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KRT16 cDNA ORF clone, Homo sapiens (human)

Gene Symbol KRT16
Entrez Gene ID 3868
Full Name keratin 16, type I
Synonyms CK16, FNEPPK, K16, K1CP, KRT16A, NEPPK, PC1
General protein information
Preferred Names
keratin, type I cytoskeletal 16
Names
keratin, type I cytoskeletal 16
CK-16
keratin-16
cytokeratin 16
cytokeratin-16
focal non-epidermolytic palmoplantar keratoderma
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q21.2

Summary The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

148067

Disorder Html: Pachyonychia congenita, Jadassohn-Lewandowsky type, 167200 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_005557 NP_005548 keratin, type I cytoskeletal 16


Homo sapiens (human) KRT16 NP_005548.2
Pan troglodytes (chimpanzee) KRT16 NP_001138310.1
Macaca mulatta (Rhesus monkey) LOC707236 XP_002800548.1
Canis lupus familiaris (dog) KRT16 XP_548101.2
Bos taurus (cattle) KRT16 XP_003587486.2
Mus musculus (house mouse) Krt16 NP_032496.1
Rattus norvegicus (Norway rat) Krt16 NP_001008752.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following KRT16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KRT16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu24939
NM_005557 Homo sapiens keratin 16, type I (KRT16), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu24939
Clone ID Related Accession (Same CDS sequence) NM_005557
Accession Version NM_005557.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1422bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product keratin, type I cytoskeletal 16
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Michael Rogers. The reference sequence was derived from DC394235.1, AK290853.1 and BC039169.1. This sequence is a reference standard in the RefSeqGene project. On Dec 20, 2007 this sequence version replaced gi:24430191. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC039169.1, AF061812.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGACCACCT GCAGCCGCCA GTTCACCTCC TCCAGCTCCA TGAAGGGCTC CTGCGGCATC 
GGAGGCGGCA TCGGGGGCGG CTCCAGCCGC ATCTCCTCCG TCCTGGCCGG AGGGTCCTGC
CGTGCCCCCA GCACCTACGG GGGCGGCCTG TCTGTCTCCT CTCGCTTCTC CTCTGGGGGA
GCCTGCGGGC TGGGGGGCGG CTATGGCGGT GGCTTCAGCA GCAGCAGCAG CTTTGGTAGT
GGCTTCGGGG GAGGATATGG TGGTGGCCTT GGTGCTGGCT TCGGTGGTGG CTTGGGTGCT
GGCTTTGGTG GTGGTTTTGC TGGTGGTGAT GGGCTTCTGG TGGGCAGTGA GAAGGTGACC
ATGCAGAACC TCAATGACCG CCTGGCCTCC TACCTGGACA AGGTGCGTGC TCTGGAGGAG
GCCAACGCCG ACCTGGAAGT GAAGATCCGT GACTGGTACC AGAGGCAGCG GCCCAGTGAG
ATCAAAGACT ACAGTCCCTA CTTCAAGACC ATCGAGGACC TGAGGAACAA GATCATTGCG
GCCACCATTG AGAATGCGCA GCCCATTTTG CAGATTGACA ATGCCAGGCT GGCAGCCGAT
GACTTCAGGA CCAAGTATGA GCATGAACTG GCCCTGCGGC AGACTGTGGA GGCCGACGTC
AATGGCCTGC GCCGGGTGTT GGATGAGCTG ACCCTGGCCA GGACTGACCT GGAGATGCAG
ATCGAAGGCC TGAAGGAGGA GCTGGCCTAC CTGAGGAAGA ACCACGAGGA GGAGATGCTT
GCTCTGAGAG GTCAGACCGG CGGAGATGTG AACGTGGAGA TGGATGCTGC ACCTGGCGTG
GACCTGAGCC GCATCCTGAA TGAGATGCGT GACCAGTACG AGCAGATGGC AGAGAAAAAC
CGCAGAGACG CTGAGACCTG GTTCCTGAGC AAGACCGAGG AGCTGAACAA AGAAGTGGCC
TCCAACAGCG AACTGGTACA GAGCAGCCGC AGTGAGGTGA CGGAGCTCCG GAGGGTGCTC
CAGGGCCTGG AGATTGAGCT GCAGTCCCAG CTCAGCATGA AAGCATCCCT GGAGAACAGC
CTGGAGGAGA CCAAAGGCCG CTACTGCATG CAGCTGTCCC AGATCCAGGG ACTGATTGGC
AGTGTGGAGG AGCAGCTGGC CCAGCTACGC TGTGAGATGG AGCAGCAGAG CCAGGAGTAC
CAGATCTTGC TGGATGTGAA GACGCGGCTG GAGCAGGAGA TTGCCACCTA CCGCCGCCTG
CTGGAGGGCG AGGATGCCCA CCTTTCCTCC CAGCAAGCAT CTGGCCAATC CTATTCTTCC
CGCGAGGTCT TCACCTCCTC CTCGTCCTCT TCGAGCCGTC AGACCCGGCC CATCCTCAAG
GAGCAGAGCT CATCCAGCTT CAGCCAGGGC CAGAGCTCCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005548.2
CDS140..1561
Misc Feature(1)140..487(+)
Misc Feature(2)485..1420(+)
Misc Feature(3)488..1411(+)
Misc Feature(4)488..595(+)
Misc Feature(5)596..649(+)
Misc Feature(6)650..925(+)
Misc Feature(7)854..1120(+)
Misc Feature(8)926..994(+)
Misc Feature(9)995..1411(+)
Misc Feature(10)1412..1558(+)
Exon (1)1..670
Gene:KRT16
Gene Synonym:
Exon (2)671..753
Gene:KRT16
Gene Synonym:
Exon (3)754..910
Gene:KRT16
Gene Synonym:
Exon (4)911..1072
Gene:KRT16
Gene Synonym:
Exon (5)1073..1198
Gene:KRT16
Gene Synonym:
Exon (6)1199..1419
Gene:KRT16
Gene Synonym:
Exon (7)1420..1466
Gene:KRT16
Gene Synonym:
Exon (8)1467..1718
Gene:KRT16
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_005557
Organism Homo sapiens (human)
Definition Homo sapiens keratin 16, type I (KRT16), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005557

