Email to GenScript

HSD3B2 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 [Homo sapiens (human)]

Gene Symbol HSD3B2
Entrez Gene ID 3284
Full Name hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
Synonyms HSD3B, HSDB, SDR11E2
General protein information
Preferred Names
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Names
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
3-beta-HSD II
3 beta-HSD type II
progesterone reductase
delta 5-delta 4-isomerase type II
3-beta-HSD adrenal and gonadal type
3-beta-hydroxy-5-ene steroid dehydrogenase
3-beta-hydroxy-Delta(5)-steroid dehydrogenase
short chain dehydrogenase/reductase family 11E, member 2
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II
3 beta-hydroxysteroid dehydrogenase type II, delta 5-delta 4-isomerase type II, 3 beta-HSD type II
NP_000189.1
NP_001159592.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1p13.1

Summary The protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]. lac of sum
Disorder MIM:

613890

Disorder Html:





The following HSD3B2 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSD3B2 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu14034 NM_000198 Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (HSD3B2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$379
OHu14034 NM_001166120 Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (HSD3B2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$379

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu14034
Accession Version NM_000198.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1119bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AV705363.1, BC038419.1 and AK222997.1. On Oct 8, 2009 this sequence version replaced gi:50726973. Summary: The protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]. Transcript Variant: This variant (1) represents the predominant transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222997.1, BC038419.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGGCTGGA GCTGCCTTGT GACAGGAGCA GGAGGGCTTC TGGGTCAGAG GATCGTCCGC 
CTGTTGGTGG AAGAGAAGGA ACTGAAGGAG ATCAGGGCCT TGGACAAGGC CTTCAGACCA
GAATTGAGAG AGGAATTTTC TAAGCTCCAG AACAGGACCA AGCTGACTGT ACTTGAAGGA
GACATTCTGG ATGAGCCATT CCTGAAAAGA GCCTGCCAGG ACGTCTCGGT CGTCATCCAC
ACCGCCTGTA TCATTGATGT CTTTGGTGTC ACTCACAGAG AGTCCATCAT GAATGTCAAT
GTGAAAGGTA CCCAGCTACT GTTGGAGGCC TGTGTCCAAG CCAGTGTGCC AGTCTTCATC
TACACCAGTA GCATAGAGGT AGCCGGGCCC AACTCCTACA AGGAAATCAT CCAGAACGGC
CACGAAGAAG AGCCTCTGGA AAACACATGG CCCACTCCAT ACCCGTACAG CAAAAAGCTT
GCTGAGAAGG CTGTGCTGGC GGCTAATGGG TGGAATCTAA AAAATGGTGA TACCTTGTAC
ACTTGTGCGT TAAGACCCAC ATATATCTAT GGGGAAGGAG GCCCATTCCT TTCTGCCAGT
ATAAATGAGG CCCTGAACAA CAATGGGATC CTGTCAAGTG TTGGAAAGTT CTCTACAGTC
AACCCAGTCT ATGTTGGCAA CGTGGCCTGG GCCCACATTC TGGCCTTGAG GGCTCTGCGG
GACCCCAAGA AGGCCCCAAG TGTCCGAGGT CAATTCTATT ACATCTCAGA TGACACGCCT
CACCAAAGCT ATGATAACCT TAATTACATC CTGAGCAAAG AGTTTGGCCT CCGCCTTGAT
TCCAGATGGA GCCTTCCTTT AACCCTGATG TACTGGATTG GCTTCCTGCT GGAAGTAGTG
AGCTTCCTAC TCAGCCCAAT TTACTCCTAT CAACCCCCCT TCAACCGCCA CACAGTCACA
TTATCAAATA GTGTGTTCAC CTTCTCTTAC AAGAAGGCTC AGCGAGATCT GGCGTATAAG
CCACTCTACA GCTGGGAGGA AGCCAAGCAG AAAACCGTGG AGTGGGTTGG TTCCCTTGTG
GACCGGCACA AGGAGACCCT GAAGTCCAAG ACTCAGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000189.1
