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RSPO4 R-spondin 4 [Homo sapiens (human)]

Gene Symbol RSPO4
Entrez Gene ID 343637
Full Name R-spondin 4
Synonyms C20orf182, CRISTIN4
General protein information
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

20

20p13

Summary This gene encodes a member of the R-spondin family of proteins that share a common domain organization consisting of a signal peptide, cysteine-rich/furin-like domain, thrombospondin domain and a C-terminal basic region. The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated with anonychia congenital. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009]. lac of sum
Disorder MIM:

610573

Disorder Html: Anonychia congenita, 206800 (3)





The following RSPO4 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RSPO4 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu79433 XM_011529232 PREDICTED: Homo sapiens R-spondin 4 (RSPO4), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu79434 XM_011529233 PREDICTED: Homo sapiens R-spondin 4 (RSPO4), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99
OHu20587 NM_001029871 Homo sapiens R-spondin 4 (RSPO4), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu00129 NM_001040007 Homo sapiens R-spondin 4 (RSPO4), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu79433
Accession Version XM_011529232.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 753bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product R-spondin-4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011387.9) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGCGGGCGC CACTCTGCCT GCTCCTGCTC GTCGCCCACG CCGTGGACAT GCTCGCCCTG 
AACCGAAGGA AGAAGCAAGG GCAGCCTCTG TGCCAGGCTC TGTGTTGGGG ACCTTGGAGA
TACCCAGTGG GCACTGGCCT GGGGGGCAAC TGCACAGGCT GTATCATCTG CTCAGAGGAG
AACGGCTGTT CCACCTGCCA GCAGAGGCTC TTCCTGTTCA TCCGCCGGGA AGGCATCCGC
CAGTACGGCA AGTGCCTGCA CGACTGTCCC CCTGGGTACT TCGGCATCCG CGGCCAGGAG
GTCAACAGGT GCAAAAAATG TGGGGCCACT TGTGAGAGCT GCTTCAGCCA GGACTTCTGC
ATCCGGTGCA AGAGGCAGTT TTACTTGTAC AAGGGGAAGT GTCTGCCCAC CTGCCCGCCG
GGCACTTTGG CCCACCAGAA CACACGGGAG TGCCAGGGGG AGTGTGAACT GGGTCCCTGG
GGCGGCTGGA GCCCCTGCAC ACACAATGGA AAGACCTGCG GCTCGGCTTG GGGCCTGGAG
AGCCGGGTAC GAGAGGCTGG CCGGGCTGGG CATGAGGAGG CAGCCACCTG CCAGGTGCTT
TCTGAGTCAA GGAAATGTCC CATCCAGAGG CCCTGCCCAG GAGAGAGGAG CCCCGGCCAG
AAGAAGGGCA GGAAGGACCG GCGCCCACGC AAGGACAGGA AGCTGGACCG CAGGCTGGAC
GTGAGGCCGC GCCAGCCCGG CCTGCAGCCC TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011527534.1
CDS121..873
Misc Feature(1)436..576(+)
Translation
Position Chain Variation Link
6 6 a, g dbSNP:149154047
25 25 a, g dbSNP:552497905
33 33 c, g dbSNP:112044127
36 36 a, c, t dbSNP:6056520
82 82 a, g dbSNP:560184549
85 85 c, t dbSNP:768594197
90 90 c, g dbSNP:749426265
104 104 c, g dbSNP:780311755
110 110 c, t dbSNP:756348305
121 121 a, t dbSNP:746093558
127 127 a, g dbSNP:781688494
132 132 a, c dbSNP:150446609
150 150 c, t dbSNP:751990801
154 154 a, g dbSNP:373126184
160 160 a, g, t dbSNP:200584918
162 162 c, t dbSNP:6086831
163 163 a, g dbSNP:752157295
168 168 c, t dbSNP:530215228
171 171 g, t dbSNP:759118523
178 178 c, g dbSNP:776329354
183 183 a, c dbSNP:766071797
189 189 a, g dbSNP:760566194
193 193 a, c dbSNP:772989424
195 195 a, g dbSNP:772005883
196 196 c, g dbSNP:761856917
243 243 c, t dbSNP:751960093
254 254 c, g dbSNP:761838651
261 261 g, t dbSNP:774489748
262 262 a, g dbSNP:199528639
266 266 -, g dbSNP:768138495
275 275 c, g, t dbSNP:776608135
279 279 a, c dbSNP:771139553
281 281 a, g dbSNP:377060056
282 282 c, t dbSNP:747212538
283 283 a, g dbSNP:763333338
289 289 c, t dbSNP:778130157
294 294 a, g dbSNP:550978379
295 295 a, g dbSNP:748638528
297 297 a, g dbSNP:201142150
298 298 a, g dbSNP:753113241
302 302 a, g dbSNP:753303855
304 304 a, g dbSNP:375035609
309 309 g, t dbSNP:755726509
313 313 a, c dbSNP:201933377
321 321 a, g dbSNP:767332107
323 323 a, g dbSNP:761750744
327 327 c, g, t dbSNP:371949198
329 329 a, t dbSNP:764084804
330 330 c, g dbSNP:763011320
333 333 c, g dbSNP:776717734
334 334 c, t dbSNP:766370306
336 336 a, g dbSNP:760943314
339 339 c, t dbSNP:773361802
340 340 a, g dbSNP:200792773
342 342 c, t dbSNP:748550698
343 343 c, t dbSNP:199844911
346 346 c, t dbSNP:769102112
352 352 a, g dbSNP:372674964
