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IL6 interleukin 6 [Homo sapiens (human)]

Gene Symbol IL6
Entrez Gene ID 3569
Full Name interleukin 6
Synonyms BSF2, HGF, HSF, IFNB2, IL-6
General protein information
Preferred Names
interleukin-6
Names
interleukin-6
CDF
BSF-2
IFN-beta-2
interferon beta-2
interleukin BSF-2
hybridoma growth factor
CTL differentiation factor
B-cell stimulatory factor 2
B-cell differentiation factor
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7p21

Summary This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011]. lac of sum
Disorder MIM:

147620

Disorder Html: {Rheumatoid arthritis, systemic juvenile}, 604302 (3); {Kaposi





The following IL6 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IL6 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu41176 XM_005249745 PREDICTED: Homo sapiens interleukin 6 (IL6), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu23477 XM_011515390 PREDICTED: Homo sapiens interleukin 6 (IL6), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$199
OHu58395 XM_011515391 PREDICTED: Homo sapiens interleukin 6 (IL6), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $99
OHu23477 NM_000600 Homo sapiens interleukin 6 (IL6), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$269

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu41176
Accession Version XM_005249745.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 759bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product interleukin-6 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007819.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578813494. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGAACTCCT TCTCCACAAG TAAGTGCAGG AAATCCTTAG CCCTGGAACT GCCAGCGGCG 
GTCGAGCCCT GTGTGAGGGA GGGGTGTGTG GCCCAGGGAG GGCTGGCGGG CGGCCAGCAG
CAGAGGCAGG CTCCCAGCTG TGCTGTCAGC TCACCCCTGC GCTCGCTCCC CTCCGGCACA
GGCGCCTTCG GTCCAGTTGC CTTCTCCCTG GGGCTGCTCC TGGTGTTGCC TGCTGCCTTC
CCTGCCCCAG TACCCCCAGG AGAAGATTCC AAAGATGTAG CCGCCCCACA CAGACAGCCA
CTCACCTCTT CAGAACGAAT TGACAAACAA ATTCGGTACA TCCTCGACGG CATCTCAGCC
CTGAGAAAGG AGACATGTAA CAAGAGTAAC ATGTGTGAAA GCAGCAAAGA GGCACTGGCA
GAAAACAACC TGAACCTTCC AAAGATGGCT GAAAAAGATG GATGCTTCCA ATCTGGATTC
AATGAGGAGA CTTGCCTGGT GAAAATCATC ACTGGTCTTT TGGAGTTTGA GGTATACCTA
GAGTACCTCC AGAACAGATT TGAGAGTAGT GAGGAACAAG CCAGAGCTGT GCAGATGAGT
ACAAAAGTCC TGATCCAGTT CCTGCAGAAA AAGGTGGGTG TGTCCTCATT CCCTCAACTT
GGTGTGGGGG AAGACAGGCT CAAAGACAGT GTCCTGGACA ACTCAGGGAT GCAATGCCAC
TTCCAAAAGA GAAGGCTACA CGTAAACAAA AGAGTCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005249802.1
CDS184..942
Misc Feature(1)514..>819(+)
Translation
Position Chain Variation Link
10 10 g, t dbSNP:13447446
36 36 c, t dbSNP:527770772
58 58 c, g dbSNP:13447445
60 60 g, t dbSNP:200292679
84 84 a, c dbSNP:201575115
108 108 a, g dbSNP:779901687
128 128 c, t dbSNP:199671830
134 134 g, t dbSNP:13306438
136 136 a, c dbSNP:2069857
138 138 a, g dbSNP:758839425
140 140 a, c dbSNP:185230192
141 141 a, g dbSNP:199546667
141 141 -, g dbSNP:778200235
145 145 a, g dbSNP:777978147
147 147 a, c, g dbSNP:746737342
151 151 c, g dbSNP:3087226
161 161 a, g dbSNP:368772259
162 162 c, t dbSNP:745397994
163 163 c, t dbSNP:769242976
172 172 a, c dbSNP:775434886
183 183 -, t dbSNP:745672376
188 188 a, t dbSNP:200700194
189 189 c, t dbSNP:768540611
192 192 c, t dbSNP:200815144
199 199 a, g dbSNP:569143621
200 200 c, t dbSNP:774003579
204 204 c, t dbSNP:761024140
208 208 g, t dbSNP:766513767
209 209 a, g dbSNP:776934129
211 211 a, c dbSNP:759723222
213 213 a, g dbSNP:765378428
221 221 c, t dbSNP:778852405
224 224 c, t dbSNP:758819517
230 230 a, t dbSNP:764590152
234 234 a, g dbSNP:751878349
240 240 a, g dbSNP:757587594
243 243 a, g dbSNP:780848732
245 245 a, g, t dbSNP:199719913
246 246 a, c dbSNP:55927793
247 247 a, g, t dbSNP:551377783
250 250 c, g, t dbSNP:755658631
253 253 a, t dbSNP:749300991
254 254 c, g dbSNP:769639272
267 267 a, g, t dbSNP:768473558
269 269 a, g dbSNP:747908591
274 274 a, g dbSNP:771943009
276 276 c, t dbSNP:777036221
279 279 a, g dbSNP:571307563
281 281 c, g dbSNP:533650970
285 285 a, g dbSNP:775712169
291 291 a, g dbSNP:771313761
294 294 c, g, t dbSNP:764525148
300 300 a, g dbSNP:757591276
301 301 c, t dbSNP:767834140
310 310 c, g dbSNP:750121043
311 311 a, t dbSNP:553784698
317 317 a, c dbSNP:779605333
320 320 a, g dbSNP:748751521
322 322 a, c, t dbSNP:376047818
344 344 g, t dbSNP:748090079
348 348 g, t dbSNP:370286060
349 349 c, t dbSNP:773127971
355 355 c, t dbSNP:746159740
356 356 c, t dbSNP:770150376
358 358 c, g dbSNP:201493844
362 362 c, g, t dbSNP:201776690
366 366 a, c dbSNP:199941251
369 369 a, c dbSNP:200637969
374 374 c, g dbSNP:762283178
382 382 g, t dbSNP:767924065
383 383 c, t dbSNP:750778143
386 386 -, tc dbSNP:779919594
387 387 a, c dbSNP:760962452
388 388 -, c dbSNP:34280821
393 393 g, t dbSNP:766033770
397 397 c, t dbSNP:753479461
405 405 a, g dbSNP:201852608
409 409 c, t dbSNP:148516576
415 415 c, g dbSNP:746848366
436 436 a, c dbSNP:142759801
437 437 c, g dbSNP:778465778
439 439 c, t dbSNP:2069830
441 441 a, g dbSNP:758406611
449 449 a, t dbSNP:201787553
461 461 c, t dbSNP:769904935
466 466 a, g dbSNP:746818346
469 469 c, g dbSNP:770802865
473 473 a, g dbSNP:780174319
475 475 a, c dbSNP:199957016
480 480 g, t dbSNP:544833266
488 488 c, t dbSNP:768665469
499 499 a, c dbSNP:774472593
503 503 c, t dbSNP:762371056
