TCAAAGTCCG GTGGTTTCGG
AATGACCAGG AGGAGACAGC CGGTGTTGTG TCCACCTCCC TCATTAGGAA TGGTGACTGG
ACCTTCCAGA TTCTGGTGAT GCTGGAAATA ACTCCCCAGC GTGGAGACAT CTACACCTGC
CAAGTGGAGC ACCCCAGCCT CCAGAGCCCC ATCACCGTGG AGTGGCGGGC TCAGTCTGAA
TCTGCCCAGA GCAAGATGCT GAGTGGCATT GGAGGCTTCG TGCTGGGGCT GATCTTCCTC
GGGCTGGGCC TTATCATCCG TCACAGGGGT CAGAAAGGAC TCCTGCACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58239
Accession Version XM_011514561.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 660bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product HLA class II histocompatibility antigen, DQ beta 2 chain isoform X15
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007592.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGGCTCTGC AGATCCCTGG AGGCTTTTGG GCAGCAGCTG TGACCGTGAT GCTGGTGATG 
CTGAGCACCC CAGTGGCTGA GGCCAGAGAC TTTCCCAAGG ATTTCTTGGT CCAGTTTAAG
GGCATGTGCT ACTTCACCAA CGGGACAGAG CGCGTGCGCG GTGTGGCCAG ATACATCTAT
AACCGCGAGG AGTACGGGCG CTTCGACAGC GACGTTGGGG AGTTCCAGGC GGTGACCGAG
CTGGGGCGGA GCATCGAGGA CTGGAACAAC TATAAGGACT TCTTGGAGCA GGAGCGGGCC
GCGGTGGACA AGGTGTGCAG ACACAACTAC GAGGCGGAGC TGCGCACGAC CTTGCAGCGG
CAAGTGGAGC CCACAGTGAC CATCTCCCCA TCCAGGACAG AGGCCCTCAA CCACCACAAC
CTGCTGGTCT GCTCGGTGAC AGATTTCTAT CCAGCCCAGA TCAAAGTCCG GTGGTTTCGG
AATGACCAGG AGGAGACAGC CGGTGTTGTG TCCACCTCCC TCATTAGGAA TGGTGACTGG
ACCTTCCAGA TTCTGGTGAT GCTGGAAATA ACTCCCCAGC GTGGAGACAT CTACACCTGC
CAAGTGGAGC ACCCCAGCCT CCAGAGCCCC ATCACCGTGG AGTGGCGACT CCTGCACTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011512863.1
CDS81..740
Misc Feature(1)201..413(+)
Misc Feature(2)450..728(+)
Misc Feature(3)465..629(+)
Misc Feature(4)504..722(+)
Misc Feature(5)528..626(+)
Translation
Position Chain Variation Link
15 15 a, t dbSNP:9276585
21 21 c, t dbSNP:781031809
70 70 c, g, t dbSNP:1573649
71 71 a, g dbSNP:777452739
76 76 a, g dbSNP:79638337
87 87 c, g dbSNP:202238720
89 89 a, g dbSNP:765751400
91 91 a, g dbSNP:759973134
96 96 c, t dbSNP:754341479
98 98 c, t dbSNP:766714258
100 100 c, g dbSNP:761067146
108 108 c, g, t dbSNP:201573322
112 112 c, t dbSNP:200239097
117 117 a, g dbSNP:767726406
120 120 c, g dbSNP:766009346
123 123 a, c dbSNP:572119983
126 126 a, g dbSNP:774505906
136 136 c, t dbSNP:201992526
141 141 c, t dbSNP:768852088
143 143 a, g dbSNP:745856868
146 146 a, c dbSNP:150473207
148 148 c, t dbSNP:770672954
152 152 a, g dbSNP:201129947
163 163 c, g dbSNP:200126619
187 187 c, t dbSNP:764317349
188 188 a, g dbSNP:763095415
189 189 g, t dbSNP:776465671
198 198 a, c dbSNP:770853395
201 201 g, t dbSNP:746793422
222 222 c, g dbSNP:772996134
227 227 a, c dbSNP:771853861
231 231 c, t dbSNP:747859033
234 234 c, g dbSNP:778517822
239 239 c, g, t dbSNP:762815
241 241 c, g dbSNP:780434886
246 246 a, g dbSNP:756573872
255 255 a, t dbSNP:529206424
263 263 c, t dbSNP:768051532
268 268 a, g dbSNP:115793818
275 275 c, t dbSNP:751869503
279 279 c, g dbSNP:764372793
280 280 a, g dbSNP:763148374
284 284 a, c dbSNP:752765802
289 289 g, t dbSNP:766235442
295 295 c, t dbSNP:764659256
296 296 c, t dbSNP:760736981
300 300 a, g dbSNP:773011968
304 304 a, t dbSNP:771906953
320 320 a, g dbSNP:147622494
322 322 c, t dbSNP:557758826
335 335 c, g dbSNP:774012292
352 352 a, g dbSNP:768235138
378 378 g, t dbSNP:748749283
407 407 -, c dbSNP:35282346
411 411 a, c, g dbSNP:201740281
413 413 a, g dbSNP:112964536
414 414 g, t dbSNP:200641501
415 415 c, t dbSNP:199858328
416 416 g, t dbSNP:746434568
417 417 g, t dbSNP:781754459
420 420 c, t dbSNP:77574565
422 422 a, c, g dbSNP:2071551
427 427 a, c dbSNP:751926608
428 428 g, t dbSNP:778155298
439 439 a, g dbSNP:762755845
440 440 a, g dbSNP:752861484
442 442 a, g dbSNP:200476184
451 451 c, t dbSNP:781770566
455 455 a, g dbSNP:771509689
456 456 a, g dbSNP:747382750
457 457 c, t dbSNP:778353470
469 469 a, c dbSNP:758680827
470 470 a, g dbSNP:753015252
479 479 a, g dbSNP:778974230
484 484 c, t dbSNP:755104751
491 491 c, t dbSNP:753854047
493 493 a, t dbSNP:541492094
495 495 a, c, t dbSNP:184113387
496 496 a, g dbSNP:763998181
500 500 c, t dbSNP:762622552
504 504 c, g dbSNP:775238559
507 507 a, g dbSNP:764749284
511 511 a, g dbSNP:759100922
515 515 a, g dbSNP:17850863
519 519 a, c dbSNP:770561591
529 529 a, g dbSNP:559050421
534 534 a, g dbSNP:747580825
535 535 c, g dbSNP:34554556
535 535 c, g dbSNP:773837565
540 540 a, g dbSNP:772529058
550 550 a, g dbSNP:1049110
558 558 a, c, t dbSNP:540615740
559 559 a, c, g dbSNP:576438613
560 560 g, t dbSNP:756164019
565 565 a, g dbSNP:56536369
566 566 c, t dbSNP:763896886
570 570 a, g dbSNP:758233735
579 579 a, g dbSNP:200716952
581 581 c, t dbSNP:200147031
582 582 a, g dbSNP:759329639
584 584 c, t dbSNP:201563919
585 585 a, g, t dbSNP:760233707
587 587 g, t dbSNP:773890444
593 593 c, t dbSNP:772397642
594 594 a, c dbSNP:748548031
595 595 c, t dbSNP:774640477
597 597 c, t dbSNP:769047510
600 600 c, t dbSNP:114145988
602 602 c, t dbSNP:780217889
608 608 a, g dbSNP:756215229
611 611 c, t dbSNP:745898010
615 615 a, g dbSNP:777781697
623 623 c, t dbSNP:758204528
629 629 g, t dbSNP:752675986
630 630 a, g dbSNP:778632746
631 631 g, t dbSNP:754800847
632 632 c, t dbSNP:753587946
636 636 a, g dbSNP:755542673
648 648 a, g dbSNP:766093798
650 650 a, g dbSNP:760288604
659 659 g, t dbSNP:749832708
660 660 c, t dbSNP:115645516
661 661 a, g dbSNP:762281871
665 665 a, c dbSNP:774897099
668 668 c, t dbSNP:769047010
669 669 a, g dbSNP:763261464
676 676 c, t dbSNP:775710959
677 677 c, t dbSNP:769944283
683 683 a, c dbSNP:745952197
687 687 c, g dbSNP:781195954
691 691 a, g dbSNP:771031206
696 696 a, g dbSNP:202167169
699 699 a, c, t dbSNP:754855896
706 706 a, g dbSNP:200827098
713 713 a, c dbSNP:779853574
715 715 c, t dbSNP:199585912
716 716 c, t dbSNP:750074923
717 717 a, g dbSNP:766982756
719 719 a, g dbSNP:761353450
723 723 a, t dbSNP:752035840
725 725 a, g dbSNP:764535884
726 726 c, t dbSNP:763320194
729 729 -, c dbSNP:765272140
746 746 a, g dbSNP:756114985
756 756 c, g dbSNP:750351833
781 781 g, t dbSNP:7774954
790 790 a, c dbSNP:761552899
791 791 c, t dbSNP:746579816
815 815 c, t dbSNP:561090370
823 823 g, t dbSNP:7774929
827 827 c, t dbSNP:6457646
834 834 c, g dbSNP:746156224
857 857 c, t dbSNP:190811600
872 872 -, ca dbSNP:777356205
895 895 c, t dbSNP:7774644
909 909 c, t dbSNP:9276558
910 910 a, g dbSNP:556703494
912 912 c, t dbSNP:145591506
927 927 c, t dbSNP:3656
983 983 a, g dbSNP:372075576
987 987 a, g dbSNP:149712668
1042 1042 a, g dbSNP:549347684
1053 1053 a, g dbSNP:7756516
1084 1084 a, g dbSNP:186400259

Target ORF information:

RefSeq Version XM_011514561
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011514561

