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CD82 CD82 molecule [Homo sapiens (human)]

Gene Symbol CD82
Entrez Gene ID 3732
Full Name CD82 molecule
Synonyms 4F9, C33, GR15, IA4, KAI1, R2, SAR2, ST6, TSPAN27
General protein information
Preferred Names
CD82 antigen
Names
CD82 antigen
tspan-27
C33 antigen
tetraspanin-27
inducible membrane protein R2
metastasis suppressor Kangai-1
kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4))
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11p11.2

Summary This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

600623

Disorder Html: {Prostate cancer, susceptibility to}, 176807 (2)





The following CD82 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CD82 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu44860 XM_006718222 PREDICTED: Homo sapiens CD82 molecule (CD82), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu26932 XM_006718223 PREDICTED: Homo sapiens CD82 molecule (CD82), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu58546 XM_011520067 PREDICTED: Homo sapiens CD82 molecule (CD82), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu26932 NM_002231 Homo sapiens CD82 molecule (CD82), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $309
OHu26919 NM_001024844 Homo sapiens CD82 molecule (CD82), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $269

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu44860
Accession Version XM_006718222.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 927bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product CD82 antigen isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCCCTGG TCATTAACCA GAGTCCTCCC TGCTGCTGTG TGGACGACAC GTGGGCACAG 
GCAGAAGTGG GCCCTGTGAC CAGCTGCACT GGTTTCGTGG AAGGAAGCTC CAGGACTGGC
GGGATGGGCT CAGCCTGTAT CAAAGTCACC AAATACTTTC TCTTCCTCTT CAACTTGATC
TTCTTTATCC TGGGCGCAGT GATCCTGGGC TTCGGGGTGT GGATCCTGGC CGACAAGAGC
AGTTTCATCT CTGTCCTGCA AACCTCCTCC AGCTCGCTTA GGATGGGGGC CTATGTCTTC
ATCGGCGTGG GGGCAGTCAC TATGCTCATG GGCTTCCTGG GCTGCATCGG CGCCGTCAAC
GAGGTCCGCT GCCTGCTGGG GCTGTACTTT GCTTTCCTGC TCCTGATCCT CATTGCCCAG
GTGACGGCCG GGGCCCTCTT CTACTTCAAC ATGGGCAAGC TGAAGCAGGA GATGGGCGGC
ATCGTGACTG AGCTCATTCG AGACTACAAC AGCAGTCGCG AGGACAGCCT GCAGGATGCC
TGGGACTACG TGCAGGCTCA GGTGAAGTGC TGCGGCTGGG TCAGCTTCTA CAACTGGACA
GACAACGCTG AGCTCATGAA TCGCCCTGAG GTCACCTACC CCTGTTCCTG CGAAGTCAAG
GGGGAAGAGG ACAACAGCCT TTCTGTGAGG AAGGGCTTCT GCGAGGCCCC CGGCAACAGG
ACCCAGAGTG GCAACCACCC TGAGGACTGG CCTGTGTACC AGGAGGGCTG CATGGAGAAG
GTGCAGGCGT GGCTGCAGGA GAACCTGGGC ATCATCCTCG GCGTGGGCGT GGGTGTGGCC
ATCATCGAGC TCCTGGGGAT GGTCCTGTCC ATCTGCTTGT GCCGGCACGT CCATTCCGAA
GACTACAGCA AGGTCCCCAA GTACTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006718285.1
CDS56..982
Misc Feature(1)248..946(+)
Misc Feature(2)494..865(+)
Misc Feature(3)500..619(+)
Translation
Position Chain Variation Link
20 20 a, g dbSNP:372733853
21 21 c, t dbSNP:77359459
27 27 c, t dbSNP:779582246
89 89 a, t dbSNP:754565162
91 91 c, t dbSNP:760477547
100 100 c, t dbSNP:552512734
101 101 a, g dbSNP:747933620
106 106 a, g dbSNP:764119634
124 124 g, t dbSNP:532951527
143 143 a, g dbSNP:769734246
151 151 c, t dbSNP:776219159
155 155 g, t dbSNP:11541050
162 162 c, g dbSNP:777055402
166 166 c, t dbSNP:762212914
175 175 c, t dbSNP:200238062
176 176 a, g dbSNP:773819071
178 178 a, g dbSNP:763522693
179 179 a, g, t dbSNP:765963603
182 182 a, g dbSNP:111718702
183 183 a, g dbSNP:767017762
186 186 c, t dbSNP:752510747
215 215 a, c dbSNP:761257124
217 217 a, c dbSNP:777513108
220 220 a, c dbSNP:749268925
230 230 c, t dbSNP:752146609
235 235 a, c dbSNP:372259856
244 244 c, t dbSNP:774679121
248 248 a, g dbSNP:760055528
250 250 c, t dbSNP:535717592
251 251 a, g dbSNP:774964064
253 253 a, g dbSNP:760383801
255 255 c, t dbSNP:749539429
264 264 c, g dbSNP:763666572
269 269 a, g dbSNP:554121820
271 271 a, g dbSNP:761468393
273 273 c, t dbSNP:148396643
282 282 c, t dbSNP:377535419
286 286 c, t dbSNP:138833231
287 287 a, g dbSNP:758337930
297 297 a, c, g dbSNP:781480823
302 302 a, g dbSNP:756411683
308 308 a, g dbSNP:778085878
314 314 c, t dbSNP:150533529
321 321 c, t dbSNP:752840514
330 330 a, c dbSNP:139889503
331 331 a, g dbSNP:777966335
334 334 c, t dbSNP:374050393
337 337 g, t dbSNP:201294710
347 347 c, t dbSNP:371929631
349 349 c, t dbSNP:143270517
350 350 a, g dbSNP:375078073
358 358 c, g, t dbSNP:146594387
361 361 a, c, g dbSNP:372931321
364 364 a, g dbSNP:34170190
376 376 c, t dbSNP:375797501
377 377 a, g dbSNP:769486957
383 383 a, c, t dbSNP:562318494
391 391 c, t dbSNP:149844780
399 399 g, t dbSNP:774467192
403 403 c, g dbSNP:759521623
404 404 a, g, t dbSNP:369269460
409 409 c, t dbSNP:757425084
414 414 a, g dbSNP:765440814
416 416 a, g dbSNP:145881169
422 422 c, g dbSNP:758720179
423 423 a, g dbSNP:148925723
428 428 c, t dbSNP:747565395
430 430 g, t dbSNP:370728156
436 436 g, t dbSNP:35348953
472 472 a, c dbSNP:766789029
474 474 a, c dbSNP:751917502
482 482 g, t dbSNP:756306249
483 483 a, c dbSNP:781684485
485 485 a, g dbSNP:574662941
488 488 a, g dbSNP:755612375
496 496 c, t dbSNP:780007862
504 504 a, g dbSNP:546228845
507 507 c, t dbSNP:541705376
526 526 a, g dbSNP:2303865
528 528 c, t dbSNP:762967350
529 529 a, g dbSNP:374008730
530 530 a, g dbSNP:774518909
532 532 c, t dbSNP:883752
533 533 a, g dbSNP:368613608
536 536 a, g dbSNP:752996542
538 538 c, t dbSNP:761244897
541 541 a, g dbSNP:2303864
548 548 c, t dbSNP:138793220
555 555 a, g dbSNP:756787839
557 557 a, g dbSNP:764806308
560 560 -, acaa dbSNP:755032584
561 561 a, g dbSNP:750138207
562 562 c, t dbSNP:757975489
563 563 a, g dbSNP:779968308
565 565 c, t dbSNP:746715629
570 570 g, t dbSNP:754923253
572 572 c, t dbSNP:142819851
573 573 a, g, t dbSNP:117157354
574 574 c, t dbSNP:774394673
575 575 a, g, t dbSNP:746166676
577 577 g, t dbSNP:11541051
603 603 a, c dbSNP:775933913
604 604 c, t dbSNP:573753161
605 605 a, g dbSNP:200277144
607 607 g, t dbSNP:781269208
608 608 c, g dbSNP:745695307
611 611 g, t dbSNP:777316375
614 614 -, aggtg dbSNP:778999473
615 615 a, g dbSNP:762386509
618 618 a, t dbSNP:751215015
628 628 a, c, t dbSNP:150371864
629 629 a, g dbSNP:752419097
635 635 a, g dbSNP:138109186
636 636 g, t dbSNP:756053924
637 637 c, t dbSNP:777519636
640 640 c, t dbSNP:750546226
646 646 c, t dbSNP:758448103
648 648 a, g dbSNP:780157672
655 655 a, c dbSNP:747290721
660 660 a, g dbSNP:373060622
661 661 c, t dbSNP:200149429
677 677 c, t dbSNP:149456679
678 678 a, g dbSNP:143845294
679 679 c, t dbSNP:541161774
681 681 c, t dbSNP:377503391
688 688 c, t dbSNP:267602880
696 696 c, t dbSNP:770399013
702 702 c, t dbSNP:773901832
706 706 a, c, t dbSNP:139816598
707 707 a, g dbSNP:370975589
713 713 a, g dbSNP:760418741
716 716 a, g dbSNP:763918347
717 717 a, g dbSNP:763742776
718 718 a, g dbSNP:753768500
719 719 a, g dbSNP:757203164
731 731 a, g dbSNP:780063147
732 732 a, g dbSNP:751624745
736 736 c, t dbSNP:766762693
738 738 c, t dbSNP:755265941
743 743 -, agga dbSNP:758151345
747 747 a, g dbSNP:781515941
750 750 a, g dbSNP:748555757
757 757 c, t dbSNP:141597594
758 758 a, g dbSNP:200920132
762 762 c, g, t dbSNP:749820676
766 766 a, c, t dbSNP:773861588
767 767 a, g dbSNP:771739391
777 777 c, t dbSNP:775162522
781 781 g, t dbSNP:760351929
786 786 g, t dbSNP:200514878
793 793 a, c dbSNP:776479933
810 810 g, t dbSNP:577477292
817 817 a, g dbSNP:765165459
827 827 a, g dbSNP:778562920
831 831 a, g dbSNP:146220817
836 836 a, g dbSNP:554846063
838 838 a, g dbSNP:542337864
839 839 a, c dbSNP:777719140
843 843 a, c, t dbSNP:201290700
844 844 a, g dbSNP:774382622
845 845 g, t dbSNP:372946885
855 855 a, g dbSNP:759367396
856 856 a, g dbSNP:74375347
860 860 c, t dbSNP:776567252
865 865 a, c dbSNP:762027127
868 868 c, t dbSNP:765384989
873 873 g, t dbSNP:750810564
874 874 c, t dbSNP:142423874
877 877 c, g, t dbSNP:79095986
878 878 a, g dbSNP:755430626
880 880 a, g, t dbSNP:780823917
883 883 -, gtgggt dbSNP:745383395
883 883 c, t dbSNP:190517526
884 884 a, g dbSNP:367797052
885 885 a, t dbSNP:748913627
887 887 g, t dbSNP:371497237
893 893 a, g dbSNP:778556850
898 898 a, c, t dbSNP:199858417
899 899 a, g dbSNP:1139971
901 901 c, g, t dbSNP:150062713
905 905 c, t dbSNP:754907833
913 913 g, t dbSNP:777837925
920 920 c, t dbSNP:749272629
922 922 c, g dbSNP:74885383
926 926 a, g dbSNP:778967682
937 937 c, t dbSNP:746076379
938 938 c, t dbSNP:201405906
939 939 a, g dbSNP:545998821
943 943 c, t dbSNP:761266322
944 944 a, c, g dbSNP:142351354
946 946 c, g dbSNP:7107335
952 952 a, c, g, t dbSNP:201650491
953 953 a, g dbSNP:766230830
961 961 c, t dbSNP:751291553
962 962 a, g dbSNP:754925345
968 968 c, g dbSNP:781061734
971 971 c, t dbSNP:147912305
972 972 c, t dbSNP:201671713
973 973 c, g dbSNP:148874083
978 978 a, g dbSNP:773237459
982 982 a, g dbSNP:772351881
983 983 a, g dbSNP:780577629
988 988 c, g dbSNP:747327385
994 994 c, t dbSNP:376795165
1001 1001 c, t dbSNP:777044032
1003 1003 c, t dbSNP:370925717
1007 1007 a, g, t dbSNP:376175476
1008 1008 c, t dbSNP:762518913
1012 1012 a, g dbSNP:765960487
1013 1013 c, g, t dbSNP:751381541
1016 1016 c, g dbSNP:767273646
1019 1019 a, g dbSNP:752630534
1026 1026 a, g dbSNP:756070640
1033 1033 a, c dbSNP:778859812
1047 1047 c, t dbSNP:547161316
1070 1070 c, g dbSNP:529985982
1098 1098 cg, tc dbSNP:386753094
1098 1098 c, t dbSNP:4755266
1099 1099 c, g dbSNP:77781241
1114 1114 a, g dbSNP:569628883
1115 1115 a, g dbSNP:371456705
1199 1199 a, g dbSNP:536686665
1200 1200 c, t dbSNP:374896443
1214 1214 c, t dbSNP:71490073
1216 1216 c, t dbSNP:555408480
1229 1229 a, c dbSNP:116250454
1245 1245 a, g dbSNP:536180457
1249 1249 c, t dbSNP:546842038
1256 1256 a, g dbSNP:554375084
1266 1266 c, t dbSNP:193127050
1306 1306 a, g dbSNP:778679874
1316 1316 a, c dbSNP:539902023
1320 1320 a, g dbSNP:745846614
1331 1331 a, g dbSNP:1063031
1338 1338 c, g dbSNP:12225877
1341 1341 a, g dbSNP:576375816
1344 1344 c, t dbSNP:543364816
1345 1345 a, g dbSNP:572260612
1347 1347 a, g dbSNP:538794913
1354 1354 c, g dbSNP:759814577
1356 1356 c, t dbSNP:540960667
1357 1357 c, g dbSNP:371743094
1364 1364 a, g dbSNP:533024020
1385 1385 a, g dbSNP:113805595
1390 1390 a, g dbSNP:559005209
1396 1396 a, g dbSNP:112667982
1401 1401 a, g dbSNP:563192606
1414 1414 c, t dbSNP:530636550
1415 1415 a, g dbSNP:538247800
1418 1418 a, c dbSNP:6485552
1423 1423 a, c dbSNP:370894393
1434 1434 c, t dbSNP:548974243
1445 1445 c, t dbSNP:374579809
1446 1446 a, g dbSNP:145762348
1449 1449 c, t dbSNP:536141761
1460 1460 c, t dbSNP:780025065
1469 1469 a, c dbSNP:367590200
1478 1478 a, g dbSNP:111384781
1482 1482 a, g dbSNP:143013555
1484 1484 a, g dbSNP:539686462
1489 1489 c, t dbSNP:558340117
1490 1490 a, g dbSNP:576431366
1493 1493 c, t dbSNP:537163605
1494 1494 a, g dbSNP:555325573
1513 1513 c, t dbSNP:573724569
1544 1544 a, c dbSNP:540921974
1545 1545 c, t dbSNP:559427355
1546 1546 a, g dbSNP:577875518
1551 1551 c, t dbSNP:190156561
1552 1552 a, g dbSNP:563522625
1563 1563 c, t dbSNP:761198379
1579 1579 c, t dbSNP:530589270
1581 1581 -, c dbSNP:374072292
1590 1590 -, gcctgg dbSNP:528703148
1598 1598 c, g dbSNP:549135777
1599 1599 g, t dbSNP:560848356
1613 1613 c, t dbSNP:528140057
1620 1620 -, aaa dbSNP:752801473
1620 1620 -, a dbSNP:561662297
1621 1621 -, aa dbSNP:775954230
1638 1638 -, aa dbSNP:398045166
1644 1644 -, aa dbSNP:397848957
1646 1646 c, t dbSNP:780140130
1662 1662 -, cg dbSNP:376197276
1662 1662 a, g dbSNP:146156139
1726 1726 a, c dbSNP:75976933
1751 1751 c, t dbSNP:566202585
1766 1766 a, c dbSNP:769396753
1790 1790 c, t dbSNP:540147029
1791 1791 c, t dbSNP:551648141
1803 1803 g, t dbSNP:762435369
1884 1884 c, t dbSNP:753710390
1885 1885 a, g dbSNP:570264504
1890 1890 a, g dbSNP:115328824
1911 1911 -, g dbSNP:34626122
1911 1911 g, t dbSNP:553986435
1921 1921 a, g dbSNP:754710250
1925 1925 c, g dbSNP:555485604
1928 1928 a, g dbSNP:140021217
1942 1942 c, g dbSNP:778723164
1957 1957 a, g dbSNP:111689312
1977 1977 a, g dbSNP:115940873
1992 1992 c, t dbSNP:763484969
2012 2012 a, g dbSNP:577833901
2027 2027 c, t dbSNP:546035719
2028 2028 a, g dbSNP:556863887
2040 2040 a, g dbSNP:772217498
2054 2054 a, g dbSNP:575540698
2078 2078 a, g dbSNP:181190575
2103 2103 g, t dbSNP:561007915
2128 2128 g, t dbSNP:528204027
2135 2135 g, t dbSNP:56028963
2146 2146 a, g dbSNP:564910931

