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IL12B interleukin 12B [Homo sapiens (human)]

Gene Symbol IL12B
Entrez Gene ID 3593
Full Name interleukin 12B
Synonyms CLMF, CLMF2, IL-12B, IMD28, IMD29, NKSF, NKSF2
General protein information
Preferred Names
interleukin-12 subunit beta
Names
interleukin-12 subunit beta
CLMF p40
IL-12 subunit p40
IL12, subunit p40
interleukin 12, p40
interleukin-12 beta chain
NK cell stimulatory factor chain 2
cytotoxic lymphocyte maturation factor 40 kDa subunit
natural killer cell stimulatory factor, 40 kD subunit
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

5

5q33.3

Summary This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

161561

Disorder Html: BCG and salmonella infection, disseminated, 209950 (1); {Asthma,





The following IL12B gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IL12B gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu20878 NM_002187 Homo sapiens interleukin 12B (IL12B), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$309

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu20878
Accession Version NM_002187.2
Sequence Information ORF Nucleotide Sequence (Length: 987bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 10-JUL-2015
Organism Homo sapiens (human)
Product interleukin-12 subunit beta precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M65272.1, M65290.1 and AC011418.5. On Nov 3, 2002 this sequence version replaced gi:4504640. Summary: This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. [provided by RefSeq, Jul 2008]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M65290.1, BC074723.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2152719 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTGTCACC AGCAGTTGGT CATCTCTTGG TTTTCCCTGG TTTTTCTGGC ATCTCCCCTC 
GTGGCCATAT GGGAACTGAA GAAAGATGTT TATGTCGTAG AATTGGATTG GTATCCGGAT
GCCCCTGGAG AAATGGTGGT CCTCACCTGT GACACCCCTG AAGAAGATGG TATCACCTGG
ACCTTGGACC AGAGCAGTGA GGTCTTAGGC TCTGGCAAAA CCCTGACCAT CCAAGTCAAA
GAGTTTGGAG ATGCTGGCCA GTACACCTGT CACAAAGGAG GCGAGGTTCT AAGCCATTCG
CTCCTGCTGC TTCACAAAAA GGAAGATGGA ATTTGGTCCA CTGATATTTT AAAGGACCAG
AAAGAACCCA AAAATAAGAC CTTTCTAAGA TGCGAGGCCA AGAATTATTC TGGACGTTTC
ACCTGCTGGT GGCTGACGAC AATCAGTACT GATTTGACAT TCAGTGTCAA AAGCAGCAGA
GGCTCTTCTG ACCCCCAAGG GGTGACGTGC GGAGCTGCTA CACTCTCTGC AGAGAGAGTC
AGAGGGGACA ACAAGGAGTA TGAGTACTCA GTGGAGTGCC AGGAGGACAG TGCCTGCCCA
GCTGCTGAGG AGAGTCTGCC CATTGAGGTC ATGGTGGATG CCGTTCACAA GCTCAAGTAT
GAAAACTACA CCAGCAGCTT CTTCATCAGG GACATCATCA AACCTGACCC ACCCAAGAAC
TTGCAGCTGA AGCCATTAAA GAATTCTCGG CAGGTGGAGG TCAGCTGGGA GTACCCTGAC
ACCTGGAGTA CTCCACATTC CTACTTCTCC CTGACATTCT GCGTTCAGGT CCAGGGCAAG
AGCAAGAGAG AAAAGAAAGA TAGAGTCTTC ACGGACAAGA CCTCAGCCAC GGTCATCTGC
CGCAAAAATG CCAGCATTAG CGTGCGGGCC CAGGACCGCT ACTATAGCTC ATCTTGGAGC
GAATGGGCAT CTGTGCCCTG CAGTTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_002178.2
CDS43..1029
Misc Feature(1)166..312(+)
Misc Feature(2)415..693(+)
Misc Feature(3)745..1017(+)
Misc Feature(4)745..987(+)
Misc Feature(5)988..1002(+)
Exon (1)1..42
Gene:IL12B
Gene Synonym:
Exon (2)43..130
Gene:IL12B
Gene Synonym:
Exon (3)131..406
Gene:IL12B
Gene Synonym:
Exon (4)407..524
Gene:IL12B
Gene Synonym:
Exon (5)525..739
Gene:IL12B
Gene Synonym:
Exon (6)740..897
Gene:IL12B
Gene Synonym:
Exon (7)898..1029
Gene:IL12B
Gene Synonym:
Exon (8)1030..2347
Gene:IL12B
Gene Synonym:
Translation
Position Chain Variation Link
57 57 c, g dbSNP:749205262
65 65 c, t dbSNP:372473387
66 66 c, g, t dbSNP:769479312
72 72 a, c, g dbSNP:763190982
74 74 c, t dbSNP:754651345
78 78 c, g dbSNP:139890370
