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HSPA8 heat shock 70kDa protein 8 [Homo sapiens (human)]

Gene Symbol HSPA8
Entrez Gene ID 3312
Full Name heat shock 70kDa protein 8
Synonyms HEL-33, HEL-S-72p, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP-1, LAP1, NIP71
General protein information
Preferred Names
heat shock cognate 71 kDa protein
Names
heat shock cognate 71 kDa protein
LPS-associated protein 1
heat shock 70kd protein 10
heat shock cognate protein 54
constitutive heat shock protein 70
lipopolysaccharide-associated protein 1
N-myristoyltransferase inhibitor protein 71
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11q24.1

Summary This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]. lac of sum
Disorder MIM:

600816

Disorder Html:
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu27538 XM_011542798 PREDICTED: Homo sapiens heat shock 70kDa protein 8 (HSPA8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu27538 NM_006597 Homo sapiens heat shock 70kDa protein 8 (HSPA8), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu27531 NM_153201 Homo sapiens heat shock 70kDa protein 8 (HSPA8), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu27538D
Sequence Information ORF Nucleotide Sequence (Length: 1941bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product heat shock cognate 71 kDa protein isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_033899.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA
CTCACAGGCA TACCTCCTGC ACCCCGAGGT GTTCCTCAGA TTGAAGTCAC TTTTGACATT
GATGCCAATG GTATACTCAA TGTCTCTGCT GTGGACAAGA GTACGGGAAA AGAGAACAAG
ATTACTATCA CTAATGACAA GGGCCGTTTG AGCAAGGAAG ACATTGAACG TATGGTCCAG
GAAGCTGAGA AGTACAAAGC TGAAGATGAG AAGCAGAGGG ACAAGGTGTC ATCCAAGAAT
TCACTTGAGT CCTATGCCTT CAACATGAAA GCAACTGTTG AAGATGAGAA ACTTCAAGGC
AAGATTAACG ATGAGGACAA ACAGAAGATT CTGGACAAGT GTAATGAAAT TATCAACTGG
CTTGATAAGA ATCAGACTGC TGAGAAGGAA GAATTTGAAC ATCAACAGAA AGAGCTGGAG
AAAGTTTGCA ACCCCATCAT CACCAAGCTG TACCAGAGTG CAGGAGGCAT GCCAGGAGGA
ATGCCTGGGG GATTTCCTGG TGGTGGAGCT CCTCCCTCTG GTGGTGCTTC CTCAGGGCCC
ACCATTGAAG AGGTTGATTTA A
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011541100.1
CDS26..1966
Misc Feature(1)26..1864(+)
Misc Feature(2)41..1168(+)
Misc Feature(3)53..1123(+)
Misc Feature(4)92..1051(+)
Misc Feature(5)122..907(+)
Misc Feature(6)479..1000(+)
Translation
Position Chain Variation Link
22 22 a, t dbSNP:745900938
25 25 c, t dbSNP:774277451
34 34 a, g dbSNP:771060966
39 39 c, t dbSNP:749485084
41 41 g, t dbSNP:778022948
49 49 c, t dbSNP:770097995
61 61 c, t dbSNP:536070549
67 67 c, t dbSNP:144123192
94 94 c, t dbSNP:755455419
97 97 a, g dbSNP:752166960
103 103 c, g dbSNP:779708153
119 119 g, t dbSNP:11551602
136 136 c, t dbSNP:758030256
145 145 c, t dbSNP:750047710
146 146 c, t dbSNP:75739900
147 147 a, g dbSNP:765012279
151 151 c, g, t dbSNP:369851462
154 154 c, g, t dbSNP:760522151
159 159 c, g, t dbSNP:201477863
160 160 a, g dbSNP:775162274
164 164 a, g dbSNP:770980780
170 170 a, c dbSNP:763047269
178 178 a, c, t dbSNP:376156706
187 187 c, g, t dbSNP:767193605
190 190 a, c dbSNP:781396927
220 220 c, t dbSNP:769131425
238 238 a, g dbSNP:776675548
242 242 c, g dbSNP:764381465
244 244 g, t dbSNP:761062129
253 253 a, c dbSNP:775980264
277 277 a, g dbSNP:570819499
298 298 c, g dbSNP:746344123
301 301 c, t dbSNP:775043697
307 307 a, g dbSNP:770300995
310 310 g, t dbSNP:748864676
312 312 a, g dbSNP:777228127
313 313 c, t dbSNP:11551601
319 319 c, t dbSNP:755808417
322 322 c, t dbSNP:201223120
331 331 a, g dbSNP:780961303
334 334 c, g, t dbSNP:751477695
336 336 a, g dbSNP:766369333
341 341 c, g dbSNP:200601870
345 345 a, g dbSNP:748008431
346 346 c, t dbSNP:371835507
351 351 a, g dbSNP:781044959
352 352 a, c dbSNP:764136878
355 355 a, g dbSNP:760971468
358 358 c, g, t dbSNP:140536048
371 371 a, c dbSNP:11551608
373 373 a, g dbSNP:759998595
395 395 c, t dbSNP:774683371
400 400 a, g dbSNP:147610984
403 403 a, g dbSNP:374961418
409 409 a, g dbSNP:772769841
418 418 a, g dbSNP:769361497
419 419 c, g dbSNP:747769427
420 420 a, g dbSNP:780873363
427 427 c, t dbSNP:768398499
438 438 c, g dbSNP:774366889
442 442 c, t dbSNP:771009079
443 443 -, a dbSNP:777175548
444 444 a, c, g dbSNP:141156763
447 447 a, g dbSNP:569651128
450 450 c, t dbSNP:755052827
478 478 c, t dbSNP:747234098
498 498 c, t dbSNP:11551606
511 511 a, g dbSNP:145779755
520 520 a, c, t dbSNP:141917932
529 529 c, t dbSNP:765647043
547 547 c, t dbSNP:757755949
574 574 c, t dbSNP:148609753
591 591 g, t dbSNP:199619043
592 592 g, t dbSNP:200486073
595 595 a, g dbSNP:773246178
596 596 a, g dbSNP:769624839
597 597 c, g, t dbSNP:775427523
598 598 a, g dbSNP:772233016
607 607 a, c, t dbSNP:779126635
613 613 a, c, t dbSNP:749654902
619 619 c, t dbSNP:573467766
622 622 c, t dbSNP:376403473
650 650 a, g dbSNP:778283472
658 658 c, t dbSNP:756568266
682 682 a, c dbSNP:753317621
689 689 a, g dbSNP:11551599
730 730 c, t dbSNP:201712472
733 733 a, g dbSNP:373526651
741 741 a, g dbSNP:536669943
742 742 c, t dbSNP:762772078
748 748 c, t dbSNP:750169512
752 752 g, t dbSNP:765204439
754 754 a, c, t dbSNP:761855165
760 760 c, t dbSNP:775590979
766 766 c, g dbSNP:761623666
775 775 a, g dbSNP:776678845
781 781 c, t dbSNP:369763812
782 782 a, g dbSNP:759639896
784 784 a, c, t dbSNP:150964023
787 787 c, t dbSNP:772077059
799 799 a, g dbSNP:749572958
808 808 a, g dbSNP:777998627
812 812 c, t dbSNP:770238029
814 814 c, t dbSNP:759775156
815 815 c, t dbSNP:748585744
817 817 c, t dbSNP:143480238
830 830 c, t dbSNP:11551604
839 839 c, t dbSNP:11551605
844 844 c, g, t dbSNP:774484203
845 845 c, g dbSNP:751121248
847 847 c, t dbSNP:779796438