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCTCTGC AGATCCCTGG AGGCTTTTGG GCAGCAGCTG TGACCGTGAT GCTGGTGATG 
CTGAGCACCC CAGTGGCTGA GGCCAGAGAC TTTCCCAAGG ATTTCTTGGT CCAGTTTAAG
GGCATGTGCT ACTTCACCAA CGGGACAGAG CGCGTGCGCG GTGTGGCCAG ATACATCTAT
AACCGCGAGG AGTACGGGCG CTTCGACAGC GACGTTGGGG AGTTCCAGGC GGTGACCGAG
CTGGGGCGGA GCATCGAGGA CTGGAACAAC TATAAGGACT TCTTGGAGCA GGAGCGGGCC
GCGGTGGACA AGGTGTGCAG ACACAACTAC GAGGCGGAGC TGCGCACGAC CTTGCAGCGG
CAAGTGGAGC CCACAGTGAC CATCTCCCCA TCCAGGACAG AGGCCCTCAA CCACCACAAC
CTGCTGGTCT GCTCGGTGAC AGATTTCTAT CCAGCCCAGA TCAAAGTCCG GTGGTTTCGG
AATGACCAGG AGGAGACAGC CGGTGTTGTG TCCACCTCCC TCATTAGGAA TGGTGACTGG
ACCTTCCAGA TTCTGGTGAT GCTGGAAATA ACTCCCCAGC GTGGAGACAT CTACACCTGC
CAAGTGGAGC ACCCCAGCCT CCAGAGCCCC ATCACCGTGG AGTGGCGACT CCTGCACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu54618
Accession Version XM_005274855.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 828bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product HLA class II histocompatibility antigen, DQ beta 2 chain isoform X13
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167244.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578839601. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCTCTGC AGATCCCTGG AGGCTTTTGG GCAGCAGCTG TGACCGTGAT GCTGGTGATG 
CTGAGCACCC CAGTGGCTGA GGCCAGAGAC TTTCCCAAGG ATTTCTTGGT CCAGTTTAAG
GGCATGTGCT ACTTCACCAA CGGGACAGAG CGCGTGCGCG GTGTGGCCAG ATACATCTAT
AACCGCGAGG AGTACGGGCG CTTCGACAGC GACGTTGGGG AGTTCCAGGC GGTGACCGAG
CTGGGGCGGA GCATCGAGGA CTGGAACAAC TATAAGGACT TCTTGGAGCA GGAGCGGGCC
GCGGTGGACA AGGTGTGCAG ACACAACTAC GAGGCGGAGC TGCGCACGAC CTTGCAGCGG
CAAGTGGAGC CCACAGTGAC CATCTCCCCA TCCAGGACAG AGGCCCTCAA CCACCACAAC
CTGCTGGTCT GCTCAGTGAC AGATTTCTAT CCAGCCCAGA TCAAAGTCCG GTGGTTTCGG
AATGACCAGG AGGAGACAGC CGGTGTTGTG TCCACCTCCC TCATTAGGAA TGGTGACTGG
ACCTTCCAGA TTCTGGTGAT GCTGGAAATA ACTCCCCAGC GTGGAGACAT CTACACCTGC
CAAGTGGAGC ACCCCAGCCT CCAGAGCCCC ATCACCGTGG AGTGGCGGGC TCAGTCTGAA
TCTGCCCAGA GCAAGATGCT GAGTGGCATT GGAGGCTTCG TGCTGGGGCT GATCTTCCTC
GGGCTGGGCC TTATCATCCG TCACAGGGGT CAGAAAGGAC CTCGAGGGCC TCCACCAGCA
GGTAATATTT CAGCCATGAT CCAGTCAGGG GAGAGGGCAC AGGCATAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005274912.1
CDS63..890
Misc Feature(1)183..395(+)
Misc Feature(2)432..713(+)
Misc Feature(3)447..611(+)
Misc Feature(4)486..704(+)
Misc Feature(5)510..608(+)
Translation
Position Chain Variation Link
3 3 c, t dbSNP:781031809
52 52 c, g, t dbSNP:1573649
53 53 a, g dbSNP:777452739
58 58 a, g dbSNP:79638337
69 69 c, g dbSNP:202238720
71 71 a, g dbSNP:765751400
73 73 a, g dbSNP:759973134
78 78 c, t dbSNP:754341479
80 80 c, t dbSNP:766714258
82 82 c, g dbSNP:761067146
90 90 c, g, t dbSNP:201573322
94 94 c, t dbSNP:200239097
99 99 a, g dbSNP:767726406
102 102 c, g dbSNP:766009346
105 105 a, c dbSNP:572119983
108 108 a, g dbSNP:774505906
118 118 c, t dbSNP:201992526
123 123 c, t dbSNP:768852088
125 125 a, g dbSNP:745856868
128 128 a, c dbSNP:150473207
130 130 c, t dbSNP:770672954
134 134 a, g dbSNP:201129947
145 145 c, g dbSNP:200126619
169 169 c, t dbSNP:764317349
170 170 a, g dbSNP:763095415
171 171 g, t dbSNP:776465671
180 180 a, c dbSNP:770853395
183 183 g, t dbSNP:746793422
204 204 c, g dbSNP:772996134
209 209 a, c dbSNP:771853861
213 213 c, t dbSNP:747859033
216 216 c, g dbSNP:778517822
221 221 c, g, t dbSNP:762815
223 223 c, g dbSNP:780434886
228 228 a, g dbSNP:756573872
237 237 a, t dbSNP:529206424
245 245 c, t dbSNP:768051532
250 250 a, g dbSNP:115793818
257 257 c, t dbSNP:751869503
261 261 c, g dbSNP:764372793
262 262 a, g dbSNP:763148374
266 266 a, c dbSNP:752765802
271 271 g, t dbSNP:766235442
277 277 c, t dbSNP:764659256
278 278 c, t dbSNP:760736981
282 282 a, g dbSNP:773011968
286 286 a, t dbSNP:771906953
302 302 a, g dbSNP:147622494
304 304 c, t dbSNP:557758826
317 317 c, g dbSNP:774012292
334 334 a, g dbSNP:768235138
360 360 g, t dbSNP:748749283
389 389 -, c dbSNP:35282346
393 393 a, c, g dbSNP:201740281
395 395 a, g dbSNP:112964536
396 396 g, t dbSNP:200641501
397 397 c, t dbSNP:199858328
398 398 g, t dbSNP:746434568
399 399 g, t dbSNP:781754459
402 402 c, t dbSNP:77574565
404 404 a, c, g dbSNP:2071551
409 409 a, c dbSNP:751926608
410 410 g, t dbSNP:778155298
421 421 a, g dbSNP:762755845
422 422 a, g dbSNP:752861484
424 424 a, g dbSNP:200476184
433 433 c, t dbSNP:781770566
437 437 a, g dbSNP:771509689
438 438 a, g dbSNP:747382750
439 439 c, t dbSNP:778353470
451 451 a, c dbSNP:758680827
452 452 a, g dbSNP:753015252
461 461 a, g dbSNP:778974230
466 466 c, t dbSNP:755104751
473 473 c, t dbSNP:753854047
475 475 a, t dbSNP:541492094
477 477 a, c, t dbSNP:184113387
478 478 a, g dbSNP:763998181
482 482 c, t dbSNP:762622552
486 486 c, g dbSNP:775238559
489 489 a, g dbSNP:764749284
493 493 a, g dbSNP:759100922
497 497 a, g dbSNP:17850863
501 501 a, c dbSNP:770561591
511 511 a, g dbSNP:559050421
516 516 a, g dbSNP:747580825
517 517 c, g dbSNP:34554556
517 517 c, g dbSNP:773837565
522 522 a, g dbSNP:772529058
532 532 a, g dbSNP:1049110
540 540 a, c, t dbSNP:540615740
541 541 a, c, g dbSNP:576438613
542 542 g, t dbSNP:756164019
547 547 a, g dbSNP:56536369
548 548 c, t dbSNP:763896886
552 552 a, g dbSNP:758233735
561 561 a, g dbSNP:200716952
563 563 c, t dbSNP:200147031
564 564 a, g dbSNP:759329639
566 566 c, t dbSNP:201563919
567 567 a, g, t dbSNP:760233707
569 569 g, t dbSNP:773890444
575 575 c, t dbSNP:772397642
576 576 a, c dbSNP:748548031
577 577 c, t dbSNP:774640477
579 579 c, t dbSNP:769047510
582 582 c, t dbSNP:114145988
584 584 c, t dbSNP:780217889
590 590 a, g dbSNP:756215229
593 593 c, t dbSNP:745898010
597 597 a, g dbSNP:777781697
605 605 c, t dbSNP:758204528
611 611 g, t dbSNP:752675986
612 612 a, g dbSNP:778632746
613 613 g, t dbSNP:754800847
614 614 c, t dbSNP:753587946
618 618 a, g dbSNP:755542673
630 630 a, g dbSNP:766093798
632 632 a, g dbSNP:760288604
641 641 g, t dbSNP:749832708
642 642 c, t dbSNP:115645516
643 643 a, g dbSNP:762281871
647 647 a, c dbSNP:774897099
650 650 c, t dbSNP:769047010
651 651 a, g dbSNP:763261464
658 658 c, t dbSNP:775710959
659 659 c, t dbSNP:769944283
665 665 a, c dbSNP:745952197
669 669 c, g dbSNP:781195954
673 673 a, g dbSNP:771031206
678 678 a, g dbSNP:202167169
681 681 a, c, t dbSNP:754855896
688 688 a, g dbSNP:200827098
695 695 a, c dbSNP:779853574
697 697 c, t dbSNP:199585912
698 698 c, t dbSNP:750074923
699 699 a, g dbSNP:766982756
701 701 a, g dbSNP:761353450
705 705 a, t dbSNP:752035840
707 707 a, g dbSNP:764535884
708 708 c, t dbSNP:763320194
711 711 -, g dbSNP:776142583
716 716 a, g dbSNP:144961216
732 732 a, c dbSNP:556579358
733 733 a, g dbSNP:745625203
734 734 c, g dbSNP:780721175
736 736 a, t dbSNP:756866945
740 740 g, t dbSNP:746637959
741 741 c, g dbSNP:777323311
744 744 a, g dbSNP:9276572
749 749 ca, tg dbSNP:35354072
749 749 c, t dbSNP:9276571
750 750 a, g dbSNP:9276570
753 753 g, t dbSNP:755322600
762 762 a, g dbSNP:754178133
768 768 c, g dbSNP:766518048
770 770 a, g, t dbSNP:773541315
773 773 c, g dbSNP:34988824
782 782 c, t dbSNP:761941061
783 783 a, g, t dbSNP:769713525
788 788 a, g dbSNP:745655214
795 795 a, c dbSNP:776555504
797 797 c, t dbSNP:112126661
801 801 c, t dbSNP:746713265
802 802 a, g dbSNP:77504727
805 805 a, g dbSNP:771604184
806 806 a, c dbSNP:747612237
809 809 g, t dbSNP:779303788
810 810 a, g dbSNP:113761247
812 812 c, t dbSNP:754283114
815 815 g, t dbSNP:780512948
816 816 a, t dbSNP:756400541
823 823 c, g dbSNP:762911265
825 825 c, t dbSNP:566065650
839 839 a, t dbSNP:3213484
848 848 c, t dbSNP:760479732
866 866 g, t dbSNP:772927042
903 903 a, g dbSNP:771853713
912 912 c, g dbSNP:757479315
913 913 c, g, t dbSNP:773840220
914 914 a, g dbSNP:185581808
915 915 -, c dbSNP:775891466

