Email to GenScript

Human  Mouse  Rat  Other  

Search Help

Human  Mouse  Rat  Other  

  • THAT   AND
  • THAT   AND


APRT adenine phosphoribosyltransferase [Homo sapiens (human)]

Gene Symbol APRT
Entrez Gene ID 353
Full Name adenine phosphoribosyltransferase
Synonyms AMP, APRTD
General protein information
Preferred Names
adenine phosphoribosyltransferase
Names
adenine phosphoribosyltransferase
AMP diphosphorylase
AMP pyrophosphorylase
transphosphoribosidase
NP_000476.1
NP_001025189.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

16

16q24

Summary Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

102600

Disorder Html: Urolithiasis, 2,8-dihydroxyadenine (3)
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu19571 NM_000485 Homo sapiens adenine phosphoribosyltransferase (APRT), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu16247 NM_001030018 Homo sapiens adenine phosphoribosyltransferase (APRT), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu19571D
Sequence Information ORF Nucleotide Sequence (Length: 543bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product adenine phosphoribosyltransferase isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM423481.1 and BU507629.1. This sequence is a reference standard in the RefSeqGene project. On Aug 3, 2005 this sequence version replaced gi:4502170. Summary: Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM562053.1, BI762211.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGGCCGACT CCGAGCTGCA GCTGGTTGAG CAGCGGATCC GCAGCTTCCC CGACTTCCCC 
ACCCCAGGCG TGGTATTCAG GGACATCTCG CCCGTCCTGA AGGACCCCGC CTCCTTCCGC
GCCGCCATCG GCCTCCTGGC GCGACACCTG AAGGCGACCC ACGGGGGCCG CATCGACTAC
ATCGCAGGCC TAGACTCCCG AGGCTTCCTC TTTGGCCCCT CCCTGGCCCA GGAGCTTGGA
CTGGGCTGCG TGCTCATCCG AAAGCGGGGG AAGCTGCCAG GCCCCACTCT GTGGGCCTCC
TATTCCCTGG AGTACGGGAA GGCTGAGCTG GAGATTCAGA AAGACGCCCT GGAGCCAGGA
CAGAGGGTGG TCGTCGTGGA TGATCTGCTG GCCACTGGTG GAACCATGAA CGCTGCCTGT
GAGCTGCTGG GCCGCCTGCA GGCTGAGGTC CTGGAGTGCG TGAGCCTGGT GGAGCTGACC
TCGCTTAAGG GCAGGGAGAA GCTGGCACCT GTACCCTTCT TCTCTCTCCT GCAGTATGAG
TGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_000476.1
CDS36..578
Misc Feature(1)39..41(+)
Misc Feature(2)45..47(+)
Misc Feature(3)78..80(+)
Misc Feature(4)159..566(+)
Misc Feature(5)213..215(+)
Misc Feature(6)228..512(+)
Misc Feature(7)231..233(+)
Misc Feature(8)375..377(+)
Misc Feature(9)438..440(+)
Exon (1)1..115
Gene:APRT
Gene Synonym:
Exon (2)116..222
Gene:APRT
Gene Synonym:
Exon (3)223..356
Gene:APRT
Gene Synonym:
Exon (4)357..435
Gene:APRT
Gene Synonym:
Exon (5)436..807
Gene:APRT
Gene Synonym:
Translation
Position Chain Variation Link
1 1 a, g dbSNP:554162848
3 3 c, g dbSNP:765021715
6 6 c, g dbSNP:759534438
13 13 c, t dbSNP:572112498
14 14 c, t dbSNP:766477503
17 17 a, t dbSNP:2911456
19 19 a, g dbSNP:773547053
24 24 c, t dbSNP:772446991
26 26 g, t dbSNP:748455830
28 28 a, c, g, t dbSNP:371641686
32 32 g, t dbSNP:780463451