853 853 c, t dbSNP:758117987
870 870 c, t dbSNP:750129947
877 877 c, t dbSNP:148846437
886 886 c, g, t dbSNP:551131709
889 889 c, t dbSNP:764020103
896 896 a, g dbSNP:532778326
898 898 c, t dbSNP:538018718
901 901 c, t dbSNP:766515357
909 909 c, t dbSNP:763133730
912 912 c, t dbSNP:773536032
919 919 c, t dbSNP:200035932
925 925 c, t dbSNP:749718428
931 931 c, t dbSNP:748576941
938 938 c, g, t dbSNP:778115074
940 940 c, g dbSNP:145580523
945 945 c, t dbSNP:747578888
946 946 c, t dbSNP:143373443
965 965 c, t dbSNP:758028103
973 973 a, g dbSNP:745458075
1003 1003 a, g dbSNP:770224594
1006 1006 c, t dbSNP:376828215
1009 1009 a, g dbSNP:753679532
1012 1012 a, g dbSNP:139992369
1014 1014 a, t dbSNP:373235233
1016 1016 a, g dbSNP:752672546
1018 1018 g, t dbSNP:766425028
1026 1026 c, t dbSNP:763116637
1027 1027 c, t dbSNP:146503730
1030 1030 c, t dbSNP:115882568
1070 1070 c, t dbSNP:762028517
1075 1075 c, g dbSNP:777124482
1081 1081 c, t dbSNP:139484659
1083 1083 a, t dbSNP:747492962
1084 1084 c, t dbSNP:150854686
1087 1087 c, t dbSNP:771663386
1090 1090 c, t dbSNP:529656968
1099 1099 a, g dbSNP:142431740
1104 1104 a, g dbSNP:756920138
1111 1111 c, t dbSNP:748958068
1117 1117 c, t dbSNP:148737498
1129 1129 g, t dbSNP:577357118
1136 1136 -, tt dbSNP:771981132
1146 1146 c, t dbSNP:746781077
1150 1150 a, c dbSNP:775435579
1165 1165 a, g dbSNP:772121271
1174 1174 c, t dbSNP:531182299
1183 1183 a, g dbSNP:745866854
1199 1199 c, t dbSNP:142113865
1201 1201 a, g dbSNP:756309650
1204 1204 c, t dbSNP:748355748
1216 1216 c, t dbSNP:781302641
1220 1220 c, t dbSNP:755304850
1225 1225 c, t dbSNP:144361240
1250 1250 a, g dbSNP:372928718
1261 1261 a, c, t dbSNP:371408292
1279 1279 c, t dbSNP:550170308
1282 1282 c, t dbSNP:368207632
1285 1285 c, t dbSNP:761340541
1294 1294 a, g dbSNP:139016926
1305 1305 c, t dbSNP:763663403
1315 1315 c, g dbSNP:760433396
1324 1324 c, t dbSNP:775143184
1326 1326 a, g dbSNP:772031159
1330 1330 a, g dbSNP:759523701
1333 1333 c, t dbSNP:757222670
1336 1336 g, t dbSNP:775451393
1339 1339 a, g dbSNP:774454573
1360 1360 c, t dbSNP:767284605
1375 1375 a, g dbSNP:553450668
1387 1387 c, t dbSNP:759347844
1390 1390 c, g dbSNP:751387334
1393 1393 a, t dbSNP:377257990
1400 1400 a, g dbSNP:11551598
1409 1409 a, g dbSNP:746441724
1422 1422 c, t dbSNP:773253340
1423 1423 g, t dbSNP:764034057
1450 1450 a, g dbSNP:760810506
1459 1459 c, t dbSNP:775629110
1465 1465 c, t dbSNP:772264576
1474 1474 c, t dbSNP:199504970
1480 1480 a, c dbSNP:1064585
1483 1483 c, t dbSNP:771376397
1485 1485 a, g dbSNP:749707173
1489 1489 c, g dbSNP:571307052
1492 1492 a, t dbSNP:778373831
1504 1504 a, g dbSNP:138544510
1510 1510 a, g dbSNP:147529697
1524 1524 a, g dbSNP:780908558
1531 1531 c, g, t dbSNP:111774985
1536 1536 c, g dbSNP:766069802
1546 1546 a, g dbSNP:758237764
1575 1575 a, g, t dbSNP:771746646
1576 1576 g, t dbSNP:536065122
1580 1580 a, g dbSNP:778743630
1588 1588 a, g dbSNP:143434494
1595 1595 a, g dbSNP:753733140
1597 1597 a, g dbSNP:371945853
1615 1615 a, g dbSNP:137943836
1622 1622 a, c dbSNP:751646265
1623 1623 a, g dbSNP:766437819
1626 1626 a, c dbSNP:763204793
1633 1633 a, g dbSNP:773460738
1639 1639 a, c, g, t dbSNP:777216036
1653 1653 -, agtc dbSNP:751625114
1658 1658 -, ttc dbSNP:766511510
1659 1659 a, g dbSNP:769269163
1661 1661 g, t dbSNP:761149194
1662 1662 -, cc dbSNP:763022802
1666 1666 c, g dbSNP:775032110
1668 1668 -, gg dbSNP:773074126
1670 1670 a, g dbSNP:568778183
1672 1672 -, gaa dbSNP:769802670
1674 1674 a, g dbSNP:745475251
1676 1676 -, gact dbSNP:761484393
1679 1679 a, g dbSNP:778459169
1682 1682 a, g dbSNP:770645829
1687 1687 -, agatga dbSNP:776539014
1700 1700 c, g dbSNP:749093478
1709 1709 a, g dbSNP:777603150
1713 1713 a, g dbSNP:756041393
1714 1714 a, c, t dbSNP:199882006
1715 1715 a, g dbSNP:765561454
1717 1717 c, t dbSNP:113013616
1732 1732 a, g dbSNP:750621530
1738 1738 a, g dbSNP:765564967
1744 1744 a, g dbSNP:757641811
1746 1746 c, g dbSNP:754246861
1757 1757 a, g dbSNP:764589322
1762 1762 c, t dbSNP:761220214
1774 1774 a, g dbSNP:775881301
1786 1786 c, g, t dbSNP:4802
1798 1798 a, g dbSNP:761588256
1804 1804 a, g dbSNP:112636139
1807 1807 c, t dbSNP:41487746
1808 1808 a, c dbSNP:776255814
1810 1810 a, g dbSNP:768540563
1811 1811 a, c dbSNP:775363477
1817 1817 a, g dbSNP:760638348
1820 1820 c, t dbSNP:145240324
1837 1837 c, t dbSNP:370796977
1839 1839 c, t dbSNP:377681653
1840 1840 a, c, t dbSNP:118171965
1849 1849 c, t dbSNP:188408273
1854 1854 g, t dbSNP:781388678
1858 1858 c, t dbSNP:755360656
1866 1866 c, t dbSNP:752022565
1871 1871 a, g dbSNP:780546314
1884 1884 a, g dbSNP:757849635
1900 1900 c, t dbSNP:780073872
1906 1906 c, t dbSNP:201479266
1907 1907 a, g dbSNP:764872570
1912 1912 a, g dbSNP:758520548
1914 1914 c, t dbSNP:753546825
1921 1921 c, g dbSNP:763753426
1942 1942 g, t dbSNP:533413759
1948 1948 a, c dbSNP:144785833
1960 1960 a, t dbSNP:373523144
1973 1973 c, g dbSNP:745311590
1979 1979 -, t dbSNP:757291509
1979 1979 c, t dbSNP:772120884
1983 1983 c, t dbSNP:759613624
1995 1995 c, t dbSNP:773134309
1996 1996 -, c dbSNP:778548782
1997 1997 a, c dbSNP:769966077
1998 1998 -, cacatttaa dbSNP:753753364
2001 2001 a, g dbSNP:370664775
2004 2004 g, t dbSNP:781510219
2008 2008 -, a dbSNP:764153995
2010 2010 a, g dbSNP:768944810
2029 2029 a, g dbSNP:560017021
2036 2036 -, a dbSNP:372684210
2037 2037 c, t dbSNP:547441737
2051 2051 c, t dbSNP:529283663
2063 2063 c, t dbSNP:561827482
2069 2069 c, t dbSNP:543558826
2074 2074 a, c dbSNP:182718582
2140 2140 g, t dbSNP:191052844
2147 2147 a, g dbSNP:545188958
2177 2177 c, g dbSNP:139624482
2188 2188 c, g dbSNP:553245570
2203 2203 a, c dbSNP:3168111