508 508 a, g dbSNP:202138427
509 509 a, g dbSNP:541074925
513 513 a, c dbSNP:199925567
519 519 a, g dbSNP:200999878
520 520 c, t dbSNP:201822486
525 525 a, c dbSNP:753484746
528 528 c, t dbSNP:140764737
531 531 c, t dbSNP:764720869
532 532 a, g, t dbSNP:752257060
540 540 a, c dbSNP:201410817
558 558 a, g, t dbSNP:145242107
559 559 c, t dbSNP:370818995
561 561 c, t dbSNP:147625275
566 566 a, g dbSNP:746269216
573 573 c, t dbSNP:770018245
574 574 a, g dbSNP:201121373
575 575 c, t dbSNP:775325818
580 580 c, g dbSNP:190436077
582 582 a, g dbSNP:200987147
585 585 c, t dbSNP:201523057
594 594 a, c, g, t dbSNP:142164099
595 595 a, g dbSNP:761157550
603 603 a, c dbSNP:375470014
605 605 a, g dbSNP:199990564
612 612 c, t dbSNP:750278000
616 616 a, t dbSNP:755968353
617 617 a, t dbSNP:200745306
618 618 c, t dbSNP:766129888
630 630 c, g dbSNP:752964616
640 640 c, g dbSNP:758616001
645 645 a, t dbSNP:777906302
653 653 a, t dbSNP:747126003
654 654 a, g dbSNP:757236211
656 656 a, c dbSNP:202210223
665 665 a, g dbSNP:781740062
671 671 a, g dbSNP:200263800
672 672 a, g dbSNP:751000694
679 679 c, g dbSNP:756681741
681 681 a, g dbSNP:780638841
691 691 a, t dbSNP:148171375
700 700 c, g dbSNP:769089379
701 701 c, t dbSNP:11544633
704 704 c, t dbSNP:778738185
715 715 a, g dbSNP:371302236
716 716 c, t dbSNP:771660418
726 726 g, t dbSNP:772582867
728 728 a, g dbSNP:201371019
735 735 c, g dbSNP:760165722
737 737 a, g dbSNP:770868572
743 743 a, t dbSNP:776504834
747 747 a, g dbSNP:759359627
751 751 a, g dbSNP:56383910
756 756 c, g dbSNP:764988588
767 767 a, g, t dbSNP:751859032
770 770 a, c dbSNP:767699802
791 791 a, t dbSNP:750469507
797 797 -, c dbSNP:34012176
801 801 c, g dbSNP:182812860
804 804 c, t dbSNP:199569510
809 809 a, g dbSNP:780726676
819 819 a, c, g, t dbSNP:754355352
821 821 a, g dbSNP:747891634
823 823 a, g dbSNP:375914665
824 824 c, t dbSNP:777425937
826 826 c, t dbSNP:746547335
828 828 a, c dbSNP:770390140
834 834 c, t dbSNP:201024011
837 837 a, t dbSNP:369156051
838 838 c, t dbSNP:200717293
840 840 a, g dbSNP:200598794
841 841 c, t dbSNP:779921929
845 845 a, g dbSNP:762738660
847 847 a, g dbSNP:2069842
848 848 g, t dbSNP:749356424
849 849 a, g dbSNP:773431914
852 852 c, g dbSNP:375034741
860 860 g, t dbSNP:764836798
861 861 c, g dbSNP:199600878
873 873 c, t dbSNP:766533471
877 877 c, t dbSNP:754443129
882 882 c, g dbSNP:755528306
885 885 c, g dbSNP:765699232
886 886 g, t dbSNP:752190332
889 889 c, g dbSNP:753017017
890 890 a, g dbSNP:758741386
901 901 c, t dbSNP:143808144
902 902 a, c dbSNP:539937012
911 911 a, g dbSNP:746635233
912 912 c, g dbSNP:756811740
921 921 a, g dbSNP:553150365
924 924 c, t dbSNP:116693967
925 925 a, g dbSNP:758370297
930 930 c, t dbSNP:775435569
931 931 -, aa dbSNP:773977658
932 932 a, g dbSNP:749056272
935 935 a, g dbSNP:768768787
937 937 c, g dbSNP:774522493
938 938 a, t dbSNP:761980206
939 939 a, c dbSNP:772203762
941 941 c, g dbSNP:773560775
946 946 a, c dbSNP:768454698
947 947 c, t dbSNP:761014545
949 949 c, g dbSNP:773345367
971 971 c, t dbSNP:534489769
972 972 -, t dbSNP:747711886
977 977 -, tttg dbSNP:771704752
985 985 c, t dbSNP:766623368
1027 1027 c, t dbSNP:540172221
1036 1036 a, c dbSNP:368296317
1054 1054 a, g dbSNP:147239250
1102 1102 -, g dbSNP:199500690
1112 1112 a, g dbSNP:185681377
1244 1244 c, t dbSNP:562939646
1248 1248 -, t dbSNP:760646760
1301 1301 c, g dbSNP:112538818
1302 1302 g, t dbSNP:191229975
1310 1310 c, g dbSNP:1548216
1379 1379 -, c dbSNP:536120716
1404 1404 a, g dbSNP:140747517
1414 1414 c, t dbSNP:559151534
1415 1415 g, t dbSNP:762521592
1443 1443 a, t dbSNP:182599553
1444 1444 a, g dbSNP:144709966
1516 1516 -, g dbSNP:762618439
1531 1531 a, g dbSNP:2069843
1547 1547 a, c dbSNP:2069844
1559 1559 a, g dbSNP:551344842
1563 1563 c, t dbSNP:571166016
1585 1585 a, g dbSNP:756910594
1599 1599 c, t dbSNP:564355559
1602 1602 c, t dbSNP:150863637
1640 1640 a, g dbSNP:778965599
1643 1643 a, g dbSNP:546993286
1681 1681 c, t dbSNP:748114000
1686 1686 a, g dbSNP:2069845
1708 1708 c, t dbSNP:535975795
1719 1719 c, t dbSNP:2069859
1766 1766 a, g dbSNP:750228073
1794 1794 g, t dbSNP:139543282
1796 1796 a, g dbSNP:536848591
1820 1820 c, t dbSNP:556798892
1860 1860 a, g dbSNP:778115500
1876 1876 a, c dbSNP:576745033
1882 1882 a, g, t dbSNP:545455912
1888 1888 c, t dbSNP:149720994
1899 1899 a, g dbSNP:2069846
1997 1997 c, t dbSNP:375849717
2048 2048 c, g dbSNP:759268329
2059 2059 c, t dbSNP:535696222
2070 2070 c, t dbSNP:55912024
2144 2144 c, t dbSNP:762330834
2145 2145 a, g dbSNP:2069847
2156 2156 a, c dbSNP:116290445
2157 2157 c, t dbSNP:564740423
2164 2164 g, t dbSNP:540205400
2218 2218 c, t dbSNP:374414142
2249 2249 g, t dbSNP:546661339
2274 2274 a, t dbSNP:2069848
2292 2292 a, g dbSNP:56334855
2294 2294 g, t dbSNP:566896474
2304 2304 g, t dbSNP:529363262
2317 2317 a, c dbSNP:193120697
2339 2339 a, c dbSNP:569016990
2369 2369 c, g dbSNP:536817770
2383 2383 -, a dbSNP:747195700
2383 2383 a, g dbSNP:556761977
2384 2384 a, c dbSNP:79785364
2416 2416 a, g dbSNP:570393926
2472 2472 c, t dbSNP:539757735
2476 2476 c, t dbSNP:111899782
2514 2514 a, g dbSNP:776846134
2525 2525 a, c dbSNP:759632195
2531 2531 a, g dbSNP:765685478
2537 2537 a, c, g dbSNP:753209562
2540 2540 c, t dbSNP:764500077
2543 2543 -, a dbSNP:772670999
2544 2544 a, g dbSNP:751273236
2547 2547 g, t dbSNP:756903277
2552 2552 c, t dbSNP:780759201

