Email to GenScript

KRT86 keratin 86, type II [Homo sapiens (human)]

Gene Symbol KRT86
Entrez Gene ID 3892
Full Name keratin 86, type II
Synonyms HB6, Hb1, KRTHB1, KRTHB6, MNX, hHb6
General protein information
Preferred Names
keratin, type II cuticular Hb6
Names
keratin, type II cuticular Hb6
K86
keratin-86
hair keratin K2.11
keratin protein HB6
type-II keratin Kb26
hard keratin, type II, 6
type II hair keratin Hb6
keratin, hair, basic, 6 (monilethrix)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12q13

Summary The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this hair keratin, as well as KRTHB1 and KRTHB3, is found primarily in the hair cortex. Mutations in this gene and KRTHB1 have been observed in patients with a rare dominant hair disease, monilethrix. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

601928

Disorder Html: Monilethrix, 158000 (3)





The following KRT86 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KRT86 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu46550 XM_005268866 PREDICTED: Homo sapiens keratin 86, type II (KRT86), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu17915 XM_011538336 PREDICTED: Homo sapiens keratin 86, type II (KRT86), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu17915 XM_011538337 PREDICTED: Homo sapiens keratin 86, type II (KRT86), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu17915 XM_011538338 PREDICTED: Homo sapiens keratin 86, type II (KRT86), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu17915 NM_002284 Homo sapiens keratin 86, type II (KRT86), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu46550
Accession Version XM_005268866.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1692bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product keratin, type II cuticular Hb6 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578823713. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGTGTGAGG AATTAAAGGC AACTGTGCAG AAACACACGC AGAGCCTGAA GCCCAGCAAG 
GAGGATCTGA ACAGGCTTAA TCAGGCCATC CAGTGGCTGA CGGTGGAGGT GGGCAGTGCT
GAGAGTCAGG CCTACAGAGG TGCAAGTAGT GAACGCCTGA CGCCCCGACC ACTGTGCTCT
CCATTCGGAC GTCTCCATCC TCAGAACCTC CTCTCTTCCC CAAAAAGCAC CATGACTTGT
GGATCTTACT GTGGTGGCCG CGCCTTCAGC TGCATCTCGG CCTGCGGGCC CCGGCCCGGC
CGCTGCTGCA TCACCGCCGC CCCCTACCGT GGCATCTCCT GCTACCGCGG CCTCACCGGG
GGCTTCGGCA GCCACAGCGT GTGCGGAGGC TTTCGGGCCG GCTCCTGCGG ACGCAGCTTC
GGCTACCGCT CCGGGGGCGT GTGCGGGCCC AGTCCCCCAT GCATCACCAC CGTGTCGGTC
AACGAGAGCC TCCTCACGCC CCTCAACCTG GAGATCGACC CCAACGCGCA GTGCGTGAAG
CAGGAGGAGA AGGAGCAGAT CAAGTCCCTC AACAGCAGGT TCGCGGCCTT CATCGACAAG
GTGCGCTTCC TGGAGCAGCA GAACAAACTG CTGGAGACAA AGCTGCAGTT CTACCAGAAC
CGCGAGTGTT GCCAGAGCAA CCTGGAGCCC CTGTTTGAGG GCTACATCGA GACTCTGCGG
CGGGAGGCCG AGTGCGTGGA GGCCGACAGC GGGAGGCTGG CCTCAGAGCT TAACCACGTG
CAGGAGGTGC TGGAGGGCTA CAAGAAGAAG TATGAGGAGG AGGTTTCTCT GAGAGCAACA
GCTGAGAACG AGTTTGTGGC TCTGAAGAAG GATGTGGACT GCGCCTACCT CCGCAAATCA
GACCTGGAGG CCAATGTGGA GGCCCTGATC CAGGAGATCG ACTTCCTGAG GCGGCTGTAT
GAGGAGGAGA TCCGCGTTCT CCAGTCCCAC ATCTCAGACA CCTCCGTGGT TGTCAAGCTG
GACAACAGCC GGGACCTGAA CATGGACTGC ATCATTGCCG AGATCAAGGC ACAGTACGAT
GACATTGTCA CCCGTAGCCG GGCTGAGGCC GAGTCCTGGT ACCGCAGCAA GTGTGAGGAG
ATGAAGGCCA CGGTGATCAG GCACGGGGAG ACCCTGCGCC GCACCAAGGA GGAGATCAAC
GAGCTGAACC GCATGATCCA GAGGCTGACG GCTGAGGTGG AGAATGCCAA GTGCCAGAAT
TCCAAGCTGG AGGCTGCGGT GGCTCAGTCT GAGCAGCAGG GTGAGGCGGC CCTCAGCGAT
GCCCGCTGCA AGTTGGCCGA GCTGGAGGGT GCCCTGCAGA AGGCCAAGCA GGACATGGCC
TGCCTGATCA GGGAGTACCA GGAGGTGATG AACTCCAAGC TGGGCCTGGA CATCGAGATC
GCCACCTACA GGCGCCTGCT GGAGGGCGAG GAGCAGAGGC TGTGCGAGGG CGTCGGCTCG
GTGAATGTCT GCGTCAGCAG CTCCCGCGGT GGCGTTGTCT GTGGCGATCT CTGCGCCTCC
ACTACTGCCC CTGTTGTCTC CACCAGAGTC AGTAGCGTCC CCAGCAACAG CAACGTGGTG
GTGGGCACTA CTAACGCCTG CGCCCCCTCC GCCCGGGTTG GCGTCTGCGG CGGCAGCTGT
AAGAGGTGCT AG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005268923.1
CDS90..1781
Misc Feature(1)633..1568(+)
Misc Feature(2)993..1340(+)
Translation
Position Chain Variation Link
7 7 -, g dbSNP:760487556
18 18 c, t dbSNP:117796387
36 36 a, g dbSNP:77386533
40 40 c, t dbSNP:752367873
113 113 c, t dbSNP:140173379
183 183 c, t dbSNP:558242010
191 191 ca, gg dbSNP:35306692
191 191 c, g dbSNP:11834151
192 192 a, g dbSNP:11834152
212 212 a, g dbSNP:145174534
236 236 c, t dbSNP:141027796
245 245 c, t dbSNP:557672714
250 250 c, t dbSNP:1894033
253 253 c, g dbSNP:557287445
275 275 a, c, t dbSNP:756381120
278 278 a, c dbSNP:749346976
280 280 a, g dbSNP:755444963
288 288 a, c, t dbSNP:56016927
289 289 c, t dbSNP:559412483
297 297 c, g dbSNP:773532478
300 300 c, g dbSNP:746715296
307 307 a, c dbSNP:770571275
311 311 a, c dbSNP:776039120
312 312 a, g dbSNP:528526293
316 316 a, g dbSNP:367636606
320 320 c, g dbSNP:775628307
324 324 a, t dbSNP:762980858
329 329 g, t dbSNP:764023107
330 330 a, g dbSNP:751348438
332 332 a, c dbSNP:756434438
334 334 a, c dbSNP:766555284
336 336 c, t dbSNP:117662922
341 341 g, t dbSNP:755139437
347 347 a, c, g, t dbSNP:201329364
348 348 a, c dbSNP:758877634
350 350 c, t dbSNP:368082897
352 352 a, c dbSNP:747337849
353 353 c, t dbSNP:371864554
357 357 a, g dbSNP:780844187
358 358 c, g dbSNP:565104845
363 363 a, g, t dbSNP:530949768
368 368 c, g dbSNP:199538989
372 372 g, t dbSNP:550585959
374 374 c, t dbSNP:74651927
375 375 a, g dbSNP:774291422
380 380 c, g, t dbSNP:201694571
382 382 a, c, g dbSNP:754153541
384 384 -, c dbSNP:745354445
386 386 c, g dbSNP:765348086
387 387 a, g, t dbSNP:752727601
390 390 a, c dbSNP:778190510
397 397 a, g dbSNP:751938228
398 398 c, t dbSNP:201420491
404 404 c, t dbSNP:781406879
406 406 c, t dbSNP:566680440
407 407 a, c, t dbSNP:112261068
412 412 c, g dbSNP:748739670
413 413 c, g dbSNP:768727542
417 417 c, t dbSNP:774487692
418 418 a, c, g, t dbSNP:552266351
419 419 c, t dbSNP:772858714
422 422 c, t dbSNP:759824354
425 425 c, g dbSNP:765405708
427 427 a, c dbSNP:202141424
428 428 c, t dbSNP:762965684
435 435 c, t dbSNP:764149570
436 436 a, g dbSNP:752176981
437 437 c, t dbSNP:757675096
443 443 a, c, t dbSNP:781658784
446 446 c, t dbSNP:755794129
447 447 c, g dbSNP:779794188
448 448 a, g dbSNP:748935718
449 449 c, g dbSNP:754598294
455 455 c, t dbSNP:778531514
457 457 c, g dbSNP:748162043
464 464 c, t dbSNP:771852441
468 468 a, g, t dbSNP:773093741
474 474 c, g dbSNP:376835872
475 475 a, g dbSNP:557368421
481 481 c, t dbSNP:574036785
483 483 c, t dbSNP:764200311
484 484 a, g dbSNP:774273613
488 488 c, t dbSNP:762407305
489 489 c, g, t dbSNP:369728157
492 492 g, t dbSNP:756604025
496 496 -, ctt dbSNP:775534639
497 497 c, t dbSNP:766845192
499 499 -, gacgca dbSNP:760642690
501 501 c, g dbSNP:753581743
509 509 c, t dbSNP:754651481
510 510 c, g dbSNP:778504570
512 512 a, c dbSNP:747568260
515 515 -, c dbSNP:768654938
516 516 a, c dbSNP:758364190
517 517 a, g dbSNP:57242951
521 521 c, t dbSNP:553105016
522 522 a, c, g dbSNP:573121340
524 524 c, g dbSNP:749564887
525 525 a, g dbSNP:768820064
542 542 c, t dbSNP:774523262
543 543 a, c dbSNP:761738178
544 544 c, t dbSNP:767618159
546 546 c, g dbSNP:773655908
552 552 a, g dbSNP:761233503
560 560 a, c dbSNP:766898287
566 566 a, g dbSNP:754205786
586 586 c, t dbSNP:754706890
595 595 a, g dbSNP:764732778
608 608 c, t dbSNP:545137193
614 614 c, t dbSNP:757904259
616 616 c, t dbSNP:777333199
617 617 a, g, t dbSNP:199751260
621 621 g, t dbSNP:780990129
624 624 a, g dbSNP:745624392
625 625 c, t dbSNP:768877244
654 654 -, ccctcaac dbSNP:776225547
660 660 a, c, g dbSNP:61091894
668 668 a, g dbSNP:58580222
672 672 a, g dbSNP:748394653
673 673 a, c dbSNP:60612575
717 717 a, c dbSNP:537434322
735 735 a, c dbSNP:771522388
736 736 a, c dbSNP:58717266
811 811 a, g dbSNP:548127873
825 825 g, t dbSNP:567721642
956 956 a, g dbSNP:79398941
961 961 a, g dbSNP:56677856
963 963 a, g dbSNP:781043236
969 969 a, t dbSNP:779212628
971 971 c, t dbSNP:377005978
981 981 c, t dbSNP:199678774
982 982 a, g, t dbSNP:531934335
985 985 a, t dbSNP:772288102
986 986 a, g dbSNP:201521867
993 993 a, c dbSNP:747016737
997 997 a, t dbSNP:771581226