82 82 g, t dbSNP:779736965
102 102 c, t dbSNP:757871778
103 103 a, g dbSNP:750901737
111 111 a, g dbSNP:765772946
112 112 c, t dbSNP:762144588
119 119 c, t dbSNP:754181875
138 138 c, t dbSNP:779788217
139 139 a, g dbSNP:3213096
150 150 c, t dbSNP:749937888
157 157 c, g dbSNP:779340279
158 158 c, t dbSNP:757751382
165 165 a, c dbSNP:754304446
169 169 a, g dbSNP:144020395
178 178 a, g dbSNP:756413190
180 180 a, g dbSNP:776253449
181 181 a, g, t dbSNP:563294089
188 188 c, t dbSNP:56347721
197 197 a, c dbSNP:140131034
199 199 c, g, t dbSNP:183111978
218 218 c, t dbSNP:765124116
223 223 a, g dbSNP:761837865
231 231 c, t dbSNP:776655957
233 233 a, g dbSNP:150659914
237 237 c, t dbSNP:768578364
239 239 a, g dbSNP:746799998
250 250 a, g dbSNP:201684769
252 252 c, t dbSNP:771734221
263 263 a, c dbSNP:745410494
271 271 a, g dbSNP:778518679
273 273 a, c dbSNP:563992321
282 282 a, g dbSNP:749808063
284 284 a, g dbSNP:540842370
289 289 a, g dbSNP:756537897
308 308 c, t dbSNP:753044197
320 320 a, g dbSNP:267600520
324 324 c, t dbSNP:142017503
325 325 a, g dbSNP:56287471
327 327 c, g dbSNP:751689646
328 328 g, t dbSNP:368468349
335 335 a, g dbSNP:763062544
340 340 -, tcgctcct dbSNP:786201006
341 341 c, t dbSNP:144694601
342 342 a, g dbSNP:142998256
343 343 c, t dbSNP:200722574
361 361 -, a dbSNP:587776807
364 364 c, g dbSNP:775325033
365 365 a, g dbSNP:771727370
369 369 c, t dbSNP:745557945
373 373 a, g dbSNP:773815651
389 389 -, t dbSNP:11317523
392 392 a, t dbSNP:375191855
397 397 g, t dbSNP:748791854
400 400 a, c dbSNP:778309275
409 409 c, g dbSNP:780376200
413 413 a, g dbSNP:199703726
424 424 a, t dbSNP:750634348
425 425 c, g, t dbSNP:183576218
426 426 -, t dbSNP:779665906
435 435 c, t dbSNP:757355295
436 436 a, g dbSNP:139186048
457 457 c, t dbSNP:767350741
458 458 a, g dbSNP:759434204
471 471 a, g dbSNP:751288779
477 477 a, g dbSNP:56051849
479 479 c, t dbSNP:766056574
480 480 a, g dbSNP:569226644
481 481 a, g dbSNP:772801025
483 483 a, c dbSNP:140642018
487 487 a, t dbSNP:368021638
489 489 c, t dbSNP:761228891
490 490 a, g dbSNP:777255655
491 491 c, t dbSNP:373631126
498 498 a, g dbSNP:769025565
501 501 a, t dbSNP:747444855
510 510 -, gtc dbSNP:757693618
523 523 a, g dbSNP:776029345
525 525 c, t dbSNP:761108550
526 526 c, t dbSNP:56369079
531 531 c, t dbSNP:776084353
540 540 a, c dbSNP:772571198
548 548 c, t dbSNP:565345523
549 549 a, g, t dbSNP:369160771
552 552 c, t dbSNP:771036480
553 553 a, g dbSNP:189313574
571 571 g, t dbSNP:777742449
573 573 a, t dbSNP:756166931
576 576 a, c, g dbSNP:749361927
578 578 c, g dbSNP:375159171
586 586 a, g dbSNP:10045130
597 597 a, g dbSNP:142400793
611 611 c, t dbSNP:764913762
612 612 a, c dbSNP:756894865
613 613 c, g dbSNP:753481336
630 630 c, g dbSNP:567462873
640 640 c, g dbSNP:763525702
643 643 c, g dbSNP:760165869
651 651 a, g dbSNP:776207541
656 656 a, g dbSNP:768122910
664 664 a, g dbSNP:760025343
665 665 c, t dbSNP:138127918
683 683 c, t dbSNP:771282654
684 684 c, t dbSNP:149273360
685 685 a, g dbSNP:79446920
693 693 a, g dbSNP:769766217
702 702 a, t dbSNP:748215576
711 711 c, t dbSNP:149027358
719 719 a, g dbSNP:55661460
720 720 c, g dbSNP:745769395
745 745 c, t dbSNP:776726308
750 750 c, t dbSNP:768677098
753 753 a, g dbSNP:199527043
761 761 a, t dbSNP:745818512
764 764 c, t dbSNP:575161274
772 772 a, g dbSNP:778843948
778 778 c, t dbSNP:372926766
780 780 a, g dbSNP:192540461
783 783 c, g dbSNP:749098724
790 790 c, t dbSNP:368458310
791 791 a, g dbSNP:74644143
797 797 g, t dbSNP:201757381
800 800 a, g dbSNP:780867719
801 801 g, t dbSNP:754475899
803 803 g, t dbSNP:752010709
