×

HPGD cDNA ORF clone, Homo sapiens (human)

Gene Symbol HPGD
Entrez Gene ID 3248
Full Name hydroxyprostaglandin dehydrogenase 15-(NAD)
Synonyms 15-PGDH, PGDH, PGDH1, PHOAR1, SDR36C1
General protein information
Preferred Names
15-hydroxyprostaglandin dehydrogenase [NAD(+)]
Names
15-hydroxyprostaglandin dehydrogenase [NAD(+)]
prostaglandin dehydrogenase 1
NAD+-dependent 15-hydroxyprostaglandin dehydrogenase
short chain dehydrogenase/reductase family 36C, member 1
NP_000851.2
NP_001139288.1
NP_001243230.1
NP_001243234.1
NP_001243235.1
NP_001243236.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

4

4q34-q35

Summary This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]. lac of sum
Disorder MIM:

601688

Disorder Html: Cranioosteoarthropathy, 259100 (3); Hypertrophic osteoarthropathy,

mRNA and Protein(s)

mRNA Protein Name
XM_011531907 XP_011530209 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform X1
NM_001256306 NP_001243235 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 5
NM_001256301 NP_001243230 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 3
NM_001145816 NP_001139288 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 2
NM_001256307 NP_001243236 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 3
NM_001256305 NP_001243234 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 4
NM_000860 NP_000851 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 1



Homo sapiens (human) HPGD NP_000851.2
Pan troglodytes (chimpanzee) HPGD XP_517542.2
Macaca mulatta (Rhesus monkey) HPGD XP_001087957.1
Canis lupus familiaris (dog) HPGD XP_543199.1
Bos taurus (cattle) HPGD NP_001029591.1
Mus musculus (house mouse) Hpgd NP_032304.2
Rattus norvegicus (Norway rat) Hpgd NP_077366.2
Gallus gallus (chicken) HPGD NP_001264713.1
Danio rerio (zebrafish) LOC565975 XP_694331.5
Xenopus (Silurana) tropicalis (western clawed frog) hpgd NP_001007992.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HPGD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HPGD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu58309 XM_011531907 PREDICTED: Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu16914 NM_001256306 Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu16895
NM_001256301 Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu16860 NM_001145816 Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu16895
NM_001256307 Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu16712 NM_001256305 Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu20277
NM_000860 Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu58309
Clone ID Related Accession (Same CDS sequence) XM_011531907
Accession Version XM_011531907.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 531bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_016354.20) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
ATGCACGTGA ACGGCAAAGT GGCGCTGGTG ACCGGCGCGG CTCAGGGCAT AGGCAGAGCC 
TTTGCAGAGG CGCTGCTGCT TAAGGGCGCC AAGGTAGCGC TGGTGGATTG GAATCTTGAA
GCAGGTGTAC AGTGTAAAGC TGCCCTGGAT GAGCAGTTTG AACCTCAGAA GACTCTGTTC
ATCCAGTGCG ATGTGGCTGA CCAGCAACAA CTGAGAGACA CTTTTAGAAA AGTTGTAGAC
CACTTTGGAA GACTGGACAT TTTGGTCAAT AATGCTGGAG TGAATAATGA GAAAAACTGG
GAAAAAACTC TGCAAATTAA TTTGGTTTCT GTTATCAGTG GAACCTATCT TGGTTTGGAT
TACATGAGTA AGCAAAATGG AGGTGAAGGC GGCATCATTA TCAATATGTC ATCTTTAGCA
GGTATCTCTT CTGTATGCAG ACATTTGAAA ACCTGGCTGG CAGAAGTTCT GCCGTCTTCA
GCAGGGAGCT CCCAGGGTTA CTTTAGAACT GCTGGATTCC TTTTTCTCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011530209.1
CDS443..973
Misc Feature(1)458..>865(+)
Misc Feature(2)476..856(+)
Misc Feature(3)761..856(+)
Translation

Target ORF information:

