×

KRT83 cDNA ORF clone, Homo sapiens (human)

Gene Symbol KRT83
Entrez Gene ID 3889
Full Name keratin 83, type II
Synonyms HB3, Hb-3, KRTHB3
General protein information
Preferred Names
keratin, type II cuticular Hb3
Names
keratin, type II cuticular Hb3
K83
hHb3
keratin-83
hair keratin K2.10
type-II keratin Kb23
keratin, hair, basic, 3
hard keratin, type II, 3
type II hair keratin Hb3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12q13

Summary The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this hair keratin, as well as KRTHB1 and KRTHB6, is found primarily in the hair cortex. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

602765

Disorder Html: Monilethrix, 158000 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_002282 NP_002273 keratin, type II cuticular Hb3


Homo sapiens (human) KRT83 NP_002273.3
Pan troglodytes (chimpanzee) KRT83 XP_003313733.1
Macaca mulatta (Rhesus monkey) KRT83 XP_001093322.1
Bos taurus (cattle) KRT83 NP_001076854.1
Mus musculus (house mouse) 5430421N21Rik NP_001188252.1
Rattus norvegicus (Norway rat) Kb23 NP_001008813.1


The following KRT83 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KRT83 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19697 NM_002282 Homo sapiens keratin 83, type II (KRT83), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19697
Clone ID Related Accession (Same CDS sequence) NM_002282
Accession Version NM_002282.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product keratin, type II cuticular Hb3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Michael Rogers. The reference sequence was derived from AC121757.8 and BC069546.1. This sequence is a reference standard in the RefSeqGene project. On or before Feb 9, 2014 this sequence version replaced gi:578796637, gi:410169881, gi:15431322. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: X99141.1, BC128063.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145313, SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACCTGTG GCTTCAACTC CATAGGCTGT GGGTTCCGCC CTGGAAACTT CAGCTGTGTC 
TCTGCCTGCG GGCCCCGGCC AAGCCGCTGC TGCATCACCG CCGCCCCCTA CCGCGGCATC
TCCTGCTACC GCGGCCTCAC CGGGGGCTTT GGCAGCCACA GCGTGTGCGG GGGCTTCCGC
GCCGGCTCCT GCGGACGCAG CTTCGGCTAC CGCTCCGGGG GCGTGTGCGG ACCCAGCCCC
CCATGCATCA CCACCGTGTC GGTCAACGAG AGCCTCCTCA CGCCCCTCAA CCTGGAGATA
GACCCCAACG CGCAGTGCGT GAAGCAGGAG GAGAAGGAGC AGATCAAGTC CCTCAACAGC
AGATTCGCGG CCTTCATCGA CAAGGTGCGC TTCCTGGAGC AGCAGAACAA GCTGCTGGAG
ACAAAGCTGC AGTTCTACCA AAACCGCGAG TGCTGCCAGA GTAACCTGGA GCCCCTGTTT
GCTGGCTACA TCGAGACTCT GCGGCGGGAG GCCGAGTGCG TGGAGGCTGA CAGTGGGAGG
CTGGCCTCAG AGCTCAACCA CGTGCAGGAG GTGCTGGAGG GCTACAAGAA GAAGTATGAA
GAAGAAGTAG CACTTCGAGC CACAGCAGAG AACGAGTTTG TGGCTCTAAA GAAGGATGTG
GACTGCGCCT ACCTCCGCAA GTCAGACCTG GAGGCCAACG TGGAGGCCCT GATCCAGGAG
ATTGACTTCC TGAGGCGGCT GTACGAGGAG GAGATCCGCA TTCTCCAATC CCACATCTCA
GACACCTCCG TGGTTGTCAA GCTGGACAAC AGCCGGGACC TGAACATGGA CTGCATCGTT
GCCGAGATCA AGGCACAGTA TGATGACATT GCCACCCGTA GCCGGGCTGA GGCCGAGTCC
TGGTATCGCA GCAAGTGTGA GGAGATGAAG GCCACAGTGA TCAGGCACGG GGAGACCCTG
CGCCGCACCA AGGAGGAGAT CAACGAGCTG AACCGCATGA TCCAGAGGCT GACAGCCGAG
GTGGAGAATG CCAAGTGCCA GAACTCCAAG CTGGAAGCTG CGGTGGCCCA GTCTGAGCAG
CAGGGTGAGG CGGCCCTCAG TGATGCCCGC TGCAAGCTGG CCGAGCTGGA GGGCGCCCTG
CAGAAGGCCA AGCAAGACAT GGCCTGCCTG ATCAGGGAGT ACCAGGAGGT GATGAACTCC
AAGCTAGGCC TGGATATCGA GATCGCCACC TACAGGCGCC TGCTGGAGGG CGAGGAGCAG
AGGCTGTGTG AAGGTGTTGA AGCTGTGAAT GTCTGTGTCA GCAGCTCCCG GGGTGGGGTT
GTGTGCGGGG ATCTCTGCGT GTCGGGCTCC CGGCCGGTGA CGGGCAGCGT CTGCAGTGCC
CCCTGCAACG GGAACCTGGT GGTGAGCACT GGTTTGTGCA AGCCCTGTGG CCAGCTGAAC
ACCACCTGTG GAGGGGGCTC CTGCGGCCAG GGGAGGCATT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002273.3
CDS64..1545
Misc Feature(1)64..396(+)
Misc Feature(2)391..1326(+)
Misc Feature(3)397..1317(+)
Misc Feature(4)397..501(+)
Misc Feature(5)502..531(+)
Misc Feature(6)532..834(+)
Misc Feature(7)766..1338(+)
Misc Feature(8)769..1335(+)
Misc Feature(9)835..885(+)
Misc Feature(10)886..1317(+)
Misc Feature(11)1318..1542(+)
Exon (1)1..447
Gene:KRT83
Gene Synonym:
Exon (2)448..656
Gene:KRT83
Gene Synonym:
Exon (3)657..717
Gene:KRT83
Gene Synonym:
Exon (4)718..813
Gene:KRT83
Gene Synonym:
Exon (5)814..978
Gene:KRT83
Gene Synonym:
Exon (6)979..1104
Gene:KRT83
Gene Synonym:
Exon (7)1105..1325
Gene:KRT83
Gene Synonym:
Exon (8)1326..1357
Gene:KRT83
Gene Synonym:
Exon (9)1358..1875
Gene:KRT83
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002282
Organism Homo sapiens (human)
Definition Homo sapiens keratin 83, type II (KRT83), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002282

