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CD82 cDNA ORF clone, Homo sapiens (human)

Gene Symbol CD82
Entrez Gene ID 3732
Full Name CD82 molecule
Synonyms 4F9, C33, GR15, IA4, KAI1, R2, SAR2, ST6, TSPAN27
General protein information
Preferred Names
CD82 antigen
Names
CD82 antigen
tspan-27
C33 antigen
tetraspanin-27
inducible membrane protein R2
metastasis suppressor Kangai-1
kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4))
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11p11.2

Summary This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

600623

Disorder Html: {Prostate cancer, susceptibility to}, 176807 (2)

mRNA and Protein(s)

mRNA Protein Name
XM_006718222 XP_006718285 CD82 antigen isoform X2
XM_006718223 XP_006718286 CD82 antigen isoform X1
XM_011520067 XP_011518369 CD82 antigen isoform X3
NM_002231 NP_002222 CD82 antigen isoform 1
NM_001024844 NP_001020015 CD82 antigen isoform 2



Homo sapiens (human) CD82 NP_002222.1
Pan troglodytes (chimpanzee) CD82 XP_001159791.1
Macaca mulatta (Rhesus monkey) CD82 XP_002799617.1
Canis lupus familiaris (dog) CD82 XP_005631367.1
Bos taurus (cattle) LOC100335281 XP_005216571.1
Mus musculus (house mouse) Cd82 NP_001258360.1
Rattus norvegicus (Norway rat) Cd82 NP_113985.2
Gallus gallus (chicken) CD82 NP_001008470.1
Danio rerio (zebrafish) cd82a NP_997826.1
Danio rerio (zebrafish) cd82b NP_997828.1
Drosophila melanogaster (fruit fly) Tsp66E NP_523985.1
Caenorhabditis elegans tsp-8 NP_510445.1
Xenopus (Silurana) tropicalis (western clawed frog) cd82 NP_989191.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CD82 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CD82 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu44860 XM_006718222 PREDICTED: Homo sapiens CD82 molecule (CD82), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu26932 XM_006718223 PREDICTED: Homo sapiens CD82 molecule (CD82), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu58546 XM_011520067 PREDICTED: Homo sapiens CD82 molecule (CD82), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu26932 NM_002231 Homo sapiens CD82 molecule (CD82), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu26919 NM_001024844 Homo sapiens CD82 molecule (CD82), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $188.30
$269.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu44860
Clone ID Related Accession (Same CDS sequence) XM_006718222
Accession Version XM_006718222.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 927bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product CD82 antigen isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCCCTGG TCATTAACCA GAGTCCTCCC TGCTGCTGTG TGGACGACAC GTGGGCACAG 
GCAGAAGTGG GCCCTGTGAC CAGCTGCACT GGTTTCGTGG AAGGAAGCTC CAGGACTGGC
GGGATGGGCT CAGCCTGTAT CAAAGTCACC AAATACTTTC TCTTCCTCTT CAACTTGATC
TTCTTTATCC TGGGCGCAGT GATCCTGGGC TTCGGGGTGT GGATCCTGGC CGACAAGAGC
AGTTTCATCT CTGTCCTGCA AACCTCCTCC AGCTCGCTTA GGATGGGGGC CTATGTCTTC
ATCGGCGTGG GGGCAGTCAC TATGCTCATG GGCTTCCTGG GCTGCATCGG CGCCGTCAAC
GAGGTCCGCT GCCTGCTGGG GCTGTACTTT GCTTTCCTGC TCCTGATCCT CATTGCCCAG
GTGACGGCCG GGGCCCTCTT CTACTTCAAC ATGGGCAAGC TGAAGCAGGA GATGGGCGGC
ATCGTGACTG AGCTCATTCG AGACTACAAC AGCAGTCGCG AGGACAGCCT GCAGGATGCC
TGGGACTACG TGCAGGCTCA GGTGAAGTGC TGCGGCTGGG TCAGCTTCTA CAACTGGACA
GACAACGCTG AGCTCATGAA TCGCCCTGAG GTCACCTACC CCTGTTCCTG CGAAGTCAAG
GGGGAAGAGG ACAACAGCCT TTCTGTGAGG AAGGGCTTCT GCGAGGCCCC CGGCAACAGG
ACCCAGAGTG GCAACCACCC TGAGGACTGG CCTGTGTACC AGGAGGGCTG CATGGAGAAG
GTGCAGGCGT GGCTGCAGGA GAACCTGGGC ATCATCCTCG GCGTGGGCGT GGGTGTGGCC
ATCATCGAGC TCCTGGGGAT GGTCCTGTCC ATCTGCTTGT GCCGGCACGT CCATTCCGAA
GACTACAGCA AGGTCCCCAA GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006718285.1
CDS56..982
Misc Feature(1)248..946(+)
Misc Feature(2)494..865(+)
Misc Feature(3)500..619(+)
Translation

