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ACAT1 acetyl-CoA acetyltransferase 1 [Homo sapiens (human)]

Gene Symbol ACAT1
Entrez Gene ID 38
Full Name acetyl-CoA acetyltransferase 1
Synonyms ACAT, MAT, T2, THIL
General protein information
Preferred Names
acetyl-CoA acetyltransferase, mitochondrial
Names
acetyl-CoA acetyltransferase, mitochondrial
acetoacetyl-CoA thiolase
acetoacetyl Coenzyme A thiolase
acetyl-Coenzyme A acetyltransferase 1
mitochondrial acetoacetyl-CoA thiolase
NP_000010.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11q22.3

Summary This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]. lac of sum
Disorder MIM:

607809

Disorder Html: Alpha-methylacetoacetic aciduria, 203750 (3)





The following ACAT1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACAT1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu45759 XM_006718835 PREDICTED: Homo sapiens acetyl-CoA acetyltransferase 1 (ACAT1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $319
OHu45759 XM_006718834 PREDICTED: Homo sapiens acetyl-CoA acetyltransferase 1 (ACAT1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $319
OHu25199 NM_000019 Homo sapiens acetyl-CoA acetyltransferase 1 (ACAT1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $319

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu45759
Accession Version XM_006718835.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1014bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product acetyl-CoA acetyltransferase, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_033899.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578822177. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGTAATG TTCTACAAGG AGGTGAAGGA CAAGCTCCTA CAAGGCAGGC AGTATTGGGT 
GCAGGCTTAC CTATTTCTAC TCCATGTACC ACCATAAACA AAGTTTGTGC TTCAGGAATG
AAAGCCATCA TGATGGCCTC TCAAAGTCTT ATGTGTGGAC ATCAGGATGT GATGGTGGCA
GGTGGGATGG AGAGCATGTC CAATGTTCCA TATGTAATGA ACAGAGGATC AACACCATAT
GGTGGGGTAA AGCTTGAAGA TTTGATTGTA AAAGACGGGC TAACTGATGT CTACAATAAA
ATTCATATGG GCAGCTGTGC TGAGAATACA GCAAAGAAGC TGAATATTGC ACGAAATGAA
CAGGACGCTT ATGCTATTAA TTCTTATACC AGAAGTAAAG CAGCATGGGA AGCTGGGAAA
TTTGGAAATG AAGTTATTCC TGTCACAGTT ACAGTAAAAG GTCAACCAGA TGTAGTGGTG
AAAGAAGATG AAGAATATAA ACGTGTTGAT TTTAGCAAAG TTCCAAAGCT GAAGACAGTT
TTCCAGAAAG AAAATGGCAC AGTAACAGCT GCCAATGCCA GTACACTGAA TGATGGAGCA
GCTGCTCTGG TTCTCATGAC GGCAGATGCA GCGAAGAGGC TCAATGTTAC ACCACTGGCA
AGAATAGTAG CATTTGCTGA CGCTGCTGTA GAACCTATTG ATTTTCCAAT TGCTCCTGTA
TATGCTGCAT CTATGGTTCT TAAAGATGTG GGATTGAAAA AAGAAGATAT TGCAATGTGG
GAAGTAAATG AAGCCTTTAG TCTGGTTGTA CTAGCAAACA TTAAAATGTT GGAGATTGAT
CCCCAAAAAG TGAATATCAA TGGAGGAGCT GTTTCTCTGG GACATCCAAT TGGGATGTCT
GGAGCCAGGA TTGTTGGTCA TTTGACTCAT GCCTTGAAGC AAGGAGAATA CGGTCTTGCC
AGTATTTGCA ATGGAGGAGG AGGTGCTTCT GCCATGCTAA TTCAGAAGCT GTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006718898.1
CDS330..1343
Misc Feature(1)330..1337(+)
Misc Feature(2)363..1310(+)
Misc Feature(3)435..1298(+)
Translation
Position Chain Variation Link
56 56 -, c dbSNP:747512725
97 97 a, g dbSNP:181157510
113 113 a, g dbSNP:528347121
140 140 -, at dbSNP:749873354
141 141 c, t dbSNP:775374421
143 143 c, t dbSNP:760794308
144 144 g, t dbSNP:764142914
146 146 a, g dbSNP:777242705
150 150 c, t dbSNP:199952982
151 151 a, g dbSNP:765645574
159 159 a, g dbSNP:750824793
167 167 a, c dbSNP:759056115
172 172 c, t dbSNP:142869776
175 175 g, t dbSNP:752362759
188 188 c, t dbSNP:773917391
197 197 c, t dbSNP:763375578
198 198 a, g dbSNP:115763401
199 199 c, t dbSNP:545826042
208 208 -, c dbSNP:779565865
211 211 a, c dbSNP:760268967
214 214 c, t dbSNP:151080188
216 216 c, g dbSNP:753349314
230 230 a, c dbSNP:757128075
233 233 a, c, g dbSNP:147018814
272 272 a, c dbSNP:758176568
277 277 a, c dbSNP:779758622
284 284 c, t dbSNP:747201837
288 288 a, g dbSNP:772848693
295 295 c, g dbSNP:781366330
297 297 a, g dbSNP:748228668
303 303 c, t dbSNP:781564696
305 305 -, a dbSNP:34607623
312 312 g, t dbSNP:748425041
324 324 a, g dbSNP:756240301
325 325 c, g, t dbSNP:777738417
337 337 a, g dbSNP:120074145
342 342 c, t dbSNP:56061117
344 344 a, t dbSNP:771349233
346 346 a, t dbSNP:774717533
350 350 a, t dbSNP:746216662
362 362 a, g dbSNP:772492360
374 374 a, g dbSNP:776235078
377 377 a, g dbSNP:761498016
381 381 a, g dbSNP:764752367
384 384 c, t dbSNP:772625970
394 394 a, g dbSNP:747755121
400 400 c, g dbSNP:769333813
402 402 a, g dbSNP:138104334
407 407 c, t dbSNP:372836412
413 413 a, g dbSNP:762367702
415 415 a, g dbSNP:770518127
421 421 c, t dbSNP:774412064
427 427 a, g dbSNP:748835705
433 433 -, tt dbSNP:774418317
434 434 c, g, t dbSNP:767382364
437 437 c, g, t dbSNP:536301499
443 443 a, g dbSNP:374439037
453 453 c, g dbSNP:757252358
460 460 c, t dbSNP:779097442
480 480 a, t dbSNP:750876321
481 481 c, t dbSNP:758930842
492 492 c, g dbSNP:120074148
503 503 -, gg dbSNP:727503795
506 506 a, g dbSNP:765456788
508 508 a, c dbSNP:149522535
512 512 g, t dbSNP:773479251
514 514 c, g dbSNP:762991875
520 520 a, c dbSNP:766496849
526 526 c, t dbSNP:751962247
530 530 a, c, g dbSNP:35188041
531 531 a, g dbSNP:148639841
532 532 a, g dbSNP:199524907
539 539 a, g dbSNP:141131039
540 540 c, t dbSNP:768121096
541 541 a, g dbSNP:773728950
548 548 g, t dbSNP:778705529
561 561 a, g dbSNP:577313004
566 566 a, t dbSNP:201456540
579 579 a, g dbSNP:757956611
581 581 c, g dbSNP:146188525
582 582 c, g dbSNP:746877071
587 587 a, c dbSNP:768642780
591 591 c, t dbSNP:370063325
602 602 a, g dbSNP:747961254
605 605 c, t dbSNP:373393997
606 606 a, g dbSNP:120074141
627 627 -, a dbSNP:761038005
