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INS cDNA ORF clone, Homo sapiens (human)

Gene Symbol INS
Entrez Gene ID 3630
Full Name insulin
Synonyms IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10
General protein information
Preferred Names
insulin
Names
insulin
proinsulin
preproinsulin
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11p15.5

Summary After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]. lac of sum
Disorder MIM:

176730

Disorder Html: Hyperproinsulinemia, familial, with or without diabetes (3);

mRNA and Protein(s)

mRNA Protein Name
NM_001185097 NP_001172026 insulin preproprotein
NM_001185098 NP_001172027 insulin preproprotein
NM_000207 NP_000198 insulin preproprotein
NM_001291897 NP_001278826 insulin preproprotein



Homo sapiens (human) INS NP_000198.1
Pan troglodytes (chimpanzee) INS NP_001008996.1
Canis lupus familiaris (dog) INS NP_001123565.1
Mus musculus (house mouse) Ins2 NP_032413.1
Rattus norvegicus (Norway rat) Ins2 NP_062003.1
Gallus gallus (chicken) INS NP_990553.1
Danio rerio (zebrafish) ins NP_571131.1
Xenopus (Silurana) tropicalis (western clawed frog) ins NP_001093706.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following INS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the INS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu22288 NM_001185097 Homo sapiens insulin (INS), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
OHu22288 NM_001185098 Homo sapiens insulin (INS), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
OHu22288 NM_000207 Homo sapiens insulin (INS), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
OHu22288 NM_001291897 Homo sapiens insulin (INS), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22288
Clone ID Related Accession (Same CDS sequence) NM_000207 , NM_001185097 , NM_001185098 , NM_001291897
Accession Version NM_001185097.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 333bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product insulin preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP322143.1, AY899304.1 and BM510347.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. All variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU783312.1, BQ787808.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151405, SAMEA2153347 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001172026.1
CDS86..418
Misc Feature(1)161..415(+)
Misc Feature(2)179..409(+)
Exon (1)1..68
Gene:INS
Gene Synonym:
Exon (2)69..272
Gene:INS
Gene Synonym:
Exon (3)273..491
Gene:INS
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001185097
Organism Homo sapiens (human)
Definition Homo sapiens insulin (INS), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001185097

ORF Insert Sequence:

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22288
Clone ID Related Accession (Same CDS sequence) NM_000207 , NM_001185097 , NM_001185098 , NM_001291897
Accession Version NM_001185098.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 333bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product insulin preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP322143.1, AC132217.15 and BM510347.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## regulatory uORF :: PMID: 20628762 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BP322143.1, BU070847.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146236, SAMEA2147596 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001172027.1
CDS239..571
Misc Feature(1)147..158(+)
Misc Feature(2)314..568(+)
Misc Feature(3)332..562(+)
Exon (1)1..425
Gene:INS
Gene Synonym:
Exon (2)426..644
Gene:INS
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001185098
Organism Homo sapiens (human)
Definition Homo sapiens insulin (INS), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001185098

ORF Insert Sequence:

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22288
Clone ID Related Accession (Same CDS sequence) NM_000207 , NM_001185097 , NM_001185098 , NM_001291897
Accession Version NM_000207.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 333bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product insulin preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC005255.1 and BM510748.1. This sequence is a reference standard in the RefSeqGene project. On Jun 15, 2006 this sequence version replaced gi:4557670. Transcript Variant: This variant (1) represents the shortest variant. All variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005255.1, BM511097.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153347 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000198.1
CDS60..392
Misc Feature(1)132..134(+)
Misc Feature(2)132..134(+)
Misc Feature(3)135..389(+)
Misc Feature(4)141..143(+)
Misc Feature(5)153..383(+)
Misc Feature(6)156..158(+)
Misc Feature(7)162..164(+)
Misc Feature(8)168..170(+)
Misc Feature(9)168..170(+)
Misc Feature(10)171..173(+)
Misc Feature(11)174..176(+)
Misc Feature(12)177..179(+)
Misc Feature(13)177..179(+)
Misc Feature(14)180..182(+)
Misc Feature(15)192..194(+)
Misc Feature(16)198..200(+)
Misc Feature(17)201..203(+)
Misc Feature(18)201..203(+)
Misc Feature(19)204..206(+)
Misc Feature(20)222..224(+)
Misc Feature(21)324..326(+)
Exon (1)1..42
Gene:INS
Gene Synonym:
Exon (2)43..246
Gene:INS
Gene Synonym:
Exon (3)247..465
Gene:INS
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000207
Organism Homo sapiens (human)
Definition Homo sapiens insulin (INS), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000207

ORF Insert Sequence:

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22288
Clone ID Related Accession (Same CDS sequence) NM_000207 , NM_001185097 , NM_001185098 , NM_001291897
Accession Version NM_001291897.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 333bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product insulin preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC132217.15 and BM510347.1. Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BI521182.1, BQ581792.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2146236, SAMEA2147596 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001278826.1
CDS120..452
Misc Feature(1)195..449(+)
Misc Feature(2)213..443(+)
Exon (1)1..102
Gene:INS
Gene Synonym:
Exon (2)103..306
Gene:INS
Gene Synonym:
Exon (3)307..525
Gene:INS
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001291897
Organism Homo sapiens (human)
Definition Homo sapiens insulin (INS), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001291897

ORF Insert Sequence:

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Impaired incretin secretion and pancreatic dysfunction with older age and diabetes
Metab. Clin. Exp. 63 (7), 922-929 (2014)
Geloneze B, de Oliveira Mda S, Vasques AC, Novaes FS, Pareja JC and Tambascia MA.


book

Ketosis onset type 2 diabetes had better islet beta-cell function and more serious insulin resistance
J Diabetes Res 2014, 510643 (2014)
Lu H, Hu F, Zeng Y, Zou L, Luo S, Sun Y, Liu H and Sun L.


book

Impaired beta cell function in Chinese newly diagnosed type 2 diabetes mellitus with hyperlipidemia
J Diabetes Res 2014, 493039 (2014)
Ma Y, Wang Y, Huang Q, Ren Q, Chen S, Zhang A, Zhao L, Zhen Q and Peng Y.


book

Allele-specific recognition of the 3' splice site of INS intron 1
Hum. Genet. 128 (4), 383-400 (2010)
Kralovicova J and Vorechovsky I.


book

Global haplotype diversity in the human insulin gene region
Genome Res. 13 (9), 2101-2111 (2003)
Stead JD, Hurles ME and Jeffreys AJ.


book

Permanent Neonatal Diabetes Mellitus
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
De Leon,D.D. and Stanley,C.A.


book

Nucleotide sequence of a cDNA clone encoding human preproinsulin
Nature 282 (5738), 525-527 (1979)
Bell,G.I., Swain,W.F., Pictet,R., Cordell,B., Goodman,H.M. and Rutter,W.J.


book

A structurally abnormal insulin causing human diabetes
Nature 281 (5727), 122-125 (1979)
Tager,H., Given,B., Baldwin,D., Mako,M., Markese,J., Rubenstein,A., Olefsky,J., Kobayashi,M., Kolterman,O. and Poucher,R.


book

Effects of altered thyroid function on galactosyl diacylglycerol metabolism in myelinating rat brain
J. Biol. Chem. 252 (16), 5864-5870 (1977)
Flynn,T.J., Deshmukh,D.S. and Pieringer,R.A.


book

Chemistry of insulin; determination of the structure of insulin opens the way to greater understanding of life processes
Science 129 (3359), 1340-1344 (1959)
SANGER,F.


 
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