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INS insulin [Homo sapiens (human)]

Gene Symbol INS
Entrez Gene ID 3630
Full Name insulin
Synonyms IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10
General protein information
Preferred Names
insulin
Names
insulin
proinsulin
preproinsulin
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11p15.5

Summary After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]. lac of sum
Disorder MIM:

176730

Disorder Html: Hyperproinsulinemia, familial, with or without diabetes (3);





The following INS gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the INS gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu22288 NM_001185097 Homo sapiens insulin (INS), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $99
OHu22288 NM_001185098 Homo sapiens insulin (INS), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $99
OHu22288 NM_000207 Homo sapiens insulin (INS), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $99
OHu22288 NM_001291897 Homo sapiens insulin (INS), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu22288
Accession Version NM_001185097.1
Sequence Information ORF Nucleotide Sequence (Length: 333bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product insulin preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP322143.1, AY899304.1 and BM510347.1. Summary: After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. All variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU783312.1, BQ787808.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151405, SAMEA2153347 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001172026.1
CDS86..418
Misc Feature(1)161..415(+)
Misc Feature(2)179..409(+)
Exon (1)1..68
Gene:INS
Gene Synonym:
Exon (2)69..272
Gene:INS
Gene Synonym:
Exon (3)273..491
Gene:INS
Gene Synonym:
Translation
Position Chain Variation Link
14 14 a, g dbSNP:772426967
46 46 -, ttgc dbSNP:3842740
62 62 g, t dbSNP:539346403
63 63 c, t dbSNP:568856178
77 77 c, t dbSNP:5505
78 78 c, t dbSNP:11557611
79 79 c, t dbSNP:11557605
86 86 a, g dbSNP:757124361
88 88 a, g, t dbSNP:397515521
89 89 a, g dbSNP:535989053
91 91 c, t dbSNP:764207450
101 101 c, g, t dbSNP:121908278
102 102 a, g dbSNP:121908259
106 106 c, t dbSNP:759951127
110 110 c, t dbSNP:372122432
112 112 c, t dbSNP:11557610
120 120 c, t dbSNP:777079520
121 121 a, g dbSNP:3842744
122 122 c, t dbSNP:761260304
125 125 c, t dbSNP:774043636
133 133 c, g dbSNP:151134873
137 137 c, g dbSNP:748696269
147 147 c, t dbSNP:369743665
148 148 a, g dbSNP:11564720
150 150 c, t dbSNP:745671341
151 151 c, t dbSNP:375371953
152 152 a, g, t dbSNP:13306444
156 156 a, c, t dbSNP:80356663
157 157 a, c dbSNP:539284976
170 170 c, g dbSNP:121908272
178 178 c, t dbSNP:758540467
179 179 a, c, g dbSNP:80356664
185 185 c, g dbSNP:121918101
189 189 c, t dbSNP:121908273
198 198 c, t dbSNP:11557614
212 212 g, t dbSNP:80356666
214 214 c, t dbSNP:201392940
215 215 a, g dbSNP:765512575
217 217 a, g dbSNP:267602820
222 222 a, g dbSNP:121908260
223 223 a, g dbSNP:759819273
225 225 g, t dbSNP:80356667
226 226 c, t dbSNP:11557609
228 228 c, g, t dbSNP:80356668
232 232 c, g, t dbSNP:148685531
237 237 c, t dbSNP:556809279
238 238 a, c, g dbSNP:773789432
240 240 c, g dbSNP:145038693
242 242 a, g dbSNP:368338862
243 243 a, c dbSNP:202244834
247 247 c, t dbSNP:769540056
248 248 c, t dbSNP:121908261
250 250 c, t dbSNP:745511856
251 251 c, t dbSNP:781016664
258 258 c, t dbSNP:770737691
272 272 g, t dbSNP:746770373
273 273 c, t dbSNP:760240330
279 279 a, t dbSNP:773102009
280 280 a, g dbSNP:771743781
286 286 g, t dbSNP:41301451
287 287 a, c dbSNP:121908279
289 289 c, g dbSNP:774459022
292 292 c, t dbSNP:768647623
293 293 a, g dbSNP:749388720
297 297 g, t dbSNP:780264600
309 309 a, g dbSNP:144093133
312 312 a, g dbSNP:139264769
321 321 c, t dbSNP:781430273
327 327 c, t dbSNP:757595587
328 328 c, t dbSNP:751889371
332 332 a, g dbSNP:778270228
335 335 a, g dbSNP:121908274
339 339 a, c dbSNP:758893870
340 340 c, t dbSNP:530434985
349 349 a, g dbSNP:753412890
350 350 c, t dbSNP:80356669
351 351 a, c, g, t dbSNP:28933985
353 353 g, t dbSNP:80356670
354 354 a, g dbSNP:760425445
359 359 g, t dbSNP:121918102
364 364 a, g dbSNP:750063397
372 372 a, c, g dbSNP:80356671
387 387 c, g dbSNP:121908276
391 391 a, g dbSNP:11557607
393 393 a, g dbSNP:121908277
394 394 a, g dbSNP:11557608
398 398 a, g dbSNP:11557616
408 408 a, g dbSNP:80356672
420 420 c, t dbSNP:200306755
421 421 a, g dbSNP:761517339
422 422 a, g dbSNP:11557606
427 427 c, t dbSNP:3842752
428 428 a, g dbSNP:370330362
432 432 g, t dbSNP:762974872
440 440 a, c dbSNP:3842753
441 441 a, c, g dbSNP:745923821
442 442 a, c dbSNP:781277798
443 443 c, t dbSNP:369040062
445 445 c, t dbSNP:199866212
446 446 a, g dbSNP:778107057
448 448 a, c, t dbSNP:753183182
449 449 a, g dbSNP:779345894
454 454 c, t dbSNP:755609237
458 458 a, c, g dbSNP:767089366
460 460 c, t dbSNP:376867722
461 461 a, g dbSNP:192030414
462 462 a, g dbSNP:763947624
463 463 c, g dbSNP:763029961
471 471 a, g dbSNP:41313495
477 477 g, t dbSNP:11557615
477 477 a, g dbSNP:397515519
489 489 a, g dbSNP:769707738

