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APOE cDNA ORF clone, Homo sapiens (human)

Gene Symbol APOE
Entrez Gene ID 348
Full Name apolipoprotein E
Synonyms AD2, APO-E, LDLCQ5, LPG
General protein information
Preferred Names
apolipoprotein E
Names
apolipoprotein E
apo-E
apolipoprotein E3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19q13.2

Summary The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]. lac of sum
Disorder MIM:

107741

Disorder Html: Hyperlipoproteinemia, type III (3); {Myocardial infarction

mRNA and Protein(s)

mRNA Protein Name
NM_001302688 NP_001289617 apolipoprotein E isoform a precursor
NM_000041 NP_000032 apolipoprotein E isoform b precursor
NM_001302691 NP_001289620 apolipoprotein E isoform b precursor
NM_001302690 NP_001289619 apolipoprotein E isoform b precursor
NM_001302689 NP_001289618 apolipoprotein E isoform b precursor



Homo sapiens (human) APOE NP_000032.1
Pan troglodytes (chimpanzee) APOE NP_001009007.1
Macaca mulatta (Rhesus monkey) APOE XP_001104482.2
Canis lupus familiaris (dog) APOE XP_866043.1
Bos taurus (cattle) APOE NP_776416.1
Mus musculus (house mouse) Apoe NP_033826.2
Rattus norvegicus (Norway rat) Apoe NP_620183.2
Xenopus (Silurana) tropicalis (western clawed frog) apoe XP_004912063.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following APOE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APOE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu52233 NM_001302688 Homo sapiens apolipoprotein E (APOE), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00
OHu27296
NM_000041 Homo sapiens apolipoprotein E (APOE), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu27296
NM_001302691 Homo sapiens apolipoprotein E (APOE), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu27296
NM_001302690 Homo sapiens apolipoprotein E (APOE), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu27296
NM_001302689 Homo sapiens apolipoprotein E (APOE), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu52233
Clone ID Related Accession (Same CDS sequence) NM_001302688
Accession Version NM_001302688.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1032bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product apolipoprotein E isoform a precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY056112.1, BM042032.1, BC003557.1 and AL037046.3. On Nov 7, 2014 this sequence version replaced gi:530416440. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI602495.1, BU190668.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGCTCAG GGGCCTCTAG AAAGAGCTGG GACCCTGGGA ACCCCTGGCC TCCAGACTGG 
CCAATCACAG GCAGGAAGAT GAAGGTTCTG TGGGCTGCGT TGCTGGTCAC ATTCCTGGCA
GGATGCCAGG CCAAGGTGGA GCAAGCGGTG GAGACAGAGC CGGAGCCCGA GCTGCGCCAG
CAGACCGAGT GGCAGAGCGG CCAGCGCTGG GAACTGGCAC TGGGTCGCTT TTGGGATTAC
CTGCGCTGGG TGCAGACACT GTCTGAGCAG GTGCAGGAGG AGCTGCTCAG CTCCCAGGTC
ACCCAGGAAC TGAGGGCGCT GATGGACGAG ACCATGAAGG AGTTGAAGGC CTACAAATCG
GAACTGGAGG AACAACTGAC CCCGGTGGCG GAGGAGACGC GGGCACGGCT GTCCAAGGAG
CTGCAGGCGG CGCAGGCCCG GCTGGGCGCG GACATGGAGG ACGTGTGCGG CCGCCTGGTG
CAGTACCGCG GCGAGGTGCA GGCCATGCTC GGCCAGAGCA CCGAGGAGCT GCGGGTGCGC
CTCGCCTCCC ACCTGCGCAA GCTGCGTAAG CGGCTCCTCC GCGATGCCGA TGACCTGCAG
AAGCGCCTGG CAGTGTACCA GGCCGGGGCC CGCGAGGGCG CCGAGCGCGG CCTCAGCGCC
ATCCGCGAGC GCCTGGGGCC CCTGGTGGAA CAGGGCCGCG TGCGGGCCGC CACTGTGGGC
TCCCTGGCCG GCCAGCCGCT ACAGGAGCGG GCCCAGGCCT GGGGCGAGCG GCTGCGCGCG
CGGATGGAGG AGATGGGCAG CCGGACCCGC GACCGCCTGG ACGAGGTGAA GGAGCAGGTG
GCGGAGGTGC GCGCCAAGCT GGAGGAGCAG GCCCAGCAGA TACGCCTGCA GGCCGAGGCC
TTCCAGGCCC GCCTCAAGAG CTGGTTCGAG CCCCTGGTGG AAGACATGCA GCGCCAGTGG
GCCGGGCTGG TGGAGAAGGT GCAGGCTGCC GTGGGCACCA GCGCCGCCCC TGTGCCCAGC
GACAATCACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001289617.1
CDS121..1152
Misc Feature(1)19..21(+)
Misc Feature(2)508..1095(+)
Exon (1)1..175
Gene:APOE
Gene Synonym:
Exon (2)176..241
Gene:APOE
Gene Synonym:
Exon (3)242..434
Gene:APOE
Gene Synonym:
Exon (4)435..1297
Gene:APOE
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001302688
Organism Homo sapiens (human)
Definition Homo sapiens apolipoprotein E (APOE), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001302688

