×

KRT6B cDNA ORF clone, Homo sapiens (human)

Gene Symbol KRT6B
Entrez Gene ID 3854
Full Name keratin 6B, type II
Synonyms CK-6B, CK6B, K6B, KRTL1, PC2, PC4
General protein information
Preferred Names
keratin, type II cytoskeletal 6B
Names
keratin, type II cytoskeletal 6B
keratin-6B
cytokeratin 6B
cytokeratin-6B
type-II keratin Kb10
keratin, epidermal, type II, K6B
keratin-like 1 (a type II keratin sequence)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12q13.13

Summary The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. Mutations in these genes have been associated with pachyonychia congenita. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

148042

Disorder Html: Pachyonychia congenita, Jackson-Lawler type, 167210 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_005555 NP_005546 keratin, type II cytoskeletal 6B


Homo sapiens (human) KRT6B NP_005546.2
Pan troglodytes (chimpanzee) KRT6C XP_001143340.1
Mus musculus (house mouse) Krt6a NP_032502.3
Mus musculus (house mouse) Krt6b NP_034799.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following KRT6B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KRT6B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu17785
NM_005555 Homo sapiens keratin 6B, type II (KRT6B), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu17785
Clone ID Related Accession (Same CDS sequence) NM_005555
Accession Version NM_005555.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1695bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product keratin, type II cytoskeletal 6B
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Michael Rogers. The reference sequence was derived from BC034535.1 and BG679927.1. This sequence is a reference standard in the RefSeqGene project. On Dec 20, 2006 this sequence version replaced gi:17505187. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC034535.1, L42612.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147975, SAMEA2156670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCCAGCA CATCCACCAC CATCAGGAGC CACAGCAGCA GCCGCCGGGG TTTCAGTGCC 
AACTCAGCCA GGCTCCCTGG GGTCAGCCGC TCTGGCTTCA GCAGCATCTC CGTGTCCCGC
TCCAGGGGCA GTGGTGGCCT GGGTGGCGCA TGTGGAGGAG CTGGCTTTGG CAGCCGCAGT
CTGTATGGCC TGGGGGGCTC CAAGAGGATC TCCATTGGAG GGGGCAGCTG TGCCATCAGT
GGCGGCTATG GCAGCAGAGC CGGAGGCAGC TATGGCTTTG GTGGCGCCGG GAGTGGATTT
GGTTTCGGTG GTGGAGCCGG CATTGGCTTT GGTCTGGGTG GTGGAGCCGG CCTTGCTGGT
GGCTTTGGGG GCCCTGGCTT CCCTGTGTGC CCCCCTGGAG GCATCCAAGA GGTCACTGTC
AACCAGAGTC TCCTGACTCC CCTCAACCTG CAAATTGACC CCGCCATCCA GCGGGTGCGG
GCCGAGGAGC GTGAGCAGAT CAAGACCCTC AACAACAAGT TTGCCTCCTT CATCGACAAG
GTGCGGTTCC TAGAGCAGCA GAACAAGGTT CTGGACACCA AGTGGACCCT GCTGCAGGAG
CAGGGCACCA AGACTGTGAG GCAGAACCTG GAGCCGTTGT TCGAGCAGTA CATCAACAAC
CTCAGGAGGC AGCTGGACAA CATCGTGGGG GAACGGGGTC GTCTGGACTC GGAGCTGAGA
AACATGCAGG ACCTGGTGGA GGACCTCAAG AACAAATATG AGGATGAAAT CAACAAGCGC
ACAGCAGCAG AGAATGAATT TGTGACTCTG AAGAAGGATG TGGATGCTGC CTACATGAAC
AAGGTTGAAC TGCAAGCCAA GGCAGACACT CTTACAGATG AGATCAACTT CCTGAGAGCC
TTGTATGATG CAGAGCTGTC CCAGATGCAG ACCCACATCT CAGACACATC CGTGGTGCTA
TCCATGGACA ACAACCGCAA CCTGGACCTG GACAGCATCA TCGCTGAGGT CAAGGCCCAA
TATGAGGAGA TTGCTCAGAG GAGCAGGGCT GAGGCTGAGT CCTGGTACCA GACAAAGTAC
GAGGAGCTGC AGATCACAGC AGGCAGACAT GGGGACGACC TGCGCAACAC CAAGCAGGAG
ATTGCTGAGA TCAACCGCAT GATCCAGAGG CTGAGATCTG AGATCGACCA CGTCAAGAAG
CAGTGTGCCA ACCTACAGGC CGCCATTGCT GATGCTGAGC AGCGTGGGGA GATGGCCCTC
AAGGATGCTA AGAACAAGCT GGAAGGGCTG GAGGATGCCC TGCAGAAGGC CAAGCAGGAC
CTGGCCCGGC TGCTGAAGGA GTACCAGGAG CTGATGAACG TCAAGCTGGC CCTGGATGTG
GAGATCGCCA CCTACCGCAA GCTGCTGGAG GGCGAGGAGT GCAGGCTGAA TGGCGAAGGC
GTTGGACAAG TCAACATCTC TGTAGTGCAG TCCACCGTCT CCAGTGGCTA TGGCGGTGCC
AGCGGTGTCG GCAGTGGCTT AGGCCTGGGT GGAGGAAGCA GCTACTCCTA TGGCAGTGGT
CTTGGCGTTG GAGGCGGCTT TAGTTCCAGC AGCGGCAGAG CCACTGGGGG TGGCCTCAGC
TCTGTTGGAG GCGGCAGTTC CACCATCAAG TACACCACCA CCTCCTCCTC CAGCAGGAAG
AGCTACAAGC ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005546.2
CDS49..1743
Misc Feature(1)25..27(+)
Misc Feature(2)52..534(+)
Misc Feature(3)52..54(+)
Misc Feature(4)226..228(+)
Misc Feature(5)532..1473(+)
Misc Feature(6)535..1464(+)
Misc Feature(7)535..642(+)
Misc Feature(8)643..699(+)
Misc Feature(9)700..975(+)
Misc Feature(10)976..1047(+)
Misc Feature(11)1048..1464(+)
Misc Feature(12)1156..>1491(+)
Misc Feature(13)1288..1290(+)
Misc Feature(14)1465..1740(+)
Exon (1)1..588
Gene:KRT6B
Gene Synonym:
Exon (2)589..803
Gene:KRT6B
Gene Synonym:
Exon (3)804..864
Gene:KRT6B
Gene Synonym:
Exon (4)865..960
Gene:KRT6B
Gene Synonym:
Exon (5)961..1125
Gene:KRT6B
Gene Synonym:
Exon (6)1126..1251
Gene:KRT6B
Gene Synonym:
Exon (7)1252..1472
Gene:KRT6B
Gene Synonym:
Exon (8)1473..1507
Gene:KRT6B
Gene Synonym:
Exon (9)1508..2282
Gene:KRT6B
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_005555
Organism Homo sapiens (human)
Definition Homo sapiens keratin 6B, type II (KRT6B), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005555

