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IDH1 isocitrate dehydrogenase 1 (NADP+), soluble [Homo sapiens (human)]

Gene Symbol IDH1
Entrez Gene ID 3417
Full Name isocitrate dehydrogenase 1 (NADP+), soluble
Synonyms HEL-216, HEL-S-26, IDCD, IDH, IDP, IDPC, PICD
General protein information
Preferred Names
isocitrate dehydrogenase [NADP] cytoplasmic
Names
isocitrate dehydrogenase [NADP] cytoplasmic
NADP(+)-specific ICDH
oxalosuccinate decarboxylase
NADP-dependent isocitrate dehydrogenase, cytosolic
NADP-dependent isocitrate dehydrogenase, peroxisomal
NP_005887.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2q33.3

Summary Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]. lac of sum
Disorder MIM:

147700

Disorder Html:





The following IDH1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IDH1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu21156 NM_005896 Homo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$379
OHu21156 NM_001282387 Homo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$319
OHu21156 NM_001282386 Homo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$319

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu21156
Accession Version NM_005896.3
Sequence Information ORF Nucleotide Sequence (Length: 1245bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product isocitrate dehydrogenase [NADP] cytoplasmic
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA728868.1, BC021046.1 and N21575.1. This sequence is a reference standard in the RefSeqGene project. On Sep 5, 2013 this sequence version replaced gi:28178824. Summary: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]. Transcript Variant: This variant (1) and variants 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF020038.1, BC093020.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCCAAAA AAATCAGTGG CGGTTCTGTG GTAGAGATGC AAGGAGATGA AATGACACGA 
ATCATTTGGG AATTGATTAA AGAGAAACTC ATTTTTCCCT ACGTGGAATT GGATCTACAT
AGCTATGATT TAGGCATAGA GAATCGTGAT GCCACCAACG ACCAAGTCAC CAAGGATGCT
GCAGAAGCTA TAAAGAAGCA TAATGTTGGC GTCAAATGTG CCACTATCAC TCCTGATGAG
AAGAGGGTTG AGGAGTTCAA GTTGAAACAA ATGTGGAAAT CACCAAATGG CACCATACGA
AATATTCTGG GTGGCACGGT CTTCAGAGAA GCCATTATCT GCAAAAATAT CCCCCGGCTT
GTGAGTGGAT GGGTAAAACC TATCATCATA GGTCGTCATG CTTATGGGGA TCAATACAGA
GCAACTGATT TTGTTGTTCC TGGGCCTGGA AAAGTAGAGA TAACCTACAC ACCAAGTGAC
GGAACCCAAA AGGTGACATA CCTGGTACAT AACTTTGAAG AAGGTGGTGG TGTTGCCATG
GGGATGTATA ATCAAGATAA GTCAATTGAA GATTTTGCAC ACAGTTCCTT CCAAATGGCT
CTGTCTAAGG GTTGGCCTTT GTATCTGAGC ACCAAAAACA CTATTCTGAA GAAATATGAT
GGGCGTTTTA AAGACATCTT TCAGGAGATA TATGACAAGC AGTACAAGTC CCAGTTTGAA
GCTCAAAAGA TCTGGTATGA GCATAGGCTC ATCGACGACA TGGTGGCCCA AGCTATGAAA
TCAGAGGGAG GCTTCATCTG GGCCTGTAAA AACTATGATG GTGACGTGCA GTCGGACTCT
GTGGCCCAAG GGTATGGCTC TCTCGGCATG ATGACCAGCG TGCTGGTTTG TCCAGATGGC
AAGACAGTAG AAGCAGAGGC TGCCCACGGG ACTGTAACCC GTCACTACCG CATGTACCAG
AAAGGACAGG AGACGTCCAC CAATCCCATT GCTTCCATTT TTGCCTGGAC CAGAGGGTTA
GCCCACAGAG CAAAGCTTGA TAACAATAAA GAGCTTGCCT TCTTTGCAAA TGCTTTGGAA
GAAGTCTCTA TTGAGACAAT TGAGGCTGGC TTCATGACCA AGGACTTGGC TGCTTGCATT
AAAGGTTTAC CCAATGTGCA ACGTTCTGAC TACTTGAATA CATTTGAGTT CATGGATAAA
CTTGGAGAAA ACTTGAAGAT CAAACTAGCT CAGGCCAAAC TTTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_005887.2
CDS296..1540
Misc Feature(1)257..259(+)
Misc Feature(2)296..1528(+)
Misc Feature(3)299..301(+)
Misc Feature(4)575..595(+)
Misc Feature(5)710..712(+)
Misc Feature(6)929..931(+)
Misc Feature(7)1256..1258(+)
Exon (1)1..205
Gene:IDH1
Gene Synonym:
Exon (2)206..279
Gene:IDH1
Gene Synonym:
Exon (3)280..417
Gene:IDH1
Gene Synonym:
Exon (4)418..709
Gene:IDH1
Gene Synonym:
Exon (5)710..815
Gene:IDH1
Gene Synonym:
Exon (6)816..993
Gene:IDH1
Gene Synonym:
Exon (7)994..1145
Gene:IDH1
Gene Synonym:
Exon (8)1146..1286
Gene:IDH1
Gene Synonym:
Exon (9)1287..1449
Gene:IDH1
Gene Synonym:
Exon (10)1450..2392
Gene:IDH1
Gene Synonym:
Translation
Position Chain Variation Link
2 2 a, g dbSNP:183309933
31 31 a, g dbSNP:28384385
36 36 c, g dbSNP:558402258
55 55 a, g dbSNP:545303494
60 60 a, g dbSNP:753267741
151 151 a, g dbSNP:765770503
168 168 g, t dbSNP:199570676
189 189 a, g dbSNP:11554139
214 214 a, c dbSNP:144856461
242 242 g, t dbSNP:77588323
252 252 g, t dbSNP:11554136
257 257 c, t dbSNP:530522219
284 284 a, t dbSNP:756393190
289 289 a, c dbSNP:750612823
297 297 c, t dbSNP:767633282
298 298 a, g dbSNP:761830115
299 299 c, t dbSNP:751591193
305 305 a, c dbSNP:764523200
306 306 -, aaa dbSNP:757897398
308 308 -, a dbSNP:754375602
310 310 c, t dbSNP:763504710
311 311 a, g dbSNP:141118556
316 316 c, t dbSNP:756262551
317 317 a, g dbSNP:369048275
319 319 g, t dbSNP:759315203
339 339 a, g dbSNP:776449775
341 341 c, g dbSNP:192514135
350 350 a, c, g dbSNP:11554138
354 354 a, g, t dbSNP:201258988
364 364 c, g dbSNP:535551892
389 389 g, t dbSNP:142923780
392 392 c, t dbSNP:555882127
397 397 c, t dbSNP:753580953
398 398 a, g dbSNP:781398075
399 399 c, t dbSNP:757444051
404 404 c, t dbSNP:751649071
407 407 a, g dbSNP:374907791
420 420 a, g dbSNP:747226100
423 423 a, c dbSNP:777761062
440 440 c, t dbSNP:758524291
441 441 a, c, g dbSNP:765848107
454 454 c, t dbSNP:200305649
455 455 a, g dbSNP:754316078
479 479 a, g dbSNP:144593536
480 480 a, c dbSNP:760287259
492 492 a, g dbSNP:111834611
497 497 a, g dbSNP:772900658
506 506 a, g dbSNP:73070954
508 508 c, g dbSNP:372625136
522 522 c, t dbSNP:761344510
529 529 c, t dbSNP:773792199
534 534 a, t dbSNP:201790850
538 538 a, g dbSNP:368642313
540 540 a, g, t dbSNP:775186249
541 541 a, g dbSNP:575483212
542 542 g, t dbSNP:565065820
549 549 a, g dbSNP:778083499
559 559 g, t dbSNP:758622511
572 572 a, g dbSNP:748255418
577 577 a, g dbSNP:779126307
581 581 a, c dbSNP:755612585
589 589 c, t dbSNP:147684527
592 592 a, g dbSNP:142883642
593 593 a, c, t dbSNP:777129475
596 596 a, t dbSNP:762588599
604 604 a, g dbSNP:554273142
607 607 c, t dbSNP:761440376
610 610 c, t dbSNP:11554137
613 613 a, g dbSNP:763562621
614 614 a, g dbSNP:375658806
619 619 a, c dbSNP:199905024
623 623 a, g dbSNP:775517144
