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HSD3B2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol HSD3B2
Entrez Gene ID 3284
Full Name hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
Synonyms HSD3B, HSDB, SDR11E2
General protein information
Preferred Names
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Names
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
3-beta-HSD II
3 beta-HSD type II
progesterone reductase
delta 5-delta 4-isomerase type II
3-beta-HSD adrenal and gonadal type
3-beta-hydroxy-5-ene steroid dehydrogenase
3-beta-hydroxy-Delta(5)-steroid dehydrogenase
short chain dehydrogenase/reductase family 11E, member 2
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II
3 beta-hydroxysteroid dehydrogenase type II, delta 5-delta 4-isomerase type II, 3 beta-HSD type II
NP_000189.1
NP_001159592.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1p13.1

Summary The protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]. lac of sum
Disorder MIM:

613890


mRNA and Protein(s)

mRNA Protein Name
NM_000198 NP_000189 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
NM_001166120 NP_001159592 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2



Homo sapiens (human) HSD3B2 NP_001159592.1
Pan troglodytes (chimpanzee) HSD3B2 XP_001142555.1
Macaca mulatta (Rhesus monkey) LOC100424395 XP_002801771.1
Canis lupus familiaris (dog) HSD3B2 NP_001010954.1
Bos taurus (cattle) HSD3B1 NP_776768.1
Mus musculus (house mouse) Hsd3b1 NP_032319.1
Rattus norvegicus (Norway rat) Hsd3b NP_001036084.1
Rattus norvegicus (Norway rat) Hsd3b6 NP_058961.4
Rattus norvegicus (Norway rat) LOC100911154 XP_006233083.1
Gallus gallus (chicken) HSD3B2 NP_990449.1
Danio rerio (zebrafish) si:rp71-68n21.10 XP_694204.3
Danio rerio (zebrafish) hsd3b1 NP_997962.1
Drosophila melanogaster (fruit fly) CG7724 NP_648957.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC100490622 XP_004920800.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HSD3B2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSD3B2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu14034
NM_000198 Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (HSD3B2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu14034
NM_001166120 Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (HSD3B2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu14034
Clone ID Related Accession (Same CDS sequence) NM_000198 , NM_001166120
Accession Version NM_000198.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1119bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AV705363.1, BC038419.1 and AK222997.1. On Oct 8, 2009 this sequence version replaced gi:50726973. Transcript Variant: This variant (1) represents the predominant transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222997.1, BC038419.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGGCTGGA GCTGCCTTGT GACAGGAGCA GGAGGGCTTC TGGGTCAGAG GATCGTCCGC 
CTGTTGGTGG AAGAGAAGGA ACTGAAGGAG ATCAGGGCCT TGGACAAGGC CTTCAGACCA
GAATTGAGAG AGGAATTTTC TAAGCTCCAG AACAGGACCA AGCTGACTGT ACTTGAAGGA
GACATTCTGG ATGAGCCATT CCTGAAAAGA GCCTGCCAGG ACGTCTCGGT CGTCATCCAC
ACCGCCTGTA TCATTGATGT CTTTGGTGTC ACTCACAGAG AGTCCATCAT GAATGTCAAT
GTGAAAGGTA CCCAGCTACT GTTGGAGGCC TGTGTCCAAG CCAGTGTGCC AGTCTTCATC
TACACCAGTA GCATAGAGGT AGCCGGGCCC AACTCCTACA AGGAAATCAT CCAGAACGGC
CACGAAGAAG AGCCTCTGGA AAACACATGG CCCACTCCAT ACCCGTACAG CAAAAAGCTT
GCTGAGAAGG CTGTGCTGGC GGCTAATGGG TGGAATCTAA AAAATGGTGA TACCTTGTAC
ACTTGTGCGT TAAGACCCAC ATATATCTAT GGGGAAGGAG GCCCATTCCT TTCTGCCAGT
ATAAATGAGG CCCTGAACAA CAATGGGATC CTGTCAAGTG TTGGAAAGTT CTCTACAGTC
AACCCAGTCT ATGTTGGCAA CGTGGCCTGG GCCCACATTC TGGCCTTGAG GGCTCTGCGG
GACCCCAAGA AGGCCCCAAG TGTCCGAGGT CAATTCTATT ACATCTCAGA TGACACGCCT
CACCAAAGCT ATGATAACCT TAATTACATC CTGAGCAAAG AGTTTGGCCT CCGCCTTGAT
TCCAGATGGA GCCTTCCTTT AACCCTGATG TACTGGATTG GCTTCCTGCT GGAAGTAGTG
AGCTTCCTAC TCAGCCCAAT TTACTCCTAT CAACCCCCCT TCAACCGCCA CACAGTCACA
TTATCAAATA GTGTGTTCAC CTTCTCTTAC AAGAAGGCTC AGCGAGATCT GGCGTATAAG
CCACTCTACA GCTGGGAGGA AGCCAAGCAG AAAACCGTGG AGTGGGTTGG TTCCCTTGTG
GACCGGCACA AGGAGACCCT GAAGTCCAAG ACTCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000189.1
CDS173..1291
Misc Feature(1)38..40(+)
Misc Feature(2)182..1240(+)
Misc Feature(3)188..1246(+)
Misc Feature(4)197..739(+)
Misc Feature(5)470..646(+)
Misc Feature(6)542..820(+)
Misc Feature(7)1031..1093(+)
Exon (1)1..83
Gene:HSD3B2
Gene Synonym:
Exon (2)84..314
Gene:HSD3B2
Gene Synonym:
Exon (3)315..479
Gene:HSD3B2
Gene Synonym:
Exon (4)480..1710
Gene:HSD3B2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000198
Organism Homo sapiens (human)
Definition Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (HSD3B2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000198

