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HSPA8 cDNA ORF clone, Homo sapiens (human)

Gene Symbol HSPA8
Entrez Gene ID 3312
Full Name heat shock 70kDa protein 8
Synonyms HEL-33, HEL-S-72p, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP-1, LAP1, NIP71
General protein information
Preferred Names
heat shock cognate 71 kDa protein
Names
heat shock cognate 71 kDa protein
LPS-associated protein 1
heat shock 70kd protein 10
heat shock cognate protein 54
constitutive heat shock protein 70
lipopolysaccharide-associated protein 1
N-myristoyltransferase inhibitor protein 71
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11q24.1

Summary This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]. lac of sum
Disorder MIM:

600816


mRNA and Protein(s)

mRNA Protein Name
XM_011542798 XP_011541100 heat shock cognate 71 kDa protein isoform X1
NM_006597 NP_006588 heat shock cognate 71 kDa protein isoform 1
NM_153201 NP_694881 heat shock cognate 71 kDa protein isoform 2



Homo sapiens (human) HSPA8 NP_006588.1
Pan troglodytes (chimpanzee) HSPA8 XP_003313441.2
Macaca mulatta (Rhesus monkey) HSPA8 XP_002799868.1
Canis lupus familiaris (dog) HSPA8 XP_005619707.1
Bos taurus (cattle) HSPA8 NP_776770.2
Mus musculus (house mouse) Hspa8 NP_112442.2
Rattus norvegicus (Norway rat) Hspa8 NP_077327.1
Gallus gallus (chicken) HSPA8 NP_990334.1
Danio rerio (zebrafish) hspa8 NP_001103873.1
Drosophila melanogaster (fruit fly) Hsc70-1 NP_524063.1
Caenorhabditis elegans hsp-1 NP_503068.1
Arabidopsis thaliana (thale cress) AT3G09440 NP_001189847.1
Xenopus (Silurana) tropicalis (western clawed frog) hspa8 XP_004916187.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HSPA8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSPA8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu27538
XM_011542798 PREDICTED: Homo sapiens heat shock 70kDa protein 8 (HSPA8), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
OHu27538
NM_006597 Homo sapiens heat shock 70kDa protein 8 (HSPA8), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
OHu27531 NM_153201 Homo sapiens heat shock 70kDa protein 8 (HSPA8), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu27538
Clone ID Related Accession (Same CDS sequence) NM_006597 , XM_011542798
Accession Version XM_011542798.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1941bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product heat shock cognate 71 kDa protein isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_033899.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA
CTCACAGGCA TACCTCCTGC ACCCCGAGGT GTTCCTCAGA TTGAAGTCAC TTTTGACATT
GATGCCAATG GTATACTCAA TGTCTCTGCT GTGGACAAGA GTACGGGAAA AGAGAACAAG
ATTACTATCA CTAATGACAA GGGCCGTTTG AGCAAGGAAG ACATTGAACG TATGGTCCAG
GAAGCTGAGA AGTACAAAGC TGAAGATGAG AAGCAGAGGG ACAAGGTGTC ATCCAAGAAT
TCACTTGAGT CCTATGCCTT CAACATGAAA GCAACTGTTG AAGATGAGAA ACTTCAAGGC
AAGATTAACG ATGAGGACAA ACAGAAGATT CTGGACAAGT GTAATGAAAT TATCAACTGG
CTTGATAAGA ATCAGACTGC TGAGAAGGAA GAATTTGAAC ATCAACAGAA AGAGCTGGAG
AAAGTTTGCA ACCCCATCAT CACCAAGCTG TACCAGAGTG CAGGAGGCAT GCCAGGAGGA
ATGCCTGGGG GATTTCCTGG TGGTGGAGCT CCTCCCTCTG GTGGTGCTTC CTCAGGGCCC
ACCATTGAAG AGGTTGATTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011541100.1
CDS26..1966
Misc Feature(1)26..1864(+)
Misc Feature(2)41..1168(+)
Misc Feature(3)53..1123(+)
Misc Feature(4)92..1051(+)
Misc Feature(5)122..907(+)
Misc Feature(6)479..1000(+)
Translation

Target ORF information:

RefSeq Version XM_011542798
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens heat shock 70kDa protein 8 (HSPA8), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011542798