Target ORF information:

RefSeq Version XM_011542798
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens heat shock 70kDa protein 8 (HSPA8), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011542798

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA
CTCACAGGCA TACCTCCTGC ACCCCGAGGT GTTCCTCAGA TTGAAGTCAC TTTTGACATT
GATGCCAATG GTATACTCAA TGTCTCTGCT GTGGACAAGA GTACGGGAAA AGAGAACAAG
ATTACTATCA CTAATGACAA GGGCCGTTTG AGCAAGGAAG ACATTGAACG TATGGTCCAG
GAAGCTGAGA AGTACAAAGC TGAAGATGAG AAGCAGAGGG ACAAGGTGTC ATCCAAGAAT
TCACTTGAGT CCTATGCCTT CAACATGAAA GCAACTGTTG AAGATGAGAA ACTTCAAGGC
AAGATTAACG ATGAGGACAA ACAGAAGATT CTGGACAAGT GTAATGAAAT TATCAACTGG
CTTGATAAGA ATCAGACTGC TGAGAAGGAA GAATTTGAAC ATCAACAGAA AGAGCTGGAG
AAAGTTTGCA ACCCCATCAT CACCAAGCTG TACCAGAGTG CAGGAGGCAT GCCAGGAGGA
ATGCCTGGGG GATTTCCTGG TGGTGGAGCT CCTCCCTCTG GTGGTGCTTC CTCAGGGCCC
ACCATTGAAG AGGTTGATTTA A