626 626 a, g dbSNP:769575550
629 629 a, g dbSNP:148542200
633 633 c, g, t dbSNP:772485166
644 644 a, g dbSNP:748380012
647 647 c, t dbSNP:371567806
649 649 c, t dbSNP:768836031
650 650 c, t dbSNP:747419787
651 651 a, g dbSNP:780642190
660 660 a, g dbSNP:756511163
661 661 c, t dbSNP:142572024
662 662 a, g dbSNP:781531255
668 668 c, g dbSNP:756942230
674 674 a, c dbSNP:751189315
679 679 c, t dbSNP:763685036
683 683 a, g dbSNP:762479277
689 689 a, c, t dbSNP:121913499
690 690 a, g dbSNP:121913500
700 700 c, t dbSNP:752078425
704 704 a, g dbSNP:765230533
730 730 c, t dbSNP:200875728
734 734 c, t dbSNP:766372640
739 739 a, g dbSNP:760579117
740 740 a, c dbSNP:773076468
741 741 c, t dbSNP:764425733
748 748 -, a dbSNP:776463533
751 751 a, g dbSNP:145324788
752 752 c, g dbSNP:765488909
763 763 c, t dbSNP:775678660
767 767 c, t dbSNP:769739538
775 775 c, t dbSNP:368904931
776 776 a, g dbSNP:148190775
792 792 c, t dbSNP:771358905
796 796 c, t dbSNP:747384833
817 817 a, c dbSNP:773611901
819 819 c, g dbSNP:772304188
820 820 c, t dbSNP:748499158
825 825 a, g dbSNP:149205273
827 827 a, g dbSNP:34218846
832 832 c, t dbSNP:748821411
833 833 a, g, t dbSNP:374474539
837 837 g, t dbSNP:750362869
841 841 c, g dbSNP:781132701
842 842 c, t dbSNP:757031960
843 843 a, g dbSNP:34599179
853 853 c, t dbSNP:781365220
860 860 a, g dbSNP:62193615
862 862 -, t dbSNP:778615377
875 875 c, t dbSNP:753981193
883 883 -, c dbSNP:756798008
888 888 a, g dbSNP:374340555
893 893 g, t dbSNP:370342586
898 898 g, t dbSNP:773617210
899 899 g, t dbSNP:772594297
903 903 a, g dbSNP:762089749
906 906 g, t dbSNP:774721532
915 915 g, t dbSNP:768250871
917 917 c, t dbSNP:587778402
918 918 a, g dbSNP:186787509
922 922 c, g dbSNP:182693596
936 936 c, g dbSNP:745329811
943 943 a, g dbSNP:377538429
954 954 a, g dbSNP:142801125
959 959 c, t dbSNP:751364381
960 960 a, g, t dbSNP:587778401
986 986 c, t dbSNP:755237893
992 992 a, t dbSNP:754042652
1001 1001 a, c dbSNP:746931121
1003 1003 a, g dbSNP:150747919
1006 1006 c, t dbSNP:758215808
1009 1009 g, t dbSNP:747900297
1021 1021 a, g dbSNP:377075054
1026 1026 g, t dbSNP:756272474
1031 1031 c, t dbSNP:373048968
1045 1045 c, t dbSNP:767630531
1048 1048 c, t dbSNP:139287550
1049 1049 a, g dbSNP:752130008
1051 1051 c, t dbSNP:764654685
1052 1052 a, g dbSNP:201394139
1061 1061 g, t dbSNP:775936968
1062 1062 c, t dbSNP:765037248
1083 1083 a, g dbSNP:759217866
1086 1086 c, g dbSNP:776041038
1091 1091 a, g dbSNP:199793362
1113 1113 a, g dbSNP:369039979
1115 1115 a, g dbSNP:773167891
1120 1120 c, t dbSNP:374900600
1121 1121 a, g dbSNP:747951792
1127 1127 c, t dbSNP:201822210
1128 1128 c, t dbSNP:756395116
1129 1129 a, g dbSNP:369424896
1130 1130 c, g dbSNP:781222279
1131 1131 a, g dbSNP:200372440
1134 1134 c, t dbSNP:757389021
1138 1138 a, g dbSNP:751525590
1147 1147 a, c, g dbSNP:755404974
1150 1150 a, t dbSNP:754088842
1167 1167 c, t dbSNP:141630472
1170 1170 a, c, t dbSNP:62193614
1171 1171 c, g dbSNP:750002102
1175 1175 a, g dbSNP:766985383
1178 1178 c, t dbSNP:370928521
1188 1188 c, t dbSNP:201477228
1189 1189 a, g dbSNP:188499120
1194 1194 a, g, t dbSNP:762820641
1198 1198 a, g dbSNP:775133036
1204 1204 a, g dbSNP:771247780
1206 1206 a, g dbSNP:567401796
1207 1207 a, g dbSNP:747198657
1212 1212 a, c dbSNP:138458978
1222 1222 c, t dbSNP:772297042
1223 1223 a, g dbSNP:773327968
1234 1234 c, t dbSNP:267599174
1236 1236 a, g dbSNP:779481497
1238 1238 c, g dbSNP:755385980
1241 1241 -, t dbSNP:767852664
1244 1244 c, t dbSNP:754290687
1248 1248 c, t dbSNP:377475055
1253 1253 c, t dbSNP:202210066
1254 1254 a, g dbSNP:199915613
1259 1259 a, g dbSNP:113732076
1270 1270 a, g dbSNP:148160119
1271 1271 c, t dbSNP:185564694
1275 1275 c, t dbSNP:767109945
1285 1285 c, t dbSNP:761315922
1298 1298 g, t dbSNP:763478345
1300 1300 c, t dbSNP:758405875
1309 1309 a, c dbSNP:752595957
1317 1317 a, c dbSNP:765179388
1332 1332 c, t dbSNP:759413310
1339 1339 c, g dbSNP:200718328
1347 1347 a, g dbSNP:776513405
1353 1353 a, c, g dbSNP:762126667
1355 1355 g, t dbSNP:536880332
1362 1362 c, t dbSNP:768596300
1367 1367 -, g dbSNP:766547920
1370 1370 c, t dbSNP:762867621
1371 1371 a, t dbSNP:775893896
1382 1382 g, t dbSNP:770226824
1385 1385 a, g dbSNP:372711897
1389 1389 a, t dbSNP:781533021
1390 1390 a, g dbSNP:756940135
1393 1393 a, g dbSNP:746615557
1394 1394 a, g dbSNP:777431979
1395 1395 c, t dbSNP:200591678
1401 1401 c, t dbSNP:752187592
1410 1410 c, t dbSNP:765282142
1413 1413 a, c dbSNP:754957038
1414 1414 c, t dbSNP:369234651
1415 1415 a, g dbSNP:201267625
1424 1424 g, t dbSNP:775128585
1428 1428 c, t dbSNP:766246232
1436 1436 a, t dbSNP:762143982
1438 1438 -, a dbSNP:763043045
1444 1444 a, c dbSNP:141895800
1446 1446 c, t dbSNP:764338499
1447 1447 c, t dbSNP:762954264
1448 1448 a, g dbSNP:775623666
1450 1450 -, ttacccaat dbSNP:771971686
1450 1450 c, t dbSNP:760963029
1458 1458 a, g dbSNP:566072712
1477 1477 a, g dbSNP:771692756
1490 1490 c, g dbSNP:747809723
1491 1491 a, g dbSNP:778615461
1497 1497 c, t dbSNP:768304856
1538 1538 -, t dbSNP:759303223
1540 1540 a, c dbSNP:3778
1546 1546 c, t dbSNP:748768472
1553 1553 c, g dbSNP:780146398
1554 1554 c, t dbSNP:114939378
1555 1555 a, t dbSNP:750251172
1565 1565 a, g dbSNP:781009655
1586 1586 a, t dbSNP:758597026
1590 1590 c, g dbSNP:200122564
1610 1610 c, t dbSNP:768957576
1619 1619 -, a dbSNP:532947500
1647 1647 g, t dbSNP:185188955
1656 1656 a, g dbSNP:112722672
1676 1676 a, c dbSNP:749424817
1694 1694 c, t dbSNP:78539882
1699 1699 c, t dbSNP:533578934
1760 1760 -, atttt dbSNP:781093485
1834 1834 a, t dbSNP:564140008
1835 1835 c, t dbSNP:369898908
1876 1876 a, g dbSNP:1044416
1934 1934 c, g dbSNP:561722525
1964 1964 c, g dbSNP:746228832
2031 2031 a, t dbSNP:13026
2032 2032 g, t dbSNP:6730955
2122 2122 a, g dbSNP:150906753
2140 2140 c, t dbSNP:575807296
2141 2141 a, g dbSNP:180928167
2152 2152 g, t dbSNP:557950423
2180 2180 c, g dbSNP:16841606
2212 2212 a, t dbSNP:747993945
2216 2216 a, g dbSNP:576632204
2244 2244 c, t dbSNP:557788219
2247 2247 c, t dbSNP:15344
2278 2278 a, c dbSNP:369225330