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGGCTGGA GCTGCCTTGT GACAGGAGCA GGAGGGCTTC TGGGTCAGAG GATCGTCCGC 
CTGTTGGTGG AAGAGAAGGA ACTGAAGGAG ATCAGGGCCT TGGACAAGGC CTTCAGACCA
GAATTGAGAG AGGAATTTTC TAAGCTCCAG AACAGGACCA AGCTGACTGT ACTTGAAGGA
GACATTCTGG ATGAGCCATT CCTGAAAAGA GCCTGCCAGG ACGTCTCGGT CGTCATCCAC
ACCGCCTGTA TCATTGATGT CTTTGGTGTC ACTCACAGAG AGTCCATCAT GAATGTCAAT
GTGAAAGGTA CCCAGCTACT GTTGGAGGCC TGTGTCCAAG CCAGTGTGCC AGTCTTCATC
TACACCAGTA GCATAGAGGT AGCCGGGCCC AACTCCTACA AGGAAATCAT CCAGAACGGC
CACGAAGAAG AGCCTCTGGA AAACACATGG CCCACTCCAT ACCCGTACAG CAAAAAGCTT
GCTGAGAAGG CTGTGCTGGC GGCTAATGGG TGGAATCTAA AAAATGGTGA TACCTTGTAC
ACTTGTGCGT TAAGACCCAC ATATATCTAT GGGGAAGGAG GCCCATTCCT TTCTGCCAGT
ATAAATGAGG CCCTGAACAA CAATGGGATC CTGTCAAGTG TTGGAAAGTT CTCTACAGTC
AACCCAGTCT ATGTTGGCAA CGTGGCCTGG GCCCACATTC TGGCCTTGAG GGCTCTGCGG
GACCCCAAGA AGGCCCCAAG TGTCCGAGGT CAATTCTATT ACATCTCAGA TGACACGCCT
CACCAAAGCT ATGATAACCT TAATTACATC CTGAGCAAAG AGTTTGGCCT CCGCCTTGAT
TCCAGATGGA GCCTTCCTTT AACCCTGATG TACTGGATTG GCTTCCTGCT GGAAGTAGTG
AGCTTCCTAC TCAGCCCAAT TTACTCCTAT CAACCCCCCT TCAACCGCCA CACAGTCACA
TTATCAAATA GTGTGTTCAC CTTCTCTTAC AAGAAGGCTC AGCGAGATCT GGCGTATAAG
CCACTCTACA GCTGGGAGGA AGCCAAGCAG AAAACCGTGG AGTGGGTTGG TTCCCTTGTG
GACCGGCACA AGGAGACCCT GAAGTCCAAG ACTCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14034
Clone ID Related Accession (Same CDS sequence) NM_000198 , NM_001166120
Accession Version NM_001166120.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1119bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP197992.1, BC038419.1 and AK222997.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) contains an alternate 5' non-coding exon, hence differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DA015880.1, DA015880.1, DA432316.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGGCTGGA GCTGCCTTGT GACAGGAGCA GGAGGGCTTC TGGGTCAGAG GATCGTCCGC 
CTGTTGGTGG AAGAGAAGGA ACTGAAGGAG ATCAGGGCCT TGGACAAGGC CTTCAGACCA
GAATTGAGAG AGGAATTTTC TAAGCTCCAG AACAGGACCA AGCTGACTGT ACTTGAAGGA
GACATTCTGG ATGAGCCATT CCTGAAAAGA GCCTGCCAGG ACGTCTCGGT CGTCATCCAC
ACCGCCTGTA TCATTGATGT CTTTGGTGTC ACTCACAGAG AGTCCATCAT GAATGTCAAT
GTGAAAGGTA CCCAGCTACT GTTGGAGGCC TGTGTCCAAG CCAGTGTGCC AGTCTTCATC
TACACCAGTA GCATAGAGGT AGCCGGGCCC AACTCCTACA AGGAAATCAT CCAGAACGGC
CACGAAGAAG AGCCTCTGGA AAACACATGG CCCACTCCAT ACCCGTACAG CAAAAAGCTT
GCTGAGAAGG CTGTGCTGGC GGCTAATGGG TGGAATCTAA AAAATGGTGA TACCTTGTAC
ACTTGTGCGT TAAGACCCAC ATATATCTAT GGGGAAGGAG GCCCATTCCT TTCTGCCAGT
ATAAATGAGG CCCTGAACAA CAATGGGATC CTGTCAAGTG TTGGAAAGTT CTCTACAGTC
AACCCAGTCT ATGTTGGCAA CGTGGCCTGG GCCCACATTC TGGCCTTGAG GGCTCTGCGG
GACCCCAAGA AGGCCCCAAG TGTCCGAGGT CAATTCTATT ACATCTCAGA TGACACGCCT
CACCAAAGCT ATGATAACCT TAATTACATC CTGAGCAAAG AGTTTGGCCT CCGCCTTGAT
TCCAGATGGA GCCTTCCTTT AACCCTGATG TACTGGATTG GCTTCCTGCT GGAAGTAGTG
AGCTTCCTAC TCAGCCCAAT TTACTCCTAT CAACCCCCCT TCAACCGCCA CACAGTCACA
TTATCAAATA GTGTGTTCAC CTTCTCTTAC AAGAAGGCTC AGCGAGATCT GGCGTATAAG
CCACTCTACA GCTGGGAGGA AGCCAAGCAG AAAACCGTGG AGTGGGTTGG TTCCCTTGTG
GACCGGCACA AGGAGACCCT GAAGTCCAAG ACTCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001159592.1
CDS250..1368
Misc Feature(1)82..84(+)
Misc Feature(2)259..1317(+)
Misc Feature(3)265..1323(+)
Misc Feature(4)274..816(+)
Misc Feature(5)547..723(+)
Misc Feature(6)619..897(+)
Misc Feature(7)1108..1170(+)
Exon (1)1..160
Gene:HSD3B2
Gene Synonym:
Exon (2)161..391
Gene:HSD3B2
Gene Synonym:
Exon (3)392..556
Gene:HSD3B2
Gene Synonym:
Exon (4)557..1787
Gene:HSD3B2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001166120
Organism Homo sapiens (human)
Definition Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (HSD3B2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001166120