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu27538D
Sequence Information ORF Nucleotide Sequence (Length: 1941bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product heat shock cognate 71 kDa protein isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC312904.1, DC395565.1, BC019816.2 and AK222628.1. This sequence is a reference standard in the RefSeqGene project. On Jul 30, 2013 this sequence version replaced gi:342349303. Summary: This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]. Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC016660.1, AK129885.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437, SAMEA962337 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA
CTCACAGGCA TACCTCCTGC ACCCCGAGGT GTTCCTCAGA TTGAAGTCAC TTTTGACATT
GATGCCAATG GTATACTCAA TGTCTCTGCT GTGGACAAGA GTACGGGAAA AGAGAACAAG
ATTACTATCA CTAATGACAA GGGCCGTTTG AGCAAGGAAG ACATTGAACG TATGGTCCAG
GAAGCTGAGA AGTACAAAGC TGAAGATGAG AAGCAGAGGG ACAAGGTGTC ATCCAAGAAT
TCACTTGAGT CCTATGCCTT CAACATGAAA GCAACTGTTG AAGATGAGAA ACTTCAAGGC
AAGATTAACG ATGAGGACAA ACAGAAGATT CTGGACAAGT GTAATGAAAT TATCAACTGG
CTTGATAAGA ATCAGACTGC TGAGAAGGAA GAATTTGAAC ATCAACAGAA AGAGCTGGAG
AAAGTTTGCA ACCCCATCAT CACCAAGCTG TACCAGAGTG CAGGAGGCAT GCCAGGAGGA
ATGCCTGGGG GATTTCCTGG TGGTGGAGCT CCTCCCTCTG GTGGTGCTTC CTCAGGGCCC
ACCATTGAAG AGGTTGATTTA A
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_006588.1
CDS278..2218
Misc Feature(1)170..172(+)
Misc Feature(2)278..2116(+)
Misc Feature(3)281..283(+)
Misc Feature(4)293..1420(+)
Misc Feature(5)305..1375(+)
Misc Feature(6)344..1303(+)
Misc Feature(7)374..1159(+)
Misc Feature(8)731..1252(+)
Misc Feature(9)734..736(+)
Misc Feature(10)734..736(+)
Misc Feature(11)833..1408(+)
Misc Feature(12)1013..1015(+)
Misc Feature(13)1232..1234(+)
Misc Feature(14)1361..1363(+)
Misc Feature(15)1706..1708(+)
Misc Feature(16)1958..1960(+)
Misc Feature(17)2042..2044(+)
Misc Feature(18)2066..2068(+)
Misc Feature(19)2078..2080(+)
Exon (1)1..272
Gene:HSPA8
Gene Synonym:
Exon (2)273..482
Gene:HSPA8
Gene Synonym:
Exon (3)483..688
Gene:HSPA8
Gene Synonym:
Exon (4)689..841
Gene:HSPA8
Gene Synonym:
Exon (5)842..1397
Gene:HSPA8
Gene Synonym:
Exon (6)1398..1600
Gene:HSPA8
Gene Synonym:
Exon (7)1601..1799
Gene:HSPA8
Gene Synonym:
Exon (8)1800..2032
Gene:HSPA8
Gene Synonym:
Exon (9)2033..2460
Gene:HSPA8
Gene Synonym:
Translation
Position Chain Variation Link
4 4 a, g dbSNP:768427133
6 6 g, t dbSNP:185649678
127 127 -, g dbSNP:377729208
128 128 -, cagca dbSNP:377098901
129 129 a, c dbSNP:574052381
164 164 a, g dbSNP:555596864
175 175 a, g dbSNP:536022837
189 189 c, t dbSNP:568739504
191 191 c, g dbSNP:192415561
202 202 c, t dbSNP:556837570
209 209 c, g dbSNP:752747672
214 214 c, g, t dbSNP:186830244
221 221 c, t dbSNP:780539154
222 222 c, g dbSNP:182109261
225 225 a, g dbSNP:190423677
226 226 c, g dbSNP:546530753
227 227 a, g dbSNP:2276077
229 229 g, t dbSNP:567317909
231 231 a, t dbSNP:2276076
233 233 -, t dbSNP:565365955
238 238 c, g, t dbSNP:531101772
240 240 a, t dbSNP:563919080
242 242 a, c, t dbSNP:2276075
243 243 c, t dbSNP:11551600
251 251 a, t dbSNP:187119724
267 267 c, t dbSNP:1136141
268 268 c, g dbSNP:2276074
269 269 c, g dbSNP:573986079
274 274 a, t dbSNP:745900938
277 277 c, t dbSNP:774277451
286 286 a, g dbSNP:771060966
291 291 c, t dbSNP:749485084
293 293 g, t dbSNP:778022948
301 301 c, t dbSNP:770097995
313 313 c, t dbSNP:536070549
319 319 c, t dbSNP:144123192
346 346 c, t dbSNP:755455419
349 349 a, g dbSNP:752166960
355 355 c, g dbSNP:779708153
371 371 g, t dbSNP:11551602
388 388 c, t dbSNP:758030256
397 397 c, t dbSNP:750047710
398 398 c, t dbSNP:75739900
399 399 a, g dbSNP:765012279
403 403 c, g, t dbSNP:369851462
406 406 c, g, t dbSNP:760522151
411 411 c, g, t dbSNP:201477863
412 412 a, g dbSNP:775162274
416 416 a, g dbSNP:770980780
422 422 a, c dbSNP:763047269
430 430 a, c, t dbSNP:376156706
439 439 c, g, t dbSNP:767193605
442 442 a, c dbSNP:781396927
472 472 c, t dbSNP:769131425
490 490 a, g dbSNP:776675548
494 494 c, g dbSNP:764381465
496 496 g, t dbSNP:761062129
505 505 a, c dbSNP:775980264
529 529 a, g dbSNP:570819499
550 550 c, g dbSNP:746344123
553 553 c, t dbSNP:775043697
559 559 a, g dbSNP:770300995
562 562 g, t dbSNP:748864676
564 564 a, g dbSNP:777228127
565 565 c, t dbSNP:11551601
571 571 c, t dbSNP:755808417
574 574 c, t dbSNP:201223120
583 583 a, g dbSNP:780961303
586 586 c, g, t dbSNP:751477695
588 588 a, g dbSNP:766369333
593 593 c, g dbSNP:200601870
597 597 a, g dbSNP:748008431
598 598 c, t dbSNP:371835507
603 603 a, g dbSNP:781044959
604 604 a, c dbSNP:764136878
607 607 a, g dbSNP:760971468
610 610 c, g, t dbSNP:140536048
623 623 a, c dbSNP:11551608
625 625 a, g dbSNP:759998595
647 647 c, t dbSNP:774683371
652 652 a, g dbSNP:147610984
655 655 a, g dbSNP:374961418
661 661 a, g dbSNP:772769841
670 670 a, g dbSNP:769361497
671 671 c, g dbSNP:747769427
672 672 a, g dbSNP:780873363
679 679 c, t dbSNP:768398499
690 690 c, g dbSNP:774366889
694 694 c, t dbSNP:771009079
695 695 -, a dbSNP:777175548
696 696 a, c, g dbSNP:141156763
699 699 a, g dbSNP:569651128
702 702 c, t dbSNP:755052827
730 730 c, t dbSNP:747234098
750 750 c, t dbSNP:11551606
763 763 a, g dbSNP:145779755
772 772 a, c, t dbSNP:141917932
781 781 c, t dbSNP:765647043
799 799 c, t dbSNP:757755949
826 826 c, t dbSNP:148609753
843 843 g, t dbSNP:199619043
844 844 g, t dbSNP:200486073
847 847 a, g dbSNP:773246178
848 848 a, g dbSNP:769624839
849 849 c, g, t dbSNP:775427523
850 850 a, g dbSNP:772233016
859 859 a, c, t dbSNP:779126635
865 865 a, c, t dbSNP:749654902
871 871 c, t dbSNP:573467766
874 874 c, t dbSNP:376403473
902 902 a, g dbSNP:778283472
910 910 c, t dbSNP:756568266
934 934 a, c dbSNP:753317621
941 941 a, g dbSNP:11551599
982 982 c, t dbSNP:201712472
985 985 a, g dbSNP:373526651
993 993 a, g dbSNP:536669943
994 994 c, t dbSNP:762772078
1000 1000 c, t dbSNP:750169512
1004 1004 g, t dbSNP:765204439
1006 1006 a, c, t dbSNP:761855165
1012 1012 c, t dbSNP:775590979
1018 1018 c, g dbSNP:761623666
1027 1027 a, g dbSNP:776678845
1033 1033 c, t dbSNP:369763812
1034 1034 a, g dbSNP:759639896