RefSeq Version XM_011531907
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531907

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGCACGTGA ACGGCAAAGT GGCGCTGGTG ACCGGCGCGG CTCAGGGCAT AGGCAGAGCC 
TTTGCAGAGG CGCTGCTGCT TAAGGGCGCC AAGGTAGCGC TGGTGGATTG GAATCTTGAA
GCAGGTGTAC AGTGTAAAGC TGCCCTGGAT GAGCAGTTTG AACCTCAGAA GACTCTGTTC
ATCCAGTGCG ATGTGGCTGA CCAGCAACAA CTGAGAGACA CTTTTAGAAA AGTTGTAGAC
CACTTTGGAA GACTGGACAT TTTGGTCAAT AATGCTGGAG TGAATAATGA GAAAAACTGG
GAAAAAACTC TGCAAATTAA TTTGGTTTCT GTTATCAGTG GAACCTATCT TGGTTTGGAT
TACATGAGTA AGCAAAATGG AGGTGAAGGC GGCATCATTA TCAATATGTC ATCTTTAGCA
GGTATCTCTT CTGTATGCAG ACATTTGAAA ACCTGGCTGG CAGAAGTTCT GCCGTCTTCA
GCAGGGAGCT CCCAGGGTTA CTTTAGAACT GCTGGATTCC TTTTTCTCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16914
Clone ID Related Accession (Same CDS sequence) NM_001256306
Accession Version NM_001256306.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 597bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-APR-2015
Organism Homo sapiens (human)
Product 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC420578.1, AK300125.1, BC018986.2 and AI435471.1. Transcript Variant: This variant (5) lacks two alternate in-frame exons in the coding region, compared to variant 1. It encodes isoform 5, which is shorter but contains the same N- and C-termini, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300125.1, AU139547.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
ATGCACGTGA ACGGCAAAGT GGCGCTGGTG ACCGGCGCGG CTCAGGGCAT AGGCAGAGCC 
TTTGCAGAGG CGCTGCTGCT TAAGGGCGCC AAGGTAGCGC TGGTGGATTG GAATCTTGAA
GCAGGTGTAC AGTGTAAAGC TGCCCTGGAT GAGCAGTTTG AACCTCAGAA GACTCTGTTC
ATCCAGTGCG ATGTGGCTGA CCAGCAACAA CTGAGAGGAC TCATGCCCGT TGCACAGCAG
CCGGTTTATT GTGCTTCAAA GCATGGCATA GTTGGATTCA CACGCTCAGC AGCGTTGGCT
GCTAATCTTA TGAACAGTGG TGTGAGACTG AATGCCATTT GTCCAGGCTT TGTTAACACA
GCCATCCTTG AATCAATTGA AAAAGAAGAA AACATGGGAC AATATATAGA ATATAAGGAT
CATATCAAGG ATATGATTAA ATACTATGGA ATTTTGGACC CACCATTGAT TGCCAATGGA
TTGATAACAC TCATTGAAGA TGATGCTTTA AATGGTGCTA TTATGAAGAT CACAACTTCT
AAGGGAATTC ATTTTCAAGA CTATGATACA ACTCCATTTC AAGCAAAAAC CCAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001243235.1
CDS448..1044
Misc Feature(1)277..279(+)
Misc Feature(2)448..978(+)
Misc Feature(3)463..1005(+)
Misc Feature(4)481..801(+)
Misc Feature(5)694..708(+)
Exon (1)1..540
Gene:HPGD
Gene Synonym:
Exon (2)541..664
Gene:HPGD
Gene Synonym:
Exon (3)665..741
Gene:HPGD
Gene Synonym:
Exon (4)742..905
Gene:HPGD
Gene Synonym:
Exon (5)906..2823
Gene:HPGD
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001256306
Organism Homo sapiens (human)
Definition Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001256306