999 999 a, g dbSNP:777336849
1002 1002 a, g dbSNP:760117096
1004 1004 c, t dbSNP:200750977
1005 1005 a, g dbSNP:775905498
1012 1012 a, c, t dbSNP:762539212
1018 1018 a, t dbSNP:751075241
1024 1024 a, g dbSNP:761368875
1025 1025 g, t dbSNP:200085173
1028 1028 c, t dbSNP:778154101
1029 1029 a, g dbSNP:756052236
1039 1039 a, g dbSNP:201433700
1041 1041 c, t dbSNP:111429470
1042 1042 a, g dbSNP:753627634
1043 1043 a, g dbSNP:758552461
1046 1046 a, g dbSNP:778081882
1047 1047 c, t dbSNP:759397233
1048 1048 a, c, g, t dbSNP:202150817
1052 1052 a, c, g dbSNP:78831098
1053 1053 a, g dbSNP:775752662
1060 1060 g, t dbSNP:776699267
1062 1062 c, t dbSNP:368604832
1063 1063 a, g, t dbSNP:765173749
1064 1064 c, t dbSNP:58523335
1065 1065 a, g dbSNP:61914259
1072 1072 a, g dbSNP:750485674
1073 1073 a, g dbSNP:756277678
1075 1075 c, t dbSNP:779962943
1076 1076 c, g, t dbSNP:61915660
1084 1084 c, t dbSNP:779276684
1085 1085 a, g dbSNP:748444485
1086 1086 g, t dbSNP:367712000
1088 1088 c, t dbSNP:371492790
1091 1091 c, g dbSNP:112956699
1094 1094 c, g dbSNP:770517793
1095 1095 a, g dbSNP:369679589
1098 1098 a, g, t dbSNP:759569087
1119 1119 c, t dbSNP:527924718
1120 1120 a, g dbSNP:371922719
1132 1132 c, g, t dbSNP:763786271
1140 1140 a, g dbSNP:760783190
1143 1143 a, g dbSNP:756033789
1148 1148 c, t dbSNP:200472524
1149 1149 a, g dbSNP:376581340
1153 1153 g, t dbSNP:755479412
1154 1154 c, t dbSNP:779333951
1161 1161 a, c dbSNP:753187461
1162 1162 a, c, t dbSNP:369890550
1166 1166 c, t dbSNP:777969989
1167 1167 a, g dbSNP:377327853
1173 1173 a, t dbSNP:770441867
1176 1176 a, g, t dbSNP:780785303
1182 1182 c, g, t dbSNP:199724105
1183 1183 a, g dbSNP:376573652
1184 1184 c, t dbSNP:768542333
1188 1188 c, t dbSNP:762794979
1189 1189 a, g dbSNP:370858159
1192 1192 c, g dbSNP:766441670
1193 1193 c, t dbSNP:190756867
1194 1194 a, g dbSNP:759668482
1197 1197 a, c, g dbSNP:765295167
1198 1198 a, c dbSNP:376319970
1199 1199 c, t dbSNP:569717946
1200 1200 a, g, t dbSNP:183358379
1204 1204 c, g dbSNP:780655613
1211 1211 c, t dbSNP:374553856
1212 1212 c, t dbSNP:201943606
1213 1213 a, g dbSNP:199840628
1214 1214 c, g dbSNP:534563196
1231 1231 c, t dbSNP:201760569
1236 1236 a, g dbSNP:762354129
1240 1240 c, t dbSNP:372547107
1241 1241 a, g dbSNP:750775368
1247 1247 c, g dbSNP:760403544
1253 1253 c, g, t dbSNP:144028073
1254 1254 a, g dbSNP:754512829
1262 1262 c, t dbSNP:778453366
1263 1263 c, t dbSNP:752624557
1264 1264 a, t dbSNP:758283340
1266 1266 a, c, t dbSNP:369280939
1267 1267 a, g dbSNP:770017878
1269 1269 c, t dbSNP:780315495
1270 1270 a, g dbSNP:557621183
1283 1283 a, g dbSNP:768661795
1285 1285 a, t dbSNP:774470730
1288 1288 a, g dbSNP:373358594
1289 1289 c, t dbSNP:772636597
1291 1291 a, g dbSNP:773722982
1298 1298 c, t dbSNP:376127132
1299 1299 c, t dbSNP:139895699
1300 1300 a, g dbSNP:753470822
1302 1302 a, c, g dbSNP:759203655
1304 1304 a, g dbSNP:752211932
1305 1305 a, t dbSNP:758338480
1307 1307 a, c dbSNP:777631631
1313 1313 a, g dbSNP:560252337
1319 1319 a, g dbSNP:374471358
1320 1320 c, g dbSNP:781014025
1322 1322 c, g, t dbSNP:2032360
1323 1323 a, g dbSNP:376386930
1328 1328 a, g dbSNP:202026667
1331 1331 a, g dbSNP:772262266
1335 1335 c, g dbSNP:773921393
1343 1343 c, t dbSNP:747486507
1344 1344 a, c dbSNP:771471186
1349 1349 c, t dbSNP:763647926
1353 1353 a, t dbSNP:368204968
1354 1354 a, g, t dbSNP:372469887
1356 1356 c, t dbSNP:191545221
1359 1359 a, g dbSNP:756000963
1364 1364 c, t dbSNP:4761788
1366 1366 c, t dbSNP:201286632
1367 1367 a, g dbSNP:369312934
1370 1370 a, g dbSNP:778028956
1371 1371 a, g dbSNP:747018877
1373 1373 a, c, t dbSNP:28733260
1382 1382 g, t dbSNP:746358096
1386 1386 c, g, t dbSNP:770237949
1389 1389 c, g dbSNP:201484793
1396 1396 c, t dbSNP:534973333
1397 1397 a, g dbSNP:773861385
1399 1399 c, t dbSNP:202169108
1401 1401 c, g dbSNP:376312545
1406 1406 c, t dbSNP:11170086
1407 1407 a, g dbSNP:760506150
1411 1411 a, c dbSNP:766217821
1413 1413 c, t dbSNP:201833908
1414 1414 a, g dbSNP:373932045
1416 1416 c, t dbSNP:578093160
1422 1422 c, t dbSNP:11170087
1423 1423 c, t dbSNP:757425820
1427 1427 a, c, g, t dbSNP:376051219
1428 1428 a, g, t dbSNP:200353690
1431 1431 c, t dbSNP:749593452
1438 1438 g, t dbSNP:768991511
1439 1439 c, t dbSNP:11170088
1444 1444 c, t dbSNP:747720953
1450 1450 a, t dbSNP:771660500
1451 1451 g, t dbSNP:772546220
1465 1465 g, t dbSNP:760697549
1476 1476 a, c dbSNP:563550345
1479 1479 a, t dbSNP:776580728
1480 1480 a, g dbSNP:573751348
1484 1484 a, c, g dbSNP:375789021
1489 1489 a, g dbSNP:761921829
1498 1498 a, c, t dbSNP:767661038
1502 1502 c, t dbSNP:756653876
1507 1507 a, g dbSNP:542735645
1516 1516 c, t dbSNP:754335203
1523 1523 c, t dbSNP:755372953
1524 1524 a, c, g dbSNP:60687604
1529 1529 c, t dbSNP:747856782
1530 1530 a, g dbSNP:757946783
1532 1532 c, t dbSNP:777341221
1542 1542 c, t dbSNP:749369523
1543 1543 a, g dbSNP:199951808
1544 1544 c, t dbSNP:368480216
1548 1548 c, t dbSNP:370091437
1553 1553 a, g dbSNP:769763274
1555 1555 a, g dbSNP:775487582
1556 1556 a, c, t dbSNP:372174710
1557 1557 a, g dbSNP:121909129
1559 1559 g, t dbSNP:121909130
1580 1580 a, c dbSNP:749890373
1581 1581 a, g dbSNP:756138046
1584 1584 a, g dbSNP:779918036
1588 1588 a, c dbSNP:562353539
1589 1589 c, g dbSNP:768381688
1595 1595 c, t dbSNP:778718877
1599 1599 a, t dbSNP:747260525
1601 1601 a, c dbSNP:561311116
1602 1602 g, t dbSNP:763403025
1617 1617 a, g dbSNP:769143368
1622 1622 a, c, g dbSNP:527319461
1630 1630 c, g dbSNP:767957618
1642 1642 c, g dbSNP:773044010
1644 1644 a, g dbSNP:760323015
1645 1645 c, t dbSNP:765834889
1655 1655 a, t dbSNP:753350605
1661 1661 c, t dbSNP:754896113
1662 1662 a, c, g dbSNP:200098470
1672 1672 c, g dbSNP:758256483
1673 1673 c, t dbSNP:370419090
1674 1674 a, g dbSNP:746090200
1682 1682 c, t dbSNP:756291633
1686 1686 a, g dbSNP:780026281
1687 1687 a, t dbSNP:749337520
1690 1690 c, t dbSNP:768592222
1693 1693 a, g dbSNP:775033485
1694 1694 c, t dbSNP:748655954
1698 1698 a, g dbSNP:772581641
1706 1706 a, g dbSNP:773647506
1709 1709 c, g dbSNP:374122056
1713 1713 c, g dbSNP:560668616
1725 1725 a, g dbSNP:776126777
1728 1728 c, t dbSNP:532729838
1732 1732 c, t dbSNP:368279953
1735 1735 c, g dbSNP:752688759
1736 1736 a, c dbSNP:758272986
1738 1738 c, t dbSNP:763879273
1740 1740 g, t dbSNP:751326981
1744 1744 c, g, t dbSNP:756310592
1745 1745 g, t dbSNP:749435372
1747 1747 -, t dbSNP:752996118
1751 1751 a, c dbSNP:755171534
1752 1752 c, g, t dbSNP:779081505
1753 1753 c, t dbSNP:772669443
1758 1758 a, g dbSNP:773737109
1761 1761 a, c, g dbSNP:552762476
1768 1768 -, t dbSNP:756555632
1774 1774 a, g dbSNP:771169755
1777 1777 a, g dbSNP:201740931
1778 1778 c, t dbSNP:759097243
1779 1779 c, t dbSNP:769306504
1781 1781 a, g dbSNP:774913128
1784 1784 a, g dbSNP:148309398
1787 1787 g, t dbSNP:763968892
1791 1791 c, g dbSNP:751427480
1793 1793 a, c dbSNP:761704739
1797 1797 g, t dbSNP:548735427
1802 1802 a, c dbSNP:375430561
1803 1803 g, t dbSNP:755188054
1810 1810 a, g dbSNP:779167469
1812 1812 c, g dbSNP:752778941
1813 1813 c, g dbSNP:758531707
1823 1823 a, c dbSNP:568421074
1827 1827 a, c dbSNP:201257801
1840 1840 c, g dbSNP:771115534
1843 1843 c, t dbSNP:554027353
1875 1875 a, g dbSNP:2280949
1887 1887 c, g dbSNP:573713057
1905 1905 c, g dbSNP:559456695
1908 1908 c, t dbSNP:4076515
1942 1942 a, g dbSNP:768815810
1951 1951 -, g dbSNP:775440318
1990 1990 a, g dbSNP:185290279
2001 2001 a, g dbSNP:188381439
2008 2008 a, g dbSNP:548333894
2045 2045 a, g dbSNP:1137013
2052 2052 -, t, tt dbSNP:536030727
2053 2053 -, tt dbSNP:111847567
2060 2060 -, t, tt, ttt dbSNP:369883463
2061 2061 -, t dbSNP:3215437
2068 2068 a, g dbSNP:1137014
2093 2093 c, t dbSNP:1137015
2139 2139 a, g dbSNP:116249711
2198 2198 a, g dbSNP:532647627
2203 2203 c, t dbSNP:546535987
2223 2223 c, g dbSNP:563059388
2241 2241 a, g dbSNP:750820066
2251 2251 c, g dbSNP:141418442
2298 2298 a, c dbSNP:548699342
2314 2314 c, t dbSNP:767008402
2318 2318 a, g dbSNP:369975927
2353 2353 a, g dbSNP:527798405

