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KLK3 kallikrein-related peptidase 3 [Homo sapiens (human)]

Gene Symbol KLK3
Entrez Gene ID 354
Full Name kallikrein-related peptidase 3
Synonyms APS, KLK2A1, PSA, hK3
General protein information
Preferred Names
prostate-specific antigen
Names
prostate-specific antigen
seminin
P-30 antigen
kallikrein-3
semenogelase
gamma-seminoprotein
prostate specific antigen
NP_001025218.1
NP_001025219.1
NP_001025221.1
NP_001639.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19q13.41

Summary Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

176820

Disorder Html:





The following KLK3 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KLK3 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu56336 XM_011526923 PREDICTED: Homo sapiens kallikrein-related peptidase 3 (KLK3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu56337 XM_011526924 PREDICTED: Homo sapiens kallikrein-related peptidase 3 (KLK3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu23388 NM_001648 Homo sapiens kallikrein-related peptidase 3 (KLK3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99
OHu23435 NM_001030047 Homo sapiens kallikrein-related peptidase 3 (KLK3), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$269
OHu23926 NM_001030048 Homo sapiens kallikrein-related peptidase 3 (KLK3), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu56336
Accession Version XM_011526923.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 804bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product prostate-specific antigen isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011109.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTGGGTCC CGGTTGTCTT CCTCACCCTG TCCGTGACGT GGATTGGTGC TGCACCCCTC 
ATCCTGTCTC GGATTGTGGG AGGCTGGGAG TGCGAGAAGC ATTCCCAACC CTGGCAGGTG
CTTGTGGCCT CTCGTGGCAG GGCAGTCTGC GGCGGTGTTC TGGTGCACCC CCAGTGGGTC
CTCACAGCTG CCCACTGCAT CAGGAACAAA AGCGTGATCT TGCTGGGTCG GCACAGCCTG
TTTCATCCTG AAGACACAGG CCAGGTATTT CAGGTCAGCC ACAGCTTCCC ACACCCGCTC
TACGATATGA GCCTCCTGAA GAATCGATTC CTCAGGCCAG GTGATGACTC CAGCCACGAC
CTCATGCTGC TCCGCCTGTC AGAGCCTGCC GAGCTCACGG ATGCTGTGAA GGTCATGGAC
CTGCCCACCC AGGAGCCAGC ACTGGGGACC ACCTGCTACG CCTCAGGCTG GGGCAGCATT
GAACCAGAGG AGTGTACGCC TGGGCCAGAT GTCTTGACCC CAAAGAAACT TCAGTGTGTG
GACCTCCATG TTATTTCCAA TGACGTGTGT GCGCAAGTTC ACCCTCAGAA GGTGACCAAG
TTCATGCTGT GTGCTGGACG CTGGACAGGG GGCAAAAGCA CCTGCTCGGG TGATTCTGGG
GGCCCACTTG TCTGTAATGG TGTGCTTCAA GGTATCACGT CATGGGGCAG TGAACCATGT
GCCCTGCCCG AAAGGCCTTC CCTGTACACC AAGGTGGTGC ATTACCGGAA GTGGATCAAG
GACACCATCG TGGCCAACCC CTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011525225.1
CDS36..839
Misc Feature(1)105..812(+)
Misc Feature(2)108..821(+)
Misc Feature(3)108..110(+)
Misc Feature(4)228..692(+)
Misc Feature(5)672..743(+)
Translation
Position Chain Variation Link
1 1 a, c dbSNP:780950315
11 11 c, t dbSNP:370025467
15 15 c, t dbSNP:769236849
19 19 c, t dbSNP:199680464
20 20 a, g dbSNP:762592843
23 23 a, g dbSNP:751938250
34 34 c, t dbSNP:373854842
40 40 -, g dbSNP:779162374
44 44 c, t dbSNP:201712212
46 46 c, t dbSNP:111689449
47 47 a, g dbSNP:375104394
61 61 a, c dbSNP:368127664
67 67 a, c, t dbSNP:138400533
68 68 c, t dbSNP:765400712
69 69 a, g dbSNP:750712372
71 71 c, g dbSNP:758622865
73 73 c, t dbSNP:780090483
74 74 a, g dbSNP:751400607
81 81 g, t dbSNP:754663333
83 83 c, t dbSNP:17849961
83 83 c, t dbSNP:11573
85 85 c, t dbSNP:181375335
88 88 c, t dbSNP:750316227
89 89 a, g dbSNP:1135766
95 95 a, c dbSNP:765982632
105 105 c, t dbSNP:141576693
106 106 a, g dbSNP:147446084
109 109 c, t dbSNP:375110405
110 110 c, t dbSNP:754188902
112 112 a, t dbSNP:757579274
116 116 a, c dbSNP:200846924
119 119 c, t dbSNP:746365590
124 124 a, g, t dbSNP:772176502
125 125 a, g dbSNP:547221078
128 128 c, t dbSNP:139911579
129 129 a, g dbSNP:2271092
141 141 c, t dbSNP:747908647
144 144 c, t dbSNP:769617725
145 145 a, c dbSNP:773350836
148 148 g, t dbSNP:762952938
151 151 a, t dbSNP:200712655
152 152 a, g dbSNP:7252245
155 155 g, t dbSNP:568880974
157 157 c, t dbSNP:767083316
163 163 a, c dbSNP:140318583
164 164 c, g, t dbSNP:374094231
168 168 c, t dbSNP:555200638
169 169 a, g dbSNP:139560851
171 171 -, gg dbSNP:775070174
175 175 a, g dbSNP:758925761
177 177 a, g dbSNP:149709822
180 180 c, g dbSNP:747094712
181 181 -, a dbSNP:34953540
185 185 c, t dbSNP:144294681
186 186 c, g dbSNP:781327047
188 188 -, ggg dbSNP:375428882
188 188 c, t dbSNP:201710884
189 189 a, g dbSNP:369223448
198 198 a, g dbSNP:773127519
201 201 c, t dbSNP:577778784
203 203 c, g dbSNP:770994770
205 205 c, t dbSNP:545098001
206 206 c, g dbSNP:758987002
207 207 a, c, t dbSNP:767027476
210 210 g, t dbSNP:760366035
215 215 c, t dbSNP:763682700
218 218 c, t dbSNP:750794289
231 231 c, t dbSNP:371595805
248 248 c, g, t dbSNP:756104515
249 249 a, g dbSNP:148892721
257 257 a, c, g dbSNP:150517791
264 264 c, t dbSNP:146422657
265 265 a, c, g dbSNP:140831892
266 266 g, t dbSNP:746553268
269 269 c, g dbSNP:768134516
272 272 c, t dbSNP:12946
273 273 c, t dbSNP:17849962
282 282 c, t dbSNP:761450479
284 284 c, t dbSNP:771342720
294 294 a, c, g dbSNP:138565183
295 295 a, g dbSNP:768098832
299 299 c, g dbSNP:753094271
302 302 a, g dbSNP:760883577
331 331 c, t dbSNP:764233923
332 332 a, g, t dbSNP:754175332
335 335 c, g dbSNP:2739452
338 338 c, t dbSNP:374344392
339 339 a, g dbSNP:61752561
340 340 a, t dbSNP:758082019
341 341 a, c, t dbSNP:780090257
344 344 a, g dbSNP:754550408
350 350 c, t dbSNP:780667166
358 358 a, g dbSNP:747600000
360 360 c, t dbSNP:769439514
361 361 a, g dbSNP:772699340
365 365 c, t dbSNP:746264869
368 368 c, t dbSNP:772299876
372 372 c, g dbSNP:776101817
375 375 g, t dbSNP:574935823
376 376 a, g dbSNP:764323663
381 381 a, g dbSNP:776860388
382 382 a, c dbSNP:761947130
384 384 c, t dbSNP:765521022
392 392 c, g, t dbSNP:377532899
393 393 a, g dbSNP:766084011
395 395 c, t dbSNP:542099924
396 396 c, t dbSNP:754921426
405 405 c, t dbSNP:201390347
408 408 c, t dbSNP:61750343
409 409 a, g dbSNP:755527546
420 420 a, c, t dbSNP:527812439
425 425 c, t dbSNP:201110725
426 426 a, g, t dbSNP:182759459
429 429 a, c, t dbSNP:2003783
433 433 c, t dbSNP:373661565
434 434 a, g dbSNP:765201777
439 439 c, t dbSNP:773552177
441 441 a, g, t dbSNP:149427770
443 443 a, g dbSNP:751359189
445 445 a, g dbSNP:754728036
451 451 g, t dbSNP:767463542
461 461 c, t dbSNP:752607029
482 482 a, g, t dbSNP:147547638
491 491 c, t dbSNP:753580440
494 494 c, t dbSNP:376483907
495 495 a, g dbSNP:138273452
497 497 -, t dbSNP:756135278
509 509 a, c, t dbSNP:747404815
511 511 a, g dbSNP:781775024
513 513 a, t dbSNP:200126385
515 515 g, t dbSNP:748662283
519 519 c, t dbSNP:112372888
524 524 a, g dbSNP:143868213
527 527 c, g dbSNP:763023030
528 528 g, t dbSNP:771089837
530 530 c, t dbSNP:548130809
532 532 c, t dbSNP:375786338
533 533 a, c, g dbSNP:566539425
535 535 c, g, t dbSNP:752696686
540 540 c, t dbSNP:764030989
541 541 c, t dbSNP:753323492
547 547 c, t dbSNP:778191056
548 548 a, c, t dbSNP:749770722
553 553 c, t dbSNP:367976209
556 556 c, t dbSNP:745885851
565 565 a, t dbSNP:772282891
571 571 a, g dbSNP:775735844
578 578 c, t dbSNP:202206082
581 581 c, g dbSNP:148735869
582 582 c, t dbSNP:776501538
584 584 c, t dbSNP:372190695
589 589 c, t dbSNP:17632542
598 598 a, t dbSNP:772853400
599 599 a, c, t dbSNP:372148450
600 600 a, g dbSNP:150769385
607 607 c, t dbSNP:554107787
608 608 a, g dbSNP:376510386
616 616 a, g dbSNP:759706621
620 620 a, t dbSNP:572619039
627 627 a, g, t dbSNP:376934756
636 636 a, t dbSNP:745974856
641 641 c, g dbSNP:139172057
644 644 c, g dbSNP:758455175
646 646 g, t dbSNP:780184499
653 653 a, c, g dbSNP:1803129
654 654 c, t dbSNP:546294568
656 656 c, t dbSNP:370658603
658 658 g, t dbSNP:187449592
659 659 g, t dbSNP:191588971
661 661 a, c, t dbSNP:146528783
667 667 a, g dbSNP:762600618
682 682 c, g, t dbSNP:61729813
683 683 a, g dbSNP:759209785
686 686 a, t dbSNP:764929426
687 687 a, g dbSNP:151298874
692 692 -, t dbSNP:766453393
694 694 a, g dbSNP:750895817
697 697 g, t dbSNP:750179092
698 698 c, g dbSNP:549637095
699 699 a, c dbSNP:779926782
709 709 g, t dbSNP:746545348
713 713 c, t dbSNP:754464009
716 716 c, t dbSNP:140564011
733 733 c, t dbSNP:138882542
734 734 a, g, t dbSNP:61736309
742 742 a, g dbSNP:200116262
746 746 c, t dbSNP:772734530
752 752 a, g dbSNP:79245399
762 762 a, c dbSNP:144118163
764 764 c, t dbSNP:146297845
765 765 a, g dbSNP:372901531
772 772 c, t dbSNP:776973438
773 773 c, t dbSNP:45552633
779 779 a, g dbSNP:113126792
789 789 g, t dbSNP:538660698
792 792 g, t dbSNP:762688864
798 798 c, t dbSNP:1802720
800 800 c, t dbSNP:779407068
801 801 c, t dbSNP:746367915
802 802 a, g dbSNP:149376489
810 810 a, g, t dbSNP:548476435
820 820 c, t dbSNP:144748315
822 822 a, g dbSNP:752193560
824 824 c, t dbSNP:375475599
825 825 a, g, t dbSNP:200006942
826 826 a, t dbSNP:772540456
834 834 a, c, t dbSNP:780743219
836 836 c, t dbSNP:769230318
839 839 a, c dbSNP:776581784
841 841 c, t dbSNP:536820435
849 849 -, t dbSNP:753859707
853 853 c, t dbSNP:761917326
854 854 c, t dbSNP:1058205
861 861 c, t dbSNP:773462440
862 862 g, t dbSNP:762778769
863 863 c, t dbSNP:766160591
864 864 a, g dbSNP:373134993
866 866 c, t dbSNP:759482291
869 869 a, g dbSNP:374859717
878 878 c, t dbSNP:368035787
879 879 c, t dbSNP:752179012
889 889 c, g dbSNP:112479005
898 898 c, g dbSNP:141498977
904 904 a, g dbSNP:1058274
906 906 a, c dbSNP:749897325
916 916 a, t dbSNP:1803132
928 928 c, t dbSNP:1802722
930 930 c, t dbSNP:1141894
937 937 a, g dbSNP:577670828
956 956 c, t dbSNP:116064912
963 963 a, g dbSNP:1802721
968 968 c, t dbSNP:563949982
975 975 a, g dbSNP:116248649
976 976 a, c dbSNP:3180906
988 988 a, c dbSNP:569814469
999 999 c, g dbSNP:561507023
1001 1001 c, t dbSNP:16987771
1027 1027 c, t dbSNP:1803138
1039 1039 c, t dbSNP:572690444
1053 1053 a, g dbSNP:766084172
1080 1080 c, g dbSNP:190504941
1084 1084 c, g, t dbSNP:12101
1095 1095 c, g dbSNP:774491454
1108 1108 a, g dbSNP:546140331
1113 1113 c, t dbSNP:538641946
1117 1117 a, g dbSNP:6998
1119 1119 a, g dbSNP:569833730
1142 1142 g, t dbSNP:537315899
1145 1145 a, c dbSNP:16987775
1171 1171 c, t dbSNP:752217318
1217 1217 c, t dbSNP:555394775
1221 1221 c, t dbSNP:1803136
1226 1226 c, g dbSNP:573733138
1232 1232 c, t dbSNP:534527207
1239 1239 a, g dbSNP:1803135
1247 1247 g, t dbSNP:370827372
1252 1252 a, g dbSNP:193123776
1254 1254 a, g dbSNP:377210991
1259 1259 c, t dbSNP:1803134
1261 1261 a, c dbSNP:538849894
1283 1283 a, g dbSNP:12040
1299 1299 a, g dbSNP:61269009
1303 1303 g, t dbSNP:754156080
1309 1309 a, g dbSNP:556963317
1336 1336 c, t dbSNP:1803133
1343 1343 a, g dbSNP:11554029
1346 1346 a, g dbSNP:779131129
1354 1354 a, g dbSNP:12863
1371 1371 a, g dbSNP:750975726
1373 1373 a, g dbSNP:1803137
1378 1378 a, g dbSNP:11554028
1387 1387 -, g dbSNP:780652548
1387 1387 g, t dbSNP:575154555
1388 1388 c, g dbSNP:543260524
1428 1428 c, t dbSNP:11554027
1447 1447 c, t dbSNP:561435501
1472 1472 c, t dbSNP:758871345

