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IL12B cDNA ORF clone, Homo sapiens (human)

Gene Symbol IL12B
Entrez Gene ID 3593
Full Name interleukin 12B
Synonyms CLMF, CLMF2, IL-12B, IMD28, IMD29, NKSF, NKSF2
General protein information
Preferred Names
interleukin-12 subunit beta
Names
interleukin-12 subunit beta
CLMF p40
IL-12 subunit p40
IL12, subunit p40
interleukin 12, p40
interleukin-12 beta chain
NK cell stimulatory factor chain 2
cytotoxic lymphocyte maturation factor 40 kDa subunit
natural killer cell stimulatory factor, 40 kD subunit
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

5

5q33.3

Summary This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

161561

Disorder Html: BCG and salmonella infection, disseminated, 209950 (1); {Asthma,

mRNA and Protein(s)

mRNA Protein Name
NM_002187 NP_002178 interleukin-12 subunit beta precursor



Homo sapiens (human) IL12B NP_002178.2
Pan troglodytes (chimpanzee) IL12B XP_527101.2
Macaca mulatta (Rhesus monkey) IL12B NP_001038190.1
Canis lupus familiaris (dog) IL12B NP_001003292.1
Bos taurus (cattle) IL12B NP_776781.1
Mus musculus (house mouse) Il12b NP_032378.1
Rattus norvegicus (Norway rat) Il12b NP_072133.1
Gallus gallus (chicken) IL12B NP_998736.1
Danio rerio (zebrafish) il12ba NP_001007109.1
Danio rerio (zebrafish) il12bb XP_002666092.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following IL12B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IL12B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu20878
NM_002187 Homo sapiens interleukin 12B (IL12B), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$154.50-$216.30
$309.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20878
Clone ID Related Accession (Same CDS sequence) NM_002187
Accession Version NM_002187.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 987bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 10-JUL-2015
Organism Homo sapiens (human)
Product interleukin-12 subunit beta precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M65272.1, M65290.1 and AC011418.5. On Nov 3, 2002 this sequence version replaced gi:4504640. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M65290.1, BC074723.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2152719 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTGTCACC AGCAGTTGGT CATCTCTTGG TTTTCCCTGG TTTTTCTGGC ATCTCCCCTC 
GTGGCCATAT GGGAACTGAA GAAAGATGTT TATGTCGTAG AATTGGATTG GTATCCGGAT
GCCCCTGGAG AAATGGTGGT CCTCACCTGT GACACCCCTG AAGAAGATGG TATCACCTGG
ACCTTGGACC AGAGCAGTGA GGTCTTAGGC TCTGGCAAAA CCCTGACCAT CCAAGTCAAA
GAGTTTGGAG ATGCTGGCCA GTACACCTGT CACAAAGGAG GCGAGGTTCT AAGCCATTCG
CTCCTGCTGC TTCACAAAAA GGAAGATGGA ATTTGGTCCA CTGATATTTT AAAGGACCAG
AAAGAACCCA AAAATAAGAC CTTTCTAAGA TGCGAGGCCA AGAATTATTC TGGACGTTTC
ACCTGCTGGT GGCTGACGAC AATCAGTACT GATTTGACAT TCAGTGTCAA AAGCAGCAGA
GGCTCTTCTG ACCCCCAAGG GGTGACGTGC GGAGCTGCTA CACTCTCTGC AGAGAGAGTC
AGAGGGGACA ACAAGGAGTA TGAGTACTCA GTGGAGTGCC AGGAGGACAG TGCCTGCCCA
GCTGCTGAGG AGAGTCTGCC CATTGAGGTC ATGGTGGATG CCGTTCACAA GCTCAAGTAT
GAAAACTACA CCAGCAGCTT CTTCATCAGG GACATCATCA AACCTGACCC ACCCAAGAAC
TTGCAGCTGA AGCCATTAAA GAATTCTCGG CAGGTGGAGG TCAGCTGGGA GTACCCTGAC
ACCTGGAGTA CTCCACATTC CTACTTCTCC CTGACATTCT GCGTTCAGGT CCAGGGCAAG
AGCAAGAGAG AAAAGAAAGA TAGAGTCTTC ACGGACAAGA CCTCAGCCAC GGTCATCTGC
CGCAAAAATG CCAGCATTAG CGTGCGGGCC CAGGACCGCT ACTATAGCTC ATCTTGGAGC
GAATGGGCAT CTGTGCCCTG CAGTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002178.2
CDS43..1029
Misc Feature(1)166..312(+)
Misc Feature(2)415..693(+)
Misc Feature(3)745..1017(+)
Misc Feature(4)745..987(+)
Misc Feature(5)988..1002(+)
Exon (1)1..42
Gene:IL12B
Gene Synonym:
Exon (2)43..130
Gene:IL12B
Gene Synonym:
Exon (3)131..406
Gene:IL12B
Gene Synonym:
Exon (4)407..524
Gene:IL12B
Gene Synonym:
Exon (5)525..739
Gene:IL12B
Gene Synonym:
Exon (6)740..897
Gene:IL12B
Gene Synonym:
Exon (7)898..1029
Gene:IL12B
Gene Synonym:
Exon (8)1030..2347
Gene:IL12B
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002187
Organism Homo sapiens (human)
Definition Homo sapiens interleukin 12B (IL12B), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002187