34 34 c, t dbSNP:368525048
39 39 g, t dbSNP:756735798
41 41 a, c dbSNP:746541257
42 42 a, g dbSNP:777362132
43 43 a, g dbSNP:758054373
44 44 c, t dbSNP:752381175
46 46 c, g dbSNP:765071048
47 47 c, t dbSNP:754800082
54 54 c, t dbSNP:556666445
80 80 c, t dbSNP:766475947
82 82 a, t dbSNP:760789463
86 86 -, c dbSNP:767912828
90 90 c, t dbSNP:773428808
91 91 a, t dbSNP:767789453
98 98 a, c dbSNP:762079076
107 107 g, t dbSNP:774501510
118 118 a, t dbSNP:764240833
120 120 a, t dbSNP:763334808
123 123 c, t dbSNP:775833509
125 125 g, t dbSNP:8191472
127 127 c, t dbSNP:149476467
128 128 c, t dbSNP:777192813
132 132 c, t dbSNP:8191473
137 137 a, g dbSNP:747661920
141 141 c, t dbSNP:778579890
148 148 c, g dbSNP:768226148
151 151 a, t dbSNP:749052990
153 153 c, t dbSNP:779980225
159 159 g, t dbSNP:756040710
160 160 -, c dbSNP:35148077
161 161 c, t dbSNP:750354445
166 166 g, t dbSNP:540432661
170 170 c, t dbSNP:200598967
178 178 g, t dbSNP:751620107
182 182 c, g dbSNP:764307769
191 191 c, g dbSNP:763101649
195 195 c, g dbSNP:752977102
197 197 c, t dbSNP:145490332
198 198 c, g dbSNP:759938204
201 201 a, g dbSNP:777100954
204 204 a, c dbSNP:766817564
205 205 g, t dbSNP:370646722
210 210 a, c, g dbSNP:768268700
213 213 g, t dbSNP:369570262
216 216 a, g dbSNP:748942141
218 218 a, c dbSNP:376027558
220 220 c, t dbSNP:201579274
229 229 a, t dbSNP:104894506
234 234 c, t dbSNP:369681854
235 235 a, g dbSNP:762509151
251 251 a, c dbSNP:377050219
253 253 a, c dbSNP:764922604
254 254 c, t dbSNP:140329016
255 255 c, t dbSNP:557418989
257 257 c, t dbSNP:538606935
258 258 c, g dbSNP:552995359
264 264 c, t dbSNP:760572304
269 269 c, g dbSNP:772978779
270 270 c, t dbSNP:772029639
272 272 c, t dbSNP:571299240
276 276 c, g dbSNP:778979771
281 281 a, c dbSNP:546635982
284 284 c, g dbSNP:749439155
285 285 a, g dbSNP:200392753
287 287 a, g dbSNP:756384230
294 294 a, c, t dbSNP:3169258
295 295 a, g dbSNP:117335001
296 296 -, ccga dbSNP:281860265
299 299 a, g dbSNP:138781159
300 300 c, t dbSNP:752166198
301 301 a, g dbSNP:150156607
305 305 -, g dbSNP:761430319
305 305 a, g, t dbSNP:200417820
305 305 -, g dbSNP:764924480
309 309 c, t dbSNP:753415188
311 311 a, g dbSNP:372253865
314 314 a, t dbSNP:780413670
315 315 -, ggcccca dbSNP:776240467
321 321 a, g dbSNP:530663323
323 323 c, t dbSNP:563575862
328 328 a, g dbSNP:281860264
329 329 a, g dbSNP:104894507
332 332 c, t dbSNP:281860262
336 336 c, t dbSNP:551418842
341 341 c, t dbSNP:768699313
350 350 c, t dbSNP:202098006
351 351 a, g dbSNP:780098835
353 353 a, g dbSNP:770055962
356 356 a, g dbSNP:368062984
360 360 a, g, t dbSNP:368375344
363 363 c, g, t dbSNP:533009428
364 364 c, t dbSNP:104894508
365 365 a, g dbSNP:750053169
369 369 a, t dbSNP:767177754
372 372 c, g, t dbSNP:376111313
376 376 a, c dbSNP:151240811
377 377 -, a dbSNP:760635677
380 380 c, t dbSNP:763956969
381 381 a, g dbSNP:201944035
382 382 c, t dbSNP:775353984
387 387 c, g dbSNP:370665100
389 389 a, g dbSNP:528515938