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGCACGTGA ACGGCAAAGT GGCGCTGGTG ACCGGCGCGG CTCAGGGCAT AGGCAGAGCC 
TTTGCAGAGG CGCTGCTGCT TAAGGGCGCC AAGGTAGCGC TGGTGGATTG GAATCTTGAA
GCAGGTGTAC AGTGTAAAGC TGCCCTGGAT GAGCAGTTTG AACCTCAGAA GACTCTGTTC
ATCCAGTGCG ATGTGGCTGA CCAGCAACAA CTGAGAGGAC TCATGCCCGT TGCACAGCAG
CCGGTTTATT GTGCTTCAAA GCATGGCATA GTTGGATTCA CACGCTCAGC AGCGTTGGCT
GCTAATCTTA TGAACAGTGG TGTGAGACTG AATGCCATTT GTCCAGGCTT TGTTAACACA
GCCATCCTTG AATCAATTGA AAAAGAAGAA AACATGGGAC AATATATAGA ATATAAGGAT
CATATCAAGG ATATGATTAA ATACTATGGA ATTTTGGACC CACCATTGAT TGCCAATGGA
TTGATAACAC TCATTGAAGA TGATGCTTTA AATGGTGCTA TTATGAAGAT CACAACTTCT
AAGGGAATTC ATTTTCAAGA CTATGATACA ACTCCATTTC AAGCAAAAAC CCAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16895
Clone ID Related Accession (Same CDS sequence) NM_001256301 , NM_001256307
Accession Version NM_001256301.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 438bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-APR-2015
Organism Homo sapiens (human)
Product 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK296642.1, BC018986.2 and AI435471.1. On Jan 19, 2012 this sequence version replaced gi:224922793. Transcript Variant: This variant (3) differs in the 5' UTR and lacks a portion of the 5' coding region, compared to variant 1. These differences result in translation at a downstream start codon and an isoform (3) with a shorter N-terminus, compared to isoform 1. Variants 3 and 6 encode the same protein (isoform 3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296642.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
ATGAGTAAGC AAAATGGAGG TGAAGGCGGC ATCATTATCA ATATGTCATC TTTAGCAGGA 
CTCATGCCCG TTGCACAGCA GCCGGTTTAT TGTGCTTCAA AGCATGGCAT AGTTGGATTC
ACACGCTCAG CAGCGTTGGC TGCTAATCTT ATGAACAGTG GTGTGAGACT GAATGCCATT
TGTCCAGGCT TTGTTAACAC AGCCATCCTT GAATCAATTG AAAAAGAAGA AAACATGGGA
CAATATATAG AATATAAGGA TCATATCAAG GATATGATTA AATACTATGG AATTTTGGAC
CCACCATTGA TTGCCAATGG ATTGATAACA CTCATTGAAG ATGATGCTTT AAATGGTGCT
ATTATGAAGA TCACAACTTC TAAGGGAATT CATTTTCAAG ACTATGATAC AACTCCATTT
CAAGCAAAAA CCCAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001243230.1
CDS541..978
Misc Feature(1)190..192(+)
Misc Feature(2)541..939(+)
Misc Feature(3)589..642(+)
Exon (1)1..270
Gene:HPGD
Gene Synonym:
Exon (2)271..394
Gene:HPGD
Gene Synonym:
Exon (3)395..501
Gene:HPGD
Gene Synonym:
Exon (4)502..598
Gene:HPGD
Gene Synonym:
Exon (5)599..675
Gene:HPGD
Gene Synonym:
Exon (6)676..839
Gene:HPGD
Gene Synonym:
Exon (7)840..2757
Gene:HPGD
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001256301
Organism Homo sapiens (human)
Definition Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001256301