CDS173..1291
Misc Feature(1)38..40(+)
Misc Feature(2)182..1240(+)
Misc Feature(3)188..1246(+)
Misc Feature(4)197..739(+)
Misc Feature(5)470..646(+)
Misc Feature(6)542..820(+)
Misc Feature(7)1031..1093(+)
Exon (1)1..83
Gene:HSD3B2
Gene Synonym:
Exon (2)84..314
Gene:HSD3B2
Gene Synonym:
Exon (3)315..479
Gene:HSD3B2
Gene Synonym:
Exon (4)480..1710
Gene:HSD3B2
Gene Synonym:
Translation
Position Chain Variation Link
28 28 a, g dbSNP:35226381
53 53 c, g dbSNP:587631566
67 67 c, t dbSNP:587688647
82 82 c, t dbSNP:150977514
123 123 g, t dbSNP:781632641
127 127 a, c, t dbSNP:748675518
130 130 c, t dbSNP:778397232
141 141 c, t dbSNP:6208
150 150 c, t dbSNP:771602997
168 168 c, g dbSNP:775250861
171 171 c, t dbSNP:187042846
172 172 a, c, g dbSNP:752085728
173 173 a, t dbSNP:776656223
177 177 g, t dbSNP:116449508
181 181 c, g dbSNP:765335418
183 183 a, g dbSNP:773416276
185 185 a, t dbSNP:376207606
187 187 a, c dbSNP:766474996
193 193 g, t dbSNP:751834454
200 200 g, t dbSNP:755248657
201 201 a, c dbSNP:28934880
205 205 a, g dbSNP:767881300
206 206 a, g dbSNP:753134237
207 207 a, g dbSNP:756607591
208 208 a, g dbSNP:778303029
212 212 c, g dbSNP:377504860
222 222 g, t dbSNP:748554689
223 223 a, g dbSNP:757957605
226 226 c, t dbSNP:777605448
227 227 a, g dbSNP:143169543
228 228 c, t dbSNP:115344376
230 230 c, t dbSNP:139191056
231 231 a, g dbSNP:748018110
232 232 c, g dbSNP:769828729
239 239 a, g dbSNP:773237182
250 250 g, t dbSNP:762899067
260 260 a, g dbSNP:753777808
268 268 a, g dbSNP:771061890
269 269 g, t dbSNP:774377636
274 274 a, g dbSNP:757051767
281 281 a, g dbSNP:759756499
282 282 c, g dbSNP:767793086
286 286 c, t dbSNP:753046362
288 288 c, g dbSNP:761068679
296 296 c, t dbSNP:370732845
298 298 -, ga dbSNP:760846678
299 299 a, g dbSNP:374785266
304 304 g, t dbSNP:111333222
317 317 a, c dbSNP:780947714
319 319 c, g dbSNP:752417564
321 321 a, g dbSNP:755912072
332 332 a, g dbSNP:372260820
337 337 a, g dbSNP:749102084
351 351 a, g dbSNP:141499927
369 369 c, t dbSNP:778763649
374 374 a, c dbSNP:138340684
379 379 a, g dbSNP:375679388
381 381 a, g dbSNP:772162284
385 385 c, t dbSNP:775633331
387 387 a, g, t dbSNP:747237944
392 392 a, g dbSNP:4986954
394 394 c, t dbSNP:150892928
395 395 a, g dbSNP:765748123
397 397 c, g dbSNP:773882717
399 399 c, t dbSNP:759419941
400 400 a, g dbSNP:767147330
403 403 c, t dbSNP:752329512
404 404 a, g dbSNP:143103963
410 410 c, t dbSNP:755849746
412 412 a, c dbSNP:763712349
415 415 c, t dbSNP:753645202
416 416 a, g dbSNP:757033996
418 418 c, t dbSNP:587717286
418 418 c, t dbSNP:778861971
422 422 a, g dbSNP:745820487
438 438 g, t dbSNP:368662017
449 449 a, g dbSNP:371800920
452 452 c, g dbSNP:6211
459 459 c, t dbSNP:767195449
473 473 c, g, t dbSNP:752453060
484 484 c, t dbSNP:367646123
500 500 a, g dbSNP:587730679
500 500 a, g dbSNP:781422494
508 508 c, t dbSNP:748328293
512 512 -, g dbSNP:765602803
515 515 a, g dbSNP:770127588
531 531 c, t dbSNP:778275898
538 538 c, t dbSNP:749726800
541 541 c, t dbSNP:758740855
556 556 c, t dbSNP:775037341
557 557 a, g dbSNP:587628683
557 557 a, g dbSNP:760184076
570 570 a, g dbSNP:768064787
582 582 a, t dbSNP:776281757
583 583 a, c dbSNP:374909270
589 589 c, t dbSNP:369303483
590 590 a, g dbSNP:587759526
590 590 a, g dbSNP:768416823
