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KRT12 cDNA ORF clone, Homo sapiens (human)

Gene Symbol KRT12
Entrez Gene ID 3859
Full Name keratin 12, type I
Synonyms K12
General protein information
Preferred Names
keratin, type I cytoskeletal 12
Names
keratin, type I cytoskeletal 12
CK-12
keratin-12
cytokeratin-12
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q12

Summary KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

601687

Disorder Html: Meesmann corneal dystrophy, 122100 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000223 NP_000214 keratin, type I cytoskeletal 12


Homo sapiens (human) KRT12 NP_000214.1
Pan troglodytes (chimpanzee) KRT12 XP_001168468.1
Macaca mulatta (Rhesus monkey) KRT12 XP_001101582.1
Canis lupus familiaris (dog) KRT12 NP_001075890.1
Bos taurus (cattle) KRT12 XP_002696056.2
Mus musculus (house mouse) Krt12 NP_034791.2
Rattus norvegicus (Norway rat) Krt12 NP_001008761.1
Gallus gallus (chicken) KRT12 XP_004948663.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC100486967 XP_002940696.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following KRT12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KRT12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18523
NM_000223 Homo sapiens keratin 12, type I (KRT12), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$204.50-$286.30
$409.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18523
Clone ID Related Accession (Same CDS sequence) NM_000223
Accession Version NM_000223.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1485bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product keratin, type I cytoskeletal 12
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D78367.1 and CV573989.1. This sequence is a reference standard in the RefSeqGene project. On Aug 29, 2007 this sequence version replaced gi:47132618. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D78367.1, AK313747.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGATCTCT CCAACAACAC CATGTCACTC TCAGTGCGCA CCCCCGGACT GTCCCGGCGG 
CTCTCCTCGC AGAGTGTGAT AGGCAGACCC AGGGGCATGT CTGCTTCCAG TGTTGGAAGT
GGTTATGGGG GAAGTGCCTT TGGCTTTGGA GCCAGCTGTG GGGGAGGCTT TTCTGCTGCT
TCCATGTTTG GTTCTAGTTC CGGCTTTGGG GGTGGCTCCG GAAGTTCCAT GGCAGGAGGA
CTGGGTGCTG GTTATGGGAG AGCCCTGGGT GGAGGTAGCT TTGGAGGGCT GGGGATGGGA
TTTGGGGGCA GCCCAGGAGG TGGCTCTCTA GGTATTCTCT CGGGCAATGA TGGAGGCCTT
CTTTCTGGAT CAGAAAAAGA AACTATGCAA AATCTTAATG ATAGATTAGC TTCCTACCTG
GATAAGGTGC GAGCTCTAGA AGAGGCTAAT ACTGAGCTAG AAAATAAAAT TCGAGAATGG
TATGAAACAC GAGGAACTGG GACTGCAGAT GCTTCACAGA GCGATTACAG CAAATATTAT
CCACTGATTG AAGACCTCAG GAATAAGATC ATTTCAGCCA GCATTGGAAA TGCCCAGCTC
CTCTTGCAGA TTGACAATGC GAGACTAGCT GCTGAGGACT TCAGGATGAA GTATGAGAAT
GAACTGGCCC TGCGCCAGGG CGTAGAGGCC GACATCAATG GCCTGCGCCG GGTGCTGGAC
GAGCTGACCC TGACCAGGAC CGACCTGGAG ATGCAGATCG AGAGCCTGAA CGAGGAGCTG
GCCTACATGA AGAAGAACCA CGAGGATGAG CTCCAAAGCT TCCGGGTGGG CGGCCCAGGC
GAGGTCAGCG TAGAAATGGA CGCTGCCCCC GGAGTGGACC TCACCAGGCT CCTCAATGAT
ATGCGGGCGC AGTATGAAAC CATCGCTGAG CAGAATCGGA AGGACGCTGA AGCCTGGTTC
ATTGAAAAGA GCGGGGAGCT CCGTAAGGAG ATTAGCACCA ACACCGAGCA GCTTCAGTCC
AGCAAGAGCG AGGTCACCGA CCTGCGTCGC GCCTTTCAGA ACCTGGAGAT CGAGCTACAG
TCCCAGCTCG CCATGAAGAA ATCCCTGGAG GACTCCTTGG CCGAAGCCGA GGGCGATTAC
TGCGCGCAGC TGTCCCAGGT GCAGCAGCTC ATCAGCAACC TGGAGGCACA GCTGCTCCAG
GTGCGCGCGG ACGCAGAGCG CCAGAACGTG GACCACCAGC GGCTGCTGAA TGTCAAGGCC
CGCCTGGAGC TGGAGATTGA GACCTACCGC CGCCTGCTGG ACGGGGAGGC CCAAGGTGAT
GGTTTGGAGG AAAGTTTATT TGTGACAGAC TCCAAATCAC AAGCACAGTC AACTGATTCC
TCTAAAGACC CAACCAAAAC CCGAAAAATC AAGACAGTTG TGCAGGAGAT GGTGAATGGT
GAGGTGGTCT CATCTCAAGT TCAGGAAATT GAAGAACTAA TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000214.1
CDS25..1509
Misc Feature(1)25..396(+)
Misc Feature(2)394..1332(+)
Misc Feature(3)397..1329(+)
Misc Feature(4)397..504(+)
Misc Feature(5)514..570(+)
Misc Feature(6)571..846(+)
Misc Feature(7)847..915(+)
Misc Feature(8)916..1329(+)
Misc Feature(9)1330..1506(+)
Exon (1)1..591
Gene:KRT12
Gene Synonym:
Exon (2)592..674
Gene:KRT12
Gene Synonym:
Exon (3)675..831
Gene:KRT12
Gene Synonym:
Exon (4)832..993
Gene:KRT12
Gene Synonym:
Exon (5)994..1119
Gene:KRT12
Gene Synonym:
Exon (6)1120..1340
Gene:KRT12
Gene Synonym:
Exon (7)1341..1411
Gene:KRT12
Gene Synonym:
Exon (8)1412..1992
Gene:KRT12
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000223
Organism Homo sapiens (human)
Definition Homo sapiens keratin 12, type I (KRT12), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000223

