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HLA-DRB1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol HLA-DRB1
Entrez Gene ID 3123
Full Name major histocompatibility complex, class II, DR beta 1
Synonyms DRB1, DRw10, HLA-DR1B, HLA-DRB, SS1
General protein information
Preferred Names
major histocompatibility complex, class II, DR beta 1
Names
major histocompatibility complex, class II, DR beta 1
DW2.2/DR2.2
MHC class II antigen
lymphocyte antigen DRB1
MHC class II HLA-DRw10-beta
human leucocyte antigen DRB1
MHC class II HLA-DR beta 1 chain
MHC class II HLA-DR-beta cell surface glycoprotein
HLA class II histocompatibility antigen, DR-1 beta chain
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p21.3

Summary HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

142857

Disorder Html: {Pemphigoid, susceptibility to} (2); {Sarcoidosis, susceptibility

mRNA and Protein(s)

mRNA Protein Name
NM_002124 NP_002115 major histocompatibility complex, class II, DR beta 1 precursor
NM_001243965 NP_001230894 major histocompatibility complex, class II, DR beta 1 precursor



Homo sapiens (human) HLA-DRB1 NP_002115.2
Pan troglodytes (chimpanzee) PATR-DQB1 NP_001258000.1
Macaca mulatta (Rhesus monkey) LOC705588 XP_001089877.1
Macaca mulatta (Rhesus monkey) MAMU-DRB5 XP_002803731.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HLA-DRB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HLA-DRB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu27047
NM_002124 Homo sapiens major histocompatibility complex, class II, DR beta 1 (HLA-DRB1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$154.50-$216.30
$309.00
OHu29136
NM_001243965 Homo sapiens major histocompatibility complex, class II, DR beta 1 (HLA-DRB1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu27047
Clone ID Related Accession (Same CDS sequence) NM_002124
Accession Version NM_002124.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 801bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product major histocompatibility complex, class II, DR beta 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK291987.1 and BC033827.1. On or before Feb 15, 2014 this sequence version replaced gi:578841493, gi:578841740, gi:155030210. Transcript Variant: This variant (1) represents the DRB1*15:01:01:01 allele of the HLA-DRB1 gene, as represented in the assembled chromosome 6 in the primary assembly of the reference genome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC033827.1, BC007920.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147920, SAMEA2154405 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGTGTGTC TGAAGCTCCC TGGAGGCTCC TGCATGACAG CGCTGACAGT GACACTGATG 
GTGCTGAGCT CCCCACTGGC TTTGTCTGGG GACACCCGAC CACGTTTCCT GTGGCAGCCT
AAGAGGGAGT GTCATTTCTT CAATGGGACG GAGCGGGTGC GGTTCCTGGA CAGATACTTC
TATAACCAGG AGGAGTCCGT GCGCTTCGAC AGCGACGTGG GGGAGTTCCG GGCGGTGACG
GAGCTGGGGC GGCCTGACGC TGAGTACTGG AACAGCCAGA AGGACATCCT GGAGCAGGCG
CGGGCCGCGG TGGACACCTA CTGCAGACAC AACTACGGGG TTGTGGAGAG CTTCACAGTG
CAGCGGCGAG TCCAACCTAA GGTGACTGTA TATCCTTCAA AGACCCAGCC CCTGCAGCAC
CACAACCTCC TGGTCTGCTC TGTGAGTGGT TTCTATCCAG GCAGCATTGA AGTCAGGTGG
TTCCTGAACG GCCAGGAAGA GAAGGCTGGG ATGGTGTCCA CAGGCCTGAT CCAGAATGGA
GACTGGACCT TCCAGACCCT GGTGATGCTG GAAACAGTTC CTCGAAGTGG AGAGGTTTAC
ACCTGCCAAG TGGAGCACCC AAGCGTGACA AGCCCTCTCA CAGTGGAATG GAGAGCACGG
TCTGAATCTG CACAGAGCAA GATGCTGAGT GGAGTCGGGG GCTTTGTGCT GGGCCTGCTC
TTCCTTGGGG CCGGGCTGTT CATCTACTTC AGGAATCAGA AAGGACACTC TGGACTTCAG
CCAACAGGAT TCCTGAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002115.2
CDS95..895
Misc Feature(1)47..49(+)
Misc Feature(2)182..466(+)
Misc Feature(3)221..442(+)
Misc Feature(4)467..775(+)
Misc Feature(5)470..751(+)
Misc Feature(6)485..649(+)
Misc Feature(7)548..646(+)
Misc Feature(8)776..838(+)
Exon (1)1..194
Gene:HLA-DRB1
Gene Synonym:
Exon (2)195..464
Gene:HLA-DRB1
Gene Synonym:
Exon (3)465..746
Gene:HLA-DRB1
Gene Synonym:
Exon (4)747..857
Gene:HLA-DRB1
Gene Synonym:
Exon (5)858..881
Gene:HLA-DRB1
Gene Synonym:
Exon (6)882..1217
Gene:HLA-DRB1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002124
Organism Homo sapiens (human)
Definition Homo sapiens major histocompatibility complex, class II, DR beta 1 (HLA-DRB1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002124