394 394 a, g dbSNP:776948275
397 397 a, g dbSNP:8191494
398 398 a, g dbSNP:747315064
399 399 a, c dbSNP:35095508
400 400 a, g dbSNP:772379415
401 401 a, g, t dbSNP:542789654
402 402 c, g dbSNP:755599871
404 404 a, g dbSNP:201227087
405 405 a, g dbSNP:780755086
407 407 c, t dbSNP:756911786
408 408 a, g dbSNP:376629164
410 410 a, c, t dbSNP:763991694
411 411 a, g dbSNP:75205792
420 420 c, t dbSNP:376129456
423 423 -, ctggccactg dbSNP:775204282
432 432 a, g dbSNP:759652584
441 441 a, g dbSNP:772611737
442 442 c, t dbSNP:28999113
446 446 c, t dbSNP:142472789
447 447 a, g dbSNP:747755586
458 458 a, g dbSNP:774096932
466 466 a, g dbSNP:569734625
466 466 -, g dbSNP:756672917
468 468 a, c, t dbSNP:550667813
469 469 a, g dbSNP:147688262
474 474 c, t dbSNP:745872435
476 476 a, g dbSNP:781123799
479 479 c, t dbSNP:145607240
483 483 g, t dbSNP:281860266
488 488 a, g dbSNP:369507525
492 492 g, t dbSNP:764321075
493 493 a, g dbSNP:758724286
494 494 c, t dbSNP:375131814
495 495 a, g dbSNP:141669365
506 506 a, g dbSNP:367605558
507 507 a, g dbSNP:777265858
517 517 c, t dbSNP:766909450
518 518 a, g dbSNP:761438940
522 522 a, g dbSNP:774100663
526 526 a, g dbSNP:768425517
536 536 c, g dbSNP:373775974
542 542 a, g dbSNP:775409590
546 546 a, g dbSNP:769693316
547 547 c, t dbSNP:745761689
549 549 c, t dbSNP:781239221
552 552 -, ttc dbSNP:121912681
554 554 c, g, t dbSNP:140070863
556 556 -, tct dbSNP:751152783
556 556 g, t dbSNP:370980256
561 561 -, ctcct dbSNP:755380873
562 562 -, tc dbSNP:781486778
563 563 c, t dbSNP:777907885
564 564 c, t dbSNP:565636938
569 569 a, g dbSNP:547319605
570 570 -, tatg dbSNP:751820457
576 576 c, t dbSNP:758634272
577 577 c, g dbSNP:387906584
579 579 c, t dbSNP:752975128
580 580 c, t dbSNP:375180338
581 581 a, g dbSNP:2070256
589 589 c, t dbSNP:567519373
592 592 c, t dbSNP:374275550
593 593 a, c dbSNP:761192074
599 599 a, g dbSNP:751125608
601 601 a, g dbSNP:763752678
608 608 a, g dbSNP:773463923
609 609 c, t dbSNP:775317972
610 610 c, t dbSNP:769454426
617 617 -, ca dbSNP:765012459
617 617 c, t dbSNP:549382452
622 622 a, c dbSNP:776390101
624 624 a, g dbSNP:528396845
625 625 c, t dbSNP:8191497
627 627 c, t dbSNP:777817751
661 661 a, g dbSNP:748634790
662 662 c, g dbSNP:766484349
666 666 c, t dbSNP:781776802
694 694 c, g dbSNP:772207278
699 699 c, g dbSNP:748342342
700 700 c, t dbSNP:769271336
701 701 a, g dbSNP:369966180
709 709 g, t dbSNP:754262300
713 713 c, g dbSNP:780365387
714 714 -, c dbSNP:756939078
722 722 g, t dbSNP:756627246
730 730 c, t dbSNP:137965502
732 732 c, g dbSNP:181364821
733 733 a, g dbSNP:560072301
735 735 c, g dbSNP:752347890
755 755 c, t dbSNP:764972083
756 756 a, c, g dbSNP:4695
757 757 c, t dbSNP:561477057
760 760 a, g dbSNP:8191498
766 766 a, g dbSNP:773174285
775 775 a, g dbSNP:772121530
777 777 c, t dbSNP:748252442
778 778 a, g dbSNP:758560583
795 795 a, g dbSNP:768930726
798 798 c, t dbSNP:749529041
804 804 a, c dbSNP:3177509

