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APRT cDNA ORF clone, Homo sapiens (human)

Gene Symbol APRT
Entrez Gene ID 353
Full Name adenine phosphoribosyltransferase
Synonyms AMP, APRTD
General protein information
Preferred Names
adenine phosphoribosyltransferase
Names
adenine phosphoribosyltransferase
AMP diphosphorylase
AMP pyrophosphorylase
transphosphoribosidase
NP_000476.1
NP_001025189.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

16

16q24

Summary Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

102600

Disorder Html: Urolithiasis, 2,8-dihydroxyadenine (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000485 NP_000476 adenine phosphoribosyltransferase isoform a
NM_001030018 NP_001025189 adenine phosphoribosyltransferase isoform b



Homo sapiens (human) APRT NP_000476.1
Pan troglodytes (chimpanzee) APRT XP_003952966.1
Macaca mulatta (Rhesus monkey) APRT XP_001089867.1
Canis lupus familiaris (dog) APRT XP_536752.2
Bos taurus (cattle) APRT NP_001020505.1
Mus musculus (house mouse) Aprt NP_033828.2
Rattus norvegicus (Norway rat) Aprt NP_001013079.1
Gallus gallus (chicken) APRT NP_001264576.1
Danio rerio (zebrafish) aprt NP_956962.1
Drosophila melanogaster (fruit fly) Aprt NP_476637.1
Caenorhabditis elegans T19B4.3 NP_491663.1
Arabidopsis thaliana (thale cress) APT1 NP_564284.1
Xenopus (Silurana) tropicalis (western clawed frog) aprt NP_001007941.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following APRT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APRT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19571
NM_000485 Homo sapiens adenine phosphoribosyltransferase (APRT), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu16247
NM_001030018 Homo sapiens adenine phosphoribosyltransferase (APRT), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19571
Clone ID Related Accession (Same CDS sequence) NM_000485
Accession Version NM_000485.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 543bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product adenine phosphoribosyltransferase isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM423481.1 and BU507629.1. This sequence is a reference standard in the RefSeqGene project. On Aug 3, 2005 this sequence version replaced gi:4502170. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM562053.1, BI762211.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGGCCGACT CCGAGCTGCA GCTGGTTGAG CAGCGGATCC GCAGCTTCCC CGACTTCCCC 
ACCCCAGGCG TGGTATTCAG GGACATCTCG CCCGTCCTGA AGGACCCCGC CTCCTTCCGC
GCCGCCATCG GCCTCCTGGC GCGACACCTG AAGGCGACCC ACGGGGGCCG CATCGACTAC
ATCGCAGGCC TAGACTCCCG AGGCTTCCTC TTTGGCCCCT CCCTGGCCCA GGAGCTTGGA
CTGGGCTGCG TGCTCATCCG AAAGCGGGGG AAGCTGCCAG GCCCCACTCT GTGGGCCTCC
TATTCCCTGG AGTACGGGAA GGCTGAGCTG GAGATTCAGA AAGACGCCCT GGAGCCAGGA
CAGAGGGTGG TCGTCGTGGA TGATCTGCTG GCCACTGGTG GAACCATGAA CGCTGCCTGT
GAGCTGCTGG GCCGCCTGCA GGCTGAGGTC CTGGAGTGCG TGAGCCTGGT GGAGCTGACC
TCGCTTAAGG GCAGGGAGAA GCTGGCACCT GTACCCTTCT TCTCTCTCCT GCAGTATGAG
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000476.1
CDS36..578
Misc Feature(1)39..41(+)
Misc Feature(2)45..47(+)
Misc Feature(3)78..80(+)
Misc Feature(4)159..566(+)
Misc Feature(5)213..215(+)
Misc Feature(6)228..512(+)
Misc Feature(7)231..233(+)
Misc Feature(8)375..377(+)
Misc Feature(9)438..440(+)
Exon (1)1..115
Gene:APRT
Gene Synonym:
Exon (2)116..222
Gene:APRT
Gene Synonym:
Exon (3)223..356
Gene:APRT
Gene Synonym:
Exon (4)357..435
Gene:APRT
Gene Synonym:
Exon (5)436..807
Gene:APRT
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000485
Organism Homo sapiens (human)
Definition Homo sapiens adenine phosphoribosyltransferase (APRT), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000485