Target ORF information:

RefSeq Version XM_006718222
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens CD82 molecule (CD82), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006718222

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCCCTGG TCATTAACCA GAGTCCTCCC TGCTGCTGTG TGGACGACAC GTGGGCACAG 
GCAGAAGTGG GCCCTGTGAC CAGCTGCACT GGTTTCGTGG AAGGAAGCTC CAGGACTGGC
GGGATGGGCT CAGCCTGTAT CAAAGTCACC AAATACTTTC TCTTCCTCTT CAACTTGATC
TTCTTTATCC TGGGCGCAGT GATCCTGGGC TTCGGGGTGT GGATCCTGGC CGACAAGAGC
AGTTTCATCT CTGTCCTGCA AACCTCCTCC AGCTCGCTTA GGATGGGGGC CTATGTCTTC
ATCGGCGTGG GGGCAGTCAC TATGCTCATG GGCTTCCTGG GCTGCATCGG CGCCGTCAAC
GAGGTCCGCT GCCTGCTGGG GCTGTACTTT GCTTTCCTGC TCCTGATCCT CATTGCCCAG
GTGACGGCCG GGGCCCTCTT CTACTTCAAC ATGGGCAAGC TGAAGCAGGA GATGGGCGGC
ATCGTGACTG AGCTCATTCG AGACTACAAC AGCAGTCGCG AGGACAGCCT GCAGGATGCC
TGGGACTACG TGCAGGCTCA GGTGAAGTGC TGCGGCTGGG TCAGCTTCTA CAACTGGACA
GACAACGCTG AGCTCATGAA TCGCCCTGAG GTCACCTACC CCTGTTCCTG CGAAGTCAAG
GGGGAAGAGG ACAACAGCCT TTCTGTGAGG AAGGGCTTCT GCGAGGCCCC CGGCAACAGG
ACCCAGAGTG GCAACCACCC TGAGGACTGG CCTGTGTACC AGGAGGGCTG CATGGAGAAG
GTGCAGGCGT GGCTGCAGGA GAACCTGGGC ATCATCCTCG GCGTGGGCGT GGGTGTGGCC
ATCATCGAGC TCCTGGGGAT GGTCCTGTCC ATCTGCTTGT GCCGGCACGT CCATTCCGAA
GACTACAGCA AGGTCCCCAA GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26932
Accession Version XM_006718223.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 804bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product CD82 antigen isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGCTCAG CCTGTATCAA AGTCACCAAA TACTTTCTCT TCCTCTTCAA CTTGATCTTC 
TTTATCCTGG GCGCAGTGAT CCTGGGCTTC GGGGTGTGGA TCCTGGCCGA CAAGAGCAGT
TTCATCTCTG TCCTGCAAAC CTCCTCCAGC TCGCTTAGGA TGGGGGCCTA TGTCTTCATC
GGCGTGGGGG CAGTCACTAT GCTCATGGGC TTCCTGGGCT GCATCGGCGC CGTCAACGAG
GTCCGCTGCC TGCTGGGGCT GTACTTTGCT TTCCTGCTCC TGATCCTCAT TGCCCAGGTG
ACGGCCGGGG CCCTCTTCTA CTTCAACATG GGCAAGCTGA AGCAGGAGAT GGGCGGCATC
GTGACTGAGC TCATTCGAGA CTACAACAGC AGTCGCGAGG ACAGCCTGCA GGATGCCTGG
GACTACGTGC AGGCTCAGGT GAAGTGCTGC GGCTGGGTCA GCTTCTACAA CTGGACAGAC
AACGCTGAGC TCATGAATCG CCCTGAGGTC ACCTACCCCT GTTCCTGCGA AGTCAAGGGG
GAAGAGGACA ACAGCCTTTC TGTGAGGAAG GGCTTCTGCG AGGCCCCCGG CAACAGGACC
CAGAGTGGCA ACCACCCTGA GGACTGGCCT GTGTACCAGG AGGGCTGCAT GGAGAAGGTG
CAGGCGTGGC TGCAGGAGAA CCTGGGCATC ATCCTCGGCG TGGGCGTGGG TGTGGCCATC
ATCGAGCTCC TGGGGATGGT CCTGTCCATC TGCTTGTGCC GGCACGTCCA TTCCGAAGAC
TACAGCAAGG TCCCCAAGTA CTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006718286.1
CDS156..959
Misc Feature(1)225..923(+)
Misc Feature(2)471..842(+)
Misc Feature(3)477..596(+)
Translation
Position Chain Variation Link
12 12 a, c dbSNP:772309528
13 13 c, t dbSNP:115500759
40 40 c, t dbSNP:576728423
66 66 a, t dbSNP:754565162
68 68 c, t dbSNP:760477547
77 77 c, t dbSNP:552512734
78 78 a, g dbSNP:747933620
83 83 a, g dbSNP:764119634
101 101 g, t dbSNP:532951527
120 120 a, g dbSNP:769734246
128 128 c, t dbSNP:776219159
132 132 g, t dbSNP:11541050
139 139 c, g dbSNP:777055402
143 143 c, t dbSNP:762212914
152 152 c, t dbSNP:200238062
153 153 a, g dbSNP:773819071
155 155 a, g dbSNP:763522693
156 156 a, g, t dbSNP:765963603
159 159 a, g dbSNP:111718702
160 160 a, g dbSNP:767017762
163 163 c, t dbSNP:752510747
192 192 a, c dbSNP:761257124
194 194 a, c dbSNP:777513108
197 197 a, c dbSNP:749268925
207 207 c, t dbSNP:752146609
212 212 a, c dbSNP:372259856
221 221 c, t dbSNP:774679121
225 225 a, g dbSNP:760055528
227 227 c, t dbSNP:535717592
228 228 a, g dbSNP:774964064
230 230 a, g dbSNP:760383801
232 232 c, t dbSNP:749539429
241 241 c, g dbSNP:763666572
246 246 a, g dbSNP:554121820
248 248 a, g dbSNP:761468393
250 250 c, t dbSNP:148396643
259 259 c, t dbSNP:377535419
263 263 c, t dbSNP:138833231
264 264 a, g dbSNP:758337930
274 274 a, c, g dbSNP:781480823
279 279 a, g dbSNP:756411683
285 285 a, g dbSNP:778085878
291 291 c, t dbSNP:150533529
298 298 c, t dbSNP:752840514
307 307 a, c dbSNP:139889503
308 308 a, g dbSNP:777966335
311 311 c, t dbSNP:374050393
314 314 g, t dbSNP:201294710
324 324 c, t dbSNP:371929631
326 326 c, t dbSNP:143270517
327 327 a, g dbSNP:375078073
335 335 c, g, t dbSNP:146594387
338 338 a, c, g dbSNP:372931321
341 341 a, g dbSNP:34170190
353 353 c, t dbSNP:375797501
354 354 a, g dbSNP:769486957
360 360 a, c, t dbSNP:562318494
368 368 c, t dbSNP:149844780
376 376 g, t dbSNP:774467192
380 380 c, g dbSNP:759521623
381 381 a, g, t dbSNP:369269460
386 386 c, t dbSNP:757425084
391 391 a, g dbSNP:765440814
393 393 a, g dbSNP:145881169
399 399 c, g dbSNP:758720179
400 400 a, g dbSNP:148925723
405 405 c, t dbSNP:747565395
407 407 g, t dbSNP:370728156
413 413 g, t dbSNP:35348953
449 449 a, c dbSNP:766789029
451 451 a, c dbSNP:751917502
459 459 g, t dbSNP:756306249
460 460 a, c dbSNP:781684485
462 462 a, g dbSNP:574662941
465 465 a, g dbSNP:755612375
473 473 c, t dbSNP:780007862
481 481 a, g dbSNP:546228845
484 484 c, t dbSNP:541705376
503 503 a, g dbSNP:2303865
505 505 c, t dbSNP:762967350
506 506 a, g dbSNP:374008730
507 507 a, g dbSNP:774518909
509 509 c, t dbSNP:883752
510 510 a, g dbSNP:368613608
513 513 a, g dbSNP:752996542
515 515 c, t dbSNP:761244897
518 518 a, g dbSNP:2303864
525 525 c, t dbSNP:138793220
532 532 a, g dbSNP:756787839
534 534 a, g dbSNP:764806308
537 537 -, acaa dbSNP:755032584
538 538 a, g dbSNP:750138207
539 539 c, t dbSNP:757975489
540 540 a, g dbSNP:779968308
542 542 c, t dbSNP:746715629
547 547 g, t dbSNP:754923253
549 549 c, t dbSNP:142819851
550 550 a, g, t dbSNP:117157354
551 551 c, t dbSNP:774394673
552 552 a, g, t dbSNP:746166676
554 554 g, t dbSNP:11541051
580 580 a, c dbSNP:775933913
581 581 c, t dbSNP:573753161
582 582 a, g dbSNP:200277144
584 584 g, t dbSNP:781269208
585 585 c, g dbSNP:745695307
588 588 g, t dbSNP:777316375
591 591 -, aggtg dbSNP:778999473
592 592 a, g dbSNP:762386509
595 595 a, t dbSNP:751215015
605 605 a, c, t dbSNP:150371864
606 606 a, g dbSNP:752419097
612 612 a, g dbSNP:138109186
613 613 g, t dbSNP:756053924
614 614 c, t dbSNP:777519636
617 617 c, t dbSNP:750546226