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTGTCACC AGCAGTTGGT CATCTCTTGG TTTTCCCTGG TTTTTCTGGC ATCTCCCCTC 
GTGGCCATAT GGGAACTGAA GAAAGATGTT TATGTCGTAG AATTGGATTG GTATCCGGAT
GCCCCTGGAG AAATGGTGGT CCTCACCTGT GACACCCCTG AAGAAGATGG TATCACCTGG
ACCTTGGACC AGAGCAGTGA GGTCTTAGGC TCTGGCAAAA CCCTGACCAT CCAAGTCAAA
GAGTTTGGAG ATGCTGGCCA GTACACCTGT CACAAAGGAG GCGAGGTTCT AAGCCATTCG
CTCCTGCTGC TTCACAAAAA GGAAGATGGA ATTTGGTCCA CTGATATTTT AAAGGACCAG
AAAGAACCCA AAAATAAGAC CTTTCTAAGA TGCGAGGCCA AGAATTATTC TGGACGTTTC
ACCTGCTGGT GGCTGACGAC AATCAGTACT GATTTGACAT TCAGTGTCAA AAGCAGCAGA
GGCTCTTCTG ACCCCCAAGG GGTGACGTGC GGAGCTGCTA CACTCTCTGC AGAGAGAGTC
AGAGGGGACA ACAAGGAGTA TGAGTACTCA GTGGAGTGCC AGGAGGACAG TGCCTGCCCA
GCTGCTGAGG AGAGTCTGCC CATTGAGGTC ATGGTGGATG CCGTTCACAA GCTCAAGTAT
GAAAACTACA CCAGCAGCTT CTTCATCAGG GACATCATCA AACCTGACCC ACCCAAGAAC
TTGCAGCTGA AGCCATTAAA GAATTCTCGG CAGGTGGAGG TCAGCTGGGA GTACCCTGAC
ACCTGGAGTA CTCCACATTC CTACTTCTCC CTGACATTCT GCGTTCAGGT CCAGGGCAAG
AGCAAGAGAG AAAAGAAAGA TAGAGTCTTC ACGGACAAGA CCTCAGCCAC GGTCATCTGC
CGCAAAAATG CCAGCATTAG CGTGCGGGCC CAGGACCGCT ACTATAGCTC ATCTTGGAGC
GAATGGGCAT CTGTGCCCTG CAGTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Association of polymorphisms in interleukin (IL)-12A and -B genes with rheumatoid arthritis in a Chinese population
Clin. Exp. Immunol. 180 (1), 83-89 (2015)
Wang EY, Yang Q and Liao ZG.


book

IL12p40 regulates functional development of human CD4+ T cells: enlightenment by the elevated expressions of IL12p40 in patients with inflammatory bowel diseases
Medicine (Baltimore) 94 (10), E613 (2015)
Wang X, Wu T, Zhou F, Liu S, Zhou R, Zhu S, Song L, Zhu F, Wang G and Xia B.


book

Discovery of six new susceptibility loci and analysis of pleiotropic effects in leprosy
Nat. Genet. 47 (3), 267-271 (2015)
Liu H, Irwanto A, Fu X, Yu G, Yu Y, Sun Y, Wang C, Wang Z, Okada Y, Low H, Li Y, Liany H, Chen M, Bao F, Li J, You J, Zhang Q, Liu J, Chu T, Andiappan AK, Wang N, Niu G, Liu D, Yu X, Zhang L, Tian H, Zhou G, Rotzschke O, Chen S, Zhang X, Liu J and Zhang F.


book

An essential role of syntaxin 3 protein for granule exocytosis and secretion of IL-1alpha, IL-1beta, IL-12b, and CCL4 from differentiated HL-60 cells
J. Leukoc. Biol. 97 (3), 557-571 (2015)
Naegelen I, Plancon S, Nicot N, Kaoma T, Muller A, Vallar L, Tschirhart EJ and Brechard S.


book

Serum levels of TNF- alpha, IL-12/23 p40, and IL-17 in psoriatic patients with and without nail psoriasis: a cross-sectional study
ScientificWorldJournal 2014, 508178 (2014)
Kyriakou A, Patsatsi A, Vyzantiadis TA and Sotiriadis D.


book

Assignment of genes encoding a unique cytokine (IL12) composed of two unrelated subunits to chromosomes 3 and 5
Genomics 14 (1), 59-62 (1992)
Sieburth D, Jabs EW, Warrington JA, Li X, Lasota J, LaForgia S, Kelleher K, Huebner K, Wasmuth JJ and Wolf SF.


book

Homology of the p40 subunit of natural killer cell stimulatory factor (NKSF) with the extracellular domain of the interleukin-6 receptor
Cell 66 (1), 9-10 (1991)
Gearing,D.P. and Cosman,D.


book

Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor
Proc. Natl. Acad. Sci. U.S.A. 88 (10), 4143-4147 (1991)
Gubler U, Chua AO, Schoenhaut DS, Dwyer CM, McComas W, Motyka R, Nabavi N, Wolitzky AG, Quinn PM, Familletti PC et al.


book

Cloning of cDNA for natural killer cell stimulatory factor, a heterodimeric cytokine with multiple biologic effects on T and natural killer cells
J. Immunol. 146 (9), 3074-3081 (1991)
Wolf SF, Temple PA, Kobayashi M, Young D, Dicig M, Lowe L, Dzialo R, Fitz L, Ferenz C, Hewick RM et al.


book

Purification to homogeneity and partial characterization of cytotoxic lymphocyte maturation factor from human B-lymphoblastoid cells
Proc. Natl. Acad. Sci. U.S.A. 87 (17), 6808-6812 (1990)
Stern AS, Podlaski FJ, Hulmes JD, Pan YC, Quinn PM, Wolitzky AG, Familletti PC, Stremlo DL, Truitt T, Chizzonite R et al.


 
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