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGCTCAG GGGCCTCTAG AAAGAGCTGG GACCCTGGGA ACCCCTGGCC TCCAGACTGG 
CCAATCACAG GCAGGAAGAT GAAGGTTCTG TGGGCTGCGT TGCTGGTCAC ATTCCTGGCA
GGATGCCAGG CCAAGGTGGA GCAAGCGGTG GAGACAGAGC CGGAGCCCGA GCTGCGCCAG
CAGACCGAGT GGCAGAGCGG CCAGCGCTGG GAACTGGCAC TGGGTCGCTT TTGGGATTAC
CTGCGCTGGG TGCAGACACT GTCTGAGCAG GTGCAGGAGG AGCTGCTCAG CTCCCAGGTC
ACCCAGGAAC TGAGGGCGCT GATGGACGAG ACCATGAAGG AGTTGAAGGC CTACAAATCG
GAACTGGAGG AACAACTGAC CCCGGTGGCG GAGGAGACGC GGGCACGGCT GTCCAAGGAG
CTGCAGGCGG CGCAGGCCCG GCTGGGCGCG GACATGGAGG ACGTGTGCGG CCGCCTGGTG
CAGTACCGCG GCGAGGTGCA GGCCATGCTC GGCCAGAGCA CCGAGGAGCT GCGGGTGCGC
CTCGCCTCCC ACCTGCGCAA GCTGCGTAAG CGGCTCCTCC GCGATGCCGA TGACCTGCAG
AAGCGCCTGG CAGTGTACCA GGCCGGGGCC CGCGAGGGCG CCGAGCGCGG CCTCAGCGCC
ATCCGCGAGC GCCTGGGGCC CCTGGTGGAA CAGGGCCGCG TGCGGGCCGC CACTGTGGGC
TCCCTGGCCG GCCAGCCGCT ACAGGAGCGG GCCCAGGCCT GGGGCGAGCG GCTGCGCGCG
CGGATGGAGG AGATGGGCAG CCGGACCCGC GACCGCCTGG ACGAGGTGAA GGAGCAGGTG
GCGGAGGTGC GCGCCAAGCT GGAGGAGCAG GCCCAGCAGA TACGCCTGCA GGCCGAGGCC
TTCCAGGCCC GCCTCAAGAG CTGGTTCGAG CCCCTGGTGG AAGACATGCA GCGCCAGTGG
GCCGGGCTGG TGGAGAAGGT GCAGGCTGCC GTGGGCACCA GCGCCGCCCC TGTGCCCAGC
GACAATCACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27296
Clone ID Related Accession (Same CDS sequence) NM_000041 , NM_001302689 , NM_001302690 , NM_001302691
Accession Version NM_000041.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product apolipoprotein E isoform b precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY056112.1, BC003557.1 and AL037046.3. This sequence is a reference standard in the RefSeqGene project. On Nov 7, 2014 this sequence version replaced gi:48762938. Transcript Variant: This variant (2) contains an alternate 5' terminal exon, and it thus differs in the 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Variants 2, 3, 4 and 5 all encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY036369.1, BC003557.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGGTTC TGTGGGCTGC GTTGCTGGTC ACATTCCTGG CAGGATGCCA GGCCAAGGTG 
