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ERAS cDNA ORF clone, Homo sapiens (human)

Gene Symbol ERAS
Entrez Gene ID 3266
Full Name ES cell expressed Ras
Synonyms HRAS2, HRASP
General protein information
Preferred Names
GTPase ERas
Names
GTPase ERas
E-Ras
small GTPase protein E-Ras
embryonic stem cell-expressed Ras
v-Ha-ras Harvey rat sarcoma viral oncogene homolog 2
v-Ha-ras Harvey rat sarcoma viral oncogene homolog pseudogene
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xp11.23

Summary This gene encodes a constitutively active member of the small GTPase Ras protein family. The encoded protein activates the phosphatidylinositol 3-kinase signal transduction pathway in undifferentiated stem cells, but is not expressed in differentiated cells. This gene may be involved in cancer and chemotherapy resistance. [provided by RefSeq, Dec 2012]. lac of sum
Disorder MIM:

300437


mRNA and Protein(s)

mRNA Protein Name
NM_181532 NP_853510 GTPase ERas precursor


Homo sapiens (human) ERAS NP_853510.1
Macaca mulatta (Rhesus monkey) ERAS XP_001101805.1
Canis lupus familiaris (dog) ERAS XP_548988.1
Bos taurus (cattle) ERAS XP_002700122.2
Mus musculus (house mouse) Eras NP_853526.1
Rattus norvegicus (Norway rat) Eras NP_001102845.1
Rattus norvegicus (Norway rat) LOC100911243 XP_003752059.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ERAS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ERAS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu07581 NM_181532 Homo sapiens ES cell expressed Ras (ERAS), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu07581
Clone ID Related Accession (Same CDS sequence) NM_181532
Accession Version NM_181532.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 702bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product GTPase ERas precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY043532.1 and BC101642.1. On Dec 5, 2012 this sequence version replaced gi:31581555. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY043532.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGAGCTGC CAACAAAGCC TGGCACCTTC GACCTGGGCC TGGCCACATG GAGCCCTTCC 
TTCCAGGGGG AAACCCACCG GGCTCAGGCA CGCCGCAGGG ATGTTGGCAG GCAGCTGCCT
GAGTACAAGG CTGTGGTGGT GGGCGCCAGT GGCGTGGGCA AGAGTGCGCT GACCATCCAG
CTGAACCACC AGTGCTTCGT GGAGGACCAC GACCCCACCA TCCAGGATTC CTACTGGAAG
GAGTTGACCC TGGACAGTGG GGACTGCATT CTGAATGTGC TGGACACAGC AGGGCAGGCC
ATCCATAGGG CCCTGCGTGA CCAGTGCCTG GCTGTCTGTG ATGGTGTGCT GGGCGTCTTC
GCTCTCGATG ACCCCTCGTC TCTGATCCAG CTGCAGCAGA TATGGGCCAC CTGGGGCCCT
CACCCCGCCC AGCCCCTTGT CCTCGTGGGC AACAAGTGTG ACCTTGTGAC CACTGCTGGA
GATGCTCATG CCGCTGCTGC AGCCCTCGCA CACAGCTGGG GGGCCCACTT CGTGGAGACC
TCGGCCAAAA CACGGCAAGG CGTGGAGGAG GCCTTTTCCC TGCTGGTCCA TGAGATCCAG
AGGGTCCAGG AGGCCATGGC GAAGGAGCCC ATGGCAAGGT CCTGTAGGGA GAAGACCCGG
CACCAGAAGG CCACCTGCCA CTGTGGCTGC TCTGTGGCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_853510.1
CDS81..782
Misc Feature(1)201..746(+)
Misc Feature(2)204..683(+)
Misc Feature(3)222..245(+)
Misc Feature(4)225..491(+)
Misc Feature(5)228..626(+)
Misc Feature(6)243..629(+)
Misc Feature(7)267..647(+)
Misc Feature(8)288..314(+)
Misc Feature(9)291..314(+)
Misc Feature(10)297..299(+)
Misc Feature(11)363..374(+)
Misc Feature(12)369..425(+)
Misc Feature(13)531..542(+)
Misc Feature(14)567..617(+)
Misc Feature(15)621..629(+)
Exon (1)1..37
Gene:ERAS
Gene Synonym:
Exon (2)38..826
Gene:ERAS
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_181532
Organism Homo sapiens (human)
Definition Homo sapiens ES cell expressed Ras (ERAS), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_181532

