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PDX1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol PDX1
Entrez Gene ID 3651
Full Name pancreatic and duodenal homeobox 1
Synonyms GSF, IDX-1, IPF1, IUF1, MODY4, PAGEN1, PDX-1, STF-1
General protein information
Preferred Names
pancreas/duodenum homeobox protein 1
Names
pancreas/duodenum homeobox protein 1
IPF-1
IUF-1
glucose-sensitive factor
insulin upstream factor 1
islet/duodenum homeobox-1
somatostatin transcription factor 1
pancreatic-duodenal homeobox factor 1
somatostatin-transactivating factor 1
insulin promoter factor 1, homeodomain transcription factor
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

13

13q12.1

Summary The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4). [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

600733

Disorder Html: Pancreatic agenesis, 260370 (3); MODY, type IV, 606392 (3); {Diabetes

mRNA and Protein(s)

mRNA Protein Name
NM_000209 NP_000200 pancreas/duodenum homeobox protein 1



Homo sapiens (human) PDX1 NP_000200.1
Pan troglodytes (chimpanzee) PDX1 NP_001074947.1
Macaca mulatta (Rhesus monkey) PDX1 XP_001096758.1
Canis lupus familiaris (dog) PDX1 XP_543155.3
Bos taurus (cattle) PDX1 NP_001179065.1
Mus musculus (house mouse) Pdx1 NP_032840.1
Rattus norvegicus (Norway rat) Pdx1 NP_074043.3
Gallus gallus (chicken) PDX1 XP_001234636.2
Danio rerio (zebrafish) pdx1 NP_571518.2
Xenopus (Silurana) tropicalis (western clawed frog) pdx1 XP_002934065.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PDX1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDX1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19441
NM_000209 Homo sapiens pancreatic and duodenal homeobox 1 (PDX1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$154.50-$216.30
$309.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19441
Clone ID Related Accession (Same CDS sequence) NM_000209
Accession Version NM_000209.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 852bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pancreas/duodenum homeobox protein 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CA848737.1, U30329.1 and AL353195.14. This sequence is a reference standard in the RefSeqGene project. On May 28, 2008 this sequence version replaced gi:119392075. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CA848737.1, X99894.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGAACGGCG AGGAGCAGTA CTACGCGGCC ACGCAGCTTT ACAAGGACCC ATGCGCGTTC 
CAGCGAGGCC CGGCGCCGGA GTTCAGCGCC AGCCCCCCTG CGTGCCTGTA CATGGGCCGC
CAGCCCCCGC CGCCGCCGCC GCACCCGTTC CCTGGCGCCC TGGGCGCGCT GGAGCAGGGC
AGCCCCCCGG ACATCTCCCC GTACGAGGTG CCCCCCCTCG CCGACGACCC CGCGGTGGCG
CACCTTCACC ACCACCTCCC GGCTCAGCTC GCGCTCCCCC ACCCGCCCGC CGGGCCCTTC
CCGGAGGGAG CCGAGCCGGG CGTCCTGGAG GAGCCCAACC GCGTCCAGCT GCCTTTCCCA
TGGATGAAGT CTACCAAAGC TCACGCGTGG AAAGGCCAGT GGGCAGGCGG CGCCTACGCT
GCGGAGCCGG AGGAGAACAA GCGGACGCGC ACGGCCTACA CGCGCGCACA GCTGCTAGAG
CTGGAGAAGG AGTTCCTATT CAACAAGTAC ATCTCACGGC CGCGCCGGGT GGAGCTGGCT
GTCATGTTGA ACTTGACCGA GAGACACATC AAGATCTGGT TCCAAAACCG CCGCATGAAG
TGGAAAAAGG AGGAGGACAA GAAGCGCGGC GGCGGGACAG CTGTCGGGGG TGGCGGGGTC
GCGGAGCCTG AGCAGGACTG CGCCGTGACC TCCGGCGAGG AGCTTCTGGC GCTGCCGCCG
CCGCCGCCCC CCGGAGGTGC TGTGCCGCCC GCTGCCCCCG TTGCCGCCCG AGAGGGCCGC
CTGCCGCCTG GCCTTAGCGC GTCGCCACAG CCCTCCAGCG TCGCGCCTCG GCGGCCGCAG
GAACCACGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000200.1
CDS109..960
Misc Feature(1)145..327(+)
Misc Feature(2)460..477(+)
Misc Feature(3)547..723(+)
Misc Feature(4)547..717(+)
Misc Feature(5)553..705(+)
Misc Feature(6)697..717(+)
Misc Feature(7)910..912(+)
Exon (1)1..514
Gene:PDX1
Gene Synonym:
Exon (2)515..2573
Gene:PDX1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000209
Organism Homo sapiens (human)
Definition Homo sapiens pancreatic and duodenal homeobox 1 (PDX1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000209