Target ORF information:

RefSeq Version XM_005274855
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005274855

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCTCTGC AGATCCCTGG AGGCTTTTGG GCAGCAGCTG TGACCGTGAT GCTGGTGATG 
CTGAGCACCC CAGTGGCTGA GGCCAGAGAC TTTCCCAAGG ATTTCTTGGT CCAGTTTAAG
GGCATGTGCT ACTTCACCAA CGGGACAGAG CGCGTGCGCG GTGTGGCCAG ATACATCTAT
AACCGCGAGG AGTACGGGCG CTTCGACAGC GACGTTGGGG AGTTCCAGGC GGTGACCGAG
CTGGGGCGGA GCATCGAGGA CTGGAACAAC TATAAGGACT TCTTGGAGCA GGAGCGGGCC
GCGGTGGACA AGGTGTGCAG ACACAACTAC GAGGCGGAGC TGCGCACGAC CTTGCAGCGG
CAAGTGGAGC CCACAGTGAC CATCTCCCCA TCCAGGACAG AGGCCCTCAA CCACCACAAC
CTGCTGGTCT GCTCAGTGAC AGATTTCTAT CCAGCCCAGA TCAAAGTCCG GTGGTTTCGG
AATGACCAGG AGGAGACAGC CGGTGTTGTG TCCACCTCCC TCATTAGGAA TGGTGACTGG
ACCTTCCAGA TTCTGGTGAT GCTGGAAATA ACTCCCCAGC GTGGAGACAT CTACACCTGC
CAAGTGGAGC ACCCCAGCCT CCAGAGCCCC ATCACCGTGG AGTGGCGGGC TCAGTCTGAA
TCTGCCCAGA GCAAGATGCT GAGTGGCATT GGAGGCTTCG TGCTGGGGCT GATCTTCCTC
GGGCTGGGCC TTATCATCCG TCACAGGGGT CAGAAAGGAC CTCGAGGGCC TCCACCAGCA
GGTAATATTT CAGCCATGAT CCAGTCAGGG GAGAGGGCAC AGGCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58240
Accession Version XM_011546309.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 771bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product HLA class II histocompatibility antigen, DQ beta 2 chain isoform X14
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167244.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCTCTGC AGATCCCTGG AGGCTTTTGG GCAGCAGCTG TGACCGTGAT GCTGGTGATG 
CTGAGCACCC CAGTGGCTGA GGCCAGAGAC TTTCCCAAGG ATTTCTTGGT CCAGTTTAAG
GGCATGTGCT ACTTCACCAA CGGGACAGAG CGCGTGCGCG GTGTGGCCAG ATACATCTAT
AACCGCGAGG AGTACGGGCG CTTCGACAGC GACGTTGGGG AGTTCCAGGC GGTGACCGAG
CTGGGGCGGA GCATCGAGGA CTGGAACAAC TATAAGGACT TCTTGGAGCA GGAGCGGGCC
GCGGTGGACA AGGTGTGCAG ACACAACTAC GAGGCGGAGC TGCGCACGAC CTTGCAGCGG
CAAGTGGAGC CCACAGTGAC CATCTCCCCA TCCAGGACAG AGGCCCTCAA CCACCACAAC
CTGCTGGTCT GCTCAGTGAC AGATTTCTAT CCAGCCCAGA TCAAAGTCCG GTGGTTTCGG
AATGACCAGG AGGAGACAGC CGGTGTTGTG TCCACCTCCC TCATTAGGAA TGGTGACTGG
ACCTTCCAGA TTCTGGTGAT GCTGGAAATA ACTCCCCAGC GTGGAGACAT CTACACCTGC
CAAGTGGAGC ACCCCAGCCT CCAGAGCCCC ATCACCGTGG AGTGGCGGGC TCAGTCTGAA
TCTGCCCAGA GCAAGATGCT GAGTGGCATT GGAGGCTTCG TGCTGGGGCT GATCTTCCTC
GGGCTGGGCC TTATCATCCG TCACAGGGGT CAGAAAGGAC TCCTGCACTG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011544611.1
CDS63..833
Misc Feature(1)183..395(+)
Misc Feature(2)432..713(+)
Misc Feature(3)447..611(+)
Misc Feature(4)486..704(+)
Misc Feature(5)510..608(+)
Translation
Position Chain Variation Link
3 3 c, t dbSNP:781031809
52 52 c, g, t dbSNP:1573649
53 53 a, g dbSNP:777452739
58 58 a, g dbSNP:79638337
69 69 c, g dbSNP:202238720
71 71 a, g dbSNP:765751400
73 73 a, g dbSNP:759973134
78 78 c, t dbSNP:754341479
80 80 c, t dbSNP:766714258
82 82 c, g dbSNP:761067146
90 90 c, g, t dbSNP:201573322
94 94 c, t dbSNP:200239097
99 99 a, g dbSNP:767726406
102 102 c, g dbSNP:766009346
105 105 a, c dbSNP:572119983
108 108 a, g dbSNP:774505906
118 118 c, t dbSNP:201992526
123 123 c, t dbSNP:768852088
125 125 a, g dbSNP:745856868
128 128 a, c dbSNP:150473207
130 130 c, t dbSNP:770672954
134 134 a, g dbSNP:201129947
145 145 c, g dbSNP:200126619
169 169 c, t dbSNP:764317349
170 170 a, g dbSNP:763095415
171 171 g, t dbSNP:776465671
180 180 a, c dbSNP:770853395
183 183 g, t dbSNP:746793422
204 204 c, g dbSNP:772996134
209 209 a, c dbSNP:771853861
213 213 c, t dbSNP:747859033
216 216 c, g dbSNP:778517822
221 221 c, g, t dbSNP:762815
223 223 c, g dbSNP:780434886
228 228 a, g dbSNP:756573872
237 237 a, t dbSNP:529206424
245 245 c, t dbSNP:768051532
250 250 a, g dbSNP:115793818
257 257 c, t dbSNP:751869503
261 261 c, g dbSNP:764372793
262 262 a, g dbSNP:763148374
266 266 a, c dbSNP:752765802
271 271 g, t dbSNP:766235442
277 277 c, t dbSNP:764659256
278 278 c, t dbSNP:760736981
282 282 a, g dbSNP:773011968
286 286 a, t dbSNP:771906953
302 302 a, g dbSNP:147622494
304 304 c, t dbSNP:557758826
317 317 c, g dbSNP:774012292
334 334 a, g dbSNP:768235138
360 360 g, t dbSNP:748749283
389 389 -, c dbSNP:35282346
393 393 a, c, g dbSNP:201740281
395 395 a, g dbSNP:112964536
396 396 g, t dbSNP:200641501
397 397 c, t dbSNP:199858328
398 398 g, t dbSNP:746434568
399 399 g, t dbSNP:781754459
402 402 c, t dbSNP:77574565
404 404 a, c, g dbSNP:2071551
409 409 a, c dbSNP:751926608
410 410 g, t dbSNP:778155298
421 421 a, g dbSNP:762755845
422 422 a, g dbSNP:752861484
424 424 a, g dbSNP:200476184
433 433 c, t dbSNP:781770566
437 437 a, g dbSNP:771509689
438 438 a, g dbSNP:747382750
439 439 c, t dbSNP:778353470
451 451 a, c dbSNP:758680827
452 452 a, g dbSNP:753015252
461 461 a, g dbSNP:778974230
466 466 c, t dbSNP:755104751
473 473 c, t dbSNP:753854047
475 475 a, t dbSNP:541492094
477 477 a, c, t dbSNP:184113387
478 478 a, g dbSNP:763998181
482 482 c, t dbSNP:762622552
486 486 c, g dbSNP:775238559
489 489 a, g dbSNP:764749284
493 493 a, g dbSNP:759100922
497 497 a, g dbSNP:17850863
501 501 a, c dbSNP:770561591
511 511 a, g dbSNP:559050421
516 516 a, g dbSNP:747580825
517 517 c, g dbSNP:34554556
517 517 c, g dbSNP:773837565
522 522 a, g dbSNP:772529058
532 532 a, g dbSNP:1049110
540 540 a, c, t dbSNP:540615740
541 541 a, c, g dbSNP:576438613
542 542 g, t dbSNP:756164019
547 547 a, g dbSNP:56536369
548 548 c, t dbSNP:763896886
552 552 a, g dbSNP:758233735
561 561 a, g dbSNP:200716952
563 563 c, t dbSNP:200147031
564 564 a, g dbSNP:759329639
566 566 c, t dbSNP:201563919
567 567 a, g, t dbSNP:760233707
569 569 g, t dbSNP:773890444
575 575 c, t dbSNP:772397642
576 576 a, c dbSNP:748548031
577 577 c, t dbSNP:774640477
579 579 c, t dbSNP:769047510
582 582 c, t dbSNP:114145988
584 584 c, t dbSNP:780217889
590 590 a, g dbSNP:756215229
593 593 c, t dbSNP:745898010
597 597 a, g dbSNP:777781697
605 605 c, t dbSNP:758204528
611 611 g, t dbSNP:752675986
612 612 a, g dbSNP:778632746
613 613 g, t dbSNP:754800847
614 614 c, t dbSNP:753587946
618 618 a, g dbSNP:755542673
630 630 a, g dbSNP:766093798
632 632 a, g dbSNP:760288604
641 641 g, t dbSNP:749832708
642 642 c, t dbSNP:115645516
643 643 a, g dbSNP:762281871
647 647 a, c dbSNP:774897099
650 650 c, t dbSNP:769047010
651 651 a, g dbSNP:763261464
658 658 c, t dbSNP:775710959
659 659 c, t dbSNP:769944283
665 665 a, c dbSNP:745952197
669 669 c, g dbSNP:781195954
673 673 a, g dbSNP:771031206
678 678 a, g dbSNP:202167169
681 681 a, c, t dbSNP:754855896
688 688 a, g dbSNP:200827098
695 695 a, c dbSNP:779853574
697 697 c, t dbSNP:199585912
698 698 c, t dbSNP:750074923
699 699 a, g dbSNP:766982756
701 701 a, g dbSNP:761353450
705 705 a, t dbSNP:752035840
707 707 a, g dbSNP:764535884
708 708 c, t dbSNP:763320194
711 711 -, g dbSNP:776142583
716 716 a, g dbSNP:144961216
732 732 a, c dbSNP:556579358
733 733 a, g dbSNP:745625203
734 734 c, g dbSNP:780721175
736 736 a, t dbSNP:756866945
740 740 g, t dbSNP:746637959
741 741 c, g dbSNP:777323311
744 744 a, g dbSNP:9276572
749 749 ca, tg dbSNP:35354072
749 749 c, t dbSNP:9276571
750 750 a, g dbSNP:9276570
753 753 g, t dbSNP:755322600
762 762 a, g dbSNP:754178133
768 768 c, g dbSNP:766518048
770 770 a, g, t dbSNP:773541315
773 773 c, g dbSNP:34988824
782 782 c, t dbSNP:761941061
783 783 a, g, t dbSNP:769713525
788 788 a, g dbSNP:745655214
795 795 a, c dbSNP:776555504
797 797 c, t dbSNP:112126661
801 801 c, t dbSNP:746713265
802 802 a, g dbSNP:77504727
805 805 a, g dbSNP:771604184
806 806 a, c dbSNP:747612237
809 809 g, t dbSNP:779303788
810 810 a, g dbSNP:113761247
812 812 c, t dbSNP:754283114
815 815 g, t dbSNP:780512948
816 816 a, t dbSNP:756400541
822 822 -, c dbSNP:765272140
839 839 a, g dbSNP:756114985
849 849 c, g dbSNP:750351833
874 874 g, t dbSNP:7774954
883 883 a, c dbSNP:761552899
884 884 c, t dbSNP:746579816
908 908 c, t dbSNP:561090370
916 916 g, t dbSNP:7774929
920 920 c, t dbSNP:6457646
927 927 c, g dbSNP:746156224
950 950 c, t dbSNP:190811600
965 965 -, ca dbSNP:777356205
988 988 c, t dbSNP:7774644
1002 1002 c, t dbSNP:9276558
1003 1003 a, g dbSNP:556703494
1005 1005 c, t dbSNP:145591506
1020 1020 c, t dbSNP:3656
1076 1076 a, g dbSNP:372075576
1080 1080 a, g dbSNP:149712668
1135 1135 a, g dbSNP:549347684
1146 1146 a, g dbSNP:7756516
1177 1177 a, g dbSNP:186400259