637 637 g, t dbSNP:541517496
666 666 a, c dbSNP:200498630
672 672 a, c dbSNP:748135542
681 681 c, t dbSNP:532190594
682 682 a, g dbSNP:370720208
684 684 a, g dbSNP:749446272
686 686 c, t dbSNP:771160678
694 694 a, g dbSNP:774499159
695 695 c, t dbSNP:759659537
696 696 a, g dbSNP:772087249
697 697 c, t dbSNP:776040489
701 701 g, t dbSNP:761282960
702 702 a, g dbSNP:764509842
704 704 -, atta dbSNP:764568533
708 708 a, g dbSNP:751034911
717 717 a, g dbSNP:199573646
728 728 a, c dbSNP:766041460
746 746 a, g dbSNP:545578010
753 753 a, g dbSNP:751235618
758 758 -, t dbSNP:777186620
768 768 a, c dbSNP:754478568
773 773 c, g dbSNP:780592247
780 780 a, g dbSNP:752592672
782 782 a, g dbSNP:756051455
811 811 -, aag dbSNP:765961551
812 812 a, g dbSNP:755683200
819 819 -, gaa dbSNP:751053463
829 829 a, c dbSNP:370652435
831 831 c, t dbSNP:753816946
832 832 a, g dbSNP:542087102
833 833 g, t dbSNP:778592070
835 835 a, t dbSNP:746059964
843 843 a, c dbSNP:563370385
847 847 a, g dbSNP:780221377
849 849 g, t dbSNP:747071980
869 869 c, t dbSNP:768923639
871 871 c, t dbSNP:747309345
873 873 c, t dbSNP:120074144
875 875 g, t dbSNP:139120939
900 900 a, g dbSNP:200640435
901 901 c, t dbSNP:765012030
903 903 a, c dbSNP:750195919
911 911 c, t dbSNP:200210306
917 917 a, g dbSNP:143892373
923 923 c, t dbSNP:747272327
926 926 a, c dbSNP:201725995
940 940 c, t dbSNP:781454450
944 944 c, t dbSNP:748623753
945 945 a, g, t dbSNP:368802454
950 950 a, g dbSNP:372169445
952 952 c, t dbSNP:771328733
958 958 a, c dbSNP:775103464
961 961 c, t dbSNP:747860630
962 962 a, g dbSNP:75811190
964 964 -, a dbSNP:398123097
966 966 a, t dbSNP:201180840
973 973 a, g dbSNP:562043215
977 977 a, t dbSNP:200318899
984 984 a, c dbSNP:750440809
987 987 g, t dbSNP:762624520
993 993 a, g dbSNP:766323857
994 994 c, t dbSNP:120074146
997 997 c, t dbSNP:755257455
999 999 c, g dbSNP:781364671
1000 1000 c, t dbSNP:750968102
1006 1006 c, t dbSNP:371656619
1008 1008 a, g dbSNP:780486838
1010 1010 c, t dbSNP:747714452
1027 1027 c, t dbSNP:755806238
1035 1035 c, g dbSNP:777350099
1037 1037 a, c dbSNP:73559264
1038 1038 a, g, t dbSNP:150038447
1043 1043 a, t dbSNP:745826234
1044 1044 a, c dbSNP:375119115
1047 1047 g, t dbSNP:775363663
1048 1048 c, t dbSNP:760748128
1049 1049 a, g dbSNP:764361167
1056 1056 c, g dbSNP:120074147
1062 1062 a, g dbSNP:369440498
1063 1063 c, t dbSNP:559903915
1081 1081 a, g dbSNP:80154645
1087 1087 a, g dbSNP:149171104
1088 1088 a, g dbSNP:763001817
1091 1091 -, ag dbSNP:781496140
1092 1092 -, gaa dbSNP:387906282
1092 1092 a, g dbSNP:766904962
1127 1127 c, t dbSNP:139657823
1134 1134 a, g dbSNP:759924404
1140 1140 c, t dbSNP:547532857
1142 1142 -, a dbSNP:387906283
1157 1157 g, t dbSNP:559365204
1158 1158 c, t dbSNP:756978324
1160 1160 a, g dbSNP:778512952
1165 1165 c, t dbSNP:749931948
1172 1172 c, t dbSNP:757908194
1175 1175 a, g dbSNP:780007425
1183 1183 a, g dbSNP:373771053
1190 1190 g, t dbSNP:751682437
1195 1195 g, t dbSNP:120074143
1197 1197 a, g dbSNP:120074140
1201 1201 c, t dbSNP:768448235
1218 1218 a, g dbSNP:780994047
1219 1219 a, c, t dbSNP:748303093
1222 1222 c, g dbSNP:773491386
1226 1226 a, g dbSNP:377295639
1231 1231 c, g dbSNP:371070695
1240 1240 c, t dbSNP:771006357
1247 1247 c, t dbSNP:774301980
1248 1248 a, c dbSNP:746332363
1254 1254 a, g dbSNP:772696101
1257 1257 c, t dbSNP:201183545
1258 1258 a, g dbSNP:761086326
1259 1259 c, t dbSNP:748749897
1261 1261 c, t dbSNP:764599801
1268 1268 a, g dbSNP:200729208
1275 1275 c, g dbSNP:762543361
1276 1276 a, g dbSNP:147872303
1280 1280 c, t dbSNP:367713788
1281 1281 a, g dbSNP:553101581
1288 1288 c, t dbSNP:767412638
1289 1289 c, t dbSNP:189339614
1290 1290 a, g dbSNP:755855638
1302 1302 -, gaggagga dbSNP:763847352
1306 1306 a, g dbSNP:777462355
1320 1320 a, g dbSNP:749463321
1334 1334 c, g dbSNP:757435663
1338 1338 a, c dbSNP:201500937
1341 1341 -, agac dbSNP:753615294
1347 1347 -, c dbSNP:111390656
1348 1348 c, t dbSNP:745942477
1349 1349 a, c dbSNP:772315169
1371 1371 c, t dbSNP:370288623
1373 1373 g, t dbSNP:747537829
1382 1382 -, aga dbSNP:750365525
1387 1387 a, g dbSNP:772007911
1391 1391 c, g dbSNP:778671746
1394 1394 a, g dbSNP:776862137
1395 1395 -, aatc dbSNP:758479564
1407 1407 a, c dbSNP:747994310
1457 1457 g, t dbSNP:373499678
1463 1463 -, ttaac dbSNP:556749538
1480 1480 a, t dbSNP:192326657
1483 1483 -, atg dbSNP:745386184
1501 1501 a, g dbSNP:771809384
1511 1511 -, taaaaa dbSNP:201268334
1522 1522 -, ttc dbSNP:372218361
1522 1522 g, t dbSNP:114728153
1527 1527 c, t dbSNP:773032425
1533 1533 c, g dbSNP:528994308
1557 1557 c, g dbSNP:145690535
1586 1586 c, t dbSNP:148934438
1593 1593 c, t dbSNP:79179095
1594 1594 a, g dbSNP:542328058
1601 1601 a, g dbSNP:79764488
1634 1634 a, c dbSNP:771132416
1636 1636 a, t dbSNP:73559273
1687 1687 c, t dbSNP:776705661
1698 1698 c, g dbSNP:114039574
1700 1700 a, c dbSNP:373983292
1708 1708 c, t dbSNP:145398051
1740 1740 c, t dbSNP:568860814
1758 1758 c, t dbSNP:760462304
1763 1763 a, t dbSNP:569215583
1808 1808 -, aatc dbSNP:537463051
1850 1850 a, g dbSNP:12271846
1896 1896 a, t dbSNP:183999318
1898 1898 a, g dbSNP:111376037
1917 1917 c, g dbSNP:775813923
1942 1942 a, c dbSNP:2280332
1963 1963 c, t dbSNP:534079630
1980 1980 a, g dbSNP:116471564
2033 2033 c, t dbSNP:574706448
2046 2046 a, t dbSNP:188826374
2050 2050 c, t dbSNP:181581446
2051 2051 a, g dbSNP:575165263
2079 2079 c, t dbSNP:776479087
2086 2086 -, ag dbSNP:563995916
2087 2087 -, ga dbSNP:373601338
2090 2090 -, tatc dbSNP:773677466
2096 2096 c, t dbSNP:186239800
2097 2097 a, g dbSNP:147695530