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGAGTAAGC AAAATGGAGG TGAAGGCGGC ATCATTATCA ATATGTCATC TTTAGCAGGA 
CTCATGCCCG TTGCACAGCA GCCGGTTTAT TGTGCTTCAA AGCATGGCAT AGTTGGATTC
ACACGCTCAG CAGCGTTGGC TGCTAATCTT ATGAACAGTG GTGTGAGACT GAATGCCATT
TGTCCAGGCT TTGTTAACAC AGCCATCCTT GAATCAATTG AAAAAGAAGA AAACATGGGA
CAATATATAG AATATAAGGA TCATATCAAG GATATGATTA AATACTATGG AATTTTGGAC
CCACCATTGA TTGCCAATGG ATTGATAACA CTCATTGAAG ATGATGCTTT AAATGGTGCT
ATTATGAAGA TCACAACTTC TAAGGGAATT CATTTTCAAG ACTATGATAC AACTCCATTT
CAAGCAAAAA CCCAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16860
Clone ID Related Accession (Same CDS sequence) NM_001145816
Accession Version NM_001145816.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 537bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-APR-2015
Organism Homo sapiens (human)
Product 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC420578.1, X82460.1, AK300940.1, BC018986.2 and AI435471.1. On Jan 19, 2012 this sequence version replaced gi:224922800. Transcript Variant: This variant (2) lacks an alternate exon in the 3' coding region resulting in a frameshift, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300940.1, X82460.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGCACGTGA ACGGCAAAGT GGCGCTGGTG ACCGGCGCGG CTCAGGGCAT AGGCAGAGCC 
TTTGCAGAGG CGCTGCTGCT TAAGGGCGCC AAGGTAGCGC TGGTGGATTG GAATCTTGAA
GCAGGTGTAC AGTGTAAAGC TGCCCTGGAT GAGCAGTTTG AACCTCAGAA GACTCTGTTC
ATCCAGTGCG ATGTGGCTGA CCAGCAACAA CTGAGAGACA CTTTTAGAAA AGTTGTAGAC
CACTTTGGAA GACTGGACAT TTTGGTCAAT AATGCTGGAG TGAATAATGA GAAAAACTGG
GAAAAAACTC TGCAAATTAA TTTGGTTTCT GTTATCAGTG GAACCTATCT TGGTTTGGAT
TACATGAGTA AGCAAAATGG AGGTGAAGGC GGCATCATTA TCAATATGTC ATCTTTAGCA
GGACTCATGC CCGTTGCACA GCAGCCGGTT TATTGTGCTT CAAAGCATGG CATAGTTGGA
TTCACACGCT CAGCAGCGCC CACCATTGAT TGCCAATGGA TTGATAACAC TCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001139288.1
CDS448..984
Misc Feature(1)277..279(+)
Misc Feature(2)463..>951(+)
Misc Feature(3)463..945(+)
Misc Feature(4)481..912(+)
Misc Feature(5)745..951(+)
Misc Feature(6)766..912(+)
Exon (1)1..540
Gene:HPGD
Gene Synonym:
Exon (2)541..664
Gene:HPGD
Gene Synonym:
Exon (3)665..771
Gene:HPGD
Gene Synonym:
Exon (4)772..868
Gene:HPGD
Gene Synonym:
Exon (5)869..945
Gene:HPGD
Gene Synonym:
Exon (6)946..2863
Gene:HPGD
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001145816
Organism Homo sapiens (human)
Definition Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001145816