591 591 g, t dbSNP:142169766
592 592 c, t dbSNP:766267758
595 595 c, t dbSNP:751470493
596 596 a, g, t dbSNP:80358219
599 599 c, g dbSNP:587631086
599 599 c, g dbSNP:781133903
605 605 c, t dbSNP:752886470
608 608 c, t dbSNP:756389486
610 610 a, g dbSNP:587692614
626 626 a, g dbSNP:778188253
632 632 a, c, t dbSNP:749638934
636 636 c, t dbSNP:779418168
637 637 a, c, g dbSNP:114527791
638 638 c, t dbSNP:587676928
638 638 c, t dbSNP:775993749
663 663 c, t dbSNP:34562248
665 665 a, g dbSNP:761437204
672 672 c, t dbSNP:35486059
673 673 a, g dbSNP:6207
677 677 -, aatgggtgg dbSNP:750849889
684 684 a, g dbSNP:80358216
690 690 g, t dbSNP:762479018
697 697 c, t dbSNP:766170141
706 706 c, g dbSNP:751443478
712 712 a, c dbSNP:759383948
721 721 a, g dbSNP:33932258
722 722 c, t dbSNP:752695754
725 725 a, c dbSNP:756301579
727 727 -, c dbSNP:770815049
732 732 c, t dbSNP:764370917
734 734 c, t dbSNP:767943915
740 740 c, t dbSNP:754126326
742 742 c, t dbSNP:374075427
754 754 c, t dbSNP:757629697
765 765 c, t dbSNP:779142287
771 771 a, g dbSNP:377671756
779 779 a, g dbSNP:746303807
781 781 a, g dbSNP:375323828
783 783 c, t dbSNP:758811639
785 785 c, t dbSNP:138811555
787 787 a, g dbSNP:747620720
793 793 c, t dbSNP:143758913
797 797 a, g dbSNP:587669956
797 797 a, g dbSNP:772790725
800 800 a, g dbSNP:587741317
800 800 a, g dbSNP:748940918
802 802 c, t dbSNP:770633737
803 803 c, t dbSNP:143038843
809 809 a, g dbSNP:759422374
810 810 c, g dbSNP:137867568
820 820 g, t dbSNP:775527537
826 826 c, t dbSNP:760787616
829 829 a, t dbSNP:764274511
832 832 c, t dbSNP:754040085
836 836 a, c, g dbSNP:80358220
837 837 a, c dbSNP:765547422
841 841 c, t dbSNP:750725811
843 843 a, g dbSNP:150525350
844 844 c, t dbSNP:116728039
846 846 c, t dbSNP:747532631
849 849 a, g dbSNP:755550493
853 853 c, t dbSNP:587675471
853 853 c, t dbSNP:777257924
854 854 a, g dbSNP:748851282
860 860 c, t dbSNP:770464639
879 879 c, t dbSNP:35887327
886 886 c, t dbSNP:745608432
887 887 c, t dbSNP:771922495
890 890 c, t dbSNP:370603955
891 891 a, g dbSNP:373456025
892 892 a, g dbSNP:200212806
895 895 c, g dbSNP:776831616
896 896 c, t dbSNP:762026737
908 908 c, t dbSNP:587658718
908 908 c, t dbSNP:765340532
911 911 a, g dbSNP:750684493
914 914 aa, gt dbSNP:121964896
914 914 a, g dbSNP:763173486
917 917 c, t dbSNP:80358217
918 918 a, g dbSNP:751984843
922 922 c, t dbSNP:755461071
934 934 c, t dbSNP:781698731
942 942 a, c dbSNP:587734522
942 942 a, c dbSNP:753312765
948 948 c, t dbSNP:80358221
949 949 a, g dbSNP:33926546
950 950 c, t dbSNP:745547471
961 961 c, g, t dbSNP:771688849
980 980 a, g dbSNP:746851162
981 981 c, t dbSNP:75429891
987 987 c, g dbSNP:587772926
987 987 c, g dbSNP:776743549
989 989 -, aa dbSNP:754609778
990 990 a, g dbSNP:761754141
994 994 c, g dbSNP:769933669
1001 1001 c, t dbSNP:201384931
1004 1004 c, t dbSNP:587711475
1004 1004 c, t dbSNP:763227842
1005 1005 a, g dbSNP:766564863
1017 1017 a, g dbSNP:751897022
1024 1024 a, c, t dbSNP:151258756
1027 1027 a, t dbSNP:753224225
1039 1039 -, g dbSNP:767167623
1047 1047 a, g dbSNP:756663759
1057 1057 c, t dbSNP:778481893
1062 1062 c, t dbSNP:756147663
1063 1063 a, g dbSNP:779139349
1067 1067 a, g dbSNP:758045653
1081 1081 a, c, g dbSNP:757801056
1091 1091 a, g dbSNP:746758931
1096 1096 c, t dbSNP:587775183