Target ORF information:

RefSeq Version NM_001185097
Organism Homo sapiens (human)
Definition Homo sapiens insulin (INS), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001185097

ORF Insert Sequence:

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22288
Accession Version NM_001185098.1
Sequence Information ORF Nucleotide Sequence (Length: 333bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product insulin preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP322143.1, AC132217.15 and BM510347.1. Summary: After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## regulatory uORF :: PMID: 20628762 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BP322143.1, BU070847.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146236, SAMEA2147596 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001172027.1
CDS239..571
Misc Feature(1)147..158(+)
Misc Feature(2)314..568(+)
Misc Feature(3)332..562(+)
Exon (1)1..425
Gene:INS
Gene Synonym:
Exon (2)426..644
Gene:INS
Gene Synonym:
Translation
Position Chain Variation Link
14 14 a, g dbSNP:772426967
46 46 -, ttgc dbSNP:3842740
62 62 g, t dbSNP:539346403
63 63 c, t dbSNP:568856178
80 80 a, g dbSNP:3842741
91 91 a, g dbSNP:535108540
112 112 c, g dbSNP:748161866
121 121 g, t dbSNP:141890481
128 128 a, g dbSNP:9282754
140 140 a, g dbSNP:546344485
147 147 a, g dbSNP:528198568
148 148 g, t dbSNP:9282755
178 178 a, g dbSNP:372697376
188 188 c, t dbSNP:552050179
189 189 c, t dbSNP:748938752
198 198 -, gcct dbSNP:774301523
205 205 a, c, t dbSNP:200371131
211 211 c, t dbSNP:563207167
216 216 a, t dbSNP:689
230 230 c, t dbSNP:5505
231 231 c, t dbSNP:11557611
232 232 c, t dbSNP:11557605
239 239 a, g dbSNP:757124361
241 241 a, g, t dbSNP:397515521
242 242 a, g dbSNP:535989053
244 244 c, t dbSNP:764207450
254 254 c, g, t dbSNP:121908278
255 255 a, g dbSNP:121908259
259 259 c, t dbSNP:759951127
263 263 c, t dbSNP:372122432
265 265 c, t dbSNP:11557610
273 273 c, t dbSNP:777079520
274 274 a, g dbSNP:3842744
275 275 c, t dbSNP:761260304
278 278 c, t dbSNP:774043636
286 286 c, g dbSNP:151134873
290 290 c, g dbSNP:748696269
300 300 c, t dbSNP:369743665
301 301 a, g dbSNP:11564720
303 303 c, t dbSNP:745671341
304 304 c, t dbSNP:375371953
305 305 a, g, t dbSNP:13306444
309 309 a, c, t dbSNP:80356663
310 310 a, c dbSNP:539284976
323 323 c, g dbSNP:121908272
331 331 c, t dbSNP:758540467