Target ORF information:

RefSeq Version XM_005268866
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens keratin 86, type II (KRT86), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005268866

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGTGTGAGG AATTAAAGGC AACTGTGCAG AAACACACGC AGAGCCTGAA GCCCAGCAAG 
GAGGATCTGA ACAGGCTTAA TCAGGCCATC CAGTGGCTGA CGGTGGAGGT GGGCAGTGCT
GAGAGTCAGG CCTACAGAGG TGCAAGTAGT GAACGCCTGA CGCCCCGACC ACTGTGCTCT
CCATTCGGAC GTCTCCATCC TCAGAACCTC CTCTCTTCCC CAAAAAGCAC CATGACTTGT
GGATCTTACT GTGGTGGCCG CGCCTTCAGC TGCATCTCGG CCTGCGGGCC CCGGCCCGGC
CGCTGCTGCA TCACCGCCGC CCCCTACCGT GGCATCTCCT GCTACCGCGG CCTCACCGGG
GGCTTCGGCA GCCACAGCGT GTGCGGAGGC TTTCGGGCCG GCTCCTGCGG ACGCAGCTTC
GGCTACCGCT CCGGGGGCGT GTGCGGGCCC AGTCCCCCAT GCATCACCAC CGTGTCGGTC
AACGAGAGCC TCCTCACGCC CCTCAACCTG GAGATCGACC CCAACGCGCA GTGCGTGAAG
CAGGAGGAGA AGGAGCAGAT CAAGTCCCTC AACAGCAGGT TCGCGGCCTT CATCGACAAG
GTGCGCTTCC TGGAGCAGCA GAACAAACTG CTGGAGACAA AGCTGCAGTT CTACCAGAAC
CGCGAGTGTT GCCAGAGCAA CCTGGAGCCC CTGTTTGAGG GCTACATCGA GACTCTGCGG
CGGGAGGCCG AGTGCGTGGA GGCCGACAGC GGGAGGCTGG CCTCAGAGCT TAACCACGTG
CAGGAGGTGC TGGAGGGCTA CAAGAAGAAG TATGAGGAGG AGGTTTCTCT GAGAGCAACA
GCTGAGAACG AGTTTGTGGC TCTGAAGAAG GATGTGGACT GCGCCTACCT CCGCAAATCA
GACCTGGAGG CCAATGTGGA GGCCCTGATC CAGGAGATCG ACTTCCTGAG GCGGCTGTAT
GAGGAGGAGA TCCGCGTTCT CCAGTCCCAC ATCTCAGACA CCTCCGTGGT TGTCAAGCTG
GACAACAGCC GGGACCTGAA CATGGACTGC ATCATTGCCG AGATCAAGGC ACAGTACGAT
GACATTGTCA CCCGTAGCCG GGCTGAGGCC GAGTCCTGGT ACCGCAGCAA GTGTGAGGAG
ATGAAGGCCA CGGTGATCAG GCACGGGGAG ACCCTGCGCC GCACCAAGGA GGAGATCAAC
GAGCTGAACC GCATGATCCA GAGGCTGACG GCTGAGGTGG AGAATGCCAA GTGCCAGAAT
TCCAAGCTGG AGGCTGCGGT GGCTCAGTCT GAGCAGCAGG GTGAGGCGGC CCTCAGCGAT
GCCCGCTGCA AGTTGGCCGA GCTGGAGGGT GCCCTGCAGA AGGCCAAGCA GGACATGGCC
TGCCTGATCA GGGAGTACCA GGAGGTGATG AACTCCAAGC TGGGCCTGGA CATCGAGATC
GCCACCTACA GGCGCCTGCT GGAGGGCGAG GAGCAGAGGC TGTGCGAGGG CGTCGGCTCG
GTGAATGTCT GCGTCAGCAG CTCCCGCGGT GGCGTTGTCT GTGGCGATCT CTGCGCCTCC
ACTACTGCCC CTGTTGTCTC CACCAGAGTC AGTAGCGTCC CCAGCAACAG CAACGTGGTG
GTGGGCACTA CTAACGCCTG CGCCCCCTCC GCCCGGGTTG GCGTCTGCGG CGGCAGCTGT
AAGAGGTGCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17915
Accession Version XM_011538336.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1461bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product keratin, type II cuticular Hb6 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACTTGTG GATCTTACTG TGGTGGCCGC GCCTTCAGCT GCATCTCGGC CTGCGGGCCC 
CGGCCCGGCC GCTGCTGCAT CACCGCCGCC CCCTACCGTG GCATCTCCTG CTACCGCGGC
CTCACCGGGG GCTTCGGCAG CCACAGCGTG TGCGGAGGCT TTCGGGCCGG CTCCTGCGGA
CGCAGCTTCG GCTACCGCTC CGGGGGCGTG TGCGGGCCCA GTCCCCCATG CATCACCACC
GTGTCGGTCA ACGAGAGCCT CCTCACGCCC CTCAACCTGG AGATCGACCC CAACGCGCAG
TGCGTGAAGC AGGAGGAGAA GGAGCAGATC AAGTCCCTCA ACAGCAGGTT CGCGGCCTTC
ATCGACAAGG TGCGCTTCCT GGAGCAGCAG AACAAACTGC TGGAGACAAA GCTGCAGTTC
TACCAGAACC GCGAGTGTTG CCAGAGCAAC CTGGAGCCCC TGTTTGAGGG CTACATCGAG
ACTCTGCGGC GGGAGGCCGA GTGCGTGGAG GCCGACAGCG GGAGGCTGGC CTCAGAGCTT
AACCACGTGC AGGAGGTGCT GGAGGGCTAC AAGAAGAAGT ATGAGGAGGA GGTTTCTCTG
AGAGCAACAG CTGAGAACGA GTTTGTGGCT CTGAAGAAGG ATGTGGACTG CGCCTACCTC
CGCAAATCAG ACCTGGAGGC CAATGTGGAG GCCCTGATCC AGGAGATCGA CTTCCTGAGG
CGGCTGTATG AGGAGGAGAT CCGCGTTCTC CAGTCCCACA TCTCAGACAC CTCCGTGGTT
GTCAAGCTGG ACAACAGCCG GGACCTGAAC ATGGACTGCA TCATTGCCGA GATCAAGGCA
CAGTACGATG ACATTGTCAC CCGTAGCCGG GCTGAGGCCG AGTCCTGGTA CCGCAGCAAG
TGTGAGGAGA TGAAGGCCAC GGTGATCAGG CACGGGGAGA CCCTGCGCCG CACCAAGGAG
GAGATCAACG AGCTGAACCG CATGATCCAG AGGCTGACGG CTGAGGTGGA GAATGCCAAG
TGCCAGAATT CCAAGCTGGA GGCTGCGGTG GCTCAGTCTG AGCAGCAGGG TGAGGCGGCC
CTCAGCGATG CCCGCTGCAA GTTGGCCGAG CTGGAGGGTG CCCTGCAGAA GGCCAAGCAG
GACATGGCCT GCCTGATCAG GGAGTACCAG GAGGTGATGA ACTCCAAGCT GGGCCTGGAC
ATCGAGATCG CCACCTACAG GCGCCTGCTG GAGGGCGAGG AGCAGAGGCT GTGCGAGGGC
GTCGGCTCGG TGAATGTCTG CGTCAGCAGC TCCCGCGGTG GCGTTGTCTG TGGCGATCTC
TGCGCCTCCA CTACTGCCCC TGTTGTCTCC ACCAGAGTCA GTAGCGTCCC CAGCAACAGC
AACGTGGTGG TGGGCACTAC TAACGCCTGC GCCCCCTCCG CCCGGGTTGG CGTCTGCGGC
GGCAGCTGTA AGAGGTGCTA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011536638.1
CDS319..1779
Misc Feature(1)631..1566(+)
Misc Feature(2)991..1338(+)
Translation
Position Chain Variation Link
14 14 a, g dbSNP:77386533
18 18 c, t dbSNP:752367873
100 100 a, g dbSNP:535801778
132 132 a, c dbSNP:777133603
142 142 c, g dbSNP:758997917
156 156 g, t dbSNP:769306501
160 160 a, g dbSNP:556110486
175 175 c, t dbSNP:764757946
176 176 a, g dbSNP:568650704
209 209 c, t dbSNP:140173379
279 279 c, t dbSNP:558242010
287 287 ca, gg dbSNP:35306692
287 287 c, g dbSNP:11834151
288 288 a, g dbSNP:11834152
308 308 a, g dbSNP:145174534
318 318 c, g dbSNP:775628307
322 322 a, t dbSNP:762980858
327 327 g, t dbSNP:764023107
328 328 a, g dbSNP:751348438
330 330 a, c dbSNP:756434438
332 332 a, c dbSNP:766555284
334 334 c, t dbSNP:117662922
339 339 g, t dbSNP:755139437
345 345 a, c, g, t dbSNP:201329364
346 346 a, c dbSNP:758877634
348 348 c, t dbSNP:368082897
350 350 a, c dbSNP:747337849
351 351 c, t dbSNP:371864554
355 355 a, g dbSNP:780844187
356 356 c, g dbSNP:565104845
361 361 a, g, t dbSNP:530949768
366 366 c, g dbSNP:199538989
370 370 g, t dbSNP:550585959
372 372 c, t dbSNP:74651927
373 373 a, g dbSNP:774291422
378 378 c, g, t dbSNP:201694571
380 380 a, c, g dbSNP:754153541
382 382 -, c dbSNP:745354445
384 384 c, g dbSNP:765348086
385 385 a, g, t dbSNP:752727601
388 388 a, c dbSNP:778190510
395 395 a, g dbSNP:751938228
396 396 c, t dbSNP:201420491
402 402 c, t dbSNP:781406879
404 404 c, t dbSNP:566680440
405 405 a, c, t dbSNP:112261068
410 410 c, g dbSNP:748739670
411 411 c, g dbSNP:768727542
415 415 c, t dbSNP:774487692
416 416 a, c, g, t dbSNP:552266351
417 417 c, t dbSNP:772858714
420 420 c, t dbSNP:759824354
423 423 c, g dbSNP:765405708
425 425 a, c dbSNP:202141424
426 426 c, t dbSNP:762965684
433 433 c, t dbSNP:764149570
434 434 a, g dbSNP:752176981
435 435 c, t dbSNP:757675096
441 441 a, c, t dbSNP:781658784
444 444 c, t dbSNP:755794129
445 445 c, g dbSNP:779794188
446 446 a, g dbSNP:748935718
447 447 c, g dbSNP:754598294
453 453 c, t dbSNP:778531514
455 455 c, g dbSNP:748162043
462 462 c, t dbSNP:771852441
466 466 a, g, t dbSNP:773093741
472 472 c, g dbSNP:376835872
473 473 a, g dbSNP:557368421
479 479 c, t dbSNP:574036785
481 481 c, t dbSNP:764200311
482 482 a, g dbSNP:774273613
486 486 c, t dbSNP:762407305
487 487 c, g, t dbSNP:369728157
490 490 g, t dbSNP:756604025
494 494 -, ctt dbSNP:775534639
495 495 c, t dbSNP:766845192
497 497 -, gacgca dbSNP:760642690
499 499 c, g dbSNP:753581743
507 507 c, t dbSNP:754651481
508 508 c, g dbSNP:778504570
510 510 a, c dbSNP:747568260
513 513 -, c dbSNP:768654938
514 514 a, c dbSNP:758364190
515 515 a, g dbSNP:57242951
519 519 c, t dbSNP:553105016
520 520 a, c, g dbSNP:573121340
522 522 c, g dbSNP:749564887
523 523 a, g dbSNP:768820064
540 540 c, t dbSNP:774523262
541 541 a, c dbSNP:761738178
542 542 c, t dbSNP:767618159
544 544 c, g dbSNP:773655908
550 550 a, g dbSNP:761233503
558 558 a, c dbSNP:766898287
564 564 a, g dbSNP:754205786
584 584 c, t dbSNP:754706890
593 593 a, g dbSNP:764732778
606 606 c, t dbSNP:545137193
612 612 c, t dbSNP:757904259
614 614 c, t dbSNP:777333199
615 615 a, g, t dbSNP:199751260
619 619 g, t dbSNP:780990129
622 622 a, g dbSNP:745624392
623 623 c, t dbSNP:768877244
652 652 -, ccctcaac dbSNP:776225547
658 658 a, c, g dbSNP:61091894
666 666 a, g dbSNP:58580222
670 670 a, g dbSNP:748394653
671 671 a, c dbSNP:60612575
715 715 a, c dbSNP:537434322
733 733 a, c dbSNP:771522388
734 734 a, c dbSNP:58717266
809 809 a, g dbSNP:548127873
823 823 g, t dbSNP:567721642
954 954 a, g dbSNP:79398941
959 959 a, g dbSNP:56677856
961 961 a, g dbSNP:781043236
967 967 a, t dbSNP:779212628
969 969 c, t dbSNP:377005978
979 979 c, t dbSNP:199678774
980 980 a, g, t dbSNP:531934335
983 983 a, t dbSNP:772288102
984 984 a, g dbSNP:201521867
991 991 a, c dbSNP:747016737
995 995 a, t dbSNP:771581226
997 997 a, g dbSNP:777336849