GAGCAAGCGG TGGAGACAGA GCCGGAGCCC GAGCTGCGCC AGCAGACCGA GTGGCAGAGC
GGCCAGCGCT GGGAACTGGC ACTGGGTCGC TTTTGGGATT ACCTGCGCTG GGTGCAGACA
CTGTCTGAGC AGGTGCAGGA GGAGCTGCTC AGCTCCCAGG TCACCCAGGA ACTGAGGGCG
CTGATGGACG AGACCATGAA GGAGTTGAAG GCCTACAAAT CGGAACTGGA GGAACAACTG
ACCCCGGTGG CGGAGGAGAC GCGGGCACGG CTGTCCAAGG AGCTGCAGGC GGCGCAGGCC
CGGCTGGGCG CGGACATGGA GGACGTGTGC GGCCGCCTGG TGCAGTACCG CGGCGAGGTG
CAGGCCATGC TCGGCCAGAG CACCGAGGAG CTGCGGGTGC GCCTCGCCTC CCACCTGCGC
AAGCTGCGTA AGCGGCTCCT CCGCGATGCC GATGACCTGC AGAAGCGCCT GGCAGTGTAC
CAGGCCGGGG CCCGCGAGGG CGCCGAGCGC GGCCTCAGCG CCATCCGCGA GCGCCTGGGG
CCCCTGGTGG AACAGGGCCG CGTGCGGGCC GCCACTGTGG GCTCCCTGGC CGGCCAGCCG
CTACAGGAGC GGGCCCAGGC CTGGGGCGAG CGGCTGCGCG CGCGGATGGA GGAGATGGGC
AGCCGGACCC GCGACCGCCT GGACGAGGTG AAGGAGCAGG TGGCGGAGGT GCGCGCCAAG
CTGGAGGAGC AGGCCCAGCA GATACGCCTG CAGGCCGAGG CCTTCCAGGC CCGCCTCAAG
AGCTGGTTCG AGCCCCTGGT GGAAGACATG CAGCGCCAGT GGGCCGGGCT GGTGGAGAAG
GTGCAGGCTG CCGTGGGCAC CAGCGCCGCC CCTGTGCCCA GCGACAATCA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000032.1
CDS117..1070
Misc Feature(1)42..44(+)
Misc Feature(2)354..881(+)
Misc Feature(3)393..395(+)
Misc Feature(4)426..1013(+)
Misc Feature(5)543..545(+)
Misc Feature(6)588..620(+)
Misc Feature(7)600..611(+)
Misc Feature(8)750..752(+)
Misc Feature(9)801..824(+)
Misc Feature(10)972..974(+)
Misc Feature(11)984..986(+)
Exon (1)1..93
Gene:APOE
Gene Synonym:
Exon (2)94..159
Gene:APOE
Gene Synonym:
Exon (3)160..352
Gene:APOE
Gene Synonym:
Exon (4)353..1215
Gene:APOE
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000041
Organism Homo sapiens (human)
Definition Homo sapiens apolipoprotein E (APOE), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000041