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGACCACCT GCAGCCGCCA GTTCACCTCC TCCAGCTCCA TGAAGGGCTC CTGCGGCATC 
GGAGGCGGCA TCGGGGGCGG CTCCAGCCGC ATCTCCTCCG TCCTGGCCGG AGGGTCCTGC
CGTGCCCCCA GCACCTACGG GGGCGGCCTG TCTGTCTCCT CTCGCTTCTC CTCTGGGGGA
GCCTGCGGGC TGGGGGGCGG CTATGGCGGT GGCTTCAGCA GCAGCAGCAG CTTTGGTAGT
GGCTTCGGGG GAGGATATGG TGGTGGCCTT GGTGCTGGCT TCGGTGGTGG CTTGGGTGCT
GGCTTTGGTG GTGGTTTTGC TGGTGGTGAT GGGCTTCTGG TGGGCAGTGA GAAGGTGACC
ATGCAGAACC TCAATGACCG CCTGGCCTCC TACCTGGACA AGGTGCGTGC TCTGGAGGAG
GCCAACGCCG ACCTGGAAGT GAAGATCCGT GACTGGTACC AGAGGCAGCG GCCCAGTGAG
ATCAAAGACT ACAGTCCCTA CTTCAAGACC ATCGAGGACC TGAGGAACAA GATCATTGCG
GCCACCATTG AGAATGCGCA GCCCATTTTG CAGATTGACA ATGCCAGGCT GGCAGCCGAT
GACTTCAGGA CCAAGTATGA GCATGAACTG GCCCTGCGGC AGACTGTGGA GGCCGACGTC
AATGGCCTGC GCCGGGTGTT GGATGAGCTG ACCCTGGCCA GGACTGACCT GGAGATGCAG
ATCGAAGGCC TGAAGGAGGA GCTGGCCTAC CTGAGGAAGA ACCACGAGGA GGAGATGCTT
GCTCTGAGAG GTCAGACCGG CGGAGATGTG AACGTGGAGA TGGATGCTGC ACCTGGCGTG
GACCTGAGCC GCATCCTGAA TGAGATGCGT GACCAGTACG AGCAGATGGC AGAGAAAAAC
CGCAGAGACG CTGAGACCTG GTTCCTGAGC AAGACCGAGG AGCTGAACAA AGAAGTGGCC
TCCAACAGCG AACTGGTACA GAGCAGCCGC AGTGAGGTGA CGGAGCTCCG GAGGGTGCTC
CAGGGCCTGG AGATTGAGCT GCAGTCCCAG CTCAGCATGA AAGCATCCCT GGAGAACAGC
CTGGAGGAGA CCAAAGGCCG CTACTGCATG CAGCTGTCCC AGATCCAGGG ACTGATTGGC
AGTGTGGAGG AGCAGCTGGC CCAGCTACGC TGTGAGATGG AGCAGCAGAG CCAGGAGTAC
CAGATCTTGC TGGATGTGAA GACGCGGCTG GAGCAGGAGA TTGCCACCTA CCGCCGCCTG
CTGGAGGGCG AGGATGCCCA CCTTTCCTCC CAGCAAGCAT CTGGCCAATC CTATTCTTCC
CGCGAGGTCT TCACCTCCTC CTCGTCCTCT TCGAGCCGTC AGACCCGGCC CATCCTCAAG
GAGCAGAGCT CATCCAGCTT CAGCCAGGGC CAGAGCTCCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A new KRT16 mutation associated with a phenotype of pachyonychia congenita
Exp. Dermatol. 22 (12), 838-839 (2013)
Paris F, Hurtado C, Azon A, Aguado L and Vizmanos JL.