1000 1000 a, g dbSNP:760117096
1002 1002 c, t dbSNP:200750977
1003 1003 a, g dbSNP:775905498
1010 1010 a, c, t dbSNP:762539212
1016 1016 a, t dbSNP:751075241
1022 1022 a, g dbSNP:761368875
1023 1023 g, t dbSNP:200085173
1026 1026 c, t dbSNP:778154101
1027 1027 a, g dbSNP:756052236
1037 1037 a, g dbSNP:201433700
1039 1039 c, t dbSNP:111429470
1040 1040 a, g dbSNP:753627634
1041 1041 a, g dbSNP:758552461
1044 1044 a, g dbSNP:778081882
1045 1045 c, t dbSNP:759397233
1046 1046 a, c, g, t dbSNP:202150817
1050 1050 a, c, g dbSNP:78831098
1051 1051 a, g dbSNP:775752662
1058 1058 g, t dbSNP:776699267
1060 1060 c, t dbSNP:368604832
1061 1061 a, g, t dbSNP:765173749
1062 1062 c, t dbSNP:58523335
1063 1063 a, g dbSNP:61914259
1070 1070 a, g dbSNP:750485674
1071 1071 a, g dbSNP:756277678
1073 1073 c, t dbSNP:779962943
1074 1074 c, g, t dbSNP:61915660
1082 1082 c, t dbSNP:779276684
1083 1083 a, g dbSNP:748444485
1084 1084 g, t dbSNP:367712000
1086 1086 c, t dbSNP:371492790
1089 1089 c, g dbSNP:112956699
1092 1092 c, g dbSNP:770517793
1093 1093 a, g dbSNP:369679589
1096 1096 a, g, t dbSNP:759569087
1117 1117 c, t dbSNP:527924718
1118 1118 a, g dbSNP:371922719
1130 1130 c, g, t dbSNP:763786271
1138 1138 a, g dbSNP:760783190
1141 1141 a, g dbSNP:756033789
1146 1146 c, t dbSNP:200472524
1147 1147 a, g dbSNP:376581340
1151 1151 g, t dbSNP:755479412
1152 1152 c, t dbSNP:779333951
1159 1159 a, c dbSNP:753187461
1160 1160 a, c, t dbSNP:369890550
1164 1164 c, t dbSNP:777969989
1165 1165 a, g dbSNP:377327853
1171 1171 a, t dbSNP:770441867
1174 1174 a, g, t dbSNP:780785303
1180 1180 c, g, t dbSNP:199724105
1181 1181 a, g dbSNP:376573652
1182 1182 c, t dbSNP:768542333
1186 1186 c, t dbSNP:762794979
1187 1187 a, g dbSNP:370858159
1190 1190 c, g dbSNP:766441670
1191 1191 c, t dbSNP:190756867
1192 1192 a, g dbSNP:759668482
1195 1195 a, c, g dbSNP:765295167
1196 1196 a, c dbSNP:376319970
1197 1197 c, t dbSNP:569717946
1198 1198 a, g, t dbSNP:183358379
1202 1202 c, g dbSNP:780655613
1209 1209 c, t dbSNP:374553856
1210 1210 c, t dbSNP:201943606
1211 1211 a, g dbSNP:199840628
1212 1212 c, g dbSNP:534563196
1229 1229 c, t dbSNP:201760569
1234 1234 a, g dbSNP:762354129
1238 1238 c, t dbSNP:372547107
1239 1239 a, g dbSNP:750775368
1245 1245 c, g dbSNP:760403544
1251 1251 c, g, t dbSNP:144028073
1252 1252 a, g dbSNP:754512829
1260 1260 c, t dbSNP:778453366
1261 1261 c, t dbSNP:752624557
1262 1262 a, t dbSNP:758283340
1264 1264 a, c, t dbSNP:369280939
1265 1265 a, g dbSNP:770017878
1267 1267 c, t dbSNP:780315495
1268 1268 a, g dbSNP:557621183
1281 1281 a, g dbSNP:768661795
1283 1283 a, t dbSNP:774470730
1286 1286 a, g dbSNP:373358594
1287 1287 c, t dbSNP:772636597
1289 1289 a, g dbSNP:773722982
1296 1296 c, t dbSNP:376127132
1297 1297 c, t dbSNP:139895699
1298 1298 a, g dbSNP:753470822
1300 1300 a, c, g dbSNP:759203655
1302 1302 a, g dbSNP:752211932
1303 1303 a, t dbSNP:758338480
1305 1305 a, c dbSNP:777631631
1311 1311 a, g dbSNP:560252337
1317 1317 a, g dbSNP:374471358
1318 1318 c, g dbSNP:781014025
1320 1320 c, g, t dbSNP:2032360
1321 1321 a, g dbSNP:376386930
1326 1326 a, g dbSNP:202026667
1329 1329 a, g dbSNP:772262266
1333 1333 c, g dbSNP:773921393
1341 1341 c, t dbSNP:747486507
1342 1342 a, c dbSNP:771471186
1347 1347 c, t dbSNP:763647926
1351 1351 a, t dbSNP:368204968
1352 1352 a, g, t dbSNP:372469887
1354 1354 c, t dbSNP:191545221
1357 1357 a, g dbSNP:756000963
1362 1362 c, t dbSNP:4761788
1364 1364 c, t dbSNP:201286632
1365 1365 a, g dbSNP:369312934
1368 1368 a, g dbSNP:778028956
1369 1369 a, g dbSNP:747018877
1371 1371 a, c, t dbSNP:28733260
1380 1380 g, t dbSNP:746358096
1384 1384 c, g, t dbSNP:770237949
1387 1387 c, g dbSNP:201484793
1394 1394 c, t dbSNP:534973333
1395 1395 a, g dbSNP:773861385
1397 1397 c, t dbSNP:202169108
1399 1399 c, g dbSNP:376312545
1404 1404 c, t dbSNP:11170086
1405 1405 a, g dbSNP:760506150
1409 1409 a, c dbSNP:766217821
1411 1411 c, t dbSNP:201833908
1412 1412 a, g dbSNP:373932045
1414 1414 c, t dbSNP:578093160
1420 1420 c, t dbSNP:11170087
1421 1421 c, t dbSNP:757425820
1425 1425 a, c, g, t dbSNP:376051219
1426 1426 a, g, t dbSNP:200353690
1429 1429 c, t dbSNP:749593452
1436 1436 g, t dbSNP:768991511
1437 1437 c, t dbSNP:11170088
1442 1442 c, t dbSNP:747720953
1448 1448 a, t dbSNP:771660500
1449 1449 g, t dbSNP:772546220
1463 1463 g, t dbSNP:760697549
1474 1474 a, c dbSNP:563550345
1477 1477 a, t dbSNP:776580728
1478 1478 a, g dbSNP:573751348
1482 1482 a, c, g dbSNP:375789021
1487 1487 a, g dbSNP:761921829
1496 1496 a, c, t dbSNP:767661038
1500 1500 c, t dbSNP:756653876
1505 1505 a, g dbSNP:542735645
1514 1514 c, t dbSNP:754335203
1521 1521 c, t dbSNP:755372953
1522 1522 a, c, g dbSNP:60687604
1527 1527 c, t dbSNP:747856782
1528 1528 a, g dbSNP:757946783
1530 1530 c, t dbSNP:777341221
1540 1540 c, t dbSNP:749369523
1541 1541 a, g dbSNP:199951808
1542 1542 c, t dbSNP:368480216
1546 1546 c, t dbSNP:370091437
1551 1551 a, g dbSNP:769763274
1553 1553 a, g dbSNP:775487582
1554 1554 a, c, t dbSNP:372174710
1555 1555 a, g dbSNP:121909129
1557 1557 g, t dbSNP:121909130
1578 1578 a, c dbSNP:749890373
1579 1579 a, g dbSNP:756138046
1582 1582 a, g dbSNP:779918036
1586 1586 a, c dbSNP:562353539
1587 1587 c, g dbSNP:768381688
1593 1593 c, t dbSNP:778718877
1597 1597 a, t dbSNP:747260525
1599 1599 a, c dbSNP:561311116
1600 1600 g, t dbSNP:763403025
1615 1615 a, g dbSNP:769143368
1620 1620 a, c, g dbSNP:527319461
1628 1628 c, g dbSNP:767957618
1640 1640 c, g dbSNP:773044010
1642 1642 a, g dbSNP:760323015
1643 1643 c, t dbSNP:765834889
1653 1653 a, t dbSNP:753350605
1659 1659 c, t dbSNP:754896113
1660 1660 a, c, g dbSNP:200098470
1670 1670 c, g dbSNP:758256483
1671 1671 c, t dbSNP:370419090
1672 1672 a, g dbSNP:746090200
1680 1680 c, t dbSNP:756291633
1684 1684 a, g dbSNP:780026281
1685 1685 a, t dbSNP:749337520
1688 1688 c, t dbSNP:768592222
1691 1691 a, g dbSNP:775033485
1692 1692 c, t dbSNP:748655954
1696 1696 a, g dbSNP:772581641
1704 1704 a, g dbSNP:773647506
1707 1707 c, g dbSNP:374122056
1711 1711 c, g dbSNP:560668616
1723 1723 a, g dbSNP:776126777
1726 1726 c, t dbSNP:532729838
1730 1730 c, t dbSNP:368279953
1733 1733 c, g dbSNP:752688759
1734 1734 a, c dbSNP:758272986
1736 1736 c, t dbSNP:763879273
1738 1738 g, t dbSNP:751326981
1742 1742 c, g, t dbSNP:756310592
1743 1743 g, t dbSNP:749435372
1745 1745 -, t dbSNP:752996118
1749 1749 a, c dbSNP:755171534
1750 1750 c, g, t dbSNP:779081505
1751 1751 c, t dbSNP:772669443
1756 1756 a, g dbSNP:773737109
1759 1759 a, c, g dbSNP:552762476
1766 1766 -, t dbSNP:756555632
1772 1772 a, g dbSNP:771169755
1775 1775 a, g dbSNP:201740931
1776 1776 c, t dbSNP:759097243
1777 1777 c, t dbSNP:769306504
1779 1779 a, g dbSNP:774913128
1782 1782 a, g dbSNP:148309398
1785 1785 g, t dbSNP:763968892
1789 1789 c, g dbSNP:751427480
1791 1791 a, c dbSNP:761704739
1795 1795 g, t dbSNP:548735427
1800 1800 a, c dbSNP:375430561
1801 1801 g, t dbSNP:755188054
1808 1808 a, g dbSNP:779167469
1810 1810 c, g dbSNP:752778941
1811 1811 c, g dbSNP:758531707
1821 1821 a, c dbSNP:568421074
1825 1825 a, c dbSNP:201257801
1838 1838 c, g dbSNP:771115534
1841 1841 c, t dbSNP:554027353
1873 1873 a, g dbSNP:2280949
1885 1885 c, g dbSNP:573713057
1903 1903 c, g dbSNP:559456695
1906 1906 c, t dbSNP:4076515
1940 1940 a, g dbSNP:768815810
1949 1949 -, g dbSNP:775440318
1988 1988 a, g dbSNP:185290279
1999 1999 a, g dbSNP:188381439
2006 2006 a, g dbSNP:548333894
2043 2043 a, g dbSNP:1137013
2050 2050 -, t, tt dbSNP:536030727
2051 2051 -, tt dbSNP:111847567
2058 2058 -, t, tt, ttt dbSNP:369883463
2059 2059 -, t dbSNP:3215437
2066 2066 a, g dbSNP:1137014
2091 2091 c, t dbSNP:1137015
2137 2137 a, g dbSNP:116249711
2196 2196 a, g dbSNP:532647627
2201 2201 c, t dbSNP:546535987
2221 2221 c, g dbSNP:563059388
2239 2239 a, g dbSNP:750820066
2249 2249 c, g dbSNP:141418442
2296 2296 a, c dbSNP:548699342
2312 2312 c, t dbSNP:767008402
2316 2316 a, g dbSNP:369975927
2351 2351 a, g dbSNP:527798405