358 358 c, t dbSNP:387907027
359 359 a, g dbSNP:755637414
360 360 a, c dbSNP:745514631
362 362 a, g dbSNP:74315420
364 364 a, t dbSNP:757004626
366 366 c, t dbSNP:751352700
367 367 c, g dbSNP:387907028
368 368 g, t dbSNP:763998727
370 370 a, g dbSNP:758348841
371 371 a, t dbSNP:752640647
374 374 g, t dbSNP:766465255
375 375 c, t dbSNP:760715487
379 379 a, c dbSNP:773450068
382 382 a, c, g dbSNP:368559955
384 384 c, t dbSNP:762043573
386 386 a, g dbSNP:74315423
388 388 a, c, t dbSNP:769012270
390 390 c, t dbSNP:749789537
391 391 a, c dbSNP:775912164
402 402 c, t dbSNP:148789929
403 403 a, g dbSNP:542994015
410 410 a, g dbSNP:780874153
411 411 c, t dbSNP:756916549
412 412 a, c, g, t dbSNP:201062485
417 417 a, g dbSNP:574261920
426 426 c, t dbSNP:752655381
429 429 a, g dbSNP:765124151
433 433 a, g dbSNP:370469988
440 440 a, g dbSNP:765642242
441 441 -, tgg dbSNP:777661973
452 452 g, t dbSNP:780506366
453 453 c, t dbSNP:777330925
456 456 c, g dbSNP:770539145
463 463 g, t dbSNP:763385829
469 469 c, t dbSNP:387907026
470 470 a, g dbSNP:760299043
472 472 a, g dbSNP:772877858
473 473 a, c, g dbSNP:529579675
483 483 c, t dbSNP:778504126
484 484 c, t dbSNP:202132004
485 485 a, g dbSNP:6140807
487 487 c, t dbSNP:74315421
494 494 c, g dbSNP:755441249
495 495 a, g dbSNP:751592558
502 502 c, t dbSNP:377288758
506 506 c, t dbSNP:574845619
507 507 a, g dbSNP:753033719
510 510 c, t dbSNP:765511134
516 516 a, g dbSNP:755439339
521 521 a, g dbSNP:74315422
522 522 c, t dbSNP:564534973
527 527 c, t dbSNP:200597556
528 528 c, t dbSNP:199861662
535 535 c, g dbSNP:201485021
536 536 c, t dbSNP:370909673
537 537 a, g dbSNP:184279006
539 539 c, t dbSNP:774111255
540 540 a, g dbSNP:77472961
541 541 a, g dbSNP:369045323
542 542 a, g dbSNP:573862295
545 545 c, t dbSNP:201112120
546 546 g, t dbSNP:745812419
552 552 c, t dbSNP:777713947
564 564 a, t dbSNP:758567274
565 565 c, t dbSNP:200568022
566 566 a, g dbSNP:779231306
567 567 a, g dbSNP:571711499
577 577 a, g dbSNP:373277668
592 592 a, g dbSNP:531525130
602 602 g, t dbSNP:568780057
603 603 c, t dbSNP:201319898
604 604 a, g dbSNP:377299234
615 615 c, t dbSNP:764990656
618 618 a, c dbSNP:759420136
622 622 c, t dbSNP:576148793
623 623 a, c dbSNP:191521473
629 629 a, g, t dbSNP:560532338
636 636 c, t dbSNP:774425406
639 639 c, t dbSNP:41275604
640 640 a, g dbSNP:749509229
645 645 a, g dbSNP:780473273
650 650 a, g dbSNP:373745354
653 653 a, g dbSNP:746193611
660 660 a, g dbSNP:781423079
664 664 c, t dbSNP:757714366
665 665 a, g dbSNP:201621545
666 666 a, g dbSNP:777248092
667 667 -, g dbSNP:766345914
670 670 c, t dbSNP:757948155
671 671 a, g dbSNP:752310739
673 673 a, g dbSNP:147416132
676 676 a, g dbSNP:759136246
681 681 c, t dbSNP:753613363
682 682 c, t dbSNP:368089169
683 683 a, c, g dbSNP:561493674
684 684 a, g dbSNP:768835667
692 692 a, c, t dbSNP:61740632
694 694 a, g dbSNP:769999094
696 696 a, g dbSNP:199601701
712 712 c, t dbSNP:777047218
713 713 a, g dbSNP:559253642
714 714 g, t dbSNP:747525884
715 715 c, g dbSNP:778288742
722 722 c, t dbSNP:758938963
728 728 c, g dbSNP:747612348
732 732 a, g dbSNP:370873974
738 738 g, t dbSNP:376404996
739 739 c, t dbSNP:138935370
746 746 a, g dbSNP:371086524
752 752 c, t dbSNP:748229508
757 757 c, t dbSNP:553713151
775 775 a, g dbSNP:755822660
788 788 a, g dbSNP:750184862
799 799 c, t dbSNP:767423520
800 800 a, g dbSNP:757255412
802 802 c, t dbSNP:202178299
803 803 a, g dbSNP:765335066
806 806 c, t dbSNP:374130199
808 808 c, t dbSNP:369687640
809 809 a, g dbSNP:377200892
819 819 a, g dbSNP:760903291
820 820 a, c dbSNP:773501751
829 829 c, t dbSNP:772494608
830 830 a, g dbSNP:762295741
834 834 g, t dbSNP:774771039
840 840 c, t dbSNP:759063553
841 841 c, g dbSNP:768182696
848 848 a, c, t dbSNP:201162335
849 849 a, g dbSNP:369860447
850 850 c, t dbSNP:745580942
851 851 a, g dbSNP:781119266
857 857 c, t dbSNP:375728608
858 858 c, t dbSNP:751507935
859 859 a, g dbSNP:563843176
875 875 c, t dbSNP:113093200
883 883 c, g dbSNP:534275132
886 886 -, ccgactctc dbSNP:573730556
887 887 a, c dbSNP:766487997
906 906 c, t dbSNP:747967944
909 909 a, g dbSNP:565675207
910 910 a, g dbSNP:767846748
915 915 c, t dbSNP:6118370
930 930 a, g dbSNP:549138091
1016 1016 a, g dbSNP:376648133
1022 1022 a, c dbSNP:563500394
1030 1030 c, g dbSNP:577512032