Target ORF information:

RefSeq Version XM_005249745
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens interleukin 6 (IL6), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005249745

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGAACTCCT TCTCCACAAG TAAGTGCAGG AAATCCTTAG CCCTGGAACT GCCAGCGGCG 
GTCGAGCCCT GTGTGAGGGA GGGGTGTGTG GCCCAGGGAG GGCTGGCGGG CGGCCAGCAG
CAGAGGCAGG CTCCCAGCTG TGCTGTCAGC TCACCCCTGC GCTCGCTCCC CTCCGGCACA
GGCGCCTTCG GTCCAGTTGC CTTCTCCCTG GGGCTGCTCC TGGTGTTGCC TGCTGCCTTC
CCTGCCCCAG TACCCCCAGG AGAAGATTCC AAAGATGTAG CCGCCCCACA CAGACAGCCA
CTCACCTCTT CAGAACGAAT TGACAAACAA ATTCGGTACA TCCTCGACGG CATCTCAGCC
CTGAGAAAGG AGACATGTAA CAAGAGTAAC ATGTGTGAAA GCAGCAAAGA GGCACTGGCA
GAAAACAACC TGAACCTTCC AAAGATGGCT GAAAAAGATG GATGCTTCCA ATCTGGATTC
AATGAGGAGA CTTGCCTGGT GAAAATCATC ACTGGTCTTT TGGAGTTTGA GGTATACCTA
GAGTACCTCC AGAACAGATT TGAGAGTAGT GAGGAACAAG CCAGAGCTGT GCAGATGAGT
ACAAAAGTCC TGATCCAGTT CCTGCAGAAA AAGGTGGGTG TGTCCTCATT CCCTCAACTT
GGTGTGGGGG AAGACAGGCT CAAAGACAGT GTCCTGGACA ACTCAGGGAT GCAATGCCAC
TTCCAAAAGA GAAGGCTACA CGTAAACAAA AGAGTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23477
Accession Version XM_011515390.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 639bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu23477D_COA.pdf (pdf)
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product interleukin-6 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007819.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGAACTCCT TCTCCACAAG CGCCTTCGGT CCAGTTGCCT TCTCCCTGGG GCTGCTCCTG 
GTGTTGCCTG CTGCCTTCCC TGCCCCAGTA CCCCCAGGAG AAGATTCCAA AGATGTAGCC
GCCCCACACA GACAGCCACT CACCTCTTCA GAACGAATTG ACAAACAAAT TCGGTACATC
CTCGACGGCA TCTCAGCCCT GAGAAAGGAG ACATGTAACA AGAGTAACAT GTGTGAAAGC
AGCAAAGAGG CACTGGCAGA AAACAACCTG AACCTTCCAA AGATGGCTGA AAAAGATGGA
TGCTTCCAAT CTGGATTCAA TGAGGAGACT TGCCTGGTGA AAATCATCAC TGGTCTTTTG
GAGTTTGAGG TATACCTAGA GTACCTCCAG AACAGATTTG AGAGTAGTGA GGAACAAGCC
AGAGCTGTGC AGATGAGTAC AAAAGTCCTG ATCCAGTTCC TGCAGAAAAA GGCAAAGAAT
CTAGATGCAA TAACCACCCC TGACCCAACC ACAAATGCCA GCCTGCTGAC GAAGCTGCAG
GCACAGAACC AGTGGCTGCA GGACATGACA ACTCATCTCA TTCTGCGCAG CTTTAAGGAG
TTCCTGCAGT CCAGCCTGAG GGCTCTTCGG CAAATGTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011513692.1
CDS902..1540
Misc Feature(1)1070..1531(+)
Translation
Position Chain Variation Link
11 11 a, g dbSNP:575234079
26 26 c, t dbSNP:544362915
42 42 g, t dbSNP:564666554
43 43 g, t dbSNP:533516234
87 87 c, t dbSNP:113950852
95 95 c, t dbSNP:560586738
105 105 a, t dbSNP:529611399
109 109 c, t dbSNP:190317882
113 113 c, t dbSNP:549089696
172 172 c, t dbSNP:2069824
177 177 a, g dbSNP:141571913
183 183 c, t dbSNP:150917469
205 205 c, t dbSNP:770990939
221 221 a, t dbSNP:536666227
228 228 c, t dbSNP:571412332
261 261 c, t dbSNP:756828021
275 275 c, t dbSNP:371227313
276 276 -, tc dbSNP:35081782
276 276 c, g dbSNP:374295772
277 277 -, ct, tc dbSNP:2069825
280 280 -, ct dbSNP:368171275
281 281 -, ct dbSNP:397766885
296 296 g, t dbSNP:539208095
300 300 g, t dbSNP:780685481
301 301 a, t dbSNP:745325279
313 313 c, g dbSNP:558674961
316 316 c, t dbSNP:769641085
351 351 a, g dbSNP:2069826
372 372 c, t dbSNP:573026666
396 396 g, t dbSNP:2069827
407 407 a, g dbSNP:201265474
409 409 a, t dbSNP:201997854
413 413 c, t dbSNP:199942883
422 422 a, g dbSNP:200851636
445 445 a, g dbSNP:115012815
476 476 c, g dbSNP:200466757
488 488 c, g dbSNP:182580364
490 490 a, t dbSNP:202025278
510 510 a, g dbSNP:557844653
520 520 -, tgtctggg dbSNP:575108248
543 543 a, g dbSNP:200247322
548 548 a, g dbSNP:201568050
550 550 c, t dbSNP:199708626
558 558 a, g dbSNP:200253893
560 560 a, c dbSNP:201148692
562 562 c, t dbSNP:199603107
586 586 c, t dbSNP:139228648
628 628 a, c dbSNP:768545676
632 632 a, c dbSNP:202025448
655 655 a, g dbSNP:199598337
665 665 c, t dbSNP:200709177
675 675 c, g dbSNP:3087223
681 681 g, t dbSNP:201847651
686 686 g, t dbSNP:766655687
691 691 a, g dbSNP:200123594
696 696 a, t dbSNP:201126898
699 699 a, g dbSNP:560763141
700 700 c, t dbSNP:3087224
703 703 a, g dbSNP:529548126
706 706 c, g dbSNP:201719180
713 713 c, t dbSNP:199884266
724 724 c, t dbSNP:543348874
732 732 -, a dbSNP:2069828
732 732 a, c dbSNP:201211345
734 734 -, a dbSNP:397897308
736 736 a, c dbSNP:201199436
746 746 a, g dbSNP:775464662
759 759 a, g dbSNP:200322276
784 784 c, t dbSNP:145356118
805 805 a, c dbSNP:75507584
826 826 a, g dbSNP:779901687
846 846 c, t dbSNP:199671830
852 852 g, t dbSNP:13306438
854 854 a, c dbSNP:2069857
856 856 a, g dbSNP:758839425
858 858 a, c dbSNP:185230192
859 859 a, g dbSNP:199546667
859 859 -, g dbSNP:778200235
863 863 a, g dbSNP:777978147
865 865 a, c, g dbSNP:746737342
869 869 c, g dbSNP:3087226
879 879 a, g dbSNP:368772259
880 880 c, t dbSNP:745397994
881 881 c, t dbSNP:769242976
890 890 a, c dbSNP:775434886
901 901 -, t dbSNP:745672376
906 906 a, t dbSNP:200700194
907 907 c, t dbSNP:768540611
910 910 c, t dbSNP:200815144
917 917 a, g dbSNP:569143621