Target ORF information:

RefSeq Version XM_011538336
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens keratin 86, type II (KRT86), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011538336

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACTTGTG GATCTTACTG TGGTGGCCGC GCCTTCAGCT GCATCTCGGC CTGCGGGCCC 
CGGCCCGGCC GCTGCTGCAT CACCGCCGCC CCCTACCGTG GCATCTCCTG CTACCGCGGC
CTCACCGGGG GCTTCGGCAG CCACAGCGTG TGCGGAGGCT TTCGGGCCGG CTCCTGCGGA
CGCAGCTTCG GCTACCGCTC CGGGGGCGTG TGCGGGCCCA GTCCCCCATG CATCACCACC
GTGTCGGTCA ACGAGAGCCT CCTCACGCCC CTCAACCTGG AGATCGACCC CAACGCGCAG
TGCGTGAAGC AGGAGGAGAA GGAGCAGATC AAGTCCCTCA ACAGCAGGTT CGCGGCCTTC
ATCGACAAGG TGCGCTTCCT GGAGCAGCAG AACAAACTGC TGGAGACAAA GCTGCAGTTC
TACCAGAACC GCGAGTGTTG CCAGAGCAAC CTGGAGCCCC TGTTTGAGGG CTACATCGAG
ACTCTGCGGC GGGAGGCCGA GTGCGTGGAG GCCGACAGCG GGAGGCTGGC CTCAGAGCTT
AACCACGTGC AGGAGGTGCT GGAGGGCTAC AAGAAGAAGT ATGAGGAGGA GGTTTCTCTG
AGAGCAACAG CTGAGAACGA GTTTGTGGCT CTGAAGAAGG ATGTGGACTG CGCCTACCTC
CGCAAATCAG ACCTGGAGGC CAATGTGGAG GCCCTGATCC AGGAGATCGA CTTCCTGAGG
CGGCTGTATG AGGAGGAGAT CCGCGTTCTC CAGTCCCACA TCTCAGACAC CTCCGTGGTT
GTCAAGCTGG ACAACAGCCG GGACCTGAAC ATGGACTGCA TCATTGCCGA GATCAAGGCA
CAGTACGATG ACATTGTCAC CCGTAGCCGG GCTGAGGCCG AGTCCTGGTA CCGCAGCAAG
TGTGAGGAGA TGAAGGCCAC GGTGATCAGG CACGGGGAGA CCCTGCGCCG CACCAAGGAG
GAGATCAACG AGCTGAACCG CATGATCCAG AGGCTGACGG CTGAGGTGGA GAATGCCAAG
TGCCAGAATT CCAAGCTGGA GGCTGCGGTG GCTCAGTCTG AGCAGCAGGG TGAGGCGGCC
CTCAGCGATG CCCGCTGCAA GTTGGCCGAG CTGGAGGGTG CCCTGCAGAA GGCCAAGCAG
GACATGGCCT GCCTGATCAG GGAGTACCAG GAGGTGATGA ACTCCAAGCT GGGCCTGGAC
ATCGAGATCG CCACCTACAG GCGCCTGCTG GAGGGCGAGG AGCAGAGGCT GTGCGAGGGC
GTCGGCTCGG TGAATGTCTG CGTCAGCAGC TCCCGCGGTG GCGTTGTCTG TGGCGATCTC
TGCGCCTCCA CTACTGCCCC TGTTGTCTCC ACCAGAGTCA GTAGCGTCCC CAGCAACAGC
AACGTGGTGG TGGGCACTAC TAACGCCTGC GCCCCCTCCG CCCGGGTTGG CGTCTGCGGC
GGCAGCTGTA AGAGGTGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17915
Accession Version XM_011538337.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1461bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product keratin, type II cuticular Hb6 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACTTGTG GATCTTACTG TGGTGGCCGC GCCTTCAGCT GCATCTCGGC CTGCGGGCCC 
CGGCCCGGCC GCTGCTGCAT CACCGCCGCC CCCTACCGTG GCATCTCCTG CTACCGCGGC
CTCACCGGGG GCTTCGGCAG CCACAGCGTG TGCGGAGGCT TTCGGGCCGG CTCCTGCGGA
CGCAGCTTCG GCTACCGCTC CGGGGGCGTG TGCGGGCCCA GTCCCCCATG CATCACCACC
GTGTCGGTCA ACGAGAGCCT CCTCACGCCC CTCAACCTGG AGATCGACCC CAACGCGCAG
TGCGTGAAGC AGGAGGAGAA GGAGCAGATC AAGTCCCTCA ACAGCAGGTT CGCGGCCTTC
ATCGACAAGG TGCGCTTCCT GGAGCAGCAG AACAAACTGC TGGAGACAAA GCTGCAGTTC
TACCAGAACC GCGAGTGTTG CCAGAGCAAC CTGGAGCCCC TGTTTGAGGG CTACATCGAG
ACTCTGCGGC GGGAGGCCGA GTGCGTGGAG GCCGACAGCG GGAGGCTGGC CTCAGAGCTT
AACCACGTGC AGGAGGTGCT GGAGGGCTAC AAGAAGAAGT ATGAGGAGGA GGTTTCTCTG
AGAGCAACAG CTGAGAACGA GTTTGTGGCT CTGAAGAAGG ATGTGGACTG CGCCTACCTC
CGCAAATCAG ACCTGGAGGC CAATGTGGAG GCCCTGATCC AGGAGATCGA CTTCCTGAGG
CGGCTGTATG AGGAGGAGAT CCGCGTTCTC CAGTCCCACA TCTCAGACAC CTCCGTGGTT
GTCAAGCTGG ACAACAGCCG GGACCTGAAC ATGGACTGCA TCATTGCCGA GATCAAGGCA
CAGTACGATG ACATTGTCAC CCGTAGCCGG GCTGAGGCCG AGTCCTGGTA CCGCAGCAAG
TGTGAGGAGA TGAAGGCCAC GGTGATCAGG CACGGGGAGA CCCTGCGCCG CACCAAGGAG
GAGATCAACG AGCTGAACCG CATGATCCAG AGGCTGACGG CTGAGGTGGA GAATGCCAAG
TGCCAGAATT CCAAGCTGGA GGCTGCGGTG GCTCAGTCTG AGCAGCAGGG TGAGGCGGCC
CTCAGCGATG CCCGCTGCAA GTTGGCCGAG CTGGAGGGTG CCCTGCAGAA GGCCAAGCAG
GACATGGCCT GCCTGATCAG GGAGTACCAG GAGGTGATGA ACTCCAAGCT GGGCCTGGAC
ATCGAGATCG CCACCTACAG GCGCCTGCTG GAGGGCGAGG AGCAGAGGCT GTGCGAGGGC
GTCGGCTCGG TGAATGTCTG CGTCAGCAGC TCCCGCGGTG GCGTTGTCTG TGGCGATCTC
TGCGCCTCCA CTACTGCCCC TGTTGTCTCC ACCAGAGTCA GTAGCGTCCC CAGCAACAGC
AACGTGGTGG TGGGCACTAC TAACGCCTGC GCCCCCTCCG CCCGGGTTGG CGTCTGCGGC
GGCAGCTGTA AGAGGTGCTA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011536639.1
CDS205..1665
Misc Feature(1)517..1452(+)
Misc Feature(2)877..1224(+)
Translation
Position Chain Variation Link
18 18 a, g dbSNP:77386533
22 22 c, t dbSNP:752367873
95 95 c, t dbSNP:140173379
165 165 c, t dbSNP:558242010
173 173 ca, gg dbSNP:35306692
173 173 c, g dbSNP:11834151
174 174 a, g dbSNP:11834152
194 194 a, g dbSNP:145174534
204 204 c, g dbSNP:775628307
208 208 a, t dbSNP:762980858
213 213 g, t dbSNP:764023107
214 214 a, g dbSNP:751348438
216 216 a, c dbSNP:756434438
218 218 a, c dbSNP:766555284
220 220 c, t dbSNP:117662922
225 225 g, t dbSNP:755139437
231 231 a, c, g, t dbSNP:201329364
232 232 a, c dbSNP:758877634
234 234 c, t dbSNP:368082897
236 236 a, c dbSNP:747337849
237 237 c, t dbSNP:371864554
241 241 a, g dbSNP:780844187
242 242 c, g dbSNP:565104845
247 247 a, g, t dbSNP:530949768
252 252 c, g dbSNP:199538989
256 256 g, t dbSNP:550585959
258 258 c, t dbSNP:74651927
259 259 a, g dbSNP:774291422
264 264 c, g, t dbSNP:201694571
266 266 a, c, g dbSNP:754153541
268 268 -, c dbSNP:745354445
270 270 c, g dbSNP:765348086
271 271 a, g, t dbSNP:752727601
274 274 a, c dbSNP:778190510
281 281 a, g dbSNP:751938228
282 282 c, t dbSNP:201420491
288 288 c, t dbSNP:781406879
290 290 c, t dbSNP:566680440
291 291 a, c, t dbSNP:112261068
296 296 c, g dbSNP:748739670
297 297 c, g dbSNP:768727542
301 301 c, t dbSNP:774487692
302 302 a, c, g, t dbSNP:552266351
303 303 c, t dbSNP:772858714
306 306 c, t dbSNP:759824354
309 309 c, g dbSNP:765405708
311 311 a, c dbSNP:202141424
312 312 c, t dbSNP:762965684
319 319 c, t dbSNP:764149570
320 320 a, g dbSNP:752176981
321 321 c, t dbSNP:757675096
327 327 a, c, t dbSNP:781658784
330 330 c, t dbSNP:755794129
331 331 c, g dbSNP:779794188
332 332 a, g dbSNP:748935718
333 333 c, g dbSNP:754598294
339 339 c, t dbSNP:778531514
341 341 c, g dbSNP:748162043
348 348 c, t dbSNP:771852441
352 352 a, g, t dbSNP:773093741
358 358 c, g dbSNP:376835872
359 359 a, g dbSNP:557368421
365 365 c, t dbSNP:574036785
367 367 c, t dbSNP:764200311
368 368 a, g dbSNP:774273613
372 372 c, t dbSNP:762407305
373 373 c, g, t dbSNP:369728157
376 376 g, t dbSNP:756604025
380 380 -, ctt dbSNP:775534639
381 381 c, t dbSNP:766845192
383 383 -, gacgca dbSNP:760642690
385 385 c, g dbSNP:753581743
393 393 c, t dbSNP:754651481
394 394 c, g dbSNP:778504570
396 396 a, c dbSNP:747568260
399 399 -, c dbSNP:768654938
400 400 a, c dbSNP:758364190
401 401 a, g dbSNP:57242951
405 405 c, t dbSNP:553105016
406 406 a, c, g dbSNP:573121340
408 408 c, g dbSNP:749564887
409 409 a, g dbSNP:768820064
426 426 c, t dbSNP:774523262
427 427 a, c dbSNP:761738178
428 428 c, t dbSNP:767618159
430 430 c, g dbSNP:773655908
436 436 a, g dbSNP:761233503
444 444 a, c dbSNP:766898287
450 450 a, g dbSNP:754205786
470 470 c, t dbSNP:754706890
479 479 a, g dbSNP:764732778
492 492 c, t dbSNP:545137193
498 498 c, t dbSNP:757904259
500 500 c, t dbSNP:777333199
501 501 a, g, t dbSNP:199751260
505 505 g, t dbSNP:780990129
508 508 a, g dbSNP:745624392
509 509 c, t dbSNP:768877244
538 538 -, ccctcaac dbSNP:776225547
544 544 a, c, g dbSNP:61091894
552 552 a, g dbSNP:58580222
556 556 a, g dbSNP:748394653
557 557 a, c dbSNP:60612575
601 601 a, c dbSNP:537434322
619 619 a, c dbSNP:771522388
620 620 a, c dbSNP:58717266
695 695 a, g dbSNP:548127873
709 709 g, t dbSNP:567721642
840 840 a, g dbSNP:79398941
845 845 a, g dbSNP:56677856
847 847 a, g dbSNP:781043236
853 853 a, t dbSNP:779212628
855 855 c, t dbSNP:377005978
865 865 c, t dbSNP:199678774
866 866 a, g, t dbSNP:531934335
869 869 a, t dbSNP:772288102
870 870 a, g dbSNP:201521867
877 877 a, c dbSNP:747016737
881 881 a, t dbSNP:771581226
883 883 a, g dbSNP:777336849
886 886 a, g dbSNP:760117096
888 888 c, t dbSNP:200750977
889 889 a, g dbSNP:775905498
896 896 a, c, t dbSNP:762539212
902 902 a, t dbSNP:751075241
908 908 a, g dbSNP:761368875
909 909 g, t dbSNP:200085173