Target ORF information:

RefSeq Version NM_005896
Organism Homo sapiens (human)
Definition Homo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005896

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCCAAAA AAATCAGTGG CGGTTCTGTG GTAGAGATGC AAGGAGATGA AATGACACGA 
ATCATTTGGG AATTGATTAA AGAGAAACTC ATTTTTCCCT ACGTGGAATT GGATCTACAT
AGCTATGATT TAGGCATAGA GAATCGTGAT GCCACCAACG ACCAAGTCAC CAAGGATGCT
GCAGAAGCTA TAAAGAAGCA TAATGTTGGC GTCAAATGTG CCACTATCAC TCCTGATGAG
AAGAGGGTTG AGGAGTTCAA GTTGAAACAA ATGTGGAAAT CACCAAATGG CACCATACGA
AATATTCTGG GTGGCACGGT CTTCAGAGAA GCCATTATCT GCAAAAATAT CCCCCGGCTT
GTGAGTGGAT GGGTAAAACC TATCATCATA GGTCGTCATG CTTATGGGGA TCAATACAGA
GCAACTGATT TTGTTGTTCC TGGGCCTGGA AAAGTAGAGA TAACCTACAC ACCAAGTGAC
GGAACCCAAA AGGTGACATA CCTGGTACAT AACTTTGAAG AAGGTGGTGG TGTTGCCATG
GGGATGTATA ATCAAGATAA GTCAATTGAA GATTTTGCAC ACAGTTCCTT CCAAATGGCT
CTGTCTAAGG GTTGGCCTTT GTATCTGAGC ACCAAAAACA CTATTCTGAA GAAATATGAT
GGGCGTTTTA AAGACATCTT TCAGGAGATA TATGACAAGC AGTACAAGTC CCAGTTTGAA
GCTCAAAAGA TCTGGTATGA GCATAGGCTC ATCGACGACA TGGTGGCCCA AGCTATGAAA
TCAGAGGGAG GCTTCATCTG GGCCTGTAAA AACTATGATG GTGACGTGCA GTCGGACTCT
GTGGCCCAAG GGTATGGCTC TCTCGGCATG ATGACCAGCG TGCTGGTTTG TCCAGATGGC
AAGACAGTAG AAGCAGAGGC TGCCCACGGG ACTGTAACCC GTCACTACCG CATGTACCAG
AAAGGACAGG AGACGTCCAC CAATCCCATT GCTTCCATTT TTGCCTGGAC CAGAGGGTTA
GCCCACAGAG CAAAGCTTGA TAACAATAAA GAGCTTGCCT TCTTTGCAAA TGCTTTGGAA
GAAGTCTCTA TTGAGACAAT TGAGGCTGGC TTCATGACCA AGGACTTGGC TGCTTGCATT
AAAGGTTTAC CCAATGTGCA ACGTTCTGAC TACTTGAATA CATTTGAGTT CATGGATAAA
CTTGGAGAAA ACTTGAAGAT CAAACTAGCT CAGGCCAAAC TTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21156
Accession Version NM_001282387.1
Sequence Information ORF Nucleotide Sequence (Length: 1245bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product isocitrate dehydrogenase [NADP] cytoplasmic
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR158630.1, BC012846.1 and N21575.1. This sequence is a reference standard in the RefSeqGene project. On Sep 5, 2013 this sequence version replaced gi:530370286. Summary: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]. Transcript Variant: This variant (3) has an alternate 5' UTR exon, compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC012846.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCCAAAA AAATCAGTGG CGGTTCTGTG GTAGAGATGC AAGGAGATGA AATGACACGA 
ATCATTTGGG AATTGATTAA AGAGAAACTC ATTTTTCCCT ACGTGGAATT GGATCTACAT
AGCTATGATT TAGGCATAGA GAATCGTGAT GCCACCAACG ACCAAGTCAC CAAGGATGCT
GCAGAAGCTA TAAAGAAGCA TAATGTTGGC GTCAAATGTG CCACTATCAC TCCTGATGAG
AAGAGGGTTG AGGAGTTCAA GTTGAAACAA ATGTGGAAAT CACCAAATGG CACCATACGA
AATATTCTGG GTGGCACGGT CTTCAGAGAA GCCATTATCT GCAAAAATAT CCCCCGGCTT
GTGAGTGGAT GGGTAAAACC TATCATCATA GGTCGTCATG CTTATGGGGA TCAATACAGA
GCAACTGATT TTGTTGTTCC TGGGCCTGGA AAAGTAGAGA TAACCTACAC ACCAAGTGAC
GGAACCCAAA AGGTGACATA CCTGGTACAT AACTTTGAAG AAGGTGGTGG TGTTGCCATG
GGGATGTATA ATCAAGATAA GTCAATTGAA GATTTTGCAC ACAGTTCCTT CCAAATGGCT
CTGTCTAAGG GTTGGCCTTT GTATCTGAGC ACCAAAAACA CTATTCTGAA GAAATATGAT
GGGCGTTTTA AAGACATCTT TCAGGAGATA TATGACAAGC AGTACAAGTC CCAGTTTGAA
GCTCAAAAGA TCTGGTATGA GCATAGGCTC ATCGACGACA TGGTGGCCCA AGCTATGAAA
TCAGAGGGAG GCTTCATCTG GGCCTGTAAA AACTATGATG GTGACGTGCA GTCGGACTCT
GTGGCCCAAG GGTATGGCTC TCTCGGCATG ATGACCAGCG TGCTGGTTTG TCCAGATGGC
AAGACAGTAG AAGCAGAGGC TGCCCACGGG ACTGTAACCC GTCACTACCG CATGTACCAG
AAAGGACAGG AGACGTCCAC CAATCCCATT GCTTCCATTT TTGCCTGGAC CAGAGGGTTA
GCCCACAGAG CAAAGCTTGA TAACAATAAA GAGCTTGCCT TCTTTGCAAA TGCTTTGGAA
GAAGTCTCTA TTGAGACAAT TGAGGCTGGC TTCATGACCA AGGACTTGGC TGCTTGCATT
AAAGGTTTAC CCAATGTGCA ACGTTCTGAC TACTTGAATA CATTTGAGTT CATGGATAAA
CTTGGAGAAA ACTTGAAGAT CAAACTAGCT CAGGCCAAAC TTTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001269316.1
CDS364..1608
Misc Feature(1)325..327(+)
Misc Feature(2)364..1596(+)
Misc Feature(3)367..369(+)
Misc Feature(4)643..663(+)
Misc Feature(5)778..780(+)
Misc Feature(6)997..999(+)
Misc Feature(7)1324..1326(+)
Exon (1)1..273
Gene:IDH1
Gene Synonym:
Exon (2)274..347
Gene:IDH1
Gene Synonym:
Exon (3)348..485
Gene:IDH1
Gene Synonym:
Exon (4)486..777
Gene:IDH1
Gene Synonym:
Exon (5)778..883
Gene:IDH1
Gene Synonym:
Exon (6)884..1061
Gene:IDH1
Gene Synonym:
Exon (7)1062..1213