918 918 c, t dbSNP:774003579
922 922 a, c dbSNP:199941251
925 925 a, c dbSNP:200637969
930 930 c, g dbSNP:762283178
938 938 g, t dbSNP:767924065
939 939 c, t dbSNP:750778143
942 942 -, tc dbSNP:779919594
943 943 a, c dbSNP:760962452
944 944 -, c dbSNP:34280821
949 949 g, t dbSNP:766033770
953 953 c, t dbSNP:753479461
961 961 a, g dbSNP:201852608
965 965 c, t dbSNP:148516576
971 971 c, g dbSNP:746848366
992 992 a, c dbSNP:142759801
993 993 c, g dbSNP:778465778
995 995 c, t dbSNP:2069830
997 997 a, g dbSNP:758406611
1005 1005 a, t dbSNP:201787553
1017 1017 c, t dbSNP:769904935
1022 1022 a, g dbSNP:746818346
1025 1025 c, g dbSNP:770802865
1029 1029 a, g dbSNP:780174319
1031 1031 a, c dbSNP:199957016
1036 1036 g, t dbSNP:544833266
1044 1044 c, t dbSNP:768665469
1055 1055 a, c dbSNP:774472593
1059 1059 c, t dbSNP:762371056
1064 1064 a, g dbSNP:202138427
1065 1065 a, g dbSNP:541074925
1069 1069 a, c dbSNP:199925567
1075 1075 a, g dbSNP:200999878
1076 1076 c, t dbSNP:201822486
1081 1081 a, c dbSNP:753484746
1084 1084 c, t dbSNP:140764737
1087 1087 c, t dbSNP:764720869
1088 1088 a, g, t dbSNP:752257060
1096 1096 a, c dbSNP:201410817
1114 1114 a, g, t dbSNP:145242107
1115 1115 c, t dbSNP:370818995
1117 1117 c, t dbSNP:147625275
1122 1122 a, g dbSNP:746269216
1129 1129 c, t dbSNP:770018245
1130 1130 a, g dbSNP:201121373
1131 1131 c, t dbSNP:775325818
1136 1136 c, g dbSNP:190436077
1138 1138 a, g dbSNP:200987147
1141 1141 c, t dbSNP:201523057
1150 1150 a, c, g, t dbSNP:142164099
1151 1151 a, g dbSNP:761157550
1159 1159 a, c dbSNP:375470014
1161 1161 a, g dbSNP:199990564
1168 1168 c, t dbSNP:750278000
1172 1172 a, t dbSNP:755968353
1173 1173 a, t dbSNP:200745306
1174 1174 c, t dbSNP:766129888
1186 1186 c, g dbSNP:752964616
1196 1196 c, g dbSNP:758616001
1201 1201 a, t dbSNP:777906302
1209 1209 a, t dbSNP:747126003
1210 1210 a, g dbSNP:757236211
1212 1212 a, c dbSNP:202210223
1221 1221 a, g dbSNP:781740062
1227 1227 a, g dbSNP:200263800
1228 1228 a, g dbSNP:751000694
1235 1235 c, g dbSNP:756681741
1237 1237 a, g dbSNP:780638841
1247 1247 a, t dbSNP:148171375
1256 1256 c, g dbSNP:769089379
1257 1257 c, t dbSNP:11544633
1260 1260 c, t dbSNP:778738185
1271 1271 a, g dbSNP:371302236
1272 1272 c, t dbSNP:771660418
1282 1282 g, t dbSNP:772582867
1284 1284 a, g dbSNP:201371019
1291 1291 c, g dbSNP:760165722
1293 1293 a, g dbSNP:770868572
1299 1299 a, t dbSNP:776504834
1303 1303 a, g dbSNP:759359627
1307 1307 a, g dbSNP:56383910
1312 1312 c, g dbSNP:764988588
1323 1323 a, g, t dbSNP:751859032
1326 1326 a, c dbSNP:767699802
1347 1347 a, t dbSNP:750469507
1353 1353 -, c dbSNP:34012176
1357 1357 c, g dbSNP:182812860
1360 1360 c, t dbSNP:199569510
1365 1365 a, g dbSNP:780726676
1378 1378 c, g, t dbSNP:200477775
1383 1383 c, t dbSNP:369953112
1386 1386 a, t dbSNP:2069860
1387 1387 a, t dbSNP:13306435
1388 1388 c, g dbSNP:768383637
1389 1389 c, t dbSNP:778836162
1397 1397 a, c dbSNP:747302620
1398 1398 c, t dbSNP:771282862
1399 1399 c, t dbSNP:541464782
1400 1400 c, g dbSNP:200641450
1405 1405 a, c, g dbSNP:201947383
1406 1406 c, t dbSNP:769888477
1409 1409 a, g dbSNP:775912118
1419 1419 c, t dbSNP:763506600
1420 1420 a, c dbSNP:764435229
1431 1431 c, t dbSNP:148828876
1432 1432 a, g dbSNP:373214858
1435 1435 a, g dbSNP:767175665
1447 1447 c, g dbSNP:750034699
1450 1450 a, c dbSNP:199857749
1451 1451 c, g dbSNP:376403172
1459 1459 a, g dbSNP:753776408
1462 1462 a, g dbSNP:201335041
1468 1468 -, a dbSNP:34709428
1468 1468 a, g dbSNP:778926078
1472 1472 a, g dbSNP:747979413
1481 1481 a, g dbSNP:771953345
1483 1483 c, t dbSNP:781607647
1486 1486 g, t dbSNP:746082440
1487 1487 c, t dbSNP:769825619
1488 1488 a, g dbSNP:143432552
1501 1501 a, c, g dbSNP:201777722
1504 1504 c, t dbSNP:2069849
1522 1522 a, g dbSNP:774788213
1528 1528 c, t dbSNP:762193118
1537 1537 a, g dbSNP:767834289
1543 1543 c, t dbSNP:111490870
1545 1545 g, t dbSNP:750089674
1555 1555 a, t dbSNP:760361679
1556 1556 -, ttg dbSNP:760390706
1562 1562 c, t dbSNP:765869669
1567 1567 a, g dbSNP:201868479
1571 1571 a, g dbSNP:200852701
1577 1577 c, t dbSNP:2069850
1578 1578 c, t dbSNP:368219445
1579 1579 a, c, t dbSNP:779055090
1585 1585 c, t dbSNP:199619831
1587 1587 a, g dbSNP:41477054
1590 1590 a, t dbSNP:201107891
1603 1603 c, t dbSNP:202198528
1607 1607 c, g dbSNP:200226108
1629 1629 c, t dbSNP:201743553
1632 1632 a, g dbSNP:199652344
1644 1644 a, g dbSNP:13306436
1651 1651 c, t dbSNP:201150447
1652 1652 a, g dbSNP:2069851
1675 1675 g, t dbSNP:200697926
1677 1677 a, t dbSNP:778912423
1699 1699 a, g dbSNP:201769340
1734 1734 a, g dbSNP:199606490
1752 1752 g, t dbSNP:55773150
1766 1766 c, g dbSNP:202045132
1790 1790 a, c dbSNP:200126515
1813 1813 c, t dbSNP:758388914
1824 1824 a, g dbSNP:201131164
1876 1876 c, t dbSNP:201722624
1880 1880 c, t dbSNP:199889619
1900 1900 c, t dbSNP:201176371
1904 1904 c, t dbSNP:202085240
1916 1916 a, g dbSNP:529245167
1920 1920 c, t dbSNP:183160679