623 623 c, t dbSNP:758448103
625 625 a, g dbSNP:780157672
632 632 a, c dbSNP:747290721
637 637 a, g dbSNP:373060622
638 638 c, t dbSNP:200149429
654 654 c, t dbSNP:149456679
655 655 a, g dbSNP:143845294
656 656 c, t dbSNP:541161774
658 658 c, t dbSNP:377503391
665 665 c, t dbSNP:267602880
673 673 c, t dbSNP:770399013
679 679 c, t dbSNP:773901832
683 683 a, c, t dbSNP:139816598
684 684 a, g dbSNP:370975589
690 690 a, g dbSNP:760418741
693 693 a, g dbSNP:763918347
694 694 a, g dbSNP:763742776
695 695 a, g dbSNP:753768500
696 696 a, g dbSNP:757203164
708 708 a, g dbSNP:780063147
709 709 a, g dbSNP:751624745
713 713 c, t dbSNP:766762693
715 715 c, t dbSNP:755265941
720 720 -, agga dbSNP:758151345
724 724 a, g dbSNP:781515941
727 727 a, g dbSNP:748555757
734 734 c, t dbSNP:141597594
735 735 a, g dbSNP:200920132
739 739 c, g, t dbSNP:749820676
743 743 a, c, t dbSNP:773861588
744 744 a, g dbSNP:771739391
754 754 c, t dbSNP:775162522
758 758 g, t dbSNP:760351929
763 763 g, t dbSNP:200514878
770 770 a, c dbSNP:776479933
787 787 g, t dbSNP:577477292
794 794 a, g dbSNP:765165459
804 804 a, g dbSNP:778562920
808 808 a, g dbSNP:146220817
813 813 a, g dbSNP:554846063
815 815 a, g dbSNP:542337864
816 816 a, c dbSNP:777719140
820 820 a, c, t dbSNP:201290700
821 821 a, g dbSNP:774382622
822 822 g, t dbSNP:372946885
832 832 a, g dbSNP:759367396
833 833 a, g dbSNP:74375347
837 837 c, t dbSNP:776567252
842 842 a, c dbSNP:762027127
845 845 c, t dbSNP:765384989
850 850 g, t dbSNP:750810564
851 851 c, t dbSNP:142423874
854 854 c, g, t dbSNP:79095986
855 855 a, g dbSNP:755430626
857 857 a, g, t dbSNP:780823917
860 860 -, gtgggt dbSNP:745383395
860 860 c, t dbSNP:190517526
861 861 a, g dbSNP:367797052
862 862 a, t dbSNP:748913627
864 864 g, t dbSNP:371497237
870 870 a, g dbSNP:778556850
875 875 a, c, t dbSNP:199858417
876 876 a, g dbSNP:1139971
878 878 c, g, t dbSNP:150062713
882 882 c, t dbSNP:754907833
890 890 g, t dbSNP:777837925
897 897 c, t dbSNP:749272629
899 899 c, g dbSNP:74885383
903 903 a, g dbSNP:778967682
914 914 c, t dbSNP:746076379
915 915 c, t dbSNP:201405906
916 916 a, g dbSNP:545998821
920 920 c, t dbSNP:761266322
921 921 a, c, g dbSNP:142351354
923 923 c, g dbSNP:7107335
929 929 a, c, g, t dbSNP:201650491
930 930 a, g dbSNP:766230830
938 938 c, t dbSNP:751291553
939 939 a, g dbSNP:754925345
945 945 c, g dbSNP:781061734
948 948 c, t dbSNP:147912305
949 949 c, t dbSNP:201671713
950 950 c, g dbSNP:148874083
955 955 a, g dbSNP:773237459
959 959 a, g dbSNP:772351881
960 960 a, g dbSNP:780577629
965 965 c, g dbSNP:747327385
971 971 c, t dbSNP:376795165
978 978 c, t dbSNP:777044032
980 980 c, t dbSNP:370925717
984 984 a, g, t dbSNP:376175476
985 985 c, t dbSNP:762518913
989 989 a, g dbSNP:765960487
990 990 c, g, t dbSNP:751381541
993 993 c, g dbSNP:767273646
996 996 a, g dbSNP:752630534
1003 1003 a, g dbSNP:756070640
1010 1010 a, c dbSNP:778859812
1024 1024 c, t dbSNP:547161316
1047 1047 c, g dbSNP:529985982
1075 1075 cg, tc dbSNP:386753094
1075 1075 c, t dbSNP:4755266
1076 1076 c, g dbSNP:77781241
1091 1091 a, g dbSNP:569628883
1092 1092 a, g dbSNP:371456705
1176 1176 a, g dbSNP:536686665
1177 1177 c, t dbSNP:374896443
1191 1191 c, t dbSNP:71490073
1193 1193 c, t dbSNP:555408480
1206 1206 a, c dbSNP:116250454
1222 1222 a, g dbSNP:536180457
1226 1226 c, t dbSNP:546842038
1233 1233 a, g dbSNP:554375084
1243 1243 c, t dbSNP:193127050
1283 1283 a, g dbSNP:778679874
1293 1293 a, c dbSNP:539902023
1297 1297 a, g dbSNP:745846614
1308 1308 a, g dbSNP:1063031
1315 1315 c, g dbSNP:12225877
1318 1318 a, g dbSNP:576375816
1321 1321 c, t dbSNP:543364816
1322 1322 a, g dbSNP:572260612
1324 1324 a, g dbSNP:538794913
1331 1331 c, g dbSNP:759814577
1333 1333 c, t dbSNP:540960667
1334 1334 c, g dbSNP:371743094
1341 1341 a, g dbSNP:533024020
1362 1362 a, g dbSNP:113805595
1367 1367 a, g dbSNP:559005209
1373 1373 a, g dbSNP:112667982
1378 1378 a, g dbSNP:563192606
1391 1391 c, t dbSNP:530636550
1392 1392 a, g dbSNP:538247800
1395 1395 a, c dbSNP:6485552
1400 1400 a, c dbSNP:370894393
1411 1411 c, t dbSNP:548974243
1422 1422 c, t dbSNP:374579809
1423 1423 a, g dbSNP:145762348
1426 1426 c, t dbSNP:536141761
1437 1437 c, t dbSNP:780025065
1446 1446 a, c dbSNP:367590200
1455 1455 a, g dbSNP:111384781
1459 1459 a, g dbSNP:143013555
1461 1461 a, g dbSNP:539686462
1466 1466 c, t dbSNP:558340117
1467 1467 a, g dbSNP:576431366
1470 1470 c, t dbSNP:537163605
1471 1471 a, g dbSNP:555325573
1490 1490 c, t dbSNP:573724569
1521 1521 a, c dbSNP:540921974
1522 1522 c, t dbSNP:559427355
1523 1523 a, g dbSNP:577875518
1528 1528 c, t dbSNP:190156561
1529 1529 a, g dbSNP:563522625
1540 1540 c, t dbSNP:761198379
1556 1556 c, t dbSNP:530589270
1558 1558 -, c dbSNP:374072292
1567 1567 -, gcctgg dbSNP:528703148
1575 1575 c, g dbSNP:549135777
1576 1576 g, t dbSNP:560848356
1590 1590 c, t dbSNP:528140057
1597 1597 -, aaa dbSNP:752801473
1597 1597 -, a dbSNP:561662297
1598 1598 -, aa dbSNP:775954230
1615 1615 -, aa dbSNP:398045166
1621 1621 -, aa dbSNP:397848957
1623 1623 c, t dbSNP:780140130
1639 1639 -, cg dbSNP:376197276
1639 1639 a, g dbSNP:146156139
1703 1703 a, c dbSNP:75976933
1728 1728 c, t dbSNP:566202585
1743 1743 a, c dbSNP:769396753
1767 1767 c, t dbSNP:540147029
1768 1768 c, t dbSNP:551648141
1780 1780 g, t dbSNP:762435369
1861 1861 c, t dbSNP:753710390
1862 1862 a, g dbSNP:570264504
1867 1867 a, g dbSNP:115328824
1888 1888 -, g dbSNP:34626122
1888 1888 g, t dbSNP:553986435
1898 1898 a, g dbSNP:754710250
1902 1902 c, g dbSNP:555485604
1905 1905 a, g dbSNP:140021217
1919 1919 c, g dbSNP:778723164
1934 1934 a, g dbSNP:111689312
1954 1954 a, g dbSNP:115940873
1969 1969 c, t dbSNP:763484969
1989 1989 a, g dbSNP:577833901
2004 2004 c, t dbSNP:546035719
2005 2005 a, g dbSNP:556863887
2017 2017 a, g dbSNP:772217498
2031 2031 a, g dbSNP:575540698
2055 2055 a, g dbSNP:181190575
2080 2080 g, t dbSNP:561007915
2105 2105 g, t dbSNP:528204027
2112 2112 g, t dbSNP:56028963
2123 2123 a, g dbSNP:564910931