1096 1096 c, t dbSNP:768590776
1103 1103 c, t dbSNP:781213951
1105 1105 a, c dbSNP:748116919
1118 1118 c, t dbSNP:114032180
1119 1119 a, g dbSNP:773331795
1123 1123 c, t dbSNP:762995520
1125 1125 c, t dbSNP:771113815
1132 1132 a, c dbSNP:34412477
1135 1135 a, g dbSNP:759846410
1141 1141 c, t dbSNP:767883215
1144 1144 c, t dbSNP:145137295
1147 1147 a, g dbSNP:761230660
1153 1153 a, c dbSNP:149441011
1158 1158 c, t dbSNP:749915716
1162 1162 c, t dbSNP:757875458
1164 1164 a, t dbSNP:766077589
1175 1175 a, c, t dbSNP:148200568
1179 1179 a, t dbSNP:781125519
1184 1184 a, g dbSNP:147383167
1185 1185 c, g, t dbSNP:756060240
1186 1186 a, g, t dbSNP:116342586
1194 1194 c, t dbSNP:121964897
1197 1197 g, t dbSNP:774500247
1198 1198 c, g dbSNP:746095411
1201 1201 c, t dbSNP:35849160
1204 1204 c, t dbSNP:772348263
1210 1210 a, g dbSNP:775901269
1216 1216 c, t dbSNP:760983984
1222 1222 a, g dbSNP:764620496
1227 1227 a, c dbSNP:777023732
1228 1228 c, g, t dbSNP:781770416
1229 1229 a, g dbSNP:143634775
1234 1234 a, g dbSNP:114064941
1236 1236 a, g dbSNP:767128094
1252 1252 g, t dbSNP:752508551
1256 1256 c, t dbSNP:199919404
1257 1257 a, g dbSNP:777718760
1269 1269 a, c dbSNP:749198869
1270 1270 c, t dbSNP:757261352
1285 1285 a, t dbSNP:778961069
1291 1291 a, c, g dbSNP:80358218
1295 1295 -, aggatgac dbSNP:752135021
1298 1298 a, g dbSNP:772250386
1303 1303 c, g dbSNP:775605483
1305 1305 a, g dbSNP:747348624
1310 1310 a, g dbSNP:768913118
1311 1311 g, t dbSNP:777126692
1312 1312 c, g dbSNP:762402613
1314 1314 a, g dbSNP:770477643
1319 1319 c, g dbSNP:587718678
1319 1319 c, g dbSNP:773980654
1323 1323 c, g, t dbSNP:759161114
1325 1325 g, t dbSNP:752317712
1326 1326 g, t dbSNP:760462265
1333 1333 a, c dbSNP:763802578
1336 1336 c, t dbSNP:753700611
1337 1337 c, t dbSNP:371081728
1338 1338 a, t dbSNP:374222089
1353 1353 c, t dbSNP:587599964
1387 1387 c, g dbSNP:9282703
1493 1493 a, g dbSNP:9282704
1498 1498 c, t dbSNP:186430448
1518 1518 g, t dbSNP:587653430
1560 1560 g, t dbSNP:191329987
1567 1567 cttaac, t dbSNP:386635104
1567 1567 c, t dbSNP:1819698
1570 1570 -, aactt dbSNP:369148049
1573 1573 c, t dbSNP:587679988
1596 1596 c, g dbSNP:587758091
1606 1606 -, c dbSNP:754135008
1612 1612 c, g dbSNP:1361530
1644 1644 c, t dbSNP:587707312
1645 1645 c, t dbSNP:747877605
1658 1658 a, g dbSNP:769352247
1688 1688 a, t dbSNP:753468192

Target ORF information:

RefSeq Version NM_000198
Organism Homo sapiens (human)
Definition Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (HSD3B2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000198

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGGCTGGA GCTGCCTTGT GACAGGAGCA GGAGGGCTTC TGGGTCAGAG GATCGTCCGC 
CTGTTGGTGG AAGAGAAGGA ACTGAAGGAG ATCAGGGCCT TGGACAAGGC CTTCAGACCA
GAATTGAGAG AGGAATTTTC TAAGCTCCAG AACAGGACCA AGCTGACTGT ACTTGAAGGA
GACATTCTGG ATGAGCCATT CCTGAAAAGA GCCTGCCAGG ACGTCTCGGT CGTCATCCAC
ACCGCCTGTA TCATTGATGT CTTTGGTGTC ACTCACAGAG AGTCCATCAT GAATGTCAAT
GTGAAAGGTA CCCAGCTACT GTTGGAGGCC TGTGTCCAAG CCAGTGTGCC AGTCTTCATC
TACACCAGTA GCATAGAGGT AGCCGGGCCC AACTCCTACA AGGAAATCAT CCAGAACGGC
CACGAAGAAG AGCCTCTGGA AAACACATGG CCCACTCCAT ACCCGTACAG CAAAAAGCTT
GCTGAGAAGG CTGTGCTGGC GGCTAATGGG TGGAATCTAA AAAATGGTGA TACCTTGTAC
ACTTGTGCGT TAAGACCCAC ATATATCTAT GGGGAAGGAG GCCCATTCCT TTCTGCCAGT