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACCTGTG GCTTCAACTC CATAGGCTGT GGGTTCCGCC CTGGAAACTT CAGCTGTGTC 
TCTGCCTGCG GGCCCCGGCC AAGCCGCTGC TGCATCACCG CCGCCCCCTA CCGCGGCATC
TCCTGCTACC GCGGCCTCAC CGGGGGCTTT GGCAGCCACA GCGTGTGCGG GGGCTTCCGC
GCCGGCTCCT GCGGACGCAG CTTCGGCTAC CGCTCCGGGG GCGTGTGCGG ACCCAGCCCC
CCATGCATCA CCACCGTGTC GGTCAACGAG AGCCTCCTCA CGCCCCTCAA CCTGGAGATA
GACCCCAACG CGCAGTGCGT GAAGCAGGAG GAGAAGGAGC AGATCAAGTC CCTCAACAGC
AGATTCGCGG CCTTCATCGA CAAGGTGCGC TTCCTGGAGC AGCAGAACAA GCTGCTGGAG
ACAAAGCTGC AGTTCTACCA AAACCGCGAG TGCTGCCAGA GTAACCTGGA GCCCCTGTTT
GCTGGCTACA TCGAGACTCT GCGGCGGGAG GCCGAGTGCG TGGAGGCTGA CAGTGGGAGG
CTGGCCTCAG AGCTCAACCA CGTGCAGGAG GTGCTGGAGG GCTACAAGAA GAAGTATGAA
GAAGAAGTAG CACTTCGAGC CACAGCAGAG AACGAGTTTG TGGCTCTAAA GAAGGATGTG
GACTGCGCCT ACCTCCGCAA GTCAGACCTG GAGGCCAACG TGGAGGCCCT GATCCAGGAG
ATTGACTTCC TGAGGCGGCT GTACGAGGAG GAGATCCGCA TTCTCCAATC CCACATCTCA
GACACCTCCG TGGTTGTCAA GCTGGACAAC AGCCGGGACC TGAACATGGA CTGCATCGTT
GCCGAGATCA AGGCACAGTA TGATGACATT GCCACCCGTA GCCGGGCTGA GGCCGAGTCC
TGGTATCGCA GCAAGTGTGA GGAGATGAAG GCCACAGTGA TCAGGCACGG GGAGACCCTG
CGCCGCACCA AGGAGGAGAT CAACGAGCTG AACCGCATGA TCCAGAGGCT GACAGCCGAG
GTGGAGAATG CCAAGTGCCA GAACTCCAAG CTGGAAGCTG CGGTGGCCCA GTCTGAGCAG
CAGGGTGAGG CGGCCCTCAG TGATGCCCGC TGCAAGCTGG CCGAGCTGGA GGGCGCCCTG
CAGAAGGCCA AGCAAGACAT GGCCTGCCTG ATCAGGGAGT ACCAGGAGGT GATGAACTCC
AAGCTAGGCC TGGATATCGA GATCGCCACC TACAGGCGCC TGCTGGAGGG CGAGGAGCAG
AGGCTGTGTG AAGGTGTTGA AGCTGTGAAT GTCTGTGTCA GCAGCTCCCG GGGTGGGGTT
GTGTGCGGGG ATCTCTGCGT GTCGGGCTCC CGGCCGGTGA CGGGCAGCGT CTGCAGTGCC
CCCTGCAACG GGAACCTGGT GGTGAGCACT GGTTTGTGCA AGCCCTGTGG CCAGCTGAAC
ACCACCTGTG GAGGGGGCTC CTGCGGCCAG GGGAGGCATT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Shotgun proteomics reveals specific modulated protein patterns in tears of patients with primary open angle glaucoma naive to therapy
Mol Biosyst 9 (6), 1108-1116 (2013)
Pieragostino D, Agnifili L, Fasanella V, D'Aguanno S, Mastropasqua R, Di Ilio C, Sacchetta P, Urbani A and Del Boccio P.