Target ORF information:

RefSeq Version NM_000485
Organism Homo sapiens (human)
Definition Homo sapiens adenine phosphoribosyltransferase (APRT), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_000485

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCCGACT CCGAGCTGCA GCTGGTTGAG CAGCGGATCC GCAGCTTCCC CGACTTCCCC 
ACCCCAGGCG TGGTATTCAG GGACATCTCG CCCGTCCTGA AGGACCCCGC CTCCTTCCGC
GCCGCCATCG GCCTCCTGGC GCGACACCTG AAGGCGACCC ACGGGGGCCG CATCGACTAC
ATCGCAGGCC TAGACTCCCG AGGCTTCCTC TTTGGCCCCT CCCTGGCCCA GGAGCTTGGA
CTGGGCTGCG TGCTCATCCG AAAGCGGGGG AAGCTGCCAG GCCCCACTCT GTGGGCCTCC
TATTCCCTGG AGTACGGGAA GGCTGAGCTG GAGATTCAGA AAGACGCCCT GGAGCCAGGA
CAGAGGGTGG TCGTCGTGGA TGATCTGCTG GCCACTGGTG GAACCATGAA CGCTGCCTGT
GAGCTGCTGG GCCGCCTGCA GGCTGAGGTC CTGGAGTGCG TGAGCCTGGT GGAGCTGACC
TCGCTTAAGG GCAGGGAGAA GCTGGCACCT GTACCCTTCT TCTCTCTCCT GCAGTATGAG
TGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu16247D
Sequence Information ORF Nucleotide Sequence (Length: 405bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product adenine phosphoribosyltransferase isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM423481.1 and BM550173.1. Summary: Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (2) lacks an alternate segment compared to variant 1, that causes a frameshift. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM550173.1, BI834522.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
ATGGCCGACT CCGAGCTGCA GCTGGTTGAG CAGCGGATCC GCAGCTTCCC CGACTTCCCC 
ACCCCAGGCG TGGTATTCAG GGACATCTCG CCCGTCCTGA AGGACCCCGC CTCCTTCCGC
GCCGCCATCG GCCTCCTGGC GCGACACCTG AAGGCGACCC ACGGGGGCCG CATCGACTAC
ATCGCAGGCC TAGACTCCCG AGGCTTCCTC TTTGGCCCCT CCCTGGCCCA GGAGCTTGGA
CTGGGCTGCG TGCTCATCCG AAAGCGGGGG AAGCTGCCAG GCCCCACTCT GTGGGCCTCC
TATTCCCTGG AGTACGGGAA GGCTGAGCTG GAGATTCAGA AAGACGCCCT GGAGCCAGGA
CAGAGGGTGG TCGTCGTGGA TGATCTGCTG GCCACTGGTG TATGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001025189.1
CDS36..440
Misc Feature(1)39..41(+)
Misc Feature(2)45..47(+)
Misc Feature(3)78..80(+)
Misc Feature(4)159..>434(+)
Misc Feature(5)213..215(+)
Misc Feature(6)228..434(+)
Misc Feature(7)231..233(+)
Misc Feature(8)375..377(+)
Exon (1)1..115
Gene:APRT
Gene Synonym:
Exon (2)116..222
Gene:APRT
Gene Synonym:
Exon (3)223..356
Gene:APRT
Gene Synonym:
Exon (4)357..435
Gene:APRT
Gene Synonym:
Exon (5)436..673
Gene:APRT
Gene Synonym:
Translation
Position Chain Variation Link
1 1 a, g dbSNP:554162848
3 3 c, g dbSNP:765021715
6 6 c, g dbSNP:759534438
13 13 c, t dbSNP:572112498
14 14 c, t dbSNP:766477503
17 17 a, t dbSNP:2911456
19 19 a, g dbSNP:773547053
24 24 c, t dbSNP:772446991
26 26 g, t dbSNP:748455830
28 28 a, c, g, t dbSNP:371641686
32 32 g, t dbSNP:780463451
34 34 c, t dbSNP:368525048
39 39 g, t dbSNP:756735798