332 332 a, c, g dbSNP:80356664
338 338 c, g dbSNP:121918101
342 342 c, t dbSNP:121908273
351 351 c, t dbSNP:11557614
365 365 g, t dbSNP:80356666
367 367 c, t dbSNP:201392940
368 368 a, g dbSNP:765512575
370 370 a, g dbSNP:267602820
375 375 a, g dbSNP:121908260
376 376 a, g dbSNP:759819273
378 378 g, t dbSNP:80356667
379 379 c, t dbSNP:11557609
381 381 c, g, t dbSNP:80356668
385 385 c, g, t dbSNP:148685531
390 390 c, t dbSNP:556809279
391 391 a, c, g dbSNP:773789432
393 393 c, g dbSNP:145038693
395 395 a, g dbSNP:368338862
396 396 a, c dbSNP:202244834
400 400 c, t dbSNP:769540056
401 401 c, t dbSNP:121908261
403 403 c, t dbSNP:745511856
404 404 c, t dbSNP:781016664
411 411 c, t dbSNP:770737691
425 425 g, t dbSNP:746770373
426 426 c, t dbSNP:760240330
432 432 a, t dbSNP:773102009
433 433 a, g dbSNP:771743781
439 439 g, t dbSNP:41301451
440 440 a, c dbSNP:121908279
442 442 c, g dbSNP:774459022
445 445 c, t dbSNP:768647623
446 446 a, g dbSNP:749388720
450 450 g, t dbSNP:780264600
462 462 a, g dbSNP:144093133
465 465 a, g dbSNP:139264769
474 474 c, t dbSNP:781430273
480 480 c, t dbSNP:757595587
481 481 c, t dbSNP:751889371
485 485 a, g dbSNP:778270228
488 488 a, g dbSNP:121908274
492 492 a, c dbSNP:758893870
493 493 c, t dbSNP:530434985
502 502 a, g dbSNP:753412890
503 503 c, t dbSNP:80356669
504 504 a, c, g, t dbSNP:28933985
506 506 g, t dbSNP:80356670
507 507 a, g dbSNP:760425445
512 512 g, t dbSNP:121918102
517 517 a, g dbSNP:750063397
525 525 a, c, g dbSNP:80356671
540 540 c, g dbSNP:121908276
544 544 a, g dbSNP:11557607
546 546 a, g dbSNP:121908277
547 547 a, g dbSNP:11557608
551 551 a, g dbSNP:11557616
561 561 a, g dbSNP:80356672
573 573 c, t dbSNP:200306755
574 574 a, g dbSNP:761517339
575 575 a, g dbSNP:11557606
580 580 c, t dbSNP:3842752
581 581 a, g dbSNP:370330362
585 585 g, t dbSNP:762974872
593 593 a, c dbSNP:3842753
594 594 a, c, g dbSNP:745923821
595 595 a, c dbSNP:781277798
596 596 c, t dbSNP:369040062
598 598 c, t dbSNP:199866212
599 599 a, g dbSNP:778107057
601 601 a, c, t dbSNP:753183182
602 602 a, g dbSNP:779345894
607 607 c, t dbSNP:755609237
611 611 a, c, g dbSNP:767089366
613 613 c, t dbSNP:376867722
614 614 a, g dbSNP:192030414
615 615 a, g dbSNP:763947624
616 616 c, g dbSNP:763029961
624 624 a, g dbSNP:41313495
630 630 g, t dbSNP:11557615
630 630 a, g dbSNP:397515519
642 642 a, g dbSNP:769707738