Target ORF information:

RefSeq Version XM_011546309
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011546309

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCTCTGC AGATCCCTGG AGGCTTTTGG GCAGCAGCTG TGACCGTGAT GCTGGTGATG 
CTGAGCACCC CAGTGGCTGA GGCCAGAGAC TTTCCCAAGG ATTTCTTGGT CCAGTTTAAG
GGCATGTGCT ACTTCACCAA CGGGACAGAG CGCGTGCGCG GTGTGGCCAG ATACATCTAT
AACCGCGAGG AGTACGGGCG CTTCGACAGC GACGTTGGGG AGTTCCAGGC GGTGACCGAG
CTGGGGCGGA GCATCGAGGA CTGGAACAAC TATAAGGACT TCTTGGAGCA GGAGCGGGCC
GCGGTGGACA AGGTGTGCAG ACACAACTAC GAGGCGGAGC TGCGCACGAC CTTGCAGCGG
CAAGTGGAGC CCACAGTGAC CATCTCCCCA TCCAGGACAG AGGCCCTCAA CCACCACAAC
CTGCTGGTCT GCTCAGTGAC AGATTTCTAT CCAGCCCAGA TCAAAGTCCG GTGGTTTCGG
AATGACCAGG AGGAGACAGC CGGTGTTGTG TCCACCTCCC TCATTAGGAA TGGTGACTGG
ACCTTCCAGA TTCTGGTGAT GCTGGAAATA ACTCCCCAGC GTGGAGACAT CTACACCTGC
CAAGTGGAGC ACCCCAGCCT CCAGAGCCCC ATCACCGTGG AGTGGCGGGC TCAGTCTGAA
TCTGCCCAGA GCAAGATGCT GAGTGGCATT GGAGGCTTCG TGCTGGGGCT GATCTTCCTC
GGGCTGGGCC TTATCATCCG TCACAGGGGT CAGAAAGGAC TCCTGCACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58241
Accession Version XM_011546310.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 660bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product HLA class II histocompatibility antigen, DQ beta 2 chain isoform X15
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167244.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGGCTCTGC AGATCCCTGG AGGCTTTTGG GCAGCAGCTG TGACCGTGAT GCTGGTGATG 
CTGAGCACCC CAGTGGCTGA GGCCAGAGAC TTTCCCAAGG ATTTCTTGGT CCAGTTTAAG
GGCATGTGCT ACTTCACCAA CGGGACAGAG CGCGTGCGCG GTGTGGCCAG ATACATCTAT
AACCGCGAGG AGTACGGGCG CTTCGACAGC GACGTTGGGG AGTTCCAGGC GGTGACCGAG
CTGGGGCGGA GCATCGAGGA CTGGAACAAC TATAAGGACT TCTTGGAGCA GGAGCGGGCC
GCGGTGGACA AGGTGTGCAG ACACAACTAC GAGGCGGAGC TGCGCACGAC CTTGCAGCGG
CAAGTGGAGC CCACAGTGAC CATCTCCCCA TCCAGGACAG AGGCCCTCAA CCACCACAAC
CTGCTGGTCT GCTCAGTGAC AGATTTCTAT CCAGCCCAGA TCAAAGTCCG GTGGTTTCGG
AATGACCAGG AGGAGACAGC CGGTGTTGTG TCCACCTCCC TCATTAGGAA TGGTGACTGG
ACCTTCCAGA TTCTGGTGAT GCTGGAAATA ACTCCCCAGC GTGGAGACAT CTACACCTGC
CAAGTGGAGC ACCCCAGCCT CCAGAGCCCC ATCACCGTGG AGTGGCGACT CCTGCACTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011544612.1
CDS63..722
Misc Feature(1)183..395(+)
Misc Feature(2)432..710(+)
Misc Feature(3)447..611(+)
Misc Feature(4)486..704(+)
Misc Feature(5)510..608(+)
Translation
Position Chain Variation Link
3 3 c, t dbSNP:781031809
52 52 c, g, t dbSNP:1573649
53 53 a, g dbSNP:777452739
58 58 a, g dbSNP:79638337
69 69 c, g dbSNP:202238720
71 71 a, g dbSNP:765751400
73 73 a, g dbSNP:759973134
78 78 c, t dbSNP:754341479
80 80 c, t dbSNP:766714258
82 82 c, g dbSNP:761067146
90 90 c, g, t dbSNP:201573322
94 94 c, t dbSNP:200239097
99 99 a, g dbSNP:767726406
102 102 c, g dbSNP:766009346
105 105 a, c dbSNP:572119983
108 108 a, g dbSNP:774505906
118 118 c, t dbSNP:201992526
123 123 c, t dbSNP:768852088
125 125 a, g dbSNP:745856868
128 128 a, c dbSNP:150473207
130 130 c, t dbSNP:770672954
134 134 a, g dbSNP:201129947
145 145 c, g dbSNP:200126619
169 169 c, t dbSNP:764317349
170 170 a, g dbSNP:763095415
171 171 g, t dbSNP:776465671
180 180 a, c dbSNP:770853395
183 183 g, t dbSNP:746793422
204 204 c, g dbSNP:772996134
209 209 a, c dbSNP:771853861
213 213 c, t dbSNP:747859033
216 216 c, g dbSNP:778517822
221 221 c, g, t dbSNP:762815
223 223 c, g dbSNP:780434886
228 228 a, g dbSNP:756573872
237 237 a, t dbSNP:529206424
245 245 c, t dbSNP:768051532
250 250 a, g dbSNP:115793818
257 257 c, t dbSNP:751869503
261 261 c, g dbSNP:764372793
262 262 a, g dbSNP:763148374
266 266 a, c dbSNP:752765802
271 271 g, t dbSNP:766235442
277 277 c, t dbSNP:764659256
278 278 c, t dbSNP:760736981
282 282 a, g dbSNP:773011968
286 286 a, t dbSNP:771906953
302 302 a, g dbSNP:147622494
304 304 c, t dbSNP:557758826
317 317 c, g dbSNP:774012292
334 334 a, g dbSNP:768235138
360 360 g, t dbSNP:748749283
389 389 -, c dbSNP:35282346
393 393 a, c, g dbSNP:201740281
395 395 a, g dbSNP:112964536
396 396 g, t dbSNP:200641501
397 397 c, t dbSNP:199858328
398 398 g, t dbSNP:746434568
399 399 g, t dbSNP:781754459
402 402 c, t dbSNP:77574565
404 404 a, c, g dbSNP:2071551
409 409 a, c dbSNP:751926608
410 410 g, t dbSNP:778155298
421 421 a, g dbSNP:762755845
422 422 a, g dbSNP:752861484
424 424 a, g dbSNP:200476184
433 433 c, t dbSNP:781770566
437 437 a, g dbSNP:771509689
438 438 a, g dbSNP:747382750
439 439 c, t dbSNP:778353470
451 451 a, c dbSNP:758680827
452 452 a, g dbSNP:753015252
461 461 a, g dbSNP:778974230
466 466 c, t dbSNP:755104751
473 473 c, t dbSNP:753854047
475 475 a, t dbSNP:541492094
477 477 a, c, t dbSNP:184113387
478 478 a, g dbSNP:763998181
482 482 c, t dbSNP:762622552
486 486 c, g dbSNP:775238559
489 489 a, g dbSNP:764749284
493 493 a, g dbSNP:759100922
497 497 a, g dbSNP:17850863
501 501 a, c dbSNP:770561591
511 511 a, g dbSNP:559050421
516 516 a, g dbSNP:747580825
517 517 c, g dbSNP:34554556
517 517 c, g dbSNP:773837565
522 522 a, g dbSNP:772529058
532 532 a, g dbSNP:1049110
540 540 a, c, t dbSNP:540615740
541 541 a, c, g dbSNP:576438613
542 542 g, t dbSNP:756164019
547 547 a, g dbSNP:56536369
548 548 c, t dbSNP:763896886
552 552 a, g dbSNP:758233735
561 561 a, g dbSNP:200716952
563 563 c, t dbSNP:200147031
564 564 a, g dbSNP:759329639
566 566 c, t dbSNP:201563919
567 567 a, g, t dbSNP:760233707
569 569 g, t dbSNP:773890444
575 575 c, t dbSNP:772397642
576 576 a, c dbSNP:748548031
577 577 c, t dbSNP:774640477
579 579 c, t dbSNP:769047510
582 582 c, t dbSNP:114145988
584 584 c, t dbSNP:780217889
590 590 a, g dbSNP:756215229
593 593 c, t dbSNP:745898010
597 597 a, g dbSNP:777781697
605 605 c, t dbSNP:758204528
611 611 g, t dbSNP:752675986
612 612 a, g dbSNP:778632746
613 613 g, t dbSNP:754800847
614 614 c, t dbSNP:753587946
618 618 a, g dbSNP:755542673
630 630 a, g dbSNP:766093798
632 632 a, g dbSNP:760288604
641 641 g, t dbSNP:749832708
642 642 c, t dbSNP:115645516
643 643 a, g dbSNP:762281871
647 647 a, c dbSNP:774897099
650 650 c, t dbSNP:769047010
651 651 a, g dbSNP:763261464
658 658 c, t dbSNP:775710959
659 659 c, t dbSNP:769944283
665 665 a, c dbSNP:745952197
669 669 c, g dbSNP:781195954
673 673 a, g dbSNP:771031206
678 678 a, g dbSNP:202167169
681 681 a, c, t dbSNP:754855896
688 688 a, g dbSNP:200827098
695 695 a, c dbSNP:779853574
697 697 c, t dbSNP:199585912
698 698 c, t dbSNP:750074923
699 699 a, g dbSNP:766982756
701 701 a, g dbSNP:761353450
705 705 a, t dbSNP:752035840
707 707 a, g dbSNP:764535884
708 708 c, t dbSNP:763320194
711 711 -, c dbSNP:765272140
728 728 a, g dbSNP:756114985
738 738 c, g dbSNP:750351833
763 763 g, t dbSNP:7774954
772 772 a, c dbSNP:761552899
773 773 c, t dbSNP:746579816
797 797 c, t dbSNP:561090370
805 805 g, t dbSNP:7774929
809 809 c, t dbSNP:6457646
816 816 c, g dbSNP:746156224
839 839 c, t dbSNP:190811600
854 854 -, ca dbSNP:777356205
877 877 c, t dbSNP:7774644
891 891 c, t dbSNP:9276558
892 892 a, g dbSNP:556703494
894 894 c, t dbSNP:145591506
909 909 c, t dbSNP:3656
965 965 a, g dbSNP:372075576
969 969 a, g dbSNP:149712668
1024 1024 a, g dbSNP:549347684
1035 1035 a, g dbSNP:7756516
1066 1066 a, g dbSNP:186400259