912 912 c, t dbSNP:778154101
913 913 a, g dbSNP:756052236
923 923 a, g dbSNP:201433700
925 925 c, t dbSNP:111429470
926 926 a, g dbSNP:753627634
927 927 a, g dbSNP:758552461
930 930 a, g dbSNP:778081882
931 931 c, t dbSNP:759397233
932 932 a, c, g, t dbSNP:202150817
936 936 a, c, g dbSNP:78831098
937 937 a, g dbSNP:775752662
944 944 g, t dbSNP:776699267
946 946 c, t dbSNP:368604832
947 947 a, g, t dbSNP:765173749
948 948 c, t dbSNP:58523335
949 949 a, g dbSNP:61914259
956 956 a, g dbSNP:750485674
957 957 a, g dbSNP:756277678
959 959 c, t dbSNP:779962943
960 960 c, g, t dbSNP:61915660
968 968 c, t dbSNP:779276684
969 969 a, g dbSNP:748444485
970 970 g, t dbSNP:367712000
972 972 c, t dbSNP:371492790
975 975 c, g dbSNP:112956699
978 978 c, g dbSNP:770517793
979 979 a, g dbSNP:369679589
982 982 a, g, t dbSNP:759569087
1003 1003 c, t dbSNP:527924718
1004 1004 a, g dbSNP:371922719
1016 1016 c, g, t dbSNP:763786271
1024 1024 a, g dbSNP:760783190
1027 1027 a, g dbSNP:756033789
1032 1032 c, t dbSNP:200472524
1033 1033 a, g dbSNP:376581340
1037 1037 g, t dbSNP:755479412
1038 1038 c, t dbSNP:779333951
1045 1045 a, c dbSNP:753187461
1046 1046 a, c, t dbSNP:369890550
1050 1050 c, t dbSNP:777969989
1051 1051 a, g dbSNP:377327853
1057 1057 a, t dbSNP:770441867
1060 1060 a, g, t dbSNP:780785303
1066 1066 c, g, t dbSNP:199724105
1067 1067 a, g dbSNP:376573652
1068 1068 c, t dbSNP:768542333
1072 1072 c, t dbSNP:762794979
1073 1073 a, g dbSNP:370858159
1076 1076 c, g dbSNP:766441670
1077 1077 c, t dbSNP:190756867
1078 1078 a, g dbSNP:759668482
1081 1081 a, c, g dbSNP:765295167
1082 1082 a, c dbSNP:376319970
1083 1083 c, t dbSNP:569717946
1084 1084 a, g, t dbSNP:183358379
1088 1088 c, g dbSNP:780655613
1095 1095 c, t dbSNP:374553856
1096 1096 c, t dbSNP:201943606
1097 1097 a, g dbSNP:199840628
1098 1098 c, g dbSNP:534563196
1115 1115 c, t dbSNP:201760569
1120 1120 a, g dbSNP:762354129
1124 1124 c, t dbSNP:372547107
1125 1125 a, g dbSNP:750775368
1131 1131 c, g dbSNP:760403544
1137 1137 c, g, t dbSNP:144028073
1138 1138 a, g dbSNP:754512829
1146 1146 c, t dbSNP:778453366
1147 1147 c, t dbSNP:752624557
1148 1148 a, t dbSNP:758283340
1150 1150 a, c, t dbSNP:369280939
1151 1151 a, g dbSNP:770017878
1153 1153 c, t dbSNP:780315495
1154 1154 a, g dbSNP:557621183
1167 1167 a, g dbSNP:768661795
1169 1169 a, t dbSNP:774470730
1172 1172 a, g dbSNP:373358594
1173 1173 c, t dbSNP:772636597
1175 1175 a, g dbSNP:773722982
1182 1182 c, t dbSNP:376127132
1183 1183 c, t dbSNP:139895699
1184 1184 a, g dbSNP:753470822
1186 1186 a, c, g dbSNP:759203655
1188 1188 a, g dbSNP:752211932
1189 1189 a, t dbSNP:758338480
1191 1191 a, c dbSNP:777631631
1197 1197 a, g dbSNP:560252337
1203 1203 a, g dbSNP:374471358
1204 1204 c, g dbSNP:781014025
1206 1206 c, g, t dbSNP:2032360
1207 1207 a, g dbSNP:376386930
1212 1212 a, g dbSNP:202026667
1215 1215 a, g dbSNP:772262266
1219 1219 c, g dbSNP:773921393
1227 1227 c, t dbSNP:747486507
1228 1228 a, c dbSNP:771471186
1233 1233 c, t dbSNP:763647926
1237 1237 a, t dbSNP:368204968
1238 1238 a, g, t dbSNP:372469887
1240 1240 c, t dbSNP:191545221
1243 1243 a, g dbSNP:756000963
1248 1248 c, t dbSNP:4761788
1250 1250 c, t dbSNP:201286632
1251 1251 a, g dbSNP:369312934
1254 1254 a, g dbSNP:778028956
1255 1255 a, g dbSNP:747018877
1257 1257 a, c, t dbSNP:28733260
1266 1266 g, t dbSNP:746358096
1270 1270 c, g, t dbSNP:770237949
1273 1273 c, g dbSNP:201484793
1280 1280 c, t dbSNP:534973333
1281 1281 a, g dbSNP:773861385
1283 1283 c, t dbSNP:202169108
1285 1285 c, g dbSNP:376312545
1290 1290 c, t dbSNP:11170086
1291 1291 a, g dbSNP:760506150
1295 1295 a, c dbSNP:766217821
1297 1297 c, t dbSNP:201833908
1298 1298 a, g dbSNP:373932045
1300 1300 c, t dbSNP:578093160
1306 1306 c, t dbSNP:11170087
1307 1307 c, t dbSNP:757425820
1311 1311 a, c, g, t dbSNP:376051219
1312 1312 a, g, t dbSNP:200353690
1315 1315 c, t dbSNP:749593452
1322 1322 g, t dbSNP:768991511
1323 1323 c, t dbSNP:11170088
1328 1328 c, t dbSNP:747720953
1334 1334 a, t dbSNP:771660500
1335 1335 g, t dbSNP:772546220
1349 1349 g, t dbSNP:760697549
1360 1360 a, c dbSNP:563550345
1363 1363 a, t dbSNP:776580728
1364 1364 a, g dbSNP:573751348
1368 1368 a, c, g dbSNP:375789021
1373 1373 a, g dbSNP:761921829
1382 1382 a, c, t dbSNP:767661038
1386 1386 c, t dbSNP:756653876
1391 1391 a, g dbSNP:542735645
1400 1400 c, t dbSNP:754335203
1407 1407 c, t dbSNP:755372953
1408 1408 a, c, g dbSNP:60687604
1413 1413 c, t dbSNP:747856782
1414 1414 a, g dbSNP:757946783
1416 1416 c, t dbSNP:777341221
1426 1426 c, t dbSNP:749369523
1427 1427 a, g dbSNP:199951808
1428 1428 c, t dbSNP:368480216
1432 1432 c, t dbSNP:370091437
1437 1437 a, g dbSNP:769763274
1439 1439 a, g dbSNP:775487582
1440 1440 a, c, t dbSNP:372174710
1441 1441 a, g dbSNP:121909129
1443 1443 g, t dbSNP:121909130
1464 1464 a, c dbSNP:749890373
1465 1465 a, g dbSNP:756138046
1468 1468 a, g dbSNP:779918036
1472 1472 a, c dbSNP:562353539
1473 1473 c, g dbSNP:768381688
1479 1479 c, t dbSNP:778718877
1483 1483 a, t dbSNP:747260525
1485 1485 a, c dbSNP:561311116
1486 1486 g, t dbSNP:763403025
1501 1501 a, g dbSNP:769143368
1506 1506 a, c, g dbSNP:527319461
1514 1514 c, g dbSNP:767957618
1526 1526 c, g dbSNP:773044010
1528 1528 a, g dbSNP:760323015
1529 1529 c, t dbSNP:765834889
1539 1539 a, t dbSNP:753350605
1545 1545 c, t dbSNP:754896113
1546 1546 a, c, g dbSNP:200098470
1556 1556 c, g dbSNP:758256483
1557 1557 c, t dbSNP:370419090
1558 1558 a, g dbSNP:746090200
1566 1566 c, t dbSNP:756291633
1570 1570 a, g dbSNP:780026281
1571 1571 a, t dbSNP:749337520
1574 1574 c, t dbSNP:768592222
1577 1577 a, g dbSNP:775033485
1578 1578 c, t dbSNP:748655954
1582 1582 a, g dbSNP:772581641
1590 1590 a, g dbSNP:773647506
1593 1593 c, g dbSNP:374122056
1597 1597 c, g dbSNP:560668616
1609 1609 a, g dbSNP:776126777
1612 1612 c, t dbSNP:532729838
1616 1616 c, t dbSNP:368279953
1619 1619 c, g dbSNP:752688759
1620 1620 a, c dbSNP:758272986
1622 1622 c, t dbSNP:763879273
1624 1624 g, t dbSNP:751326981
1628 1628 c, g, t dbSNP:756310592
1629 1629 g, t dbSNP:749435372
1631 1631 -, t dbSNP:752996118
1635 1635 a, c dbSNP:755171534
1636 1636 c, g, t dbSNP:779081505
1637 1637 c, t dbSNP:772669443
1642 1642 a, g dbSNP:773737109
1645 1645 a, c, g dbSNP:552762476
1652 1652 -, t dbSNP:756555632
1658 1658 a, g dbSNP:771169755
1661 1661 a, g dbSNP:201740931
1662 1662 c, t dbSNP:759097243
1663 1663 c, t dbSNP:769306504
1665 1665 a, g dbSNP:774913128
1668 1668 a, g dbSNP:148309398
1671 1671 g, t dbSNP:763968892
1675 1675 c, g dbSNP:751427480
1677 1677 a, c dbSNP:761704739
1681 1681 g, t dbSNP:548735427
1686 1686 a, c dbSNP:375430561
1687 1687 g, t dbSNP:755188054
1694 1694 a, g dbSNP:779167469
1696 1696 c, g dbSNP:752778941
1697 1697 c, g dbSNP:758531707
1707 1707 a, c dbSNP:568421074
1711 1711 a, c dbSNP:201257801
1724 1724 c, g dbSNP:771115534
1727 1727 c, t dbSNP:554027353
1759 1759 a, g dbSNP:2280949
1771 1771 c, g dbSNP:573713057
1789 1789 c, g dbSNP:559456695
1792 1792 c, t dbSNP:4076515
1826 1826 a, g dbSNP:768815810
1835 1835 -, g dbSNP:775440318
1874 1874 a, g dbSNP:185290279
1885 1885 a, g dbSNP:188381439
1892 1892 a, g dbSNP:548333894
1929 1929 a, g dbSNP:1137013
1936 1936 -, t, tt dbSNP:536030727
1937 1937 -, tt dbSNP:111847567
1944 1944 -, t, tt, ttt dbSNP:369883463
1945 1945 -, t dbSNP:3215437
1952 1952 a, g dbSNP:1137014
1977 1977 c, t dbSNP:1137015
2023 2023 a, g dbSNP:116249711
2082 2082 a, g dbSNP:532647627
2087 2087 c, t dbSNP:546535987
2107 2107 c, g dbSNP:563059388
2125 2125 a, g dbSNP:750820066
2135 2135 c, g dbSNP:141418442
2182 2182 a, c dbSNP:548699342
2198 2198 c, t dbSNP:767008402
2202 2202 a, g dbSNP:369975927
2237 2237 a, g dbSNP:527798405