Gene:IDH1
Gene Synonym:
Exon (8)1214..1354
Gene:IDH1
Gene Synonym:
Exon (9)1355..1517
Gene:IDH1
Gene Synonym:
Exon (10)1518..2460
Gene:IDH1
Gene Synonym:
Translation
Position Chain Variation Link
5 5 -, a dbSNP:35585619
33 33 c, t dbSNP:189891775
75 75 c, t dbSNP:538360925
93 93 a, g dbSNP:563367302
105 105 a, g dbSNP:543687633
108 108 c, t dbSNP:538695310
116 116 c, t dbSNP:79062167
179 179 a, g dbSNP:547002679
191 191 c, t dbSNP:771102147
245 245 -, t dbSNP:149743342
282 282 a, c dbSNP:144856461
310 310 g, t dbSNP:77588323
320 320 g, t dbSNP:11554136
325 325 c, t dbSNP:530522219
352 352 a, t dbSNP:756393190
357 357 a, c dbSNP:750612823
365 365 c, t dbSNP:767633282
366 366 a, g dbSNP:761830115
367 367 c, t dbSNP:751591193
373 373 a, c dbSNP:764523200
374 374 -, aaa dbSNP:757897398
376 376 -, a dbSNP:754375602
378 378 c, t dbSNP:763504710
379 379 a, g dbSNP:141118556
384 384 c, t dbSNP:756262551
385 385 a, g dbSNP:369048275
387 387 g, t dbSNP:759315203
407 407 a, g dbSNP:776449775
409 409 c, g dbSNP:192514135
418 418 a, c, g dbSNP:11554138
422 422 a, g, t dbSNP:201258988
432 432 c, g dbSNP:535551892
457 457 g, t dbSNP:142923780
460 460 c, t dbSNP:555882127
465 465 c, t dbSNP:753580953
466 466 a, g dbSNP:781398075
467 467 c, t dbSNP:757444051
472 472 c, t dbSNP:751649071
475 475 a, g dbSNP:374907791
488 488 a, g dbSNP:747226100
491 491 a, c dbSNP:777761062
508 508 c, t dbSNP:758524291
509 509 a, c, g dbSNP:765848107
522 522 c, t dbSNP:200305649
523 523 a, g dbSNP:754316078
547 547 a, g dbSNP:144593536
548 548 a, c dbSNP:760287259
560 560 a, g dbSNP:111834611
565 565 a, g dbSNP:772900658
574 574 a, g dbSNP:73070954
576 576 c, g dbSNP:372625136
590 590 c, t dbSNP:761344510
597 597 c, t dbSNP:773792199
602 602 a, t dbSNP:201790850
606 606 a, g dbSNP:368642313
608 608 a, g, t dbSNP:775186249
609 609 a, g dbSNP:575483212
610 610 g, t dbSNP:565065820
617 617 a, g dbSNP:778083499
627 627 g, t dbSNP:758622511
640 640 a, g dbSNP:748255418
645 645 a, g dbSNP:779126307
649 649 a, c dbSNP:755612585
657 657 c, t dbSNP:147684527
660 660 a, g dbSNP:142883642
661 661 a, c, t dbSNP:777129475
664 664 a, t dbSNP:762588599
672 672 a, g dbSNP:554273142
675 675 c, t dbSNP:761440376
678 678 c, t dbSNP:11554137
681 681 a, g dbSNP:763562621
682 682 a, g dbSNP:375658806
687 687 a, c dbSNP:199905024
691 691 a, g dbSNP:775517144
694 694 a, g dbSNP:769575550
697 697 a, g dbSNP:148542200
701 701 c, g, t dbSNP:772485166
712 712 a, g dbSNP:748380012
715 715 c, t dbSNP:371567806
717 717 c, t dbSNP:768836031
718 718 c, t dbSNP:747419787
719 719 a, g dbSNP:780642190
728 728 a, g dbSNP:756511163
729 729 c, t dbSNP:142572024
730 730 a, g dbSNP:781531255
736 736 c, g dbSNP:756942230
742 742 a, c dbSNP:751189315
747 747 c, t dbSNP:763685036
751 751 a, g dbSNP:762479277
757 757 a, c, t dbSNP:121913499
758 758 a, g dbSNP:121913500
768 768 c, t dbSNP:752078425
772 772 a, g dbSNP:765230533
798 798 c, t dbSNP:200875728
802 802 c, t dbSNP:766372640
807 807 a, g dbSNP:760579117
808 808 a, c dbSNP:773076468
809 809 c, t dbSNP:764425733
816 816 -, a dbSNP:776463533
819 819 a, g dbSNP:145324788
820 820 c, g dbSNP:765488909
831 831 c, t dbSNP:775678660
835 835 c, t dbSNP:769739538
843 843 c, t dbSNP:368904931
844 844 a, g dbSNP:148190775
860 860 c, t dbSNP:771358905
864 864 c, t dbSNP:747384833
885 885 a, c dbSNP:773611901
887 887 c, g dbSNP:772304188
888 888 c, t dbSNP:748499158
893 893 a, g dbSNP:149205273
895 895 a, g dbSNP:34218846
900 900 c, t dbSNP:748821411
901 901 a, g, t dbSNP:374474539
905 905 g, t dbSNP:750362869
909 909 c, g dbSNP:781132701
910 910 c, t dbSNP:757031960
911 911 a, g dbSNP:34599179
921 921 c, t dbSNP:781365220
928 928 a, g dbSNP:62193615
930 930 -, t dbSNP:778615377
943 943 c, t dbSNP:753981193
951 951 -, c dbSNP:756798008
956 956 a, g dbSNP:374340555
961 961 g, t dbSNP:370342586
966 966 g, t dbSNP:773617210
967 967 g, t dbSNP:772594297
971 971 a, g dbSNP:762089749
974 974 g, t dbSNP:774721532
983 983 g, t dbSNP:768250871
985 985 c, t dbSNP:587778402
986 986 a, g dbSNP:186787509
990 990 c, g dbSNP:182693596
1004 1004 c, g dbSNP:745329811
1011 1011 a, g dbSNP:377538429
1022 1022 a, g dbSNP:142801125
1027 1027 c, t dbSNP:751364381