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGCACGTGA ACGGCAAAGT GGCGCTGGTG ACCGGCGCGG CTCAGGGCAT AGGCAGAGCC 
TTTGCAGAGG CGCTGCTGCT TAAGGGCGCC AAGGTAGCGC TGGTGGATTG GAATCTTGAA
GCAGGTGTAC AGTGTAAAGC TGCCCTGGAT GAGCAGTTTG AACCTCAGAA GACTCTGTTC
ATCCAGTGCG ATGTGGCTGA CCAGCAACAA CTGAGAGACA CTTTTAGAAA AGTTGTAGAC
CACTTTGGAA GACTGGACAT TTTGGTCAAT AATGCTGGAG TGAATAATGA GAAAAACTGG
GAAAAAACTC TGCAAATTAA TTTGGTTTCT GTTATCAGTG GAACCTATCT TGGTTTGGAT
TACATGAGTA AGCAAAATGG AGGTGAAGGC GGCATCATTA TCAATATGTC ATCTTTAGCA
GGACTCATGC CCGTTGCACA GCAGCCGGTT TATTGTGCTT CAAAGCATGG CATAGTTGGA
TTCACACGCT CAGCAGCGCC CACCATTGAT TGCCAATGGA TTGATAACAC TCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16895
Clone ID Related Accession (Same CDS sequence) NM_001256301 , NM_001256307
Accession Version NM_001256307.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 438bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-APR-2015
Organism Homo sapiens (human)
Product 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC420578.1, AK300524.1, AC096751.3, BC018986.2 and AI435471.1. Transcript Variant: This variant (6) lacks a 5' exon compared to variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a weak Kozak sequence and a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG, to encode an isoform (3) with a shorter N-terminus, compared to isoform 1. Variants 3 and 6 encode the same protein (isoform 3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300524.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
ATGAGTAAGC AAAATGGAGG TGAAGGCGGC ATCATTATCA ATATGTCATC TTTAGCAGGA 
CTCATGCCCG TTGCACAGCA GCCGGTTTAT TGTGCTTCAA AGCATGGCAT AGTTGGATTC
ACACGCTCAG CAGCGTTGGC TGCTAATCTT ATGAACAGTG GTGTGAGACT GAATGCCATT
TGTCCAGGCT TTGTTAACAC AGCCATCCTT GAATCAATTG AAAAAGAAGA AAACATGGGA
CAATATATAG AATATAAGGA TCATATCAAG GATATGATTA AATACTATGG AATTTTGGAC
CCACCATTGA TTGCCAATGG ATTGATAACA CTCATTGAAG ATGATGCTTT AAATGGTGCT
ATTATGAAGA TCACAACTTC TAAGGGAATT CATTTTCAAG ACTATGATAC AACTCCATTT
CAAGCAAAAA CCCAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001243236.1
CDS704..1141
Misc Feature(1)659..661(+)
Misc Feature(2)704..1102(+)
Misc Feature(3)752..805(+)
Exon (1)1..540
Gene:HPGD
Gene Synonym:
Exon (2)541..664
Gene:HPGD
Gene Synonym:
Exon (3)665..761
Gene:HPGD
Gene Synonym:
Exon (4)762..838
Gene:HPGD
Gene Synonym:
Exon (5)839..1002
Gene:HPGD
Gene Synonym:
Exon (6)1003..2920
Gene:HPGD
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001256307
Organism Homo sapiens (human)
Definition Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001256307