Target ORF information:

RefSeq Version XM_006718222
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens CD82 molecule (CD82), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006718222

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCCCTGG TCATTAACCA GAGTCCTCCC TGCTGCTGTG TGGACGACAC GTGGGCACAG 
GCAGAAGTGG GCCCTGTGAC CAGCTGCACT GGTTTCGTGG AAGGAAGCTC CAGGACTGGC
GGGATGGGCT CAGCCTGTAT CAAAGTCACC AAATACTTTC TCTTCCTCTT CAACTTGATC
TTCTTTATCC TGGGCGCAGT GATCCTGGGC TTCGGGGTGT GGATCCTGGC CGACAAGAGC
AGTTTCATCT CTGTCCTGCA AACCTCCTCC AGCTCGCTTA GGATGGGGGC CTATGTCTTC
ATCGGCGTGG GGGCAGTCAC TATGCTCATG GGCTTCCTGG GCTGCATCGG CGCCGTCAAC
GAGGTCCGCT GCCTGCTGGG GCTGTACTTT GCTTTCCTGC TCCTGATCCT CATTGCCCAG
GTGACGGCCG GGGCCCTCTT CTACTTCAAC ATGGGCAAGC TGAAGCAGGA GATGGGCGGC
ATCGTGACTG AGCTCATTCG AGACTACAAC AGCAGTCGCG AGGACAGCCT GCAGGATGCC
TGGGACTACG TGCAGGCTCA GGTGAAGTGC TGCGGCTGGG TCAGCTTCTA CAACTGGACA
GACAACGCTG AGCTCATGAA TCGCCCTGAG GTCACCTACC CCTGTTCCTG CGAAGTCAAG
GGGGAAGAGG ACAACAGCCT TTCTGTGAGG AAGGGCTTCT GCGAGGCCCC CGGCAACAGG
ACCCAGAGTG GCAACCACCC TGAGGACTGG CCTGTGTACC AGGAGGGCTG CATGGAGAAG
GTGCAGGCGT GGCTGCAGGA GAACCTGGGC ATCATCCTCG GCGTGGGCGT GGGTGTGGCC
ATCATCGAGC TCCTGGGGAT GGTCCTGTCC ATCTGCTTGT GCCGGCACGT CCATTCCGAA
GACTACAGCA AGGTCCCCAA GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26932
Clone ID Related Accession (Same CDS sequence) NM_002231 , XM_006718223
Accession Version XM_006718223.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 804bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product CD82 antigen isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGCTCAG CCTGTATCAA AGTCACCAAA TACTTTCTCT TCCTCTTCAA CTTGATCTTC 
TTTATCCTGG GCGCAGTGAT CCTGGGCTTC GGGGTGTGGA TCCTGGCCGA CAAGAGCAGT
TTCATCTCTG TCCTGCAAAC CTCCTCCAGC TCGCTTAGGA TGGGGGCCTA TGTCTTCATC
GGCGTGGGGG CAGTCACTAT GCTCATGGGC TTCCTGGGCT GCATCGGCGC CGTCAACGAG
GTCCGCTGCC TGCTGGGGCT GTACTTTGCT TTCCTGCTCC TGATCCTCAT TGCCCAGGTG
ACGGCCGGGG CCCTCTTCTA CTTCAACATG GGCAAGCTGA AGCAGGAGAT GGGCGGCATC
GTGACTGAGC TCATTCGAGA CTACAACAGC AGTCGCGAGG ACAGCCTGCA GGATGCCTGG
GACTACGTGC AGGCTCAGGT GAAGTGCTGC GGCTGGGTCA GCTTCTACAA CTGGACAGAC
AACGCTGAGC TCATGAATCG CCCTGAGGTC ACCTACCCCT GTTCCTGCGA AGTCAAGGGG
GAAGAGGACA ACAGCCTTTC TGTGAGGAAG GGCTTCTGCG AGGCCCCCGG CAACAGGACC
CAGAGTGGCA ACCACCCTGA GGACTGGCCT GTGTACCAGG AGGGCTGCAT GGAGAAGGTG
CAGGCGTGGC TGCAGGAGAA CCTGGGCATC ATCCTCGGCG TGGGCGTGGG TGTGGCCATC
ATCGAGCTCC TGGGGATGGT CCTGTCCATC TGCTTGTGCC GGCACGTCCA TTCCGAAGAC
TACAGCAAGG TCCCCAAGTA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006718286.1
CDS156..959
Misc Feature(1)225..923(+)
Misc Feature(2)471..842(+)
Misc Feature(3)477..596(+)
Translation