1028 1028 a, g, t dbSNP:587778401
1054 1054 c, t dbSNP:755237893
1060 1060 a, t dbSNP:754042652
1069 1069 a, c dbSNP:746931121
1071 1071 a, g dbSNP:150747919
1074 1074 c, t dbSNP:758215808
1077 1077 g, t dbSNP:747900297
1089 1089 a, g dbSNP:377075054
1094 1094 g, t dbSNP:756272474
1099 1099 c, t dbSNP:373048968
1113 1113 c, t dbSNP:767630531
1116 1116 c, t dbSNP:139287550
1117 1117 a, g dbSNP:752130008
1119 1119 c, t dbSNP:764654685
1120 1120 a, g dbSNP:201394139
1129 1129 g, t dbSNP:775936968
1130 1130 c, t dbSNP:765037248
1151 1151 a, g dbSNP:759217866
1154 1154 c, g dbSNP:776041038
1159 1159 a, g dbSNP:199793362
1181 1181 a, g dbSNP:369039979
1183 1183 a, g dbSNP:773167891
1188 1188 c, t dbSNP:374900600
1189 1189 a, g dbSNP:747951792
1195 1195 c, t dbSNP:201822210
1196 1196 c, t dbSNP:756395116
1197 1197 a, g dbSNP:369424896
1198 1198 c, g dbSNP:781222279
1199 1199 a, g dbSNP:200372440
1202 1202 c, t dbSNP:757389021
1206 1206 a, g dbSNP:751525590
1215 1215 a, c, g dbSNP:755404974
1218 1218 a, t dbSNP:754088842
1235 1235 c, t dbSNP:141630472
1238 1238 a, c, t dbSNP:62193614
1239 1239 c, g dbSNP:750002102
1243 1243 a, g dbSNP:766985383
1246 1246 c, t dbSNP:370928521
1256 1256 c, t dbSNP:201477228
1257 1257 a, g dbSNP:188499120
1262 1262 a, g, t dbSNP:762820641
1266 1266 a, g dbSNP:775133036
1272 1272 a, g dbSNP:771247780
1274 1274 a, g dbSNP:567401796
1275 1275 a, g dbSNP:747198657
1280 1280 a, c dbSNP:138458978
1290 1290 c, t dbSNP:772297042
1291 1291 a, g dbSNP:773327968
1302 1302 c, t dbSNP:267599174
1304 1304 a, g dbSNP:779481497
1306 1306 c, g dbSNP:755385980
1309 1309 -, t dbSNP:767852664
1312 1312 c, t dbSNP:754290687
1316 1316 c, t dbSNP:377475055
1321 1321 c, t dbSNP:202210066
1322 1322 a, g dbSNP:199915613
1327 1327 a, g dbSNP:113732076
1338 1338 a, g dbSNP:148160119
1339 1339 c, t dbSNP:185564694
1343 1343 c, t dbSNP:767109945
1353 1353 c, t dbSNP:761315922
1366 1366 g, t dbSNP:763478345
1368 1368 c, t dbSNP:758405875
1377 1377 a, c dbSNP:752595957
1385 1385 a, c dbSNP:765179388
1400 1400 c, t dbSNP:759413310
1407 1407 c, g dbSNP:200718328
1415 1415 a, g dbSNP:776513405
1421 1421 a, c, g dbSNP:762126667
1423 1423 g, t dbSNP:536880332
1430 1430 c, t dbSNP:768596300
1435 1435 -, g dbSNP:766547920
1438 1438 c, t dbSNP:762867621
1439 1439 a, t dbSNP:775893896
1450 1450 g, t dbSNP:770226824
1453 1453 a, g dbSNP:372711897
1457 1457 a, t dbSNP:781533021
1458 1458 a, g dbSNP:756940135
1461 1461 a, g dbSNP:746615557
1462 1462 a, g dbSNP:777431979
1463 1463 c, t dbSNP:200591678
1469 1469 c, t dbSNP:752187592
1478 1478 c, t dbSNP:765282142
1481 1481 a, c dbSNP:754957038
1482 1482 c, t dbSNP:369234651
1483 1483 a, g dbSNP:201267625
1492 1492 g, t dbSNP:775128585
1496 1496 c, t dbSNP:766246232
1504 1504 a, t dbSNP:762143982
1506 1506 -, a dbSNP:763043045
1512 1512 a, c dbSNP:141895800
1514 1514 c, t dbSNP:764338499
1515 1515 c, t dbSNP:762954264
1516 1516 a, g dbSNP:775623666
1518 1518 -, ttacccaat dbSNP:771971686
1518 1518 c, t dbSNP:760963029
1526 1526 a, g dbSNP:566072712
1545 1545 a, g dbSNP:771692756
1558 1558 c, g dbSNP:747809723
1559 1559 a, g dbSNP:778615461
1565 1565 c, t dbSNP:768304856
1606 1606 -, t dbSNP:759303223
1608 1608 a, c dbSNP:3778
1614 1614 c, t dbSNP:748768472
1621 1621 c, g dbSNP:780146398
1622 1622 c, t dbSNP:114939378
1623 1623 a, t dbSNP:750251172
1633 1633 a, g dbSNP:781009655
1654 1654 a, t dbSNP:758597026
1658 1658 c, g dbSNP:200122564
1678 1678 c, t dbSNP:768957576
1687 1687 -, a dbSNP:532947500
1715 1715 g, t dbSNP:185188955
1724 1724 a, g dbSNP:112722672
1744 1744 a, c dbSNP:749424817
1762 1762 c, t dbSNP:78539882
1767 1767 c, t dbSNP:533578934
1828 1828 -, atttt dbSNP:781093485
1902 1902 a, t dbSNP:564140008
1903 1903 c, t dbSNP:369898908
1944 1944 a, g dbSNP:1044416
2002 2002 c, g dbSNP:561722525
2032 2032 c, g dbSNP:746228832
2099 2099 a, t dbSNP:13026
2100 2100 g, t dbSNP:6730955
2190 2190 a, g dbSNP:150906753
2208 2208 c, t dbSNP:575807296
2209 2209 a, g dbSNP:180928167
2220 2220 g, t dbSNP:557950423
2248 2248 c, g dbSNP:16841606
2280 2280 a, t dbSNP:747993945
2284 2284 a, g dbSNP:576632204
2312 2312 c, t dbSNP:557788219
2315 2315 c, t dbSNP:15344
2346 2346 a, c dbSNP:369225330