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGAGTAAGC AAAATGGAGG TGAAGGCGGC ATCATTATCA ATATGTCATC TTTAGCAGGA 
CTCATGCCCG TTGCACAGCA GCCGGTTTAT TGTGCTTCAA AGCATGGCAT AGTTGGATTC
ACACGCTCAG CAGCGTTGGC TGCTAATCTT ATGAACAGTG GTGTGAGACT GAATGCCATT
TGTCCAGGCT TTGTTAACAC AGCCATCCTT GAATCAATTG AAAAAGAAGA AAACATGGGA
CAATATATAG AATATAAGGA TCATATCAAG GATATGATTA AATACTATGG AATTTTGGAC
CCACCATTGA TTGCCAATGG ATTGATAACA CTCATTGAAG ATGATGCTTT AAATGGTGCT
ATTATGAAGA TCACAACTTC TAAGGGAATT CATTTTCAAG ACTATGATAC AACTCCATTT
CAAGCAAAAA CCCAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16712
Clone ID Related Accession (Same CDS sequence) NM_001256305
Accession Version NM_001256305.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 432bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-APR-2015
Organism Homo sapiens (human)
Product 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC420578.1, U63296.1, AK058013.1, BC018986.2 and AI435471.1. Transcript Variant: This variant (4) lacks two alternate exons in the 3' coding region, which results in a frameshift and early stop codon, compared to variant 1. It encodes isoform 4, which is shorter and contains a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U63296.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2161836 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
ATGCACGTGA ACGGCAAAGT GGCGCTGGTG ACCGGCGCGG CTCAGGGCAT AGGCAGAGCC 
TTTGCAGAGG CGCTGCTGCT TAAGGGCGCC AAGGTAGCGC TGGTGGATTG GAATCTTGAA
GCAGGTGTAC AGTGTAAAGC TGCCCTGGAT GAGCAGTTTG AACCTCAGAA GACTCTGTTC
ATCCAGTGCG ATGTGGCTGA CCAGCAACAA CTGAGAGACA CTTTTAGAAA AGTTGTAGAC
CACTTTGGAA GACTGGACAT TTTGGTCAAT AATGCTGGAG TGAATAATGA GAAAAACTGG
GAAAAAACTC TGCAAATTAA TTTGGTTTCT GTTATCAGTG GAACCTATCT TGGTTTGGAT
TACATGAGTA AGCAAAATGG AGGTGAAGGC GGCATCATTA TCAATATGTC ATCTTTAGCA
GCCCACCATT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001243234.1
CDS448..879
Misc Feature(1)277..279(+)
Misc Feature(2)463..>876(+)
Misc Feature(3)481..861(+)
Misc Feature(4)766..861(+)
Exon (1)1..540
Gene:HPGD
Gene Synonym:
Exon (2)541..664
Gene:HPGD
Gene Synonym:
Exon (3)665..771
Gene:HPGD
Gene Synonym:
Exon (4)772..868
Gene:HPGD
Gene Synonym:
Exon (5)869..2786
Gene:HPGD
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001256305
Organism Homo sapiens (human)
Definition Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001256305

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGCACGTGA ACGGCAAAGT GGCGCTGGTG ACCGGCGCGG CTCAGGGCAT AGGCAGAGCC 
TTTGCAGAGG CGCTGCTGCT TAAGGGCGCC AAGGTAGCGC TGGTGGATTG GAATCTTGAA
GCAGGTGTAC AGTGTAAAGC TGCCCTGGAT GAGCAGTTTG AACCTCAGAA GACTCTGTTC
ATCCAGTGCG ATGTGGCTGA CCAGCAACAA CTGAGAGACA CTTTTAGAAA AGTTGTAGAC
CACTTTGGAA GACTGGACAT TTTGGTCAAT AATGCTGGAG TGAATAATGA GAAAAACTGG
GAAAAAACTC TGCAAATTAA TTTGGTTTCT GTTATCAGTG GAACCTATCT TGGTTTGGAT
TACATGAGTA AGCAAAATGG AGGTGAAGGC GGCATCATTA TCAATATGTC ATCTTTAGCA
GCCCACCATT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20277
Clone ID Related Accession (Same CDS sequence) NM_000860
Accession Version NM_000860.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 801bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-APR-2015
Organism Homo sapiens (human)
Product 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC420578.1, BC018986.2 and AI435471.1. This sequence is a reference standard in the RefSeqGene project. On Jan 19, 2012 this sequence version replaced gi:224922792. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK058013.1, BC018986.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCACGTGA ACGGCAAAGT GGCGCTGGTG ACCGGCGCGG CTCAGGGCAT AGGCAGAGCC 
TTTGCAGAGG CGCTGCTGCT TAAGGGCGCC AAGGTAGCGC TGGTGGATTG GAATCTTGAA
GCAGGTGTAC AGTGTAAAGC TGCCCTGGAT GAGCAGTTTG AACCTCAGAA GACTCTGTTC
ATCCAGTGCG ATGTGGCTGA CCAGCAACAA CTGAGAGACA CTTTTAGAAA AGTTGTAGAC
CACTTTGGAA GACTGGACAT TTTGGTCAAT AATGCTGGAG TGAATAATGA GAAAAACTGG
GAAAAAACTC TGCAAATTAA TTTGGTTTCT GTTATCAGTG GAACCTATCT TGGTTTGGAT
TACATGAGTA AGCAAAATGG AGGTGAAGGC GGCATCATTA TCAATATGTC ATCTTTAGCA
GGACTCATGC CCGTTGCACA GCAGCCGGTT TATTGTGCTT CAAAGCATGG CATAGTTGGA
TTCACACGCT CAGCAGCGTT GGCTGCTAAT CTTATGAACA GTGGTGTGAG ACTGAATGCC
ATTTGTCCAG GCTTTGTTAA CACAGCCATC CTTGAATCAA TTGAAAAAGA AGAAAACATG
GGACAATATA TAGAATATAA GGATCATATC AAGGATATGA TTAAATACTA TGGAATTTTG
GACCCACCAT TGATTGCCAA TGGATTGATA ACACTCATTG AAGATGATGC TTTAAATGGT
GCTATTATGA AGATCACAAC TTCTAAGGGA ATTCATTTTC AAGACTATGA TACAACTCCA
TTTCAAGCAA AAACCCAATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000851.2
CDS448..1248
Misc Feature(1)277..279(+)
Misc Feature(2)448..1182(+)
Misc Feature(3)463..1209(+)
Misc Feature(4)481..1020(+)
Misc Feature(5)745..1209(+)
Misc Feature(6)766..912(+)
Exon (1)1..540
Gene:HPGD
Gene Synonym:
Exon (2)541..664
Gene:HPGD
Gene Synonym:
Exon (3)665..771
Gene:HPGD
Gene Synonym:
Exon (4)772..868
Gene:HPGD
Gene Synonym:
Exon (5)869..945
Gene:HPGD
Gene Synonym:
Exon (6)946..1109
Gene:HPGD
Gene Synonym:
Exon (7)1110..3027
Gene:HPGD
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000860
Organism Homo sapiens (human)
Definition Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000860