Target ORF information:

RefSeq Version XM_011529232
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens R-spondin 4 (RSPO4), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529232

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGCGGGCGC CACTCTGCCT GCTCCTGCTC GTCGCCCACG CCGTGGACAT GCTCGCCCTG 
AACCGAAGGA AGAAGCAAGG GCAGCCTCTG TGCCAGGCTC TGTGTTGGGG ACCTTGGAGA
TACCCAGTGG GCACTGGCCT GGGGGGCAAC TGCACAGGCT GTATCATCTG CTCAGAGGAG
AACGGCTGTT CCACCTGCCA GCAGAGGCTC TTCCTGTTCA TCCGCCGGGA AGGCATCCGC
CAGTACGGCA AGTGCCTGCA CGACTGTCCC CCTGGGTACT TCGGCATCCG CGGCCAGGAG
GTCAACAGGT GCAAAAAATG TGGGGCCACT TGTGAGAGCT GCTTCAGCCA GGACTTCTGC
ATCCGGTGCA AGAGGCAGTT TTACTTGTAC AAGGGGAAGT GTCTGCCCAC CTGCCCGCCG
GGCACTTTGG CCCACCAGAA CACACGGGAG TGCCAGGGGG AGTGTGAACT GGGTCCCTGG
GGCGGCTGGA GCCCCTGCAC ACACAATGGA AAGACCTGCG GCTCGGCTTG GGGCCTGGAG
AGCCGGGTAC GAGAGGCTGG CCGGGCTGGG CATGAGGAGG CAGCCACCTG CCAGGTGCTT
TCTGAGTCAA GGAAATGTCC CATCCAGAGG CCCTGCCCAG GAGAGAGGAG CCCCGGCCAG
AAGAAGGGCA GGAAGGACCG GCGCCCACGC AAGGACAGGA AGCTGGACCG CAGGCTGGAC
GTGAGGCCGC GCCAGCCCGG CCTGCAGCCC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu79434
Accession Version XM_011529233.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 567bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product R-spondin-4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011387.9) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
ATGCGGGCGC CACTCTGCCT GCTCCTGCTC GTCGCCCACG CCGTGGACAT GCTCGCCCTG 
AACCGAAGGA AGAAGCAAGG GCAGCCTCTG TGCCAGGCTC TGTGTTGGGG ACCTTGGAGA
TACCCAGTGG GCACTGGCCT GGGGGGCAAC TGCACAGGCT GTATCATCTG CTCAGAGGAG
AACGGCTGTT CCACCTGCCA GCAGAGGCTC TTCCTGTTCA TCCGCCGGGA AGGCATCCGC
CAGTACGGCA AGTGCCTGCA CGACTGTCCC CCTGGGTACT TCGGCATCCG CGGCCAGGAG
GTCAACAGGT GCAAAAAATG TGGGGCCACT TGTGAGAGCT GCTTCAGCCA GGACTTCTGC
ATCCGGTGCA AGAGGCAGTT TTACTTGTAC AAGGGGAAGT GTCTGCCCAC CTGCCCGCCG
GGCACTTTGG CCCACCAGAA CACACGGGAG TGCCAGGAGA GGAGCCCCGG CCAGAAGAAG
GGCAGGAAGG ACCGGCGCCC ACGCAAGGAC AGGAAGCTGG ACCGCAGGCT GGACGTGAGG
CCGCGCCAGC CCGGCCTGCA GCCCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011527535.1
CDS121..687
Misc Feature(1)436..576(+)
Translation
Position Chain Variation Link
6 6 a, g dbSNP:149154047
25 25 a, g dbSNP:552497905
33 33 c, g dbSNP:112044127
36 36 a, c, t dbSNP:6056520
82 82 a, g dbSNP:560184549
85 85 c, t dbSNP:768594197
90 90 c, g dbSNP:749426265
104 104 c, g dbSNP:780311755
110 110 c, t dbSNP:756348305
121 121 a, t dbSNP:746093558
127 127 a, g dbSNP:781688494
132 132 a, c dbSNP:150446609
150 150 c, t dbSNP:751990801
154 154 a, g dbSNP:373126184
160 160 a, g, t dbSNP:200584918
162 162 c, t dbSNP:6086831
163 163 a, g dbSNP:752157295
168 168 c, t dbSNP:530215228
171 171 g, t dbSNP:759118523
178 178 c, g dbSNP:776329354
183 183 a, c dbSNP:766071797
189 189 a, g dbSNP:760566194
193 193 a, c dbSNP:772989424
195 195 a, g dbSNP:772005883
196 196 c, g dbSNP:761856917
243 243 c, t dbSNP:751960093
254 254 c, g dbSNP:761838651
261 261 g, t dbSNP:774489748
262 262 a, g dbSNP:199528639
266 266 -, g dbSNP:768138495
275 275 c, g, t dbSNP:776608135
279 279 a, c dbSNP:771139553
281 281 a, g dbSNP:377060056
282 282 c, t dbSNP:747212538
283 283 a, g dbSNP:763333338
289 289 c, t dbSNP:778130157
294 294 a, g dbSNP:550978379
295 295 a, g dbSNP:748638528
297 297 a, g dbSNP:201142150
298 298 a, g dbSNP:753113241
302 302 a, g dbSNP:753303855
304 304 a, g dbSNP:375035609
309 309 g, t dbSNP:755726509
313 313 a, c dbSNP:201933377
321 321 a, g dbSNP:767332107
323 323 a, g dbSNP:761750744
327 327 c, g, t dbSNP:371949198
329 329 a, t dbSNP:764084804
330 330 c, g dbSNP:763011320
333 333 c, g dbSNP:776717734
334 334 c, t dbSNP:766370306
336 336 a, g dbSNP:760943314
339 339 c, t dbSNP:773361802
340 340 a, g dbSNP:200792773
342 342 c, t dbSNP:748550698
343 343 c, t dbSNP:199844911
346 346 c, t dbSNP:769102112
352 352 a, g dbSNP:372674964
358 358 c, t dbSNP:387907027
359 359 a, g dbSNP:755637414
360 360 a, c dbSNP:745514631
362 362 a, g dbSNP:74315420
364 364 a, t dbSNP:757004626
366 366 c, t dbSNP:751352700
367 367 c, g dbSNP:387907028
368 368 g, t dbSNP:763998727
370 370 a, g dbSNP:758348841
371 371 a, t dbSNP:752640647
374 374 g, t dbSNP:766465255
375 375 c, t dbSNP:760715487
379 379 a, c dbSNP:773450068
382 382 a, c, g dbSNP:368559955
384 384 c, t dbSNP:762043573
386 386 a, g dbSNP:74315423
388 388 a, c, t dbSNP:769012270
390 390 c, t dbSNP:749789537
391 391 a, c dbSNP:775912164
402 402 c, t dbSNP:148789929
403 403 a, g dbSNP:542994015
410 410 a, g dbSNP:780874153
411 411 c, t dbSNP:756916549
412 412 a, c, g, t dbSNP:201062485
417 417 a, g dbSNP:574261920
426 426 c, t dbSNP:752655381
429 429 a, g dbSNP:765124151
433 433 a, g dbSNP:370469988
440 440 a, g dbSNP:765642242
441 441 -, tgg dbSNP:777661973
452 452 g, t dbSNP:780506366
453 453 c, t dbSNP:777330925
456 456 c, g dbSNP:770539145
463 463 g, t dbSNP:763385829
469 469 c, t dbSNP:387907026
470 470 a, g dbSNP:760299043
472 472 a, g dbSNP:772877858
473 473 a, c, g dbSNP:529579675
483 483 c, t dbSNP:778504126
484 484 c, t dbSNP:202132004
485 485 a, g dbSNP:6140807
487 487 c, t dbSNP:74315421
494 494 c, g dbSNP:755441249
495 495 a, g dbSNP:751592558
502 502 c, t dbSNP:377288758
506 506 c, t dbSNP:574845619
507 507 a, g dbSNP:753033719
510 510 c, t dbSNP:765511134
516 516 a, g dbSNP:755439339
521 521 a, g dbSNP:74315422
522 522 c, t dbSNP:564534973
527 527 c, t dbSNP:200597556
528 528 c, t dbSNP:199861662
535 535 c, g dbSNP:201485021
536 536 c, t dbSNP:370909673
537 537 a, g dbSNP:184279006
539 539 c, t dbSNP:774111255
540 540 a, g dbSNP:77472961
541 541 a, g dbSNP:369045323
542 542 a, g dbSNP:573862295
545 545 c, t dbSNP:201112120
546 546 g, t dbSNP:745812419
552 552 c, t dbSNP:777713947
564 564 a, t dbSNP:758567274
565 565 c, t dbSNP:200568022
566 566 a, g dbSNP:779231306
567 567 a, g dbSNP:571711499
577 577 a, g dbSNP:373277668
589 589 a, g dbSNP:755822660
602 602 a, g dbSNP:750184862
613 613 c, t dbSNP:767423520
614 614 a, g dbSNP:757255412
616 616 c, t dbSNP:202178299
617 617 a, g dbSNP:765335066
620 620 c, t dbSNP:374130199
622 622 c, t dbSNP:369687640
623 623 a, g dbSNP:377200892
633 633 a, g dbSNP:760903291
634 634 a, c dbSNP:773501751
643 643 c, t dbSNP:772494608
644 644 a, g dbSNP:762295741
648 648 g, t dbSNP:774771039
654 654 c, t dbSNP:759063553
655 655 c, g dbSNP:768182696
662 662 a, c, t dbSNP:201162335
663 663 a, g dbSNP:369860447
664 664 c, t dbSNP:745580942
665 665 a, g dbSNP:781119266
671 671 c, t dbSNP:375728608
672 672 c, t dbSNP:751507935
673 673 a, g dbSNP:563843176
689 689 c, t dbSNP:113093200
697 697 c, g dbSNP:534275132
700 700 -, ccgactctc dbSNP:573730556
701 701 a, c dbSNP:766487997
720 720 c, t dbSNP:747967944
723 723 a, g dbSNP:565675207
724 724 a, g dbSNP:767846748
729 729 c, t dbSNP:6118370
744 744 a, g dbSNP:549138091
830 830 a, g dbSNP:376648133
836 836 a, c dbSNP:563500394
844 844 c, g dbSNP:577512032

