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KCNA1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol KCNA1
Entrez Gene ID 3736
Full Name potassium channel, voltage gated shaker related subfamily A, member 1
Synonyms AEMK, EA1, HBK1, HUK1, KV1.1, MBK1, MK1, RBK1
General protein information
Preferred Names
potassium voltage-gated channel subfamily A member 1
Names
potassium voltage-gated channel subfamily A member 1
voltage-gated K(+) channel HuKI
voltage-gated potassium channel HBK1
voltage-gated potassium channel subunit Kv1.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12p13.32

Summary This gene encodes a voltage-gated delayed potassium channel that is phylogenetically related to the Drosophila Shaker channel. The encoded protein has six putative transmembrane segments (S1-S6), and the loop between S5 and S6 forms the pore and contains the conserved selectivity filter motif (GYGD). The functional channel is a homotetramer. The N-terminus of the channel is associated with beta subunits that can modify the inactivation properties of the channel as well as affect expression levels. The C-terminus of the channel is complexed to a PDZ domain protein that is responsible for channel targeting. Mutations in this gene have been associated with myokymia with periodic ataxia (AEMK). [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

176260

Disorder Html: Episodic ataxia/myokymia syndrome, 160120 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000217 NP_000208 potassium voltage-gated channel subfamily A member 1



Homo sapiens (human) KCNA1 NP_000208.2
Pan troglodytes (chimpanzee) KCNA1 XP_003313484.1
Macaca mulatta (Rhesus monkey) KCNA1 XP_001102027.2
Canis lupus familiaris (dog) KCNA1 XP_005637320.1
Bos taurus (cattle) KCNA1 XP_005207261.1
Mus musculus (house mouse) Kcna1 NP_034725.3
Rattus norvegicus (Norway rat) Kcna1 NP_775118.1
Gallus gallus (chicken) KCNA1 XP_004938130.1
Danio rerio (zebrafish) LOC795942 XP_005163101.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC100492822 XP_004912859.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following KCNA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KCNA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu25897
NM_000217 Homo sapiens potassium channel, voltage gated shaker related subfamily A, member 1 (KCNA1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
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30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25897
Clone ID Related Accession (Same CDS sequence) NM_000217
Accession Version NM_000217.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1488bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product potassium voltage-gated channel subfamily A member 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA236475.1 and AC006063.1. This sequence is a reference standard in the RefSeqGene project. On or before Dec 16, 2006 this sequence version replaced gi:113423206, gi:4557684. Sequence Note: This RefSeq record was created largely from genomic sequence because transcripts were not available for the entire length of the gene. This transcript is supported by sequences from mouse. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DA236475.1, DA244238.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACGGTGA TGTCTGGGGA GAACGTGGAC GAGGCTTCGG CCGCCCCGGG CCACCCCCAG 
GATGGCAGCT ACCCCCGGCA GGCCGACCAC GACGACCACG AGTGCTGCGA GCGCGTGGTG
ATCAACATCT CCGGGCTGCG CTTCGAGACG CAGCTCAAGA CCCTGGCGCA GTTCCCCAAC
ACGCTGCTGG GCAACCCTAA GAAACGCATG CGCTACTTCG ACCCCCTGAG GAACGAGTAC
TTCTTCGACC GCAACCGGCC CAGCTTCGAC GCCATCCTCT ACTACTACCA GTCCGGCGGC
CGCCTGCGGA GGCCGGTCAA CGTGCCCCTG GACATGTTCT CCGAGGAGAT CAAGTTTTAC
GAGTTGGGCG AGGAGGCCAT GGAGAAGTTC CGGGAGGACG AGGGCTTCAT CAAGGAGGAG
GAGCGCCCTC TGCCCGAGAA GGAGTACCAG CGCCAGGTGT GGCTGCTCTT CGAGTACCCC
GAGAGCTCGG GGCCCGCCAG GGTCATCGCC ATCGTCTCCG TCATGGTCAT CCTCATCTCC
ATCGTCATCT TTTGCCTGGA GACGCTCCCC GAGCTGAAGG ATGACAAGGA CTTCACGGGC
ACCGTCCACC GCATCGACAA CACCACGGTC ATCTACAATT CCAACATCTT CACAGACCCC
TTCTTCATCG TGGAAACGCT GTGTATCATC TGGTTCTCCT TCGAGCTGGT GGTGCGCTTC
TTCGCCTGCC CCAGCAAGAC GGACTTCTTC AAAAACATCA TGAACTTCAT AGACATTGTG
GCCATCATTC CTTATTTCAT CACGCTGGGC ACCGAGATAG CTGAGCAGGA AGGAAACCAG
AAGGGCGAGC AGGCCACCTC CCTGGCCATC CTCAGGGTCA TCCGCTTGGT AAGGGTTTTT
AGAATCTTCA AGCTCTCCCG CCACTCTAAG GGCCTCCAGA TCCTGGGCCA GACCCTCAAA
GCTAGTATGA GAGAGCTAGG GCTGCTCATC TTTTTCCTCT TCATCGGGGT CATCCTGTTT
TCTAGTGCAG TGTACTTTGC CGAGGCGGAA GAAGCTGAGT CGCACTTCTC CAGTATCCCC
GATGCTTTCT GGTGGGCGGT GGTGTCCATG ACCACTGTAG GATACGGTGA CATGTACCCT
GTGACAATTG GAGGCAAGAT CGTGGGCTCC TTGTGTGCCA TCGCTGGTGT GCTAACAATT
GCCCTGCCCG TACCTGTCAT TGTGTCCAAT TTCAACTATT TCTACCACCG AGAAACTGAG
GGGGAAGAGC AGGCTCAGTT GCTCCACGTC AGTTCCCCTA ACTTAGCCTC TGACAGTGAC
CTCAGTCGCC GCAGTTCCTC TACTATGAGC AAGTCTGAGT ACATGGAGAT CGAAGAGGAT
ATGAATAATA GCATAGCCCA TTATAGACAG GTCAATATCA GAACTGCCAA TTGCACCACT
GCTAACCAAA ACTGCGTTAA TAAGAGCAAG CTACTGACCG ATGTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000208.2
CDS1106..2593
Misc Feature(1)989..991(+)
Misc Feature(2)1220..1495(+)
Misc Feature(3)1607..1663(+)
Misc Feature(4)1766..1831(+)
Misc Feature(5)1772..2326(+)
Misc Feature(6)1865..1927(+)
Misc Feature(7)1973..2032(+)
Misc Feature(8)2069..2071(+)
Misc Feature(9)2081..2140(+)
Misc Feature(10)2099..2329(+)
Misc Feature(11)2219..2236(+)
Misc Feature(12)2264..2329(+)
Misc Feature(13)2438..2440(+)
Misc Feature(14)2582..2590(+)
Exon (1)1..566
Gene:KCNA1
Gene Synonym:
Exon (2)567..7983
Gene:KCNA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000217
Organism Homo sapiens (human)
Definition Homo sapiens potassium channel, voltage gated shaker related subfamily A, member 1 (KCNA1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000217