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGATCTCT CCAACAACAC CATGTCACTC TCAGTGCGCA CCCCCGGACT GTCCCGGCGG 
CTCTCCTCGC AGAGTGTGAT AGGCAGACCC AGGGGCATGT CTGCTTCCAG TGTTGGAAGT
GGTTATGGGG GAAGTGCCTT TGGCTTTGGA GCCAGCTGTG GGGGAGGCTT TTCTGCTGCT
TCCATGTTTG GTTCTAGTTC CGGCTTTGGG GGTGGCTCCG GAAGTTCCAT GGCAGGAGGA
CTGGGTGCTG GTTATGGGAG AGCCCTGGGT GGAGGTAGCT TTGGAGGGCT GGGGATGGGA
TTTGGGGGCA GCCCAGGAGG TGGCTCTCTA GGTATTCTCT CGGGCAATGA TGGAGGCCTT
CTTTCTGGAT CAGAAAAAGA AACTATGCAA AATCTTAATG ATAGATTAGC TTCCTACCTG
GATAAGGTGC GAGCTCTAGA AGAGGCTAAT ACTGAGCTAG AAAATAAAAT TCGAGAATGG
TATGAAACAC GAGGAACTGG GACTGCAGAT GCTTCACAGA GCGATTACAG CAAATATTAT
CCACTGATTG AAGACCTCAG GAATAAGATC ATTTCAGCCA GCATTGGAAA TGCCCAGCTC
CTCTTGCAGA TTGACAATGC GAGACTAGCT GCTGAGGACT TCAGGATGAA GTATGAGAAT
GAACTGGCCC TGCGCCAGGG CGTAGAGGCC GACATCAATG GCCTGCGCCG GGTGCTGGAC
GAGCTGACCC TGACCAGGAC CGACCTGGAG ATGCAGATCG AGAGCCTGAA CGAGGAGCTG
GCCTACATGA AGAAGAACCA CGAGGATGAG CTCCAAAGCT TCCGGGTGGG CGGCCCAGGC
GAGGTCAGCG TAGAAATGGA CGCTGCCCCC GGAGTGGACC TCACCAGGCT CCTCAATGAT
ATGCGGGCGC AGTATGAAAC CATCGCTGAG CAGAATCGGA AGGACGCTGA AGCCTGGTTC
ATTGAAAAGA GCGGGGAGCT CCGTAAGGAG ATTAGCACCA ACACCGAGCA GCTTCAGTCC
AGCAAGAGCG AGGTCACCGA CCTGCGTCGC GCCTTTCAGA ACCTGGAGAT CGAGCTACAG
TCCCAGCTCG CCATGAAGAA ATCCCTGGAG GACTCCTTGG CCGAAGCCGA GGGCGATTAC
TGCGCGCAGC TGTCCCAGGT GCAGCAGCTC ATCAGCAACC TGGAGGCACA GCTGCTCCAG
GTGCGCGCGG ACGCAGAGCG CCAGAACGTG GACCACCAGC GGCTGCTGAA TGTCAAGGCC
CGCCTGGAGC TGGAGATTGA GACCTACCGC CGCCTGCTGG ACGGGGAGGC CCAAGGTGAT
GGTTTGGAGG AAAGTTTATT TGTGACAGAC TCCAAATCAC AAGCACAGTC AACTGATTCC
TCTAAAGACC CAACCAAAAC CCGAAAAATC AAGACAGTTG TGCAGGAGAT GGTGAATGGT
GAGGTGGTCT CATCTCAAGT TCAGGAAATT GAAGAACTAA TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