Target ORF information:

RefSeq Version XM_011529233
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens R-spondin 4 (RSPO4), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529233

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGCGGGCGC CACTCTGCCT GCTCCTGCTC GTCGCCCACG CCGTGGACAT GCTCGCCCTG 
AACCGAAGGA AGAAGCAAGG GCAGCCTCTG TGCCAGGCTC TGTGTTGGGG ACCTTGGAGA
TACCCAGTGG GCACTGGCCT GGGGGGCAAC TGCACAGGCT GTATCATCTG CTCAGAGGAG
AACGGCTGTT CCACCTGCCA GCAGAGGCTC TTCCTGTTCA TCCGCCGGGA AGGCATCCGC
CAGTACGGCA AGTGCCTGCA CGACTGTCCC CCTGGGTACT TCGGCATCCG CGGCCAGGAG
GTCAACAGGT GCAAAAAATG TGGGGCCACT TGTGAGAGCT GCTTCAGCCA GGACTTCTGC
ATCCGGTGCA AGAGGCAGTT TTACTTGTAC AAGGGGAAGT GTCTGCCCAC CTGCCCGCCG
GGCACTTTGG CCCACCAGAA CACACGGGAG TGCCAGGAGA GGAGCCCCGG CCAGAAGAAG
GGCAGGAAGG ACCGGCGCCC ACGCAAGGAC AGGAAGCTGG ACCGCAGGCT GGACGTGAGG
CCGCGCCAGC CCGGCCTGCA GCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20587
Accession Version NM_001029871.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 705bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product R-spondin-4 isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL050325.20, AK122609.1 and BQ185331.1. This sequence is a reference standard in the RefSeqGene project. On Sep 29, 2009 this sequence version replaced gi:83722283. Summary: This gene encodes a member of the R-spondin family of proteins that share a common domain organization consisting of a signal peptide, cysteine-rich/furin-like domain, thrombospondin domain and a C-terminal basic region. The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated with anonychia congenital. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK122609.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142363, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGCGGGCGC CACTCTGCCT GCTCCTGCTC GTCGCCCACG CCGTGGACAT GCTCGCCCTG 
AACCGAAGGA AGAAGCAAGT GGGCACTGGC CTGGGGGGCA ACTGCACAGG CTGTATCATC
TGCTCAGAGG AGAACGGCTG TTCCACCTGC CAGCAGAGGC TCTTCCTGTT CATCCGCCGG
GAAGGCATCC GCCAGTACGG CAAGTGCCTG CACGACTGTC CCCCTGGGTA CTTCGGCATC
CGCGGCCAGG AGGTCAACAG GTGCAAAAAA TGTGGGGCCA CTTGTGAGAG CTGCTTCAGC
CAGGACTTCT GCATCCGGTG CAAGAGGCAG TTTTACTTGT ACAAGGGGAA GTGTCTGCCC
ACCTGCCCGC CGGGCACTTT GGCCCACCAG AACACACGGG AGTGCCAGGG GGAGTGTGAA
CTGGGTCCCT GGGGCGGCTG GAGCCCCTGC ACACACAATG GAAAGACCTG CGGCTCGGCT
TGGGGCCTGG AGAGCCGGGT ACGAGAGGCT GGCCGGGCTG GGCATGAGGA GGCAGCCACC
TGCCAGGTGC TTTCTGAGTC AAGGAAATGT CCCATCCAGA GGCCCTGCCC AGGAGAGAGG
AGCCCCGGCC AGAAGAAGGG CAGGAAGGAC CGGCGCCCAC GCAAGGACAG GAAGCTGGAC
CGCAGGCTGG ACGTGAGGCC GCGCCAGCCC GGCCTGCAGC CCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001025042.2
CDS101..805
Misc Feature(1)353..484(+)
Misc Feature(2)368..508(+)
Exon (1)1..179
Gene:RSPO4
Gene Synonym:
Exon (2)180..368
Gene:RSPO4
Gene Synonym:
Exon (3)369..509
Gene:RSPO4
Gene Synonym:
Exon (4)510..695
Gene:RSPO4
Gene Synonym:
Exon (5)696..2710
Gene:RSPO4
Gene Synonym:
Translation
Position Chain Variation Link
5 5 a, g dbSNP:552497905
13 13 c, g dbSNP:112044127
16 16 a, c, t dbSNP:6056520
62 62 a, g dbSNP:560184549
65 65 c, t dbSNP:768594197
70 70 c, g dbSNP:749426265
84 84 c, g dbSNP:780311755
90 90 c, t dbSNP:756348305
101 101 a, t dbSNP:746093558
107 107 a, g dbSNP:781688494
112 112 a, c dbSNP:150446609
130 130 c, t dbSNP:751990801
134 134 a, g dbSNP:373126184
140 140 a, g, t dbSNP:200584918
142 142 c, t dbSNP:6086831
143 143 a, g dbSNP:752157295
148 148 c, t dbSNP:530215228
151 151 g, t dbSNP:759118523
158 158 c, g dbSNP:776329354
163 163 a, c dbSNP:766071797
169 169 a, g dbSNP:760566194
173 173 a, c dbSNP:772989424
175 175 a, g dbSNP:772005883
176 176 c, g dbSNP:761856917
186 186 c, g dbSNP:761838651
193 193 g, t dbSNP:774489748
194 194 a, g dbSNP:199528639
198 198 -, g dbSNP:768138495
207 207 c, g, t dbSNP:776608135
211 211 a, c dbSNP:771139553
213 213 a, g dbSNP:377060056
214 214 c, t dbSNP:747212538
215 215 a, g dbSNP:763333338
221 221 c, t dbSNP:778130157
226 226 a, g dbSNP:550978379
227 227 a, g dbSNP:748638528
229 229 a, g dbSNP:201142150
230 230 a, g dbSNP:753113241
234 234 a, g dbSNP:753303855
236 236 a, g dbSNP:375035609
241 241 g, t dbSNP:755726509
245 245 a, c dbSNP:201933377
253 253 a, g dbSNP:767332107
255 255 a, g dbSNP:761750744
259 259 c, g, t dbSNP:371949198
261 261 a, t dbSNP:764084804
262 262 c, g dbSNP:763011320
265 265 c, g dbSNP:776717734
266 266 c, t dbSNP:766370306
268 268 a, g dbSNP:760943314
271 271 c, t dbSNP:773361802
272 272 a, g dbSNP:200792773
274 274 c, t dbSNP:748550698
275 275 c, t dbSNP:199844911
278 278 c, t dbSNP:769102112
284 284 a, g dbSNP:372674964
290 290 c, t dbSNP:387907027
291 291 a, g dbSNP:755637414
292 292 a, c dbSNP:745514631
294 294 a, g dbSNP:74315420
296 296 a, t dbSNP:757004626
298 298 c, t dbSNP:751352700
299 299 c, g dbSNP:387907028
300 300 g, t dbSNP:763998727
302 302 a, g dbSNP:758348841
303 303 a, t dbSNP:752640647
306 306 g, t dbSNP:766465255
307 307 c, t dbSNP:760715487
311 311 a, c dbSNP:773450068
314 314 a, c, g dbSNP:368559955
316 316 c, t dbSNP:762043573
318 318 a, g dbSNP:74315423
320 320 a, c, t dbSNP:769012270
322 322 c, t dbSNP:749789537
323 323 a, c dbSNP:775912164
334 334 c, t dbSNP:148789929
335 335 a, g dbSNP:542994015
342 342 a, g dbSNP:780874153
343 343 c, t dbSNP:756916549
344 344 a, c, g, t dbSNP:201062485
349 349 a, g dbSNP:574261920
358 358 c, t dbSNP:752655381
361 361 a, g dbSNP:765124151
365 365 a, g dbSNP:370469988
372 372 a, g dbSNP:765642242
373 373 -, tgg dbSNP:777661973
384 384 g, t dbSNP:780506366
385 385 c, t dbSNP:777330925
388 388 c, g dbSNP:770539145
395 395 g, t dbSNP:763385829
401 401 c, t dbSNP:387907026
402 402 a, g dbSNP:760299043
404 404 a, g dbSNP:772877858
405 405 a, c, g dbSNP:529579675
415 415 c, t dbSNP:778504126
416 416 c, t dbSNP:202132004
417 417 a, g dbSNP:6140807
419 419 c, t dbSNP:74315421
426 426 c, g dbSNP:755441249
427 427 a, g dbSNP:751592558
434 434 c, t dbSNP:377288758
438 438 c, t dbSNP:574845619
439 439 a, g dbSNP:753033719
442 442 c, t dbSNP:765511134
448 448 a, g dbSNP:755439339
453 453 a, g dbSNP:74315422
454 454 c, t dbSNP:564534973
459 459 c, t dbSNP:200597556
460 460 c, t dbSNP:199861662
467 467 c, g dbSNP:201485021