804 804 a, c dbSNP:766902711
812 812 a, c, t dbSNP:750772366
820 820 a, g dbSNP:765482576
851 851 c, t dbSNP:762049572
858 858 a, g dbSNP:776775490
864 864 c, t dbSNP:764163895
865 865 a, g dbSNP:189324104
871 871 a, g dbSNP:372475701
882 882 c, g dbSNP:770877646
893 893 a, g dbSNP:111492985
896 896 -, a dbSNP:765340406
896 896 a, c dbSNP:749076435
902 902 a, g dbSNP:146408881
904 904 a, g dbSNP:776481806
905 905 c, g dbSNP:370904274
906 906 a, g dbSNP:144054146
908 908 g, t dbSNP:779642242
912 912 c, t dbSNP:139163137
914 914 c, t dbSNP:367828574
915 915 a, g dbSNP:144356274
916 916 g, t dbSNP:150030340
923 923 a, c dbSNP:757678113
924 924 c, t dbSNP:754181801
930 930 c, g, t dbSNP:374523632
932 932 c, t dbSNP:374661285
933 933 a, g dbSNP:186003445
934 934 g, t dbSNP:3213119
939 939 c, t dbSNP:150902695
940 940 g, t dbSNP:750452400
943 943 c, t dbSNP:780789325
944 944 a, g dbSNP:377511395
956 956 a, g dbSNP:776601045
963 963 c, t dbSNP:768523225
964 964 g, t dbSNP:373042362
967 967 a, c, t dbSNP:771683283
975 975 a, c, g dbSNP:571398445
978 978 c, t dbSNP:776936485
979 979 c, t dbSNP:371280399
980 980 a, g dbSNP:377524637
986 986 a, g dbSNP:778129509
990 990 c, t dbSNP:756372086
1002 1002 c, t dbSNP:752964137
1003 1003 a, g dbSNP:201512006
1015 1015 a, g dbSNP:56064925
1023 1023 a, c, t dbSNP:138991749
1024 1024 a, c dbSNP:143277765
1029 1029 a, g dbSNP:761865432
1030 1030 a, g dbSNP:3213120
1097 1097 c, t dbSNP:528851700
1114 1114 a, g dbSNP:569969190
1125 1125 c, t dbSNP:549642878
1143 1143 -, t dbSNP:3213112
1143 1143 a, g dbSNP:754965668
1144 1144 -, t dbSNP:397840403
1150 1150 -, t dbSNP:530595443
1165 1165 a, c dbSNP:533007211
1188 1188 a, c dbSNP:3212227
1189 1189 a, g dbSNP:748031214
1217 1217 a, c dbSNP:1805132
1253 1253 c, t dbSNP:532345378
1254 1254 a, c dbSNP:3213113
1258 1258 c, g dbSNP:55949756
1259 1259 c, t dbSNP:56396672
1321 1321 c, t dbSNP:186528423
1322 1322 a, g dbSNP:561157719
1353 1353 c, t dbSNP:542242777
1359 1359 c, t dbSNP:529769729
1369 1369 g, t dbSNP:41412344
1380 1380 c, g dbSNP:568500425
1386 1386 c, g dbSNP:550205140
1409 1409 a, g dbSNP:760748365
1437 1437 c, t dbSNP:181215530
1445 1445 c, t dbSNP:188784749
1475 1475 g, t dbSNP:773407574
1488 1488 c, t dbSNP:546509210
1526 1526 c, t dbSNP:528573168
1533 1533 c, g dbSNP:778671255
1549 1549 c, t dbSNP:3213114
1559 1559 c, t dbSNP:139879928
1587 1587 a, c dbSNP:146117745
1642 1642 a, g dbSNP:531981828
1657 1657 c, t dbSNP:762130179
1677 1677 c, g dbSNP:534106315
1703 1703 c, t dbSNP:774743402
1713 1713 -, a dbSNP:56390703
1717 1717 a, c, t dbSNP:144366632
1725 1725 a, c, g dbSNP:369157824
1728 1728 a, g dbSNP:139698981
1745 1745 a, c dbSNP:41454346
1748 1748 a, g dbSNP:376593057
1775 1775 c, t dbSNP:41292468
1780 1780 c, t dbSNP:746568584
1781 1781 a, g dbSNP:569548120
1809 1809 a, g dbSNP:549706242
1823 1823 a, g dbSNP:765892969
1869 1869 a, g dbSNP:563941424
1912 1912 a, c dbSNP:56409614
1930 1930 -, a dbSNP:34324765
1957 1957 c, t dbSNP:750061605
1960 1960 c, t dbSNP:55876812
1973 1973 c, t dbSNP:767262445
1999 1999 c, g dbSNP:545035344
2009 2009 a, c dbSNP:185933681
2020 2020 -, t dbSNP:56081148
2045 2045 a, t dbSNP:547204212
2098 2098 g, t dbSNP:527479600
2124 2124 a, c dbSNP:1368439
2144 2144 c, g dbSNP:754804497
2168 2168 c, t dbSNP:369997793
2209 2209 a, c dbSNP:559187957
2215 2215 g, t dbSNP:181807125
2221 2221 g, t dbSNP:531770868
2223 2223 a, g dbSNP:562878121
2226 2226 -, g dbSNP:774569234
2229 2229 c, t dbSNP:546174255
2251 2251 c, t dbSNP:144817848
2256 2256 c, t dbSNP:560735709
2271 2271 c, t dbSNP:540857937
2341 2341 a, t dbSNP:574914587