41 41 a, c dbSNP:746541257
42 42 a, g dbSNP:777362132
43 43 a, g dbSNP:758054373
44 44 c, t dbSNP:752381175
46 46 c, g dbSNP:765071048
47 47 c, t dbSNP:754800082
54 54 c, t dbSNP:556666445
80 80 c, t dbSNP:766475947
82 82 a, t dbSNP:760789463
86 86 -, c dbSNP:767912828
90 90 c, t dbSNP:773428808
91 91 a, t dbSNP:767789453
98 98 a, c dbSNP:762079076
107 107 g, t dbSNP:774501510
118 118 a, t dbSNP:764240833
120 120 a, t dbSNP:763334808
123 123 c, t dbSNP:775833509
125 125 g, t dbSNP:8191472
127 127 c, t dbSNP:149476467
128 128 c, t dbSNP:777192813
132 132 c, t dbSNP:8191473
137 137 a, g dbSNP:747661920
141 141 c, t dbSNP:778579890
148 148 c, g dbSNP:768226148
151 151 a, t dbSNP:749052990
153 153 c, t dbSNP:779980225
159 159 g, t dbSNP:756040710
160 160 -, c dbSNP:35148077
161 161 c, t dbSNP:750354445
166 166 g, t dbSNP:540432661
170 170 c, t dbSNP:200598967
178 178 g, t dbSNP:751620107
182 182 c, g dbSNP:764307769
191 191 c, g dbSNP:763101649
195 195 c, g dbSNP:752977102
197 197 c, t dbSNP:145490332
198 198 c, g dbSNP:759938204
201 201 a, g dbSNP:777100954
204 204 a, c dbSNP:766817564
205 205 g, t dbSNP:370646722
210 210 a, c, g dbSNP:768268700
213 213 g, t dbSNP:369570262
216 216 a, g dbSNP:748942141
218 218 a, c dbSNP:376027558
220 220 c, t dbSNP:201579274
229 229 a, t dbSNP:104894506
234 234 c, t dbSNP:369681854
235 235 a, g dbSNP:762509151
251 251 a, c dbSNP:377050219
253 253 a, c dbSNP:764922604
254 254 c, t dbSNP:140329016
255 255 c, t dbSNP:557418989
257 257 c, t dbSNP:538606935
258 258 c, g dbSNP:552995359
264 264 c, t dbSNP:760572304
269 269 c, g dbSNP:772978779
270 270 c, t dbSNP:772029639
272 272 c, t dbSNP:571299240
276 276 c, g dbSNP:778979771
281 281 a, c dbSNP:546635982
284 284 c, g dbSNP:749439155
285 285 a, g dbSNP:200392753
287 287 a, g dbSNP:756384230
294 294 a, c, t dbSNP:3169258
295 295 a, g dbSNP:117335001
296 296 -, ccga dbSNP:281860265
299 299 a, g dbSNP:138781159
300 300 c, t dbSNP:752166198
301 301 a, g dbSNP:150156607
305 305 -, g dbSNP:761430319
305 305 a, g, t dbSNP:200417820
305 305 -, g dbSNP:764924480
309 309 c, t dbSNP:753415188
311 311 a, g dbSNP:372253865
314 314 a, t dbSNP:780413670
315 315 -, ggcccca dbSNP:776240467
321 321 a, g dbSNP:530663323
323 323 c, t dbSNP:563575862
328 328 a, g dbSNP:281860264
329 329 a, g dbSNP:104894507
332 332 c, t dbSNP:281860262
336 336 c, t dbSNP:551418842
341 341 c, t dbSNP:768699313
350 350 c, t dbSNP:202098006
351 351 a, g dbSNP:780098835
353 353 a, g dbSNP:770055962
356 356 a, g dbSNP:368062984
360 360 a, g, t dbSNP:368375344
363 363 c, g, t dbSNP:533009428
364 364 c, t dbSNP:104894508
365 365 a, g dbSNP:750053169
369 369 a, t dbSNP:767177754
372 372 c, g, t dbSNP:376111313
376 376 a, c dbSNP:151240811