Target ORF information:

RefSeq Version NM_001282387
Organism Homo sapiens (human)
Definition Homo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282387

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCCAAAA AAATCAGTGG CGGTTCTGTG GTAGAGATGC AAGGAGATGA AATGACACGA 
ATCATTTGGG AATTGATTAA AGAGAAACTC ATTTTTCCCT ACGTGGAATT GGATCTACAT
AGCTATGATT TAGGCATAGA GAATCGTGAT GCCACCAACG ACCAAGTCAC CAAGGATGCT
GCAGAAGCTA TAAAGAAGCA TAATGTTGGC GTCAAATGTG CCACTATCAC TCCTGATGAG
AAGAGGGTTG AGGAGTTCAA GTTGAAACAA ATGTGGAAAT CACCAAATGG CACCATACGA
AATATTCTGG GTGGCACGGT CTTCAGAGAA GCCATTATCT GCAAAAATAT CCCCCGGCTT
GTGAGTGGAT GGGTAAAACC TATCATCATA GGTCGTCATG CTTATGGGGA TCAATACAGA
GCAACTGATT TTGTTGTTCC TGGGCCTGGA AAAGTAGAGA TAACCTACAC ACCAAGTGAC
GGAACCCAAA AGGTGACATA CCTGGTACAT AACTTTGAAG AAGGTGGTGG TGTTGCCATG
GGGATGTATA ATCAAGATAA GTCAATTGAA GATTTTGCAC ACAGTTCCTT CCAAATGGCT
CTGTCTAAGG GTTGGCCTTT GTATCTGAGC ACCAAAAACA CTATTCTGAA GAAATATGAT
GGGCGTTTTA AAGACATCTT TCAGGAGATA TATGACAAGC AGTACAAGTC CCAGTTTGAA
GCTCAAAAGA TCTGGTATGA GCATAGGCTC ATCGACGACA TGGTGGCCCA AGCTATGAAA
TCAGAGGGAG GCTTCATCTG GGCCTGTAAA AACTATGATG GTGACGTGCA GTCGGACTCT
GTGGCCCAAG GGTATGGCTC TCTCGGCATG ATGACCAGCG TGCTGGTTTG TCCAGATGGC
AAGACAGTAG AAGCAGAGGC TGCCCACGGG ACTGTAACCC GTCACTACCG CATGTACCAG
AAAGGACAGG AGACGTCCAC CAATCCCATT GCTTCCATTT TTGCCTGGAC CAGAGGGTTA
GCCCACAGAG CAAAGCTTGA TAACAATAAA GAGCTTGCCT TCTTTGCAAA TGCTTTGGAA
GAAGTCTCTA TTGAGACAAT TGAGGCTGGC TTCATGACCA AGGACTTGGC TGCTTGCATT
AAAGGTTTAC CCAATGTGCA ACGTTCTGAC TACTTGAATA CATTTGAGTT CATGGATAAA
CTTGGAGAAA ACTTGAAGAT CAAACTAGCT CAGGCCAAAC TTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21156
Accession Version NM_001282386.1
Sequence Information ORF Nucleotide Sequence (Length: 1245bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product isocitrate dehydrogenase [NADP] cytoplasmic
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA707595.1, BC021046.1 and N21575.1. This sequence is a reference standard in the RefSeqGene project. On Sep 5, 2013 this sequence version replaced gi:530370284. Summary: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]. Transcript Variant: This variant (2) has an alternate 5' UTR exon, compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX537411.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCCAAAA AAATCAGTGG CGGTTCTGTG GTAGAGATGC AAGGAGATGA AATGACACGA 
ATCATTTGGG AATTGATTAA AGAGAAACTC ATTTTTCCCT ACGTGGAATT GGATCTACAT
AGCTATGATT TAGGCATAGA GAATCGTGAT GCCACCAACG ACCAAGTCAC CAAGGATGCT
GCAGAAGCTA TAAAGAAGCA TAATGTTGGC GTCAAATGTG CCACTATCAC TCCTGATGAG
AAGAGGGTTG AGGAGTTCAA GTTGAAACAA ATGTGGAAAT CACCAAATGG CACCATACGA
AATATTCTGG GTGGCACGGT CTTCAGAGAA GCCATTATCT GCAAAAATAT CCCCCGGCTT
GTGAGTGGAT GGGTAAAACC TATCATCATA GGTCGTCATG CTTATGGGGA TCAATACAGA
GCAACTGATT TTGTTGTTCC TGGGCCTGGA AAAGTAGAGA TAACCTACAC ACCAAGTGAC
GGAACCCAAA AGGTGACATA CCTGGTACAT AACTTTGAAG AAGGTGGTGG TGTTGCCATG
GGGATGTATA ATCAAGATAA GTCAATTGAA GATTTTGCAC ACAGTTCCTT CCAAATGGCT
CTGTCTAAGG GTTGGCCTTT GTATCTGAGC ACCAAAAACA CTATTCTGAA GAAATATGAT
GGGCGTTTTA AAGACATCTT TCAGGAGATA TATGACAAGC AGTACAAGTC CCAGTTTGAA
GCTCAAAAGA TCTGGTATGA GCATAGGCTC ATCGACGACA TGGTGGCCCA AGCTATGAAA
TCAGAGGGAG GCTTCATCTG GGCCTGTAAA AACTATGATG GTGACGTGCA GTCGGACTCT
GTGGCCCAAG GGTATGGCTC TCTCGGCATG ATGACCAGCG TGCTGGTTTG TCCAGATGGC
AAGACAGTAG AAGCAGAGGC TGCCCACGGG ACTGTAACCC GTCACTACCG CATGTACCAG
AAAGGACAGG AGACGTCCAC CAATCCCATT GCTTCCATTT TTGCCTGGAC CAGAGGGTTA
GCCCACAGAG CAAAGCTTGA TAACAATAAA GAGCTTGCCT TCTTTGCAAA TGCTTTGGAA
GAAGTCTCTA TTGAGACAAT TGAGGCTGGC TTCATGACCA AGGACTTGGC TGCTTGCATT
AAAGGTTTAC CCAATGTGCA ACGTTCTGAC TACTTGAATA CATTTGAGTT CATGGATAAA
CTTGGAGAAA ACTTGAAGAT CAAACTAGCT CAGGCCAAAC TTTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001269315.1
CDS207..1451
Misc Feature(1)168..170(+)
Misc Feature(2)207..1439(+)
Misc Feature(3)210..212(+)
Misc Feature(4)486..506(+)
Misc Feature(5)621..623(+)
Misc Feature(6)840..842(+)
Misc Feature(7)1167..1169(+)
Exon (1)1..116
Gene:IDH1
Gene Synonym:
Exon (2)117..190
Gene:IDH1
Gene Synonym:
Exon (3)191..328
Gene:IDH1
Gene Synonym:
Exon (4)329..620
Gene:IDH1
Gene Synonym:
Exon (5)621..726
Gene:IDH1
Gene Synonym:
Exon (6)727..904
Gene:IDH1
Gene Synonym:
Exon (7)905..1056
Gene:IDH1
Gene Synonym:
Exon (8)1057..1197
Gene:IDH1
Gene Synonym:
Exon (9)1198..1360
Gene:IDH1
Gene Synonym:
Exon (10)1361..2303
Gene:IDH1
Gene Synonym:
Translation
Position Chain Variation Link
52 52 a, c dbSNP:560626062
56 56 a, c dbSNP:550282579
59 59 a, g dbSNP:147924092
66 66 c, g dbSNP:183195427
81 81 a, c dbSNP:569800762
82 82 -, c dbSNP:397873786
86 86 -, c dbSNP:35374815
108 108 g, t dbSNP:544927674
114 114 a, t dbSNP:377011618
125 125 a, c dbSNP:144856461
153 153 g, t dbSNP:77588323
163 163 g, t dbSNP:11554136
168 168 c, t dbSNP:530522219
195 195 a, t dbSNP:756393190
200 200 a, c dbSNP:750612823
208 208 c, t dbSNP:767633282
209 209 a, g dbSNP:761830115
210 210 c, t dbSNP:751591193
216 216 a, c dbSNP:764523200
217 217 -, aaa dbSNP:757897398
219 219 -, a dbSNP:754375602
221 221 c, t dbSNP:763504710
222 222 a, g dbSNP:141118556
227 227 c, t dbSNP:756262551
228 228 a, g dbSNP:369048275
230 230 g, t dbSNP:759315203
250 250 a, g dbSNP:776449775
252 252 c, g dbSNP:192514135
261 261 a, c, g dbSNP:11554138
265 265 a, g, t dbSNP:201258988
275 275 c, g dbSNP:535551892
300 300 g, t dbSNP:142923780
303 303 c, t dbSNP:555882127
308 308 c, t dbSNP:753580953
309 309 a, g dbSNP:781398075
310 310 c, t dbSNP:757444051
315 315 c, t dbSNP:751649071
318 318 a, g dbSNP:374907791
331 331 a, g dbSNP:747226100
334 334 a, c dbSNP:777761062
351 351 c, t dbSNP:758524291
352 352 a, c, g dbSNP:765848107
365 365 c, t dbSNP:200305649
366 366 a, g dbSNP:754316078
390 390 a, g dbSNP:144593536
391 391 a, c dbSNP:760287259
403 403 a, g dbSNP:111834611
408 408 a, g dbSNP:772900658
417 417 a, g dbSNP:73070954
419 419 c, g dbSNP:372625136
433 433 c, t dbSNP:761344510
440 440 c, t dbSNP:773792199
445 445 a, t dbSNP:201790850
449 449 a, g dbSNP:368642313
451 451 a, g, t dbSNP:775186249
452 452 a, g dbSNP:575483212
453 453 g, t dbSNP:565065820
460 460 a, g dbSNP:778083499
470 470 g, t dbSNP:758622511
483 483 a, g dbSNP:748255418
488 488 a, g dbSNP:779126307
492 492 a, c dbSNP:755612585
500 500 c, t dbSNP:147684527
503 503 a, g dbSNP:142883642