Target ORF information:

RefSeq Version NM_001185098
Organism Homo sapiens (human)
Definition Homo sapiens insulin (INS), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001185098

ORF Insert Sequence:

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22288
Accession Version NM_000207.2
Sequence Information ORF Nucleotide Sequence (Length: 333bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product insulin preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC005255.1 and BM510748.1. This sequence is a reference standard in the RefSeqGene project. On Jun 15, 2006 this sequence version replaced gi:4557670. Summary: After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]. Transcript Variant: This variant (1) represents the shortest variant. All variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005255.1, BM511097.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153347 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000198.1
CDS60..392
Misc Feature(1)132..134(+)
Misc Feature(2)132..134(+)
Misc Feature(3)135..389(+)
Misc Feature(4)141..143(+)
Misc Feature(5)153..383(+)
Misc Feature(6)156..158(+)
Misc Feature(7)162..164(+)
Misc Feature(8)168..170(+)
Misc Feature(9)168..170(+)
Misc Feature(10)171..173(+)
Misc Feature(11)174..176(+)
Misc Feature(12)177..179(+)
Misc Feature(13)177..179(+)
Misc Feature(14)180..182(+)
Misc Feature(15)192..194(+)
Misc Feature(16)198..200(+)
Misc Feature(17)201..203(+)
Misc Feature(18)201..203(+)
Misc Feature(19)204..206(+)
Misc Feature(20)222..224(+)
Misc Feature(21)324..326(+)
Exon (1)1..42
Gene:INS
Gene Synonym:
Exon (2)43..246
Gene:INS
Gene Synonym:
Exon (3)247..465
Gene:INS
Gene Synonym:
Translation
Position Chain Variation Link
14 14 a, g dbSNP:772426967
51 51 c, t dbSNP:5505
52 52 c, t dbSNP:11557611
53 53 c, t dbSNP:11557605
60 60 a, g dbSNP:757124361
62 62 a, g, t dbSNP:397515521
63 63 a, g dbSNP:535989053
65 65 c, t dbSNP:764207450
75 75 c, g, t dbSNP:121908278
76 76 a, g dbSNP:121908259
80 80 c, t dbSNP:759951127
84 84 c, t dbSNP:372122432
86 86 c, t dbSNP:11557610
94 94 c, t dbSNP:777079520
95 95 a, g dbSNP:3842744
96 96 c, t dbSNP:761260304
99 99 c, t dbSNP:774043636
107 107 c, g dbSNP:151134873
111 111 c, g dbSNP:748696269
121 121 c, t dbSNP:369743665
122 122 a, g dbSNP:11564720
124 124 c, t dbSNP:745671341
125 125 c, t dbSNP:375371953
126 126 a, g, t dbSNP:13306444
130 130 a, c, t dbSNP:80356663
131 131 a, c dbSNP:539284976