468 468 c, t dbSNP:370909673
469 469 a, g dbSNP:184279006
471 471 c, t dbSNP:774111255
472 472 a, g dbSNP:77472961
473 473 a, g dbSNP:369045323
474 474 a, g dbSNP:573862295
477 477 c, t dbSNP:201112120
478 478 g, t dbSNP:745812419
484 484 c, t dbSNP:777713947
496 496 a, t dbSNP:758567274
497 497 c, t dbSNP:200568022
498 498 a, g dbSNP:779231306
499 499 a, g dbSNP:571711499
509 509 a, g dbSNP:373277668
524 524 a, g dbSNP:531525130
534 534 g, t dbSNP:568780057
535 535 c, t dbSNP:201319898
536 536 a, g dbSNP:377299234
547 547 c, t dbSNP:764990656
550 550 a, c dbSNP:759420136
554 554 c, t dbSNP:576148793
555 555 a, c dbSNP:191521473
561 561 a, g, t dbSNP:560532338
568 568 c, t dbSNP:774425406
571 571 c, t dbSNP:41275604
572 572 a, g dbSNP:749509229
577 577 a, g dbSNP:780473273
582 582 a, g dbSNP:373745354
585 585 a, g dbSNP:746193611
592 592 a, g dbSNP:781423079
596 596 c, t dbSNP:757714366
597 597 a, g dbSNP:201621545
598 598 a, g dbSNP:777248092
599 599 -, g dbSNP:766345914
602 602 c, t dbSNP:757948155
603 603 a, g dbSNP:752310739
605 605 a, g dbSNP:147416132
608 608 a, g dbSNP:759136246
613 613 c, t dbSNP:753613363
614 614 c, t dbSNP:368089169
615 615 a, c, g dbSNP:561493674
616 616 a, g dbSNP:768835667
624 624 a, c, t dbSNP:61740632
626 626 a, g dbSNP:769999094
628 628 a, g dbSNP:199601701
644 644 c, t dbSNP:777047218
645 645 a, g dbSNP:559253642
646 646 g, t dbSNP:747525884
647 647 c, g dbSNP:778288742
654 654 c, t dbSNP:758938963
660 660 c, g dbSNP:747612348
664 664 a, g dbSNP:370873974
670 670 g, t dbSNP:376404996
671 671 c, t dbSNP:138935370
678 678 a, g dbSNP:371086524
684 684 c, t dbSNP:748229508
689 689 c, t dbSNP:553713151
707 707 a, g dbSNP:755822660
720 720 a, g dbSNP:750184862
731 731 c, t dbSNP:767423520
732 732 a, g dbSNP:757255412
734 734 c, t dbSNP:202178299
735 735 a, g dbSNP:765335066
738 738 c, t dbSNP:374130199
740 740 c, t dbSNP:369687640
741 741 a, g dbSNP:377200892
751 751 a, g dbSNP:760903291
752 752 a, c dbSNP:773501751
761 761 c, t dbSNP:772494608
762 762 a, g dbSNP:762295741
766 766 g, t dbSNP:774771039
772 772 c, t dbSNP:759063553
773 773 c, g dbSNP:768182696
780 780 a, c, t dbSNP:201162335
781 781 a, g dbSNP:369860447
782 782 c, t dbSNP:745580942
783 783 a, g dbSNP:781119266
789 789 c, t dbSNP:375728608
790 790 c, t dbSNP:751507935
791 791 a, g dbSNP:563843176
807 807 c, t dbSNP:113093200
815 815 c, g dbSNP:534275132
818 818 -, ccgactctc dbSNP:573730556
819 819 a, c dbSNP:766487997
838 838 c, t dbSNP:747967944
841 841 a, g dbSNP:565675207
842 842 a, g dbSNP:767846748
847 847 c, t dbSNP:6118370
862 862 a, g dbSNP:549138091
948 948 a, g dbSNP:376648133
954 954 a, c dbSNP:563500394
962 962 c, g dbSNP:577512032
991 991 g, t dbSNP:549684296
992 992 c, t dbSNP:34773751
1031 1031 -, ttcc dbSNP:771985596
1109 1109 c, t dbSNP:564186113
1139 1139 c, t dbSNP:76811155
1168 1168 a, g dbSNP:184811687
1170 1170 a, g dbSNP:561739169
1174 1174 c, t dbSNP:192551017
1222 1222 a, g dbSNP:576106571
1248 1248 c, t dbSNP:534453835
1291 1291 a, g dbSNP:519478
1292 1292 c, t dbSNP:539605581
1298 1298 a, c dbSNP:140578124
1301 1301 a, g dbSNP:554214124
1302 1302 a, g dbSNP:776663727
1309 1309 c, g dbSNP:755620946
1341 1341 c, t dbSNP:534495204
1345 1345 a, g dbSNP:745363996
1365 1365 c, t dbSNP:565712072
1367 1367 a, g dbSNP:543936481
1369 1369 g, t dbSNP:778168615
1389 1389 g, t dbSNP:147214985
1405 1405 c, t dbSNP:569735482
1422 1422 c, t dbSNP:549960964
1442 1442 a, g dbSNP:369505776
1443 1443 c, t dbSNP:375151240
1448 1448 a, g dbSNP:547417155
1490 1490 a, g dbSNP:372460184
1493 1493 a, g dbSNP:527566729
1562 1562 c, g dbSNP:561775766
1568 1568 c, t dbSNP:149543960
1573 1573 c, t dbSNP:187647199
1590 1590 c, g dbSNP:562793850
1593 1593 c, t dbSNP:13037150
1599 1599 c, g dbSNP:516714
1610 1610 a, c dbSNP:755386404
1623 1623 c, t dbSNP:554325646
1628 1628 c, g dbSNP:138721103
1633 1633 g, t dbSNP:754044098
1647 1647 a, g dbSNP:575155829
1687 1687 a, g dbSNP:182220957
1694 1694 a, g dbSNP:62187523
1698 1698 a, c dbSNP:552629982
1750 1750 g, t dbSNP:750474691
1779 1779 a, g dbSNP:767445596
1798 1798 a, g dbSNP:556033593
1817 1817 a, g dbSNP:539511973
1845 1845 a, g dbSNP:570590295
1847 1847 c, t dbSNP:509384
1871 1871 c, t dbSNP:527602996
1878 1878 a, g dbSNP:567972603
1907 1907 c, t dbSNP:150625916
1928 1928 a, g dbSNP:190629434
1941 1941 a, c dbSNP:79127824
1949 1949 c, t dbSNP:530194832
1957 1957 c, t dbSNP:529913520
1969 1969 -, tc dbSNP:200388392
1983 1983 a, c dbSNP:76535756
1987 1987 c, t dbSNP:560829312
1991 1991 c, t dbSNP:6108208
2003 2003 c, t dbSNP:62187522
2008 2008 a, g dbSNP:62187521
2027 2027 a, c dbSNP:62187520
2035 2035 c, g dbSNP:6118367
2036 2036 c, g dbSNP:532614806
2049 2049 a, c dbSNP:62187519
2052 2052 a, g dbSNP:560539826
2069 2069 c, t dbSNP:62187518
2074 2074 a, g dbSNP:62187517
2080 2080 c, g dbSNP:58158356
2102 2102 g, t dbSNP:34935852
2133 2133 c, t dbSNP:188433140
2140 2140 a, g dbSNP:13043975
2205 2205 a, g dbSNP:944117
2209 2209 a, g dbSNP:734940
2232 2232 c, g dbSNP:3746813
2265 2265 c, t dbSNP:182992578
2282 2282 a, g dbSNP:944116
2302 2302 c, t dbSNP:553566865
2308 2308 a, g dbSNP:371322363
2324 2324 a, c dbSNP:115673250
2349 2349 c, t dbSNP:555226164
2350 2350 a, g dbSNP:567909164
2370 2370 g, t dbSNP:548118025
2394 2394 c, t dbSNP:531484592
2429 2429 a, g dbSNP:191999547
2479 2479 c, t dbSNP:552513871
2511 2511 c, g dbSNP:3746812
2512 2512 a, c, t dbSNP:574832275
2513 2513 -, c, ct dbSNP:58319408
2513 2513 a, c dbSNP:3746811
2514 2514 -, tc dbSNP:3833342
2514 2514 -, tc dbSNP:397840834
2517 2517 -, c dbSNP:200839031
2544 2544 c, t dbSNP:80086411
2560 2560 c, t dbSNP:377701364
2575 2575 a, c dbSNP:575873978
2586 2586 a, g dbSNP:779528009
2588 2588 a, c dbSNP:562367020
2619 2619 c, g, t dbSNP:569229471
2631 2631 c, t dbSNP:576867453
2639 2639 c, t dbSNP:486347
2641 2641 c, t dbSNP:752576473
2642 2642 a, g dbSNP:373290716
2644 2644 c, t dbSNP:486344
2657 2657 a, g dbSNP:554318960
2660 2660 c, t dbSNP:756291872
2674 2674 a, t dbSNP:554753373
2674 2674 -, t dbSNP:780482661
2675 2675 a, t dbSNP:480012
2678 2678 -, a dbSNP:369731512
2678 2678 a, t dbSNP:552295984
2699 2699 g, t dbSNP:140655878