Target ORF information:

RefSeq Version XM_006718223
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens CD82 molecule (CD82), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006718223

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGCTCAG CCTGTATCAA AGTCACCAAA TACTTTCTCT TCCTCTTCAA CTTGATCTTC 
TTTATCCTGG GCGCAGTGAT CCTGGGCTTC GGGGTGTGGA TCCTGGCCGA CAAGAGCAGT
TTCATCTCTG TCCTGCAAAC CTCCTCCAGC TCGCTTAGGA TGGGGGCCTA TGTCTTCATC
GGCGTGGGGG CAGTCACTAT GCTCATGGGC TTCCTGGGCT GCATCGGCGC CGTCAACGAG
GTCCGCTGCC TGCTGGGGCT GTACTTTGCT TTCCTGCTCC TGATCCTCAT TGCCCAGGTG
ACGGCCGGGG CCCTCTTCTA CTTCAACATG GGCAAGCTGA AGCAGGAGAT GGGCGGCATC
GTGACTGAGC TCATTCGAGA CTACAACAGC AGTCGCGAGG ACAGCCTGCA GGATGCCTGG
GACTACGTGC AGGCTCAGGT GAAGTGCTGC GGCTGGGTCA GCTTCTACAA CTGGACAGAC
AACGCTGAGC TCATGAATCG CCCTGAGGTC ACCTACCCCT GTTCCTGCGA AGTCAAGGGG
GAAGAGGACA ACAGCCTTTC TGTGAGGAAG GGCTTCTGCG AGGCCCCCGG CAACAGGACC
CAGAGTGGCA ACCACCCTGA GGACTGGCCT GTGTACCAGG AGGGCTGCAT GGAGAAGGTG
CAGGCGTGGC TGCAGGAGAA CCTGGGCATC ATCCTCGGCG TGGGCGTGGG TGTGGCCATC
ATCGAGCTCC TGGGGATGGT CCTGTCCATC TGCTTGTGCC GGCACGTCCA TTCCGAAGAC
TACAGCAAGG TCCCCAAGTA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58546
Accession Version XM_011520067.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 711bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product CD82 antigen isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGTCAGAGT TGGAGACGGC GTGTGGAAAG CCCATGAGGC ACAAAACCTC CTCCAGCTCG 
CTTAGGATGG GGGCCTATGT CTTCATCGGC GTGGGGGCAG TCACTATGCT CATGGGCTTC
CTGGGCTGCA TCGGCGCCGT CAACGAGGTC CGCTGCCTGC TGGGGCTGTA CTTTGCTTTC
CTGCTCCTGA TCCTCATTGC CCAGGTGACG GCCGGGGCCC TCTTCTACTT CAACATGGGC
AAGCTGAAGC AGGAGATGGG CGGCATCGTG ACTGAGCTCA TTCGAGACTA CAACAGCAGT
CGCGAGGACA GCCTGCAGGA TGCCTGGGAC TACGTGCAGG CTCAGGTGAA GTGCTGCGGC
TGGGTCAGCT TCTACAACTG GACAGACAAC GCTGAGCTCA TGAATCGCCC TGAGGTCACC
TACCCCTGTT CCTGCGAAGT CAAGGGGGAA GAGGACAACA GCCTTTCTGT GAGGAAGGGC
TTCTGCGAGG CCCCCGGCAA CAGGACCCAG AGTGGCAACC ACCCTGAGGA CTGGCCTGTG
TACCAGGAGG GCTGCATGGA GAAGGTGCAG GCGTGGCTGC AGGAGAACCT GGGCATCATC
CTCGGCGTGG GCGTGGGTGT GGCCATCATC GAGCTCCTGG GGATGGTCCT GTCCATCTGC
TTGTGCCGGC ACGTCCATTC CGAAGACTAC AGCAAGGTCC CCAAGTACTG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011518369.1
CDS25..735
Misc Feature(1)70..699(+)
Misc Feature(2)247..618(+)
Misc Feature(3)253..372(+)
Translation
Position Chain Variation Link
15 15 a, g dbSNP:569132689
55 55 c, t dbSNP:536315686
74 74 c, t dbSNP:752840514
83 83 a, c dbSNP:139889503
84 84 a, g dbSNP:777966335
87 87 c, t dbSNP:374050393
90 90 g, t dbSNP:201294710
100 100 c, t dbSNP:371929631
102 102 c, t dbSNP:143270517
103 103 a, g dbSNP:375078073
111 111 c, g, t dbSNP:146594387
114 114 a, c, g dbSNP:372931321
117 117 a, g dbSNP:34170190
129 129 c, t dbSNP:375797501
130 130 a, g dbSNP:769486957
136 136 a, c, t dbSNP:562318494
144 144 c, t dbSNP:149844780
152 152 g, t dbSNP:774467192
156 156 c, g dbSNP:759521623
157 157 a, g, t dbSNP:369269460
162 162 c, t dbSNP:757425084
167 167 a, g dbSNP:765440814
169 169 a, g dbSNP:145881169
175 175 c, g dbSNP:758720179
176 176 a, g dbSNP:148925723
181 181 c, t dbSNP:747565395
183 183 g, t dbSNP:370728156
189 189 g, t dbSNP:35348953
225 225 a, c dbSNP:766789029
227 227 a, c dbSNP:751917502
235 235 g, t dbSNP:756306249
236 236 a, c dbSNP:781684485
238 238 a, g dbSNP:574662941
241 241 a, g dbSNP:755612375
249 249 c, t dbSNP:780007862
257 257 a, g dbSNP:546228845
260 260 c, t dbSNP:541705376
279 279 a, g dbSNP:2303865
281 281 c, t dbSNP:762967350
282 282 a, g dbSNP:374008730
283 283 a, g dbSNP:774518909
285 285 c, t dbSNP:883752
286 286 a, g dbSNP:368613608
289 289 a, g dbSNP:752996542
291 291 c, t dbSNP:761244897
294 294 a, g dbSNP:2303864
301 301 c, t dbSNP:138793220
308 308 a, g dbSNP:756787839
310 310 a, g dbSNP:764806308
313 313 -, acaa dbSNP:755032584
314 314 a, g dbSNP:750138207
315 315 c, t dbSNP:757975489
316 316 a, g dbSNP:779968308
318 318 c, t dbSNP:746715629
323 323 g, t dbSNP:754923253
325 325 c, t dbSNP:142819851
326 326 a, g, t dbSNP:117157354
327 327 c, t dbSNP:774394673
328 328 a, g, t dbSNP:746166676
330 330 g, t dbSNP:11541051
356 356 a, c dbSNP:775933913
357 357 c, t dbSNP:573753161
358 358 a, g dbSNP:200277144
360 360 g, t dbSNP:781269208
361 361 c, g dbSNP:745695307
364 364 g, t dbSNP:777316375
367 367 -, aggtg dbSNP:778999473
368 368 a, g dbSNP:762386509
371 371 a, t dbSNP:751215015
381 381 a, c, t dbSNP:150371864
382 382 a, g dbSNP:752419097
388 388 a, g dbSNP:138109186
389 389 g, t dbSNP:756053924
390 390 c, t dbSNP:777519636
393 393 c, t dbSNP:750546226
399 399 c, t dbSNP:758448103
401 401 a, g dbSNP:780157672
408 408 a, c dbSNP:747290721
413 413 a, g dbSNP:373060622
414 414 c, t dbSNP:200149429
430 430 c, t dbSNP:149456679
431 431 a, g dbSNP:143845294
432 432 c, t dbSNP:541161774
434 434 c, t dbSNP:377503391
441 441 c, t dbSNP:267602880
449 449 c, t dbSNP:770399013
455 455 c, t dbSNP:773901832
459 459 a, c, t dbSNP:139816598
460 460 a, g dbSNP:370975589
466 466 a, g dbSNP:760418741
469 469 a, g dbSNP:763918347
470 470 a, g dbSNP:763742776
471 471 a, g dbSNP:753768500
472 472 a, g dbSNP:757203164
484 484 a, g dbSNP:780063147
485 485 a, g dbSNP:751624745
489 489 c, t dbSNP:766762693
491 491 c, t dbSNP:755265941
496 496 -, agga dbSNP:758151345
500 500 a, g dbSNP:781515941
503 503 a, g dbSNP:748555757
510 510 c, t dbSNP:141597594
511 511 a, g dbSNP:200920132
515 515 c, g, t dbSNP:749820676
519 519 a, c, t dbSNP:773861588
520 520 a, g dbSNP:771739391
530 530 c, t dbSNP:775162522
534 534 g, t dbSNP:760351929
539 539 g, t dbSNP:200514878
546 546 a, c dbSNP:776479933
563 563 g, t dbSNP:577477292
570 570 a, g dbSNP:765165459
580 580 a, g dbSNP:778562920
584 584 a, g dbSNP:146220817
589 589 a, g dbSNP:554846063
591 591 a, g dbSNP:542337864
592 592 a, c dbSNP:777719140
596 596 a, c, t dbSNP:201290700
597 597 a, g dbSNP:774382622
598 598 g, t dbSNP:372946885
608 608 a, g dbSNP:759367396
609 609 a, g dbSNP:74375347
613 613 c, t dbSNP:776567252
618 618 a, c dbSNP:762027127
621 621 c, t dbSNP:765384989
626 626 g, t dbSNP:750810564
627 627 c, t dbSNP:142423874
630 630 c, g, t dbSNP:79095986
631 631 a, g dbSNP:755430626
633 633 a, g, t dbSNP:780823917
636 636 -, gtgggt dbSNP:745383395
636 636 c, t dbSNP:190517526
637 637 a, g dbSNP:367797052
638 638 a, t dbSNP:748913627
640 640 g, t dbSNP:371497237
646 646 a, g dbSNP:778556850
651 651 a, c, t dbSNP:199858417
652 652 a, g dbSNP:1139971
654 654 c, g, t dbSNP:150062713
658 658 c, t dbSNP:754907833
666 666 g, t dbSNP:777837925
673 673 c, t dbSNP:749272629
675 675 c, g dbSNP:74885383
679 679 a, g dbSNP:778967682
690 690 c, t dbSNP:746076379
691 691 c, t dbSNP:201405906
692 692 a, g dbSNP:545998821
696 696 c, t dbSNP:761266322
697 697 a, c, g dbSNP:142351354
699 699 c, g dbSNP:7107335
705 705 a, c, g, t dbSNP:201650491
706 706 a, g dbSNP:766230830
714 714 c, t dbSNP:751291553
715 715 a, g dbSNP:754925345
721 721 c, g dbSNP:781061734
724 724 c, t dbSNP:147912305
725 725 c, t dbSNP:201671713
726 726 c, g dbSNP:148874083
731 731 a, g dbSNP:773237459
735 735 a, g dbSNP:772351881
736 736 a, g dbSNP:780577629
741 741 c, g dbSNP:747327385
747 747 c, t dbSNP:376795165
754 754 c, t dbSNP:777044032
756 756 c, t dbSNP:370925717
760 760 a, g, t dbSNP:376175476
761 761 c, t dbSNP:762518913
765 765 a, g dbSNP:765960487
766 766 c, g, t dbSNP:751381541
769 769 c, g dbSNP:767273646
772 772 a, g dbSNP:752630534
779 779 a, g dbSNP:756070640
786 786 a, c dbSNP:778859812
800 800 c, t dbSNP:547161316
823 823 c, g dbSNP:529985982
851 851 cg, tc dbSNP:386753094
851 851 c, t dbSNP:4755266
852 852 c, g dbSNP:77781241
867 867 a, g dbSNP:569628883
868 868 a, g dbSNP:371456705
952 952 a, g dbSNP:536686665
953 953 c, t dbSNP:374896443
967 967 c, t dbSNP:71490073
969 969 c, t dbSNP:555408480
982 982 a, c dbSNP:116250454
998 998 a, g dbSNP:536180457
1002 1002 c, t dbSNP:546842038
1009 1009 a, g dbSNP:554375084
1019 1019 c, t dbSNP:193127050
1059 1059 a, g dbSNP:778679874
1069 1069 a, c dbSNP:539902023
1073 1073 a, g dbSNP:745846614
1084 1084 a, g dbSNP:1063031
1091 1091 c, g dbSNP:12225877
1094 1094 a, g dbSNP:576375816
1097 1097 c, t dbSNP:543364816
1098 1098 a, g dbSNP:572260612
1100 1100 a, g dbSNP:538794913
1107 1107 c, g dbSNP:759814577
1109 1109 c, t dbSNP:540960667
1110 1110 c, g dbSNP:371743094
1117 1117 a, g dbSNP:533024020
1138 1138 a, g dbSNP:113805595
1143 1143 a, g dbSNP:559005209
1149 1149 a, g dbSNP:112667982
1154 1154 a, g dbSNP:563192606
1167 1167 c, t dbSNP:530636550
1168 1168 a, g dbSNP:538247800
1171 1171 a, c dbSNP:6485552
1176 1176 a, c dbSNP:370894393
1187 1187 c, t dbSNP:548974243
1198 1198 c, t dbSNP:374579809
1199 1199 a, g dbSNP:145762348
1202 1202 c, t dbSNP:536141761
1213 1213 c, t dbSNP:780025065
1222 1222 a, c dbSNP:367590200
1231 1231 a, g dbSNP:111384781
1235 1235 a, g dbSNP:143013555
1237 1237 a, g dbSNP:539686462
1242 1242 c, t dbSNP:558340117
1243 1243 a, g dbSNP:576431366
1246 1246 c, t dbSNP:537163605
1247 1247 a, g dbSNP:555325573
1266 1266 c, t dbSNP:573724569
1297 1297 a, c dbSNP:540921974
1298 1298 c, t dbSNP:559427355
1299 1299 a, g dbSNP:577875518
1304 1304 c, t dbSNP:190156561
1305 1305 a, g dbSNP:563522625
1316 1316 c, t dbSNP:761198379
1332 1332 c, t dbSNP:530589270
1334 1334 -, c dbSNP:374072292
1343 1343 -, gcctgg dbSNP:528703148
1351 1351 c, g dbSNP:549135777
1352 1352 g, t dbSNP:560848356
1366 1366 c, t dbSNP:528140057
1373 1373 -, aaa dbSNP:752801473
1373 1373 -, a dbSNP:561662297
1374 1374 -, aa dbSNP:775954230
1391 1391 -, aa dbSNP:398045166
1397 1397 -, aa dbSNP:397848957
1399 1399 c, t dbSNP:780140130
1415 1415 -, cg dbSNP:376197276
1415 1415 a, g dbSNP:146156139
1479 1479 a, c dbSNP:75976933
1504 1504 c, t dbSNP:566202585
1519 1519 a, c dbSNP:769396753
1543 1543 c, t dbSNP:540147029
1544 1544 c, t dbSNP:551648141
1556 1556 g, t dbSNP:762435369
1637 1637 c, t dbSNP:753710390
1638 1638 a, g dbSNP:570264504
1643 1643 a, g dbSNP:115328824
1664 1664 -, g dbSNP:34626122
1664 1664 g, t dbSNP:553986435
1674 1674 a, g dbSNP:754710250
1678 1678 c, g dbSNP:555485604
1681 1681 a, g dbSNP:140021217
1695 1695 c, g dbSNP:778723164
1710 1710 a, g dbSNP:111689312
1730 1730 a, g dbSNP:115940873
1745 1745 c, t dbSNP:763484969
1765 1765 a, g dbSNP:577833901
1780 1780 c, t dbSNP:546035719
1781 1781 a, g dbSNP:556863887
1793 1793 a, g dbSNP:772217498
1807 1807 a, g dbSNP:575540698
1831 1831 a, g dbSNP:181190575
1856 1856 g, t dbSNP:561007915
1881 1881 g, t dbSNP:528204027
1888 1888 g, t dbSNP:56028963
1899 1899 a, g dbSNP:564910931