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCCAGCA CATCCACCAC CATCAGGAGC CACAGCAGCA GCCGCCGGGG TTTCAGTGCC 
AACTCAGCCA GGCTCCCTGG GGTCAGCCGC TCTGGCTTCA GCAGCATCTC CGTGTCCCGC
TCCAGGGGCA GTGGTGGCCT GGGTGGCGCA TGTGGAGGAG CTGGCTTTGG CAGCCGCAGT
CTGTATGGCC TGGGGGGCTC CAAGAGGATC TCCATTGGAG GGGGCAGCTG TGCCATCAGT
GGCGGCTATG GCAGCAGAGC CGGAGGCAGC TATGGCTTTG GTGGCGCCGG GAGTGGATTT
GGTTTCGGTG GTGGAGCCGG CATTGGCTTT GGTCTGGGTG GTGGAGCCGG CCTTGCTGGT
GGCTTTGGGG GCCCTGGCTT CCCTGTGTGC CCCCCTGGAG GCATCCAAGA GGTCACTGTC
AACCAGAGTC TCCTGACTCC CCTCAACCTG CAAATTGACC CCGCCATCCA GCGGGTGCGG
GCCGAGGAGC GTGAGCAGAT CAAGACCCTC AACAACAAGT TTGCCTCCTT CATCGACAAG
GTGCGGTTCC TAGAGCAGCA GAACAAGGTT CTGGACACCA AGTGGACCCT GCTGCAGGAG
CAGGGCACCA AGACTGTGAG GCAGAACCTG GAGCCGTTGT TCGAGCAGTA CATCAACAAC
CTCAGGAGGC AGCTGGACAA CATCGTGGGG GAACGGGGTC GTCTGGACTC GGAGCTGAGA
AACATGCAGG ACCTGGTGGA GGACCTCAAG AACAAATATG AGGATGAAAT CAACAAGCGC
ACAGCAGCAG AGAATGAATT TGTGACTCTG AAGAAGGATG TGGATGCTGC CTACATGAAC
AAGGTTGAAC TGCAAGCCAA GGCAGACACT CTTACAGATG AGATCAACTT CCTGAGAGCC
TTGTATGATG CAGAGCTGTC CCAGATGCAG ACCCACATCT CAGACACATC CGTGGTGCTA
TCCATGGACA ACAACCGCAA CCTGGACCTG GACAGCATCA TCGCTGAGGT CAAGGCCCAA
TATGAGGAGA TTGCTCAGAG GAGCAGGGCT GAGGCTGAGT CCTGGTACCA GACAAAGTAC
GAGGAGCTGC AGATCACAGC AGGCAGACAT GGGGACGACC TGCGCAACAC CAAGCAGGAG
ATTGCTGAGA TCAACCGCAT GATCCAGAGG CTGAGATCTG AGATCGACCA CGTCAAGAAG
CAGTGTGCCA ACCTACAGGC CGCCATTGCT GATGCTGAGC AGCGTGGGGA GATGGCCCTC
AAGGATGCTA AGAACAAGCT GGAAGGGCTG GAGGATGCCC TGCAGAAGGC CAAGCAGGAC
CTGGCCCGGC TGCTGAAGGA GTACCAGGAG CTGATGAACG TCAAGCTGGC CCTGGATGTG
GAGATCGCCA CCTACCGCAA GCTGCTGGAG GGCGAGGAGT GCAGGCTGAA TGGCGAAGGC
GTTGGACAAG TCAACATCTC TGTAGTGCAG TCCACCGTCT CCAGTGGCTA TGGCGGTGCC
AGCGGTGTCG GCAGTGGCTT AGGCCTGGGT GGAGGAAGCA GCTACTCCTA TGGCAGTGGT
CTTGGCGTTG GAGGCGGCTT TAGTTCCAGC AGCGGCAGAG CCACTGGGGG TGGCCTCAGC
TCTGTTGGAG GCGGCAGTTC CACCATCAAG TACACCACCA CCTCCTCCTC CAGCAGGAAG
AGCTACAAGC ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A review of the clinical phenotype of 254 patients with genetically confirmed pachyonychia congenita
J. Am. Acad. Dermatol. 67 (4), 680-686 (2012)
Eliason MJ, Leachman SA, Feng BJ, Schwartz ME and Hansen CD.