Target ORF information:

RefSeq Version NM_002187
Organism Homo sapiens (human)
Definition Homo sapiens interleukin 12B (IL12B), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002187

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTGTCACC AGCAGTTGGT CATCTCTTGG TTTTCCCTGG TTTTTCTGGC ATCTCCCCTC 
GTGGCCATAT GGGAACTGAA GAAAGATGTT TATGTCGTAG AATTGGATTG GTATCCGGAT
GCCCCTGGAG AAATGGTGGT CCTCACCTGT GACACCCCTG AAGAAGATGG TATCACCTGG
ACCTTGGACC AGAGCAGTGA GGTCTTAGGC TCTGGCAAAA CCCTGACCAT CCAAGTCAAA
GAGTTTGGAG ATGCTGGCCA GTACACCTGT CACAAAGGAG GCGAGGTTCT AAGCCATTCG
CTCCTGCTGC TTCACAAAAA GGAAGATGGA ATTTGGTCCA CTGATATTTT AAAGGACCAG
AAAGAACCCA AAAATAAGAC CTTTCTAAGA TGCGAGGCCA AGAATTATTC TGGACGTTTC
ACCTGCTGGT GGCTGACGAC AATCAGTACT GATTTGACAT TCAGTGTCAA AAGCAGCAGA
GGCTCTTCTG ACCCCCAAGG GGTGACGTGC GGAGCTGCTA CACTCTCTGC AGAGAGAGTC
AGAGGGGACA ACAAGGAGTA TGAGTACTCA GTGGAGTGCC AGGAGGACAG TGCCTGCCCA
GCTGCTGAGG AGAGTCTGCC CATTGAGGTC ATGGTGGATG CCGTTCACAA GCTCAAGTAT
GAAAACTACA CCAGCAGCTT CTTCATCAGG GACATCATCA AACCTGACCC ACCCAAGAAC
TTGCAGCTGA AGCCATTAAA GAATTCTCGG CAGGTGGAGG TCAGCTGGGA GTACCCTGAC
ACCTGGAGTA CTCCACATTC CTACTTCTCC CTGACATTCT GCGTTCAGGT CCAGGGCAAG
AGCAAGAGAG AAAAGAAAGA TAGAGTCTTC ACGGACAAGA CCTCAGCCAC GGTCATCTGC
CGCAAAAATG CCAGCATTAG CGTGCGGGCC CAGGACCGCT ACTATAGCTC ATCTTGGAGC
GAATGGGCAT CTGTGCCCTG CAGTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.