book

Keratin 16 regulates innate immunity in response to epidermal barrier breach
Proc. Natl. Acad. Sci. U.S.A. 110 (48), 19537-19542 (2013)
Lessard JC, Pina-Paz S, Rotty JD, Hickerson RP, Kaspar RL, Balmain A and Coulombe PA.


book

Identification of prognosis-relevant subgroups in patients with chemoresistant triple-negative breast cancer
Clin. Cancer Res. 19 (10), 2723-2733 (2013)
Yu KD, Zhu R, Zhan M, Rodriguez AA, Yang W, Wong S, Makris A, Lehmann BD, Chen X, Mayer I, Pietenpol JA, Shao ZM, Symmans WF and Chang JC.


book

A review of the clinical phenotype of 254 patients with genetically confirmed pachyonychia congenita
J. Am. Acad. Dermatol. 67 (4), 680-686 (2012)
Eliason MJ, Leachman SA, Feng BJ, Schwartz ME and Hansen CD.


book

Two novel de novo mutations of KRT6A and KRT16 genes in two Chinese pachyonychia congenita pedigrees with fissured tongue or diffuse plantar keratoderma
Eur J Dermatol 22 (4), 476-480 (2012)
Du ZF, Xu CM, Zhao Y, Liu WT, Chen XL, Chen CY, Fang H, Ke HP and Zhang XN.


book

cDNA cloning and bacterial expression of the human type I keratin 16
Biochem. Biophys. Res. Commun. 215 (2), 517-523 (1995)
Paladini RD, Takahashi K, Gant TM and Coulombe PA.


book

Pachyonychia Congenita
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Smith,F.J.D., Hansen,C.D., Hull,P.R., Kaspar,R.L., Schwartz,M.E., McLean,W.H.I., Milstone,L.M., van Steensel,M., O'Toole,E. and Sprecher,E.


book

Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes
Electrophoresis 13 (12), 960-969 (1992)
Rasmussen HH, van Damme J, Puype M, Gesser B, Celis JE and Vandekerckhove J.


book

A group of type I keratin genes on human chromosome 17: characterization and expression
Mol. Cell. Biol. 8 (2), 722-736 (1988)
Rosenberg M, RayChaudhury A, Shows TB, Le Beau MM and Fuchs E.


book

Three tightly linked genes encoding human type I keratins: conservation of sequence in the 5'-untranslated leader and 5'-upstream regions of coexpressed keratin genes
Mol. Cell. Biol. 6 (2), 539-548 (1986)
RayChaudhury,A., Marchuk,D., Lindhurst,M. and Fuchs,E.


 
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