ATAAATGAGG CCCTGAACAA CAATGGGATC CTGTCAAGTG TTGGAAAGTT CTCTACAGTC
AACCCAGTCT ATGTTGGCAA CGTGGCCTGG GCCCACATTC TGGCCTTGAG GGCTCTGCGG
GACCCCAAGA AGGCCCCAAG TGTCCGAGGT CAATTCTATT ACATCTCAGA TGACACGCCT
CACCAAAGCT ATGATAACCT TAATTACATC CTGAGCAAAG AGTTTGGCCT CCGCCTTGAT
TCCAGATGGA GCCTTCCTTT AACCCTGATG TACTGGATTG GCTTCCTGCT GGAAGTAGTG
AGCTTCCTAC TCAGCCCAAT TTACTCCTAT CAACCCCCCT TCAACCGCCA CACAGTCACA
TTATCAAATA GTGTGTTCAC CTTCTCTTAC AAGAAGGCTC AGCGAGATCT GGCGTATAAG
CCACTCTACA GCTGGGAGGA AGCCAAGCAG AAAACCGTGG AGTGGGTTGG TTCCCTTGTG
GACCGGCACA AGGAGACCCT GAAGTCCAAG ACTCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14034
Accession Version NM_001166120.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1119bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP197992.1, BC038419.1 and AK222997.1. This sequence is a reference standard in the RefSeqGene project. Summary: The protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]. Transcript Variant: This variant (2) contains an alternate 5' non-coding exon, hence differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DA015880.1, DA015880.1, DA432316.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGGCTGGA GCTGCCTTGT GACAGGAGCA GGAGGGCTTC TGGGTCAGAG GATCGTCCGC 
CTGTTGGTGG AAGAGAAGGA ACTGAAGGAG ATCAGGGCCT TGGACAAGGC CTTCAGACCA
GAATTGAGAG AGGAATTTTC TAAGCTCCAG AACAGGACCA AGCTGACTGT ACTTGAAGGA
GACATTCTGG ATGAGCCATT CCTGAAAAGA GCCTGCCAGG ACGTCTCGGT CGTCATCCAC
ACCGCCTGTA TCATTGATGT CTTTGGTGTC ACTCACAGAG AGTCCATCAT GAATGTCAAT
GTGAAAGGTA CCCAGCTACT GTTGGAGGCC TGTGTCCAAG CCAGTGTGCC AGTCTTCATC
TACACCAGTA GCATAGAGGT AGCCGGGCCC AACTCCTACA AGGAAATCAT CCAGAACGGC
CACGAAGAAG AGCCTCTGGA AAACACATGG CCCACTCCAT ACCCGTACAG CAAAAAGCTT
GCTGAGAAGG CTGTGCTGGC GGCTAATGGG TGGAATCTAA AAAATGGTGA TACCTTGTAC
ACTTGTGCGT TAAGACCCAC ATATATCTAT GGGGAAGGAG GCCCATTCCT TTCTGCCAGT
ATAAATGAGG CCCTGAACAA CAATGGGATC CTGTCAAGTG TTGGAAAGTT CTCTACAGTC
AACCCAGTCT ATGTTGGCAA CGTGGCCTGG GCCCACATTC TGGCCTTGAG GGCTCTGCGG
GACCCCAAGA AGGCCCCAAG TGTCCGAGGT CAATTCTATT ACATCTCAGA TGACACGCCT
CACCAAAGCT ATGATAACCT TAATTACATC CTGAGCAAAG AGTTTGGCCT CCGCCTTGAT
TCCAGATGGA GCCTTCCTTT AACCCTGATG TACTGGATTG GCTTCCTGCT GGAAGTAGTG
AGCTTCCTAC TCAGCCCAAT TTACTCCTAT CAACCCCCCT TCAACCGCCA CACAGTCACA
TTATCAAATA GTGTGTTCAC CTTCTCTTAC AAGAAGGCTC AGCGAGATCT GGCGTATAAG
CCACTCTACA GCTGGGAGGA AGCCAAGCAG AAAACCGTGG AGTGGGTTGG TTCCCTTGTG
GACCGGCACA AGGAGACCCT GAAGTCCAAG ACTCAGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001159592.1
CDS250..1368
Misc Feature(1)82..84(+)
Misc Feature(2)259..1317(+)
Misc Feature(3)265..1323(+)
Misc Feature(4)274..816(+)
Misc Feature(5)547..723(+)
Misc Feature(6)619..897(+)
Misc Feature(7)1108..1170(+)
Exon (1)1..160
Gene:HSD3B2
Gene Synonym:
Exon (2)161..391
Gene:HSD3B2
Gene Synonym:
Exon (3)392..556
Gene:HSD3B2
Gene Synonym:
Exon (4)557..1787
Gene:HSD3B2
Gene Synonym:
Translation
Position Chain Variation Link
5 5 c, t dbSNP:188666803
29 29 a, c dbSNP:34282330
80 80 c, t dbSNP:144649719
85 85 a, c dbSNP:587765839
91 91 g, t dbSNP:587638696
104 104 c, t dbSNP:181498954
122 122 c, g dbSNP:773726113
143 143 a, g dbSNP:763262861
156 156 c, t dbSNP:377456276
200 200 g, t dbSNP:781632641
204 204 a, c, t dbSNP:748675518
207 207 c, t dbSNP:778397232
218 218 c, t dbSNP:6208