Target ORF information:

RefSeq Version XM_011526923
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens kallikrein-related peptidase 3 (KLK3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011526923

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTGGGTCC CGGTTGTCTT CCTCACCCTG TCCGTGACGT GGATTGGTGC TGCACCCCTC 
ATCCTGTCTC GGATTGTGGG AGGCTGGGAG TGCGAGAAGC ATTCCCAACC CTGGCAGGTG
CTTGTGGCCT CTCGTGGCAG GGCAGTCTGC GGCGGTGTTC TGGTGCACCC CCAGTGGGTC
CTCACAGCTG CCCACTGCAT CAGGAACAAA AGCGTGATCT TGCTGGGTCG GCACAGCCTG
TTTCATCCTG AAGACACAGG CCAGGTATTT CAGGTCAGCC ACAGCTTCCC ACACCCGCTC
TACGATATGA GCCTCCTGAA GAATCGATTC CTCAGGCCAG GTGATGACTC CAGCCACGAC
CTCATGCTGC TCCGCCTGTC AGAGCCTGCC GAGCTCACGG ATGCTGTGAA GGTCATGGAC
CTGCCCACCC AGGAGCCAGC ACTGGGGACC ACCTGCTACG CCTCAGGCTG GGGCAGCATT
GAACCAGAGG AGTGTACGCC TGGGCCAGAT GTCTTGACCC CAAAGAAACT TCAGTGTGTG
GACCTCCATG TTATTTCCAA TGACGTGTGT GCGCAAGTTC ACCCTCAGAA GGTGACCAAG
TTCATGCTGT GTGCTGGACG CTGGACAGGG GGCAAAAGCA CCTGCTCGGG TGATTCTGGG
GGCCCACTTG TCTGTAATGG TGTGCTTCAA GGTATCACGT CATGGGGCAG TGAACCATGT
GCCCTGCCCG AAAGGCCTTC CCTGTACACC AAGGTGGTGC ATTACCGGAA GTGGATCAAG
GACACCATCG TGGCCAACCC CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56337
Accession Version XM_011526924.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 735bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product prostate-specific antigen isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011109.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTGGGTCC CGGTTGTCTT CCTCACCCTG TCCGTGACGT GGATTGGTGC TGCACCCCTC 
ATCCTGTCTC GGATTGTGGG AGGCTGGGAG TGCGAGAAGC ATTCCCAACC CTGGCAGGTG
CTTGTGGCCT CTCGTGGCAG GGCAGTCTGC GGCGGTGTTC TGGTGCACCC CCAGTGGGTC
CTCACAGCTG CCCACTGCAT CAGGAACAAA AGCGTGATCT TGCTGGGTCG GCACAGCCTG
TTTCATCCTG AAGACACAGG CCAGGTATTT CAGGTCAGCC ACAGCTTCCC ACACCCGCTC
TACGATATGA GCCTCCTGAA GAATCGATTC CTCAGGCCAG GTGATGACTC CAGCCACGAC
CTCATGCTGC TCCGCCTGTC AGAGCCTGCC GAGCTCACGG ATGCTGTGAA GGTCATGGAC
CTGCCCACCC AGGAGCCAGC ACTGGGGACC ACCTGCTACG CCTCAGGCTG GGGCAGCATT
GAACCAGAGG AGTGTACGCC TGGGCCAGAT GTCTTGACCC CAAAGAAACT TCAGTGTGTG
GACCTCCATG TTATTTCCAA TGACGTGTGT GCGCAAGTTC ACCCTCAGAA GGTGACCAAG
TTCATGCTGT GTGCTGGACG CTGGACAGGG GGCAAAAGCA CCTGCTCGTG GGTCATTCTG
ATCACCGAAC TGACCATGCC AGCCCTGCCG ATGGTCCTCC ATGGCTCCCT AGTGCCCTGG
AGAGGAGGTG TCTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011525226.1
CDS36..770
Misc Feature(1)105..>680(+)
Misc Feature(2)108..>680(+)
Misc Feature(3)108..110(+)
Misc Feature(4)228..395(+)
Translation
Position Chain Variation Link
1 1 a, c dbSNP:780950315
11 11 c, t dbSNP:370025467
15 15 c, t dbSNP:769236849
19 19 c, t dbSNP:199680464
20 20 a, g dbSNP:762592843
23 23 a, g dbSNP:751938250
34 34 c, t dbSNP:373854842
40 40 -, g dbSNP:779162374
44 44 c, t dbSNP:201712212
46 46 c, t dbSNP:111689449
47 47 a, g dbSNP:375104394
61 61 a, c dbSNP:368127664
67 67 a, c, t dbSNP:138400533
68 68 c, t dbSNP:765400712
69 69 a, g dbSNP:750712372
71 71 c, g dbSNP:758622865
73 73 c, t dbSNP:780090483
74 74 a, g dbSNP:751400607
81 81 g, t dbSNP:754663333
83 83 c, t dbSNP:17849961
83 83 c, t dbSNP:11573
85 85 c, t dbSNP:181375335
88 88 c, t dbSNP:750316227
89 89 a, g dbSNP:1135766
95 95 a, c dbSNP:765982632
105 105 c, t dbSNP:141576693
106 106 a, g dbSNP:147446084
109 109 c, t dbSNP:375110405
110 110 c, t dbSNP:754188902
112 112 a, t dbSNP:757579274
116 116 a, c dbSNP:200846924
119 119 c, t dbSNP:746365590
124 124 a, g, t dbSNP:772176502
125 125 a, g dbSNP:547221078
128 128 c, t dbSNP:139911579
129 129 a, g dbSNP:2271092
141 141 c, t dbSNP:747908647
144 144 c, t dbSNP:769617725
145 145 a, c dbSNP:773350836
148 148 g, t dbSNP:762952938
151 151 a, t dbSNP:200712655
152 152 a, g dbSNP:7252245
155 155 g, t dbSNP:568880974
157 157 c, t dbSNP:767083316
163 163 a, c dbSNP:140318583
164 164 c, g, t dbSNP:374094231
168 168 c, t dbSNP:555200638
169 169 a, g dbSNP:139560851
171 171 -, gg dbSNP:775070174
175 175 a, g dbSNP:758925761
177 177 a, g dbSNP:149709822
180 180 c, g dbSNP:747094712
181 181 -, a dbSNP:34953540
185 185 c, t dbSNP:144294681
186 186 c, g dbSNP:781327047
188 188 -, ggg dbSNP:375428882
188 188 c, t dbSNP:201710884
189 189 a, g dbSNP:369223448
198 198 a, g dbSNP:773127519
201 201 c, t dbSNP:577778784
203 203 c, g dbSNP:770994770
205 205 c, t dbSNP:545098001
206 206 c, g dbSNP:758987002
207 207 a, c, t dbSNP:767027476
210 210 g, t dbSNP:760366035
215 215 c, t dbSNP:763682700
218 218 c, t dbSNP:750794289
231 231 c, t dbSNP:371595805
248 248 c, g, t dbSNP:756104515
249 249 a, g dbSNP:148892721
257 257 a, c, g dbSNP:150517791
264 264 c, t dbSNP:146422657
265 265 a, c, g dbSNP:140831892
266 266 g, t dbSNP:746553268
269 269 c, g dbSNP:768134516
272 272 c, t dbSNP:12946
273 273 c, t dbSNP:17849962
282 282 c, t dbSNP:761450479
284 284 c, t dbSNP:771342720
294 294 a, c, g dbSNP:138565183
295 295 a, g dbSNP:768098832
299 299 c, g dbSNP:753094271
302 302 a, g dbSNP:760883577
331 331 c, t dbSNP:764233923
332 332 a, g, t dbSNP:754175332
335 335 c, g dbSNP:2739452
338 338 c, t dbSNP:374344392
339 339 a, g dbSNP:61752561
340 340 a, t dbSNP:758082019
341 341 a, c, t dbSNP:780090257
344 344 a, g dbSNP:754550408
350 350 c, t dbSNP:780667166
358 358 a, g dbSNP:747600000
360 360 c, t dbSNP:769439514
361 361 a, g dbSNP:772699340
365 365 c, t dbSNP:746264869
368 368 c, t dbSNP:772299876
372 372 c, g dbSNP:776101817
375 375 g, t dbSNP:574935823
376 376 a, g dbSNP:764323663
381 381 a, g dbSNP:776860388
382 382 a, c dbSNP:761947130
384 384 c, t dbSNP:765521022
392 392 c, g, t dbSNP:377532899
393 393 a, g dbSNP:766084011
395 395 c, t dbSNP:542099924
396 396 c, t dbSNP:754921426
405 405 c, t dbSNP:201390347
408 408 c, t dbSNP:61750343
409 409 a, g dbSNP:755527546
420 420 a, c, t dbSNP:527812439
425 425 c, t dbSNP:201110725
426 426 a, g, t dbSNP:182759459
429 429 a, c, t dbSNP:2003783
433 433 c, t dbSNP:373661565
434 434 a, g dbSNP:765201777
439 439 c, t dbSNP:773552177
441 441 a, g, t dbSNP:149427770
443 443 a, g dbSNP:751359189
445 445 a, g dbSNP:754728036
451 451 g, t dbSNP:767463542
461 461 c, t dbSNP:752607029
482 482 a, g, t dbSNP:147547638
491 491 c, t dbSNP:753580440
494 494 c, t dbSNP:376483907
495 495 a, g dbSNP:138273452
497 497 -, t dbSNP:756135278
509 509 a, c, t dbSNP:747404815
511 511 a, g dbSNP:781775024
513 513 a, t dbSNP:200126385
515 515 g, t dbSNP:748662283
519 519 c, t dbSNP:112372888
524 524 a, g dbSNP:143868213
527 527 c, g dbSNP:763023030
528 528 g, t dbSNP:771089837
530 530 c, t dbSNP:548130809
532 532 c, t dbSNP:375786338
533 533 a, c, g dbSNP:566539425
535 535 c, g, t dbSNP:752696686
540 540 c, t dbSNP:764030989
541 541 c, t dbSNP:753323492
547 547 c, t dbSNP:778191056
548 548 a, c, t dbSNP:749770722
553 553 c, t dbSNP:367976209
556 556 c, t dbSNP:745885851
565 565 a, t dbSNP:772282891
571 571 a, g dbSNP:775735844
578 578 c, t dbSNP:202206082
581 581 c, g dbSNP:148735869
582 582 c, t dbSNP:776501538
584 584 c, t dbSNP:372190695
589 589 c, t dbSNP:17632542
598 598 a, t dbSNP:772853400
599 599 a, c, t dbSNP:372148450
600 600 a, g dbSNP:150769385
607 607 c, t dbSNP:554107787
608 608 a, g dbSNP:376510386
616 616 a, g dbSNP:759706621
620 620 a, t dbSNP:572619039
627 627 a, g, t dbSNP:376934756
636 636 a, t dbSNP:745974856
641 641 c, g dbSNP:139172057
644 644 c, g dbSNP:758455175
646 646 g, t dbSNP:780184499
653 653 a, c, g dbSNP:1803129
654 654 c, t dbSNP:546294568
656 656 c, t dbSNP:370658603
658 658 g, t dbSNP:187449592
659 659 g, t dbSNP:191588971
661 661 a, c, t dbSNP:146528783
667 667 a, g dbSNP:762600618
682 682 c, g, t dbSNP:61729813
683 683 a, g dbSNP:759209785
690 690 a, t dbSNP:749029980
697 697 a, t dbSNP:770902213
701 701 c, t dbSNP:35192866
702 702 a, g dbSNP:73932617
707 707 a, g dbSNP:148839360
711 711 a, g dbSNP:537643737
714 714 c, t dbSNP:760312184
722 722 g, t dbSNP:768243223
723 723 a, c dbSNP:529790605
724 724 c, t dbSNP:199786442
725 725 a, g dbSNP:371037518
726 726 a, g dbSNP:766933442
728 728 a, g dbSNP:774699638
736 736 a, g dbSNP:759700116
737 737 c, t dbSNP:766283610
743 743 c, t dbSNP:767699197
744 744 c, t dbSNP:143518180
750 750 c, t dbSNP:756417848
763 763 a, g dbSNP:534688911
770 770 c, g dbSNP:753713626
772 772 c, t dbSNP:756951776
777 777 a, t dbSNP:778955963
784 784 c, t dbSNP:745792138
795 795 c, t dbSNP:757986467
798 798 c, t dbSNP:10401479
805 805 a, g dbSNP:201907046
809 809 c, t dbSNP:746453452
811 811 c, t dbSNP:368022457
812 812 a, g dbSNP:768329109
812 812 -, g dbSNP:756290566
813 813 a, g dbSNP:776216918
819 819 a, g dbSNP:200639337
839 839 a, g dbSNP:10401978
852 852 c, t dbSNP:575794794
853 853 a, g dbSNP:45588133
864 864 a, t dbSNP:561622740
924 924 c, t dbSNP:2659122
949 949 c, t dbSNP:764141650
955 955 a, g dbSNP:752451895
963 963 c, t dbSNP:574525519
988 988 a, g dbSNP:540652721
1010 1010 g, t dbSNP:778027460
1014 1014 c, t dbSNP:3209092
1029 1029 a, g dbSNP:559356703
1045 1045 c, t dbSNP:532978563
1082 1082 a, g dbSNP:771167591
1085 1085 a, g dbSNP:749612200
1095 1095 g, t dbSNP:529947489
1101 1101 a, c dbSNP:759946194
1102 1102 a, c dbSNP:768078108
1104 1104 c, t dbSNP:775712442
1108 1108 c, t dbSNP:760793589
1109 1109 a, c, t dbSNP:764431782
1112 1112 g, t dbSNP:757145681
1128 1128 a, t dbSNP:764929426
1129 1129 a, g dbSNP:151298874
1134 1134 -, t dbSNP:766453393
1136 1136 a, g dbSNP:750895817
1139 1139 g, t dbSNP:750179092
1140 1140 c, g dbSNP:549637095
1141 1141 a, c dbSNP:779926782
1151 1151 g, t dbSNP:746545348
1155 1155 c, t dbSNP:754464009
1158 1158 c, t dbSNP:140564011

Target ORF information:

RefSeq Version XM_011526924
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens kallikrein-related peptidase 3 (KLK3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011526924