Target ORF information:

RefSeq Version XM_011520067
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens CD82 molecule (CD82), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011520067

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGTCAGAGT TGGAGACGGC GTGTGGAAAG CCCATGAGGC ACAAAACCTC CTCCAGCTCG 
CTTAGGATGG GGGCCTATGT CTTCATCGGC GTGGGGGCAG TCACTATGCT CATGGGCTTC
CTGGGCTGCA TCGGCGCCGT CAACGAGGTC CGCTGCCTGC TGGGGCTGTA CTTTGCTTTC
CTGCTCCTGA TCCTCATTGC CCAGGTGACG GCCGGGGCCC TCTTCTACTT CAACATGGGC
AAGCTGAAGC AGGAGATGGG CGGCATCGTG ACTGAGCTCA TTCGAGACTA CAACAGCAGT
CGCGAGGACA GCCTGCAGGA TGCCTGGGAC TACGTGCAGG CTCAGGTGAA GTGCTGCGGC
TGGGTCAGCT TCTACAACTG GACAGACAAC GCTGAGCTCA TGAATCGCCC TGAGGTCACC
TACCCCTGTT CCTGCGAAGT CAAGGGGGAA GAGGACAACA GCCTTTCTGT GAGGAAGGGC
TTCTGCGAGG CCCCCGGCAA CAGGACCCAG AGTGGCAACC ACCCTGAGGA CTGGCCTGTG
TACCAGGAGG GCTGCATGGA GAAGGTGCAG GCGTGGCTGC AGGAGAACCT GGGCATCATC
CTCGGCGTGG GCGTGGGTGT GGCCATCATC GAGCTCCTGG GGATGGTCCT GTCCATCTGC
TTGTGCCGGC ACGTCCATTC CGAAGACTAC AGCAAGGTCC CCAAGTACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26932
Accession Version NM_002231.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 804bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product CD82 antigen isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB127804.1, U20770.1 and AC010768.10. This sequence is a reference standard in the RefSeqGene project. On Jun 15, 2005 this sequence version replaced gi:13259537. Summary: This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225708.1, AK225410.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGCTCAG CCTGTATCAA AGTCACCAAA TACTTTCTCT TCCTCTTCAA CTTGATCTTC 
TTTATCCTGG GCGCAGTGAT CCTGGGCTTC GGGGTGTGGA TCCTGGCCGA CAAGAGCAGT
TTCATCTCTG TCCTGCAAAC CTCCTCCAGC TCGCTTAGGA TGGGGGCCTA TGTCTTCATC
GGCGTGGGGG CAGTCACTAT GCTCATGGGC TTCCTGGGCT GCATCGGCGC CGTCAACGAG
GTCCGCTGCC TGCTGGGGCT GTACTTTGCT TTCCTGCTCC TGATCCTCAT TGCCCAGGTG
ACGGCCGGGG CCCTCTTCTA CTTCAACATG GGCAAGCTGA AGCAGGAGAT GGGCGGCATC
GTGACTGAGC TCATTCGAGA CTACAACAGC AGTCGCGAGG ACAGCCTGCA GGATGCCTGG
GACTACGTGC AGGCTCAGGT GAAGTGCTGC GGCTGGGTCA GCTTCTACAA CTGGACAGAC
AACGCTGAGC TCATGAATCG CCCTGAGGTC ACCTACCCCT GTTCCTGCGA AGTCAAGGGG
GAAGAGGACA ACAGCCTTTC TGTGAGGAAG GGCTTCTGCG AGGCCCCCGG CAACAGGACC
CAGAGTGGCA ACCACCCTGA GGACTGGCCT GTGTACCAGG AGGGCTGCAT GGAGAAGGTG
CAGGCGTGGC TGCAGGAGAA CCTGGGCATC ATCCTCGGCG TGGGCGTGGG TGTGGCCATC
ATCGAGCTCC TGGGGATGGT CCTGTCCATC TGCTTGTGCC GGCACGTCCA TTCCGAAGAC
TACAGCAAGG TCCCCAAGTA CTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_002222.1
CDS249..1052
Misc Feature(1)54..56(+)
Misc Feature(2)282..344(+)
Misc Feature(3)318..1016(+)
Misc Feature(4)408..464(+)
Misc Feature(5)498..578(+)
Misc Feature(6)564..935(+)
Misc Feature(7)570..689(+)
Misc Feature(8)633..635(+)
Misc Feature(9)717..719(+)
Misc Feature(10)840..842(+)
Misc Feature(11)933..998(+)
Exon (1)1..146
Gene:CD82
Gene Synonym:
Exon (2)147..228
Gene:CD82
Gene Synonym:
Exon (3)229..311
Gene:CD82
Gene Synonym:
Exon (4)312..384
Gene:CD82
Gene Synonym:
Exon (5)385..509
Gene:CD82
Gene Synonym:
Exon (6)510..584
Gene:CD82
Gene Synonym:
Exon (7)585..686
Gene:CD82
Gene Synonym:
Exon (8)687..890
Gene:CD82
Gene Synonym:
Exon (9)891..974
Gene:CD82
Gene Synonym:
Exon (10)975..1715
Gene:CD82
Gene Synonym:
Translation
Position Chain Variation Link
14 14 c, t dbSNP:541007379
19 19 c, g dbSNP:548078967
24 24 c, t dbSNP:576592622
25 25 a, c dbSNP:544417680
34 34 a, g dbSNP:766242947
47 47 a, c dbSNP:562398194
64 64 c, t dbSNP:182579675
80 80 a, g dbSNP:11541048
117 117 a, g dbSNP:564400097
122 122 a, g dbSNP:753631989
131 131 a, g dbSNP:547873874
159 159 a, t dbSNP:754565162
161 161 c, t dbSNP:760477547
170 170 c, t dbSNP:552512734
171 171 a, g dbSNP:747933620
176 176 a, g dbSNP:764119634
194 194 g, t dbSNP:532951527
213 213 a, g dbSNP:769734246
221 221 c, t dbSNP:776219159
225 225 g, t dbSNP:11541050
232 232 c, g dbSNP:777055402
236 236 c, t dbSNP:762212914
245 245 c, t dbSNP:200238062
246 246 a, g dbSNP:773819071
248 248 a, g dbSNP:763522693
249 249 a, g, t dbSNP:765963603
252 252 a, g dbSNP:111718702
253 253 a, g dbSNP:767017762
256 256 c, t dbSNP:752510747
285 285 a, c dbSNP:761257124
287 287 a, c dbSNP:777513108
290 290 a, c dbSNP:749268925
300 300 c, t dbSNP:752146609
305 305 a, c dbSNP:372259856
314 314 c, t dbSNP:774679121
318 318 a, g dbSNP:760055528
320 320 c, t dbSNP:535717592
321 321 a, g dbSNP:774964064
323 323 a, g dbSNP:760383801
325 325 c, t dbSNP:749539429
334 334 c, g dbSNP:763666572
339 339 a, g dbSNP:554121820
341 341 a, g dbSNP:761468393
343 343 c, t dbSNP:148396643
352 352 c, t dbSNP:377535419
356 356 c, t dbSNP:138833231
357 357 a, g dbSNP:758337930
367 367 a, c, g dbSNP:781480823
372 372 a, g dbSNP:756411683
378 378 a, g dbSNP:778085878
384 384 c, t dbSNP:150533529
391 391 c, t dbSNP:752840514
400 400 a, c dbSNP:139889503
401 401 a, g dbSNP:777966335
404 404 c, t dbSNP:374050393
407 407 g, t dbSNP:201294710
417 417 c, t dbSNP:371929631
419 419 c, t dbSNP:143270517
420 420 a, g dbSNP:375078073
428 428 c, g, t dbSNP:146594387
431 431 a, c, g dbSNP:372931321
434 434 a, g dbSNP:34170190
446 446 c, t dbSNP:375797501
447 447 a, g dbSNP:769486957
453 453 a, c, t dbSNP:562318494
461 461 c, t dbSNP:149844780
469 469 g, t dbSNP:774467192
473 473 c, g dbSNP:759521623
474 474 a, g, t dbSNP:369269460
479 479 c, t dbSNP:757425084
484 484 a, g dbSNP:765440814
486 486 a, g dbSNP:145881169
492 492 c, g dbSNP:758720179
493 493 a, g dbSNP:148925723
498 498 c, t dbSNP:747565395
500 500 g, t dbSNP:370728156
506 506 g, t dbSNP:35348953
542 542 a, c dbSNP:766789029
544 544 a, c dbSNP:751917502
552 552 g, t dbSNP:756306249
553 553 a, c dbSNP:781684485
555 555 a, g dbSNP:574662941
558 558 a, g dbSNP:755612375
566 566 c, t dbSNP:780007862
574 574 a, g dbSNP:546228845
577 577 c, t dbSNP:541705376
596 596 a, g dbSNP:2303865
598 598 c, t dbSNP:762967350
599 599 a, g dbSNP:374008730
600 600 a, g dbSNP:774518909
602 602 c, t dbSNP:883752
603 603 a, g dbSNP:368613608