Target ORF information:

RefSeq Version XM_006718835
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens acetyl-CoA acetyltransferase 1 (ACAT1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006718835

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGTAATG TTCTACAAGG AGGTGAAGGA CAAGCTCCTA CAAGGCAGGC AGTATTGGGT 
GCAGGCTTAC CTATTTCTAC TCCATGTACC ACCATAAACA AAGTTTGTGC TTCAGGAATG
AAAGCCATCA TGATGGCCTC TCAAAGTCTT ATGTGTGGAC ATCAGGATGT GATGGTGGCA
GGTGGGATGG AGAGCATGTC CAATGTTCCA TATGTAATGA ACAGAGGATC AACACCATAT
GGTGGGGTAA AGCTTGAAGA TTTGATTGTA AAAGACGGGC TAACTGATGT CTACAATAAA
ATTCATATGG GCAGCTGTGC TGAGAATACA GCAAAGAAGC TGAATATTGC ACGAAATGAA
CAGGACGCTT ATGCTATTAA TTCTTATACC AGAAGTAAAG CAGCATGGGA AGCTGGGAAA
TTTGGAAATG AAGTTATTCC TGTCACAGTT ACAGTAAAAG GTCAACCAGA TGTAGTGGTG
AAAGAAGATG AAGAATATAA ACGTGTTGAT TTTAGCAAAG TTCCAAAGCT GAAGACAGTT
TTCCAGAAAG AAAATGGCAC AGTAACAGCT GCCAATGCCA GTACACTGAA TGATGGAGCA
GCTGCTCTGG TTCTCATGAC GGCAGATGCA GCGAAGAGGC TCAATGTTAC ACCACTGGCA
AGAATAGTAG CATTTGCTGA CGCTGCTGTA GAACCTATTG ATTTTCCAAT TGCTCCTGTA
TATGCTGCAT CTATGGTTCT TAAAGATGTG GGATTGAAAA AAGAAGATAT TGCAATGTGG
GAAGTAAATG AAGCCTTTAG TCTGGTTGTA CTAGCAAACA TTAAAATGTT GGAGATTGAT
CCCCAAAAAG TGAATATCAA TGGAGGAGCT GTTTCTCTGG GACATCCAAT TGGGATGTCT
GGAGCCAGGA TTGTTGGTCA TTTGACTCAT GCCTTGAAGC AAGGAGAATA CGGTCTTGCC
AGTATTTGCA ATGGAGGAGG AGGTGCTTCT GCCATGCTAA TTCAGAAGCT GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu45759
Accession Version XM_006718834.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1014bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product acetyl-CoA acetyltransferase, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_033899.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578822175. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGTAATG TTCTACAAGG AGGTGAAGGA CAAGCTCCTA CAAGGCAGGC AGTATTGGGT 
GCAGGCTTAC CTATTTCTAC TCCATGTACC ACCATAAACA AAGTTTGTGC TTCAGGAATG
AAAGCCATCA TGATGGCCTC TCAAAGTCTT ATGTGTGGAC ATCAGGATGT GATGGTGGCA
GGTGGGATGG AGAGCATGTC CAATGTTCCA TATGTAATGA ACAGAGGATC AACACCATAT
GGTGGGGTAA AGCTTGAAGA TTTGATTGTA AAAGACGGGC TAACTGATGT CTACAATAAA
ATTCATATGG GCAGCTGTGC TGAGAATACA GCAAAGAAGC TGAATATTGC ACGAAATGAA
CAGGACGCTT ATGCTATTAA TTCTTATACC AGAAGTAAAG CAGCATGGGA AGCTGGGAAA
TTTGGAAATG AAGTTATTCC TGTCACAGTT ACAGTAAAAG GTCAACCAGA TGTAGTGGTG
AAAGAAGATG AAGAATATAA ACGTGTTGAT TTTAGCAAAG TTCCAAAGCT GAAGACAGTT
TTCCAGAAAG AAAATGGCAC AGTAACAGCT GCCAATGCCA GTACACTGAA TGATGGAGCA
GCTGCTCTGG TTCTCATGAC GGCAGATGCA GCGAAGAGGC TCAATGTTAC ACCACTGGCA
AGAATAGTAG CATTTGCTGA CGCTGCTGTA GAACCTATTG ATTTTCCAAT TGCTCCTGTA
TATGCTGCAT CTATGGTTCT TAAAGATGTG GGATTGAAAA AAGAAGATAT TGCAATGTGG
GAAGTAAATG AAGCCTTTAG TCTGGTTGTA CTAGCAAACA TTAAAATGTT GGAGATTGAT
CCCCAAAAAG TGAATATCAA TGGAGGAGCT GTTTCTCTGG GACATCCAAT TGGGATGTCT
GGAGCCAGGA TTGTTGGTCA TTTGACTCAT GCCTTGAAGC AAGGAGAATA CGGTCTTGCC
AGTATTTGCA ATGGAGGAGG AGGTGCTTCT GCCATGCTAA TTCAGAAGCT GTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006718897.1
CDS288..1301
Misc Feature(1)288..1295(+)
Misc Feature(2)321..1268(+)
Misc Feature(3)393..1256(+)
Translation
Position Chain Variation Link
20 20 c, t dbSNP:749014469
34 34 c, g dbSNP:752534931
37 37 c, t dbSNP:758607392
60 60 a, g dbSNP:12280709
98 98 -, at dbSNP:749873354
99 99 c, t dbSNP:775374421
101 101 c, t dbSNP:760794308
102 102 g, t dbSNP:764142914
104 104 a, g dbSNP:777242705
108 108 c, t dbSNP:199952982
109 109 a, g dbSNP:765645574
117 117 a, g dbSNP:750824793
125 125 a, c dbSNP:759056115
130 130 c, t dbSNP:142869776
133 133 g, t dbSNP:752362759
146 146 c, t dbSNP:773917391
155 155 c, t dbSNP:763375578
156 156 a, g dbSNP:115763401
157 157 c, t dbSNP:545826042
166 166 -, c dbSNP:779565865
169 169 a, c dbSNP:760268967
172 172 c, t dbSNP:151080188
174 174 c, g dbSNP:753349314
188 188 a, c dbSNP:757128075
191 191 a, c, g dbSNP:147018814
230 230 a, c dbSNP:758176568
235 235 a, c dbSNP:779758622
242 242 c, t dbSNP:747201837
246 246 a, g dbSNP:772848693
253 253 c, g dbSNP:781366330
255 255 a, g dbSNP:748228668
261 261 c, t dbSNP:781564696
263 263 -, a dbSNP:34607623
270 270 g, t dbSNP:748425041
282 282 a, g dbSNP:756240301
283 283 c, g, t dbSNP:777738417
295 295 a, g dbSNP:120074145
300 300 c, t dbSNP:56061117
302 302 a, t dbSNP:771349233
304 304 a, t dbSNP:774717533
308 308 a, t dbSNP:746216662
320 320 a, g dbSNP:772492360
332 332 a, g dbSNP:776235078
335 335 a, g dbSNP:761498016
339 339 a, g dbSNP:764752367
342 342 c, t dbSNP:772625970
352 352 a, g dbSNP:747755121
358 358 c, g dbSNP:769333813
360 360 a, g dbSNP:138104334
365 365 c, t dbSNP:372836412
371 371 a, g dbSNP:762367702
373 373 a, g dbSNP:770518127
379 379 c, t dbSNP:774412064
385 385 a, g dbSNP:748835705
391 391 -, tt dbSNP:774418317
392 392 c, g, t dbSNP:767382364
395 395 c, g, t dbSNP:536301499
401 401 a, g dbSNP:374439037
411 411 c, g dbSNP:757252358
418 418 c, t dbSNP:779097442
438 438 a, t dbSNP:750876321
439 439 c, t dbSNP:758930842
450 450 c, g dbSNP:120074148
461 461 -, gg dbSNP:727503795
464 464 a, g dbSNP:765456788
466 466 a, c dbSNP:149522535
470 470 g, t dbSNP:773479251
472 472 c, g dbSNP:762991875
478 478 a, c dbSNP:766496849
484 484 c, t dbSNP:751962247
488 488 a, c, g dbSNP:35188041
489 489 a, g dbSNP:148639841
490 490 a, g dbSNP:199524907
497 497 a, g dbSNP:141131039
498 498 c, t dbSNP:768121096
499 499 a, g dbSNP:773728950
506 506 g, t dbSNP:778705529
519 519 a, g dbSNP:577313004
524 524 a, t dbSNP:201456540
537 537 a, g dbSNP:757956611
539 539 c, g dbSNP:146188525
540 540 c, g dbSNP:746877071
545 545 a, c dbSNP:768642780
549 549 c, t dbSNP:370063325
560 560 a, g dbSNP:747961254
563 563 c, t dbSNP:373393997