Target ORF information:

RefSeq Version XM_006718223
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens CD82 molecule (CD82), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006718223

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGCTCAG CCTGTATCAA AGTCACCAAA TACTTTCTCT TCCTCTTCAA CTTGATCTTC 
TTTATCCTGG GCGCAGTGAT CCTGGGCTTC GGGGTGTGGA TCCTGGCCGA CAAGAGCAGT
TTCATCTCTG TCCTGCAAAC CTCCTCCAGC TCGCTTAGGA TGGGGGCCTA TGTCTTCATC
GGCGTGGGGG CAGTCACTAT GCTCATGGGC TTCCTGGGCT GCATCGGCGC CGTCAACGAG
GTCCGCTGCC TGCTGGGGCT GTACTTTGCT TTCCTGCTCC TGATCCTCAT TGCCCAGGTG
ACGGCCGGGG CCCTCTTCTA CTTCAACATG GGCAAGCTGA AGCAGGAGAT GGGCGGCATC
GTGACTGAGC TCATTCGAGA CTACAACAGC AGTCGCGAGG ACAGCCTGCA GGATGCCTGG
GACTACGTGC AGGCTCAGGT GAAGTGCTGC GGCTGGGTCA GCTTCTACAA CTGGACAGAC
AACGCTGAGC TCATGAATCG CCCTGAGGTC ACCTACCCCT GTTCCTGCGA AGTCAAGGGG
GAAGAGGACA ACAGCCTTTC TGTGAGGAAG GGCTTCTGCG AGGCCCCCGG CAACAGGACC
CAGAGTGGCA ACCACCCTGA GGACTGGCCT GTGTACCAGG AGGGCTGCAT GGAGAAGGTG
CAGGCGTGGC TGCAGGAGAA CCTGGGCATC ATCCTCGGCG TGGGCGTGGG TGTGGCCATC
ATCGAGCTCC TGGGGATGGT CCTGTCCATC TGCTTGTGCC GGCACGTCCA TTCCGAAGAC
TACAGCAAGG TCCCCAAGTA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58546
Clone ID Related Accession (Same CDS sequence) XM_011520067
Accession Version XM_011520067.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 711bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product CD82 antigen isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGTCAGAGT TGGAGACGGC GTGTGGAAAG CCCATGAGGC ACAAAACCTC CTCCAGCTCG 
CTTAGGATGG GGGCCTATGT CTTCATCGGC GTGGGGGCAG TCACTATGCT CATGGGCTTC
CTGGGCTGCA TCGGCGCCGT CAACGAGGTC CGCTGCCTGC TGGGGCTGTA CTTTGCTTTC
CTGCTCCTGA TCCTCATTGC CCAGGTGACG GCCGGGGCCC TCTTCTACTT CAACATGGGC
AAGCTGAAGC AGGAGATGGG CGGCATCGTG ACTGAGCTCA TTCGAGACTA CAACAGCAGT
CGCGAGGACA GCCTGCAGGA TGCCTGGGAC TACGTGCAGG CTCAGGTGAA GTGCTGCGGC
TGGGTCAGCT TCTACAACTG GACAGACAAC GCTGAGCTCA TGAATCGCCC TGAGGTCACC
TACCCCTGTT CCTGCGAAGT CAAGGGGGAA GAGGACAACA GCCTTTCTGT GAGGAAGGGC
TTCTGCGAGG CCCCCGGCAA CAGGACCCAG AGTGGCAACC ACCCTGAGGA CTGGCCTGTG
TACCAGGAGG GCTGCATGGA GAAGGTGCAG GCGTGGCTGC AGGAGAACCT GGGCATCATC
CTCGGCGTGG GCGTGGGTGT GGCCATCATC GAGCTCCTGG GGATGGTCCT GTCCATCTGC
TTGTGCCGGC ACGTCCATTC CGAAGACTAC AGCAAGGTCC CCAAGTACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011518369.1
CDS25..735
Misc Feature(1)70..699(+)
Misc Feature(2)247..618(+)
Misc Feature(3)253..372(+)
Translation