377 377 -, a dbSNP:760635677
380 380 c, t dbSNP:763956969
381 381 a, g dbSNP:201944035
382 382 c, t dbSNP:775353984
387 387 c, g dbSNP:370665100
389 389 a, g dbSNP:528515938
394 394 a, g dbSNP:776948275
397 397 a, g dbSNP:8191494
398 398 a, g dbSNP:747315064
399 399 a, c dbSNP:35095508
400 400 a, g dbSNP:772379415
401 401 a, g, t dbSNP:542789654
402 402 c, g dbSNP:755599871
404 404 a, g dbSNP:201227087
405 405 a, g dbSNP:780755086
407 407 c, t dbSNP:756911786
408 408 a, g dbSNP:376629164
410 410 a, c, t dbSNP:763991694
411 411 a, g dbSNP:75205792
420 420 c, t dbSNP:376129456
423 423 -, ctggccactg dbSNP:775204282
432 432 a, g dbSNP:759652584
436 436 -, tatg dbSNP:751820457
442 442 c, t dbSNP:758634272
443 443 c, g dbSNP:387906584
445 445 c, t dbSNP:752975128
446 446 c, t dbSNP:375180338
447 447 a, g dbSNP:2070256
455 455 c, t dbSNP:567519373
458 458 c, t dbSNP:374275550
459 459 a, c dbSNP:761192074
465 465 a, g dbSNP:751125608
467 467 a, g dbSNP:763752678
474 474 a, g dbSNP:773463923
475 475 c, t dbSNP:775317972
476 476 c, t dbSNP:769454426
483 483 -, ca dbSNP:765012459
483 483 c, t dbSNP:549382452
488 488 a, c dbSNP:776390101
490 490 a, g dbSNP:528396845
491 491 c, t dbSNP:8191497
493 493 c, t dbSNP:777817751
527 527 a, g dbSNP:748634790
528 528 c, g dbSNP:766484349
532 532 c, t dbSNP:781776802
560 560 c, g dbSNP:772207278
565 565 c, g dbSNP:748342342
566 566 c, t dbSNP:769271336
567 567 a, g dbSNP:369966180
575 575 g, t dbSNP:754262300
579 579 c, g dbSNP:780365387
580 580 -, c dbSNP:756939078
588 588 g, t dbSNP:756627246
596 596 c, t dbSNP:137965502
598 598 c, g dbSNP:181364821
599 599 a, g dbSNP:560072301
601 601 c, g dbSNP:752347890
621 621 c, t dbSNP:764972083
622 622 a, c, g dbSNP:4695
623 623 c, t dbSNP:561477057
626 626 a, g dbSNP:8191498
632 632 a, g dbSNP:773174285
641 641 a, g dbSNP:772121530
643 643 c, t dbSNP:748252442
644 644 a, g dbSNP:758560583
661 661 a, g dbSNP:768930726
664 664 c, t dbSNP:749529041
670 670 a, c dbSNP:3177509

Target ORF information:

RefSeq Version NM_001030018
Organism Homo sapiens (human)
Definition Homo sapiens adenine phosphoribosyltransferase (APRT), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001030018

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGGCCGACT CCGAGCTGCA GCTGGTTGAG CAGCGGATCC GCAGCTTCCC CGACTTCCCC 
ACCCCAGGCG TGGTATTCAG GGACATCTCG CCCGTCCTGA AGGACCCCGC CTCCTTCCGC
GCCGCCATCG GCCTCCTGGC GCGACACCTG AAGGCGACCC ACGGGGGCCG CATCGACTAC
ATCGCAGGCC TAGACTCCCG AGGCTTCCTC TTTGGCCCCT CCCTGGCCCA GGAGCTTGGA
CTGGGCTGCG TGCTCATCCG AAAGCGGGGG AAGCTGCCAG GCCCCACTCT GTGGGCCTCC
TATTCCCTGG AGTACGGGAA GGCTGAGCTG GAGATTCAGA AAGACGCCCT GGAGCCAGGA
CAGAGGGTGG TCGTCGTGGA TGATCTGCTG GCCACTGGTG TATGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.