Target ORF information:

RefSeq Version NM_001029871
Organism Homo sapiens (human)
Definition Homo sapiens R-spondin 4 (RSPO4), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001029871

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGCGGGCGC CACTCTGCCT GCTCCTGCTC GTCGCCCACG CCGTGGACAT GCTCGCCCTG 
AACCGAAGGA AGAAGCAAGT GGGCACTGGC CTGGGGGGCA ACTGCACAGG CTGTATCATC
TGCTCAGAGG AGAACGGCTG TTCCACCTGC CAGCAGAGGC TCTTCCTGTT CATCCGCCGG
GAAGGCATCC GCCAGTACGG CAAGTGCCTG CACGACTGTC CCCCTGGGTA CTTCGGCATC
CGCGGCCAGG AGGTCAACAG GTGCAAAAAA TGTGGGGCCA CTTGTGAGAG CTGCTTCAGC
CAGGACTTCT GCATCCGGTG CAAGAGGCAG TTTTACTTGT ACAAGGGGAA GTGTCTGCCC
ACCTGCCCGC CGGGCACTTT GGCCCACCAG AACACACGGG AGTGCCAGGG GGAGTGTGAA
CTGGGTCCCT GGGGCGGCTG GAGCCCCTGC ACACACAATG GAAAGACCTG CGGCTCGGCT
TGGGGCCTGG AGAGCCGGGT ACGAGAGGCT GGCCGGGCTG GGCATGAGGA GGCAGCCACC
TGCCAGGTGC TTTCTGAGTC AAGGAAATGT CCCATCCAGA GGCCCTGCCC AGGAGAGAGG
AGCCCCGGCC AGAAGAAGGG CAGGAAGGAC CGGCGCCCAC GCAAGGACAG GAAGCTGGAC
CGCAGGCTGG ACGTGAGGCC GCGCCAGCCC GGCCTGCAGC CCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00129
Accession Version NM_001040007.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product R-spondin-4 isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ355152.1, AL050325.20 and BQ185331.1. On Sep 29, 2009 this sequence version replaced gi:90970327. Summary: This gene encodes a member of the R-spondin family of proteins that share a common domain organization consisting of a signal peptide, cysteine-rich/furin-like domain, thrombospondin domain and a C-terminal basic region. The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated with anonychia congenital. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009]. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: DQ355152.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142363, SAMEA2147920 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGCGGGCGC CACTCTGCCT GCTCCTGCTC GTCGCCCACG CCGTGGACAT GCTCGCCCTG 
AACCGAAGGA AGAAGCAAGT GGGCACTGGC CTGGGGGGCA ACTGCACAGG CTGTATCATC
TGCTCAGAGG AGAACGGCTG TTCCACCTGC CAGCAGAGGC TCTTCCTGTT CATCCGCCGG
GAAGGCATCC GCCAGTACGG CAAGTGCCTG CACGACTGTC CCCCTGGGTA CTTCGGCATC
CGCGGCCAGG AGGTCAACAG GTGCAAAAAA TGTGGGGCCA CTTGTGAGAG CTGCTTCAGC
CAGGACTTCT GCATCCGGTG CAAGAGGCAG TTTTACTTGT ACAAGGGGAA GTGTCTGCCC
ACCTGCCCGC CGGGCACTTT GGCCCACCAG AACACACGGG AGTGCCAGGA GAGGAGCCCC
GGCCAGAAGA AGGGCAGGAA GGACCGGCGC CCACGCAAGG ACAGGAAGCT GGACCGCAGG
CTGGACGTGA GGCCGCGCCA GCCCGGCCTG CAGCCCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001035096.1
CDS101..619
Misc Feature(1)353..484(+)
Misc Feature(2)368..508(+)
Exon (1)1..179
Gene:RSPO4
Gene Synonym:
Exon (2)180..368
Gene:RSPO4
Gene Synonym:
Exon (3)369..509
Gene:RSPO4
Gene Synonym:
Exon (4)510..2524
Gene:RSPO4
Gene Synonym:
Translation
Position Chain Variation Link
5 5 a, g dbSNP:552497905
13 13 c, g dbSNP:112044127
16 16 a, c, t dbSNP:6056520
62 62 a, g dbSNP:560184549
65 65 c, t dbSNP:768594197
70 70 c, g dbSNP:749426265
84 84 c, g dbSNP:780311755
90 90 c, t dbSNP:756348305
101 101 a, t dbSNP:746093558
107 107 a, g dbSNP:781688494
112 112 a, c dbSNP:150446609
130 130 c, t dbSNP:751990801
134 134 a, g dbSNP:373126184
140 140 a, g, t dbSNP:200584918
142 142 c, t dbSNP:6086831
143 143 a, g dbSNP:752157295
148 148 c, t dbSNP:530215228
151 151 g, t dbSNP:759118523
158 158 c, g dbSNP:776329354
163 163 a, c dbSNP:766071797
169 169 a, g dbSNP:760566194
173 173 a, c dbSNP:772989424
175 175 a, g dbSNP:772005883
176 176 c, g dbSNP:761856917
186 186 c, g dbSNP:761838651
193 193 g, t dbSNP:774489748
194 194 a, g dbSNP:199528639
198 198 -, g dbSNP:768138495
207 207 c, g, t dbSNP:776608135
211 211 a, c dbSNP:771139553
213 213 a, g dbSNP:377060056
214 214 c, t dbSNP:747212538
215 215 a, g dbSNP:763333338
221 221 c, t dbSNP:778130157
226 226 a, g dbSNP:550978379
227 227 a, g dbSNP:748638528
229 229 a, g dbSNP:201142150
230 230 a, g dbSNP:753113241
234 234 a, g dbSNP:753303855
236 236 a, g dbSNP:375035609
241 241 g, t dbSNP:755726509
245 245 a, c dbSNP:201933377
253 253 a, g dbSNP:767332107
255 255 a, g dbSNP:761750744
259 259 c, g, t dbSNP:371949198
261 261 a, t dbSNP:764084804
262 262 c, g dbSNP:763011320
265 265 c, g dbSNP:776717734
266 266 c, t dbSNP:766370306
268 268 a, g dbSNP:760943314
271 271 c, t dbSNP:773361802
272 272 a, g dbSNP:200792773
274 274 c, t dbSNP:748550698
275 275 c, t dbSNP:199844911
278 278 c, t dbSNP:769102112
284 284 a, g dbSNP:372674964
290 290 c, t dbSNP:387907027
291 291 a, g dbSNP:755637414
292 292 a, c dbSNP:745514631
294 294 a, g dbSNP:74315420
296 296 a, t dbSNP:757004626
298 298 c, t dbSNP:751352700
299 299 c, g dbSNP:387907028
300 300 g, t dbSNP:763998727
302 302 a, g dbSNP:758348841
303 303 a, t dbSNP:752640647
306 306 g, t dbSNP:766465255
307 307 c, t dbSNP:760715487
311 311 a, c dbSNP:773450068
314 314 a, c, g dbSNP:368559955
316 316 c, t dbSNP:762043573
318 318 a, g dbSNP:74315423
320 320 a, c, t dbSNP:769012270
322 322 c, t dbSNP:749789537
323 323 a, c dbSNP:775912164
334 334 c, t dbSNP:148789929
335 335 a, g dbSNP:542994015
342 342 a, g dbSNP:780874153
343 343 c, t dbSNP:756916549
344 344 a, c, g, t dbSNP:201062485
349 349 a, g dbSNP:574261920
358 358 c, t dbSNP:752655381
361 361 a, g dbSNP:765124151
365 365 a, g dbSNP:370469988
372 372 a, g dbSNP:765642242
373 373 -, tgg dbSNP:777661973
384 384 g, t dbSNP:780506366
385 385 c, t dbSNP:777330925
388 388 c, g dbSNP:770539145
395 395 g, t dbSNP:763385829
401 401 c, t dbSNP:387907026