book

The diagnostic utility of D2-40, calretinin, CK5/6, desmin and MOC-31 in the differentiation of mesothelioma from adenocarcinoma in pleural effusion cytology
Acta Cytol. 56 (5), 527-532 (2012)
Hyun TS, Barnes M and Tabatabai ZL.


book

Prediction of local recurrence, distant metastases, and death after breast-conserving therapy in early-stage invasive breast cancer using a five-biomarker panel
J. Clin. Oncol. 27 (28), 4701-4708 (2009)
Millar EK, Graham PH, O'Toole SA, McNeil CM, Browne L, Morey AL, Eggleton S, Beretov J, Theocharous C, Capp A, Nasser E, Kearsley JH, Delaney G, Papadatos G, Fox C and Sutherland RL.


book

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT)
J. Proteome Res. 8 (3), 1304-1314 (2009)
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE and Yates JR.


book

New consensus nomenclature for mammalian keratins
J. Cell Biol. 174 (2), 169-174 (2006)
Schweizer J, Bowden PE, Coulombe PA, Langbein L, Lane EB, Magin TM, Maltais L, Omary MB, Parry DA, Rogers MA and Wright MW.


book

Pachyonychia Congenita
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Smith,F.J.D., Hansen,C.D., Hull,P.R., Kaspar,R.L., Schwartz,M.E., McLean,W.H.I., Milstone,L.M., van Steensel,M., O'Toole,E. and Sprecher,E.


book

Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes
Electrophoresis 13 (12), 960-969 (1992)
Rasmussen HH, van Damme J, Puype M, Gesser B, Celis JE and Vandekerckhove J.


book

Squamous cell carcinomas, an immunohistochemical and ultrastructural study
Anticancer Res. 12 (6B), 2025-2031 (1992)
Suo Z, Holm R and Nesland JM.


book

Three epidermal and one simple epithelial type II keratin genes map to human chromosome 12
Cytogenet. Cell Genet. 57 (1), 33-38 (1991)
Rosenberg M, Fuchs E, Le Beau MM, Eddy RL and Shows TB.


book

The sequence of a type II keratin gene expressed in human skin: conservation of structure among all intermediate filament genes
Proc. Natl. Acad. Sci. U.S.A. 82 (14), 4683-4687 (1985)
Tyner,A.L., Eichman,M.J. and Fuchs,E.


 
*
*
*
*