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCACGTGA ACGGCAAAGT GGCGCTGGTG ACCGGCGCGG CTCAGGGCAT AGGCAGAGCC 
TTTGCAGAGG CGCTGCTGCT TAAGGGCGCC AAGGTAGCGC TGGTGGATTG GAATCTTGAA
GCAGGTGTAC AGTGTAAAGC TGCCCTGGAT GAGCAGTTTG AACCTCAGAA GACTCTGTTC
ATCCAGTGCG ATGTGGCTGA CCAGCAACAA CTGAGAGACA CTTTTAGAAA AGTTGTAGAC
CACTTTGGAA GACTGGACAT TTTGGTCAAT AATGCTGGAG TGAATAATGA GAAAAACTGG
GAAAAAACTC TGCAAATTAA TTTGGTTTCT GTTATCAGTG GAACCTATCT TGGTTTGGAT
TACATGAGTA AGCAAAATGG AGGTGAAGGC GGCATCATTA TCAATATGTC ATCTTTAGCA
GGACTCATGC CCGTTGCACA GCAGCCGGTT TATTGTGCTT CAAAGCATGG CATAGTTGGA
TTCACACGCT CAGCAGCGTT GGCTGCTAAT CTTATGAACA GTGGTGTGAG ACTGAATGCC
ATTTGTCCAG GCTTTGTTAA CACAGCCATC CTTGAATCAA TTGAAAAAGA AGAAAACATG
GGACAATATA TAGAATATAA GGATCATATC AAGGATATGA TTAAATACTA TGGAATTTTG
GACCCACCAT TGATTGCCAA TGGATTGATA ACACTCATTG AAGATGATGC TTTAAATGGT
GCTATTATGA AGATCACAAC TTCTAAGGGA ATTCATTTTC AAGACTATGA TACAACTCCA
TTTCAAGCAA AAACCCAATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
*
*
*
*