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCCGACT CCGAGCTGCA GCTGGTTGAG CAGCGGATCC GCAGCTTCCC CGACTTCCCC 
ACCCCAGGCG TGGTATTCAG GGACATCTCG CCCGTCCTGA AGGACCCCGC CTCCTTCCGC
GCCGCCATCG GCCTCCTGGC GCGACACCTG AAGGCGACCC ACGGGGGCCG CATCGACTAC
ATCGCAGGCC TAGACTCCCG AGGCTTCCTC TTTGGCCCCT CCCTGGCCCA GGAGCTTGGA
CTGGGCTGCG TGCTCATCCG AAAGCGGGGG AAGCTGCCAG GCCCCACTCT GTGGGCCTCC
TATTCCCTGG AGTACGGGAA GGCTGAGCTG GAGATTCAGA AAGACGCCCT GGAGCCAGGA
CAGAGGGTGG TCGTCGTGGA TGATCTGCTG GCCACTGGTG GAACCATGAA CGCTGCCTGT
GAGCTGCTGG GCCGCCTGCA GGCTGAGGTC CTGGAGTGCG TGAGCCTGGT GGAGCTGACC
TCGCTTAAGG GCAGGGAGAA GCTGGCACCT GTACCCTTCT TCTCTCTCCT GCAGTATGAG
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16247
Clone ID Related Accession (Same CDS sequence) NM_001030018
Accession Version NM_001030018.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 405bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product adenine phosphoribosyltransferase isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM423481.1 and BM550173.1. Transcript Variant: This variant (2) lacks an alternate segment compared to variant 1, that causes a frameshift. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM550173.1, BI834522.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
ATGGCCGACT CCGAGCTGCA GCTGGTTGAG CAGCGGATCC GCAGCTTCCC CGACTTCCCC 
ACCCCAGGCG TGGTATTCAG GGACATCTCG CCCGTCCTGA AGGACCCCGC CTCCTTCCGC
GCCGCCATCG GCCTCCTGGC GCGACACCTG AAGGCGACCC ACGGGGGCCG CATCGACTAC
ATCGCAGGCC TAGACTCCCG AGGCTTCCTC TTTGGCCCCT CCCTGGCCCA GGAGCTTGGA
CTGGGCTGCG TGCTCATCCG AAAGCGGGGG AAGCTGCCAG GCCCCACTCT GTGGGCCTCC
TATTCCCTGG AGTACGGGAA GGCTGAGCTG GAGATTCAGA AAGACGCCCT GGAGCCAGGA
CAGAGGGTGG TCGTCGTGGA TGATCTGCTG GCCACTGGTG TATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001025189.1
CDS36..440
Misc Feature(1)39..41(+)
Misc Feature(2)45..47(+)
Misc Feature(3)78..80(+)
Misc Feature(4)159..>434(+)
Misc Feature(5)213..215(+)
Misc Feature(6)228..434(+)
Misc Feature(7)231..233(+)
Misc Feature(8)375..377(+)
Exon (1)1..115
Gene:APRT
Gene Synonym:
Exon (2)116..222
Gene:APRT
Gene Synonym:
Exon (3)223..356
Gene:APRT
Gene Synonym:
Exon (4)357..435
Gene:APRT
Gene Synonym:
Exon (5)436..673
Gene:APRT
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001030018
Organism Homo sapiens (human)
Definition Homo sapiens adenine phosphoribosyltransferase (APRT), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001030018

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGGCCGACT CCGAGCTGCA GCTGGTTGAG CAGCGGATCC GCAGCTTCCC CGACTTCCCC 
ACCCCAGGCG TGGTATTCAG GGACATCTCG CCCGTCCTGA AGGACCCCGC CTCCTTCCGC
GCCGCCATCG GCCTCCTGGC GCGACACCTG AAGGCGACCC ACGGGGGCCG CATCGACTAC
ATCGCAGGCC TAGACTCCCG AGGCTTCCTC TTTGGCCCCT CCCTGGCCCA GGAGCTTGGA
CTGGGCTGCG TGCTCATCCG AAAGCGGGGG AAGCTGCCAG GCCCCACTCT GTGGGCCTCC
TATTCCCTGG AGTACGGGAA GGCTGAGCTG GAGATTCAGA AAGACGCCCT GGAGCCAGGA
CAGAGGGTGG TCGTCGTGGA TGATCTGCTG GCCACTGGTG TATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A Japanese boy with adenine phosphoribosyltransferase (APRT) deficiency caused by compound heterozygosity including a novel missense mutation in APRT gene
Acta Paediatr. 100 (12), E285-E288 (2011)
Nozue H, Kamoda T, Saitoh H, Ichikawa K and Taniguchi A.


book

The phosphorylation status of membrane-bound nucleoside diphosphate kinase in epithelia and the role of AMP
Mol. Cell. Biochem. 329 (1-2), 107-114 (2009)
Treharne KJ, Best OG and Mehta A.


book

Large-scale proteomics and phosphoproteomics of urinary exosomes
J. Am. Soc. Nephrol. 20 (2), 363-379 (2009)
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS and Knepper MA.


book

Structural complexes of human adenine phosphoribosyltransferase reveal novel features of the APRT catalytic mechanism
J. Biomol. Struct. Dyn. 25 (6), 589-597 (2008)
Silva CH, Silva M, Iulek J and Thiemann OH.


book

Clinical, biochemical and molecular diagnosis of a compound homozygote for the 254 bp deletion-8 bp insertion of the APRT gene suffering from severe renal failure
Clin. Biochem. 40 (1-2), 73-80 (2007)
Di Pietro V, Perruzza I, Amorini AM, Balducci A, Ceccarelli L, Lazzarino G, Barsotti P, Giardina B and Tavazzi B.


book

Adenine Phosphoribosyltransferase Deficiency
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Edvardsson,V.O., Palsson,R. and Sahota,A.


book

Only three mutations account for almost all defective alleles causing adenine phosphoribosyltransferase deficiency in Japanese patients
J. Clin. Invest. 90 (1), 130-135 (1992)
Kamatani N, Hakoda M, Otsuka S, Yoshikawa H and Kashiwazaki S.


book

Enzymes of the purine interconversion system in chronic lymphatic leukemia: decreased purine nucleoside phosphorylase and adenosine deaminase activity
Blut 39 (5), 309-315 (1979)
Ludwig,H., Kuzmits,R., Pietschmann,H. and Muller,M.M.


book

Human adenine phosphoribosyltransferase. Affinity purification, subunit structure, amino acid composition, and peptide mapping
J. Biol. Chem. 254 (15), 6951-6955 (1979)
Holden,J.A., Meredith,G.S. and Kelley,W.N.


book

Adenine phosphoribosyltransferase: a simple spectrophotometric assay and the incidence of mutation in the normal population
Biochem. Genet. 15 (3-4), 265-272 (1977)
Johnson,L.A., Gordon,R.B. and Emmerson,B.T.


 
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