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACGGTGA TGTCTGGGGA GAACGTGGAC GAGGCTTCGG CCGCCCCGGG CCACCCCCAG 
GATGGCAGCT ACCCCCGGCA GGCCGACCAC GACGACCACG AGTGCTGCGA GCGCGTGGTG
ATCAACATCT CCGGGCTGCG CTTCGAGACG CAGCTCAAGA CCCTGGCGCA GTTCCCCAAC
ACGCTGCTGG GCAACCCTAA GAAACGCATG CGCTACTTCG ACCCCCTGAG GAACGAGTAC
TTCTTCGACC GCAACCGGCC CAGCTTCGAC GCCATCCTCT ACTACTACCA GTCCGGCGGC
CGCCTGCGGA GGCCGGTCAA CGTGCCCCTG GACATGTTCT CCGAGGAGAT CAAGTTTTAC
GAGTTGGGCG AGGAGGCCAT GGAGAAGTTC CGGGAGGACG AGGGCTTCAT CAAGGAGGAG
GAGCGCCCTC TGCCCGAGAA GGAGTACCAG CGCCAGGTGT GGCTGCTCTT CGAGTACCCC
GAGAGCTCGG GGCCCGCCAG GGTCATCGCC ATCGTCTCCG TCATGGTCAT CCTCATCTCC
ATCGTCATCT TTTGCCTGGA GACGCTCCCC GAGCTGAAGG ATGACAAGGA CTTCACGGGC
ACCGTCCACC GCATCGACAA CACCACGGTC ATCTACAATT CCAACATCTT CACAGACCCC
TTCTTCATCG TGGAAACGCT GTGTATCATC TGGTTCTCCT TCGAGCTGGT GGTGCGCTTC
TTCGCCTGCC CCAGCAAGAC GGACTTCTTC AAAAACATCA TGAACTTCAT AGACATTGTG
GCCATCATTC CTTATTTCAT CACGCTGGGC ACCGAGATAG CTGAGCAGGA AGGAAACCAG
AAGGGCGAGC AGGCCACCTC CCTGGCCATC CTCAGGGTCA TCCGCTTGGT AAGGGTTTTT
AGAATCTTCA AGCTCTCCCG CCACTCTAAG GGCCTCCAGA TCCTGGGCCA GACCCTCAAA
GCTAGTATGA GAGAGCTAGG GCTGCTCATC TTTTTCCTCT TCATCGGGGT CATCCTGTTT
TCTAGTGCAG TGTACTTTGC CGAGGCGGAA GAAGCTGAGT CGCACTTCTC CAGTATCCCC
GATGCTTTCT GGTGGGCGGT GGTGTCCATG ACCACTGTAG GATACGGTGA CATGTACCCT
GTGACAATTG GAGGCAAGAT CGTGGGCTCC TTGTGTGCCA TCGCTGGTGT GCTAACAATT
GCCCTGCCCG TACCTGTCAT TGTGTCCAAT TTCAACTATT TCTACCACCG AGAAACTGAG
GGGGAAGAGC AGGCTCAGTT GCTCCACGTC AGTTCCCCTA ACTTAGCCTC TGACAGTGAC
CTCAGTCGCC GCAGTTCCTC TACTATGAGC AAGTCTGAGT ACATGGAGAT CGAAGAGGAT
ATGAATAATA GCATAGCCCA TTATAGACAG GTCAATATCA GAACTGCCAA TTGCACCACT
GCTAACCAAA ACTGCGTTAA TAAGAGCAAG CTACTGACCG ATGTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