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTGGGTCC CGGTTGTCTT CCTCACCCTG TCCGTGACGT GGATTGGTGC TGCACCCCTC 
ATCCTGTCTC GGATTGTGGG AGGCTGGGAG TGCGAGAAGC ATTCCCAACC CTGGCAGGTG
CTTGTGGCCT CTCGTGGCAG GGCAGTCTGC GGCGGTGTTC TGGTGCACCC CCAGTGGGTC
CTCACAGCTG CCCACTGCAT CAGGAACAAA AGCGTGATCT TGCTGGGTCG GCACAGCCTG
TTTCATCCTG AAGACACAGG CCAGGTATTT CAGGTCAGCC ACAGCTTCCC ACACCCGCTC
TACGATATGA GCCTCCTGAA GAATCGATTC CTCAGGCCAG GTGATGACTC CAGCCACGAC
CTCATGCTGC TCCGCCTGTC AGAGCCTGCC GAGCTCACGG ATGCTGTGAA GGTCATGGAC
CTGCCCACCC AGGAGCCAGC ACTGGGGACC ACCTGCTACG CCTCAGGCTG GGGCAGCATT
GAACCAGAGG AGTGTACGCC TGGGCCAGAT GTCTTGACCC CAAAGAAACT TCAGTGTGTG
GACCTCCATG TTATTTCCAA TGACGTGTGT GCGCAAGTTC ACCCTCAGAA GGTGACCAAG
TTCATGCTGT GTGCTGGACG CTGGACAGGG GGCAAAAGCA CCTGCTCGTG GGTCATTCTG
ATCACCGAAC TGACCATGCC AGCCCTGCCG ATGGTCCTCC ATGGCTCCCT AGTGCCCTGG
AGAGGAGGTG TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23388
Accession Version NM_001648.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 786bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu23388D_COA.pdf (pdf)
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product prostate-specific antigen isoform 1 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X05332.1. This sequence is a reference standard in the RefSeqGene project. On Aug 12, 2002 this sequence version replaced gi:4502172. Summary: Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (1) encodes the longest isoform (1) of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X05332.1, M26663.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTGGGTCC CGGTTGTCTT CCTCACCCTG TCCGTGACGT GGATTGGTGC TGCACCCCTC 
ATCCTGTCTC GGATTGTGGG AGGCTGGGAG TGCGAGAAGC ATTCCCAACC CTGGCAGGTG
CTTGTGGCCT CTCGTGGCAG GGCAGTCTGC GGCGGTGTTC TGGTGCACCC CCAGTGGGTC
CTCACAGCTG CCCACTGCAT CAGGAACAAA AGCGTGATCT TGCTGGGTCG GCACAGCCTG
TTTCATCCTG AAGACACAGG CCAGGTATTT CAGGTCAGCC ACAGCTTCCC ACACCCGCTC
TACGATATGA GCCTCCTGAA GAATCGATTC CTCAGGCCAG GTGATGACTC CAGCCACGAC
CTCATGCTGC TCCGCCTGTC AGAGCCTGCC GAGCTCACGG ATGCTGTGAA GGTCATGGAC
CTGCCCACCC AGGAGCCAGC ACTGGGGACC ACCTGCTACG CCTCAGGCTG GGGCAGCATT
GAACCAGAGG AGTTCTTGAC CCCAAAGAAA CTTCAGTGTG TGGACCTCCA TGTTATTTCC
AATGACGTGT GTGCGCAAGT TCACCCTCAG AAGGTGACCA AGTTCATGCT GTGTGCTGGA
CGCTGGACAG GGGGCAAAAG CACCTGCTCG GGTGATTCTG GGGGCCCACT TGTCTGTAAT
GGTGTGCTTC AAGGTATCAC GTCATGGGGC AGTGAACCAT GTGCCCTGCC CGAAAGGCCT
TCCCTGTACA CCAAGGTGGT GCATTACCGG AAGTGGATCA AGGACACCAT CGTGGCCAAC
CCCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001639.1
CDS42..827
Misc Feature(1)111..800(+)
Misc Feature(2)114..809(+)
Misc Feature(3)114..116(+)
Misc Feature(4)234..680(+)
Misc Feature(5)246..248(+)
Misc Feature(6)273..275(+)
Misc Feature(7)282..284(+)
Misc Feature(8)546..548(+)
Misc Feature(9)660..731(+)
Exon (1)1..87
Gene:KLK3
Gene Synonym:
Exon (2)88..247
Gene:KLK3
Gene Synonym:
Exon (3)248..534
Gene:KLK3
Gene Synonym:
Exon (4)535..671
Gene:KLK3
Gene Synonym:
Exon (5)672..1464
Gene:KLK3
Gene Synonym:
Translation
Position Chain Variation Link
3 3 c, t dbSNP:371797932
7 7 a, c dbSNP:780950315
17 17 c, t dbSNP:370025467
21 21 c, t dbSNP:769236849
25 25 c, t dbSNP:199680464
26 26 a, g dbSNP:762592843
29 29 a, g dbSNP:751938250
40 40 c, t dbSNP:373854842
46 46 -, g dbSNP:779162374
50 50 c, t dbSNP:201712212
52 52 c, t dbSNP:111689449
53 53 a, g dbSNP:375104394
67 67 a, c dbSNP:368127664
73 73 a, c, t dbSNP:138400533
74 74 c, t dbSNP:765400712
75 75 a, g dbSNP:750712372
77 77 c, g dbSNP:758622865
79 79 c, t dbSNP:780090483
80 80 a, g dbSNP:751400607
87 87 g, t dbSNP:754663333
89 89 c, t dbSNP:17849961
89 89 c, t dbSNP:11573
91 91 c, t dbSNP:181375335
94 94 c, t dbSNP:750316227
95 95 a, g dbSNP:1135766
101 101 a, c dbSNP:765982632
111 111 c, t dbSNP:141576693
112 112 a, g dbSNP:147446084
115 115 c, t dbSNP:375110405
116 116 c, t dbSNP:754188902
118 118 a, t dbSNP:757579274
122 122 a, c dbSNP:200846924
125 125 c, t dbSNP:746365590
130 130 a, g, t dbSNP:772176502
131 131 a, g dbSNP:547221078
134 134 c, t dbSNP:139911579
135 135 a, g dbSNP:2271092
147 147 c, t dbSNP:747908647
150 150 c, t dbSNP:769617725
151 151 a, c dbSNP:773350836
154 154 g, t dbSNP:762952938
157 157 a, t dbSNP:200712655
158 158 a, g dbSNP:7252245
161 161 g, t dbSNP:568880974
163 163 c, t dbSNP:767083316
169 169 a, c dbSNP:140318583
170 170 c, g, t dbSNP:374094231
174 174 c, t dbSNP:555200638
175 175 a, g dbSNP:139560851
177 177 -, gg dbSNP:775070174
181 181 a, g dbSNP:758925761
183 183 a, g dbSNP:149709822
186 186 c, g dbSNP:747094712
187 187 -, a dbSNP:34953540
191 191 c, t dbSNP:144294681
192 192 c, g dbSNP:781327047
194 194 -, ggg dbSNP:375428882
194 194 c, t dbSNP:201710884
195 195 a, g dbSNP:369223448
204 204 a, g dbSNP:773127519
207 207 c, t dbSNP:577778784
209 209 c, g dbSNP:770994770
211 211 c, t dbSNP:545098001
212 212 c, g dbSNP:758987002
213 213 a, c, t dbSNP:767027476
216 216 g, t dbSNP:760366035
221 221 c, t dbSNP:763682700
224 224 c, t dbSNP:750794289
237 237 c, t dbSNP:371595805
254 254 c, g, t dbSNP:756104515
255 255 a, g dbSNP:148892721
263 263 a, c, g dbSNP:150517791
270 270 c, t dbSNP:146422657
271 271 a, c, g dbSNP:140831892
272 272 g, t dbSNP:746553268
275 275 c, g dbSNP:768134516
278 278 c, t dbSNP:12946
279 279 c, t dbSNP:17849962
288 288 c, t dbSNP:761450479
290 290 c, t dbSNP:771342720
300 300 a, c, g dbSNP:138565183
301 301 a, g dbSNP:768098832
305 305 c, g dbSNP:753094271
308 308 a, g dbSNP:760883577
337 337 c, t dbSNP:764233923
338 338 a, g, t dbSNP:754175332