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGGTTC TGTGGGCTGC GTTGCTGGTC ACATTCCTGG CAGGATGCCA GGCCAAGGTG 
GAGCAAGCGG TGGAGACAGA GCCGGAGCCC GAGCTGCGCC AGCAGACCGA GTGGCAGAGC
GGCCAGCGCT GGGAACTGGC ACTGGGTCGC TTTTGGGATT ACCTGCGCTG GGTGCAGACA
CTGTCTGAGC AGGTGCAGGA GGAGCTGCTC AGCTCCCAGG TCACCCAGGA ACTGAGGGCG
CTGATGGACG AGACCATGAA GGAGTTGAAG GCCTACAAAT CGGAACTGGA GGAACAACTG
ACCCCGGTGG CGGAGGAGAC GCGGGCACGG CTGTCCAAGG AGCTGCAGGC GGCGCAGGCC
CGGCTGGGCG CGGACATGGA GGACGTGTGC GGCCGCCTGG TGCAGTACCG CGGCGAGGTG
CAGGCCATGC TCGGCCAGAG CACCGAGGAG CTGCGGGTGC GCCTCGCCTC CCACCTGCGC
AAGCTGCGTA AGCGGCTCCT CCGCGATGCC GATGACCTGC AGAAGCGCCT GGCAGTGTAC
CAGGCCGGGG CCCGCGAGGG CGCCGAGCGC GGCCTCAGCG CCATCCGCGA GCGCCTGGGG
CCCCTGGTGG AACAGGGCCG CGTGCGGGCC GCCACTGTGG GCTCCCTGGC CGGCCAGCCG
CTACAGGAGC GGGCCCAGGC CTGGGGCGAG CGGCTGCGCG CGCGGATGGA GGAGATGGGC
AGCCGGACCC GCGACCGCCT GGACGAGGTG AAGGAGCAGG TGGCGGAGGT GCGCGCCAAG
CTGGAGGAGC AGGCCCAGCA GATACGCCTG CAGGCCGAGG CCTTCCAGGC CCGCCTCAAG
AGCTGGTTCG AGCCCCTGGT GGAAGACATG CAGCGCCAGT GGGCCGGGCT GGTGGAGAAG
GTGCAGGCTG CCGTGGGCAC CAGCGCCGCC CCTGTGCCCA GCGACAATCA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27296
Clone ID Related Accession (Same CDS sequence) NM_000041 , NM_001302689 , NM_001302690 , NM_001302691
Accession Version NM_001302691.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product apolipoprotein E isoform b precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY056112.1, BI550631.1, BC003557.1 and AL037046.3. Transcript Variant: This variant (5) contains an alternate 5' terminal exon and uses an alternate splice site in another 5' exon, and it thus differs in the 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Variants 2, 3, 4 and 5 all encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI550631.1, BI670331.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGGTTC TGTGGGCTGC GTTGCTGGTC ACATTCCTGG CAGGATGCCA GGCCAAGGTG 
GAGCAAGCGG TGGAGACAGA GCCGGAGCCC GAGCTGCGCC AGCAGACCGA GTGGCAGAGC
GGCCAGCGCT GGGAACTGGC ACTGGGTCGC TTTTGGGATT ACCTGCGCTG GGTGCAGACA
CTGTCTGAGC AGGTGCAGGA GGAGCTGCTC AGCTCCCAGG TCACCCAGGA ACTGAGGGCG
CTGATGGACG AGACCATGAA GGAGTTGAAG GCCTACAAAT CGGAACTGGA GGAACAACTG
ACCCCGGTGG CGGAGGAGAC GCGGGCACGG CTGTCCAAGG AGCTGCAGGC GGCGCAGGCC
CGGCTGGGCG CGGACATGGA GGACGTGTGC GGCCGCCTGG TGCAGTACCG CGGCGAGGTG
CAGGCCATGC TCGGCCAGAG CACCGAGGAG CTGCGGGTGC GCCTCGCCTC CCACCTGCGC
AAGCTGCGTA AGCGGCTCCT CCGCGATGCC GATGACCTGC AGAAGCGCCT GGCAGTGTAC
CAGGCCGGGG CCCGCGAGGG CGCCGAGCGC GGCCTCAGCG CCATCCGCGA GCGCCTGGGG
CCCCTGGTGG AACAGGGCCG CGTGCGGGCC GCCACTGTGG GCTCCCTGGC CGGCCAGCCG
CTACAGGAGC GGGCCCAGGC CTGGGGCGAG CGGCTGCGCG CGCGGATGGA GGAGATGGGC
AGCCGGACCC GCGACCGCCT GGACGAGGTG AAGGAGCAGG TGGCGGAGGT GCGCGCCAAG
CTGGAGGAGC AGGCCCAGCA GATACGCCTG CAGGCCGAGG CCTTCCAGGC CCGCCTCAAG
AGCTGGTTCG AGCCCCTGGT GGAAGACATG CAGCGCCAGT GGGCCGGGCT GGTGGAGAAG
GTGCAGGCTG CCGTGGGCAC CAGCGCCGCC CCTGTGCCCA GCGACAATCA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001289620.1
CDS132..1085
Misc Feature(1)42..44(+)
Misc Feature(2)369..896(+)
Misc Feature(3)441..1028(+)
Misc Feature(4)558..560(+)
Misc Feature(5)570..572(+)
Misc Feature(6)603..635(+)
Misc Feature(7)615..626(+)
Misc Feature(8)816..839(+)
Exon (1)1..93
Gene:APOE
Gene Synonym:
Exon (2)94..174
Gene:APOE
Gene Synonym:
Exon (3)175..367
Gene:APOE
Gene Synonym:
Exon (4)368..1230
Gene:APOE
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001302691
Organism Homo sapiens (human)
Definition Homo sapiens apolipoprotein E (APOE), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001302691