564 564 a, g dbSNP:120074141
585 585 -, a dbSNP:761038005
595 595 g, t dbSNP:541517496
624 624 a, c dbSNP:200498630
630 630 a, c dbSNP:748135542
639 639 c, t dbSNP:532190594
640 640 a, g dbSNP:370720208
642 642 a, g dbSNP:749446272
644 644 c, t dbSNP:771160678
652 652 a, g dbSNP:774499159
653 653 c, t dbSNP:759659537
654 654 a, g dbSNP:772087249
655 655 c, t dbSNP:776040489
659 659 g, t dbSNP:761282960
660 660 a, g dbSNP:764509842
662 662 -, atta dbSNP:764568533
666 666 a, g dbSNP:751034911
675 675 a, g dbSNP:199573646
686 686 a, c dbSNP:766041460
704 704 a, g dbSNP:545578010
711 711 a, g dbSNP:751235618
716 716 -, t dbSNP:777186620
726 726 a, c dbSNP:754478568
731 731 c, g dbSNP:780592247
738 738 a, g dbSNP:752592672
740 740 a, g dbSNP:756051455
769 769 -, aag dbSNP:765961551
770 770 a, g dbSNP:755683200
777 777 -, gaa dbSNP:751053463
787 787 a, c dbSNP:370652435
789 789 c, t dbSNP:753816946
790 790 a, g dbSNP:542087102
791 791 g, t dbSNP:778592070
793 793 a, t dbSNP:746059964
801 801 a, c dbSNP:563370385
805 805 a, g dbSNP:780221377
807 807 g, t dbSNP:747071980
827 827 c, t dbSNP:768923639
829 829 c, t dbSNP:747309345
831 831 c, t dbSNP:120074144
833 833 g, t dbSNP:139120939
858 858 a, g dbSNP:200640435
859 859 c, t dbSNP:765012030
861 861 a, c dbSNP:750195919
869 869 c, t dbSNP:200210306
875 875 a, g dbSNP:143892373
881 881 c, t dbSNP:747272327
884 884 a, c dbSNP:201725995
898 898 c, t dbSNP:781454450
902 902 c, t dbSNP:748623753
903 903 a, g, t dbSNP:368802454
908 908 a, g dbSNP:372169445
910 910 c, t dbSNP:771328733
916 916 a, c dbSNP:775103464
919 919 c, t dbSNP:747860630
920 920 a, g dbSNP:75811190
922 922 -, a dbSNP:398123097
924 924 a, t dbSNP:201180840
931 931 a, g dbSNP:562043215
935 935 a, t dbSNP:200318899
942 942 a, c dbSNP:750440809
945 945 g, t dbSNP:762624520
951 951 a, g dbSNP:766323857
952 952 c, t dbSNP:120074146
955 955 c, t dbSNP:755257455
957 957 c, g dbSNP:781364671
958 958 c, t dbSNP:750968102
964 964 c, t dbSNP:371656619
966 966 a, g dbSNP:780486838
968 968 c, t dbSNP:747714452
985 985 c, t dbSNP:755806238
993 993 c, g dbSNP:777350099
995 995 a, c dbSNP:73559264
996 996 a, g, t dbSNP:150038447
1001 1001 a, t dbSNP:745826234
1002 1002 a, c dbSNP:375119115
1005 1005 g, t dbSNP:775363663
1006 1006 c, t dbSNP:760748128
1007 1007 a, g dbSNP:764361167
1014 1014 c, g dbSNP:120074147
1020 1020 a, g dbSNP:369440498
1021 1021 c, t dbSNP:559903915
1039 1039 a, g dbSNP:80154645
1045 1045 a, g dbSNP:149171104
1046 1046 a, g dbSNP:763001817
1049 1049 -, ag dbSNP:781496140
1050 1050 -, gaa dbSNP:387906282
1050 1050 a, g dbSNP:766904962
1085 1085 c, t dbSNP:139657823
1092 1092 a, g dbSNP:759924404
1098 1098 c, t dbSNP:547532857
1100 1100 -, a dbSNP:387906283
1115 1115 g, t dbSNP:559365204
1116 1116 c, t dbSNP:756978324
1118 1118 a, g dbSNP:778512952
1123 1123 c, t dbSNP:749931948
1130 1130 c, t dbSNP:757908194
1133 1133 a, g dbSNP:780007425
1141 1141 a, g dbSNP:373771053
1148 1148 g, t dbSNP:751682437
1153 1153 g, t dbSNP:120074143
1155 1155 a, g dbSNP:120074140
1159 1159 c, t dbSNP:768448235
1176 1176 a, g dbSNP:780994047
1177 1177 a, c, t dbSNP:748303093
1180 1180 c, g dbSNP:773491386
1184 1184 a, g dbSNP:377295639
1189 1189 c, g dbSNP:371070695
1198 1198 c, t dbSNP:771006357
1205 1205 c, t dbSNP:774301980
1206 1206 a, c dbSNP:746332363
1212 1212 a, g dbSNP:772696101
1215 1215 c, t dbSNP:201183545
1216 1216 a, g dbSNP:761086326
1217 1217 c, t dbSNP:748749897
1219 1219 c, t dbSNP:764599801
1226 1226 a, g dbSNP:200729208
1233 1233 c, g dbSNP:762543361
1234 1234 a, g dbSNP:147872303
1238 1238 c, t dbSNP:367713788
1239 1239 a, g dbSNP:553101581
1246 1246 c, t dbSNP:767412638
1247 1247 c, t dbSNP:189339614
1248 1248 a, g dbSNP:755855638
1260 1260 -, gaggagga dbSNP:763847352
1264 1264 a, g dbSNP:777462355
1278 1278 a, g dbSNP:749463321
1292 1292 c, g dbSNP:757435663
1296 1296 a, c dbSNP:201500937
1299 1299 -, agac dbSNP:753615294
1305 1305 -, c dbSNP:111390656
1306 1306 c, t dbSNP:745942477
1307 1307 a, c dbSNP:772315169
1329 1329 c, t dbSNP:370288623
1331 1331 g, t dbSNP:747537829
1340 1340 -, aga dbSNP:750365525
1345 1345 a, g dbSNP:772007911
1349 1349 c, g dbSNP:778671746
1352 1352 a, g dbSNP:776862137
1353 1353 -, aatc dbSNP:758479564
1365 1365 a, c dbSNP:747994310
1415 1415 g, t dbSNP:373499678
1421 1421 -, ttaac dbSNP:556749538
1438 1438 a, t dbSNP:192326657
1441 1441 -, atg dbSNP:745386184
1459 1459 a, g dbSNP:771809384
1469 1469 -, taaaaa dbSNP:201268334
1480 1480 -, ttc dbSNP:372218361
1480 1480 g, t dbSNP:114728153
1485 1485 c, t dbSNP:773032425
1491 1491 c, g dbSNP:528994308
1515 1515 c, g dbSNP:145690535
1544 1544 c, t dbSNP:148934438
1551 1551 c, t dbSNP:79179095
1552 1552 a, g dbSNP:542328058
1559 1559 a, g dbSNP:79764488
1592 1592 a, c dbSNP:771132416
1594 1594 a, t dbSNP:73559273
1645 1645 c, t dbSNP:776705661
1656 1656 c, g dbSNP:114039574
1658 1658 a, c dbSNP:373983292
1666 1666 c, t dbSNP:145398051
1698 1698 c, t dbSNP:568860814
1716 1716 c, t dbSNP:760462304
1721 1721 a, t dbSNP:569215583
1766 1766 -, aatc dbSNP:537463051
1808 1808 a, g dbSNP:12271846
1854 1854 a, t dbSNP:183999318
1856 1856 a, g dbSNP:111376037
1875 1875 c, g dbSNP:775813923
1900 1900 a, c dbSNP:2280332
1921 1921 c, t dbSNP:534079630
1938 1938 a, g dbSNP:116471564
1991 1991 c, t dbSNP:574706448
2004 2004 a, t dbSNP:188826374
2008 2008 c, t dbSNP:181581446
2009 2009 a, g dbSNP:575165263
2037 2037 c, t dbSNP:776479087
2044 2044 -, ag dbSNP:563995916
2045 2045 -, ga dbSNP:373601338
2048 2048 -, tatc dbSNP:773677466
2054 2054 c, t dbSNP:186239800
2055 2055 a, g dbSNP:147695530