Target ORF information:

RefSeq Version XM_011546310
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011546310

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCTCTGC AGATCCCTGG AGGCTTTTGG GCAGCAGCTG TGACCGTGAT GCTGGTGATG 
CTGAGCACCC CAGTGGCTGA GGCCAGAGAC TTTCCCAAGG ATTTCTTGGT CCAGTTTAAG
GGCATGTGCT ACTTCACCAA CGGGACAGAG CGCGTGCGCG GTGTGGCCAG ATACATCTAT
AACCGCGAGG AGTACGGGCG CTTCGACAGC GACGTTGGGG AGTTCCAGGC GGTGACCGAG
CTGGGGCGGA GCATCGAGGA CTGGAACAAC TATAAGGACT TCTTGGAGCA GGAGCGGGCC
GCGGTGGACA AGGTGTGCAG ACACAACTAC GAGGCGGAGC TGCGCACGAC CTTGCAGCGG
CAAGTGGAGC CCACAGTGAC CATCTCCCCA TCCAGGACAG AGGCCCTCAA CCACCACAAC
CTGCTGGTCT GCTCAGTGAC AGATTTCTAT CCAGCCCAGA TCAAAGTCCG GTGGTTTCGG
AATGACCAGG AGGAGACAGC CGGTGTTGTG TCCACCTCCC TCATTAGGAA TGGTGACTGG
ACCTTCCAGA TTCTGGTGAT GCTGGAAATA ACTCCCCAGC GTGGAGACAT CTACACCTGC
CAAGTGGAGC ACCCCAGCCT CCAGAGCCCC ATCACCGTGG AGTGGCGACT CCTGCACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu54618
Accession Version XM_005272807.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 828bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product HLA class II histocompatibility antigen, DQ beta 2 chain isoform X13
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_113891.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578840834. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCTCTGC AGATCCCTGG AGGCTTTTGG GCAGCAGCTG TGACCGTGAT GCTGGTGATG 
CTGAGCACCC CAGTGGCTGA GGCCAGAGAC TTTCCCAAGG ATTTCTTGGT CCAGTTTAAG
GGCATGTGCT ACTTCACCAA CGGGACAGAG CGCGTGCGCG GTGTGGCCAG ATACATCTAT
AACCGCGAGG AGTACGGGCG CTTCGACAGC GACGTTGGGG AGTTCCAGGC GGTGACCGAG
CTGGGGCGGA GCATCGAGGA CTGGAACAAC TATAAGGACT TCTTGGAGCA GGAGCGGGCC
GCGGTGGACA AGGTGTGCAG ACACAACTAC GAGGCGGAGC TGCGCACGAC CTTGCAGCGG
CAAGTGGAGC CCACAGTGAC CATCTCCCCA TCCAGGACAG AGGCCCTCAA CCACCACAAC
CTGCTGGTCT GCTCAGTGAC AGATTTCTAT CCAGCCCAGA TCAAAGTCCG GTGGTTTCGG
AATGACCAGG AGGAGACAGC CGGTGTTGTG TCCACCTCCC TCATTAGGAA TGGTGACTGG
ACCTTCCAGA TTCTGGTGAT GCTGGAAATA ACTCCCCAGC GTGGAGACAT CTACACCTGC
CAAGTGGAGC ACCCCAGCCT CCAGAGCCCC ATCACCGTGG AGTGGCGGGC TCAGTCTGAA
TCTGCCCAGA GCAAGATGCT GAGTGGCATT GGAGGCTTCG TGCTGGGGCT GATCTTCCTC
GGGCTGGGCC TTATCATCCG TCACAGGGGT CAGAAAGGAC CTCGAGGGCC TCCACCAGCA
GGTAATATTT CAGCCATGAT CCAGTCAGGG GAGAGGGCAC AGGCATAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005272864.1
CDS63..890
Misc Feature(1)183..395(+)
Misc Feature(2)432..713(+)
Misc Feature(3)447..611(+)
Misc Feature(4)486..704(+)
Misc Feature(5)510..608(+)
Translation
Position Chain Variation Link
3 3 c, t dbSNP:781031809
52 52 c, g, t dbSNP:1573649
53 53 a, g dbSNP:777452739
58 58 a, g dbSNP:79638337
69 69 c, g dbSNP:202238720
71 71 a, g dbSNP:765751400
73 73 a, g dbSNP:759973134
78 78 c, t dbSNP:754341479
80 80 c, t dbSNP:766714258
82 82 c, g dbSNP:761067146
90 90 c, g, t dbSNP:201573322
94 94 c, t dbSNP:200239097
99 99 a, g dbSNP:767726406
102 102 c, g dbSNP:766009346
105 105 a, c dbSNP:572119983
108 108 a, g dbSNP:774505906
118 118 c, t dbSNP:201992526
123 123 c, t dbSNP:768852088
125 125 a, g dbSNP:745856868
128 128 a, c dbSNP:150473207
130 130 c, t dbSNP:770672954
134 134 a, g dbSNP:201129947
145 145 c, g dbSNP:200126619
169 169 c, t dbSNP:764317349
170 170 a, g dbSNP:763095415
171 171 g, t dbSNP:776465671
180 180 a, c dbSNP:770853395
183 183 g, t dbSNP:746793422
204 204 c, g dbSNP:772996134
209 209 a, c dbSNP:771853861
213 213 c, t dbSNP:747859033
216 216 c, g dbSNP:778517822
221 221 c, g, t dbSNP:762815
223 223 c, g dbSNP:780434886
228 228 a, g dbSNP:756573872
237 237 a, t dbSNP:529206424
245 245 c, t dbSNP:768051532
250 250 a, g dbSNP:115793818
257 257 c, t dbSNP:751869503
261 261 c, g dbSNP:764372793
262 262 a, g dbSNP:763148374
266 266 a, c dbSNP:752765802
271 271 g, t dbSNP:766235442
277 277 c, t dbSNP:764659256
278 278 c, t dbSNP:760736981
282 282 a, g dbSNP:773011968
286 286 a, t dbSNP:771906953
302 302 a, g dbSNP:147622494
304 304 c, t dbSNP:557758826
317 317 c, g dbSNP:774012292
334 334 a, g dbSNP:768235138
360 360 g, t dbSNP:748749283
389 389 -, c dbSNP:35282346
393 393 a, c, g dbSNP:201740281
395 395 a, g dbSNP:112964536
396 396 g, t dbSNP:200641501
397 397 c, t dbSNP:199858328
398 398 g, t dbSNP:746434568
399 399 g, t dbSNP:781754459
402 402 c, t dbSNP:77574565
404 404 a, c, g dbSNP:2071551
409 409 a, c dbSNP:751926608
410 410 g, t dbSNP:778155298
421 421 a, g dbSNP:762755845
422 422 a, g dbSNP:752861484
424 424 a, g dbSNP:200476184
433 433 c, t dbSNP:781770566
437 437 a, g dbSNP:771509689
438 438 a, g dbSNP:747382750
439 439 c, t dbSNP:778353470
451 451 a, c dbSNP:758680827
452 452 a, g dbSNP:753015252
461 461 a, g dbSNP:778974230
466 466 c, t dbSNP:755104751
473 473 c, t dbSNP:753854047
475 475 a, t dbSNP:541492094
477 477 a, c, t dbSNP:184113387
478 478 a, g dbSNP:763998181
482 482 c, t dbSNP:762622552
486 486 c, g dbSNP:775238559
489 489 a, g dbSNP:764749284
493 493 a, g dbSNP:759100922
497 497 a, g dbSNP:17850863
501 501 a, c dbSNP:770561591
511 511 a, g dbSNP:559050421
516 516 a, g dbSNP:747580825
517 517 c, g dbSNP:34554556
517 517 c, g dbSNP:773837565
522 522 a, g dbSNP:772529058
532 532 a, g dbSNP:1049110
540 540 a, c, t dbSNP:540615740
541 541 a, c, g dbSNP:576438613
542 542 g, t dbSNP:756164019
547 547 a, g dbSNP:56536369
548 548 c, t dbSNP:763896886
552 552 a, g dbSNP:758233735
561 561 a, g dbSNP:200716952
563 563 c, t dbSNP:200147031
564 564 a, g dbSNP:759329639
566 566 c, t dbSNP:201563919
567 567 a, g, t dbSNP:760233707
569 569 g, t dbSNP:773890444
575 575 c, t dbSNP:772397642
576 576 a, c dbSNP:748548031
577 577 c, t dbSNP:774640477
579 579 c, t dbSNP:769047510
582 582 c, t dbSNP:114145988
584 584 c, t dbSNP:780217889
590 590 a, g dbSNP:756215229
593 593 c, t dbSNP:745898010
597 597 a, g dbSNP:777781697
605 605 c, t dbSNP:758204528
611 611 g, t dbSNP:752675986
612 612 a, g dbSNP:778632746
613 613 g, t dbSNP:754800847
614 614 c, t dbSNP:753587946
618 618 a, g dbSNP:755542673
630 630 a, g dbSNP:766093798
632 632 a, g dbSNP:760288604
641 641 g, t dbSNP:749832708
642 642 c, t dbSNP:115645516
643 643 a, g dbSNP:762281871
647 647 a, c dbSNP:774897099
650 650 c, t dbSNP:769047010
651 651 a, g dbSNP:763261464
658 658 c, t dbSNP:775710959
659 659 c, t dbSNP:769944283
665 665 a, c dbSNP:745952197
669 669 c, g dbSNP:781195954
673 673 a, g dbSNP:771031206
678 678 a, g dbSNP:202167169
681 681 a, c, t dbSNP:754855896
688 688 a, g dbSNP:200827098
695 695 a, c dbSNP:779853574
697 697 c, t dbSNP:199585912
698 698 c, t dbSNP:750074923
699 699 a, g dbSNP:766982756
701 701 a, g dbSNP:761353450
705 705 a, t dbSNP:752035840
707 707 a, g dbSNP:764535884
708 708 c, t dbSNP:763320194
711 711 -, g dbSNP:776142583
716 716 a, g dbSNP:144961216
732 732 a, c dbSNP:556579358
733 733 a, g dbSNP:745625203
734 734 c, g dbSNP:780721175
736 736 a, t dbSNP:756866945
740 740 g, t dbSNP:746637959
741 741 c, g dbSNP:777323311
744 744 a, g dbSNP:9276572
749 749 ca, tg dbSNP:35354072
749 749 c, t dbSNP:9276571
750 750 a, g dbSNP:9276570
753 753 g, t dbSNP:755322600
762 762 a, g dbSNP:754178133
768 768 c, g dbSNP:766518048
770 770 a, g, t dbSNP:773541315
773 773 c, g dbSNP:34988824
782 782 c, t dbSNP:761941061
783 783 a, g, t dbSNP:769713525
788 788 a, g dbSNP:745655214
795 795 a, c dbSNP:776555504
797 797 c, t dbSNP:112126661
801 801 c, t dbSNP:746713265
802 802 a, g dbSNP:77504727
805 805 a, g dbSNP:771604184
806 806 a, c dbSNP:747612237
809 809 g, t dbSNP:779303788
810 810 a, g dbSNP:113761247
812 812 c, t dbSNP:754283114
815 815 g, t dbSNP:780512948
816 816 a, t dbSNP:756400541
823 823 c, g dbSNP:762911265
825 825 c, t dbSNP:566065650
839 839 a, t dbSNP:3213484
848 848 c, t dbSNP:760479732
866 866 g, t dbSNP:772927042
903 903 a, g dbSNP:771853713
912 912 c, g dbSNP:757479315
913 913 c, g, t dbSNP:773840220
914 914 a, g dbSNP:185581808
915 915 -, c dbSNP:775891466