1036 1036 a, c, t dbSNP:150964023
1039 1039 c, t dbSNP:772077059
1051 1051 a, g dbSNP:749572958
1060 1060 a, g dbSNP:777998627
1064 1064 c, t dbSNP:770238029
1066 1066 c, t dbSNP:759775156
1067 1067 c, t dbSNP:748585744
1069 1069 c, t dbSNP:143480238
1082 1082 c, t dbSNP:11551604
1091 1091 c, t dbSNP:11551605
1096 1096 c, g, t dbSNP:774484203
1097 1097 c, g dbSNP:751121248
1099 1099 c, t dbSNP:779796438
1105 1105 c, t dbSNP:758117987
1122 1122 c, t dbSNP:750129947
1129 1129 c, t dbSNP:148846437
1138 1138 c, g, t dbSNP:551131709
1141 1141 c, t dbSNP:764020103
1148 1148 a, g dbSNP:532778326
1150 1150 c, t dbSNP:538018718
1153 1153 c, t dbSNP:766515357
1161 1161 c, t dbSNP:763133730
1164 1164 c, t dbSNP:773536032
1171 1171 c, t dbSNP:200035932
1177 1177 c, t dbSNP:749718428
1183 1183 c, t dbSNP:748576941
1190 1190 c, g, t dbSNP:778115074
1192 1192 c, g dbSNP:145580523
1197 1197 c, t dbSNP:747578888
1198 1198 c, t dbSNP:143373443
1217 1217 c, t dbSNP:758028103
1225 1225 a, g dbSNP:745458075
1255 1255 a, g dbSNP:770224594
1258 1258 c, t dbSNP:376828215
1261 1261 a, g dbSNP:753679532
1264 1264 a, g dbSNP:139992369
1266 1266 a, t dbSNP:373235233
1268 1268 a, g dbSNP:752672546
1270 1270 g, t dbSNP:766425028
1278 1278 c, t dbSNP:763116637
1279 1279 c, t dbSNP:146503730
1282 1282 c, t dbSNP:115882568
1322 1322 c, t dbSNP:762028517
1327 1327 c, g dbSNP:777124482
1333 1333 c, t dbSNP:139484659
1335 1335 a, t dbSNP:747492962
1336 1336 c, t dbSNP:150854686
1339 1339 c, t dbSNP:771663386
1342 1342 c, t dbSNP:529656968
1351 1351 a, g dbSNP:142431740
1356 1356 a, g dbSNP:756920138
1363 1363 c, t dbSNP:748958068
1369 1369 c, t dbSNP:148737498
1381 1381 g, t dbSNP:577357118
1388 1388 -, tt dbSNP:771981132
1398 1398 c, t dbSNP:746781077
1402 1402 a, c dbSNP:775435579
1417 1417 a, g dbSNP:772121271
1426 1426 c, t dbSNP:531182299
1435 1435 a, g dbSNP:745866854
1451 1451 c, t dbSNP:142113865
1453 1453 a, g dbSNP:756309650
1456 1456 c, t dbSNP:748355748
1468 1468 c, t dbSNP:781302641
1472 1472 c, t dbSNP:755304850
1477 1477 c, t dbSNP:144361240
1502 1502 a, g dbSNP:372928718
1513 1513 a, c, t dbSNP:371408292
1531 1531 c, t dbSNP:550170308
1534 1534 c, t dbSNP:368207632
1537 1537 c, t dbSNP:761340541
1546 1546 a, g dbSNP:139016926
1557 1557 c, t dbSNP:763663403
1567 1567 c, g dbSNP:760433396
1576 1576 c, t dbSNP:775143184
1578 1578 a, g dbSNP:772031159
1582 1582 a, g dbSNP:759523701
1585 1585 c, t dbSNP:757222670
1588 1588 g, t dbSNP:775451393
1591 1591 a, g dbSNP:774454573
1612 1612 c, t dbSNP:767284605
1627 1627 a, g dbSNP:553450668
1639 1639 c, t dbSNP:759347844
1642 1642 c, g dbSNP:751387334
1645 1645 a, t dbSNP:377257990
1652 1652 a, g dbSNP:11551598
1661 1661 a, g dbSNP:746441724
1674 1674 c, t dbSNP:773253340
1675 1675 g, t dbSNP:764034057
1702 1702 a, g dbSNP:760810506
1711 1711 c, t dbSNP:775629110
1717 1717 c, t dbSNP:772264576
1726 1726 c, t dbSNP:199504970
1732 1732 a, c dbSNP:1064585
1735 1735 c, t dbSNP:771376397
1737 1737 a, g dbSNP:749707173
1741 1741 c, g dbSNP:571307052
1744 1744 a, t dbSNP:778373831
1756 1756 a, g dbSNP:138544510
1762 1762 a, g dbSNP:147529697
1776 1776 a, g dbSNP:780908558
1783 1783 c, g, t dbSNP:111774985
1788 1788 c, g dbSNP:766069802
1798 1798 a, g dbSNP:758237764
1827 1827 a, g, t dbSNP:771746646
1828 1828 g, t dbSNP:536065122
1832 1832 a, g dbSNP:778743630
1840 1840 a, g dbSNP:143434494
1847 1847 a, g dbSNP:753733140
1849 1849 a, g dbSNP:371945853
1867 1867 a, g dbSNP:137943836
1874 1874 a, c dbSNP:751646265
1875 1875 a, g dbSNP:766437819
1878 1878 a, c dbSNP:763204793
1885 1885 a, g dbSNP:773460738
1891 1891 a, c, g, t dbSNP:777216036
1905 1905 -, agtc dbSNP:751625114
1910 1910 -, ttc dbSNP:766511510
1911 1911 a, g dbSNP:769269163
1913 1913 g, t dbSNP:761149194
1914 1914 -, cc dbSNP:763022802
1918 1918 c, g dbSNP:775032110
1920 1920 -, gg dbSNP:773074126
1922 1922 a, g dbSNP:568778183
1924 1924 -, gaa dbSNP:769802670
1926 1926 a, g dbSNP:745475251
1928 1928 -, gact dbSNP:761484393
1931 1931 a, g dbSNP:778459169
1934 1934 a, g dbSNP:770645829
1939 1939 -, agatga dbSNP:776539014
1952 1952 c, g dbSNP:749093478
1961 1961 a, g dbSNP:777603150
1965 1965 a, g dbSNP:756041393
1966 1966 a, c, t dbSNP:199882006
1967 1967 a, g dbSNP:765561454
1969 1969 c, t dbSNP:113013616
1984 1984 a, g dbSNP:750621530
1990 1990 a, g dbSNP:765564967
1996 1996 a, g dbSNP:757641811
1998 1998 c, g dbSNP:754246861
2009 2009 a, g dbSNP:764589322
2014 2014 c, t dbSNP:761220214
2026 2026 a, g dbSNP:775881301
2038 2038 c, g, t dbSNP:4802
2050 2050 a, g dbSNP:761588256
2056 2056 a, g dbSNP:112636139
2059 2059 c, t dbSNP:41487746
2060 2060 a, c dbSNP:776255814
2062 2062 a, g dbSNP:768540563
2063 2063 a, c dbSNP:775363477
2069 2069 a, g dbSNP:760638348
2072 2072 c, t dbSNP:145240324
2089 2089 c, t dbSNP:370796977
2091 2091 c, t dbSNP:377681653
2092 2092 a, c, t dbSNP:118171965
2101 2101 c, t dbSNP:188408273
2106 2106 g, t dbSNP:781388678
2110 2110 c, t dbSNP:755360656
2118 2118 c, t dbSNP:752022565
2123 2123 a, g dbSNP:780546314
2136 2136 a, g dbSNP:757849635
2152 2152 c, t dbSNP:780073872
2158 2158 c, t dbSNP:201479266
2159 2159 a, g dbSNP:764872570
2164 2164 a, g dbSNP:758520548
2166 2166 c, t dbSNP:753546825
2173 2173 c, g dbSNP:763753426
2194 2194 g, t dbSNP:533413759
2200 2200 a, c dbSNP:144785833
2212 2212 a, t dbSNP:373523144
2225 2225 c, g dbSNP:745311590
2231 2231 -, t dbSNP:757291509
2231 2231 c, t dbSNP:772120884
2235 2235 c, t dbSNP:759613624
2247 2247 c, t dbSNP:773134309
2248 2248 -, c dbSNP:778548782
2249 2249 a, c dbSNP:769966077
2250 2250 -, cacatttaa dbSNP:753753364
2253 2253 a, g dbSNP:370664775
2256 2256 g, t dbSNP:781510219
2260 2260 -, a dbSNP:764153995
2262 2262 a, g dbSNP:768944810
2281 2281 a, g dbSNP:560017021
2288 2288 -, a dbSNP:372684210
2289 2289 c, t dbSNP:547441737
2303 2303 c, t dbSNP:529283663
2315 2315 c, t dbSNP:561827482
2321 2321 c, t dbSNP:543558826
2326 2326 a, c dbSNP:182718582
2392 2392 g, t dbSNP:191052844
2399 2399 a, g dbSNP:545188958
2429 2429 c, g dbSNP:139624482
2440 2440 c, g dbSNP:553245570
2455 2455 a, c dbSNP:3168111

