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CXCL8 cDNA ORF clone, Homo sapiens (human)

Gene Symbol CXCL8
Entrez Gene ID 3576
Full Name chemokine (C-X-C motif) ligand 8
Synonyms GCP-1, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1
General protein information
Preferred Names
interleukin-8
Names
interleukin-8
emoctakin
T-cell chemotactic factor
neutrophil-activating peptide 1
chemokine (C-X-C motif) ligand 8
beta-thromboglobulin-like protein
granulocyte chemotactic protein 1
tumor necrosis factor-induced gene 1
alveolar macrophage chemotactic factor I
monocyte-derived neutrophil chemotactic factor
monocyte-derived neutrophil-activating peptide
small inducible cytokine subfamily B, member 8
lymphocyte derived neutrophil activating peptide
lung giant cell carcinoma-derived chemotactic protein
beta endothelial cell-derived neutrophil activating peptide
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

4

4q13-q21

Summary The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

146930


mRNA and Protein(s)

mRNA Protein Name
NM_000584 NP_000575 interleukin-8 precursor



Homo sapiens (human) IL8 NP_000575.1
Pan troglodytes (chimpanzee) IL8 XP_001156432.1
Macaca mulatta (Rhesus monkey) IL8 NP_001028137.1
Canis lupus familiaris (dog) IL8 NP_001003200.1
Bos taurus (cattle) IL8 NP_776350.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CXCL8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CXCL8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu20145
NM_000584 Homo sapiens chemokine (C-X-C motif) ligand 8 (CXCL8), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20145
Clone ID Related Accession (Same CDS sequence) NM_000584
Accession Version NM_000584.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 300bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product interleukin-8 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG497712.1 and BC013615.1. This sequence is a reference standard in the RefSeqGene project. On Feb 25, 2011 this sequence version replaced gi:28610153. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M26383.1, AK131067.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
ATGACTTCCA AGCTGGCCGT GGCTCTCTTG GCAGCCTTCC TGATTTCTGC AGCTCTGTGT 
GAAGGTGCAG TTTTGCCAAG GAGTGCTAAA GAACTTAGAT GTCAGTGCAT AAAGACATAC
TCCAAACCTT TCCACCCCAA ATTTATCAAA GAACTGAGAG TGATTGAGAG TGGACCACAC
TGCGCCAACA CAGAAATTAT TGTAAAGCTT TCTGATGGAA GAGAGCTCTG TCTGGACCCC
AAGGAAAACT GGGTGCAGAG GGTTGTGGAG AAGTTTTTGA AGAGGGCTGA GAATTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000575.1
CDS154..453
Misc Feature(1)58..60(+)
Misc Feature(2)232..237(+)
Misc Feature(3)232..234(+)
Misc Feature(4)232..234(+)
Misc Feature(5)235..240(+)
Misc Feature(6)235..237(+)
Misc Feature(7)244..435(+)
Misc Feature(8)244..414(+)
Misc Feature(9)244..252(+)
Misc Feature(10)247..432(+)
Misc Feature(11)247..378(+)
Misc Feature(12)250..261(+)
Misc Feature(13)256..300(+)
Misc Feature(14)262..381(+)
Misc Feature(15)286..435(+)
Misc Feature(16)292..378(+)
Misc Feature(17)298..432(+)
Misc Feature(18)325..333(+)
Misc Feature(19)325..333(+)
Misc Feature(20)364..372(+)
Exon (1)1..217
Gene:CXCL8
Gene Synonym:
Exon (2)218..353
Gene:CXCL8
Gene Synonym:
Exon (3)354..437
Gene:CXCL8
Gene Synonym:
Exon (4)438..1705
Gene:CXCL8
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000584
Organism Homo sapiens (human)
Definition Homo sapiens chemokine (C-X-C motif) ligand 8 (CXCL8), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000584

ORF Insert Sequence:

1
61
121
181
241
ATGACTTCCA AGCTGGCCGT GGCTCTCTTG GCAGCCTTCC TGATTTCTGC AGCTCTGTGT 
GAAGGTGCAG TTTTGCCAAG GAGTGCTAAA GAACTTAGAT GTCAGTGCAT AAAGACATAC
TCCAAACCTT TCCACCCCAA ATTTATCAAA GAACTGAGAG TGATTGAGAG TGGACCACAC
TGCGCCAACA CAGAAATTAT TGTAAAGCTT TCTGATGGAA GAGAGCTCTG TCTGGACCCC
AAGGAAAACT GGGTGCAGAG GGTTGTGGAG AAGTTTTTGA AGAGGGCTGA GAATTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

TSG-6 inhibits neutrophil migration via direct interaction with the chemokine CXCL8
J. Immunol. 192 (5), 2177-2185 (2014)
Dyer DP, Thomson JM, Hermant A, Jowitt TA, Handel TM, Proudfoot AE, Day AJ and Milner CM.


book

Dynamics of chemokine, cytokine, and growth factor serum levels in BRAF-mutant melanoma patients during BRAF inhibitor treatment
J. Immunol. 192 (5), 2505-2513 (2014)
Wilmott JS, Haydu LE, Menzies AM, Lum T, Hyman J, Thompson JF, Hersey P, Kefford RF, Scolyer RA and Long GV.


book

Proteasome inhibition increases recruitment of IkappaB kinase beta (IKKbeta), S536P-p65, and transcription factor EGR1 to interleukin-8 (IL-8) promoter, resulting in increased IL-8 production in ovarian cancer cells
J. Biol. Chem. 289 (5), 2687-2700 (2014)
Singha B, Gatla HR, Manna S, Chang TP, Sanacora S, Poltoratsky V, Vancura A and Vancurova I.


book

Blood eosinophil and platelet levels, proteomics patterns of trail and CXCL8 correlated with survival in bevacizumab treated metastatic colon cancers
Clin. Lab. 60 (2), 339-340 (2014)
Yalcin,A.D., Kargi,A., Gumuslu,S. and Strauss,L.G.


book

The prognostic role of interleukin-8 (IL-8) and matrix metalloproteinases -2 and -9 in lymph node-negative untreated breast cancer patients
J BUON 18 (4), 866-873 (2013)
Milovanovic J, Todorovic-Rakovic N and Abu Rabi Z.


book

Interleukin-8 as a macrophage-derived mediator of angiogenesis
Science 258 (5089), 1798-1801 (1992)
Koch AE, Polverini PJ, Kunkel SL, Harlow LA, DiPietro LA, Elner VM, Elner SG and Strieter RM.


book

Human placenta constitutively produces interleukin-8 during pregnancy and enhances its production in intrauterine infection
Biol. Reprod. 47 (2), 220-226 (1992)
Shimoya K, Matsuzaki N, Taniguchi T, Kameda T, Koyama M, Neki R, Saji F and Tanizawa O.


book

Interleukin-8, a chemotactic and inflammatory cytokine
FEBS Lett. 307 (1), 97-101 (1992)
Baggiolini M and Clark-Lewis I.


book

Structure and functional expression of a human interleukin-8 receptor
Science 253 (5025), 1278-1280 (1991)
Holmes WE, Lee J, Kuang WJ, Rice GC and Wood WI.


book

Selective attraction of monocytes and T lymphocytes of the memory phenotype by cytokine RANTES
Nature 347 (6294), 669-671 (1990)
Schall TJ, Bacon K, Toy KJ and Goeddel DV.


 
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