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGGTTC TGTGGGCTGC GTTGCTGGTC ACATTCCTGG CAGGATGCCA GGCCAAGGTG 
GAGCAAGCGG TGGAGACAGA GCCGGAGCCC GAGCTGCGCC AGCAGACCGA GTGGCAGAGC
GGCCAGCGCT GGGAACTGGC ACTGGGTCGC TTTTGGGATT ACCTGCGCTG GGTGCAGACA
CTGTCTGAGC AGGTGCAGGA GGAGCTGCTC AGCTCCCAGG TCACCCAGGA ACTGAGGGCG
CTGATGGACG AGACCATGAA GGAGTTGAAG GCCTACAAAT CGGAACTGGA GGAACAACTG
ACCCCGGTGG CGGAGGAGAC GCGGGCACGG CTGTCCAAGG AGCTGCAGGC GGCGCAGGCC
CGGCTGGGCG CGGACATGGA GGACGTGTGC GGCCGCCTGG TGCAGTACCG CGGCGAGGTG
CAGGCCATGC TCGGCCAGAG CACCGAGGAG CTGCGGGTGC GCCTCGCCTC CCACCTGCGC
AAGCTGCGTA AGCGGCTCCT CCGCGATGCC GATGACCTGC AGAAGCGCCT GGCAGTGTAC
CAGGCCGGGG CCCGCGAGGG CGCCGAGCGC GGCCTCAGCG CCATCCGCGA GCGCCTGGGG
CCCCTGGTGG AACAGGGCCG CGTGCGGGCC GCCACTGTGG GCTCCCTGGC CGGCCAGCCG
CTACAGGAGC GGGCCCAGGC CTGGGGCGAG CGGCTGCGCG CGCGGATGGA GGAGATGGGC
AGCCGGACCC GCGACCGCCT GGACGAGGTG AAGGAGCAGG TGGCGGAGGT GCGCGCCAAG
CTGGAGGAGC AGGCCCAGCA GATACGCCTG CAGGCCGAGG CCTTCCAGGC CCGCCTCAAG
AGCTGGTTCG AGCCCCTGGT GGAAGACATG CAGCGCCAGT GGGCCGGGCT GGTGGAGAAG
GTGCAGGCTG CCGTGGGCAC CAGCGCCGCC CCTGTGCCCA GCGACAATCA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27296
Clone ID Related Accession (Same CDS sequence) NM_000041 , NM_001302689 , NM_001302690 , NM_001302691
Accession Version NM_001302690.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product apolipoprotein E isoform b precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CA771535.1, HY074888.1, BC003557.1 and AL037046.3. Transcript Variant: This variant (4) contains an alternate 5' terminal exon, and it thus differs in the 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Variants 2, 3, 4 and 5 all encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY074888.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145122 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGGTTC TGTGGGCTGC GTTGCTGGTC ACATTCCTGG CAGGATGCCA GGCCAAGGTG 
GAGCAAGCGG TGGAGACAGA GCCGGAGCCC GAGCTGCGCC AGCAGACCGA GTGGCAGAGC
GGCCAGCGCT GGGAACTGGC ACTGGGTCGC TTTTGGGATT ACCTGCGCTG GGTGCAGACA
CTGTCTGAGC AGGTGCAGGA GGAGCTGCTC AGCTCCCAGG TCACCCAGGA ACTGAGGGCG
CTGATGGACG AGACCATGAA GGAGTTGAAG GCCTACAAAT CGGAACTGGA GGAACAACTG
ACCCCGGTGG CGGAGGAGAC GCGGGCACGG CTGTCCAAGG AGCTGCAGGC GGCGCAGGCC
CGGCTGGGCG CGGACATGGA GGACGTGTGC GGCCGCCTGG TGCAGTACCG CGGCGAGGTG
CAGGCCATGC TCGGCCAGAG CACCGAGGAG CTGCGGGTGC GCCTCGCCTC CCACCTGCGC
AAGCTGCGTA AGCGGCTCCT CCGCGATGCC GATGACCTGC AGAAGCGCCT GGCAGTGTAC
CAGGCCGGGG CCCGCGAGGG CGCCGAGCGC GGCCTCAGCG CCATCCGCGA GCGCCTGGGG
CCCCTGGTGG AACAGGGCCG CGTGCGGGCC GCCACTGTGG GCTCCCTGGC CGGCCAGCCG
CTACAGGAGC GGGCCCAGGC CTGGGGCGAG CGGCTGCGCG CGCGGATGGA GGAGATGGGC
AGCCGGACCC GCGACCGCCT GGACGAGGTG AAGGAGCAGG TGGCGGAGGT GCGCGCCAAG
CTGGAGGAGC AGGCCCAGCA GATACGCCTG CAGGCCGAGG CCTTCCAGGC CCGCCTCAAG
AGCTGGTTCG AGCCCCTGGT GGAAGACATG CAGCGCCAGT GGGCCGGGCT GGTGGAGAAG
GTGCAGGCTG CCGTGGGCAC CAGCGCCGCC CCTGTGCCCA GCGACAATCA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001289619.1
CDS148..1101
Misc Feature(1)79..81(+)
Misc Feature(2)385..912(+)
Misc Feature(3)457..1044(+)
Misc Feature(4)574..576(+)
Misc Feature(5)586..588(+)
Misc Feature(6)619..651(+)
Misc Feature(7)631..642(+)
Misc Feature(8)832..855(+)
Exon (1)1..124
Gene:APOE
Gene Synonym:
Exon (2)125..190
Gene:APOE
Gene Synonym:
Exon (3)191..383
Gene:APOE
Gene Synonym:
Exon (4)384..1246
Gene:APOE
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001302690
Organism Homo sapiens (human)
Definition Homo sapiens apolipoprotein E (APOE), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001302690