Target ORF information:

RefSeq Version XM_011520067
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens CD82 molecule (CD82), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011520067

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGTCAGAGT TGGAGACGGC GTGTGGAAAG CCCATGAGGC ACAAAACCTC CTCCAGCTCG 
CTTAGGATGG GGGCCTATGT CTTCATCGGC GTGGGGGCAG TCACTATGCT CATGGGCTTC
CTGGGCTGCA TCGGCGCCGT CAACGAGGTC CGCTGCCTGC TGGGGCTGTA CTTTGCTTTC
CTGCTCCTGA TCCTCATTGC CCAGGTGACG GCCGGGGCCC TCTTCTACTT CAACATGGGC
AAGCTGAAGC AGGAGATGGG CGGCATCGTG ACTGAGCTCA TTCGAGACTA CAACAGCAGT
CGCGAGGACA GCCTGCAGGA TGCCTGGGAC TACGTGCAGG CTCAGGTGAA GTGCTGCGGC
TGGGTCAGCT TCTACAACTG GACAGACAAC GCTGAGCTCA TGAATCGCCC TGAGGTCACC
TACCCCTGTT CCTGCGAAGT CAAGGGGGAA GAGGACAACA GCCTTTCTGT GAGGAAGGGC
TTCTGCGAGG CCCCCGGCAA CAGGACCCAG AGTGGCAACC ACCCTGAGGA CTGGCCTGTG
TACCAGGAGG GCTGCATGGA GAAGGTGCAG GCGTGGCTGC AGGAGAACCT GGGCATCATC
CTCGGCGTGG GCGTGGGTGT GGCCATCATC GAGCTCCTGG GGATGGTCCT GTCCATCTGC
TTGTGCCGGC ACGTCCATTC CGAAGACTAC AGCAAGGTCC CCAAGTACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26932
Clone ID Related Accession (Same CDS sequence) NM_002231 , XM_006718223
Accession Version NM_002231.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 804bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product CD82 antigen isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB127804.1, U20770.1 and AC010768.10. This sequence is a reference standard in the RefSeqGene project. On Jun 15, 2005 this sequence version replaced gi:13259537. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225708.1, AK225410.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGCTCAG CCTGTATCAA AGTCACCAAA TACTTTCTCT TCCTCTTCAA CTTGATCTTC 
TTTATCCTGG GCGCAGTGAT CCTGGGCTTC GGGGTGTGGA TCCTGGCCGA CAAGAGCAGT
TTCATCTCTG TCCTGCAAAC CTCCTCCAGC TCGCTTAGGA TGGGGGCCTA TGTCTTCATC
GGCGTGGGGG CAGTCACTAT GCTCATGGGC TTCCTGGGCT GCATCGGCGC CGTCAACGAG
GTCCGCTGCC TGCTGGGGCT GTACTTTGCT TTCCTGCTCC TGATCCTCAT TGCCCAGGTG
ACGGCCGGGG CCCTCTTCTA CTTCAACATG GGCAAGCTGA AGCAGGAGAT GGGCGGCATC
GTGACTGAGC TCATTCGAGA CTACAACAGC AGTCGCGAGG ACAGCCTGCA GGATGCCTGG
GACTACGTGC AGGCTCAGGT GAAGTGCTGC GGCTGGGTCA GCTTCTACAA CTGGACAGAC
AACGCTGAGC TCATGAATCG CCCTGAGGTC ACCTACCCCT GTTCCTGCGA AGTCAAGGGG
GAAGAGGACA ACAGCCTTTC TGTGAGGAAG GGCTTCTGCG AGGCCCCCGG CAACAGGACC
CAGAGTGGCA ACCACCCTGA GGACTGGCCT GTGTACCAGG AGGGCTGCAT GGAGAAGGTG
CAGGCGTGGC TGCAGGAGAA CCTGGGCATC ATCCTCGGCG TGGGCGTGGG TGTGGCCATC
ATCGAGCTCC TGGGGATGGT CCTGTCCATC TGCTTGTGCC GGCACGTCCA TTCCGAAGAC
TACAGCAAGG TCCCCAAGTA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002222.1
CDS249..1052
Misc Feature(1)54..56(+)
Misc Feature(2)282..344(+)
Misc Feature(3)318..1016(+)
Misc Feature(4)408..464(+)
Misc Feature(5)498..578(+)
Misc Feature(6)564..935(+)
Misc Feature(7)570..689(+)
Misc Feature(8)633..635(+)
Misc Feature(9)717..719(+)
Misc Feature(10)840..842(+)
Misc Feature(11)933..998(+)
Exon (1)1..146
Gene:CD82
Gene Synonym:
Exon (2)147..228
Gene:CD82
Gene Synonym:
Exon (3)229..311
Gene:CD82
Gene Synonym:
Exon (4)312..384
Gene:CD82
Gene Synonym:
Exon (5)385..509
Gene:CD82
Gene Synonym:
Exon (6)510..584
Gene:CD82
Gene Synonym:
Exon (7)585..686
Gene:CD82
Gene Synonym:
Exon (8)687..890
Gene:CD82
Gene Synonym:
Exon (9)891..974
Gene:CD82
Gene Synonym:
Exon (10)975..1715
Gene:CD82
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002231
Organism Homo sapiens (human)
Definition Homo sapiens CD82 molecule (CD82), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002231