341 341 c, g dbSNP:2739452
344 344 c, t dbSNP:374344392
345 345 a, g dbSNP:61752561
346 346 a, t dbSNP:758082019
347 347 a, c, t dbSNP:780090257
350 350 a, g dbSNP:754550408
356 356 c, t dbSNP:780667166
364 364 a, g dbSNP:747600000
366 366 c, t dbSNP:769439514
367 367 a, g dbSNP:772699340
371 371 c, t dbSNP:746264869
374 374 c, t dbSNP:772299876
378 378 c, g dbSNP:776101817
381 381 g, t dbSNP:574935823
382 382 a, g dbSNP:764323663
387 387 a, g dbSNP:776860388
388 388 a, c dbSNP:761947130
390 390 c, t dbSNP:765521022
398 398 c, g, t dbSNP:377532899
399 399 a, g dbSNP:766084011
401 401 c, t dbSNP:542099924
402 402 c, t dbSNP:754921426
411 411 c, t dbSNP:201390347
414 414 c, t dbSNP:61750343
415 415 a, g dbSNP:755527546
426 426 a, c, t dbSNP:527812439
431 431 c, t dbSNP:201110725
432 432 a, g, t dbSNP:182759459
435 435 a, c, t dbSNP:2003783
439 439 c, t dbSNP:373661565
440 440 a, g dbSNP:765201777
445 445 c, t dbSNP:773552177
447 447 a, g, t dbSNP:149427770
449 449 a, g dbSNP:751359189
451 451 a, g dbSNP:754728036
457 457 g, t dbSNP:767463542
467 467 c, t dbSNP:752607029
488 488 a, g, t dbSNP:147547638
497 497 c, t dbSNP:753580440
500 500 c, t dbSNP:376483907
501 501 a, g dbSNP:138273452
503 503 -, t dbSNP:756135278
515 515 a, c, t dbSNP:747404815
517 517 a, g dbSNP:781775024
519 519 a, t dbSNP:200126385
521 521 g, t dbSNP:748662283
525 525 c, t dbSNP:112372888
530 530 a, g dbSNP:143868213
533 533 c, g dbSNP:763023030
534 534 g, t dbSNP:771089837
535 535 c, t dbSNP:778191056
536 536 a, c, t dbSNP:749770722
541 541 c, t dbSNP:367976209
544 544 c, t dbSNP:745885851
553 553 a, t dbSNP:772282891
559 559 a, g dbSNP:775735844
566 566 c, t dbSNP:202206082
569 569 c, g dbSNP:148735869
570 570 c, t dbSNP:776501538
572 572 c, t dbSNP:372190695
577 577 c, t dbSNP:17632542
586 586 a, t dbSNP:772853400
587 587 a, c, t dbSNP:372148450
588 588 a, g dbSNP:150769385
595 595 c, t dbSNP:554107787
596 596 a, g dbSNP:376510386
604 604 a, g dbSNP:759706621
608 608 a, t dbSNP:572619039
615 615 a, g, t dbSNP:376934756
624 624 a, t dbSNP:745974856
629 629 c, g dbSNP:139172057
632 632 c, g dbSNP:758455175
634 634 g, t dbSNP:780184499
641 641 a, c, g dbSNP:1803129
642 642 c, t dbSNP:546294568
644 644 c, t dbSNP:370658603
646 646 g, t dbSNP:187449592
647 647 g, t dbSNP:191588971
649 649 a, c, t dbSNP:146528783
655 655 a, g dbSNP:762600618
670 670 c, g, t dbSNP:61729813
671 671 a, g dbSNP:759209785
674 674 a, t dbSNP:764929426
675 675 a, g dbSNP:151298874
680 680 -, t dbSNP:766453393
682 682 a, g dbSNP:750895817
685 685 g, t dbSNP:750179092
686 686 c, g dbSNP:549637095
687 687 a, c dbSNP:779926782
697 697 g, t dbSNP:746545348
701 701 c, t dbSNP:754464009
704 704 c, t dbSNP:140564011
721 721 c, t dbSNP:138882542
722 722 a, g, t dbSNP:61736309
730 730 a, g dbSNP:200116262
734 734 c, t dbSNP:772734530
740 740 a, g dbSNP:79245399
750 750 a, c dbSNP:144118163
752 752 c, t dbSNP:146297845
753 753 a, g dbSNP:372901531
760 760 c, t dbSNP:776973438
761 761 c, t dbSNP:45552633
767 767 a, g dbSNP:113126792
777 777 g, t dbSNP:538660698
780 780 g, t dbSNP:762688864
786 786 c, t dbSNP:1802720
788 788 c, t dbSNP:779407068
789 789 c, t dbSNP:746367915
790 790 a, g dbSNP:149376489
798 798 a, g, t dbSNP:548476435
808 808 c, t dbSNP:144748315
810 810 a, g dbSNP:752193560
812 812 c, t dbSNP:375475599
813 813 a, g, t dbSNP:200006942
814 814 a, t dbSNP:772540456
822 822 a, c, t dbSNP:780743219
824 824 c, t dbSNP:769230318
827 827 a, c dbSNP:776581784
829 829 c, t dbSNP:536820435
837 837 -, t dbSNP:753859707
841 841 c, t dbSNP:761917326
842 842 c, t dbSNP:1058205
849 849 c, t dbSNP:773462440
850 850 g, t dbSNP:762778769
851 851 c, t dbSNP:766160591
852 852 a, g dbSNP:373134993
854 854 c, t dbSNP:759482291
857 857 a, g dbSNP:374859717
866 866 c, t dbSNP:368035787
867 867 c, t dbSNP:752179012
877 877 c, g dbSNP:112479005
886 886 c, g dbSNP:141498977
892 892 a, g dbSNP:1058274
894 894 a, c dbSNP:749897325
904 904 a, t dbSNP:1803132
916 916 c, t dbSNP:1802722
918 918 c, t dbSNP:1141894
925 925 a, g dbSNP:577670828
944 944 c, t dbSNP:116064912
951 951 a, g dbSNP:1802721
956 956 c, t dbSNP:563949982
963 963 a, g dbSNP:116248649
964 964 a, c dbSNP:3180906
976 976 a, c dbSNP:569814469
987 987 c, g dbSNP:561507023
989 989 c, t dbSNP:16987771
1015 1015 c, t dbSNP:1803138
1027 1027 c, t dbSNP:572690444
1041 1041 a, g dbSNP:766084172
1068 1068 c, g dbSNP:190504941
1072 1072 c, g, t dbSNP:12101
1083 1083 c, g dbSNP:774491454
1096 1096 a, g dbSNP:546140331
1101 1101 c, t dbSNP:538641946
1105 1105 a, g dbSNP:6998
1107 1107 a, g dbSNP:569833730
1130 1130 g, t dbSNP:537315899
1133 1133 a, c dbSNP:16987775
1159 1159 c, t dbSNP:752217318
1205 1205 c, t dbSNP:555394775
1209 1209 c, t dbSNP:1803136
1214 1214 c, g dbSNP:573733138
1220 1220 c, t dbSNP:534527207
1227 1227 a, g dbSNP:1803135
1235 1235 g, t dbSNP:370827372
1240 1240 a, g dbSNP:193123776
1242 1242 a, g dbSNP:377210991
1247 1247 c, t dbSNP:1803134
1249 1249 a, c dbSNP:538849894
1271 1271 a, g dbSNP:12040
1287 1287 a, g dbSNP:61269009
1291 1291 g, t dbSNP:754156080
1297 1297 a, g dbSNP:556963317
1324 1324 c, t dbSNP:1803133
1331 1331 a, g dbSNP:11554029
1334 1334 a, g dbSNP:779131129
1342 1342 a, g dbSNP:12863
1359 1359 a, g dbSNP:750975726
1361 1361 a, g dbSNP:1803137
1366 1366 a, g dbSNP:11554028
1375 1375 -, g dbSNP:780652548
1375 1375 g, t dbSNP:575154555
1376 1376 c, g dbSNP:543260524
1416 1416 c, t dbSNP:11554027
1435 1435 c, t dbSNP:561435501
1460 1460 c, t dbSNP:758871345