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGTGTGTC TGAAGCTCCC TGGAGGCTCC TGCATGACAG CGCTGACAGT GACACTGATG 
GTGCTGAGCT CCCCACTGGC TTTGTCTGGG GACACCCGAC CACGTTTCCT GTGGCAGCCT
AAGAGGGAGT GTCATTTCTT CAATGGGACG GAGCGGGTGC GGTTCCTGGA CAGATACTTC
TATAACCAGG AGGAGTCCGT GCGCTTCGAC AGCGACGTGG GGGAGTTCCG GGCGGTGACG
GAGCTGGGGC GGCCTGACGC TGAGTACTGG AACAGCCAGA AGGACATCCT GGAGCAGGCG
CGGGCCGCGG TGGACACCTA CTGCAGACAC AACTACGGGG TTGTGGAGAG CTTCACAGTG
CAGCGGCGAG TCCAACCTAA GGTGACTGTA TATCCTTCAA AGACCCAGCC CCTGCAGCAC
CACAACCTCC TGGTCTGCTC TGTGAGTGGT TTCTATCCAG GCAGCATTGA AGTCAGGTGG
TTCCTGAACG GCCAGGAAGA GAAGGCTGGG ATGGTGTCCA CAGGCCTGAT CCAGAATGGA
GACTGGACCT TCCAGACCCT GGTGATGCTG GAAACAGTTC CTCGAAGTGG AGAGGTTTAC
ACCTGCCAAG TGGAGCACCC AAGCGTGACA AGCCCTCTCA CAGTGGAATG GAGAGCACGG
TCTGAATCTG CACAGAGCAA GATGCTGAGT GGAGTCGGGG GCTTTGTGCT GGGCCTGCTC
TTCCTTGGGG CCGGGCTGTT CATCTACTTC AGGAATCAGA AAGGACACTC TGGACTTCAG
CCAACAGGAT TCCTGAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu29136
Clone ID Related Accession (Same CDS sequence) NM_001243965
Accession Version NM_001243965.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 801bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product major histocompatibility complex, class II, DR beta 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB246210.1, X00699.1 and BM671866.1. This sequence is a reference standard in the RefSeqGene project. On Jul 26, 2012 this sequence version replaced gi:397138357. Transcript Variant: This variant (2) represents the DRB1*03:01:01:01 allele of the HLA-DRB1 gene, as represented in the alternate locus group ALT_REF_LOCI_2 of the reference genome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK291987.1, AK293020.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147920, SAMEA2154405 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGTGTGTC TGAGGCTCCC TGGAGGCTCC TGCATGGCAG TTCTGACAGT GACACTGATG 
GTGCTGAGCT CCCCACTGGC TTTGGCTGGG GACACCAGAC CACGTTTCTT GGAGTACTCT
ACGTCTGAGT GTCATTTCTT CAATGGGACG GAGCGGGTGC GGTACCTGGA CAGATACTTC
CATAACCAGG AGGAGAACGT GCGCTTCGAC AGCGACGTGG GGGAGTTCCG GGCGGTGACG
GAGCTGGGGC GGCCTGATGC CGAGTACTGG AACAGCCAGA AGGACCTCCT GGAGCAGAAG
CGGGGCCGGG TGGACAACTA CTGCAGACAC AACTACGGGG TTGTGGAGAG CTTCACAGTG
CAGCGGCGAG TCCATCCTAA GGTGACTGTG TATCCTTCAA AGACCCAGCC CCTGCAGCAC
CATAACCTCC TGGTCTGTTC TGTGAGTGGT TTCTATCCAG GCAGCATTGA AGTCAGGTGG
TTCCGGAATG GCCAGGAAGA GAAGACTGGG GTGGTGTCCA CAGGCCTGAT CCACAATGGA
GACTGGACCT TCCAGACCCT GGTGATGCTG GAAACAGTTC CTCGGAGTGG AGAGGTTTAC
ACCTGCCAAG TGGAGCACCC AAGCGTGACA AGCCCTCTCA CAGTGGAATG GAGAGCACGG
TCTGAATCTG CACAGAGCAA GATGCTGAGT GGAGTCGGGG GCTTTGTGCT GGGCCTGCTC
TTCCTTGGGG CCGGGCTGTT CATCTACTTC AGGAATCAGA AAGGACACTC TGGACTTCAG
CCAAGAGGAT TCCTGAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001230894.1
CDS95..895
Misc Feature(1)47..49(+)
Misc Feature(2)182..463(+)
Misc Feature(3)218..442(+)
Misc Feature(4)464..745(+)
Misc Feature(5)470..751(+)
Misc Feature(6)746..775(+)
Misc Feature(7)776..844(+)
Exon (1)1..194
Gene:HLA-DRB1
Gene Synonym:
Exon (2)195..464
Gene:HLA-DRB1
Gene Synonym:
Exon (3)465..746
Gene:HLA-DRB1
Gene Synonym:
Exon (4)747..857
Gene:HLA-DRB1
Gene Synonym:
Exon (5)858..881
Gene:HLA-DRB1
Gene Synonym:
Exon (6)882..1218
Gene:HLA-DRB1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001243965
Organism Homo sapiens (human)
Definition Homo sapiens major histocompatibility complex, class II, DR beta 1 (HLA-DRB1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001243965

