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ARF5 cDNA ORF clone, Homo sapiens (human)

Gene Symbol ARF5
Entrez Gene ID 381
Full Name ADP-ribosylation factor 5
General protein information
Preferred Names
ADP-ribosylation factor 5
Names
ADP-ribosylation factor 5
NP_001653.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7q31.3

Summary This gene is a member of the human ADP-ribosylation factor (ARF) gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. [provided by RefSeq, Dec 2010]. lac of sum
Disorder MIM:

103188


mRNA and Protein(s)

mRNA Protein Name
NM_001662 NP_001653 ADP-ribosylation factor 5



Homo sapiens (human) ARF5 NP_001653.1
Pan troglodytes (chimpanzee) ARF5 XP_519351.3
Canis lupus familiaris (dog) ARF5 XP_532438.3
Bos taurus (cattle) ARF5 NP_001077140.1
Mus musculus (house mouse) Arf5 NP_031506.1
Rattus norvegicus (Norway rat) Arf5 NP_077063.1
Danio rerio (zebrafish) arf5 NP_954969.1
Danio rerio (zebrafish) zgc:77650 NP_956170.1
Caenorhabditis elegans arf-3 NP_501336.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ARF5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARF5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu01577
NM_001662 Homo sapiens ADP-ribosylation factor 5 (ARF5), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu01577
Clone ID Related Accession (Same CDS sequence) NM_001662
Accession Version NM_001662.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 543bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ADP-ribosylation factor 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN259625.1 and AC000357.1. On Dec 9, 2010 this sequence version replaced gi:6995999. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC033104.1, AL545339.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
ATGGGCCTCA CCGTGTCCGC GCTCTTTTCG CGGATCTTCG GGAAGAAGCA GATGCGGATT 
CTCATGGTTG GCTTGGATGC GGCTGGCAAG ACCACAATCC TGTACAAACT GAAGTTGGGG
GAGATTGTCA CCACCATCCC AACCATAGGC TTCAATGTAG AAACAGTGGA ATATAAGAAC
ATCTGTTTCA CAGTCTGGGA CGTGGGAGGC CAGGACAAGA TTCGGCCTCT GTGGCGGCAC
TACTTCCAGA ACACTCAGGG CCTCATCTTT GTGGTGGACA GTAATGACCG GGAGCGGGTC
CAAGAATCTG CTGATGAACT CCAGAAGATG CTGCAGGAGG ACGAGCTGCG GGATGCAGTG
CTGCTGGTAT TTGCCAACAA GCAGGACATG CCCAACGCCA TGCCCGTGAG CGAGCTGACT
GACAAGCTGG GGCTACAGCA CTTACGCAGC CGCACGTGGT ATGTCCAGGC CACCTGTGCC
ACCCAAGGCA CAGGTCTGTA CGATGGTCTG GACTGGCTGT CCCACGAGCT GTCAAAGCGC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001653.1
CDS148..690
Misc Feature(1)184..>618(+)
Misc Feature(2)199..675(+)
Misc Feature(3)217..240(+)
Misc Feature(4)223..630(+)
Misc Feature(5)223..390(+)
Misc Feature(6)253..300(+)
Misc Feature(7)289..390(+)
Misc Feature(8)289..291(+)
Misc Feature(9)292..387(+)
Misc Feature(10)298..345(+)
Misc Feature(11)346..357(+)
Misc Feature(12)352..405(+)
Misc Feature(13)523..534(+)
Misc Feature(14)622..630(+)
Exon (1)1..214
Gene:ARF5
Gene Synonym:
Exon (2)215..295
Gene:ARF5
Gene Synonym:
Exon (3)296..405
Gene:ARF5
Gene Synonym:
Exon (4)406..477
Gene:ARF5
Gene Synonym:
Exon (5)478..603
Gene:ARF5
Gene Synonym:
Exon (6)604..1096
Gene:ARF5
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001662
Organism Homo sapiens (human)
Definition Homo sapiens ADP-ribosylation factor 5 (ARF5), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001662