Target ORF information:

RefSeq Version XM_005272807
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005272807

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCTCTGC AGATCCCTGG AGGCTTTTGG GCAGCAGCTG TGACCGTGAT GCTGGTGATG 
CTGAGCACCC CAGTGGCTGA GGCCAGAGAC TTTCCCAAGG ATTTCTTGGT CCAGTTTAAG
GGCATGTGCT ACTTCACCAA CGGGACAGAG CGCGTGCGCG GTGTGGCCAG ATACATCTAT
AACCGCGAGG AGTACGGGCG CTTCGACAGC GACGTTGGGG AGTTCCAGGC GGTGACCGAG
CTGGGGCGGA GCATCGAGGA CTGGAACAAC TATAAGGACT TCTTGGAGCA GGAGCGGGCC
GCGGTGGACA AGGTGTGCAG ACACAACTAC GAGGCGGAGC TGCGCACGAC CTTGCAGCGG
CAAGTGGAGC CCACAGTGAC CATCTCCCCA TCCAGGACAG AGGCCCTCAA CCACCACAAC
CTGCTGGTCT GCTCAGTGAC AGATTTCTAT CCAGCCCAGA TCAAAGTCCG GTGGTTTCGG
AATGACCAGG AGGAGACAGC CGGTGTTGTG TCCACCTCCC TCATTAGGAA TGGTGACTGG
ACCTTCCAGA TTCTGGTGAT GCTGGAAATA ACTCCCCAGC GTGGAGACAT CTACACCTGC
CAAGTGGAGC ACCCCAGCCT CCAGAGCCCC ATCACCGTGG AGTGGCGGGC TCAGTCTGAA
TCTGCCCAGA GCAAGATGCT GAGTGGCATT GGAGGCTTCG TGCTGGGGCT GATCTTCCTC
GGGCTGGGCC TTATCATCCG TCACAGGGGT CAGAAAGGAC CTCGAGGGCC TCCACCAGCA
GGTAATATTT CAGCCATGAT CCAGTCAGGG GAGAGGGCAC AGGCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58240
Accession Version XM_011547242.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 771bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product HLA class II histocompatibility antigen, DQ beta 2 chain isoform X14
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_113891.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCTCTGC AGATCCCTGG AGGCTTTTGG GCAGCAGCTG TGACCGTGAT GCTGGTGATG 
CTGAGCACCC CAGTGGCTGA GGCCAGAGAC TTTCCCAAGG ATTTCTTGGT CCAGTTTAAG
GGCATGTGCT ACTTCACCAA CGGGACAGAG CGCGTGCGCG GTGTGGCCAG ATACATCTAT
AACCGCGAGG AGTACGGGCG CTTCGACAGC GACGTTGGGG AGTTCCAGGC GGTGACCGAG
CTGGGGCGGA GCATCGAGGA CTGGAACAAC TATAAGGACT TCTTGGAGCA GGAGCGGGCC
GCGGTGGACA AGGTGTGCAG ACACAACTAC GAGGCGGAGC TGCGCACGAC CTTGCAGCGG
CAAGTGGAGC CCACAGTGAC CATCTCCCCA TCCAGGACAG AGGCCCTCAA CCACCACAAC
CTGCTGGTCT GCTCAGTGAC AGATTTCTAT CCAGCCCAGA TCAAAGTCCG GTGGTTTCGG
AATGACCAGG AGGAGACAGC CGGTGTTGTG TCCACCTCCC TCATTAGGAA TGGTGACTGG
ACCTTCCAGA TTCTGGTGAT GCTGGAAATA ACTCCCCAGC GTGGAGACAT CTACACCTGC
CAAGTGGAGC ACCCCAGCCT CCAGAGCCCC ATCACCGTGG AGTGGCGGGC TCAGTCTGAA
TCTGCCCAGA GCAAGATGCT GAGTGGCATT GGAGGCTTCG TGCTGGGGCT GATCTTCCTC
GGGCTGGGCC TTATCATCCG TCACAGGGGT CAGAAAGGAC TCCTGCACTG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011545544.1
CDS63..833
Misc Feature(1)183..395(+)
Misc Feature(2)432..713(+)
Misc Feature(3)447..611(+)
Misc Feature(4)486..704(+)
Misc Feature(5)510..608(+)
Translation
Position Chain Variation Link
3 3 c, t dbSNP:781031809
52 52 c, g, t dbSNP:1573649
53 53 a, g dbSNP:777452739
58 58 a, g dbSNP:79638337
69 69 c, g dbSNP:202238720
71 71 a, g dbSNP:765751400
73 73 a, g dbSNP:759973134
78 78 c, t dbSNP:754341479
80 80 c, t dbSNP:766714258
82 82 c, g dbSNP:761067146
90 90 c, g, t dbSNP:201573322
94 94 c, t dbSNP:200239097
99 99 a, g dbSNP:767726406
102 102 c, g dbSNP:766009346
105 105 a, c dbSNP:572119983
108 108 a, g dbSNP:774505906
118 118 c, t dbSNP:201992526
123 123 c, t dbSNP:768852088
125 125 a, g dbSNP:745856868
128 128 a, c dbSNP:150473207
130 130 c, t dbSNP:770672954
134 134 a, g dbSNP:201129947
145 145 c, g dbSNP:200126619
169 169 c, t dbSNP:764317349
170 170 a, g dbSNP:763095415
171 171 g, t dbSNP:776465671
180 180 a, c dbSNP:770853395
183 183 g, t dbSNP:746793422
204 204 c, g dbSNP:772996134
209 209 a, c dbSNP:771853861
213 213 c, t dbSNP:747859033
216 216 c, g dbSNP:778517822
221 221 c, g, t dbSNP:762815
223 223 c, g dbSNP:780434886
228 228 a, g dbSNP:756573872
237 237 a, t dbSNP:529206424
245 245 c, t dbSNP:768051532
250 250 a, g dbSNP:115793818
257 257 c, t dbSNP:751869503
261 261 c, g dbSNP:764372793
262 262 a, g dbSNP:763148374
266 266 a, c dbSNP:752765802
271 271 g, t dbSNP:766235442
277 277 c, t dbSNP:764659256
278 278 c, t dbSNP:760736981
282 282 a, g dbSNP:773011968
286 286 a, t dbSNP:771906953
302 302 a, g dbSNP:147622494
304 304 c, t dbSNP:557758826
317 317 c, g dbSNP:774012292
334 334 a, g dbSNP:768235138
360 360 g, t dbSNP:748749283
389 389 -, c dbSNP:35282346
393 393 a, c, g dbSNP:201740281
395 395 a, g dbSNP:112964536
396 396 g, t dbSNP:200641501
397 397 c, t dbSNP:199858328
398 398 g, t dbSNP:746434568
399 399 g, t dbSNP:781754459
402 402 c, t dbSNP:77574565
404 404 a, c, g dbSNP:2071551
409 409 a, c dbSNP:751926608
410 410 g, t dbSNP:778155298
421 421 a, g dbSNP:762755845
422 422 a, g dbSNP:752861484
424 424 a, g dbSNP:200476184
433 433 c, t dbSNP:781770566
437 437 a, g dbSNP:771509689
438 438 a, g dbSNP:747382750
439 439 c, t dbSNP:778353470
451 451 a, c dbSNP:758680827
452 452 a, g dbSNP:753015252
461 461 a, g dbSNP:778974230
466 466 c, t dbSNP:755104751
473 473 c, t dbSNP:753854047
475 475 a, t dbSNP:541492094
477 477 a, c, t dbSNP:184113387
478 478 a, g dbSNP:763998181
482 482 c, t dbSNP:762622552
486 486 c, g dbSNP:775238559
489 489 a, g dbSNP:764749284
493 493 a, g dbSNP:759100922
497 497 a, g dbSNP:17850863
501 501 a, c dbSNP:770561591
511 511 a, g dbSNP:559050421
516 516 a, g dbSNP:747580825
517 517 c, g dbSNP:34554556
517 517 c, g dbSNP:773837565
522 522 a, g dbSNP:772529058
532 532 a, g dbSNP:1049110
540 540 a, c, t dbSNP:540615740
541 541 a, c, g dbSNP:576438613
542 542 g, t dbSNP:756164019
547 547 a, g dbSNP:56536369
548 548 c, t dbSNP:763896886
552 552 a, g dbSNP:758233735
561 561 a, g dbSNP:200716952
563 563 c, t dbSNP:200147031
564 564 a, g dbSNP:759329639
566 566 c, t dbSNP:201563919
567 567 a, g, t dbSNP:760233707
569 569 g, t dbSNP:773890444
575 575 c, t dbSNP:772397642
576 576 a, c dbSNP:748548031
577 577 c, t dbSNP:774640477
579 579 c, t dbSNP:769047510
582 582 c, t dbSNP:114145988
584 584 c, t dbSNP:780217889
590 590 a, g dbSNP:756215229
593 593 c, t dbSNP:745898010
597 597 a, g dbSNP:777781697
605 605 c, t dbSNP:758204528
611 611 g, t dbSNP:752675986
612 612 a, g dbSNP:778632746
613 613 g, t dbSNP:754800847
614 614 c, t dbSNP:753587946
618 618 a, g dbSNP:755542673
630 630 a, g dbSNP:766093798
632 632 a, g dbSNP:760288604
641 641 g, t dbSNP:749832708
642 642 c, t dbSNP:115645516
643 643 a, g dbSNP:762281871
647 647 a, c dbSNP:774897099
650 650 c, t dbSNP:769047010
651 651 a, g dbSNP:763261464
658 658 c, t dbSNP:775710959
659 659 c, t dbSNP:769944283
665 665 a, c dbSNP:745952197
669 669 c, g dbSNP:781195954
673 673 a, g dbSNP:771031206
678 678 a, g dbSNP:202167169
681 681 a, c, t dbSNP:754855896
688 688 a, g dbSNP:200827098
695 695 a, c dbSNP:779853574
697 697 c, t dbSNP:199585912
698 698 c, t dbSNP:750074923
699 699 a, g dbSNP:766982756
701 701 a, g dbSNP:761353450
705 705 a, t dbSNP:752035840
707 707 a, g dbSNP:764535884
708 708 c, t dbSNP:763320194
711 711 -, g dbSNP:776142583
716 716 a, g dbSNP:144961216
732 732 a, c dbSNP:556579358
733 733 a, g dbSNP:745625203
734 734 c, g dbSNP:780721175
736 736 a, t dbSNP:756866945
740 740 g, t dbSNP:746637959
741 741 c, g dbSNP:777323311
744 744 a, g dbSNP:9276572
749 749 ca, tg dbSNP:35354072
749 749 c, t dbSNP:9276571
750 750 a, g dbSNP:9276570
753 753 g, t dbSNP:755322600
762 762 a, g dbSNP:754178133
768 768 c, g dbSNP:766518048
770 770 a, g, t dbSNP:773541315
773 773 c, g dbSNP:34988824
782 782 c, t dbSNP:761941061
783 783 a, g, t dbSNP:769713525
788 788 a, g dbSNP:745655214
795 795 a, c dbSNP:776555504
797 797 c, t dbSNP:112126661
801 801 c, t dbSNP:746713265
802 802 a, g dbSNP:77504727
805 805 a, g dbSNP:771604184
806 806 a, c dbSNP:747612237
809 809 g, t dbSNP:779303788
810 810 a, g dbSNP:113761247
812 812 c, t dbSNP:754283114
815 815 g, t dbSNP:780512948
816 816 a, t dbSNP:756400541
822 822 -, c dbSNP:765272140
839 839 a, g dbSNP:756114985
849 849 c, g dbSNP:750351833
874 874 g, t dbSNP:7774954
883 883 a, c dbSNP:761552899
884 884 c, t dbSNP:746579816
908 908 c, t dbSNP:561090370
916 916 g, t dbSNP:7774929
920 920 c, t dbSNP:6457646
927 927 c, g dbSNP:746156224
950 950 c, t dbSNP:190811600
965 965 -, ca dbSNP:777356205
988 988 c, t dbSNP:7774644
1002 1002 c, t dbSNP:9276558
1003 1003 a, g dbSNP:556703494
1005 1005 c, t dbSNP:145591506
1020 1020 c, t dbSNP:3656
1076 1076 a, g dbSNP:372075576
1080 1080 a, g dbSNP:149712668
1135 1135 a, g dbSNP:549347684
1146 1146 a, g dbSNP:7756516
1177 1177 a, g dbSNP:186400259