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGAACGGCG AGGAGCAGTA CTACGCGGCC ACGCAGCTTT ACAAGGACCC ATGCGCGTTC 
CAGCGAGGCC CGGCGCCGGA GTTCAGCGCC AGCCCCCCTG CGTGCCTGTA CATGGGCCGC
CAGCCCCCGC CGCCGCCGCC GCACCCGTTC CCTGGCGCCC TGGGCGCGCT GGAGCAGGGC
AGCCCCCCGG ACATCTCCCC GTACGAGGTG CCCCCCCTCG CCGACGACCC CGCGGTGGCG
CACCTTCACC ACCACCTCCC GGCTCAGCTC GCGCTCCCCC ACCCGCCCGC CGGGCCCTTC
CCGGAGGGAG CCGAGCCGGG CGTCCTGGAG GAGCCCAACC GCGTCCAGCT GCCTTTCCCA
TGGATGAAGT CTACCAAAGC TCACGCGTGG AAAGGCCAGT GGGCAGGCGG CGCCTACGCT
GCGGAGCCGG AGGAGAACAA GCGGACGCGC ACGGCCTACA CGCGCGCACA GCTGCTAGAG
CTGGAGAAGG AGTTCCTATT CAACAAGTAC ATCTCACGGC CGCGCCGGGT GGAGCTGGCT
GTCATGTTGA ACTTGACCGA GAGACACATC AAGATCTGGT TCCAAAACCG CCGCATGAAG
TGGAAAAAGG AGGAGGACAA GAAGCGCGGC GGCGGGACAG CTGTCGGGGG TGGCGGGGTC
GCGGAGCCTG AGCAGGACTG CGCCGTGACC TCCGGCGAGG AGCTTCTGGC GCTGCCGCCG
CCGCCGCCCC CCGGAGGTGC TGTGCCGCCC GCTGCCCCCG TTGCCGCCCG AGAGGGCCGC
CTGCCGCCTG GCCTTAGCGC GTCGCCACAG CCCTCCAGCG TCGCGCCTCG GCGGCCGCAG
GAACCACGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Identification of low-frequency and rare sequence variants associated with elevated or reduced risk of type 2 diabetes
Nat. Genet. 46 (3), 294-298 (2014)
Steinthorsdottir V, Thorleifsson G, Sulem P, Helgason H, Grarup N, Sigurdsson A, Helgadottir HT, Johannsdottir H, Magnusson OT, Gudjonsson SA, Justesen JM, Harder MN, Jorgensen ME, Christensen C, Brandslund I, Sandbaek A, Lauritzen T, Vestergaard H, Linneberg A, Jorgensen T, Hansen T, Daneshpour MS, Fallah MS, Hreidarsson AB, Sigurdsson G, Azizi F, Benediktsson R, Masson G, Helgason A, Kong A, Gudbjartsson DF, Pedersen O, Thorsteinsdottir U and Stefansson K.


book

Thermodynamic and structural determinants of differential Pdx1 binding to elements from the insulin and IAPP promoters
J. Mol. Biol. 425 (18), 3360-3377 (2013)
Bastidas M and Showalter SA.


book

Per-Arnt-Sim kinase regulates pancreatic duodenal homeobox-1 protein stability via phosphorylation of glycogen synthase kinase 3beta in pancreatic beta-cells
J. Biol. Chem. 288 (34), 24825-24833 (2013)
Semache M, Zarrouki B, Fontes G, Fogarty S, Kikani C, Chawki MB, Rutter J and Poitout V.


book

Inactivation of specific beta cell transcription factors in type 2 diabetes
J. Clin. Invest. 123 (8), 3305-3316 (2013)
Guo,S., Dai,C., Guo,M., Taylor,B., Harmon,J.S., Sander,M., Robertson,R.P., Powers,A.C. and Stein,R.


book

Binding polymorphism in the DNA bound state of the Pdx1 homeodomain
PLoS Comput. Biol. 9 (8), E1003160 (2013)
Babin V, Wang D, Rose RB and Sagui C.


book

Transcriptional activation of the GLUT2 gene by the IPF-1/STF-1/IDX-1 homeobox factor
Mol. Endocrinol. 10 (11), 1327-1334 (1996)
Waeber G, Thompson N, Nicod P and Bonny C.


book

Isolation, characterization, and chromosomal mapping of the human insulin promoter factor 1 (IPF-1) gene
Diabetes 45 (6), 789-794 (1996)
Inoue H, Riggs AC, Tanizawa Y, Ueda K, Kuwano A, Liu L, Donis-Keller H and Permutt MA.


book

Localization of human homeodomain transcription factor insulin promoter factor 1 (IPF1) to chromosome band 13q12.1
Genomics 28 (1), 125-126 (1995)
Stoffel M, Stein R, Wright CV, Espinosa R 3rd, Le Beau MM and Bell GI.


book

Characterization of somatostatin transactivating factor-1, a novel homeobox factor that stimulates somatostatin expression in pancreatic islet cells
Mol. Endocrinol. 7 (10), 1275-1283 (1993)
Leonard J, Peers B, Johnson T, Ferreri K, Lee S and Montminy MR.


book

Permanent Neonatal Diabetes Mellitus
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
De Leon,D.D. and Stanley,C.A.


 
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