Target ORF information:

RefSeq Version XM_011515390
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens interleukin 6 (IL6), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011515390

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGAACTCCT TCTCCACAAG CGCCTTCGGT CCAGTTGCCT TCTCCCTGGG GCTGCTCCTG 
GTGTTGCCTG CTGCCTTCCC TGCCCCAGTA CCCCCAGGAG AAGATTCCAA AGATGTAGCC
GCCCCACACA GACAGCCACT CACCTCTTCA GAACGAATTG ACAAACAAAT TCGGTACATC
CTCGACGGCA TCTCAGCCCT GAGAAAGGAG ACATGTAACA AGAGTAACAT GTGTGAAAGC
AGCAAAGAGG CACTGGCAGA AAACAACCTG AACCTTCCAA AGATGGCTGA AAAAGATGGA
TGCTTCCAAT CTGGATTCAA TGAGGAGACT TGCCTGGTGA AAATCATCAC TGGTCTTTTG
GAGTTTGAGG TATACCTAGA GTACCTCCAG AACAGATTTG AGAGTAGTGA GGAACAAGCC
AGAGCTGTGC AGATGAGTAC AAAAGTCCTG ATCCAGTTCC TGCAGAAAAA GGCAAAGAAT
CTAGATGCAA TAACCACCCC TGACCCAACC ACAAATGCCA GCCTGCTGAC GAAGCTGCAG
GCACAGAACC AGTGGCTGCA GGACATGACA ACTCATCTCA TTCTGCGCAG CTTTAAGGAG
TTCCTGCAGT CCAGCCTGAG GGCTCTTCGG CAAATGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58395
Accession Version XM_011515391.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 411bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product interleukin-6 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007819.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
ATGTGTGAAA GCAGCAAAGA GGCACTGGCA GAAAACAACC TGAACCTTCC AAAGATGGCT 
GAAAAAGATG GATGCTTCCA ATCTGGATTC AATGAGGAGA CTTGCCTGGT GAAAATCATC
ACTGGTCTTT TGGAGTTTGA GGTATACCTA GAGTACCTCC AGAACAGATT TGAGAGTAGT
GAGGAACAAG CCAGAGCTGT GCAGATGAGT ACAAAAGTCC TGATCCAGTT CCTGCAGAAA
AAGGCAAAGA ATCTAGATGC AATAACCACC CCTGACCCAA CCACAAATGC CAGCCTGCTG
ACGAAGCTGC AGGCACAGAA CCAGTGGCTG CAGGACATGA CAACTCATCT CATTCTGCGC
AGCTTTAAGG AGTTCCTGCA GTCCAGCCTG AGGGCTCTTC GGCAAATGTA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011513693.1
CDS139..549
Misc Feature(1)139..540(+)
Translation
Position Chain Variation Link
2 2 a, c dbSNP:201575115
26 26 a, g dbSNP:779901687
46 46 c, t dbSNP:199671830
52 52 g, t dbSNP:13306438
54 54 a, c dbSNP:2069857
56 56 a, g dbSNP:758839425
58 58 a, c dbSNP:185230192
59 59 a, g dbSNP:199546667
59 59 -, g dbSNP:778200235
63 63 a, g dbSNP:777978147
65 65 a, c, g dbSNP:746737342
69 69 c, g dbSNP:3087226
79 79 a, g dbSNP:368772259
80 80 c, t dbSNP:745397994
81 81 c, t dbSNP:769242976
90 90 a, c dbSNP:775434886
101 101 -, t dbSNP:745672376
106 106 a, t dbSNP:200700194
107 107 c, t dbSNP:768540611
110 110 c, t dbSNP:200815144
117 117 a, g dbSNP:569143621
118 118 c, t dbSNP:774003579
123 123 a, g, t dbSNP:145242107
124 124 c, t dbSNP:370818995
126 126 c, t dbSNP:147625275
131 131 a, g dbSNP:746269216
138 138 c, t dbSNP:770018245
139 139 a, g dbSNP:201121373
140 140 c, t dbSNP:775325818
145 145 c, g dbSNP:190436077
147 147 a, g dbSNP:200987147
150 150 c, t dbSNP:201523057
159 159 a, c, g, t dbSNP:142164099
160 160 a, g dbSNP:761157550
168 168 a, c dbSNP:375470014
170 170 a, g dbSNP:199990564
177 177 c, t dbSNP:750278000
181 181 a, t dbSNP:755968353
182 182 a, t dbSNP:200745306
183 183 c, t dbSNP:766129888
195 195 c, g dbSNP:752964616
205 205 c, g dbSNP:758616001
210 210 a, t dbSNP:777906302
218 218 a, t dbSNP:747126003
219 219 a, g dbSNP:757236211
221 221 a, c dbSNP:202210223
230 230 a, g dbSNP:781740062
236 236 a, g dbSNP:200263800
237 237 a, g dbSNP:751000694
244 244 c, g dbSNP:756681741
246 246 a, g dbSNP:780638841
256 256 a, t dbSNP:148171375
265 265 c, g dbSNP:769089379
266 266 c, t dbSNP:11544633
269 269 c, t dbSNP:778738185
280 280 a, g dbSNP:371302236
281 281 c, t dbSNP:771660418
291 291 g, t dbSNP:772582867
293 293 a, g dbSNP:201371019
300 300 c, g dbSNP:760165722
302 302 a, g dbSNP:770868572
308 308 a, t dbSNP:776504834
312 312 a, g dbSNP:759359627
316 316 a, g dbSNP:56383910
321 321 c, g dbSNP:764988588
332 332 a, g, t dbSNP:751859032
335 335 a, c dbSNP:767699802
356 356 a, t dbSNP:750469507
362 362 -, c dbSNP:34012176
366 366 c, g dbSNP:182812860
369 369 c, t dbSNP:199569510
374 374 a, g dbSNP:780726676
387 387 c, g, t dbSNP:200477775
392 392 c, t dbSNP:369953112
395 395 a, t dbSNP:2069860
396 396 a, t dbSNP:13306435
397 397 c, g dbSNP:768383637
398 398 c, t dbSNP:778836162
406 406 a, c dbSNP:747302620
407 407 c, t dbSNP:771282862
408 408 c, t dbSNP:541464782
409 409 c, g dbSNP:200641450
414 414 a, c, g dbSNP:201947383
415 415 c, t dbSNP:769888477
418 418 a, g dbSNP:775912118
428 428 c, t dbSNP:763506600
429 429 a, c dbSNP:764435229
440 440 c, t dbSNP:148828876
441 441 a, g dbSNP:373214858
444 444 a, g dbSNP:767175665
456 456 c, g dbSNP:750034699
459 459 a, c dbSNP:199857749
460 460 c, g dbSNP:376403172
468 468 a, g dbSNP:753776408
471 471 a, g dbSNP:201335041
477 477 -, a dbSNP:34709428
477 477 a, g dbSNP:778926078
481 481 a, g dbSNP:747979413
490 490 a, g dbSNP:771953345
492 492 c, t dbSNP:781607647
495 495 g, t dbSNP:746082440
496 496 c, t dbSNP:769825619
497 497 a, g dbSNP:143432552
510 510 a, c, g dbSNP:201777722
513 513 c, t dbSNP:2069849
531 531 a, g dbSNP:774788213
537 537 c, t dbSNP:762193118
546 546 a, g dbSNP:767834289
552 552 c, t dbSNP:111490870
554 554 g, t dbSNP:750089674
564 564 a, t dbSNP:760361679
565 565 -, ttg dbSNP:760390706
571 571 c, t dbSNP:765869669
576 576 a, g dbSNP:201868479
580 580 a, g dbSNP:200852701
586 586 c, t dbSNP:2069850
587 587 c, t dbSNP:368219445
588 588 a, c, t dbSNP:779055090
594 594 c, t dbSNP:199619831
596 596 a, g dbSNP:41477054
599 599 a, t dbSNP:201107891
612 612 c, t dbSNP:202198528
616 616 c, g dbSNP:200226108
638 638 c, t dbSNP:201743553
641 641 a, g dbSNP:199652344
653 653 a, g dbSNP:13306436
660 660 c, t dbSNP:201150447
661 661 a, g dbSNP:2069851
684 684 g, t dbSNP:200697926
686 686 a, t dbSNP:778912423
708 708 a, g dbSNP:201769340
743 743 a, g dbSNP:199606490
761 761 g, t dbSNP:55773150
775 775 c, g dbSNP:202045132
799 799 a, c dbSNP:200126515
822 822 c, t dbSNP:758388914
833 833 a, g dbSNP:201131164
885 885 c, t dbSNP:201722624
889 889 c, t dbSNP:199889619
909 909 c, t dbSNP:201176371
913 913 c, t dbSNP:202085240
925 925 a, g dbSNP:529245167
929 929 c, t dbSNP:183160679

Target ORF information:

RefSeq Version XM_011515391
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens interleukin 6 (IL6), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011515391

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGTGTGAAA GCAGCAAAGA GGCACTGGCA GAAAACAACC TGAACCTTCC AAAGATGGCT 
GAAAAAGATG GATGCTTCCA ATCTGGATTC AATGAGGAGA CTTGCCTGGT GAAAATCATC
ACTGGTCTTT TGGAGTTTGA GGTATACCTA GAGTACCTCC AGAACAGATT TGAGAGTAGT
GAGGAACAAG CCAGAGCTGT GCAGATGAGT ACAAAAGTCC TGATCCAGTT CCTGCAGAAA
AAGGCAAAGA ATCTAGATGC AATAACCACC CCTGACCCAA CCACAAATGC CAGCCTGCTG
ACGAAGCTGC AGGCACAGAA CCAGTGGCTG CAGGACATGA CAACTCATCT CATTCTGCGC
AGCTTTAAGG AGTTCCTGCA GTCCAGCCTG AGGGCTCTTC GGCAAATGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23477
Accession Version NM_000600.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 639bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu23477D_COA.pdf (pdf)
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product interleukin-6 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK301141.1, BC015511.1 and AA721478.1. This sequence is a reference standard in the RefSeqGene project. On Mar 11, 2009 this sequence version replaced gi:155369258. Summary: This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X04430.1, BC015511.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGAACTCCT TCTCCACAAG CGCCTTCGGT CCAGTTGCCT TCTCCCTGGG GCTGCTCCTG 
GTGTTGCCTG CTGCCTTCCC TGCCCCAGTA CCCCCAGGAG AAGATTCCAA AGATGTAGCC
GCCCCACACA GACAGCCACT CACCTCTTCA GAACGAATTG ACAAACAAAT TCGGTACATC
CTCGACGGCA TCTCAGCCCT GAGAAAGGAG ACATGTAACA AGAGTAACAT GTGTGAAAGC
AGCAAAGAGG CACTGGCAGA AAACAACCTG AACCTTCCAA AGATGGCTGA AAAAGATGGA
TGCTTCCAAT CTGGATTCAA TGAGGAGACT TGCCTGGTGA AAATCATCAC TGGTCTTTTG
GAGTTTGAGG TATACCTAGA GTACCTCCAG AACAGATTTG AGAGTAGTGA GGAACAAGCC
AGAGCTGTGC AGATGAGTAC AAAAGTCCTG ATCCAGTTCC TGCAGAAAAA GGCAAAGAAT
CTAGATGCAA TAACCACCCC TGACCCAACC ACAAATGCCA GCCTGCTGAC GAAGCTGCAG
GCACAGAACC AGTGGCTGCA GGACATGACA ACTCATCTCA TTCTGCGCAG CTTTAAGGAG
TTCCTGCAGT CCAGCCTGAG GGCTCTTCGG CAAATGTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000591.1
CDS117..755
Misc Feature(1)30..32(+)
Misc Feature(2)285..746(+)
Misc Feature(3)330..350(+)
Misc Feature(4)333..335(+)
Misc Feature(5)357..359(+)
Misc Feature(6)417..449(+)
Misc Feature(7)612..614(+)
Misc Feature(8)624..626(+)
Misc Feature(9)627..629(+)
Exon (1)1..135
Gene:IL6
Gene Synonym:
Exon (2)136..326
Gene:IL6
Gene Synonym:
Exon (3)327..440
Gene:IL6
Gene Synonym:
Exon (4)441..587
Gene:IL6
Gene Synonym:
Exon (5)588..1184
Gene:IL6
Gene Synonym:
Translation
Position Chain Variation Link
17 17 a, c dbSNP:201575115
41 41 a, g dbSNP:779901687
61 61 c, t dbSNP:199671830
67 67 g, t dbSNP:13306438
69 69 a, c dbSNP:2069857
71 71 a, g dbSNP:758839425
73 73 a, c dbSNP:185230192
74 74 a, g dbSNP:199546667
74 74 -, g dbSNP:778200235
78 78 a, g dbSNP:777978147
80 80 a, c, g dbSNP:746737342
84 84 c, g dbSNP:3087226
94 94 a, g dbSNP:368772259
95 95 c, t dbSNP:745397994
96 96 c, t dbSNP:769242976
105 105 a, c dbSNP:775434886
116 116 -, t dbSNP:745672376
121 121 a, t dbSNP:200700194
122 122 c, t dbSNP:768540611
125 125 c, t dbSNP:200815144
132 132 a, g dbSNP:569143621
133 133 c, t dbSNP:774003579
137 137 a, c dbSNP:199941251
140 140 a, c dbSNP:200637969
145 145 c, g dbSNP:762283178
153 153 g, t dbSNP:767924065
154 154 c, t dbSNP:750778143
157 157 -, tc dbSNP:779919594
158 158 a, c dbSNP:760962452
159 159 -, c dbSNP:34280821
164 164 g, t dbSNP:766033770
168 168 c, t dbSNP:753479461
176 176 a, g dbSNP:201852608
180 180 c, t dbSNP:148516576
186 186 c, g dbSNP:746848366
207 207 a, c dbSNP:142759801
208 208 c, g dbSNP:778465778
210 210 c, t dbSNP:2069830
212 212 a, g dbSNP:758406611
220 220 a, t dbSNP:201787553
232 232 c, t dbSNP:769904935
237 237 a, g dbSNP:746818346
240 240 c, g dbSNP:770802865
244 244 a, g dbSNP:780174319
246 246 a, c dbSNP:199957016
251 251 g, t dbSNP:544833266
259 259 c, t dbSNP:768665469