606 606 a, g dbSNP:752996542
608 608 c, t dbSNP:761244897
611 611 a, g dbSNP:2303864
618 618 c, t dbSNP:138793220
625 625 a, g dbSNP:756787839
627 627 a, g dbSNP:764806308
630 630 -, acaa dbSNP:755032584
631 631 a, g dbSNP:750138207
632 632 c, t dbSNP:757975489
633 633 a, g dbSNP:779968308
635 635 c, t dbSNP:746715629
640 640 g, t dbSNP:754923253
642 642 c, t dbSNP:142819851
643 643 a, g, t dbSNP:117157354
644 644 c, t dbSNP:774394673
645 645 a, g, t dbSNP:746166676
647 647 g, t dbSNP:11541051
673 673 a, c dbSNP:775933913
674 674 c, t dbSNP:573753161
675 675 a, g dbSNP:200277144
677 677 g, t dbSNP:781269208
678 678 c, g dbSNP:745695307
681 681 g, t dbSNP:777316375
684 684 -, aggtg dbSNP:778999473
685 685 a, g dbSNP:762386509
688 688 a, t dbSNP:751215015
698 698 a, c, t dbSNP:150371864
699 699 a, g dbSNP:752419097
705 705 a, g dbSNP:138109186
706 706 g, t dbSNP:756053924
707 707 c, t dbSNP:777519636
710 710 c, t dbSNP:750546226
716 716 c, t dbSNP:758448103
718 718 a, g dbSNP:780157672
725 725 a, c dbSNP:747290721
730 730 a, g dbSNP:373060622
731 731 c, t dbSNP:200149429
747 747 c, t dbSNP:149456679
748 748 a, g dbSNP:143845294
749 749 c, t dbSNP:541161774
751 751 c, t dbSNP:377503391
758 758 c, t dbSNP:267602880
766 766 c, t dbSNP:770399013
772 772 c, t dbSNP:773901832
776 776 a, c, t dbSNP:139816598
777 777 a, g dbSNP:370975589
783 783 a, g dbSNP:760418741
786 786 a, g dbSNP:763918347
787 787 a, g dbSNP:763742776
788 788 a, g dbSNP:753768500
789 789 a, g dbSNP:757203164
801 801 a, g dbSNP:780063147
802 802 a, g dbSNP:751624745
806 806 c, t dbSNP:766762693
808 808 c, t dbSNP:755265941
813 813 -, agga dbSNP:758151345
817 817 a, g dbSNP:781515941
820 820 a, g dbSNP:748555757
827 827 c, t dbSNP:141597594
828 828 a, g dbSNP:200920132
832 832 c, g, t dbSNP:749820676
836 836 a, c, t dbSNP:773861588
837 837 a, g dbSNP:771739391
847 847 c, t dbSNP:775162522
851 851 g, t dbSNP:760351929
856 856 g, t dbSNP:200514878
863 863 a, c dbSNP:776479933
880 880 g, t dbSNP:577477292
887 887 a, g dbSNP:765165459
897 897 a, g dbSNP:778562920
901 901 a, g dbSNP:146220817
906 906 a, g dbSNP:554846063
908 908 a, g dbSNP:542337864
909 909 a, c dbSNP:777719140
913 913 a, c, t dbSNP:201290700
914 914 a, g dbSNP:774382622
915 915 g, t dbSNP:372946885
925 925 a, g dbSNP:759367396
926 926 a, g dbSNP:74375347
930 930 c, t dbSNP:776567252
935 935 a, c dbSNP:762027127
938 938 c, t dbSNP:765384989
943 943 g, t dbSNP:750810564
944 944 c, t dbSNP:142423874
947 947 c, g, t dbSNP:79095986
948 948 a, g dbSNP:755430626
950 950 a, g, t dbSNP:780823917
953 953 -, gtgggt dbSNP:745383395
953 953 c, t dbSNP:190517526
954 954 a, g dbSNP:367797052
955 955 a, t dbSNP:748913627
957 957 g, t dbSNP:371497237
963 963 a, g dbSNP:778556850
968 968 a, c, t dbSNP:199858417
969 969 a, g dbSNP:1139971
971 971 c, g, t dbSNP:150062713
975 975 c, t dbSNP:754907833
983 983 g, t dbSNP:777837925
990 990 c, t dbSNP:749272629
992 992 c, g dbSNP:74885383
996 996 a, g dbSNP:778967682
1007 1007 c, t dbSNP:746076379
1008 1008 c, t dbSNP:201405906
1009 1009 a, g dbSNP:545998821
1013 1013 c, t dbSNP:761266322
1014 1014 a, c, g dbSNP:142351354
1016 1016 c, g dbSNP:7107335
1022 1022 a, c, g, t dbSNP:201650491
1023 1023 a, g dbSNP:766230830
1031 1031 c, t dbSNP:751291553
1032 1032 a, g dbSNP:754925345
1038 1038 c, g dbSNP:781061734
1041 1041 c, t dbSNP:147912305
1042 1042 c, t dbSNP:201671713
1043 1043 c, g dbSNP:148874083
1048 1048 a, g dbSNP:773237459
1052 1052 a, g dbSNP:772351881
1053 1053 a, g dbSNP:780577629
1058 1058 c, g dbSNP:747327385
1064 1064 c, t dbSNP:376795165
1071 1071 c, t dbSNP:777044032
1073 1073 c, t dbSNP:370925717
1077 1077 a, g, t dbSNP:376175476
1078 1078 c, t dbSNP:762518913
1082 1082 a, g dbSNP:765960487
1083 1083 c, g, t dbSNP:751381541
1086 1086 c, g dbSNP:767273646
1089 1089 a, g dbSNP:752630534
1096 1096 a, g dbSNP:756070640
1103 1103 a, c dbSNP:778859812
1117 1117 c, t dbSNP:547161316
1140 1140 c, g dbSNP:529985982
1168 1168 cg, tc dbSNP:386753094
1168 1168 c, t dbSNP:4755266
1169 1169 c, g dbSNP:77781241
1184 1184 a, g dbSNP:569628883
1185 1185 a, g dbSNP:371456705
1269 1269 a, g dbSNP:536686665
1270 1270 c, t dbSNP:374896443
1284 1284 c, t dbSNP:71490073
1286 1286 c, t dbSNP:555408480
1299 1299 a, c dbSNP:116250454
1315 1315 a, g dbSNP:536180457
1319 1319 c, t dbSNP:546842038
1326 1326 a, g dbSNP:554375084
1336 1336 c, t dbSNP:193127050
1376 1376 a, g dbSNP:778679874
1386 1386 a, c dbSNP:539902023
1390 1390 a, g dbSNP:745846614
1401 1401 a, g dbSNP:1063031
1408 1408 c, g dbSNP:12225877
1411 1411 a, g dbSNP:576375816
1414 1414 c, t dbSNP:543364816
1415 1415 a, g dbSNP:572260612
1417 1417 a, g dbSNP:538794913
1424 1424 c, g dbSNP:759814577
1426 1426 c, t dbSNP:540960667
1427 1427 c, g dbSNP:371743094
1434 1434 a, g dbSNP:533024020
1455 1455 a, g dbSNP:113805595
1460 1460 a, g dbSNP:559005209
1466 1466 a, g dbSNP:112667982
1471 1471 a, g dbSNP:563192606
1484 1484 c, t dbSNP:530636550
1485 1485 a, g dbSNP:538247800
1488 1488 a, c dbSNP:6485552
1493 1493 a, c dbSNP:370894393
1504 1504 c, t dbSNP:548974243
1515 1515 c, t dbSNP:374579809
1516 1516 a, g dbSNP:145762348
1519 1519 c, t dbSNP:536141761
1530 1530 c, t dbSNP:780025065
1539 1539 a, c dbSNP:367590200
1548 1548 a, g dbSNP:111384781
1552 1552 a, g dbSNP:143013555
1554 1554 a, g dbSNP:539686462
1559 1559 c, t dbSNP:558340117
1560 1560 a, g dbSNP:576431366
1563 1563 c, t dbSNP:537163605
1564 1564 a, g dbSNP:555325573
1583 1583 c, t dbSNP:573724569
1614 1614 a, c dbSNP:540921974
1615 1615 c, t dbSNP:559427355
1616 1616 a, g dbSNP:577875518
1621 1621 c, t dbSNP:190156561
1622 1622 a, g dbSNP:563522625
1633 1633 c, t dbSNP:761198379
1649 1649 c, t dbSNP:530589270
1651 1651 -, c dbSNP:374072292
1660 1660 -, gcctgg dbSNP:528703148
1668 1668 c, g dbSNP:549135777
1669 1669 g, t dbSNP:560848356
1683 1683 c, t dbSNP:528140057
1690 1690 -, aaa dbSNP:752801473
1690 1690 -, a dbSNP:561662297
1691 1691 -, aa dbSNP:775954230
1708 1708 -, aa dbSNP:398045166
1714 1714 -, aa dbSNP:397848957