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGCTCAG CCTGTATCAA AGTCACCAAA TACTTTCTCT TCCTCTTCAA CTTGATCTTC 
TTTATCCTGG GCGCAGTGAT CCTGGGCTTC GGGGTGTGGA TCCTGGCCGA CAAGAGCAGT
TTCATCTCTG TCCTGCAAAC CTCCTCCAGC TCGCTTAGGA TGGGGGCCTA TGTCTTCATC
GGCGTGGGGG CAGTCACTAT GCTCATGGGC TTCCTGGGCT GCATCGGCGC CGTCAACGAG
GTCCGCTGCC TGCTGGGGCT GTACTTTGCT TTCCTGCTCC TGATCCTCAT TGCCCAGGTG
ACGGCCGGGG CCCTCTTCTA CTTCAACATG GGCAAGCTGA AGCAGGAGAT GGGCGGCATC
GTGACTGAGC TCATTCGAGA CTACAACAGC AGTCGCGAGG ACAGCCTGCA GGATGCCTGG
GACTACGTGC AGGCTCAGGT GAAGTGCTGC GGCTGGGTCA GCTTCTACAA CTGGACAGAC
AACGCTGAGC TCATGAATCG CCCTGAGGTC ACCTACCCCT GTTCCTGCGA AGTCAAGGGG
GAAGAGGACA ACAGCCTTTC TGTGAGGAAG GGCTTCTGCG AGGCCCCCGG CAACAGGACC
CAGAGTGGCA ACCACCCTGA GGACTGGCCT GTGTACCAGG AGGGCTGCAT GGAGAAGGTG
CAGGCGTGGC TGCAGGAGAA CCTGGGCATC ATCCTCGGCG TGGGCGTGGG TGTGGCCATC
ATCGAGCTCC TGGGGATGGT CCTGTCCATC TGCTTGTGCC GGCACGTCCA TTCCGAAGAC
TACAGCAAGG TCCCCAAGTA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26919
Clone ID Related Accession (Same CDS sequence) NM_001024844
Accession Version NM_001024844.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 729bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product CD82 antigen isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB127804.1, AY303776.1, U20770.1 and AC010768.10. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, as compared to variant 1. The encoded isoform (2) thus lacks a segment in the middle region, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY303776.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGGCTCAG CCTGTATCAA AGTCACCAAA TACTTTCTCT TCCTCTTCAA CTTGATCTTC 
TTTATCCTGG GCGCAGTGAT CCTGGGCTTC GGGGTGTGGA TCCTGGCCGA CAAGAGCAGT
TTCATCTCTG TCCTGCAAAC CTCCTCCAGC TCGCTTAGGA TGGGGGCCTA TGTCTTCATC
GGCGTGGGGG CAGTCACTAT GCTCATGGGC TTCCTGGGCT GCATCGGCGC CGTCAACGAG
GTCCGCTGCC TGCTGGGGCT GCTGAAGCAG GAGATGGGCG GCATCGTGAC TGAGCTCATT
CGAGACTACA ACAGCAGTCG CGAGGACAGC CTGCAGGATG CCTGGGACTA CGTGCAGGCT
CAGGTGAAGT GCTGCGGCTG GGTCAGCTTC TACAACTGGA CAGACAACGC TGAGCTCATG
AATCGCCCTG AGGTCACCTA CCCCTGTTCC TGCGAAGTCA AGGGGGAAGA GGACAACAGC
CTTTCTGTGA GGAAGGGCTT CTGCGAGGCC CCCGGCAACA GGACCCAGAG TGGCAACCAC
CCTGAGGACT GGCCTGTGTA CCAGGAGGGC TGCATGGAGA AGGTGCAGGC GTGGCTGCAG
GAGAACCTGG GCATCATCCT CGGCGTGGGC GTGGGTGTGG CCATCATCGA GCTCCTGGGG
ATGGTCCTGT CCATCTGCTT GTGCCGGCAC GTCCATTCCG AAGACTACAG CAAGGTCCCC
AAGTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001020015.1
CDS249..977
Misc Feature(1)54..56(+)
Misc Feature(2)318..941(+)
Misc Feature(3)504..860(+)
Misc Feature(4)510..614(+)
Misc Feature(5)558..560(+)
Misc Feature(6)642..644(+)
Misc Feature(7)765..767(+)
Exon (1)1..146
Gene:CD82
Gene Synonym:
Exon (2)147..228
Gene:CD82
Gene Synonym:
Exon (3)229..311
Gene:CD82
Gene Synonym:
Exon (4)312..384
Gene:CD82
Gene Synonym:
Exon (5)385..509
Gene:CD82
Gene Synonym:
Exon (6)510..611
Gene:CD82
Gene Synonym:
Exon (7)612..815
Gene:CD82
Gene Synonym:
Exon (8)816..899
Gene:CD82
Gene Synonym:
Exon (9)900..1640
Gene:CD82
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001024844
Organism Homo sapiens (human)
Definition Homo sapiens CD82 molecule (CD82), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001024844