227 227 c, t dbSNP:771602997
245 245 c, g dbSNP:775250861
248 248 c, t dbSNP:187042846
249 249 a, c, g dbSNP:752085728
250 250 a, t dbSNP:776656223
254 254 g, t dbSNP:116449508
258 258 c, g dbSNP:765335418
260 260 a, g dbSNP:773416276
262 262 a, t dbSNP:376207606
264 264 a, c dbSNP:766474996
270 270 g, t dbSNP:751834454
277 277 g, t dbSNP:755248657
278 278 a, c dbSNP:28934880
282 282 a, g dbSNP:767881300
283 283 a, g dbSNP:753134237
284 284 a, g dbSNP:756607591
285 285 a, g dbSNP:778303029
289 289 c, g dbSNP:377504860
299 299 g, t dbSNP:748554689
300 300 a, g dbSNP:757957605
303 303 c, t dbSNP:777605448
304 304 a, g dbSNP:143169543
305 305 c, t dbSNP:115344376
307 307 c, t dbSNP:139191056
308 308 a, g dbSNP:748018110
309 309 c, g dbSNP:769828729
316 316 a, g dbSNP:773237182
327 327 g, t dbSNP:762899067
337 337 a, g dbSNP:753777808
345 345 a, g dbSNP:771061890
346 346 g, t dbSNP:774377636
351 351 a, g dbSNP:757051767
358 358 a, g dbSNP:759756499
359 359 c, g dbSNP:767793086
363 363 c, t dbSNP:753046362
365 365 c, g dbSNP:761068679
373 373 c, t dbSNP:370732845
375 375 -, ga dbSNP:760846678
376 376 a, g dbSNP:374785266
381 381 g, t dbSNP:111333222
394 394 a, c dbSNP:780947714
396 396 c, g dbSNP:752417564
398 398 a, g dbSNP:755912072
409 409 a, g dbSNP:372260820
414 414 a, g dbSNP:749102084
428 428 a, g dbSNP:141499927
446 446 c, t dbSNP:778763649
451 451 a, c dbSNP:138340684
456 456 a, g dbSNP:375679388
458 458 a, g dbSNP:772162284
462 462 c, t dbSNP:775633331
464 464 a, g, t dbSNP:747237944
469 469 a, g dbSNP:4986954
471 471 c, t dbSNP:150892928
472 472 a, g dbSNP:765748123
474 474 c, g dbSNP:773882717
476 476 c, t dbSNP:759419941
477 477 a, g dbSNP:767147330
480 480 c, t dbSNP:752329512
481 481 a, g dbSNP:143103963
487 487 c, t dbSNP:755849746
489 489 a, c dbSNP:763712349
492 492 c, t dbSNP:753645202
493 493 a, g dbSNP:757033996
495 495 c, t dbSNP:587717286
495 495 c, t dbSNP:778861971
499 499 a, g dbSNP:745820487
515 515 g, t dbSNP:368662017
526 526 a, g dbSNP:371800920
529 529 c, g dbSNP:6211
536 536 c, t dbSNP:767195449
550 550 c, g, t dbSNP:752453060
561 561 c, t dbSNP:367646123
577 577 a, g dbSNP:587730679
577 577 a, g dbSNP:781422494
585 585 c, t dbSNP:748328293
589 589 -, g dbSNP:765602803
592 592 a, g dbSNP:770127588
608 608 c, t dbSNP:778275898
615 615 c, t dbSNP:749726800
618 618 c, t dbSNP:758740855
633 633 c, t dbSNP:775037341
634 634 a, g dbSNP:587628683
634 634 a, g dbSNP:760184076
647 647 a, g dbSNP:768064787
659 659 a, t dbSNP:776281757
660 660 a, c dbSNP:374909270
666 666 c, t dbSNP:369303483
667 667 a, g dbSNP:587759526
667 667 a, g dbSNP:768416823
668 668 g, t dbSNP:142169766
669 669 c, t dbSNP:766267758
672 672 c, t dbSNP:751470493
673 673 a, g, t dbSNP:80358219
676 676 c, g dbSNP:587631086
676 676 c, g dbSNP:781133903
682 682 c, t dbSNP:752886470
685 685 c, t dbSNP:756389486
687 687 a, g dbSNP:587692614
703 703 a, g dbSNP:778188253
709 709 a, c, t dbSNP:749638934
713 713 c, t dbSNP:779418168
714 714 a, c, g dbSNP:114527791
715 715 c, t dbSNP:587676928
715 715 c, t dbSNP:775993749
740 740 c, t dbSNP:34562248
742 742 a, g dbSNP:761437204
749 749 c, t dbSNP:35486059
750 750 a, g dbSNP:6207
754 754 -, aatgggtgg dbSNP:750849889
761 761 a, g dbSNP:80358216
767 767 g, t dbSNP:762479018