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGGCTGGA GCTGCCTTGT GACAGGAGCA GGAGGGCTTC TGGGTCAGAG GATCGTCCGC 
CTGTTGGTGG AAGAGAAGGA ACTGAAGGAG ATCAGGGCCT TGGACAAGGC CTTCAGACCA
GAATTGAGAG AGGAATTTTC TAAGCTCCAG AACAGGACCA AGCTGACTGT ACTTGAAGGA
GACATTCTGG ATGAGCCATT CCTGAAAAGA GCCTGCCAGG ACGTCTCGGT CGTCATCCAC
ACCGCCTGTA TCATTGATGT CTTTGGTGTC ACTCACAGAG AGTCCATCAT GAATGTCAAT
GTGAAAGGTA CCCAGCTACT GTTGGAGGCC TGTGTCCAAG CCAGTGTGCC AGTCTTCATC
TACACCAGTA GCATAGAGGT AGCCGGGCCC AACTCCTACA AGGAAATCAT CCAGAACGGC
CACGAAGAAG AGCCTCTGGA AAACACATGG CCCACTCCAT ACCCGTACAG CAAAAAGCTT
GCTGAGAAGG CTGTGCTGGC GGCTAATGGG TGGAATCTAA AAAATGGTGA TACCTTGTAC
ACTTGTGCGT TAAGACCCAC ATATATCTAT GGGGAAGGAG GCCCATTCCT TTCTGCCAGT
ATAAATGAGG CCCTGAACAA CAATGGGATC CTGTCAAGTG TTGGAAAGTT CTCTACAGTC
AACCCAGTCT ATGTTGGCAA CGTGGCCTGG GCCCACATTC TGGCCTTGAG GGCTCTGCGG
GACCCCAAGA AGGCCCCAAG TGTCCGAGGT CAATTCTATT ACATCTCAGA TGACACGCCT
CACCAAAGCT ATGATAACCT TAATTACATC CTGAGCAAAG AGTTTGGCCT CCGCCTTGAT
TCCAGATGGA GCCTTCCTTT AACCCTGATG TACTGGATTG GCTTCCTGCT GGAAGTAGTG
AGCTTCCTAC TCAGCCCAAT TTACTCCTAT CAACCCCCCT TCAACCGCCA CACAGTCACA
TTATCAAATA GTGTGTTCAC CTTCTCTTAC AAGAAGGCTC AGCGAGATCT GGCGTATAAG
CCACTCTACA GCTGGGAGGA AGCCAAGCAG AAAACCGTGG AGTGGGTTGG TTCCCTTGTG
GACCGGCACA AGGAGACCCT GAAGTCCAAG ACTCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Association of the variations in the HSD3beta gene with primary aldosteronism
J. Hypertens. 31 (7), 1396-1405 (2013)
Wu VC, Wu CK, Chang YC, Young GH, Chen SC, Yang WS, Chen CY, Wang WJ, Lin CY, Lin YH, Lin SL, Chueh SC and Wu KD.