Target ORF information:

RefSeq Version XM_006718834
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens acetyl-CoA acetyltransferase 1 (ACAT1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006718834

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGTAATG TTCTACAAGG AGGTGAAGGA CAAGCTCCTA CAAGGCAGGC AGTATTGGGT 
GCAGGCTTAC CTATTTCTAC TCCATGTACC ACCATAAACA AAGTTTGTGC TTCAGGAATG
AAAGCCATCA TGATGGCCTC TCAAAGTCTT ATGTGTGGAC ATCAGGATGT GATGGTGGCA
GGTGGGATGG AGAGCATGTC CAATGTTCCA TATGTAATGA ACAGAGGATC AACACCATAT
GGTGGGGTAA AGCTTGAAGA TTTGATTGTA AAAGACGGGC TAACTGATGT CTACAATAAA
ATTCATATGG GCAGCTGTGC TGAGAATACA GCAAAGAAGC TGAATATTGC ACGAAATGAA
CAGGACGCTT ATGCTATTAA TTCTTATACC AGAAGTAAAG CAGCATGGGA AGCTGGGAAA
TTTGGAAATG AAGTTATTCC TGTCACAGTT ACAGTAAAAG GTCAACCAGA TGTAGTGGTG
AAAGAAGATG AAGAATATAA ACGTGTTGAT TTTAGCAAAG TTCCAAAGCT GAAGACAGTT
TTCCAGAAAG AAAATGGCAC AGTAACAGCT GCCAATGCCA GTACACTGAA TGATGGAGCA
GCTGCTCTGG TTCTCATGAC GGCAGATGCA GCGAAGAGGC TCAATGTTAC ACCACTGGCA
AGAATAGTAG CATTTGCTGA CGCTGCTGTA GAACCTATTG ATTTTCCAAT TGCTCCTGTA
TATGCTGCAT CTATGGTTCT TAAAGATGTG GGATTGAAAA AAGAAGATAT TGCAATGTGG
GAAGTAAATG AAGCCTTTAG TCTGGTTGTA CTAGCAAACA TTAAAATGTT GGAGATTGAT
CCCCAAAAAG TGAATATCAA TGGAGGAGCT GTTTCTCTGG GACATCCAAT TGGGATGTCT
GGAGCCAGGA TTGTTGGTCA TTTGACTCAT GCCTTGAAGC AAGGAGAATA CGGTCTTGCC
AGTATTTGCA ATGGAGGAGG AGGTGCTTCT GCCATGCTAA TTCAGAAGCT GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25199
Accession Version NM_000019.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product acetyl-CoA acetyltransferase, mitochondrial precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D90228.1, AP002433.5, AW081574.1 and BM789408.1. This sequence is a reference standard in the RefSeqGene project. On Feb 20, 2009 this sequence version replaced gi:31563501. Summary: This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: D90228.1, AK312574.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCTGTGC TGGCGGCACT TCTGCGCAGC GGCGCCCGCA GCCGCAGCCC CCTGCTCCGG 
AGGCTGGTGC AGGAAATAAG ATATGTGGAA CGGAGTTATG TATCAAAACC CACTTTGAAG
GAAGTGGTCA TAGTAAGTGC TACAAGAACA CCCATTGGAT CTTTTTTAGG CAGCCTTTCC
TTGCTGCCAG CCACTAAGCT TGGTTCCATT GCAATTCAGG GAGCCATTGA AAAGGCAGGG
ATTCCAAAAG AAGAAGTGAA AGAAGCATAC ATGGGTAATG TTCTACAAGG AGGTGAAGGA
CAAGCTCCTA CAAGGCAGGC AGTATTGGGT GCAGGCTTAC CTATTTCTAC TCCATGTACC
ACCATAAACA AAGTTTGTGC TTCAGGAATG AAAGCCATCA TGATGGCCTC TCAAAGTCTT
ATGTGTGGAC ATCAGGATGT GATGGTGGCA GGTGGGATGG AGAGCATGTC CAATGTTCCA
TATGTAATGA ACAGAGGATC AACACCATAT GGTGGGGTAA AGCTTGAAGA TTTGATTGTA
AAAGACGGGC TAACTGATGT CTACAATAAA ATTCATATGG GCAGCTGTGC TGAGAATACA
GCAAAGAAGC TGAATATTGC ACGAAATGAA CAGGACGCTT ATGCTATTAA TTCTTATACC
AGAAGTAAAG CAGCATGGGA AGCTGGGAAA TTTGGAAATG AAGTTATTCC TGTCACAGTT
ACAGTAAAAG GTCAACCAGA TGTAGTGGTG AAAGAAGATG AAGAATATAA ACGTGTTGAT
TTTAGCAAAG TTCCAAAGCT GAAGACAGTT TTCCAGAAAG AAAATGGCAC AGTAACAGCT
GCCAATGCCA GTACACTGAA TGATGGAGCA GCTGCTCTGG TTCTCATGAC GGCAGATGCA
GCGAAGAGGC TCAATGTTAC ACCACTGGCA AGAATAGTAG CATTTGCTGA CGCTGCTGTA
GAACCTATTG ATTTTCCAAT TGCTCCTGTA TATGCTGCAT CTATGGTTCT TAAAGATGTG
GGATTGAAAA AAGAAGATAT TGCAATGTGG GAAGTAAATG AAGCCTTTAG TCTGGTTGTA
CTAGCAAACA TTAAAATGTT GGAGATTGAT CCCCAAAAAG TGAATATCAA TGGAGGAGCT
GTTTCTCTGG GACATCCAAT TGGGATGTCT GGAGCCAGGA TTGTTGGTCA TTTGACTCAT
GCCTTGAAGC AAGGAGAATA CGGTCTTGCC AGTATTTGCA ATGGAGGAGG AGGTGCTTCT
GCCATGCTAA TTCAGAAGCT GTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000010.1
CDS77..1360
Misc Feature(1)194..1357(+)
Misc Feature(2)203..1354(+)
Misc Feature(3)257..1327(+)
Misc Feature(4)452..1315(+)
Misc Feature(5)596..598(+)
Misc Feature(6)617..619(+)
Misc Feature(7)827..829(+)
Misc Feature(8)848..856(+)
Misc Feature(9)863..865(+)
Exon (1)1..148
Gene:ACAT1
Gene Synonym:
Exon (2)149..196
Gene:ACAT1
Gene Synonym:
Exon (3)197..314
Gene:ACAT1
Gene Synonym:
Exon (4)315..410
Gene:ACAT1
Gene Synonym:
Exon (5)411..511
Gene:ACAT1
Gene Synonym:
Exon (6)512..655
Gene:ACAT1
Gene Synonym:
Exon (7)656..806
Gene:ACAT1
Gene Synonym:
Exon (8)807..902
Gene:ACAT1
Gene Synonym:
Exon (9)903..1016
Gene:ACAT1
Gene Synonym:
Exon (10)1017..1081
Gene:ACAT1
Gene Synonym:
Exon (11)1082..1239
Gene:ACAT1
Gene Synonym:
Exon (12)1240..2138
Gene:ACAT1
Gene Synonym:
Translation
Position Chain Variation Link
7 7 a, t dbSNP:566741161
44 44 a, g dbSNP:751838024
45 45 a, c dbSNP:759608984
49 49 a, t dbSNP:36216231
52 52 a, g dbSNP:753124881
54 54 a, c dbSNP:756666173