Target ORF information:

RefSeq Version NM_001648
Organism Homo sapiens (human)
Definition Homo sapiens kallikrein-related peptidase 3 (KLK3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001648

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTGGGTCC CGGTTGTCTT CCTCACCCTG TCCGTGACGT GGATTGGTGC TGCACCCCTC 
ATCCTGTCTC GGATTGTGGG AGGCTGGGAG TGCGAGAAGC ATTCCCAACC CTGGCAGGTG
CTTGTGGCCT CTCGTGGCAG GGCAGTCTGC GGCGGTGTTC TGGTGCACCC CCAGTGGGTC
CTCACAGCTG CCCACTGCAT CAGGAACAAA AGCGTGATCT TGCTGGGTCG GCACAGCCTG
TTTCATCCTG AAGACACAGG CCAGGTATTT CAGGTCAGCC ACAGCTTCCC ACACCCGCTC
TACGATATGA GCCTCCTGAA GAATCGATTC CTCAGGCCAG GTGATGACTC CAGCCACGAC
CTCATGCTGC TCCGCCTGTC AGAGCCTGCC GAGCTCACGG ATGCTGTGAA GGTCATGGAC
CTGCCCACCC AGGAGCCAGC ACTGGGGACC ACCTGCTACG CCTCAGGCTG GGGCAGCATT
GAACCAGAGG AGTTCTTGAC CCCAAAGAAA CTTCAGTGTG TGGACCTCCA TGTTATTTCC
AATGACGTGT GTGCGCAAGT TCACCCTCAG AAGGTGACCA AGTTCATGCT GTGTGCTGGA
CGCTGGACAG GGGGCAAAAG CACCTGCTCG GGTGATTCTG GGGGCCCACT TGTCTGTAAT
GGTGTGCTTC AAGGTATCAC GTCATGGGGC AGTGAACCAT GTGCCCTGCC CGAAAGGCCT
TCCCTGTACA CCAAGGTGGT GCATTACCGG AAGTGGATCA AGGACACCAT CGTGGCCAAC
CCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23435
Accession Version NM_001030047.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 717bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu23435D_COA.pdf (pdf)
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product prostate-specific antigen isoform 3 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC005307.1, M21896.1 and AC011523.3. Summary: Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (3) uses an alternate splice site at the end of a coding exon, that causes a frameshift. The resulting isoform (3) is shorter and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU932434.1, M21896.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGTGGGTCC CGGTTGTCTT CCTCACCCTG TCCGTGACGT GGATTGGTGC TGCACCCCTC 
ATCCTGTCTC GGATTGTGGG AGGCTGGGAG TGCGAGAAGC ATTCCCAACC CTGGCAGGTG
CTTGTGGCCT CTCGTGGCAG GGCAGTCTGC GGCGGTGTTC TGGTGCACCC CCAGTGGGTC
CTCACAGCTG CCCACTGCAT CAGGAACAAA AGCGTGATCT TGCTGGGTCG GCACAGCCTG
TTTCATCCTG AAGACACAGG CCAGGTATTT CAGGTCAGCC ACAGCTTCCC ACACCCGCTC
TACGATATGA GCCTCCTGAA GAATCGATTC CTCAGGCCAG GTGATGACTC CAGCCACGAC
CTCATGCTGC TCCGCCTGTC AGAGCCTGCC GAGCTCACGG ATGCTGTGAA GGTCATGGAC
CTGCCCACCC AGGAGCCAGC ACTGGGGACC ACCTGCTACG CCTCAGGCTG GGGCAGCATT
GAACCAGAGG AGTTCTTGAC CCCAAAGAAA CTTCAGTGTG TGGACCTCCA TGTTATTTCC
AATGACGTGT GTGCGCAAGT TCACCCTCAG AAGGTGACCA AGTTCATGCT GTGTGCTGGA
CGCTGGACAG GGGGCAAAAG CACCTGCTCG TGGGTCATTC TGATCACCGA ACTGACCATG
CCAGCCCTGC CGATGGTCCT CCATGGCTCC CTAGTGCCCT GGAGAGGAGG TGTCTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001025218.1
CDS42..758
Misc Feature(1)111..>668(+)
Misc Feature(2)114..>668(+)
Misc Feature(3)114..116(+)
Misc Feature(4)234..401(+)
Exon (1)1..87
Gene:KLK3
Gene Synonym:
Exon (2)88..247
Gene:KLK3
Gene Synonym:
Exon (3)248..534
Gene:KLK3
Gene Synonym:
Exon (4)535..671
Gene:KLK3
Gene Synonym:
Exon (5)672..1906
Gene:KLK3
Gene Synonym:
Translation
Position Chain Variation Link
3 3 c, t dbSNP:371797932
7 7 a, c dbSNP:780950315
17 17 c, t dbSNP:370025467
21 21 c, t dbSNP:769236849
25 25 c, t dbSNP:199680464
26 26 a, g dbSNP:762592843
29 29 a, g dbSNP:751938250
40 40 c, t dbSNP:373854842
46 46 -, g dbSNP:779162374
50 50 c, t dbSNP:201712212
52 52 c, t dbSNP:111689449
53 53 a, g dbSNP:375104394
67 67 a, c dbSNP:368127664
73 73 a, c, t dbSNP:138400533
74 74 c, t dbSNP:765400712
75 75 a, g dbSNP:750712372
77 77 c, g dbSNP:758622865
79 79 c, t dbSNP:780090483
80 80 a, g dbSNP:751400607
87 87 g, t dbSNP:754663333
89 89 c, t dbSNP:17849961
89 89 c, t dbSNP:11573
91 91 c, t dbSNP:181375335
94 94 c, t dbSNP:750316227
95 95 a, g dbSNP:1135766
101 101 a, c dbSNP:765982632
111 111 c, t dbSNP:141576693
112 112 a, g dbSNP:147446084
115 115 c, t dbSNP:375110405
116 116 c, t dbSNP:754188902
118 118 a, t dbSNP:757579274
122 122 a, c dbSNP:200846924
125 125 c, t dbSNP:746365590
130 130 a, g, t dbSNP:772176502
131 131 a, g dbSNP:547221078
134 134 c, t dbSNP:139911579
135 135 a, g dbSNP:2271092
147 147 c, t dbSNP:747908647
150 150 c, t dbSNP:769617725
151 151 a, c dbSNP:773350836
154 154 g, t dbSNP:762952938
157 157 a, t dbSNP:200712655
158 158 a, g dbSNP:7252245
161 161 g, t dbSNP:568880974
163 163 c, t dbSNP:767083316
169 169 a, c dbSNP:140318583
170 170 c, g, t dbSNP:374094231
174 174 c, t dbSNP:555200638
175 175 a, g dbSNP:139560851
177 177 -, gg dbSNP:775070174
181 181 a, g dbSNP:758925761
183 183 a, g dbSNP:149709822
186 186 c, g dbSNP:747094712
187 187 -, a dbSNP:34953540
191 191 c, t dbSNP:144294681
192 192 c, g dbSNP:781327047
194 194 -, ggg dbSNP:375428882
194 194 c, t dbSNP:201710884
195 195 a, g dbSNP:369223448
204 204 a, g dbSNP:773127519
207 207 c, t dbSNP:577778784
209 209 c, g dbSNP:770994770
211 211 c, t dbSNP:545098001
212 212 c, g dbSNP:758987002
213 213 a, c, t dbSNP:767027476
216 216 g, t dbSNP:760366035
221 221 c, t dbSNP:763682700
224 224 c, t dbSNP:750794289
237 237 c, t dbSNP:371595805
254 254 c, g, t dbSNP:756104515
255 255 a, g dbSNP:148892721
263 263 a, c, g dbSNP:150517791
270 270 c, t dbSNP:146422657
271 271 a, c, g dbSNP:140831892
272 272 g, t dbSNP:746553268
275 275 c, g dbSNP:768134516
278 278 c, t dbSNP:12946
279 279 c, t dbSNP:17849962
288 288 c, t dbSNP:761450479
290 290 c, t dbSNP:771342720
300 300 a, c, g dbSNP:138565183
301 301 a, g dbSNP:768098832
305 305 c, g dbSNP:753094271
308 308 a, g dbSNP:760883577
337 337 c, t dbSNP:764233923
338 338 a, g, t dbSNP:754175332
341 341 c, g dbSNP:2739452
344 344 c, t dbSNP:374344392
345 345 a, g dbSNP:61752561
346 346 a, t dbSNP:758082019
347 347 a, c, t dbSNP:780090257
350 350 a, g dbSNP:754550408
356 356 c, t dbSNP:780667166
364 364 a, g dbSNP:747600000
366 366 c, t dbSNP:769439514
367 367 a, g dbSNP:772699340
371 371 c, t dbSNP:746264869
374 374 c, t dbSNP:772299876
378 378 c, g dbSNP:776101817
381 381 g, t dbSNP:574935823
382 382 a, g dbSNP:764323663
387 387 a, g dbSNP:776860388
388 388 a, c dbSNP:761947130
390 390 c, t dbSNP:765521022
398 398 c, g, t dbSNP:377532899
399 399 a, g dbSNP:766084011
401 401 c, t dbSNP:542099924
402 402 c, t dbSNP:754921426
411 411 c, t dbSNP:201390347
414 414 c, t dbSNP:61750343
415 415 a, g dbSNP:755527546
426 426 a, c, t dbSNP:527812439
431 431 c, t dbSNP:201110725
432 432 a, g, t dbSNP:182759459
435 435 a, c, t dbSNP:2003783