Target ORF information:

RefSeq Version XM_011547242
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011547242

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCTCTGC AGATCCCTGG AGGCTTTTGG GCAGCAGCTG TGACCGTGAT GCTGGTGATG 
CTGAGCACCC CAGTGGCTGA GGCCAGAGAC TTTCCCAAGG ATTTCTTGGT CCAGTTTAAG
GGCATGTGCT ACTTCACCAA CGGGACAGAG CGCGTGCGCG GTGTGGCCAG ATACATCTAT
AACCGCGAGG AGTACGGGCG CTTCGACAGC GACGTTGGGG AGTTCCAGGC GGTGACCGAG
CTGGGGCGGA GCATCGAGGA CTGGAACAAC TATAAGGACT TCTTGGAGCA GGAGCGGGCC
GCGGTGGACA AGGTGTGCAG ACACAACTAC GAGGCGGAGC TGCGCACGAC CTTGCAGCGG
CAAGTGGAGC CCACAGTGAC CATCTCCCCA TCCAGGACAG AGGCCCTCAA CCACCACAAC
CTGCTGGTCT GCTCAGTGAC AGATTTCTAT CCAGCCCAGA TCAAAGTCCG GTGGTTTCGG
AATGACCAGG AGGAGACAGC CGGTGTTGTG TCCACCTCCC TCATTAGGAA TGGTGACTGG
ACCTTCCAGA TTCTGGTGAT GCTGGAAATA ACTCCCCAGC GTGGAGACAT CTACACCTGC
CAAGTGGAGC ACCCCAGCCT CCAGAGCCCC ATCACCGTGG AGTGGCGGGC TCAGTCTGAA
TCTGCCCAGA GCAAGATGCT GAGTGGCATT GGAGGCTTCG TGCTGGGGCT GATCTTCCTC
GGGCTGGGCC TTATCATCCG TCACAGGGGT CAGAAAGGAC TCCTGCACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58241
Accession Version XM_011547243.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 660bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product HLA class II histocompatibility antigen, DQ beta 2 chain isoform X15
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_113891.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGGCTCTGC AGATCCCTGG AGGCTTTTGG GCAGCAGCTG TGACCGTGAT GCTGGTGATG 
CTGAGCACCC CAGTGGCTGA GGCCAGAGAC TTTCCCAAGG ATTTCTTGGT CCAGTTTAAG
GGCATGTGCT ACTTCACCAA CGGGACAGAG CGCGTGCGCG GTGTGGCCAG ATACATCTAT
AACCGCGAGG AGTACGGGCG CTTCGACAGC GACGTTGGGG AGTTCCAGGC GGTGACCGAG
CTGGGGCGGA GCATCGAGGA CTGGAACAAC TATAAGGACT TCTTGGAGCA GGAGCGGGCC
GCGGTGGACA AGGTGTGCAG ACACAACTAC GAGGCGGAGC TGCGCACGAC CTTGCAGCGG
CAAGTGGAGC CCACAGTGAC CATCTCCCCA TCCAGGACAG AGGCCCTCAA CCACCACAAC
CTGCTGGTCT GCTCAGTGAC AGATTTCTAT CCAGCCCAGA TCAAAGTCCG GTGGTTTCGG
AATGACCAGG AGGAGACAGC CGGTGTTGTG TCCACCTCCC TCATTAGGAA TGGTGACTGG
ACCTTCCAGA TTCTGGTGAT GCTGGAAATA ACTCCCCAGC GTGGAGACAT CTACACCTGC
CAAGTGGAGC ACCCCAGCCT CCAGAGCCCC ATCACCGTGG AGTGGCGACT CCTGCACTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011545545.1
CDS63..722
Misc Feature(1)183..395(+)
Misc Feature(2)432..710(+)
Misc Feature(3)447..611(+)
Misc Feature(4)486..704(+)
Misc Feature(5)510..608(+)
Translation
Position Chain Variation Link
3 3 c, t dbSNP:781031809
52 52 c, g, t dbSNP:1573649
53 53 a, g dbSNP:777452739
58 58 a, g dbSNP:79638337
69 69 c, g dbSNP:202238720
71 71 a, g dbSNP:765751400
73 73 a, g dbSNP:759973134
78 78 c, t dbSNP:754341479
80 80 c, t dbSNP:766714258
82 82 c, g dbSNP:761067146
90 90 c, g, t dbSNP:201573322
94 94 c, t dbSNP:200239097
99 99 a, g dbSNP:767726406
102 102 c, g dbSNP:766009346
105 105 a, c dbSNP:572119983
108 108 a, g dbSNP:774505906
118 118 c, t dbSNP:201992526
123 123 c, t dbSNP:768852088
125 125 a, g dbSNP:745856868
128 128 a, c dbSNP:150473207
130 130 c, t dbSNP:770672954
134 134 a, g dbSNP:201129947
145 145 c, g dbSNP:200126619
169 169 c, t dbSNP:764317349
170 170 a, g dbSNP:763095415
171 171 g, t dbSNP:776465671
180 180 a, c dbSNP:770853395
183 183 g, t dbSNP:746793422
204 204 c, g dbSNP:772996134
209 209 a, c dbSNP:771853861
213 213 c, t dbSNP:747859033
216 216 c, g dbSNP:778517822
221 221 c, g, t dbSNP:762815
223 223 c, g dbSNP:780434886
228 228 a, g dbSNP:756573872
237 237 a, t dbSNP:529206424
245 245 c, t dbSNP:768051532
250 250 a, g dbSNP:115793818
257 257 c, t dbSNP:751869503
261 261 c, g dbSNP:764372793
262 262 a, g dbSNP:763148374
266 266 a, c dbSNP:752765802
271 271 g, t dbSNP:766235442
277 277 c, t dbSNP:764659256
278 278 c, t dbSNP:760736981
282 282 a, g dbSNP:773011968
286 286 a, t dbSNP:771906953
302 302 a, g dbSNP:147622494
304 304 c, t dbSNP:557758826
317 317 c, g dbSNP:774012292
334 334 a, g dbSNP:768235138
360 360 g, t dbSNP:748749283
389 389 -, c dbSNP:35282346
393 393 a, c, g dbSNP:201740281
395 395 a, g dbSNP:112964536
396 396 g, t dbSNP:200641501
397 397 c, t dbSNP:199858328
398 398 g, t dbSNP:746434568
399 399 g, t dbSNP:781754459
402 402 c, t dbSNP:77574565
404 404 a, c, g dbSNP:2071551
409 409 a, c dbSNP:751926608
410 410 g, t dbSNP:778155298
421 421 a, g dbSNP:762755845
422 422 a, g dbSNP:752861484
424 424 a, g dbSNP:200476184
433 433 c, t dbSNP:781770566
437 437 a, g dbSNP:771509689
438 438 a, g dbSNP:747382750
439 439 c, t dbSNP:778353470
451 451 a, c dbSNP:758680827
452 452 a, g dbSNP:753015252
461 461 a, g dbSNP:778974230
466 466 c, t dbSNP:755104751
473 473 c, t dbSNP:753854047
475 475 a, t dbSNP:541492094
477 477 a, c, t dbSNP:184113387
478 478 a, g dbSNP:763998181
482 482 c, t dbSNP:762622552
486 486 c, g dbSNP:775238559
489 489 a, g dbSNP:764749284
493 493 a, g dbSNP:759100922
497 497 a, g dbSNP:17850863
501 501 a, c dbSNP:770561591
511 511 a, g dbSNP:559050421
516 516 a, g dbSNP:747580825
517 517 c, g dbSNP:34554556
517 517 c, g dbSNP:773837565
522 522 a, g dbSNP:772529058
532 532 a, g dbSNP:1049110
540 540 a, c, t dbSNP:540615740
541 541 a, c, g dbSNP:576438613
542 542 g, t dbSNP:756164019
547 547 a, g dbSNP:56536369
548 548 c, t dbSNP:763896886
552 552 a, g dbSNP:758233735
561 561 a, g dbSNP:200716952
563 563 c, t dbSNP:200147031
564 564 a, g dbSNP:759329639
566 566 c, t dbSNP:201563919
567 567 a, g, t dbSNP:760233707
569 569 g, t dbSNP:773890444
575 575 c, t dbSNP:772397642
576 576 a, c dbSNP:748548031
577 577 c, t dbSNP:774640477
579 579 c, t dbSNP:769047510
582 582 c, t dbSNP:114145988
584 584 c, t dbSNP:780217889
590 590 a, g dbSNP:756215229
593 593 c, t dbSNP:745898010
597 597 a, g dbSNP:777781697
605 605 c, t dbSNP:758204528
611 611 g, t dbSNP:752675986
612 612 a, g dbSNP:778632746
613 613 g, t dbSNP:754800847
614 614 c, t dbSNP:753587946
618 618 a, g dbSNP:755542673
630 630 a, g dbSNP:766093798
632 632 a, g dbSNP:760288604
641 641 g, t dbSNP:749832708
642 642 c, t dbSNP:115645516
643 643 a, g dbSNP:762281871
647 647 a, c dbSNP:774897099
650 650 c, t dbSNP:769047010
651 651 a, g dbSNP:763261464
658 658 c, t dbSNP:775710959
659 659 c, t dbSNP:769944283
665 665 a, c dbSNP:745952197
669 669 c, g dbSNP:781195954
673 673 a, g dbSNP:771031206
678 678 a, g dbSNP:202167169
681 681 a, c, t dbSNP:754855896
688 688 a, g dbSNP:200827098
695 695 a, c dbSNP:779853574
697 697 c, t dbSNP:199585912
698 698 c, t dbSNP:750074923
699 699 a, g dbSNP:766982756
701 701 a, g dbSNP:761353450
705 705 a, t dbSNP:752035840
707 707 a, g dbSNP:764535884
708 708 c, t dbSNP:763320194
711 711 -, c dbSNP:765272140
728 728 a, g dbSNP:756114985
738 738 c, g dbSNP:750351833
763 763 g, t dbSNP:7774954
772 772 a, c dbSNP:761552899
773 773 c, t dbSNP:746579816
797 797 c, t dbSNP:561090370
805 805 g, t dbSNP:7774929
809 809 c, t dbSNP:6457646
816 816 c, g dbSNP:746156224
839 839 c, t dbSNP:190811600
854 854 -, ca dbSNP:777356205
877 877 c, t dbSNP:7774644
891 891 c, t dbSNP:9276558
892 892 a, g dbSNP:556703494
894 894 c, t dbSNP:145591506
909 909 c, t dbSNP:3656
965 965 a, g dbSNP:372075576
969 969 a, g dbSNP:149712668
1024 1024 a, g dbSNP:549347684
1035 1035 a, g dbSNP:7756516
1066 1066 a, g dbSNP:186400259