504 504 a, c, t dbSNP:777129475
507 507 a, t dbSNP:762588599
515 515 a, g dbSNP:554273142
518 518 c, t dbSNP:761440376
521 521 c, t dbSNP:11554137
524 524 a, g dbSNP:763562621
525 525 a, g dbSNP:375658806
530 530 a, c dbSNP:199905024
534 534 a, g dbSNP:775517144
537 537 a, g dbSNP:769575550
540 540 a, g dbSNP:148542200
544 544 c, g, t dbSNP:772485166
555 555 a, g dbSNP:748380012
558 558 c, t dbSNP:371567806
560 560 c, t dbSNP:768836031
561 561 c, t dbSNP:747419787
562 562 a, g dbSNP:780642190
571 571 a, g dbSNP:756511163
572 572 c, t dbSNP:142572024
573 573 a, g dbSNP:781531255
579 579 c, g dbSNP:756942230
585 585 a, c dbSNP:751189315
590 590 c, t dbSNP:763685036
594 594 a, g dbSNP:762479277
600 600 a, c, t dbSNP:121913499
601 601 a, g dbSNP:121913500
611 611 c, t dbSNP:752078425
615 615 a, g dbSNP:765230533
641 641 c, t dbSNP:200875728
645 645 c, t dbSNP:766372640
650 650 a, g dbSNP:760579117
651 651 a, c dbSNP:773076468
652 652 c, t dbSNP:764425733
659 659 -, a dbSNP:776463533
662 662 a, g dbSNP:145324788
663 663 c, g dbSNP:765488909
674 674 c, t dbSNP:775678660
678 678 c, t dbSNP:769739538
686 686 c, t dbSNP:368904931
687 687 a, g dbSNP:148190775
703 703 c, t dbSNP:771358905
707 707 c, t dbSNP:747384833
728 728 a, c dbSNP:773611901
730 730 c, g dbSNP:772304188
731 731 c, t dbSNP:748499158
736 736 a, g dbSNP:149205273
738 738 a, g dbSNP:34218846
743 743 c, t dbSNP:748821411
744 744 a, g, t dbSNP:374474539
748 748 g, t dbSNP:750362869
752 752 c, g dbSNP:781132701
753 753 c, t dbSNP:757031960
754 754 a, g dbSNP:34599179
764 764 c, t dbSNP:781365220
771 771 a, g dbSNP:62193615
773 773 -, t dbSNP:778615377
786 786 c, t dbSNP:753981193
794 794 -, c dbSNP:756798008
799 799 a, g dbSNP:374340555
804 804 g, t dbSNP:370342586
809 809 g, t dbSNP:773617210
810 810 g, t dbSNP:772594297
814 814 a, g dbSNP:762089749
817 817 g, t dbSNP:774721532
826 826 g, t dbSNP:768250871
828 828 c, t dbSNP:587778402
829 829 a, g dbSNP:186787509
833 833 c, g dbSNP:182693596
847 847 c, g dbSNP:745329811
854 854 a, g dbSNP:377538429
865 865 a, g dbSNP:142801125
870 870 c, t dbSNP:751364381
871 871 a, g, t dbSNP:587778401
897 897 c, t dbSNP:755237893
903 903 a, t dbSNP:754042652
912 912 a, c dbSNP:746931121
914 914 a, g dbSNP:150747919
917 917 c, t dbSNP:758215808
920 920 g, t dbSNP:747900297
932 932 a, g dbSNP:377075054
937 937 g, t dbSNP:756272474
942 942 c, t dbSNP:373048968
956 956 c, t dbSNP:767630531
959 959 c, t dbSNP:139287550
960 960 a, g dbSNP:752130008
962 962 c, t dbSNP:764654685
963 963 a, g dbSNP:201394139
972 972 g, t dbSNP:775936968
973 973 c, t dbSNP:765037248
994 994 a, g dbSNP:759217866
997 997 c, g dbSNP:776041038
1002 1002 a, g dbSNP:199793362
1024 1024 a, g dbSNP:369039979
1026 1026 a, g dbSNP:773167891
1031 1031 c, t dbSNP:374900600
1032 1032 a, g dbSNP:747951792
1038 1038 c, t dbSNP:201822210
1039 1039 c, t dbSNP:756395116
1040 1040 a, g dbSNP:369424896
1041 1041 c, g dbSNP:781222279
1042 1042 a, g dbSNP:200372440
1045 1045 c, t dbSNP:757389021
1049 1049 a, g dbSNP:751525590
1058 1058 a, c, g dbSNP:755404974
1061 1061 a, t dbSNP:754088842
1078 1078 c, t dbSNP:141630472
1081 1081 a, c, t dbSNP:62193614
1082 1082 c, g dbSNP:750002102
1086 1086 a, g dbSNP:766985383
1089 1089 c, t dbSNP:370928521
1099 1099 c, t dbSNP:201477228
1100 1100 a, g dbSNP:188499120
1105 1105 a, g, t dbSNP:762820641
1109 1109 a, g dbSNP:775133036
1115 1115 a, g dbSNP:771247780
1117 1117 a, g dbSNP:567401796
1118 1118 a, g dbSNP:747198657
1123 1123 a, c dbSNP:138458978
1133 1133 c, t dbSNP:772297042
1134 1134 a, g dbSNP:773327968
1145 1145 c, t dbSNP:267599174
1147 1147 a, g dbSNP:779481497
1149 1149 c, g dbSNP:755385980
1152 1152 -, t dbSNP:767852664
1155 1155 c, t dbSNP:754290687
1159 1159 c, t dbSNP:377475055
1164 1164 c, t dbSNP:202210066
1165 1165 a, g dbSNP:199915613
1170 1170 a, g dbSNP:113732076
1181 1181 a, g dbSNP:148160119
1182 1182 c, t dbSNP:185564694
1186 1186 c, t dbSNP:767109945
1196 1196 c, t dbSNP:761315922
1209 1209 g, t dbSNP:763478345
1211 1211 c, t dbSNP:758405875
1220 1220 a, c dbSNP:752595957
1228 1228 a, c dbSNP:765179388
1243 1243 c, t dbSNP:759413310
1250 1250 c, g dbSNP:200718328
1258 1258 a, g dbSNP:776513405
1264 1264 a, c, g dbSNP:762126667
1266 1266 g, t dbSNP:536880332
1273 1273 c, t dbSNP:768596300
1278 1278 -, g dbSNP:766547920
1281 1281 c, t dbSNP:762867621
1282 1282 a, t dbSNP:775893896
1293 1293 g, t dbSNP:770226824
1296 1296 a, g dbSNP:372711897
1300 1300 a, t dbSNP:781533021
1301 1301 a, g dbSNP:756940135
1304 1304 a, g dbSNP:746615557
1305 1305 a, g dbSNP:777431979
1306 1306 c, t dbSNP:200591678
1312 1312 c, t dbSNP:752187592
1321 1321 c, t dbSNP:765282142
1324 1324 a, c dbSNP:754957038
1325 1325 c, t dbSNP:369234651
1326 1326 a, g dbSNP:201267625
1335 1335 g, t dbSNP:775128585
1339 1339 c, t dbSNP:766246232
1347 1347 a, t dbSNP:762143982
1349 1349 -, a dbSNP:763043045
1355 1355 a, c dbSNP:141895800
1357 1357 c, t dbSNP:764338499
1358 1358 c, t dbSNP:762954264
1359 1359 a, g dbSNP:775623666
1361 1361 -, ttacccaat dbSNP:771971686
1361 1361 c, t dbSNP:760963029
1369 1369 a, g dbSNP:566072712
1388 1388 a, g dbSNP:771692756
1401 1401 c, g dbSNP:747809723
1402 1402 a, g dbSNP:778615461
1408 1408 c, t dbSNP:768304856
1449 1449 -, t dbSNP:759303223
1451 1451 a, c dbSNP:3778
1457 1457 c, t dbSNP:748768472
1464 1464 c, g dbSNP:780146398
1465 1465 c, t dbSNP:114939378
1466 1466 a, t dbSNP:750251172
1476 1476 a, g dbSNP:781009655
1497 1497 a, t dbSNP:758597026
1501 1501 c, g dbSNP:200122564
1521 1521 c, t dbSNP:768957576
1530 1530 -, a dbSNP:532947500
1558 1558 g, t dbSNP:185188955
1567 1567 a, g dbSNP:112722672
1587 1587 a, c dbSNP:749424817
1605 1605 c, t dbSNP:78539882
1610 1610 c, t dbSNP:533578934
1671 1671 -, atttt dbSNP:781093485
1745 1745 a, t dbSNP:564140008
1746 1746 c, t dbSNP:369898908
1787 1787 a, g dbSNP:1044416
1845 1845 c, g dbSNP:561722525
1875 1875 c, g dbSNP:746228832
1942 1942 a, t dbSNP:13026
1943 1943 g, t dbSNP:6730955
2033 2033 a, g dbSNP:150906753
2051 2051 c, t dbSNP:575807296
2052 2052 a, g dbSNP:180928167
2063 2063 g, t dbSNP:557950423
2091 2091 c, g dbSNP:16841606
2123 2123 a, t dbSNP:747993945
2127 2127 a, g dbSNP:576632204
2155 2155 c, t dbSNP:557788219
2158 2158 c, t dbSNP:15344
2189 2189 a, c dbSNP:369225330