439 439 c, t dbSNP:373661565
440 440 a, g dbSNP:765201777
445 445 c, t dbSNP:773552177
447 447 a, g, t dbSNP:149427770
449 449 a, g dbSNP:751359189
451 451 a, g dbSNP:754728036
457 457 g, t dbSNP:767463542
467 467 c, t dbSNP:752607029
488 488 a, g, t dbSNP:147547638
497 497 c, t dbSNP:753580440
500 500 c, t dbSNP:376483907
501 501 a, g dbSNP:138273452
503 503 -, t dbSNP:756135278
515 515 a, c, t dbSNP:747404815
517 517 a, g dbSNP:781775024
519 519 a, t dbSNP:200126385
521 521 g, t dbSNP:748662283
525 525 c, t dbSNP:112372888
530 530 a, g dbSNP:143868213
533 533 c, g dbSNP:763023030
534 534 g, t dbSNP:771089837
535 535 c, t dbSNP:778191056
536 536 a, c, t dbSNP:749770722
541 541 c, t dbSNP:367976209
544 544 c, t dbSNP:745885851
553 553 a, t dbSNP:772282891
559 559 a, g dbSNP:775735844
566 566 c, t dbSNP:202206082
569 569 c, g dbSNP:148735869
570 570 c, t dbSNP:776501538
572 572 c, t dbSNP:372190695
577 577 c, t dbSNP:17632542
586 586 a, t dbSNP:772853400
587 587 a, c, t dbSNP:372148450
588 588 a, g dbSNP:150769385
595 595 c, t dbSNP:554107787
596 596 a, g dbSNP:376510386
604 604 a, g dbSNP:759706621
608 608 a, t dbSNP:572619039
615 615 a, g, t dbSNP:376934756
624 624 a, t dbSNP:745974856
629 629 c, g dbSNP:139172057
632 632 c, g dbSNP:758455175
634 634 g, t dbSNP:780184499
641 641 a, c, g dbSNP:1803129
642 642 c, t dbSNP:546294568
644 644 c, t dbSNP:370658603
646 646 g, t dbSNP:187449592
647 647 g, t dbSNP:191588971
649 649 a, c, t dbSNP:146528783
655 655 a, g dbSNP:762600618
670 670 c, g, t dbSNP:61729813
671 671 a, g dbSNP:759209785
678 678 a, t dbSNP:749029980
685 685 a, t dbSNP:770902213
689 689 c, t dbSNP:35192866
690 690 a, g dbSNP:73932617
695 695 a, g dbSNP:148839360
699 699 a, g dbSNP:537643737
702 702 c, t dbSNP:760312184
710 710 g, t dbSNP:768243223
711 711 a, c dbSNP:529790605
712 712 c, t dbSNP:199786442
713 713 a, g dbSNP:371037518
714 714 a, g dbSNP:766933442
716 716 a, g dbSNP:774699638
724 724 a, g dbSNP:759700116
725 725 c, t dbSNP:766283610
731 731 c, t dbSNP:767699197
732 732 c, t dbSNP:143518180
738 738 c, t dbSNP:756417848
751 751 a, g dbSNP:534688911
758 758 c, g dbSNP:753713626
760 760 c, t dbSNP:756951776
765 765 a, t dbSNP:778955963
772 772 c, t dbSNP:745792138
783 783 c, t dbSNP:757986467
786 786 c, t dbSNP:10401479
793 793 a, g dbSNP:201907046
797 797 c, t dbSNP:746453452
799 799 c, t dbSNP:368022457
800 800 a, g dbSNP:768329109
800 800 -, g dbSNP:756290566
801 801 a, g dbSNP:776216918
807 807 a, g dbSNP:200639337
827 827 a, g dbSNP:10401978
840 840 c, t dbSNP:575794794
841 841 a, g dbSNP:45588133
852 852 a, t dbSNP:561622740
912 912 c, t dbSNP:2659122
937 937 c, t dbSNP:764141650
943 943 a, g dbSNP:752451895
951 951 c, t dbSNP:574525519
976 976 a, g dbSNP:540652721
998 998 g, t dbSNP:778027460
1002 1002 c, t dbSNP:3209092
1017 1017 a, g dbSNP:559356703
1033 1033 c, t dbSNP:532978563
1070 1070 a, g dbSNP:771167591
1073 1073 a, g dbSNP:749612200
1083 1083 g, t dbSNP:529947489
1089 1089 a, c dbSNP:759946194
1090 1090 a, c dbSNP:768078108
1092 1092 c, t dbSNP:775712442
1096 1096 c, t dbSNP:760793589
1097 1097 a, c, t dbSNP:764431782
1100 1100 g, t dbSNP:757145681
1116 1116 a, t dbSNP:764929426
1117 1117 a, g dbSNP:151298874
1122 1122 -, t dbSNP:766453393
1124 1124 a, g dbSNP:750895817
1127 1127 g, t dbSNP:750179092
1128 1128 c, g dbSNP:549637095
1129 1129 a, c dbSNP:779926782
1139 1139 g, t dbSNP:746545348
1143 1143 c, t dbSNP:754464009
1146 1146 c, t dbSNP:140564011
1163 1163 c, t dbSNP:138882542
1164 1164 a, g, t dbSNP:61736309
1172 1172 a, g dbSNP:200116262
1176 1176 c, t dbSNP:772734530
1182 1182 a, g dbSNP:79245399
1192 1192 a, c dbSNP:144118163
1194 1194 c, t dbSNP:146297845
1195 1195 a, g dbSNP:372901531
1202 1202 c, t dbSNP:776973438
1203 1203 c, t dbSNP:45552633
1209 1209 a, g dbSNP:113126792
1219 1219 g, t dbSNP:538660698
1222 1222 g, t dbSNP:762688864
1228 1228 c, t dbSNP:1802720
1230 1230 c, t dbSNP:779407068
1231 1231 c, t dbSNP:746367915
1232 1232 a, g dbSNP:149376489
1240 1240 a, g, t dbSNP:548476435
1250 1250 c, t dbSNP:144748315
1252 1252 a, g dbSNP:752193560
1254 1254 c, t dbSNP:375475599
1255 1255 a, g, t dbSNP:200006942
1256 1256 a, t dbSNP:772540456
1264 1264 a, c, t dbSNP:780743219
1266 1266 c, t dbSNP:769230318
1269 1269 a, c dbSNP:776581784
1271 1271 c, t dbSNP:536820435
1279 1279 -, t dbSNP:753859707
1283 1283 c, t dbSNP:761917326
1284 1284 c, t dbSNP:1058205
1291 1291 c, t dbSNP:773462440
1292 1292 g, t dbSNP:762778769
1293 1293 c, t dbSNP:766160591
1294 1294 a, g dbSNP:373134993
1296 1296 c, t dbSNP:759482291
1299 1299 a, g dbSNP:374859717
1308 1308 c, t dbSNP:368035787
1309 1309 c, t dbSNP:752179012
1319 1319 c, g dbSNP:112479005
1328 1328 c, g dbSNP:141498977
1334 1334 a, g dbSNP:1058274
1336 1336 a, c dbSNP:749897325
1346 1346 a, t dbSNP:1803132
1358 1358 c, t dbSNP:1802722
1360 1360 c, t dbSNP:1141894
1367 1367 a, g dbSNP:577670828
1386 1386 c, t dbSNP:116064912
1393 1393 a, g dbSNP:1802721
1398 1398 c, t dbSNP:563949982
1405 1405 a, g dbSNP:116248649
1406 1406 a, c dbSNP:3180906
1418 1418 a, c dbSNP:569814469
1429 1429 c, g dbSNP:561507023
1431 1431 c, t dbSNP:16987771
1457 1457 c, t dbSNP:1803138
1469 1469 c, t dbSNP:572690444
1483 1483 a, g dbSNP:766084172
1510 1510 c, g dbSNP:190504941
1514 1514 c, g, t dbSNP:12101
1525 1525 c, g dbSNP:774491454
1538 1538 a, g dbSNP:546140331
1543 1543 c, t dbSNP:538641946
1547 1547 a, g dbSNP:6998
1549 1549 a, g dbSNP:569833730
1572 1572 g, t dbSNP:537315899
1575 1575 a, c dbSNP:16987775
1601 1601 c, t dbSNP:752217318
1647 1647 c, t dbSNP:555394775
1651 1651 c, t dbSNP:1803136
1656 1656 c, g dbSNP:573733138
1662 1662 c, t dbSNP:534527207
1669 1669 a, g dbSNP:1803135
1677 1677 g, t dbSNP:370827372
1682 1682 a, g dbSNP:193123776
1684 1684 a, g dbSNP:377210991
1689 1689 c, t dbSNP:1803134
1691 1691 a, c dbSNP:538849894
1713 1713 a, g dbSNP:12040
1729 1729 a, g dbSNP:61269009
1733 1733 g, t dbSNP:754156080
1739 1739 a, g dbSNP:556963317
1766 1766 c, t dbSNP:1803133
1773 1773 a, g dbSNP:11554029
1776 1776 a, g dbSNP:779131129
1784 1784 a, g dbSNP:12863
1801 1801 a, g dbSNP:750975726
1803 1803 a, g dbSNP:1803137
1808 1808 a, g dbSNP:11554028
1817 1817 -, g dbSNP:780652548
1817 1817 g, t dbSNP:575154555
1818 1818 c, g dbSNP:543260524
1858 1858 c, t dbSNP:11554027
1877 1877 c, t dbSNP:561435501
1902 1902 c, t dbSNP:758871345