Target ORF information:

RefSeq Version XM_011547243
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens major histocompatibility complex, class II, DQ beta 2 (HLA-DQB2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011547243

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCTCTGC AGATCCCTGG AGGCTTTTGG GCAGCAGCTG TGACCGTGAT GCTGGTGATG 
CTGAGCACCC CAGTGGCTGA GGCCAGAGAC TTTCCCAAGG ATTTCTTGGT CCAGTTTAAG
GGCATGTGCT ACTTCACCAA CGGGACAGAG CGCGTGCGCG GTGTGGCCAG ATACATCTAT
AACCGCGAGG AGTACGGGCG CTTCGACAGC GACGTTGGGG AGTTCCAGGC GGTGACCGAG
CTGGGGCGGA GCATCGAGGA CTGGAACAAC TATAAGGACT TCTTGGAGCA GGAGCGGGCC
GCGGTGGACA AGGTGTGCAG ACACAACTAC GAGGCGGAGC TGCGCACGAC CTTGCAGCGG
CAAGTGGAGC CCACAGTGAC CATCTCCCCA TCCAGGACAG AGGCCCTCAA CCACCACAAC
CTGCTGGTCT GCTCAGTGAC AGATTTCTAT CCAGCCCAGA TCAAAGTCCG GTGGTTTCGG
AATGACCAGG AGGAGACAGC CGGTGTTGTG TCCACCTCCC TCATTAGGAA TGGTGACTGG
ACCTTCCAGA TTCTGGTGAT GCTGGAAATA ACTCCCCAGC GTGGAGACAT CTACACCTGC
CAAGTGGAGC ACCCCAGCCT CCAGAGCCCC ATCACCGTGG AGTGGCGACT CCTGCACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58242
Accession Version XM_011547646.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 783bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product HLA class II histocompatibility antigen, DQ beta 2 chain isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167245.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTCTTGGA AGATGGCTCT GCAGATCCCT GGAGGCTTTT GGGCAGCAGC TGTGACCGTG 
ATGCTGGTGA TGCTGAGCAC CCCAGTGGCT GAGGCCAGAG ACTTTCCCAA GGATTTCTTG
GTCCAGTTTA AGGGCATGTG CTACTTCACC AACGGGACAG AGCGCGTGCG GGGTGTGGCC
AGATACATCT ATAACCGCGA GGAGTACGGG CGCTTCGACA GCGACGTTGG GGAGTTCCAG
GCGGTGACCG AGCTGGGGCG GAGCATCGAG GACTGGAACA ACTATAAGGA CTTCTTGGAG
CAGGAGCGGG CCGCGGTGGA CAAGGTGTGC AGACACAACT ACGAGGCGGA GCTACGCACG
ACCTTGCAGC GGCAAGTGGA GCCCACAGTG ACCATCTCCC CATCCAGGAC AGAGGCCCTC
AACCACCACA ACCTGCTGGT CTGCTCAGTG ACAGATTTCT ATCCAGCCCA GATCAAAGTC
CAGTGGTTTC GGAATGACCA GGAGGAGACA GCCGGTGTTG TGTCCACCTC CCTCATTAGG
AATGGTGACT GGACCTTCCA GATTCTGGTG ATGCTGGAAA TAACTCCCCA GCGTGGAGAC
ATCTACACCT GCCAAGTGGA GCACCCCAGC CTCCAGAGCC CCATCACCGT GGAGTGGCGG
GCTCAGTCTG AATCTGCCCA GAGCAAGATG CTGGGTGGTG TTGGAGGCTT CGTGCTGGGG
CTGATCTTCC TCGGGCTGGG CCTTATCATC CGTCACAGGG GTCAGAAAGG ACTCCTGCAC
TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011545948.1
CDS66..848
Misc Feature(1)198..410(+)
Misc Feature(2)447..728(+)
Misc Feature(3)462..626(+)
Misc Feature(4)501..719(+)
Misc Feature(5)525..623(+)
Translation
Position Chain Variation Link
12 12 a, t dbSNP:9276585
18 18 c, t dbSNP:781031809
67 67 c, g, t dbSNP:1573649
68 68 a, g dbSNP:777452739
73 73 a, g dbSNP:79638337
84 84 c, g dbSNP:202238720
86 86 a, g dbSNP:765751400
88 88 a, g dbSNP:759973134
93 93 c, t dbSNP:754341479
95 95 c, t dbSNP:766714258
97 97 c, g dbSNP:761067146
105 105 c, g, t dbSNP:201573322
109 109 c, t dbSNP:200239097
114 114 a, g dbSNP:767726406
117 117 c, g dbSNP:766009346
120 120 a, c dbSNP:572119983
123 123 a, g dbSNP:774505906
133 133 c, t dbSNP:201992526
138 138 c, t dbSNP:768852088
140 140 a, g dbSNP:745856868
143 143 a, c dbSNP:150473207
145 145 c, t dbSNP:770672954
149 149 a, g dbSNP:201129947
160 160 c, g dbSNP:200126619
184 184 c, t dbSNP:764317349
185 185 a, g dbSNP:763095415
186 186 g, t dbSNP:776465671
195 195 a, c dbSNP:770853395
198 198 g, t dbSNP:746793422
219 219 c, g dbSNP:772996134
224 224 a, c dbSNP:771853861
228 228 c, t dbSNP:747859033
231 231 c, g dbSNP:778517822
236 236 c, g, t dbSNP:762815
238 238 c, g dbSNP:780434886
243 243 a, g dbSNP:756573872
252 252 a, t dbSNP:529206424
260 260 c, t dbSNP:768051532
265 265 a, g dbSNP:115793818
272 272 c, t dbSNP:751869503
276 276 c, g dbSNP:764372793
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293 293 c, t dbSNP:760736981
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349 349 a, g dbSNP:768235138
375 375 g, t dbSNP:748749283
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414 414 g, t dbSNP:781754459
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594 594 c, t dbSNP:769047510
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889