Target ORF information:

RefSeq Version NM_006597
Organism Homo sapiens (human)
Definition Homo sapiens heat shock 70kDa protein 8 (HSPA8), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_006597

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA
CTCACAGGCA TACCTCCTGC ACCCCGAGGT GTTCCTCAGA TTGAAGTCAC TTTTGACATT
GATGCCAATG GTATACTCAA TGTCTCTGCT GTGGACAAGA GTACGGGAAA AGAGAACAAG
ATTACTATCA CTAATGACAA GGGCCGTTTG AGCAAGGAAG ACATTGAACG TATGGTCCAG
GAAGCTGAGA AGTACAAAGC TGAAGATGAG AAGCAGAGGG ACAAGGTGTC ATCCAAGAAT
TCACTTGAGT CCTATGCCTT CAACATGAAA GCAACTGTTG AAGATGAGAA ACTTCAAGGC
AAGATTAACG ATGAGGACAA ACAGAAGATT CTGGACAAGT GTAATGAAAT TATCAACTGG
CTTGATAAGA ATCAGACTGC TGAGAAGGAA GAATTTGAAC ATCAACAGAA AGAGCTGGAG
AAAGTTTGCA ACCCCATCAT CACCAAGCTG TACCAGAGTG CAGGAGGCAT GCCAGGAGGA
ATGCCTGGGG GATTTCCTGG TGGTGGAGCT CCTCCCTCTG GTGGTGCTTC CTCAGGGCCC
ACCATTGAAG AGGTTGATTTA A