774 774 c, t dbSNP:766170141
783 783 c, g dbSNP:751443478
789 789 a, c dbSNP:759383948
798 798 a, g dbSNP:33932258
799 799 c, t dbSNP:752695754
802 802 a, c dbSNP:756301579
804 804 -, c dbSNP:770815049
809 809 c, t dbSNP:764370917
811 811 c, t dbSNP:767943915
817 817 c, t dbSNP:754126326
819 819 c, t dbSNP:374075427
831 831 c, t dbSNP:757629697
842 842 c, t dbSNP:779142287
848 848 a, g dbSNP:377671756
856 856 a, g dbSNP:746303807
858 858 a, g dbSNP:375323828
860 860 c, t dbSNP:758811639
862 862 c, t dbSNP:138811555
864 864 a, g dbSNP:747620720
870 870 c, t dbSNP:143758913
874 874 a, g dbSNP:587669956
874 874 a, g dbSNP:772790725
877 877 a, g dbSNP:587741317
877 877 a, g dbSNP:748940918
879 879 c, t dbSNP:770633737
880 880 c, t dbSNP:143038843
886 886 a, g dbSNP:759422374
887 887 c, g dbSNP:137867568
897 897 g, t dbSNP:775527537
903 903 c, t dbSNP:760787616
906 906 a, t dbSNP:764274511
909 909 c, t dbSNP:754040085
913 913 a, c, g dbSNP:80358220
914 914 a, c dbSNP:765547422
918 918 c, t dbSNP:750725811
920 920 a, g dbSNP:150525350
921 921 c, t dbSNP:116728039
923 923 c, t dbSNP:747532631
926 926 a, g dbSNP:755550493
930 930 c, t dbSNP:587675471
930 930 c, t dbSNP:777257924
931 931 a, g dbSNP:748851282
937 937 c, t dbSNP:770464639
956 956 c, t dbSNP:35887327
963 963 c, t dbSNP:745608432
964 964 c, t dbSNP:771922495
967 967 c, t dbSNP:370603955
968 968 a, g dbSNP:373456025
969 969 a, g dbSNP:200212806
972 972 c, g dbSNP:776831616
973 973 c, t dbSNP:762026737
985 985 c, t dbSNP:587658718
985 985 c, t dbSNP:765340532
988 988 a, g dbSNP:750684493
991 991 aa, gt dbSNP:121964896
991 991 a, g dbSNP:763173486
994 994 c, t dbSNP:80358217
995 995 a, g dbSNP:751984843
999 999 c, t dbSNP:755461071
1011 1011 c, t dbSNP:781698731
1019 1019 a, c dbSNP:587734522
1019 1019 a, c dbSNP:753312765
1025 1025 c, t dbSNP:80358221
1026 1026 a, g dbSNP:33926546
1027 1027 c, t dbSNP:745547471
1038 1038 c, g, t dbSNP:771688849
1057 1057 a, g dbSNP:746851162
1058 1058 c, t dbSNP:75429891
1064 1064 c, g dbSNP:587772926
1064 1064 c, g dbSNP:776743549
1066 1066 -, aa dbSNP:754609778
1067 1067 a, g dbSNP:761754141
1071 1071 c, g dbSNP:769933669
1078 1078 c, t dbSNP:201384931
1081 1081 c, t dbSNP:587711475
1081 1081 c, t dbSNP:763227842
1082 1082 a, g dbSNP:766564863
1094 1094 a, g dbSNP:751897022
1101 1101 a, c, t dbSNP:151258756
1104 1104 a, t dbSNP:753224225
1116 1116 -, g dbSNP:767167623
1124 1124 a, g dbSNP:756663759
1134 1134 c, t dbSNP:778481893
1139 1139 c, t dbSNP:756147663
1140 1140 a, g dbSNP:779139349
1144 1144 a, g dbSNP:758045653
1158 1158 a, c, g dbSNP:757801056
1168 1168 a, g dbSNP:746758931
1173 1173 c, t dbSNP:587775183
1173 1173 c, t dbSNP:768590776
1180 1180 c, t dbSNP:781213951
1182 1182 a, c dbSNP:748116919
1195 1195 c, t dbSNP:114032180
1196 1196 a, g dbSNP:773331795
1200 1200 c, t dbSNP:762995520
1202 1202 c, t dbSNP:771113815
1209 1209 a, c dbSNP:34412477
1212 1212 a, g dbSNP:759846410
1218 1218 c, t dbSNP:767883215
1221 1221 c, t dbSNP:145137295
1224 1224 a, g dbSNP:761230660
1230 1230 a, c dbSNP:149441011
1235 1235 c, t dbSNP:749915716
1239 1239 c, t dbSNP:757875458
1241 1241 a, t dbSNP:766077589
1252 1252 a, c, t dbSNP:148200568
1256 1256 a, t dbSNP:781125519
1261 1261 a, g dbSNP:147383167
1262 1262 c, g, t dbSNP:756060240