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGGCTCAG CCTGTATCAA AGTCACCAAA TACTTTCTCT TCCTCTTCAA CTTGATCTTC 
TTTATCCTGG GCGCAGTGAT CCTGGGCTTC GGGGTGTGGA TCCTGGCCGA CAAGAGCAGT
TTCATCTCTG TCCTGCAAAC CTCCTCCAGC TCGCTTAGGA TGGGGGCCTA TGTCTTCATC
GGCGTGGGGG CAGTCACTAT GCTCATGGGC TTCCTGGGCT GCATCGGCGC CGTCAACGAG
GTCCGCTGCC TGCTGGGGCT GCTGAAGCAG GAGATGGGCG GCATCGTGAC TGAGCTCATT
CGAGACTACA ACAGCAGTCG CGAGGACAGC CTGCAGGATG CCTGGGACTA CGTGCAGGCT
CAGGTGAAGT GCTGCGGCTG GGTCAGCTTC TACAACTGGA CAGACAACGC TGAGCTCATG
AATCGCCCTG AGGTCACCTA CCCCTGTTCC TGCGAAGTCA AGGGGGAAGA GGACAACAGC
CTTTCTGTGA GGAAGGGCTT CTGCGAGGCC CCCGGCAACA GGACCCAGAG TGGCAACCAC
CCTGAGGACT GGCCTGTGTA CCAGGAGGGC TGCATGGAGA AGGTGCAGGC GTGGCTGCAG
GAGAACCTGG GCATCATCCT CGGCGTGGGC GTGGGTGTGG CCATCATCGA GCTCCTGGGG
ATGGTCCTGT CCATCTGCTT GTGCCGGCAC GTCCATTCCG AAGACTACAG CAAGGTCCCC
AAGTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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