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu27531D
Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product heat shock cognate 71 kDa protein isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC312904.1, DC395565.1, AB034951.1 and AK222628.1. On or before Aug 31, 2013 this sequence version replaced gi:530398064, gi:342349304. Summary: This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]. Transcript Variant: This variant (2) uses alternate splice sites, and is missing an exon in the 3' coding region compared to variant 1. However, it maintains the reading frame, and encodes a shorter isoform (2) missing an internal protein segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222628.1, AB034951.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA
CTCACAGGCA TGCCAGGAGG AATGCCTGGG GGATTTCCTG GTGGTGGAGC TCCTCCCTCT
GGTGGTGCTT CCTCAGGGCC CACCATTGAA GAGGTTGATTT AA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_694881.1
CDS278..1759
Misc Feature(1)170..172(+)
Misc Feature(2)281..283(+)
Misc Feature(3)293..1420(+)
Misc Feature(4)296..1396(+)
Misc Feature(5)305..1375(+)
Misc Feature(6)344..1303(+)
Misc Feature(7)374..1159(+)
Misc Feature(8)731..1252(+)
Misc Feature(9)734..736(+)
Misc Feature(10)734..736(+)
Misc Feature(11)833..1408(+)
Misc Feature(12)1013..1015(+)
Misc Feature(13)1232..1234(+)
Misc Feature(14)1361..1363(+)
Exon (1)1..272
Gene:HSPA8
Gene Synonym:
Exon (2)273..482
Gene:HSPA8
Gene Synonym:
Exon (3)483..688
Gene:HSPA8
Gene Synonym:
Exon (4)689..841
Gene:HSPA8
Gene Synonym:
Exon (5)842..1397
Gene:HSPA8
Gene Synonym:
Exon (6)1398..1600
Gene:HSPA8
Gene Synonym:
Exon (7)1601..1664
Gene:HSPA8
Gene Synonym:
Exon (8)1665..2001
Gene:HSPA8
Gene Synonym:
Translation
Position Chain Variation Link
4 4 a, g dbSNP:768427133
6 6 g, t dbSNP:185649678
127 127 -, g dbSNP:377729208
128 128 -, cagca dbSNP:377098901
129 129 a, c dbSNP:574052381
164 164 a, g dbSNP:555596864
175 175 a, g dbSNP:536022837
189 189 c, t dbSNP:568739504
191 191 c, g dbSNP:192415561
202 202 c, t dbSNP:556837570
209 209 c, g dbSNP:752747672
214 214 c, g, t dbSNP:186830244
221 221 c, t dbSNP:780539154
222 222 c, g dbSNP:182109261
225 225 a, g dbSNP:190423677
226 226 c, g dbSNP:546530753
227 227 a, g dbSNP:2276077
229 229 g, t dbSNP:567317909
231 231 a, t dbSNP:2276076
233 233 -, t dbSNP:565365955
238 238 c, g, t dbSNP:531101772
240 240 a, t dbSNP:563919080
242 242 a, c, t dbSNP:2276075
243 243 c, t dbSNP:11551600
251 251 a, t dbSNP:187119724
267 267 c, t dbSNP:1136141
268 268 c, g dbSNP:2276074
269 269 c, g dbSNP:573986079
274 274 a, t dbSNP:745900938
277 277 c, t dbSNP:774277451
286 286 a, g dbSNP:771060966
291 291 c, t dbSNP:749485084
293 293 g, t dbSNP:778022948
301 301 c, t dbSNP:770097995
313 313 c, t dbSNP:536070549
319 319 c, t dbSNP:144123192
346 346 c, t dbSNP:755455419
349 349 a, g dbSNP:752166960
355 355 c, g dbSNP:779708153
371 371 g, t dbSNP:11551602
388 388 c, t dbSNP:758030256
397 397 c, t dbSNP:750047710
398 398 c, t dbSNP:75739900
399 399 a, g dbSNP:765012279
403 403 c, g, t dbSNP:369851462
406 406 c, g, t dbSNP:760522151
411 411 c, g, t dbSNP:201477863
412 412 a, g dbSNP:775162274
416 416 a, g dbSNP:770980780
422 422 a, c dbSNP:763047269
430 430 a, c, t dbSNP:376156706
439 439 c, g, t dbSNP:767193605
442 442 a, c dbSNP:781396927
472 472 c, t dbSNP:769131425
490 490 a, g dbSNP:776675548
494 494 c, g dbSNP:764381465
496 496 g, t dbSNP:761062129
505 505 a, c dbSNP:775980264
529 529 a, g dbSNP:570819499
550 550 c, g dbSNP:746344123
553 553 c, t dbSNP:775043697
559 559 a, g dbSNP:770300995
562 562 g, t dbSNP:748864676
564 564 a, g dbSNP:777228127
565 565 c, t dbSNP:11551601
571 571 c, t dbSNP:755808417
574 574 c, t dbSNP:201223120
583 583 a, g dbSNP:780961303
586 586 c, g, t dbSNP:751477695
588 588 a, g dbSNP:766369333
593 593 c, g dbSNP:200601870
597 597 a, g dbSNP:748008431
598 598 c, t dbSNP:371835507
603 603 a, g dbSNP:781044959
604 604 a, c dbSNP:764136878
607 607 a, g dbSNP:760971468
610 610 c, g, t dbSNP:140536048
623 623 a, c dbSNP:11551608
625 625 a, g dbSNP:759998595
647 647 c, t dbSNP:774683371
652 652 a, g dbSNP:147610984
655 655 a, g dbSNP:374961418
661 661 a, g dbSNP:772769841
670 670 a, g dbSNP:769361497
671 671 c, g dbSNP:747769427
672 672 a, g dbSNP:780873363
679 679 c, t dbSNP:768398499
690 690 c, g dbSNP:774366889
694 694 c, t dbSNP:771009079
695 695 -, a dbSNP:777175548
696 696 a, c, g dbSNP:141156763
699 699 a, g dbSNP:569651128
702 702 c, t dbSNP:755052827
730 730 c, t dbSNP:747234098
750 750 c, t dbSNP:11551606
763 763 a, g dbSNP:145779755
772 772 a, c, t dbSNP:141917932
781 781 c, t dbSNP:765647043
799 799 c, t dbSNP:757755949
826 826 c, t dbSNP:148609753
843 843 g, t dbSNP:199619043
844 844 g, t dbSNP:200486073
847 847 a, g dbSNP:773246178
848 848 a, g dbSNP:769624839
849 849 c, g, t dbSNP:775427523