Target ORF information:

RefSeq Version NM_001030047
Organism Homo sapiens (human)
Definition Homo sapiens kallikrein-related peptidase 3 (KLK3), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001030047

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGTGGGTCC CGGTTGTCTT CCTCACCCTG TCCGTGACGT GGATTGGTGC TGCACCCCTC 
ATCCTGTCTC GGATTGTGGG AGGCTGGGAG TGCGAGAAGC ATTCCCAACC CTGGCAGGTG
CTTGTGGCCT CTCGTGGCAG GGCAGTCTGC GGCGGTGTTC TGGTGCACCC CCAGTGGGTC
CTCACAGCTG CCCACTGCAT CAGGAACAAA AGCGTGATCT TGCTGGGTCG GCACAGCCTG
TTTCATCCTG AAGACACAGG CCAGGTATTT CAGGTCAGCC ACAGCTTCCC ACACCCGCTC
TACGATATGA GCCTCCTGAA GAATCGATTC CTCAGGCCAG GTGATGACTC CAGCCACGAC
CTCATGCTGC TCCGCCTGTC AGAGCCTGCC GAGCTCACGG ATGCTGTGAA GGTCATGGAC
CTGCCCACCC AGGAGCCAGC ACTGGGGACC ACCTGCTACG CCTCAGGCTG GGGCAGCATT
GAACCAGAGG AGTTCTTGAC CCCAAAGAAA CTTCAGTGTG TGGACCTCCA TGTTATTTCC
AATGACGTGT GTGCGCAAGT TCACCCTCAG AAGGTGACCA AGTTCATGCT GTGTGCTGGA
CGCTGGACAG GGGGCAAAAG CACCTGCTCG TGGGTCATTC TGATCACCGA ACTGACCATG
CCAGCCCTGC CGATGGTCCT CCATGGCTCC CTAGTGCCCT GGAGAGGAGG TGTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23926
Accession Version NM_001030048.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 657bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product prostate-specific antigen isoform 4 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC005307.1, BQ932072.1 and AC011523.3. Summary: Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (4) uses an alternate in-frame splice site at the end of a coding exon. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ932072.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147920, SAMEA2150385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGTGGGTCC CGGTTGTCTT CCTCACCCTG TCCGTGACGT GGATTGGTGC TGCACCCCTC 
ATCCTGTCTC GGATTGTGGG AGGCTGGGAG TGCGAGAAGC ATTCCCAACC CTGGCAGGTG
CTTGTGGCCT CTCGTGGCAG GGCAGTCTGC GGCGGTGTTC TGGTGCACCC CCAGTGGGTC
CTCACAGCTG CCCACTGCAT CAGGAAGCCA GGTGATGACT CCAGCCACGA CCTCATGCTG
CTCCGCCTGT CAGAGCCTGC CGAGCTCACG GATGCTGTGA AGGTCATGGA CCTGCCCACC
CAGGAGCCAG CACTGGGGAC CACCTGCTAC GCCTCAGGCT GGGGCAGCAT TGAACCAGAG
GAGTTCTTGA CCCCAAAGAA ACTTCAGTGT GTGGACCTCC ATGTTATTTC CAATGACGTG
TGTGCGCAAG TTCACCCTCA GAAGGTGACC AAGTTCATGC TGTGTGCTGG ACGCTGGACA
GGGGGCAAAA GCACCTGCTC GGGTGATTCT GGGGGCCCAC TTGTCTGTAA TGGTGTGCTT
CAAGGTATCA CGTCATGGGG CAGTGAACCA TGTGCCCTGC CCGAAAGGCC TTCCCTGTAC
ACCAAGGTGG TGCATTACCG GAAGTGGATC AAGGACACCA TCGTGGCCAA CCCCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001025219.1
CDS42..698
Misc Feature(1)111..671(+)
Misc Feature(2)114..680(+)
Misc Feature(3)114..116(+)
Misc Feature(4)234..551(+)
Misc Feature(5)531..602(+)
Exon (1)1..87
Gene:KLK3
Gene Synonym:
Exon (2)88..247
Gene:KLK3
Gene Synonym:
Exon (3)248..405
Gene:KLK3
Gene Synonym:
Exon (4)406..542
Gene:KLK3
Gene Synonym:
Exon (5)543..1335
Gene:KLK3
Gene Synonym:
Translation
Position Chain Variation Link
3 3 c, t dbSNP:371797932
7 7 a, c dbSNP:780950315
17 17 c, t dbSNP:370025467
21 21 c, t dbSNP:769236849
25 25 c, t dbSNP:199680464
26 26 a, g dbSNP:762592843
29 29 a, g dbSNP:751938250
40 40 c, t dbSNP:373854842
46 46 -, g dbSNP:779162374
50 50 c, t dbSNP:201712212
52 52 c, t dbSNP:111689449
53 53 a, g dbSNP:375104394
67 67 a, c dbSNP:368127664
73 73 a, c, t dbSNP:138400533
74 74 c, t dbSNP:765400712
75 75 a, g dbSNP:750712372
77 77 c, g dbSNP:758622865
79 79 c, t dbSNP:780090483
80 80 a, g dbSNP:751400607
87 87 g, t dbSNP:754663333
89 89 c, t dbSNP:17849961
89 89 c, t dbSNP:11573
91 91 c, t dbSNP:181375335
94 94 c, t dbSNP:750316227
95 95 a, g dbSNP:1135766
101 101 a, c dbSNP:765982632
111 111 c, t dbSNP:141576693
112 112 a, g dbSNP:147446084
115 115 c, t dbSNP:375110405
116 116 c, t dbSNP:754188902
118 118 a, t dbSNP:757579274
122 122 a, c dbSNP:200846924
125 125 c, t dbSNP:746365590
130 130 a, g, t dbSNP:772176502
131 131 a, g dbSNP:547221078
134 134 c, t dbSNP:139911579
135 135 a, g dbSNP:2271092
147 147 c, t dbSNP:747908647
150 150 c, t dbSNP:769617725
151 151 a, c dbSNP:773350836
154 154 g, t dbSNP:762952938
157 157 a, t dbSNP:200712655
158 158 a, g dbSNP:7252245
161 161 g, t dbSNP:568880974
163 163 c, t dbSNP:767083316
169 169 a, c dbSNP:140318583
170 170 c, g, t dbSNP:374094231
174 174 c, t dbSNP:555200638
175 175 a, g dbSNP:139560851
177 177 -, gg dbSNP:775070174
181 181 a, g dbSNP:758925761
183 183 a, g dbSNP:149709822
186 186 c, g dbSNP:747094712
187 187 -, a dbSNP:34953540
191 191 c, t dbSNP:144294681
192 192 c, g dbSNP:781327047
194 194 -, ggg dbSNP:375428882
194 194 c, t dbSNP:201710884
195 195 a, g dbSNP:369223448
204 204 a, g dbSNP:773127519
207 207 c, t dbSNP:577778784
209 209 c, g dbSNP:770994770
211 211 c, t dbSNP:545098001
212 212 c, g dbSNP:758987002
213 213 a, c, t dbSNP:767027476
216 216 g, t dbSNP:760366035
221 221 c, t dbSNP:763682700
224 224 c, t dbSNP:750794289
237 237 c, t dbSNP:371595805
249 249 c, g dbSNP:776101817
252 252 g, t dbSNP:574935823
253 253 a, g dbSNP:764323663
258 258 a, g dbSNP:776860388
259 259 a, c dbSNP:761947130
261 261 c, t dbSNP:765521022
269 269 c, g, t dbSNP:377532899
270 270 a, g dbSNP:766084011
272 272 c, t dbSNP:542099924
273 273 c, t dbSNP:754921426
282 282 c, t dbSNP:201390347
285 285 c, t dbSNP:61750343
286 286 a, g dbSNP:755527546
297 297 a, c, t dbSNP:527812439
302 302 c, t dbSNP:201110725
303 303 a, g, t dbSNP:182759459
306 306 a, c, t dbSNP:2003783
310 310 c, t dbSNP:373661565
311 311 a, g dbSNP:765201777
316 316 c, t dbSNP:773552177
318 318 a, g, t dbSNP:149427770
320 320 a, g dbSNP:751359189