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGGTTC TGTGGGCTGC GTTGCTGGTC ACATTCCTGG CAGGATGCCA GGCCAAGGTG 
GAGCAAGCGG TGGAGACAGA GCCGGAGCCC GAGCTGCGCC AGCAGACCGA GTGGCAGAGC
GGCCAGCGCT GGGAACTGGC ACTGGGTCGC TTTTGGGATT ACCTGCGCTG GGTGCAGACA
CTGTCTGAGC AGGTGCAGGA GGAGCTGCTC AGCTCCCAGG TCACCCAGGA ACTGAGGGCG
CTGATGGACG AGACCATGAA GGAGTTGAAG GCCTACAAAT CGGAACTGGA GGAACAACTG
ACCCCGGTGG CGGAGGAGAC GCGGGCACGG CTGTCCAAGG AGCTGCAGGC GGCGCAGGCC
CGGCTGGGCG CGGACATGGA GGACGTGTGC GGCCGCCTGG TGCAGTACCG CGGCGAGGTG
CAGGCCATGC TCGGCCAGAG CACCGAGGAG CTGCGGGTGC GCCTCGCCTC CCACCTGCGC
AAGCTGCGTA AGCGGCTCCT CCGCGATGCC GATGACCTGC AGAAGCGCCT GGCAGTGTAC
CAGGCCGGGG CCCGCGAGGG CGCCGAGCGC GGCCTCAGCG CCATCCGCGA GCGCCTGGGG
CCCCTGGTGG AACAGGGCCG CGTGCGGGCC GCCACTGTGG GCTCCCTGGC CGGCCAGCCG
CTACAGGAGC GGGCCCAGGC CTGGGGCGAG CGGCTGCGCG CGCGGATGGA GGAGATGGGC
AGCCGGACCC GCGACCGCCT GGACGAGGTG AAGGAGCAGG TGGCGGAGGT GCGCGCCAAG
CTGGAGGAGC AGGCCCAGCA GATACGCCTG CAGGCCGAGG CCTTCCAGGC CCGCCTCAAG
AGCTGGTTCG AGCCCCTGGT GGAAGACATG CAGCGCCAGT GGGCCGGGCT GGTGGAGAAG
GTGCAGGCTG CCGTGGGCAC CAGCGCCGCC CCTGTGCCCA GCGACAATCA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27296
Clone ID Related Accession (Same CDS sequence) NM_000041 , NM_001302689 , NM_001302690 , NM_001302691
Accession Version NM_001302689.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product apolipoprotein E isoform b precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB486513.1, HY074384.1, BC003557.1 and AL037046.3. Transcript Variant: This variant (3) contains an alternate 5' terminal exon, and it thus differs in the 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Variants 2, 3, 4 and 5 all encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DB486513.1, HY075493.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGGTTC TGTGGGCTGC GTTGCTGGTC ACATTCCTGG CAGGATGCCA GGCCAAGGTG 
GAGCAAGCGG TGGAGACAGA GCCGGAGCCC GAGCTGCGCC AGCAGACCGA GTGGCAGAGC
GGCCAGCGCT GGGAACTGGC ACTGGGTCGC TTTTGGGATT ACCTGCGCTG GGTGCAGACA
CTGTCTGAGC AGGTGCAGGA GGAGCTGCTC AGCTCCCAGG TCACCCAGGA ACTGAGGGCG
CTGATGGACG AGACCATGAA GGAGTTGAAG GCCTACAAAT CGGAACTGGA GGAACAACTG
ACCCCGGTGG CGGAGGAGAC GCGGGCACGG CTGTCCAAGG AGCTGCAGGC GGCGCAGGCC
CGGCTGGGCG CGGACATGGA GGACGTGTGC GGCCGCCTGG TGCAGTACCG CGGCGAGGTG
CAGGCCATGC TCGGCCAGAG CACCGAGGAG CTGCGGGTGC GCCTCGCCTC CCACCTGCGC
AAGCTGCGTA AGCGGCTCCT CCGCGATGCC GATGACCTGC AGAAGCGCCT GGCAGTGTAC
CAGGCCGGGG CCCGCGAGGG CGCCGAGCGC GGCCTCAGCG CCATCCGCGA GCGCCTGGGG
CCCCTGGTGG AACAGGGCCG CGTGCGGGCC GCCACTGTGG GCTCCCTGGC CGGCCAGCCG
CTACAGGAGC GGGCCCAGGC CTGGGGCGAG CGGCTGCGCG CGCGGATGGA GGAGATGGGC
AGCCGGACCC GCGACCGCCT GGACGAGGTG AAGGAGCAGG TGGCGGAGGT GCGCGCCAAG
CTGGAGGAGC AGGCCCAGCA GATACGCCTG CAGGCCGAGG CCTTCCAGGC CCGCCTCAAG
AGCTGGTTCG AGCCCCTGGT GGAAGACATG CAGCGCCAGT GGGCCGGGCT GGTGGAGAAG
GTGCAGGCTG CCGTGGGCAC CAGCGCCGCC CCTGTGCCCA GCGACAATCA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001289618.1
CDS48..1001
Misc Feature(1)285..812(+)
Misc Feature(2)357..944(+)
Misc Feature(3)474..476(+)
Misc Feature(4)486..488(+)
Misc Feature(5)519..551(+)
Misc Feature(6)531..542(+)
Misc Feature(7)732..755(+)
Exon (1)1..24
Gene:APOE
Gene Synonym:
Exon (2)25..90
Gene:APOE
Gene Synonym:
Exon (3)91..283
Gene:APOE
Gene Synonym:
Exon (4)284..1146
Gene:APOE
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001302689
Organism Homo sapiens (human)
Definition Homo sapiens apolipoprotein E (APOE), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001302689