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA
CTCACAGGCA TACCTCCTGC ACCCCGAGGT GTTCCTCAGA TTGAAGTCAC TTTTGACATT
GATGCCAATG GTATACTCAA TGTCTCTGCT GTGGACAAGA GTACGGGAAA AGAGAACAAG
ATTACTATCA CTAATGACAA GGGCCGTTTG AGCAAGGAAG ACATTGAACG TATGGTCCAG
GAAGCTGAGA AGTACAAAGC TGAAGATGAG AAGCAGAGGG ACAAGGTGTC ATCCAAGAAT
TCACTTGAGT CCTATGCCTT CAACATGAAA GCAACTGTTG AAGATGAGAA ACTTCAAGGC
AAGATTAACG ATGAGGACAA ACAGAAGATT CTGGACAAGT GTAATGAAAT TATCAACTGG
CTTGATAAGA ATCAGACTGC TGAGAAGGAA GAATTTGAAC ATCAACAGAA AGAGCTGGAG
AAAGTTTGCA ACCCCATCAT CACCAAGCTG TACCAGAGTG CAGGAGGCAT GCCAGGAGGA
ATGCCTGGGG GATTTCCTGG TGGTGGAGCT CCTCCCTCTG GTGGTGCTTC CTCAGGGCCC
ACCATTGAAG AGGTTGATTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27538
Clone ID Related Accession (Same CDS sequence) NM_006597 , XM_011542798
Accession Version NM_006597.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1941bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product heat shock cognate 71 kDa protein isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC312904.1, DC395565.1, BC019816.2 and AK222628.1. This sequence is a reference standard in the RefSeqGene project. On Jul 30, 2013 this sequence version replaced gi:342349303. Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC016660.1, AK129885.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437, SAMEA962337 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA
CTCACAGGCA TACCTCCTGC ACCCCGAGGT GTTCCTCAGA TTGAAGTCAC TTTTGACATT
GATGCCAATG GTATACTCAA TGTCTCTGCT GTGGACAAGA GTACGGGAAA AGAGAACAAG
ATTACTATCA CTAATGACAA GGGCCGTTTG AGCAAGGAAG ACATTGAACG TATGGTCCAG
GAAGCTGAGA AGTACAAAGC TGAAGATGAG AAGCAGAGGG ACAAGGTGTC ATCCAAGAAT
TCACTTGAGT CCTATGCCTT CAACATGAAA GCAACTGTTG AAGATGAGAA ACTTCAAGGC
AAGATTAACG ATGAGGACAA ACAGAAGATT CTGGACAAGT GTAATGAAAT TATCAACTGG
CTTGATAAGA ATCAGACTGC TGAGAAGGAA GAATTTGAAC ATCAACAGAA AGAGCTGGAG
AAAGTTTGCA ACCCCATCAT CACCAAGCTG TACCAGAGTG CAGGAGGCAT GCCAGGAGGA
ATGCCTGGGG GATTTCCTGG TGGTGGAGCT CCTCCCTCTG GTGGTGCTTC CTCAGGGCCC
ACCATTGAAG AGGTTGATTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006588.1
CDS278..2218
Misc Feature(1)170..172(+)
Misc Feature(2)278..2116(+)
Misc Feature(3)281..283(+)
Misc Feature(4)293..1420(+)
Misc Feature(5)305..1375(+)
Misc Feature(6)344..1303(+)
Misc Feature(7)374..1159(+)
Misc Feature(8)731..1252(+)
Misc Feature(9)734..736(+)
Misc Feature(10)734..736(+)
Misc Feature(11)833..1408(+)
Misc Feature(12)1013..1015(+)
Misc Feature(13)1232..1234(+)
Misc Feature(14)1361..1363(+)
Misc Feature(15)1706..1708(+)
Misc Feature(16)1958..1960(+)
Misc Feature(17)2042..2044(+)
Misc Feature(18)2066..2068(+)
Misc Feature(19)2078..2080(+)
Exon (1)1..272
Gene:HSPA8
Gene Synonym:
Exon (2)273..482
Gene:HSPA8
Gene Synonym:
Exon (3)483..688
Gene:HSPA8
Gene Synonym:
Exon (4)689..841
Gene:HSPA8
Gene Synonym:
Exon (5)842..1397
Gene:HSPA8
Gene Synonym:
Exon (6)1398..1600
Gene:HSPA8
Gene Synonym:
Exon (7)1601..1799
Gene:HSPA8
Gene Synonym:
Exon (8)1800..2032
Gene:HSPA8
Gene Synonym:
Exon (9)2033..2460
Gene:HSPA8
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_006597
Organism Homo sapiens (human)
Definition Homo sapiens heat shock 70kDa protein 8 (HSPA8), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006597