270 270 a, c dbSNP:774472593
274 274 c, t dbSNP:762371056
279 279 a, g dbSNP:202138427
280 280 a, g dbSNP:541074925
284 284 a, c dbSNP:199925567
290 290 a, g dbSNP:200999878
291 291 c, t dbSNP:201822486
296 296 a, c dbSNP:753484746
299 299 c, t dbSNP:140764737
302 302 c, t dbSNP:764720869
303 303 a, g, t dbSNP:752257060
311 311 a, c dbSNP:201410817
329 329 a, g, t dbSNP:145242107
330 330 c, t dbSNP:370818995
332 332 c, t dbSNP:147625275
337 337 a, g dbSNP:746269216
344 344 c, t dbSNP:770018245
345 345 a, g dbSNP:201121373
346 346 c, t dbSNP:775325818
351 351 c, g dbSNP:190436077
353 353 a, g dbSNP:200987147
356 356 c, t dbSNP:201523057
365 365 a, c, g, t dbSNP:142164099
366 366 a, g dbSNP:761157550
374 374 a, c dbSNP:375470014
376 376 a, g dbSNP:199990564
383 383 c, t dbSNP:750278000
387 387 a, t dbSNP:755968353
388 388 a, t dbSNP:200745306
389 389 c, t dbSNP:766129888
401 401 c, g dbSNP:752964616
411 411 c, g dbSNP:758616001
416 416 a, t dbSNP:777906302
424 424 a, t dbSNP:747126003
425 425 a, g dbSNP:757236211
427 427 a, c dbSNP:202210223
436 436 a, g dbSNP:781740062
442 442 a, g dbSNP:200263800
443 443 a, g dbSNP:751000694
450 450 c, g dbSNP:756681741
452 452 a, g dbSNP:780638841
462 462 a, t dbSNP:148171375
471 471 c, g dbSNP:769089379
472 472 c, t dbSNP:11544633
475 475 c, t dbSNP:778738185
486 486 a, g dbSNP:371302236
487 487 c, t dbSNP:771660418
497 497 g, t dbSNP:772582867
499 499 a, g dbSNP:201371019
506 506 c, g dbSNP:760165722
508 508 a, g dbSNP:770868572
514 514 a, t dbSNP:776504834
518 518 a, g dbSNP:759359627
522 522 a, g dbSNP:56383910
527 527 c, g dbSNP:764988588
538 538 a, g, t dbSNP:751859032
541 541 a, c dbSNP:767699802
562 562 a, t dbSNP:750469507
568 568 -, c dbSNP:34012176
572 572 c, g dbSNP:182812860
575 575 c, t dbSNP:199569510
580 580 a, g dbSNP:780726676
593 593 c, g, t dbSNP:200477775
598 598 c, t dbSNP:369953112
601 601 a, t dbSNP:2069860
602 602 a, t dbSNP:13306435
603 603 c, g dbSNP:768383637
604 604 c, t dbSNP:778836162
612 612 a, c dbSNP:747302620
613 613 c, t dbSNP:771282862
614 614 c, t dbSNP:541464782
615 615 c, g dbSNP:200641450
620 620 a, c, g dbSNP:201947383
621 621 c, t dbSNP:769888477
624 624 a, g dbSNP:775912118
634 634 c, t dbSNP:763506600
635 635 a, c dbSNP:764435229
646 646 c, t dbSNP:148828876
647 647 a, g dbSNP:373214858
650 650 a, g dbSNP:767175665
662 662 c, g dbSNP:750034699
665 665 a, c dbSNP:199857749
666 666 c, g dbSNP:376403172
674 674 a, g dbSNP:753776408
677 677 a, g dbSNP:201335041
683 683 -, a dbSNP:34709428
683 683 a, g dbSNP:778926078
687 687 a, g dbSNP:747979413
696 696 a, g dbSNP:771953345
698 698 c, t dbSNP:781607647
701 701 g, t dbSNP:746082440
702 702 c, t dbSNP:769825619
703 703 a, g dbSNP:143432552
716 716 a, c, g dbSNP:201777722
719 719 c, t dbSNP:2069849
737 737 a, g dbSNP:774788213
743 743 c, t dbSNP:762193118
752 752 a, g dbSNP:767834289
758 758 c, t dbSNP:111490870
760 760 g, t dbSNP:750089674
770 770 a, t dbSNP:760361679
771 771 -, ttg dbSNP:760390706
777 777 c, t dbSNP:765869669
782 782 a, g dbSNP:201868479
786 786 a, g dbSNP:200852701
792 792 c, t dbSNP:2069850
793 793 c, t dbSNP:368219445
794 794 a, c, t dbSNP:779055090
800 800 c, t dbSNP:199619831
802 802 a, g dbSNP:41477054
805 805 a, t dbSNP:201107891
818 818 c, t dbSNP:202198528
822 822 c, g dbSNP:200226108
844 844 c, t dbSNP:201743553
847 847 a, g dbSNP:199652344
859 859 a, g dbSNP:13306436
866 866 c, t dbSNP:201150447
867 867 a, g dbSNP:2069851
890 890 g, t dbSNP:200697926
892 892 a, t dbSNP:778912423
914 914 a, g dbSNP:201769340
949 949 a, g dbSNP:199606490
967 967 g, t dbSNP:55773150
981 981 c, g dbSNP:202045132
1005 1005 a, c dbSNP:200126515
1028 1028 c, t dbSNP:758388914
1039 1039 a, g dbSNP:201131164
1091 1091 c, t dbSNP:201722624
1095 1095 c, t dbSNP:199889619
1115 1115 c, t dbSNP:201176371
1119 1119 c, t dbSNP:202085240
1131 1131 a, g dbSNP:529245167
1135 1135 c, t dbSNP:183160679