Target ORF information:

RefSeq Version NM_002231
Organism Homo sapiens (human)
Definition Homo sapiens CD82 molecule (CD82), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002231

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGCTCAG CCTGTATCAA AGTCACCAAA TACTTTCTCT TCCTCTTCAA CTTGATCTTC 
TTTATCCTGG GCGCAGTGAT CCTGGGCTTC GGGGTGTGGA TCCTGGCCGA CAAGAGCAGT
TTCATCTCTG TCCTGCAAAC CTCCTCCAGC TCGCTTAGGA TGGGGGCCTA TGTCTTCATC
GGCGTGGGGG CAGTCACTAT GCTCATGGGC TTCCTGGGCT GCATCGGCGC CGTCAACGAG
GTCCGCTGCC TGCTGGGGCT GTACTTTGCT TTCCTGCTCC TGATCCTCAT TGCCCAGGTG
ACGGCCGGGG CCCTCTTCTA CTTCAACATG GGCAAGCTGA AGCAGGAGAT GGGCGGCATC
GTGACTGAGC TCATTCGAGA CTACAACAGC AGTCGCGAGG ACAGCCTGCA GGATGCCTGG
GACTACGTGC AGGCTCAGGT GAAGTGCTGC GGCTGGGTCA GCTTCTACAA CTGGACAGAC
AACGCTGAGC TCATGAATCG CCCTGAGGTC ACCTACCCCT GTTCCTGCGA AGTCAAGGGG
GAAGAGGACA ACAGCCTTTC TGTGAGGAAG GGCTTCTGCG AGGCCCCCGG CAACAGGACC
CAGAGTGGCA ACCACCCTGA GGACTGGCCT GTGTACCAGG AGGGCTGCAT GGAGAAGGTG
CAGGCGTGGC TGCAGGAGAA CCTGGGCATC ATCCTCGGCG TGGGCGTGGG TGTGGCCATC
ATCGAGCTCC TGGGGATGGT CCTGTCCATC TGCTTGTGCC GGCACGTCCA TTCCGAAGAC
TACAGCAAGG TCCCCAAGTA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26919
Accession Version NM_001024844.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 729bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product CD82 antigen isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB127804.1, AY303776.1, U20770.1 and AC010768.10. Summary: This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, as compared to variant 1. The encoded isoform (2) thus lacks a segment in the middle region, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY303776.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGGCTCAG CCTGTATCAA AGTCACCAAA TACTTTCTCT TCCTCTTCAA CTTGATCTTC 
TTTATCCTGG GCGCAGTGAT CCTGGGCTTC GGGGTGTGGA TCCTGGCCGA CAAGAGCAGT
TTCATCTCTG TCCTGCAAAC CTCCTCCAGC TCGCTTAGGA TGGGGGCCTA TGTCTTCATC
GGCGTGGGGG CAGTCACTAT GCTCATGGGC TTCCTGGGCT GCATCGGCGC CGTCAACGAG
GTCCGCTGCC TGCTGGGGCT GCTGAAGCAG GAGATGGGCG GCATCGTGAC TGAGCTCATT
CGAGACTACA ACAGCAGTCG CGAGGACAGC CTGCAGGATG CCTGGGACTA CGTGCAGGCT
CAGGTGAAGT GCTGCGGCTG GGTCAGCTTC TACAACTGGA CAGACAACGC TGAGCTCATG
AATCGCCCTG AGGTCACCTA CCCCTGTTCC TGCGAAGTCA AGGGGGAAGA GGACAACAGC
CTTTCTGTGA GGAAGGGCTT CTGCGAGGCC CCCGGCAACA GGACCCAGAG TGGCAACCAC
CCTGAGGACT GGCCTGTGTA CCAGGAGGGC TGCATGGAGA AGGTGCAGGC GTGGCTGCAG
GAGAACCTGG GCATCATCCT CGGCGTGGGC GTGGGTGTGG CCATCATCGA GCTCCTGGGG
ATGGTCCTGT CCATCTGCTT GTGCCGGCAC GTCCATTCCG AAGACTACAG CAAGGTCCCC
AAGTACTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001020015.1
CDS249..977
Misc Feature(1)54..56(+)
Misc Feature(2)318..941(+)
Misc Feature(3)504..860(+)
Misc Feature(4)510..614(+)
Misc Feature(5)558..560(+)
Misc Feature(6)642..644(+)
Misc Feature(7)765..767(+)
Exon (1)1..146
Gene:CD82
Gene Synonym:
Exon (2)147..228
Gene:CD82
Gene Synonym:
Exon (3)229..311
Gene:CD82
Gene Synonym:
Exon (4)312..384
Gene:CD82
Gene Synonym:
Exon (5)385..509
Gene:CD82
Gene Synonym:
Exon (6)510..611
Gene:CD82
Gene Synonym:
Exon (7)612..815
Gene:CD82
Gene Synonym:
Exon (8)816..899
Gene:CD82
Gene Synonym:
Exon (9)900..1640
Gene:CD82
Gene Synonym:
Translation
Position Chain Variation Link
14 14 c, t dbSNP:541007379
19 19 c, g dbSNP:548078967
24 24 c, t dbSNP:576592622
25 25 a, c dbSNP:544417680
34 34 a, g dbSNP:766242947
47 47 a, c dbSNP:562398194
64 64 c, t dbSNP:182579675
80 80 a, g dbSNP:11541048
117 117 a, g dbSNP:564400097
122 122 a, g dbSNP:753631989
131 131 a, g dbSNP:547873874
159 159 a, t dbSNP:754565162
161 161 c, t dbSNP:760477547
170 170 c, t dbSNP:552512734
171 171 a, g dbSNP:747933620
176 176 a, g dbSNP:764119634
194 194 g, t dbSNP:532951527
213 213 a, g dbSNP:769734246
221 221 c, t dbSNP:776219159
225 225 g, t dbSNP:11541050
232 232 c, g dbSNP:777055402
236 236 c, t dbSNP:762212914
245 245 c, t dbSNP:200238062
246 246 a, g dbSNP:773819071
248 248 a, g dbSNP:763522693
249 249 a, g, t dbSNP:765963603
252 252 a, g dbSNP:111718702
253 253 a, g dbSNP:767017762
256 256 c, t dbSNP:752510747
285 285 a, c dbSNP:761257124
287 287 a, c dbSNP:777513108
290 290 a, c dbSNP:749268925
300 300 c, t dbSNP:752146609
305 305 a, c dbSNP:372259856
314 314 c, t dbSNP:774679121
318 318 a, g dbSNP:760055528
320 320 c, t dbSNP:535717592
321 321 a, g dbSNP:774964064
323 323 a, g dbSNP:760383801
325 325 c, t dbSNP:749539429
334 334 c, g dbSNP:763666572
339 339 a, g dbSNP:554121820
341 341 a, g dbSNP:761468393
343 343 c, t dbSNP:148396643
352 352 c, t dbSNP:377535419
356 356 c, t dbSNP:138833231
357 357 a, g dbSNP:758337930
367 367 a, c, g dbSNP:781480823
372 372 a, g dbSNP:756411683
378 378 a, g dbSNP:778085878
384 384 c, t dbSNP:150533529
391 391 c, t dbSNP:752840514
400 400 a, c dbSNP:139889503
401 401 a, g dbSNP:777966335
404 404 c, t dbSNP:374050393
407 407 g, t dbSNP:201294710
417 417 c, t dbSNP:371929631
419 419 c, t dbSNP:143270517
420 420 a, g dbSNP:375078073
428 428 c, g, t dbSNP:146594387
431 431 a, c, g dbSNP:372931321
434 434 a, g dbSNP:34170190
446 446 c, t dbSNP:375797501
447 447 a, g dbSNP:769486957
453 453 a, c, t dbSNP:562318494
461 461 c, t dbSNP:149844780
469 469 g, t dbSNP:774467192
473 473 c, g dbSNP:759521623
474 474 a, g, t dbSNP:369269460
479 479 c, t dbSNP:757425084
484 484 a, g dbSNP:765440814
486 486 a, g dbSNP:145881169
492 492 c, g dbSNP:758720179
493 493 a, g dbSNP:148925723
498 498 c, t dbSNP:747565395
500 500 g, t dbSNP:370728156
506 506 g, t dbSNP:35348953
521 521 a, g dbSNP:2303865
523 523 c, t dbSNP:762967350
524 524 a, g dbSNP:374008730
525 525 a, g dbSNP:774518909
527 527 c, t dbSNP:883752
528 528 a, g dbSNP:368613608
531 531 a, g dbSNP:752996542
533 533 c, t dbSNP:761244897
536 536 a, g dbSNP:2303864
543 543 c, t dbSNP:138793220
550 550 a, g dbSNP:756787839
552 552 a, g dbSNP:764806308
555 555 -, acaa dbSNP:755032584
556 556 a, g dbSNP:750138207
557 557 c, t dbSNP:757975489
558 558 a, g dbSNP:779968308
560 560 c, t dbSNP:746715629
565 565 g, t dbSNP:754923253
567 567 c, t dbSNP:142819851
568 568 a, g, t dbSNP:117157354
569 569 c, t dbSNP:774394673
570 570 a, g, t dbSNP:746166676
572 572 g, t dbSNP:11541051
598 598 a, c dbSNP:775933913
599 599 c, t dbSNP:573753161
600 600 a, g dbSNP:200277144
602 602 g, t dbSNP:781269208
603 603 c, g dbSNP:745695307
606 606 g, t dbSNP:777316375
609 609 -, aggtg dbSNP:778999473
610 610 a, g dbSNP:762386509