144 144 c, g dbSNP:121908272
152 152 c, t dbSNP:758540467
153 153 a, c, g dbSNP:80356664
159 159 c, g dbSNP:121918101
163 163 c, t dbSNP:121908273
172 172 c, t dbSNP:11557614
186 186 g, t dbSNP:80356666
188 188 c, t dbSNP:201392940
189 189 a, g dbSNP:765512575
191 191 a, g dbSNP:267602820
196 196 a, g dbSNP:121908260
197 197 a, g dbSNP:759819273
199 199 g, t dbSNP:80356667
200 200 c, t dbSNP:11557609
202 202 c, g, t dbSNP:80356668
206 206 c, g, t dbSNP:148685531
211 211 c, t dbSNP:556809279
212 212 a, c, g dbSNP:773789432
214 214 c, g dbSNP:145038693
216 216 a, g dbSNP:368338862
217 217 a, c dbSNP:202244834
221 221 c, t dbSNP:769540056
222 222 c, t dbSNP:121908261
224 224 c, t dbSNP:745511856
225 225 c, t dbSNP:781016664
232 232 c, t dbSNP:770737691
246 246 g, t dbSNP:746770373
247 247 c, t dbSNP:760240330
253 253 a, t dbSNP:773102009
254 254 a, g dbSNP:771743781
260 260 g, t dbSNP:41301451
261 261 a, c dbSNP:121908279
263 263 c, g dbSNP:774459022
266 266 c, t dbSNP:768647623
267 267 a, g dbSNP:749388720
271 271 g, t dbSNP:780264600
283 283 a, g dbSNP:144093133
286 286 a, g dbSNP:139264769
295 295 c, t dbSNP:781430273
301 301 c, t dbSNP:757595587
302 302 c, t dbSNP:751889371
306 306 a, g dbSNP:778270228
309 309 a, g dbSNP:121908274
313 313 a, c dbSNP:758893870
314 314 c, t dbSNP:530434985
323 323 a, g dbSNP:753412890
324 324 c, t dbSNP:80356669
325 325 a, c, g, t dbSNP:28933985
327 327 g, t dbSNP:80356670
328 328 a, g dbSNP:760425445
333 333 g, t dbSNP:121918102
338 338 a, g dbSNP:750063397
346 346 a, c, g dbSNP:80356671
361 361 c, g dbSNP:121908276
365 365 a, g dbSNP:11557607
367 367 a, g dbSNP:121908277
368 368 a, g dbSNP:11557608
372 372 a, g dbSNP:11557616
382 382 a, g dbSNP:80356672
394 394 c, t dbSNP:200306755
395 395 a, g dbSNP:761517339
396 396 a, g dbSNP:11557606
401 401 c, t dbSNP:3842752
402 402 a, g dbSNP:370330362
406 406 g, t dbSNP:762974872
414 414 a, c dbSNP:3842753
415 415 a, c, g dbSNP:745923821
416 416 a, c dbSNP:781277798
417 417 c, t dbSNP:369040062
419 419 c, t dbSNP:199866212
420 420 a, g dbSNP:778107057
422 422 a, c, t dbSNP:753183182
423 423 a, g dbSNP:779345894
428 428 c, t dbSNP:755609237
432 432 a, c, g dbSNP:767089366
434 434 c, t dbSNP:376867722
435 435 a, g dbSNP:192030414
436 436 a, g dbSNP:763947624
437 437 c, g dbSNP:763029961
445 445 a, g dbSNP:41313495
451 451 g, t dbSNP:11557615
451 451 a, g dbSNP:397515519
463 463 a, g dbSNP:769707738