Target ORF information:

RefSeq Version NM_000600
Organism Homo sapiens (human)
Definition Homo sapiens interleukin 6 (IL6), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000600

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGAACTCCT TCTCCACAAG CGCCTTCGGT CCAGTTGCCT TCTCCCTGGG GCTGCTCCTG 
GTGTTGCCTG CTGCCTTCCC TGCCCCAGTA CCCCCAGGAG AAGATTCCAA AGATGTAGCC
GCCCCACACA GACAGCCACT CACCTCTTCA GAACGAATTG ACAAACAAAT TCGGTACATC
CTCGACGGCA TCTCAGCCCT GAGAAAGGAG ACATGTAACA AGAGTAACAT GTGTGAAAGC
AGCAAAGAGG CACTGGCAGA AAACAACCTG AACCTTCCAA AGATGGCTGA AAAAGATGGA
TGCTTCCAAT CTGGATTCAA TGAGGAGACT TGCCTGGTGA AAATCATCAC TGGTCTTTTG
GAGTTTGAGG TATACCTAGA GTACCTCCAG AACAGATTTG AGAGTAGTGA GGAACAAGCC
AGAGCTGTGC AGATGAGTAC AAAAGTCCTG ATCCAGTTCC TGCAGAAAAA GGCAAAGAAT
CTAGATGCAA TAACCACCCC TGACCCAACC ACAAATGCCA GCCTGCTGAC GAAGCTGCAG
GCACAGAACC AGTGGCTGCA GGACATGACA ACTCATCTCA TTCTGCGCAG CTTTAAGGAG
TTCCTGCAGT CCAGCCTGAG GGCTCTTCGG CAAATGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.