613 613 a, t dbSNP:751215015
623 623 a, c, t dbSNP:150371864
624 624 a, g dbSNP:752419097
630 630 a, g dbSNP:138109186
631 631 g, t dbSNP:756053924
632 632 c, t dbSNP:777519636
635 635 c, t dbSNP:750546226
641 641 c, t dbSNP:758448103
643 643 a, g dbSNP:780157672
650 650 a, c dbSNP:747290721
655 655 a, g dbSNP:373060622
656 656 c, t dbSNP:200149429
672 672 c, t dbSNP:149456679
673 673 a, g dbSNP:143845294
674 674 c, t dbSNP:541161774
676 676 c, t dbSNP:377503391
683 683 c, t dbSNP:267602880
691 691 c, t dbSNP:770399013
697 697 c, t dbSNP:773901832
701 701 a, c, t dbSNP:139816598
702 702 a, g dbSNP:370975589
708 708 a, g dbSNP:760418741
711 711 a, g dbSNP:763918347
712 712 a, g dbSNP:763742776
713 713 a, g dbSNP:753768500
714 714 a, g dbSNP:757203164
726 726 a, g dbSNP:780063147
727 727 a, g dbSNP:751624745
731 731 c, t dbSNP:766762693
733 733 c, t dbSNP:755265941
738 738 -, agga dbSNP:758151345
742 742 a, g dbSNP:781515941
745 745 a, g dbSNP:748555757
752 752 c, t dbSNP:141597594
753 753 a, g dbSNP:200920132
757 757 c, g, t dbSNP:749820676
761 761 a, c, t dbSNP:773861588
762 762 a, g dbSNP:771739391
772 772 c, t dbSNP:775162522
776 776 g, t dbSNP:760351929
781 781 g, t dbSNP:200514878
788 788 a, c dbSNP:776479933
805 805 g, t dbSNP:577477292
812 812 a, g dbSNP:765165459
822 822 a, g dbSNP:778562920
826 826 a, g dbSNP:146220817
831 831 a, g dbSNP:554846063
833 833 a, g dbSNP:542337864
834 834 a, c dbSNP:777719140
838 838 a, c, t dbSNP:201290700
839 839 a, g dbSNP:774382622
840 840 g, t dbSNP:372946885
850 850 a, g dbSNP:759367396
851 851 a, g dbSNP:74375347
855 855 c, t dbSNP:776567252
860 860 a, c dbSNP:762027127
863 863 c, t dbSNP:765384989
868 868 g, t dbSNP:750810564
869 869 c, t dbSNP:142423874
872 872 c, g, t dbSNP:79095986
873 873 a, g dbSNP:755430626
875 875 a, g, t dbSNP:780823917
878 878 -, gtgggt dbSNP:745383395
878 878 c, t dbSNP:190517526
879 879 a, g dbSNP:367797052
880 880 a, t dbSNP:748913627
882 882 g, t dbSNP:371497237
888 888 a, g dbSNP:778556850
893 893 a, c, t dbSNP:199858417
894 894 a, g dbSNP:1139971
896 896 c, g, t dbSNP:150062713
900 900 c, t dbSNP:754907833
908 908 g, t dbSNP:777837925
915 915 c, t dbSNP:749272629
917 917 c, g dbSNP:74885383
921 921 a, g dbSNP:778967682
932 932 c, t dbSNP:746076379
933 933 c, t dbSNP:201405906
934 934 a, g dbSNP:545998821
938 938 c, t dbSNP:761266322
939 939 a, c, g dbSNP:142351354
941 941 c, g dbSNP:7107335
947 947 a, c, g, t dbSNP:201650491
948 948 a, g dbSNP:766230830
956 956 c, t dbSNP:751291553
957 957 a, g dbSNP:754925345
963 963 c, g dbSNP:781061734
966 966 c, t dbSNP:147912305
967 967 c, t dbSNP:201671713
968 968 c, g dbSNP:148874083
973 973 a, g dbSNP:773237459
977 977 a, g dbSNP:772351881
978 978 a, g dbSNP:780577629
983 983 c, g dbSNP:747327385
989 989 c, t dbSNP:376795165
996 996 c, t dbSNP:777044032
998 998 c, t dbSNP:370925717
1002 1002 a, g, t dbSNP:376175476
1003 1003 c, t dbSNP:762518913
1007 1007 a, g dbSNP:765960487
1008 1008 c, g, t dbSNP:751381541
1011 1011 c, g dbSNP:767273646
1014 1014 a, g dbSNP:752630534
1021 1021 a, g dbSNP:756070640
1028 1028 a, c dbSNP:778859812
1042 1042 c, t dbSNP:547161316
1065 1065 c, g dbSNP:529985982
1093 1093 cg, tc dbSNP:386753094
1093 1093 c, t dbSNP:4755266
1094 1094 c, g dbSNP:77781241
1109 1109 a, g dbSNP:569628883
1110 1110 a, g dbSNP:371456705
1194 1194 a, g dbSNP:536686665
1195 1195 c, t dbSNP:374896443
1209 1209 c, t dbSNP:71490073
1211 1211 c, t dbSNP:555408480
1224 1224 a, c dbSNP:116250454
1240 1240 a, g dbSNP:536180457
1244 1244 c, t dbSNP:546842038
1251 1251 a, g dbSNP:554375084
1261 1261 c, t dbSNP:193127050
1301 1301 a, g dbSNP:778679874
1311 1311 a, c dbSNP:539902023
1315 1315 a, g dbSNP:745846614
1326 1326 a, g dbSNP:1063031
1333 1333 c, g dbSNP:12225877
1336 1336 a, g dbSNP:576375816
1339 1339 c, t dbSNP:543364816
1340 1340 a, g dbSNP:572260612
1342 1342 a, g dbSNP:538794913
1349 1349 c, g dbSNP:759814577
1351 1351 c, t dbSNP:540960667
1352 1352 c, g dbSNP:371743094
1359 1359 a, g dbSNP:533024020
1380 1380 a, g dbSNP:113805595
1385 1385 a, g dbSNP:559005209
1391 1391 a, g dbSNP:112667982
1396 1396 a, g dbSNP:563192606
1409 1409 c, t dbSNP:530636550
1410 1410 a, g dbSNP:538247800
1413 1413 a, c dbSNP:6485552
1418 1418 a, c dbSNP:370894393
1429 1429 c, t dbSNP:548974243
1440 1440 c, t dbSNP:374579809
1441 1441 a, g dbSNP:145762348
1444 1444 c, t dbSNP:536141761
1455 1455 c, t dbSNP:780025065
1464 1464 a, c dbSNP:367590200
1473 1473 a, g dbSNP:111384781
1477 1477 a, g dbSNP:143013555
1479 1479 a, g dbSNP:539686462
1484 1484 c, t dbSNP:558340117
1485 1485 a, g dbSNP:576431366
1488 1488 c, t dbSNP:537163605
1489 1489 a, g dbSNP:555325573
1508 1508 c, t dbSNP:573724569
1539 1539 a, c dbSNP:540921974
1540 1540 c, t dbSNP:559427355
1541 1541 a, g dbSNP:577875518
1546 1546 c, t dbSNP:190156561
1547 1547 a, g dbSNP:563522625
1558 1558 c, t dbSNP:761198379
1574 1574 c, t dbSNP:530589270
1576 1576 -, c dbSNP:374072292
1585 1585 -, gcctgg dbSNP:528703148
1593 1593 c, g dbSNP:549135777
1594 1594 g, t dbSNP:560848356
1608 1608 c, t dbSNP:528140057
1615 1615 -, aaa dbSNP:752801473
1615 1615 -, a dbSNP:561662297
1616 1616 -, aa dbSNP:775954230
1633 1633 -, aa dbSNP:398045166
1639 1639 -, aa dbSNP:397848957

Target ORF information:

RefSeq Version NM_001024844
Organism Homo sapiens (human)
Definition Homo sapiens CD82 molecule (CD82), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001024844

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGGCTCAG CCTGTATCAA AGTCACCAAA TACTTTCTCT TCCTCTTCAA CTTGATCTTC 
TTTATCCTGG GCGCAGTGAT CCTGGGCTTC GGGGTGTGGA TCCTGGCCGA CAAGAGCAGT
TTCATCTCTG TCCTGCAAAC CTCCTCCAGC TCGCTTAGGA TGGGGGCCTA TGTCTTCATC
GGCGTGGGGG CAGTCACTAT GCTCATGGGC TTCCTGGGCT GCATCGGCGC CGTCAACGAG
GTCCGCTGCC TGCTGGGGCT GCTGAAGCAG GAGATGGGCG GCATCGTGAC TGAGCTCATT
CGAGACTACA ACAGCAGTCG CGAGGACAGC CTGCAGGATG CCTGGGACTA CGTGCAGGCT
CAGGTGAAGT GCTGCGGCTG GGTCAGCTTC TACAACTGGA CAGACAACGC TGAGCTCATG
AATCGCCCTG AGGTCACCTA CCCCTGTTCC TGCGAAGTCA AGGGGGAAGA GGACAACAGC
CTTTCTGTGA GGAAGGGCTT CTGCGAGGCC CCCGGCAACA GGACCCAGAG TGGCAACCAC
CCTGAGGACT GGCCTGTGTA CCAGGAGGGC TGCATGGAGA AGGTGCAGGC GTGGCTGCAG
GAGAACCTGG GCATCATCCT CGGCGTGGGC GTGGGTGTGG CCATCATCGA GCTCCTGGGG
ATGGTCCTGT CCATCTGCTT GTGCCGGCAC GTCCATTCCG AAGACTACAG CAAGGTCCCC
AAGTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.