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGGTTC TGTGGGCTGC GTTGCTGGTC ACATTCCTGG CAGGATGCCA GGCCAAGGTG 
GAGCAAGCGG TGGAGACAGA GCCGGAGCCC GAGCTGCGCC AGCAGACCGA GTGGCAGAGC
GGCCAGCGCT GGGAACTGGC ACTGGGTCGC TTTTGGGATT ACCTGCGCTG GGTGCAGACA
CTGTCTGAGC AGGTGCAGGA GGAGCTGCTC AGCTCCCAGG TCACCCAGGA ACTGAGGGCG
CTGATGGACG AGACCATGAA GGAGTTGAAG GCCTACAAAT CGGAACTGGA GGAACAACTG
ACCCCGGTGG CGGAGGAGAC GCGGGCACGG CTGTCCAAGG AGCTGCAGGC GGCGCAGGCC
CGGCTGGGCG CGGACATGGA GGACGTGTGC GGCCGCCTGG TGCAGTACCG CGGCGAGGTG
CAGGCCATGC TCGGCCAGAG CACCGAGGAG CTGCGGGTGC GCCTCGCCTC CCACCTGCGC
AAGCTGCGTA AGCGGCTCCT CCGCGATGCC GATGACCTGC AGAAGCGCCT GGCAGTGTAC
CAGGCCGGGG CCCGCGAGGG CGCCGAGCGC GGCCTCAGCG CCATCCGCGA GCGCCTGGGG
CCCCTGGTGG AACAGGGCCG CGTGCGGGCC GCCACTGTGG GCTCCCTGGC CGGCCAGCCG
CTACAGGAGC GGGCCCAGGC CTGGGGCGAG CGGCTGCGCG CGCGGATGGA GGAGATGGGC
AGCCGGACCC GCGACCGCCT GGACGAGGTG AAGGAGCAGG TGGCGGAGGT GCGCGCCAAG
CTGGAGGAGC AGGCCCAGCA GATACGCCTG CAGGCCGAGG CCTTCCAGGC CCGCCTCAAG
AGCTGGTTCG AGCCCCTGGT GGAAGACATG CAGCGCCAGT GGGCCGGGCT GGTGGAGAAG
GTGCAGGCTG CCGTGGGCAC CAGCGCCGCC CCTGTGCCCA GCGACAATCA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Apolipoprotein E genotyping in women with recurrent pregnancy loss: an in silico and experimental hybrid study
Gene 549 (2), 209-213 (2014)
Poursadegh Zonouzi A, Farajzadeh D, Bargahi N and Farajzadeh M.