402 402 a, g dbSNP:760299043
404 404 a, g dbSNP:772877858
405 405 a, c, g dbSNP:529579675
415 415 c, t dbSNP:778504126
416 416 c, t dbSNP:202132004
417 417 a, g dbSNP:6140807
419 419 c, t dbSNP:74315421
426 426 c, g dbSNP:755441249
427 427 a, g dbSNP:751592558
434 434 c, t dbSNP:377288758
438 438 c, t dbSNP:574845619
439 439 a, g dbSNP:753033719
442 442 c, t dbSNP:765511134
448 448 a, g dbSNP:755439339
453 453 a, g dbSNP:74315422
454 454 c, t dbSNP:564534973
459 459 c, t dbSNP:200597556
460 460 c, t dbSNP:199861662
467 467 c, g dbSNP:201485021
468 468 c, t dbSNP:370909673
469 469 a, g dbSNP:184279006
471 471 c, t dbSNP:774111255
472 472 a, g dbSNP:77472961
473 473 a, g dbSNP:369045323
474 474 a, g dbSNP:573862295
477 477 c, t dbSNP:201112120
478 478 g, t dbSNP:745812419
484 484 c, t dbSNP:777713947
496 496 a, t dbSNP:758567274
497 497 c, t dbSNP:200568022
498 498 a, g dbSNP:779231306
499 499 a, g dbSNP:571711499
509 509 a, g dbSNP:373277668
521 521 a, g dbSNP:755822660
534 534 a, g dbSNP:750184862
545 545 c, t dbSNP:767423520
546 546 a, g dbSNP:757255412
548 548 c, t dbSNP:202178299
549 549 a, g dbSNP:765335066
552 552 c, t dbSNP:374130199
554 554 c, t dbSNP:369687640
555 555 a, g dbSNP:377200892
565 565 a, g dbSNP:760903291
566 566 a, c dbSNP:773501751
575 575 c, t dbSNP:772494608
576 576 a, g dbSNP:762295741
580 580 g, t dbSNP:774771039
586 586 c, t dbSNP:759063553
587 587 c, g dbSNP:768182696
594 594 a, c, t dbSNP:201162335
595 595 a, g dbSNP:369860447
596 596 c, t dbSNP:745580942
597 597 a, g dbSNP:781119266
603 603 c, t dbSNP:375728608
604 604 c, t dbSNP:751507935
605 605 a, g dbSNP:563843176
621 621 c, t dbSNP:113093200
629 629 c, g dbSNP:534275132
632 632 -, ccgactctc dbSNP:573730556
633 633 a, c dbSNP:766487997
652 652 c, t dbSNP:747967944
655 655 a, g dbSNP:565675207
656 656 a, g dbSNP:767846748
661 661 c, t dbSNP:6118370
676 676 a, g dbSNP:549138091
762 762 a, g dbSNP:376648133
768 768 a, c dbSNP:563500394
776 776 c, g dbSNP:577512032
805 805 g, t dbSNP:549684296
806 806 c, t dbSNP:34773751
845 845 -, ttcc dbSNP:771985596
923 923 c, t dbSNP:564186113
953 953 c, t dbSNP:76811155
982 982 a, g dbSNP:184811687
984 984 a, g dbSNP:561739169
988 988 c, t dbSNP:192551017
1036 1036 a, g dbSNP:576106571
1062 1062 c, t dbSNP:534453835
1105 1105 a, g dbSNP:519478
1106 1106 c, t dbSNP:539605581
1112 1112 a, c dbSNP:140578124
1115 1115 a, g dbSNP:554214124
1116 1116 a, g dbSNP:776663727
1123 1123 c, g dbSNP:755620946
1155 1155 c, t dbSNP:534495204
1159 1159 a, g dbSNP:745363996
1179 1179 c, t dbSNP:565712072
1181 1181 a, g dbSNP:543936481
1183 1183 g, t dbSNP:778168615
1203 1203 g, t dbSNP:147214985
1219 1219 c, t dbSNP:569735482
1236 1236 c, t dbSNP:549960964
1256 1256 a, g dbSNP:369505776
1257 1257 c, t dbSNP:375151240
1262 1262 a, g dbSNP:547417155
1304 1304 a, g dbSNP:372460184
1307 1307 a, g dbSNP:527566729
1376 1376 c, g dbSNP:561775766
1382 1382 c, t dbSNP:149543960
1387 1387 c, t dbSNP:187647199
1404 1404 c, g dbSNP:562793850
1407 1407 c, t dbSNP:13037150
1413 1413 c, g dbSNP:516714
1424 1424 a, c dbSNP:755386404
1437 1437 c, t dbSNP:554325646
1442 1442 c, g dbSNP:138721103
1447 1447 g, t dbSNP:754044098
1461 1461 a, g dbSNP:575155829
1501 1501 a, g dbSNP:182220957
1508 1508 a, g dbSNP:62187523
1512 1512 a, c dbSNP:552629982
1564 1564 g, t dbSNP:750474691
1593 1593 a, g dbSNP:767445596
1612 1612 a, g dbSNP:556033593
1631 1631 a, g dbSNP:539511973
1659 1659 a, g dbSNP:570590295
1661 1661 c, t dbSNP:509384
1685 1685 c, t dbSNP:527602996
1692 1692 a, g dbSNP:567972603
1721 1721 c, t dbSNP:150625916
1742 1742 a, g dbSNP:190629434
1755 1755 a, c dbSNP:79127824
1763 1763 c, t dbSNP:530194832
1771 1771 c, t dbSNP:529913520
1783 1783 -, tc dbSNP:200388392
1797 1797 a, c dbSNP:76535756
1801 1801 c, t dbSNP:560829312
1805 1805 c, t dbSNP:6108208
1817 1817 c, t dbSNP:62187522
1822 1822 a, g dbSNP:62187521
1841 1841 a, c dbSNP:62187520
1849 1849 c, g dbSNP:6118367
1850 1850 c, g dbSNP:532614806
1863 1863 a, c dbSNP:62187519
1866 1866 a, g dbSNP:560539826
1883 1883 c, t dbSNP:62187518
1888 1888 a, g dbSNP:62187517
1894 1894 c, g dbSNP:58158356
1916 1916 g, t dbSNP:34935852
1947 1947 c, t dbSNP:188433140
1954 1954 a, g dbSNP:13043975
2019 2019 a, g dbSNP:944117
2023 2023 a, g dbSNP:734940
2046 2046 c, g dbSNP:3746813
2079 2079 c, t dbSNP:182992578
2096 2096 a, g dbSNP:944116
2116 2116 c, t dbSNP:553566865
2122 2122 a, g dbSNP:371322363
2138 2138 a, c dbSNP:115673250
2163 2163 c, t dbSNP:555226164
2164 2164 a, g dbSNP:567909164
2184 2184 g, t dbSNP:548118025
2208 2208 c, t dbSNP:531484592
2243 2243 a, g dbSNP:191999547
2293 2293 c, t dbSNP:552513871
2325 2325 c, g dbSNP:3746812
2326 2326 a, c, t dbSNP:574832275
2327 2327 -, c, ct dbSNP:58319408
2327 2327 a, c dbSNP:3746811
2328 2328 -, tc dbSNP:3833342
2328 2328 -, tc dbSNP:397840834
2331 2331 -, c dbSNP:200839031
2358 2358 c, t dbSNP:80086411
2374 2374 c, t dbSNP:377701364
2389 2389 a, c dbSNP:575873978
2400 2400 a, g dbSNP:779528009
2402 2402 a, c dbSNP:562367020
2433 2433 c, g, t dbSNP:569229471
2445 2445 c, t dbSNP:576867453
2453 2453 c, t dbSNP:486347
2455 2455 c, t dbSNP:752576473
2456 2456 a, g dbSNP:373290716
2458 2458 c, t dbSNP:486344
2471 2471 a, g dbSNP:554318960
2474 2474 c, t dbSNP:756291872
2488 2488 a, t dbSNP:554753373
2488 2488 -, t dbSNP:780482661
2489 2489 a, t dbSNP:480012
2492 2492 -, a dbSNP:369731512
2492 2492 a, t dbSNP:552295984
2513 2513 g, t dbSNP:140655878