siRNA silencing of the mutant keratin 12 allele in corneal limbal epithelial cells grown from patients with Meesmann's epithelial corneal dystrophy
Invest. Ophthalmol. Vis. Sci. 55 (5), 3352-3360 (2014)
Courtney DG, Atkinson SD, Allen EH, Moore JE, Walsh CP, Pedrioli DM, MacEwen CJ, Pellegrini G, Maurizi E, Serafini C, Fantacci M, Liao H, Irvine AD, McLean WH and Moore CB.


book

KRT12 mutations and in vivo confocal microscopy in two Japanese families with Meesmann corneal dystrophy
Am. J. Ophthalmol. 157 (1), 93-102 (2014)
Ogasawara M, Matsumoto Y, Hayashi T, Ohno K, Yamada H, Kawakita T, Dogru M, Shimazaki J, Tsubota K and Tsuneoka H.


book

Autosomal-dominant Meesmann epithelial corneal dystrophy without an exon mutation in the keratin-3 or keratin-12 gene in a Chinese family
J. Int. Med. Res. 41 (2), 511-518 (2013)
Cao W, Yan M, Hao Q, Wang S, Wu L, Liu Q, Li M, Biddle FG and Wu W.


book

Severe Meesmann's epithelial corneal dystrophy phenotype due to a missense mutation in the helix-initiation motif of keratin 12
Eye (Lond) 27 (3), 367-373 (2013)
Hassan H, Thaung C, Ebenezer ND, Larkin G, Hardcastle AJ and Tuft SJ.


book

Allele-specific siRNA silencing for the common keratin 12 founder mutation in Meesmann epithelial corneal dystrophy
Invest. Ophthalmol. Vis. Sci. 54 (1), 494-502 (2013)
Allen EH, Atkinson SD, Liao H, Moore JE, Leslie Pedrioli DM, Smith FJ, McLean WH and Moore CB.


book

A novel keratin 12 mutation in a German kindred with Meesmann's corneal dystrophy
Br J Ophthalmol 84 (5), 527-530 (2000)
Corden LD, Swensson O, Swensson B, Rochels R, Wannke B, Thiel HJ and McLean WH.


book

Molecular genetics of Meesmann's corneal dystrophy: ancestral and novel mutations in keratin 12 (K12) and complete sequence of the human KRT12 gene
Exp. Eye Res. 70 (1), 41-49 (2000)
Corden LD, Swensson O, Swensson B, Smith FJ, Rochels R, Uitto J and McLEAN WH.


book

Isolation and chromosomal localization of a cornea-specific human keratin 12 gene and detection of four mutations in Meesmann corneal epithelial dystrophy
Am. J. Hum. Genet. 61 (6), 1268-1275 (1997)
Nishida K, Honma Y, Dota A, Kawasaki S, Adachi W, Nakamura T, Quantock AJ, Hosotani H, Yamamoto S, Okada M, Shimomura Y and Kinoshita S.


book

Mutations in cornea-specific keratin K3 or K12 genes cause Meesmann's corneal dystrophy
Nat. Genet. 16 (2), 184-187 (1997)
Irvine AD, Corden LD, Swensson O, Swensson B, Moore JE, Frazer DG, Smith FJ, Knowlton RG, Christophers E, Rochels R, Uitto J and McLean WH.


book

A gene expression profile of human corneal epithelium and the isolation of human keratin 12 cDNA
Invest. Ophthalmol. Vis. Sci. 37 (9), 1800-1809 (1996)
Nishida K, Adachi W, Shimizu-Matsumoto A, Kinoshita S, Mizuno K, Matsubara K and Okubo K.


 
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