1263 1263 a, g, t dbSNP:116342586
1271 1271 c, t dbSNP:121964897
1274 1274 g, t dbSNP:774500247
1275 1275 c, g dbSNP:746095411
1278 1278 c, t dbSNP:35849160
1281 1281 c, t dbSNP:772348263
1287 1287 a, g dbSNP:775901269
1293 1293 c, t dbSNP:760983984
1299 1299 a, g dbSNP:764620496
1304 1304 a, c dbSNP:777023732
1305 1305 c, g, t dbSNP:781770416
1306 1306 a, g dbSNP:143634775
1311 1311 a, g dbSNP:114064941
1313 1313 a, g dbSNP:767128094
1329 1329 g, t dbSNP:752508551
1333 1333 c, t dbSNP:199919404
1334 1334 a, g dbSNP:777718760
1346 1346 a, c dbSNP:749198869
1347 1347 c, t dbSNP:757261352
1362 1362 a, t dbSNP:778961069
1368 1368 a, c, g dbSNP:80358218
1372 1372 -, aggatgac dbSNP:752135021
1375 1375 a, g dbSNP:772250386
1380 1380 c, g dbSNP:775605483
1382 1382 a, g dbSNP:747348624
1387 1387 a, g dbSNP:768913118
1388 1388 g, t dbSNP:777126692
1389 1389 c, g dbSNP:762402613
1391 1391 a, g dbSNP:770477643
1396 1396 c, g dbSNP:587718678
1396 1396 c, g dbSNP:773980654
1400 1400 c, g, t dbSNP:759161114
1402 1402 g, t dbSNP:752317712
1403 1403 g, t dbSNP:760462265
1410 1410 a, c dbSNP:763802578
1413 1413 c, t dbSNP:753700611
1414 1414 c, t dbSNP:371081728
1415 1415 a, t dbSNP:374222089
1430 1430 c, t dbSNP:587599964
1464 1464 c, g dbSNP:9282703
1570 1570 a, g dbSNP:9282704
1575 1575 c, t dbSNP:186430448
1595 1595 g, t dbSNP:587653430
1637 1637 g, t dbSNP:191329987
1644 1644 cttaac, t dbSNP:386635104
1644 1644 c, t dbSNP:1819698
1647 1647 -, aactt dbSNP:369148049
1650 1650 c, t dbSNP:587679988
1673 1673 c, g dbSNP:587758091
1683 1683 -, c dbSNP:754135008
1689 1689 c, g dbSNP:1361530
1721 1721 c, t dbSNP:587707312
1722 1722 c, t dbSNP:747877605
1735 1735 a, g dbSNP:769352247
1765 1765 a, t dbSNP:753468192

Target ORF information:

RefSeq Version NM_001166120
Organism Homo sapiens (human)
Definition Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (HSD3B2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001166120

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGGCTGGA GCTGCCTTGT GACAGGAGCA GGAGGGCTTC TGGGTCAGAG GATCGTCCGC 
CTGTTGGTGG AAGAGAAGGA ACTGAAGGAG ATCAGGGCCT TGGACAAGGC CTTCAGACCA
GAATTGAGAG AGGAATTTTC TAAGCTCCAG AACAGGACCA AGCTGACTGT ACTTGAAGGA
GACATTCTGG ATGAGCCATT CCTGAAAAGA GCCTGCCAGG ACGTCTCGGT CGTCATCCAC
ACCGCCTGTA TCATTGATGT CTTTGGTGTC ACTCACAGAG AGTCCATCAT GAATGTCAAT
GTGAAAGGTA CCCAGCTACT GTTGGAGGCC TGTGTCCAAG CCAGTGTGCC AGTCTTCATC
TACACCAGTA GCATAGAGGT AGCCGGGCCC AACTCCTACA AGGAAATCAT CCAGAACGGC
CACGAAGAAG AGCCTCTGGA AAACACATGG CCCACTCCAT ACCCGTACAG CAAAAAGCTT
GCTGAGAAGG CTGTGCTGGC GGCTAATGGG TGGAATCTAA AAAATGGTGA TACCTTGTAC
ACTTGTGCGT TAAGACCCAC ATATATCTAT GGGGAAGGAG GCCCATTCCT TTCTGCCAGT
ATAAATGAGG CCCTGAACAA CAATGGGATC CTGTCAAGTG TTGGAAAGTT CTCTACAGTC
AACCCAGTCT ATGTTGGCAA CGTGGCCTGG GCCCACATTC TGGCCTTGAG GGCTCTGCGG
GACCCCAAGA AGGCCCCAAG TGTCCGAGGT CAATTCTATT ACATCTCAGA TGACACGCCT
CACCAAAGCT ATGATAACCT TAATTACATC CTGAGCAAAG AGTTTGGCCT CCGCCTTGAT
TCCAGATGGA GCCTTCCTTT AACCCTGATG TACTGGATTG GCTTCCTGCT GGAAGTAGTG
AGCTTCCTAC TCAGCCCAAT TTACTCCTAT CAACCCCCCT TCAACCGCCA CACAGTCACA
TTATCAAATA GTGTGTTCAC CTTCTCTTAC AAGAAGGCTC AGCGAGATCT GGCGTATAAG
CCACTCTACA GCTGGGAGGA AGCCAAGCAG AAAACCGTGG AGTGGGTTGG TTCCCTTGTG
GACCGGCACA AGGAGACCCT GAAGTCCAAG ACTCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.