322 322 a, g dbSNP:754728036
328 328 g, t dbSNP:767463542
338 338 c, t dbSNP:752607029
359 359 a, g, t dbSNP:147547638
368 368 c, t dbSNP:753580440
371 371 c, t dbSNP:376483907
372 372 a, g dbSNP:138273452
374 374 -, t dbSNP:756135278
386 386 a, c, t dbSNP:747404815
388 388 a, g dbSNP:781775024
390 390 a, t dbSNP:200126385
392 392 g, t dbSNP:748662283
396 396 c, t dbSNP:112372888
401 401 a, g dbSNP:143868213
404 404 c, g dbSNP:763023030
405 405 g, t dbSNP:771089837
406 406 c, t dbSNP:778191056
407 407 a, c, t dbSNP:749770722
412 412 c, t dbSNP:367976209
415 415 c, t dbSNP:745885851
424 424 a, t dbSNP:772282891
430 430 a, g dbSNP:775735844
437 437 c, t dbSNP:202206082
440 440 c, g dbSNP:148735869
441 441 c, t dbSNP:776501538
443 443 c, t dbSNP:372190695
448 448 c, t dbSNP:17632542
457 457 a, t dbSNP:772853400
458 458 a, c, t dbSNP:372148450
459 459 a, g dbSNP:150769385
466 466 c, t dbSNP:554107787
467 467 a, g dbSNP:376510386
475 475 a, g dbSNP:759706621
479 479 a, t dbSNP:572619039
486 486 a, g, t dbSNP:376934756
495 495 a, t dbSNP:745974856
500 500 c, g dbSNP:139172057
503 503 c, g dbSNP:758455175
505 505 g, t dbSNP:780184499
512 512 a, c, g dbSNP:1803129
513 513 c, t dbSNP:546294568
515 515 c, t dbSNP:370658603
517 517 g, t dbSNP:187449592
518 518 g, t dbSNP:191588971
520 520 a, c, t dbSNP:146528783
526 526 a, g dbSNP:762600618
541 541 c, g, t dbSNP:61729813
542 542 a, g dbSNP:759209785
545 545 a, t dbSNP:764929426
546 546 a, g dbSNP:151298874
551 551 -, t dbSNP:766453393
553 553 a, g dbSNP:750895817
556 556 g, t dbSNP:750179092
557 557 c, g dbSNP:549637095
558 558 a, c dbSNP:779926782
568 568 g, t dbSNP:746545348
572 572 c, t dbSNP:754464009
575 575 c, t dbSNP:140564011
592 592 c, t dbSNP:138882542
593 593 a, g, t dbSNP:61736309
601 601 a, g dbSNP:200116262
605 605 c, t dbSNP:772734530
611 611 a, g dbSNP:79245399
621 621 a, c dbSNP:144118163
623 623 c, t dbSNP:146297845
624 624 a, g dbSNP:372901531
631 631 c, t dbSNP:776973438
632 632 c, t dbSNP:45552633
638 638 a, g dbSNP:113126792
648 648 g, t dbSNP:538660698
651 651 g, t dbSNP:762688864
657 657 c, t dbSNP:1802720
659 659 c, t dbSNP:779407068
660 660 c, t dbSNP:746367915
661 661 a, g dbSNP:149376489
669 669 a, g, t dbSNP:548476435
679 679 c, t dbSNP:144748315
681 681 a, g dbSNP:752193560
683 683 c, t dbSNP:375475599
684 684 a, g, t dbSNP:200006942
685 685 a, t dbSNP:772540456
693 693 a, c, t dbSNP:780743219
695 695 c, t dbSNP:769230318
698 698 a, c dbSNP:776581784
700 700 c, t dbSNP:536820435
708 708 -, t dbSNP:753859707
712 712 c, t dbSNP:761917326
713 713 c, t dbSNP:1058205
720 720 c, t dbSNP:773462440
721 721 g, t dbSNP:762778769
722 722 c, t dbSNP:766160591
723 723 a, g dbSNP:373134993
725 725 c, t dbSNP:759482291
728 728 a, g dbSNP:374859717
737 737 c, t dbSNP:368035787
738 738 c, t dbSNP:752179012
748 748 c, g dbSNP:112479005
757 757 c, g dbSNP:141498977
763 763 a, g dbSNP:1058274
765 765 a, c dbSNP:749897325
775 775 a, t dbSNP:1803132
787 787 c, t dbSNP:1802722
789 789 c, t dbSNP:1141894
796 796 a, g dbSNP:577670828
815 815 c, t dbSNP:116064912
822 822 a, g dbSNP:1802721
827 827 c, t dbSNP:563949982
834 834 a, g dbSNP:116248649
835 835 a, c dbSNP:3180906
847 847 a, c dbSNP:569814469
858 858 c, g dbSNP:561507023
860 860 c, t dbSNP:16987771
886 886 c, t dbSNP:1803138
898 898 c, t dbSNP:572690444
912 912 a, g dbSNP:766084172
939 939 c, g dbSNP:190504941
943 943 c, g, t dbSNP:12101
954 954 c, g dbSNP:774491454
967 967 a, g dbSNP:546140331
972 972 c, t dbSNP:538641946
976 976 a, g dbSNP:6998
978 978 a, g dbSNP:569833730
1001 1001 g, t dbSNP:537315899
1004 1004 a, c dbSNP:16987775
1030 1030 c, t dbSNP:752217318
1076 1076 c, t dbSNP:555394775
1080 1080 c, t dbSNP:1803136
1085 1085 c, g dbSNP:573733138
1091 1091 c, t dbSNP:534527207
1098 1098 a, g dbSNP:1803135
1106 1106 g, t dbSNP:370827372
1111 1111 a, g dbSNP:193123776
1113 1113 a, g dbSNP:377210991
1118 1118 c, t dbSNP:1803134
1120 1120 a, c dbSNP:538849894
1142 1142 a, g dbSNP:12040
1158 1158 a, g dbSNP:61269009
1162 1162 g, t dbSNP:754156080
1168 1168 a, g dbSNP:556963317
1195 1195 c, t dbSNP:1803133
1202 1202 a, g dbSNP:11554029
1205 1205 a, g dbSNP:779131129
1213 1213 a, g dbSNP:12863
1230 1230 a, g dbSNP:750975726
1232 1232 a, g dbSNP:1803137
1237 1237 a, g dbSNP:11554028
1246 1246 -, g dbSNP:780652548
1246 1246 g, t dbSNP:575154555
1247 1247 c, g dbSNP:543260524
1287 1287 c, t dbSNP:11554027
1306 1306 c, t dbSNP:561435501
1331 1331 c, t dbSNP:758871345

Target ORF information:

RefSeq Version NM_001030048
Organism Homo sapiens (human)
Definition Homo sapiens kallikrein-related peptidase 3 (KLK3), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001030048

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGTGGGTCC CGGTTGTCTT CCTCACCCTG TCCGTGACGT GGATTGGTGC TGCACCCCTC 
ATCCTGTCTC GGATTGTGGG AGGCTGGGAG TGCGAGAAGC ATTCCCAACC CTGGCAGGTG
CTTGTGGCCT CTCGTGGCAG GGCAGTCTGC GGCGGTGTTC TGGTGCACCC CCAGTGGGTC
CTCACAGCTG CCCACTGCAT CAGGAAGCCA GGTGATGACT CCAGCCACGA CCTCATGCTG
CTCCGCCTGT CAGAGCCTGC CGAGCTCACG GATGCTGTGA AGGTCATGGA CCTGCCCACC
CAGGAGCCAG CACTGGGGAC CACCTGCTAC GCCTCAGGCT GGGGCAGCAT TGAACCAGAG
GAGTTCTTGA CCCCAAAGAA ACTTCAGTGT GTGGACCTCC ATGTTATTTC CAATGACGTG
TGTGCGCAAG TTCACCCTCA GAAGGTGACC AAGTTCATGC TGTGTGCTGG ACGCTGGACA
GGGGGCAAAA GCACCTGCTC GGGTGATTCT GGGGGCCCAC TTGTCTGTAA TGGTGTGCTT
CAAGGTATCA CGTCATGGGG CAGTGAACCA TGTGCCCTGC CCGAAAGGCC TTCCCTGTAC
ACCAAGGTGG TGCATTACCG GAAGTGGATC AAGGACACCA TCGTGGCCAA CCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.