55 55 c, g, t dbSNP:3741054
57 57 a, g dbSNP:758065179
58 58 c, t dbSNP:556629486
61 61 g, t dbSNP:779836916
62 62 c, g dbSNP:113348145
64 64 g, t dbSNP:754411997
65 65 a, c, t dbSNP:112219560
67 67 c, t dbSNP:373627341
68 68 -, c dbSNP:34512802
68 68 a, t dbSNP:3741055
72 72 a, c dbSNP:747790910
73 73 g, t dbSNP:772828488
76 76 c, t dbSNP:749073683
78 78 a, t dbSNP:120074142
83 83 a, g dbSNP:770780507
86 86 a, c dbSNP:774524997
89 89 c, g dbSNP:11540420
89 89 c, g dbSNP:3741056
90 90 c, t dbSNP:767448873
98 98 c, t dbSNP:775668624
109 109 c, g dbSNP:761018640
110 110 a, g dbSNP:764674778
114 114 a, g dbSNP:754453264
119 119 c, t dbSNP:757688736
125 125 c, t dbSNP:573208198
127 127 a, c dbSNP:765733417
133 133 c, t dbSNP:540416798
136 136 c, g, t dbSNP:77311724
143 143 c, g dbSNP:375362239
157 157 -, at dbSNP:749873354
158 158 c, t dbSNP:775374421
160 160 c, t dbSNP:760794308
161 161 g, t dbSNP:764142914
163 163 a, g dbSNP:777242705
167 167 c, t dbSNP:199952982
168 168 a, g dbSNP:765645574
176 176 a, g dbSNP:750824793
184 184 a, c dbSNP:759056115
189 189 c, t dbSNP:142869776
192 192 g, t dbSNP:752362759
205 205 c, t dbSNP:773917391
214 214 c, t dbSNP:763375578
215 215 a, g dbSNP:115763401
216 216 c, t dbSNP:545826042
225 225 -, c dbSNP:779565865
228 228 a, c dbSNP:760268967
231 231 c, t dbSNP:151080188
233 233 c, g dbSNP:753349314
247 247 a, c dbSNP:757128075
250 250 a, c, g dbSNP:147018814
289 289 a, c dbSNP:758176568
294 294 a, c dbSNP:779758622
301 301 c, t dbSNP:747201837
305 305 a, g dbSNP:772848693
312 312 c, g dbSNP:781366330
314 314 a, g dbSNP:748228668
320 320 c, t dbSNP:781564696
322 322 -, a dbSNP:34607623
329 329 g, t dbSNP:748425041
341 341 a, g dbSNP:756240301
342 342 c, g, t dbSNP:777738417
354 354 a, g dbSNP:120074145
359 359 c, t dbSNP:56061117
361 361 a, t dbSNP:771349233
363 363 a, t dbSNP:774717533
367 367 a, t dbSNP:746216662
379 379 a, g dbSNP:772492360
391 391 a, g dbSNP:776235078
394 394 a, g dbSNP:761498016
398 398 a, g dbSNP:764752367
401 401 c, t dbSNP:772625970
411 411 a, g dbSNP:747755121
417 417 c, g dbSNP:769333813
419 419 a, g dbSNP:138104334
424 424 c, t dbSNP:372836412
430 430 a, g dbSNP:762367702
432 432 a, g dbSNP:770518127
438 438 c, t dbSNP:774412064
444 444 a, g dbSNP:748835705
450 450 -, tt dbSNP:774418317
451 451 c, g, t dbSNP:767382364
454 454 c, g, t dbSNP:536301499
460 460 a, g dbSNP:374439037
470 470 c, g dbSNP:757252358
477 477 c, t dbSNP:779097442
497 497 a, t dbSNP:750876321
498 498 c, t dbSNP:758930842
509 509 c, g dbSNP:120074148
520 520 -, gg dbSNP:727503795
523 523 a, g dbSNP:765456788
525 525 a, c dbSNP:149522535
529 529 g, t dbSNP:773479251
531 531 c, g dbSNP:762991875
537 537 a, c dbSNP:766496849
543 543 c, t dbSNP:751962247
547 547 a, c, g dbSNP:35188041
548 548 a, g dbSNP:148639841
549 549 a, g dbSNP:199524907
556 556 a, g dbSNP:141131039
557 557 c, t dbSNP:768121096
558 558 a, g dbSNP:773728950
565 565 g, t dbSNP:778705529
578 578 a, g dbSNP:577313004
583 583 a, t dbSNP:201456540
596 596 a, g dbSNP:757956611
598 598 c, g dbSNP:146188525
599 599 c, g dbSNP:746877071
604 604 a, c dbSNP:768642780
608 608 c, t dbSNP:370063325
619 619 a, g dbSNP:747961254
622 622 c, t dbSNP:373393997
623 623 a, g dbSNP:120074141
644 644 -, a dbSNP:761038005
654 654 g, t dbSNP:541517496
683 683 a, c dbSNP:200498630
689 689 a, c dbSNP:748135542
698 698 c, t dbSNP:532190594
699 699 a, g dbSNP:370720208
701 701 a, g dbSNP:749446272
703 703 c, t dbSNP:771160678
711 711 a, g dbSNP:774499159
712 712 c, t dbSNP:759659537
713 713 a, g dbSNP:772087249
714 714 c, t dbSNP:776040489
718 718 g, t dbSNP:761282960
719 719 a, g dbSNP:764509842
721 721 -, atta dbSNP:764568533
725 725 a, g dbSNP:751034911
734 734 a, g dbSNP:199573646
745 745 a, c dbSNP:766041460
763 763 a, g dbSNP:545578010
770 770 a, g dbSNP:751235618
775 775 -, t dbSNP:777186620
785 785 a, c dbSNP:754478568
790 790 c, g dbSNP:780592247
797 797 a, g dbSNP:752592672
799 799 a, g dbSNP:756051455
828 828 -, aag dbSNP:765961551
829 829 a, g dbSNP:755683200
836 836 -, gaa dbSNP:751053463
846 846 a, c dbSNP:370652435
848 848 c, t dbSNP:753816946
849 849 a, g dbSNP:542087102
850 850 g, t dbSNP:778592070
852 852 a, t dbSNP:746059964
860 860 a, c dbSNP:563370385
864 864 a, g dbSNP:780221377
866 866 g, t dbSNP:747071980
886 886 c, t dbSNP:768923639
888 888 c, t dbSNP:747309345
890 890 c, t dbSNP:120074144
892 892 g, t dbSNP:139120939