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGTGTGTC TGAGGCTCCC TGGAGGCTCC TGCATGGCAG TTCTGACAGT GACACTGATG 
GTGCTGAGCT CCCCACTGGC TTTGGCTGGG GACACCAGAC CACGTTTCTT GGAGTACTCT
ACGTCTGAGT GTCATTTCTT CAATGGGACG GAGCGGGTGC GGTACCTGGA CAGATACTTC
CATAACCAGG AGGAGAACGT GCGCTTCGAC AGCGACGTGG GGGAGTTCCG GGCGGTGACG
GAGCTGGGGC GGCCTGATGC CGAGTACTGG AACAGCCAGA AGGACCTCCT GGAGCAGAAG
CGGGGCCGGG TGGACAACTA CTGCAGACAC AACTACGGGG TTGTGGAGAG CTTCACAGTG
CAGCGGCGAG TCCATCCTAA GGTGACTGTG TATCCTTCAA AGACCCAGCC CCTGCAGCAC
CATAACCTCC TGGTCTGTTC TGTGAGTGGT TTCTATCCAG GCAGCATTGA AGTCAGGTGG
TTCCGGAATG GCCAGGAAGA GAAGACTGGG GTGGTGTCCA CAGGCCTGAT CCACAATGGA
GACTGGACCT TCCAGACCCT GGTGATGCTG GAAACAGTTC CTCGGAGTGG AGAGGTTTAC
ACCTGCCAAG TGGAGCACCC AAGCGTGACA AGCCCTCTCA CAGTGGAATG GAGAGCACGG
TCTGAATCTG CACAGAGCAA GATGCTGAGT GGAGTCGGGG GCTTTGTGCT GGGCCTGCTC
TTCCTTGGGG CCGGGCTGTT CATCTACTTC AGGAATCAGA AAGGACACTC TGGACTTCAG
CCAAGAGGAT TCCTGAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Interaction between adolescent obesity and HLA risk genes in the etiology of multiple sclerosis
Neurology 82 (10), 865-872 (2014)
Hedstrom AK, Lima Bomfim I, Barcellos L, Gianfrancesco M, Schaefer C, Kockum I, Olsson T and Alfredsson L.


book

HLA-DR, DQ class II DNA typing in pediatric panuveitis and tubulointerstitial nephritis and uveitis
Am. J. Ophthalmol. 157 (3), 678-686 (2014)
Reddy AK, Hwang YS, Mandelcorn ED and Davis JL.


book

Novel rheumatoid arthritis susceptibility locus at 22q12 identified in an extended UK genome-wide association study
Arthritis Rheumatol 66 (1), 24-30 (2014)
Orozco G, Viatte S, Bowes J, Martin P, Wilson AG, Morgan AW, Steer S, Wordsworth P, Hocking LJ, Barton A, Worthington J and Eyre S.


book

HLA-DRB1 associations with rheumatoid arthritis-related pulmonary fibrosis
Scand. J. Rheumatol. 43 (1), 75-76 (2014)
Ennis H, Gupta A, Dawson J, Lunt M, Thomson W and Herrick A.


book

A molecular basis for the association of the HLA-DRB1 locus, citrullination, and rheumatoid arthritis
J. Exp. Med. 210 (12), 2569-2582 (2013)
Scally SW, Petersen J, Law SC, Dudek NL, Nel HJ, Loh KL, Wijeyewickrema LC, Eckle SB, van Heemst J, Pike RN, McCluskey J, Toes RE, La Gruta NL, Purcell AW, Reid HH, Thomas R and Rossjohn J.


book

HLA-DRB1 first domain sequence for a novel DR4 allele designated DRB1*0413
Tissue Antigens 40 (5), 267-268 (1992)
Petersdorf EW, Smith AG, Martin PJ and Hansen JA.


book

Molecular analysis of HLA-DRB1*08/12 alleles: identification of two additional alleles
Hum. Immunol. 34 (1), 24-30 (1992)
Eberle M and Baxter-Lowe LA.


book

Molecular genetic analysis of HLA-DR and HLA-DQ genes among anti-U1-70-kd autoantibody positive connective tissue disease patients
Arthritis Rheum. 35 (1), 83-94 (1992)
Kaneoka H, Hsu KC, Takeda Y, Sharp GC and Hoffman RW.


book

The evolutionary origin of the HLA-DR3 haplotype
Immunogenetics 35 (4), 263-271 (1992)
Vincek V, Klein D, Figueroa F, Hauptfeld V, Kasahara M, O'hUigin C, Mach B and Klein J.


book

Mhc-DRB diversity of the chimpanzee (Pan troglodytes)
Immunogenetics 37 (1), 1-11 (1992)
Kenter M, Otting N, Anholts J, Jonker M, Schipper R and Bontrop RE.


 
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