book

APOE polymorphisms and cognitive functions in patients with brain tumors
Neurology 83 (4), 320-327 (2014)
Correa DD, Satagopan J, Baser RE, Cheung K, Richards E, Lin M, Karimi S, Lyo J, DeAngelis LM and Orlow I.


book

An updated meta-analysis: apolipoprotein E genotypes and risk of primary open-angle glaucoma
Mol. Vis. 20, 1025-1036 (2014)
Liao R, Ye M and Xu X.


book

Gene-environment interactions: lifetime cognitive activity, APOE genotype, and beta-amyloid burden
J. Neurosci. 34 (25), 8612-8617 (2014)
Wirth M, Villeneuve S, La Joie R, Marks SM and Jagust WJ.


book

APOE p.Leu167del-Related Lipid Disorders
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Greyshock,N., Guyton,J.R., Sebastian,S. and Okorodudu,D.


book

Alzheimer Disease Overview
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Bird,T.D.


book

Human apolipoprotein E mRNA. cDNA cloning and nucleotide sequencing of a new variant
J. Biol. Chem. 259 (10), 6498-6504 (1984)
McLean,J.W., Elshourbagy,N.A., Chang,D.J., Mahley,R.W. and Taylor,J.M.


book

Polymorphism of apolipoprotein E. III. Effect of a single polymorphic gene locus on plasma lipid levels in man
Clin. Genet. 15 (1), 63-72 (1979)
Utermann,G., Pruin,N. and Steinmetz,A.


book

Apoprotein (E--A-II) complex of human plasma lipoproteins. II. Receptor binding activity of a high density lipoprotein subfraction modulated by the apo(E--A-II) complex
J. Biol. Chem. 253 (17), 6289-6295 (1978)
Innerarity,T.L., Mahley,R.W., Weisgraber,K.H. and Bersot,T.P.


book

Apoprotein (E--A-II) complex of human plasma lipoproteins. I. Characterization of this mixed disulfide and its identification in a high density lipoprotein subfraction
J. Biol. Chem. 253 (17), 6281-6288 (1978)
Weisgraber,K.H. and Mahley,R.W.


 
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