850 850 a, g dbSNP:772233016
859 859 a, c, t dbSNP:779126635
865 865 a, c, t dbSNP:749654902
871 871 c, t dbSNP:573467766
874 874 c, t dbSNP:376403473
902 902 a, g dbSNP:778283472
910 910 c, t dbSNP:756568266
934 934 a, c dbSNP:753317621
941 941 a, g dbSNP:11551599
982 982 c, t dbSNP:201712472
985 985 a, g dbSNP:373526651
993 993 a, g dbSNP:536669943
994 994 c, t dbSNP:762772078
1000 1000 c, t dbSNP:750169512
1004 1004 g, t dbSNP:765204439
1006 1006 a, c, t dbSNP:761855165
1012 1012 c, t dbSNP:775590979
1018 1018 c, g dbSNP:761623666
1027 1027 a, g dbSNP:776678845
1033 1033 c, t dbSNP:369763812
1034 1034 a, g dbSNP:759639896
1036 1036 a, c, t dbSNP:150964023
1039 1039 c, t dbSNP:772077059
1051 1051 a, g dbSNP:749572958
1060 1060 a, g dbSNP:777998627
1064 1064 c, t dbSNP:770238029
1066 1066 c, t dbSNP:759775156
1067 1067 c, t dbSNP:748585744
1069 1069 c, t dbSNP:143480238
1082 1082 c, t dbSNP:11551604
1091 1091 c, t dbSNP:11551605
1096 1096 c, g, t dbSNP:774484203
1097 1097 c, g dbSNP:751121248
1099 1099 c, t dbSNP:779796438
1105 1105 c, t dbSNP:758117987
1122 1122 c, t dbSNP:750129947
1129 1129 c, t dbSNP:148846437
1138 1138 c, g, t dbSNP:551131709
1141 1141 c, t dbSNP:764020103
1148 1148 a, g dbSNP:532778326
1150 1150 c, t dbSNP:538018718
1153 1153 c, t dbSNP:766515357
1161 1161 c, t dbSNP:763133730
1164 1164 c, t dbSNP:773536032
1171 1171 c, t dbSNP:200035932
1177 1177 c, t dbSNP:749718428
1183 1183 c, t dbSNP:748576941
1190 1190 c, g, t dbSNP:778115074
1192 1192 c, g dbSNP:145580523
1197 1197 c, t dbSNP:747578888
1198 1198 c, t dbSNP:143373443
1217 1217 c, t dbSNP:758028103
1225 1225 a, g dbSNP:745458075
1255 1255 a, g dbSNP:770224594
1258 1258 c, t dbSNP:376828215
1261 1261 a, g dbSNP:753679532
1264 1264 a, g dbSNP:139992369
1266 1266 a, t dbSNP:373235233
1268 1268 a, g dbSNP:752672546
1270 1270 g, t dbSNP:766425028
1278 1278 c, t dbSNP:763116637
1279 1279 c, t dbSNP:146503730
1282 1282 c, t dbSNP:115882568
1322 1322 c, t dbSNP:762028517
1327 1327 c, g dbSNP:777124482
1333 1333 c, t dbSNP:139484659
1335 1335 a, t dbSNP:747492962
1336 1336 c, t dbSNP:150854686
1339 1339 c, t dbSNP:771663386
1342 1342 c, t dbSNP:529656968
1351 1351 a, g dbSNP:142431740
1356 1356 a, g dbSNP:756920138
1363 1363 c, t dbSNP:748958068
1369 1369 c, t dbSNP:148737498
1381 1381 g, t dbSNP:577357118
1388 1388 -, tt dbSNP:771981132
1398 1398 c, t dbSNP:746781077
1402 1402 a, c dbSNP:775435579
1417 1417 a, g dbSNP:772121271
1426 1426 c, t dbSNP:531182299
1435 1435 a, g dbSNP:745866854
1451 1451 c, t dbSNP:142113865
1453 1453 a, g dbSNP:756309650
1456 1456 c, t dbSNP:748355748
1468 1468 c, t dbSNP:781302641
1472 1472 c, t dbSNP:755304850
1477 1477 c, t dbSNP:144361240
1502 1502 a, g dbSNP:372928718
1513 1513 a, c, t dbSNP:371408292
1531 1531 c, t dbSNP:550170308
1534 1534 c, t dbSNP:368207632
1537 1537 c, t dbSNP:761340541
1546 1546 a, g dbSNP:139016926
1557 1557 c, t dbSNP:763663403
1567 1567 c, g dbSNP:760433396
1576 1576 c, t dbSNP:775143184
1578 1578 a, g dbSNP:772031159
1582 1582 a, g dbSNP:759523701
1585 1585 c, t dbSNP:757222670
1588 1588 g, t dbSNP:775451393
1591 1591 a, g dbSNP:774454573
1612 1612 c, t dbSNP:767284605
1627 1627 a, g dbSNP:553450668
1639 1639 c, t dbSNP:759347844
1642 1642 c, g dbSNP:751387334
1645 1645 a, t dbSNP:377257990
1652 1652 a, g dbSNP:11551598
1661 1661 a, g dbSNP:746441724
1677 1677 a, g dbSNP:757849635
1693 1693 c, t dbSNP:780073872
1699 1699 c, t dbSNP:201479266
1700 1700 a, g dbSNP:764872570
1705 1705 a, g dbSNP:758520548
1707 1707 c, t dbSNP:753546825
1714 1714 c, g dbSNP:763753426
1735 1735 g, t dbSNP:533413759
1741 1741 a, c dbSNP:144785833
1753 1753 a, t dbSNP:373523144
1766 1766 c, g dbSNP:745311590
1772 1772 -, t dbSNP:757291509
1772 1772 c, t dbSNP:772120884
1776 1776 c, t dbSNP:759613624
1788 1788 c, t dbSNP:773134309
1789 1789 -, c dbSNP:778548782
1790 1790 a, c dbSNP:769966077
1791 1791 -, cacatttaa dbSNP:753753364
1794 1794 a, g dbSNP:370664775
1797 1797 g, t dbSNP:781510219
1801 1801 -, a dbSNP:764153995
1803 1803 a, g dbSNP:768944810
1822 1822 a, g dbSNP:560017021
1829 1829 -, a dbSNP:372684210
1830 1830 c, t dbSNP:547441737
1844 1844 c, t dbSNP:529283663
1856 1856 c, t dbSNP:561827482
1862 1862 c, t dbSNP:543558826
1867 1867 a, c dbSNP:182718582
1933 1933 g, t dbSNP:191052844
1940 1940 a, g dbSNP:545188958
1970 1970 c, g dbSNP:139624482
1981 1981 c, g dbSNP:553245570
1996 1996 a, c dbSNP:3168111

Target ORF information:

RefSeq Version NM_153201
Organism Homo sapiens (human)
Definition Homo sapiens heat shock 70kDa protein 8 (HSPA8), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_153201

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA
CTCACAGGCA TGCCAGGAGG AATGCCTGGG GGATTTCCTG GTGGTGGAGC TCCTCCCTCT
GGTGGTGCTT CCTCAGGGCC CACCATTGAA GAGGTTGATTT AA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.