book

Interleukin-4 and prostaglandin E2 synergistically up-regulate 3beta-hydroxysteroid dehydrogenase type 2 in endometrioma stromal cells
J. Clin. Endocrinol. Metab. 98 (4), 1583-1590 (2013)
Urata Y, Osuga Y, Akiyama I, Nagai M, Izumi G, Takamura M, Hasegawa A, Harada M, Hirata T, Hirota Y, Yoshino O, Koga K and Kozuma S.


book

Characterization of steroidogenic enzyme expression in aldosterone-producing adenoma: a comparison with various human adrenal tumors
Endocr. J. 60 (3), 329-336 (2013)
Sakuma I, Suematsu S, Matsuzawa Y, Saito J, Omura M, Maekawa T, Nakamura Y, Sasano H and Nishikawa T.


book

Mutation of 3beta-hydroxysteroid dehydrogenase (3beta-HSD) at the 3'-untranslated region is associated with adrenocortical insufficiency
Mol Med Rep 6 (6), 1305-1308 (2012)
Pan Y, Zhong S, Hu RM and Gong W.


book

HSD3B and gene-gene interactions in a pathway-based analysis of genetic susceptibility to bladder cancer
PLoS ONE 7 (12), E51301 (2012)
Andrew AS, Hu T, Gu J, Gui J, Ye Y, Marsit CJ, Kelsey KT, Schned AR, Tanyos SA, Pendleton EM, Mason RA, Morlock EV, Zens MS, Li Z, Moore JH, Wu X and Karagas MR.


book

Congenital adrenal hyperplasia due to point mutations in the type II 3 beta-hydroxysteroid dehydrogenase gene
Nat. Genet. 1 (4), 239-245 (1992)
Rheaume E, Simard J, Morel Y, Mebarki F, Zachmann M, Forest MG, New MI and Labrie F.


book

Structure of the human type II 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase (3 beta-HSD) gene: adrenal and gonadal specificity
DNA Cell Biol. 10 (10), 701-711 (1991)
Lachance Y, Luu-The V, Verreault H, Dumont M, Rheaume E, Leblanc G and Labrie F.


book

Structure and expression of a new complementary DNA encoding the almost exclusive 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase in human adrenals and gonads
Mol. Endocrinol. 5 (8), 1147-1157 (1991)
Rheaume E, Lachance Y, Zhao HF, Breton N, Dumont M, de Launoit Y, Trudel C, Luu-The V, Simard J and Labrie F.


book

Regional chromosomal assignment of human 3-beta-hydroxy-5-ene steroid dehydrogenase to 1p13.1 by non-isotopic in situ hybridisation
Hum. Genet. 87 (2), 223-225 (1991)
Morrison N, Nickson DA, McBride MW, Mueller UW, Boyd E and Sutcliffe RG.


book

3 beta-hydroxysteroid dehydrogenase deficiency. Follow-up study in a girl with pubertal bone age
Horm. Res. 11 (6), 292-302 (1979)
Zachmann,M., Forest,M.G. and De Peretti,E.


 
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