917 917 a, g dbSNP:200640435
918 918 c, t dbSNP:765012030
920 920 a, c dbSNP:750195919
928 928 c, t dbSNP:200210306
934 934 a, g dbSNP:143892373
940 940 c, t dbSNP:747272327
943 943 a, c dbSNP:201725995
957 957 c, t dbSNP:781454450
961 961 c, t dbSNP:748623753
962 962 a, g, t dbSNP:368802454
967 967 a, g dbSNP:372169445
969 969 c, t dbSNP:771328733
975 975 a, c dbSNP:775103464
978 978 c, t dbSNP:747860630
979 979 a, g dbSNP:75811190
981 981 -, a dbSNP:398123097
983 983 a, t dbSNP:201180840
990 990 a, g dbSNP:562043215
994 994 a, t dbSNP:200318899
1001 1001 a, c dbSNP:750440809
1004 1004 g, t dbSNP:762624520
1010 1010 a, g dbSNP:766323857
1011 1011 c, t dbSNP:120074146
1014 1014 c, t dbSNP:755257455
1016 1016 c, g dbSNP:781364671
1017 1017 c, t dbSNP:750968102
1023 1023 c, t dbSNP:371656619
1025 1025 a, g dbSNP:780486838
1027 1027 c, t dbSNP:747714452
1044 1044 c, t dbSNP:755806238
1052 1052 c, g dbSNP:777350099
1054 1054 a, c dbSNP:73559264
1055 1055 a, g, t dbSNP:150038447
1060 1060 a, t dbSNP:745826234
1061 1061 a, c dbSNP:375119115
1064 1064 g, t dbSNP:775363663
1065 1065 c, t dbSNP:760748128
1066 1066 a, g dbSNP:764361167
1073 1073 c, g dbSNP:120074147
1079 1079 a, g dbSNP:369440498
1080 1080 c, t dbSNP:559903915
1098 1098 a, g dbSNP:80154645
1104 1104 a, g dbSNP:149171104
1105 1105 a, g dbSNP:763001817
1108 1108 -, ag dbSNP:781496140
1109 1109 -, gaa dbSNP:387906282
1109 1109 a, g dbSNP:766904962
1144 1144 c, t dbSNP:139657823
1151 1151 a, g dbSNP:759924404
1157 1157 c, t dbSNP:547532857
1159 1159 -, a dbSNP:387906283
1174 1174 g, t dbSNP:559365204
1175 1175 c, t dbSNP:756978324
1177 1177 a, g dbSNP:778512952
1182 1182 c, t dbSNP:749931948
1189 1189 c, t dbSNP:757908194
1192 1192 a, g dbSNP:780007425
1200 1200 a, g dbSNP:373771053
1207 1207 g, t dbSNP:751682437
1212 1212 g, t dbSNP:120074143
1214 1214 a, g dbSNP:120074140
1218 1218 c, t dbSNP:768448235
1235 1235 a, g dbSNP:780994047
1236 1236 a, c, t dbSNP:748303093
1239 1239 c, g dbSNP:773491386
1243 1243 a, g dbSNP:377295639
1248 1248 c, g dbSNP:371070695
1257 1257 c, t dbSNP:771006357
1264 1264 c, t dbSNP:774301980
1265 1265 a, c dbSNP:746332363
1271 1271 a, g dbSNP:772696101
1274 1274 c, t dbSNP:201183545
1275 1275 a, g dbSNP:761086326
1276 1276 c, t dbSNP:748749897
1278 1278 c, t dbSNP:764599801
1285 1285 a, g dbSNP:200729208
1292 1292 c, g dbSNP:762543361
1293 1293 a, g dbSNP:147872303
1297 1297 c, t dbSNP:367713788
1298 1298 a, g dbSNP:553101581
1305 1305 c, t dbSNP:767412638
1306 1306 c, t dbSNP:189339614
1307 1307 a, g dbSNP:755855638
1319 1319 -, gaggagga dbSNP:763847352
1323 1323 a, g dbSNP:777462355
1337 1337 a, g dbSNP:749463321
1351 1351 c, g dbSNP:757435663
1355 1355 a, c dbSNP:201500937
1358 1358 -, agac dbSNP:753615294
1364 1364 -, c dbSNP:111390656
1365 1365 c, t dbSNP:745942477
1366 1366 a, c dbSNP:772315169
1388 1388 c, t dbSNP:370288623
1390 1390 g, t dbSNP:747537829
1399 1399 -, aga dbSNP:750365525
1404 1404 a, g dbSNP:772007911
1408 1408 c, g dbSNP:778671746
1411 1411 a, g dbSNP:776862137
1412 1412 -, aatc dbSNP:758479564
1424 1424 a, c dbSNP:747994310
1474 1474 g, t dbSNP:373499678
1480 1480 -, ttaac dbSNP:556749538
1497 1497 a, t dbSNP:192326657
1500 1500 -, atg dbSNP:745386184
1518 1518 a, g dbSNP:771809384
1528 1528 -, taaaaa dbSNP:201268334
1539 1539 -, ttc dbSNP:372218361
1539 1539 g, t dbSNP:114728153
1544 1544 c, t dbSNP:773032425
1550 1550 c, g dbSNP:528994308
1574 1574 c, g dbSNP:145690535
1603 1603 c, t dbSNP:148934438
1610 1610 c, t dbSNP:79179095
1611 1611 a, g dbSNP:542328058
1618 1618 a, g dbSNP:79764488
1651 1651 a, c dbSNP:771132416
1653 1653 a, t dbSNP:73559273
1704 1704 c, t dbSNP:776705661
1715 1715 c, g dbSNP:114039574
1717 1717 a, c dbSNP:373983292
1725 1725 c, t dbSNP:145398051
1757 1757 c, t dbSNP:568860814
1775 1775 c, t dbSNP:760462304
1780 1780 a, t dbSNP:569215583
1825 1825 -, aatc dbSNP:537463051
1867 1867 a, g dbSNP:12271846
1913 1913 a, t dbSNP:183999318
1915 1915 a, g dbSNP:111376037
1934 1934 c, g dbSNP:775813923
1959 1959 a, c dbSNP:2280332
1980 1980 c, t dbSNP:534079630
1997 1997 a, g dbSNP:116471564
2050 2050 c, t dbSNP:574706448
2063 2063 a, t dbSNP:188826374
2067 2067 c, t dbSNP:181581446
2068 2068 a, g dbSNP:575165263
2096 2096 c, t dbSNP:776479087
2103 2103 -, ag dbSNP:563995916
2104 2104 -, ga dbSNP:373601338
2107 2107 -, tatc dbSNP:773677466
2113 2113 c, t dbSNP:186239800
2114 2114 a, g dbSNP:147695530