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA
CTCACAGGCA TACCTCCTGC ACCCCGAGGT GTTCCTCAGA TTGAAGTCAC TTTTGACATT
GATGCCAATG GTATACTCAA TGTCTCTGCT GTGGACAAGA GTACGGGAAA AGAGAACAAG
ATTACTATCA CTAATGACAA GGGCCGTTTG AGCAAGGAAG ACATTGAACG TATGGTCCAG
GAAGCTGAGA AGTACAAAGC TGAAGATGAG AAGCAGAGGG ACAAGGTGTC ATCCAAGAAT
TCACTTGAGT CCTATGCCTT CAACATGAAA GCAACTGTTG AAGATGAGAA ACTTCAAGGC
AAGATTAACG ATGAGGACAA ACAGAAGATT CTGGACAAGT GTAATGAAAT TATCAACTGG
CTTGATAAGA ATCAGACTGC TGAGAAGGAA GAATTTGAAC ATCAACAGAA AGAGCTGGAG
AAAGTTTGCA ACCCCATCAT CACCAAGCTG TACCAGAGTG CAGGAGGCAT GCCAGGAGGA
ATGCCTGGGG GATTTCCTGG TGGTGGAGCT CCTCCCTCTG GTGGTGCTTC CTCAGGGCCC
ACCATTGAAG AGGTTGATTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27531
Clone ID Related Accession (Same CDS sequence) NM_153201
Accession Version NM_153201.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product heat shock cognate 71 kDa protein isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC312904.1, DC395565.1, AB034951.1 and AK222628.1. On or before Aug 31, 2013 this sequence version replaced gi:530398064, gi:342349304. Transcript Variant: This variant (2) uses alternate splice sites, and is missing an exon in the 3' coding region compared to variant 1. However, it maintains the reading frame, and encodes a shorter isoform (2) missing an internal protein segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222628.1, AB034951.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA
CTCACAGGCA TGCCAGGAGG AATGCCTGGG GGATTTCCTG GTGGTGGAGC TCCTCCCTCT
GGTGGTGCTT CCTCAGGGCC CACCATTGAA GAGGTTGATT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_694881.1
CDS278..1759
Misc Feature(1)170..172(+)
Misc Feature(2)281..283(+)
Misc Feature(3)293..1420(+)
Misc Feature(4)296..1396(+)
Misc Feature(5)305..1375(+)
Misc Feature(6)344..1303(+)
Misc Feature(7)374..1159(+)
Misc Feature(8)731..1252(+)
Misc Feature(9)734..736(+)
Misc Feature(10)734..736(+)
Misc Feature(11)833..1408(+)
Misc Feature(12)1013..1015(+)
Misc Feature(13)1232..1234(+)
Misc Feature(14)1361..1363(+)
Exon (1)1..272
Gene:HSPA8
Gene Synonym:
Exon (2)273..482
Gene:HSPA8
Gene Synonym:
Exon (3)483..688
Gene:HSPA8
Gene Synonym:
Exon (4)689..841
Gene:HSPA8
Gene Synonym:
Exon (5)842..1397
Gene:HSPA8
Gene Synonym:
Exon (6)1398..1600
Gene:HSPA8
Gene Synonym:
Exon (7)1601..1664
Gene:HSPA8
Gene Synonym:
Exon (8)1665..2001
Gene:HSPA8
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_153201
Organism Homo sapiens (human)
Definition Homo sapiens heat shock 70kDa protein 8 (HSPA8), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_153201

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA
CTCACAGGCA TGCCAGGAGG AATGCCTGGG GGATTTCCTG GTGGTGGAGC TCCTCCCTCT
GGTGGTGCTT CCTCAGGGCC CACCATTGAA GAGGTTGATT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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