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGAGCTGC CAACAAAGCC TGGCACCTTC GACCTGGGCC TGGCCACATG GAGCCCTTCC 
TTCCAGGGGG AAACCCACCG GGCTCAGGCA CGCCGCAGGG ATGTTGGCAG GCAGCTGCCT
GAGTACAAGG CTGTGGTGGT GGGCGCCAGT GGCGTGGGCA AGAGTGCGCT GACCATCCAG
CTGAACCACC AGTGCTTCGT GGAGGACCAC GACCCCACCA TCCAGGATTC CTACTGGAAG
GAGTTGACCC TGGACAGTGG GGACTGCATT CTGAATGTGC TGGACACAGC AGGGCAGGCC
ATCCATAGGG CCCTGCGTGA CCAGTGCCTG GCTGTCTGTG ATGGTGTGCT GGGCGTCTTC
GCTCTCGATG ACCCCTCGTC TCTGATCCAG CTGCAGCAGA TATGGGCCAC CTGGGGCCCT
CACCCCGCCC AGCCCCTTGT CCTCGTGGGC AACAAGTGTG ACCTTGTGAC CACTGCTGGA
GATGCTCATG CCGCTGCTGC AGCCCTCGCA CACAGCTGGG GGGCCCACTT CGTGGAGACC
TCGGCCAAAA CACGGCAAGG CGTGGAGGAG GCCTTTTCCC TGCTGGTCCA TGAGATCCAG
AGGGTCCAGG AGGCCATGGC GAAGGAGCCC ATGGCAAGGT CCTGTAGGGA GAAGACCCGG
CACCAGAAGG CCACCTGCCA CTGTGGCTGC TCTGTGGCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Role of the ERas gene in gastric cancer cells
Oncol. Rep. 30 (1), 50-56 (2013)
Liu Y, Wang Z, Li H, Wu Z, Wei F and Wang H.


book

Functional genomics identifies drivers of medulloblastoma dissemination
Cancer Res. 72 (19), 4944-4953 (2012)
Mumert M, Dubuc A, Wu X, Northcott PA, Chin SS, Pedone CA, Taylor MD and Fults DW.


book

ERas enhances resistance to CPT-11 in gastric cancer
Anticancer Res. 31 (10), 3353-3360 (2011)
Kubota E, Kataoka H, Tanaka M, Okamoto Y, Ebi M, Hirata Y, Murakami K, Mizoshita T, Shimura T, Mori Y, Tanida S, Kamiya T, Aoyama M, Asai K and Joh T.


book

Identification of HLA-A*2402-restricted epitope peptide derived from ERas oncogene expressed in human scirrhous gastric cancer
Cancer Sci. 102 (4), 683-689 (2011)
Iwauchi T, Tanaka H, Yamazoe S, Yashiro M, Yoshii M, Kubo N, Muguruma K, Sawada T, Ohira M and Hirakawa K.


book

Detection of beta-catenin, gastrokine-2 and embryonic stem cell expressed ras in gastric cancers
Int J Clin Exp Pathol 3 (8), 782-791 (2010)
Zhang F, Tang JM, Wang L, Shen JY, Zheng L, Wu PP, Zhang M and Yan ZW.


book

A novel rasH2 mouse carcinogenesis model that is highly susceptible to 4-NQO-induced tongue and esophageal carcinogenesis is useful for preclinical chemoprevention studies
Carcinogenesis 29 (2), 418-426 (2008)
Miyamoto S, Yasui Y, Kim M, Sugie S, Murakami A, Ishigamori-Suzuki R and Tanaka T.


book

ERas oncogene expression and epigenetic regulation by histone acetylation in human cancer cells
Anticancer Res. 27 (6B), 4071-4075 (2007)
Yasuda K, Yashiro M, Sawada T, Ohira M and Hirakawa K.


book

Human ERas gene has an upstream premature polyadenylation signal that results in a truncated, noncoding transcript
Stem Cells 23 (10), 1535-1540 (2005)
Kameda T and Thomson JA.


book

Role of ERas in promoting tumour-like properties in mouse embryonic stem cells
Nature 423 (6939), 541-545 (2003)
Takahashi K, Mitsui K and Yamanaka S.


book

The human c-Ha-ras2 is a processed pseudogene inactivated by numerous base substitutions
Nucleic Acids Res. 12 (4), 1821-1828 (1984)
Miyoshi,J., Kagimoto,M., Soeda,E. and Sakaki,Y.


 
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