Target ORF information:

RefSeq Version NM_000019
Organism Homo sapiens (human)
Definition Homo sapiens acetyl-CoA acetyltransferase 1 (ACAT1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000019

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCTGTGC TGGCGGCACT TCTGCGCAGC GGCGCCCGCA GCCGCAGCCC CCTGCTCCGG 
AGGCTGGTGC AGGAAATAAG ATATGTGGAA CGGAGTTATG TATCAAAACC CACTTTGAAG
GAAGTGGTCA TAGTAAGTGC TACAAGAACA CCCATTGGAT CTTTTTTAGG CAGCCTTTCC
TTGCTGCCAG CCACTAAGCT TGGTTCCATT GCAATTCAGG GAGCCATTGA AAAGGCAGGG
ATTCCAAAAG AAGAAGTGAA AGAAGCATAC ATGGGTAATG TTCTACAAGG AGGTGAAGGA
CAAGCTCCTA CAAGGCAGGC AGTATTGGGT GCAGGCTTAC CTATTTCTAC TCCATGTACC
ACCATAAACA AAGTTTGTGC TTCAGGAATG AAAGCCATCA TGATGGCCTC TCAAAGTCTT
ATGTGTGGAC ATCAGGATGT GATGGTGGCA GGTGGGATGG AGAGCATGTC CAATGTTCCA
TATGTAATGA ACAGAGGATC AACACCATAT GGTGGGGTAA AGCTTGAAGA TTTGATTGTA
AAAGACGGGC TAACTGATGT CTACAATAAA ATTCATATGG GCAGCTGTGC TGAGAATACA
GCAAAGAAGC TGAATATTGC ACGAAATGAA CAGGACGCTT ATGCTATTAA TTCTTATACC
AGAAGTAAAG CAGCATGGGA AGCTGGGAAA TTTGGAAATG AAGTTATTCC TGTCACAGTT
ACAGTAAAAG GTCAACCAGA TGTAGTGGTG AAAGAAGATG AAGAATATAA ACGTGTTGAT
TTTAGCAAAG TTCCAAAGCT GAAGACAGTT TTCCAGAAAG AAAATGGCAC AGTAACAGCT
GCCAATGCCA GTACACTGAA TGATGGAGCA GCTGCTCTGG TTCTCATGAC GGCAGATGCA
GCGAAGAGGC TCAATGTTAC ACCACTGGCA AGAATAGTAG CATTTGCTGA CGCTGCTGTA
GAACCTATTG ATTTTCCAAT TGCTCCTGTA TATGCTGCAT CTATGGTTCT TAAAGATGTG
GGATTGAAAA AAGAAGATAT TGCAATGTGG GAAGTAAATG AAGCCTTTAG TCTGGTTGTA
CTAGCAAACA TTAAAATGTT GGAGATTGAT CCCCAAAAAG TGAATATCAA TGGAGGAGCT
GTTTCTCTGG GACATCCAAT TGGGATGTCT GGAGCCAGGA TTGTTGGTCA TTTGACTCAT
GCCTTGAAGC AAGGAGAATA CGGTCTTGCC AGTATTTGCA ATGGAGGAGG AGGTGCTTCT
GCCATGCTAA TTCAGAAGCT GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.