Target ORF information:

RefSeq Version NM_001282386
Organism Homo sapiens (human)
Definition Homo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282386

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCCAAAA AAATCAGTGG CGGTTCTGTG GTAGAGATGC AAGGAGATGA AATGACACGA 
ATCATTTGGG AATTGATTAA AGAGAAACTC ATTTTTCCCT ACGTGGAATT GGATCTACAT
AGCTATGATT TAGGCATAGA GAATCGTGAT GCCACCAACG ACCAAGTCAC CAAGGATGCT
GCAGAAGCTA TAAAGAAGCA TAATGTTGGC GTCAAATGTG CCACTATCAC TCCTGATGAG
AAGAGGGTTG AGGAGTTCAA GTTGAAACAA ATGTGGAAAT CACCAAATGG CACCATACGA
AATATTCTGG GTGGCACGGT CTTCAGAGAA GCCATTATCT GCAAAAATAT CCCCCGGCTT
GTGAGTGGAT GGGTAAAACC TATCATCATA GGTCGTCATG CTTATGGGGA TCAATACAGA
GCAACTGATT TTGTTGTTCC TGGGCCTGGA AAAGTAGAGA TAACCTACAC ACCAAGTGAC
GGAACCCAAA AGGTGACATA CCTGGTACAT AACTTTGAAG AAGGTGGTGG TGTTGCCATG
GGGATGTATA ATCAAGATAA GTCAATTGAA GATTTTGCAC ACAGTTCCTT CCAAATGGCT
CTGTCTAAGG GTTGGCCTTT GTATCTGAGC ACCAAAAACA CTATTCTGAA GAAATATGAT
GGGCGTTTTA AAGACATCTT TCAGGAGATA TATGACAAGC AGTACAAGTC CCAGTTTGAA
GCTCAAAAGA TCTGGTATGA GCATAGGCTC ATCGACGACA TGGTGGCCCA AGCTATGAAA
TCAGAGGGAG GCTTCATCTG GGCCTGTAAA AACTATGATG GTGACGTGCA GTCGGACTCT
GTGGCCCAAG GGTATGGCTC TCTCGGCATG ATGACCAGCG TGCTGGTTTG TCCAGATGGC
AAGACAGTAG AAGCAGAGGC TGCCCACGGG ACTGTAACCC GTCACTACCG CATGTACCAG
AAAGGACAGG AGACGTCCAC CAATCCCATT GCTTCCATTT TTGCCTGGAC CAGAGGGTTA
GCCCACAGAG CAAAGCTTGA TAACAATAAA GAGCTTGCCT TCTTTGCAAA TGCTTTGGAA
GAAGTCTCTA TTGAGACAAT TGAGGCTGGC TTCATGACCA AGGACTTGGC TGCTTGCATT
AAAGGTTTAC CCAATGTGCA ACGTTCTGAC TACTTGAATA CATTTGAGTT CATGGATAAA
CTTGGAGAAA ACTTGAAGAT CAAACTAGCT CAGGCCAAAC TTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.