book

Hair greying is associated with active hair growth
Br. J. Dermatol. 165 (6), 1183-1189 (2011)
Choi HI, Choi GI, Kim EK, Choi YJ, Sohn KC, Lee Y, Kim CD, Yoon TJ, Sohn HJ, Han SH, Kim S, Lee JH and Lee YH.


book

New consensus nomenclature for mammalian keratins
J. Cell Biol. 174 (2), 169-174 (2006)
Schweizer J, Bowden PE, Coulombe PA, Langbein L, Lane EB, Magin TM, Maltais L, Omary MB, Parry DA, Rogers MA and Wright MW.


book

A missense mutation in the type II hair keratin hHb3 is associated with monilethrix
J. Med. Genet. 42 (3), E19 (2005)
van Steensel MA, Steijlen PM, Bladergroen RS, Vermeer M and van Geel M.


book

Keratins of the human hair follicle
Int. Rev. Cytol. 243, 1-78 (2005)
Langbein L and Schweizer J.


book

The catalog of human hair keratins. II. Expression of the six type II members in the hair follicle and the combined catalog of human type I and II keratins
J. Biol. Chem. 276 (37), 35123-35132 (2001)
Langbein L, Rogers MA, Winter H, Praetzel S and Schweizer J.


book

Characterization of a 300 kbp region of human DNA containing the type II hair keratin gene domain
J. Invest. Dermatol. 114 (3), 464-472 (2000)
Rogers MA, Winter H, Langbein L, Wolf C and Schweizer J.


book

Sequences and differential expression of three novel human type-II hair keratins
Differentiation 61 (3), 187-194 (1997)
Rogers MA, Langbein L, Praetzel S, Moll I, Krieg T, Winter H and Schweizer J.


 
*
*
*
*