Target ORF information:

RefSeq Version XM_011538337
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens keratin 86, type II (KRT86), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011538337

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACTTGTG GATCTTACTG TGGTGGCCGC GCCTTCAGCT GCATCTCGGC CTGCGGGCCC 
CGGCCCGGCC GCTGCTGCAT CACCGCCGCC CCCTACCGTG GCATCTCCTG CTACCGCGGC
CTCACCGGGG GCTTCGGCAG CCACAGCGTG TGCGGAGGCT TTCGGGCCGG CTCCTGCGGA
CGCAGCTTCG GCTACCGCTC CGGGGGCGTG TGCGGGCCCA GTCCCCCATG CATCACCACC
GTGTCGGTCA ACGAGAGCCT CCTCACGCCC CTCAACCTGG AGATCGACCC CAACGCGCAG
TGCGTGAAGC AGGAGGAGAA GGAGCAGATC AAGTCCCTCA ACAGCAGGTT CGCGGCCTTC
ATCGACAAGG TGCGCTTCCT GGAGCAGCAG AACAAACTGC TGGAGACAAA GCTGCAGTTC
TACCAGAACC GCGAGTGTTG CCAGAGCAAC CTGGAGCCCC TGTTTGAGGG CTACATCGAG
ACTCTGCGGC GGGAGGCCGA GTGCGTGGAG GCCGACAGCG GGAGGCTGGC CTCAGAGCTT
AACCACGTGC AGGAGGTGCT GGAGGGCTAC AAGAAGAAGT ATGAGGAGGA GGTTTCTCTG
AGAGCAACAG CTGAGAACGA GTTTGTGGCT CTGAAGAAGG ATGTGGACTG CGCCTACCTC
CGCAAATCAG ACCTGGAGGC CAATGTGGAG GCCCTGATCC AGGAGATCGA CTTCCTGAGG
CGGCTGTATG AGGAGGAGAT CCGCGTTCTC CAGTCCCACA TCTCAGACAC CTCCGTGGTT
GTCAAGCTGG ACAACAGCCG GGACCTGAAC ATGGACTGCA TCATTGCCGA GATCAAGGCA
CAGTACGATG ACATTGTCAC CCGTAGCCGG GCTGAGGCCG AGTCCTGGTA CCGCAGCAAG
TGTGAGGAGA TGAAGGCCAC GGTGATCAGG CACGGGGAGA CCCTGCGCCG CACCAAGGAG
GAGATCAACG AGCTGAACCG CATGATCCAG AGGCTGACGG CTGAGGTGGA GAATGCCAAG
TGCCAGAATT CCAAGCTGGA GGCTGCGGTG GCTCAGTCTG AGCAGCAGGG TGAGGCGGCC
CTCAGCGATG CCCGCTGCAA GTTGGCCGAG CTGGAGGGTG CCCTGCAGAA GGCCAAGCAG
GACATGGCCT GCCTGATCAG GGAGTACCAG GAGGTGATGA ACTCCAAGCT GGGCCTGGAC
ATCGAGATCG CCACCTACAG GCGCCTGCTG GAGGGCGAGG AGCAGAGGCT GTGCGAGGGC
GTCGGCTCGG TGAATGTCTG CGTCAGCAGC TCCCGCGGTG GCGTTGTCTG TGGCGATCTC
TGCGCCTCCA CTACTGCCCC TGTTGTCTCC ACCAGAGTCA GTAGCGTCCC CAGCAACAGC
AACGTGGTGG TGGGCACTAC TAACGCCTGC GCCCCCTCCG CCCGGGTTGG CGTCTGCGGC
GGCAGCTGTA AGAGGTGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17915
Accession Version XM_011538338.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1461bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product keratin, type II cuticular Hb6 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACTTGTG GATCTTACTG TGGTGGCCGC GCCTTCAGCT GCATCTCGGC CTGCGGGCCC 
CGGCCCGGCC GCTGCTGCAT CACCGCCGCC CCCTACCGTG GCATCTCCTG CTACCGCGGC
CTCACCGGGG GCTTCGGCAG CCACAGCGTG TGCGGAGGCT TTCGGGCCGG CTCCTGCGGA
CGCAGCTTCG GCTACCGCTC CGGGGGCGTG TGCGGGCCCA GTCCCCCATG CATCACCACC
GTGTCGGTCA ACGAGAGCCT CCTCACGCCC CTCAACCTGG AGATCGACCC CAACGCGCAG
TGCGTGAAGC AGGAGGAGAA GGAGCAGATC AAGTCCCTCA ACAGCAGGTT CGCGGCCTTC
ATCGACAAGG TGCGCTTCCT GGAGCAGCAG AACAAACTGC TGGAGACAAA GCTGCAGTTC
TACCAGAACC GCGAGTGTTG CCAGAGCAAC CTGGAGCCCC TGTTTGAGGG CTACATCGAG
ACTCTGCGGC GGGAGGCCGA GTGCGTGGAG GCCGACAGCG GGAGGCTGGC CTCAGAGCTT
AACCACGTGC AGGAGGTGCT GGAGGGCTAC AAGAAGAAGT ATGAGGAGGA GGTTTCTCTG
AGAGCAACAG CTGAGAACGA GTTTGTGGCT CTGAAGAAGG ATGTGGACTG CGCCTACCTC
CGCAAATCAG ACCTGGAGGC CAATGTGGAG GCCCTGATCC AGGAGATCGA CTTCCTGAGG
CGGCTGTATG AGGAGGAGAT CCGCGTTCTC CAGTCCCACA TCTCAGACAC CTCCGTGGTT
GTCAAGCTGG ACAACAGCCG GGACCTGAAC ATGGACTGCA TCATTGCCGA GATCAAGGCA
CAGTACGATG ACATTGTCAC CCGTAGCCGG GCTGAGGCCG AGTCCTGGTA CCGCAGCAAG
TGTGAGGAGA TGAAGGCCAC GGTGATCAGG CACGGGGAGA CCCTGCGCCG CACCAAGGAG
GAGATCAACG AGCTGAACCG CATGATCCAG AGGCTGACGG CTGAGGTGGA GAATGCCAAG
TGCCAGAATT CCAAGCTGGA GGCTGCGGTG GCTCAGTCTG AGCAGCAGGG TGAGGCGGCC
CTCAGCGATG CCCGCTGCAA GTTGGCCGAG CTGGAGGGTG CCCTGCAGAA GGCCAAGCAG
GACATGGCCT GCCTGATCAG GGAGTACCAG GAGGTGATGA ACTCCAAGCT GGGCCTGGAC
ATCGAGATCG CCACCTACAG GCGCCTGCTG GAGGGCGAGG AGCAGAGGCT GTGCGAGGGC
GTCGGCTCGG TGAATGTCTG CGTCAGCAGC TCCCGCGGTG GCGTTGTCTG TGGCGATCTC
TGCGCCTCCA CTACTGCCCC TGTTGTCTCC ACCAGAGTCA GTAGCGTCCC CAGCAACAGC
AACGTGGTGG TGGGCACTAC TAACGCCTGC GCCCCCTCCG CCCGGGTTGG CGTCTGCGGC
GGCAGCTGTA AGAGGTGCTA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011536640.1
CDS231..1691
Misc Feature(1)543..1478(+)
Misc Feature(2)903..1250(+)
Translation
Position Chain Variation Link
15 15 a, g dbSNP:77386533
19 19 c, t dbSNP:752367873
121 121 c, t dbSNP:140173379
191 191 c, t dbSNP:558242010
199 199 ca, gg dbSNP:35306692
199 199 c, g dbSNP:11834151
200 200 a, g dbSNP:11834152
220 220 a, g dbSNP:145174534
230 230 c, g dbSNP:775628307
234 234 a, t dbSNP:762980858
239 239 g, t dbSNP:764023107
240 240 a, g dbSNP:751348438
242 242 a, c dbSNP:756434438
244 244 a, c dbSNP:766555284
246 246 c, t dbSNP:117662922
251 251 g, t dbSNP:755139437
257 257 a, c, g, t dbSNP:201329364
258 258 a, c dbSNP:758877634
260 260 c, t dbSNP:368082897
262 262 a, c dbSNP:747337849
263 263 c, t dbSNP:371864554
267 267 a, g dbSNP:780844187
268 268 c, g dbSNP:565104845
273 273 a, g, t dbSNP:530949768
278 278 c, g dbSNP:199538989
282 282 g, t dbSNP:550585959
284 284 c, t dbSNP:74651927
285 285 a, g dbSNP:774291422
290 290 c, g, t dbSNP:201694571
292 292 a, c, g dbSNP:754153541
294 294 -, c dbSNP:745354445
296 296 c, g dbSNP:765348086
297 297 a, g, t dbSNP:752727601
300 300 a, c dbSNP:778190510
307 307 a, g dbSNP:751938228
308 308 c, t dbSNP:201420491
314 314 c, t dbSNP:781406879