Target ORF information:

RefSeq Version NM_000207
Organism Homo sapiens (human)
Definition Homo sapiens insulin (INS), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000207

ORF Insert Sequence:

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22288
Accession Version NM_001291897.1
Sequence Information ORF Nucleotide Sequence (Length: 333bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product insulin preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC132217.15 and BM510347.1. Summary: After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]. Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BI521182.1, BQ581792.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2146236, SAMEA2147596 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001278826.1
CDS120..452
Misc Feature(1)195..449(+)
Misc Feature(2)213..443(+)
Exon (1)1..102
Gene:INS
Gene Synonym:
Exon (2)103..306
Gene:INS
Gene Synonym:
Exon (3)307..525
Gene:INS
Gene Synonym:
Translation
Position Chain Variation Link
14 14 a, g dbSNP:772426967
46 46 -, ttgc dbSNP:3842740
62 62 g, t dbSNP:539346403
63 63 c, t dbSNP:568856178
80 80 a, g dbSNP:3842741
91 91 a, g dbSNP:535108540
111 111 c, t dbSNP:5505
112 112 c, t dbSNP:11557611
113 113 c, t dbSNP:11557605
120 120 a, g dbSNP:757124361
122 122 a, g, t dbSNP:397515521
123 123 a, g dbSNP:535989053
125 125 c, t dbSNP:764207450
135 135 c, g, t dbSNP:121908278
136 136 a, g dbSNP:121908259
140 140 c, t dbSNP:759951127
144 144 c, t dbSNP:372122432
146 146 c, t dbSNP:11557610
154 154 c, t dbSNP:777079520
155 155 a, g dbSNP:3842744
156 156 c, t dbSNP:761260304
159 159 c, t dbSNP:774043636
167 167 c, g dbSNP:151134873
171 171 c, g dbSNP:748696269
181 181 c, t dbSNP:369743665
182 182 a, g dbSNP:11564720
184 184 c, t dbSNP:745671341
185 185 c, t dbSNP:375371953
186 186 a, g, t dbSNP:13306444
190 190 a, c, t dbSNP:80356663
191 191 a, c dbSNP:539284976
204 204 c, g dbSNP:121908272
212 212 c, t dbSNP:758540467
213 213 a, c, g dbSNP:80356664
219 219 c, g dbSNP:121918101
223 223 c, t dbSNP:121908273
232 232 c, t dbSNP:11557614
246 246 g, t dbSNP:80356666
248 248 c, t dbSNP:201392940
249 249 a, g dbSNP:765512575
251 251 a, g dbSNP:267602820
256 256 a, g dbSNP:121908260
257 257 a, g dbSNP:759819273
259 259 g, t dbSNP:80356667
260 260 c, t dbSNP:11557609
262 262 c, g, t dbSNP:80356668
266 266 c, g, t dbSNP:148685531
271 271 c, t dbSNP:556809279
272 272 a, c, g dbSNP:773789432
274 274 c, g dbSNP:145038693
276 276 a, g dbSNP:368338862
277 277 a, c dbSNP:202244834
281 281 c, t dbSNP:769540056
282 282 c, t dbSNP:121908261
284 284 c, t dbSNP:745511856
285 285 c, t dbSNP:781016664
292 292 c, t dbSNP:770737691
306 306 g, t dbSNP:746770373
307 307 c, t dbSNP:760240330
313 313 a, t dbSNP:773102009
314 314 a, g dbSNP:771743781
320 320 g, t dbSNP:41301451
321 321 a, c dbSNP:121908279
323 323 c, g dbSNP:774459022
326 326 c, t dbSNP:768647623
327 327 a, g dbSNP:749388720
331 331 g, t dbSNP:780264600
343 343 a, g dbSNP:144093133
346 346 a, g dbSNP:139264769
355 355 c, t dbSNP:781430273
361 361 c, t dbSNP:757595587
362 362 c, t dbSNP:751889371
366 366 a, g dbSNP:778270228
369 369 a, g dbSNP:121908274
373 373 a, c dbSNP:758893870
374 374 c, t dbSNP:530434985
383 383 a, g dbSNP:753412890
384 384 c, t dbSNP:80356669
385 385 a, c, g, t dbSNP:28933985
387 387 g, t dbSNP:80356670
388 388 a, g dbSNP:760425445
393 393 g, t dbSNP:121918102
398 398 a, g dbSNP:750063397
406 406 a, c, g dbSNP:80356671
421 421 c, g dbSNP:121908276
425 425 a, g dbSNP:11557607
427 427 a, g dbSNP:121908277
428 428 a, g dbSNP:11557608
432 432 a, g dbSNP:11557616
442 442 a, g dbSNP:80356672
454 454 c, t dbSNP:200306755
455 455 a, g dbSNP:761517339
456 456 a, g dbSNP:11557606
461 461 c, t dbSNP:3842752
462 462 a, g dbSNP:370330362
466 466 g, t dbSNP:762974872
474 474 a, c dbSNP:3842753
475 475 a, c, g dbSNP:745923821
476 476 a, c dbSNP:781277798
477 477 c, t dbSNP:369040062
479 479 c, t dbSNP:199866212
480 480 a, g dbSNP:778107057
482 482 a, c, t dbSNP:753183182
483 483 a, g dbSNP:779345894
488 488 c, t dbSNP:755609237
492 492 a, c, g dbSNP:767089366
494 494 c, t dbSNP:376867722
495 495 a, g dbSNP:192030414
496 496 a, g dbSNP:763947624
497 497 c, g dbSNP:763029961
505 505 a, g dbSNP:41313495
511 511 g, t dbSNP:11557615
511 511 a, g dbSNP:397515519
523 523 a, g dbSNP:769707738

Target ORF information:

RefSeq Version NM_001291897
Organism Homo sapiens (human)
Definition Homo sapiens insulin (INS), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001291897

ORF Insert Sequence:

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.