Target ORF information:

RefSeq Version NM_001040007
Organism Homo sapiens (human)
Definition Homo sapiens R-spondin 4 (RSPO4), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001040007

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGCGGGCGC CACTCTGCCT GCTCCTGCTC GTCGCCCACG CCGTGGACAT GCTCGCCCTG 
AACCGAAGGA AGAAGCAAGT GGGCACTGGC CTGGGGGGCA ACTGCACAGG CTGTATCATC
TGCTCAGAGG AGAACGGCTG TTCCACCTGC CAGCAGAGGC TCTTCCTGTT CATCCGCCGG
GAAGGCATCC GCCAGTACGG CAAGTGCCTG CACGACTGTC CCCCTGGGTA CTTCGGCATC
CGCGGCCAGG AGGTCAACAG GTGCAAAAAA TGTGGGGCCA CTTGTGAGAG CTGCTTCAGC
CAGGACTTCT GCATCCGGTG CAAGAGGCAG TTTTACTTGT ACAAGGGGAA GTGTCTGCCC
ACCTGCCCGC CGGGCACTTT GGCCCACCAG AACACACGGG AGTGCCAGGA GAGGAGCCCC
GGCCAGAAGA AGGGCAGGAA GGACCGGCGC CCACGCAAGG ACAGGAAGCT GGACCGCAGG
CTGGACGTGA GGCCGCGCCA GCCCGGCCTG CAGCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.