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGCCTCA CCGTGTCCGC GCTCTTTTCG CGGATCTTCG GGAAGAAGCA GATGCGGATT 
CTCATGGTTG GCTTGGATGC GGCTGGCAAG ACCACAATCC TGTACAAACT GAAGTTGGGG
GAGATTGTCA CCACCATCCC AACCATAGGC TTCAATGTAG AAACAGTGGA ATATAAGAAC
ATCTGTTTCA CAGTCTGGGA CGTGGGAGGC CAGGACAAGA TTCGGCCTCT GTGGCGGCAC
TACTTCCAGA ACACTCAGGG CCTCATCTTT GTGGTGGACA GTAATGACCG GGAGCGGGTC
CAAGAATCTG CTGATGAACT CCAGAAGATG CTGCAGGAGG ACGAGCTGCG GGATGCAGTG
CTGCTGGTAT TTGCCAACAA GCAGGACATG CCCAACGCCA TGCCCGTGAG CGAGCTGACT
GACAAGCTGG GGCTACAGCA CTTACGCAGC CGCACGTGGT ATGTCCAGGC CACCTGTGCC
ACCCAAGGCA CAGGTCTGTA CGATGGTCTG GACTGGCTGT CCCACGAGCT GTCAAAGCGC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Genome-wide association study in a Chinese population identifies a susceptibility locus for type 2 diabetes at 7q32 near PAX4
Diabetologia 56 (6), 1291-1305 (2013)
Ma RC, Hu C, Tam CH, Zhang R, Kwan P, Leung TF, Thomas GN, Go MJ, Hara K, Sim X, Ho JS, Wang C, Li H, Lu L, Wang Y, Li JW, Wang Y, Lam VK, Wang J, Yu W, Kim YJ, Ng DP, Fujita H, Panoutsopoulou K, Day-Williams AG, Lee HM, Ng AC, Fang YJ, Kong AP, Jiang F, Ma X, Hou X, Tang S, Lu J, Yamauchi T, Tsui SK, Woo J, Leung PC, Zhang X, Tang NL, Sy HY, Liu J, Wong TY, Lee JY, Maeda S, Xu G, Cherny SS, Chan TF, Ng MC, Xiang K, Morris AP, Keildson S, Hu R, Ji L, Lin X, Cho YS, Kadowaki T, Tai ES, Zeggini E, McCarthy MI, Hon KL, Baum L, Tomlinson B, So WY, Bao Y, Chan JC and Jia W.


book

BRAG2/GEP100/IQSec1 interacts with clathrin and regulates alpha5beta1 integrin endocytosis through activation of ADP ribosylation factor 5 (Arf5)
J. Biol. Chem. 287 (37), 31138-31147 (2012)
Moravec R, Conger KK, D'Souza R, Allison AB and Casanova JE.


book

ARAP1 regulates the ring size of circular dorsal ruffles through Arf1 and Arf5
Mol. Biol. Cell 23 (13), 2481-2489 (2012)
Hasegawa J, Tsujita K, Takenawa T and Itoh T.


book

Proteomic analysis identifies dysfunction in cellular transport, energy, and protein metabolism in different brain regions of atypical frontotemporal lobar degeneration
J. Proteome Res. 11 (4), 2533-2543 (2012)
Martins-de-Souza D, Guest PC, Mann DM, Roeber S, Rahmoune H, Bauder C, Kretzschmar H, Volk B, Baborie A and Bahn S.


book

GBF1-Arf-COPI-ArfGAP-mediated Golgi-to-ER transport involved in regulation of lipid homeostasis
Cell Struct. Funct. 36 (2), 223-235 (2011)
Takashima K, Saitoh A, Hirose S, Nakai W, Kondo Y, Takasu Y, Kakeya H, Shin HW and Nakayama K.


book

Coated vesicle assembly in the Golgi requires only coatomer and ARF proteins from the cytosol
Nature 364 (6439), 732-734 (1993)
Orcl L, Palmer DJ, Amherdt M and Rothman JE.


book

Two distinct populations of ARF bound to Golgi membranes
J. Cell Biol. 121 (4), 751-760 (1993)
Helms JB, Palmer DJ and Rothman JE.


book

Human and Giardia ADP-ribosylation factors (ARFs) complement ARF function in Saccharomyces cerevisiae
J. Biol. Chem. 267 (34), 24441-24445 (1992)
Lee FJ, Moss J and Vaughan M.


book

Molecular identification of ADP-ribosylation factor mRNAs and their expression in mammalian cells
J. Biol. Chem. 266 (5), 2772-2777 (1991)
Tsuchiya M, Price SR, Tsai SC, Moss J and Vaughan M.


book

ADP-ribosylation factor is functionally and physically associated with the Golgi complex
Proc. Natl. Acad. Sci. U.S.A. 87 (3), 1238-1242 (1990)
Stearns T, Willingham MC, Botstein D and Kahn RA.


 
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