RNA editing in the central cavity as a mechanism to regulate surface expression of the voltage-gated potassium channel Kv1.1
J. Biol. Chem. 289 (39), 26762-26771 (2014)
Streit AK, Matschke LA, Dolga AM, Rinne S and Decher N.


book

Moving gating charges through the gating pore in a Kv channel voltage sensor
Proc. Natl. Acad. Sci. U.S.A. 111 (19), E1950-E1959 (2014)
Lacroix JJ, Hyde HC, Campos FV and Bezanilla F.


book

High-resolution molecular genomic autopsy reveals complex sudden unexpected death in epilepsy risk profile
Epilepsia 55 (2), E6-E12 (2014)
Klassen TL, Bomben VC, Patel A, Drabek J, Chen TT, Gu W, Zhang F, Chapman K, Lupski JR, Noebels JL and Goldman AM.


book

Genetics of dizziness: cerebellar and vestibular disorders
Curr. Opin. Neurol. 27 (1), 98-104 (2014)
Requena T, Espinosa-Sanchez JM and Lopez-Escamez JA.


book

Clustering of Shaker-type K+ channels by interaction with a family of membrane-associated guanylate kinases
Nature 378 (6552), 85-88 (1995)
Kim E, Niethammer M, Rothschild A, Jan YN and Sheng M.


book

Episodic ataxia/myokymia syndrome is associated with point mutations in the human potassium channel gene, KCNA1
Nat. Genet. 8 (2), 136-140 (1994)
Browne DL, Gancher ST, Nutt JG, Brunt ER, Smith EA, Kramer P and Litt M.


book

Episodic Ataxia Type 1
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
D'Adamo,M.C., Hanna,M.G., Di Giovanni,G. and Pessia,M.


book

Hereditary Ataxia Overview
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Bird,T.D.


book

Molecular cloning, characterization, and genomic localization of a human potassium channel gene
Genomics 12 (4), 729-737 (1992)
Curran ME, Landes GM and Keating MT.


book

Cloning and characterization of a cDNA encoding a human brain potassium channel
Biochem. Soc. Trans. 18 (5), 891-892 (1990)
Freeman SN, Conley EC, Brennand JC, Russell NJ and Brammar WJ.


 
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