316 316 c, t dbSNP:566680440
317 317 a, c, t dbSNP:112261068
322 322 c, g dbSNP:748739670
323 323 c, g dbSNP:768727542
327 327 c, t dbSNP:774487692
328 328 a, c, g, t dbSNP:552266351
329 329 c, t dbSNP:772858714
332 332 c, t dbSNP:759824354
335 335 c, g dbSNP:765405708
337 337 a, c dbSNP:202141424
338 338 c, t dbSNP:762965684
345 345 c, t dbSNP:764149570
346 346 a, g dbSNP:752176981
347 347 c, t dbSNP:757675096
353 353 a, c, t dbSNP:781658784
356 356 c, t dbSNP:755794129
357 357 c, g dbSNP:779794188
358 358 a, g dbSNP:748935718
359 359 c, g dbSNP:754598294
365 365 c, t dbSNP:778531514
367 367 c, g dbSNP:748162043
374 374 c, t dbSNP:771852441
378 378 a, g, t dbSNP:773093741
384 384 c, g dbSNP:376835872
385 385 a, g dbSNP:557368421
391 391 c, t dbSNP:574036785
393 393 c, t dbSNP:764200311
394 394 a, g dbSNP:774273613
398 398 c, t dbSNP:762407305
399 399 c, g, t dbSNP:369728157
402 402 g, t dbSNP:756604025
406 406 -, ctt dbSNP:775534639
407 407 c, t dbSNP:766845192
409 409 -, gacgca dbSNP:760642690
411 411 c, g dbSNP:753581743
419 419 c, t dbSNP:754651481
420 420 c, g dbSNP:778504570
422 422 a, c dbSNP:747568260
425 425 -, c dbSNP:768654938
426 426 a, c dbSNP:758364190
427 427 a, g dbSNP:57242951
431 431 c, t dbSNP:553105016
432 432 a, c, g dbSNP:573121340
434 434 c, g dbSNP:749564887
435 435 a, g dbSNP:768820064
452 452 c, t dbSNP:774523262
453 453 a, c dbSNP:761738178
454 454 c, t dbSNP:767618159
456 456 c, g dbSNP:773655908
462 462 a, g dbSNP:761233503
470 470 a, c dbSNP:766898287
476 476 a, g dbSNP:754205786
496 496 c, t dbSNP:754706890
505 505 a, g dbSNP:764732778
518 518 c, t dbSNP:545137193
524 524 c, t dbSNP:757904259
526 526 c, t dbSNP:777333199
527 527 a, g, t dbSNP:199751260
531 531 g, t dbSNP:780990129
534 534 a, g dbSNP:745624392
535 535 c, t dbSNP:768877244
564 564 -, ccctcaac dbSNP:776225547
570 570 a, c, g dbSNP:61091894
578 578 a, g dbSNP:58580222
582 582 a, g dbSNP:748394653
583 583 a, c dbSNP:60612575
627 627 a, c dbSNP:537434322
645 645 a, c dbSNP:771522388
646 646 a, c dbSNP:58717266
721 721 a, g dbSNP:548127873
735 735 g, t dbSNP:567721642
866 866 a, g dbSNP:79398941
871 871 a, g dbSNP:56677856
873 873 a, g dbSNP:781043236
879 879 a, t dbSNP:779212628
881 881 c, t dbSNP:377005978
891 891 c, t dbSNP:199678774
892 892 a, g, t dbSNP:531934335
895 895 a, t dbSNP:772288102
896 896 a, g dbSNP:201521867
903 903 a, c dbSNP:747016737
907 907 a, t dbSNP:771581226
909 909 a, g dbSNP:777336849
912 912 a, g dbSNP:760117096
914 914 c, t dbSNP:200750977
915 915 a, g dbSNP:775905498
922 922 a, c, t dbSNP:762539212
928 928 a, t dbSNP:751075241
934 934 a, g dbSNP:761368875
935 935 g, t dbSNP:200085173
938 938 c, t dbSNP:778154101
939 939 a, g dbSNP:756052236
949 949 a, g dbSNP:201433700
951 951 c, t dbSNP:111429470
952 952 a, g dbSNP:753627634
953 953 a, g dbSNP:758552461
956 956 a, g dbSNP:778081882
957 957 c, t dbSNP:759397233
958 958 a, c, g, t dbSNP:202150817
962 962 a, c, g dbSNP:78831098
963 963 a, g dbSNP:775752662
970 970 g, t dbSNP:776699267
972 972 c, t dbSNP:368604832
973 973 a, g, t dbSNP:765173749
974 974 c, t dbSNP:58523335
975 975 a, g dbSNP:61914259
982 982 a, g dbSNP:750485674
983 983 a, g dbSNP:756277678
985 985 c, t dbSNP:779962943
986 986 c, g, t dbSNP:61915660
994 994 c, t dbSNP:779276684
995 995 a, g dbSNP:748444485
996 996 g, t dbSNP:367712000
998 998 c, t dbSNP:371492790
1001 1001 c, g dbSNP:112956699
1004 1004 c, g dbSNP:770517793
1005 1005 a, g dbSNP:369679589
1008 1008 a, g, t dbSNP:759569087
1029 1029 c, t dbSNP:527924718
1030 1030 a, g dbSNP:371922719
1042 1042 c, g, t dbSNP:763786271
1050 1050 a, g dbSNP:760783190
1053 1053 a, g dbSNP:756033789
1058 1058 c, t dbSNP:200472524
1059 1059 a, g dbSNP:376581340
1063 1063 g, t dbSNP:755479412
1064 1064 c, t dbSNP:779333951
1071 1071 a, c dbSNP:753187461
1072 1072 a, c, t dbSNP:369890550
1076 1076 c, t dbSNP:777969989
1077 1077 a, g dbSNP:377327853
1083 1083 a, t dbSNP:770441867
1086 1086 a, g, t dbSNP:780785303
1092 1092 c, g, t dbSNP:199724105
1093 1093 a, g dbSNP:376573652
1094 1094 c, t dbSNP:768542333
1098 1098 c, t dbSNP:762794979
1099 1099 a, g dbSNP:370858159
1102 1102 c, g dbSNP:766441670
1103 1103 c, t dbSNP:190756867
1104 1104 a, g dbSNP:759668482
1107 1107 a, c, g dbSNP:765295167
1108 1108 a, c dbSNP:376319970
1109 1109 c, t dbSNP:569717946
1110 1110 a, g, t dbSNP:183358379
1114 1114 c, g dbSNP:780655613
1121 1121 c, t dbSNP:374553856
1122 1122 c, t dbSNP:201943606
1123 1123 a, g dbSNP:199840628
1124 1124 c, g dbSNP:534563196
1141 1141 c, t dbSNP:201760569
1146 1146 a, g dbSNP:762354129
1150 1150 c, t dbSNP:372547107
1151 1151 a, g dbSNP:750775368
1157 1157 c, g dbSNP:760403544
1163 1163 c, g, t dbSNP:144028073
1164 1164 a, g dbSNP:754512829
1172 1172 c, t dbSNP:778453366
1173 1173 c, t dbSNP:752624557
1174 1174 a, t dbSNP:758283340
1176 1176 a, c, t dbSNP:369280939
1177 1177 a, g dbSNP:770017878
1179 1179 c, t dbSNP:780315495
1180 1180 a, g dbSNP:557621183
1193 1193 a, g dbSNP:768661795
1195 1195 a, t dbSNP:774470730
1198 1198 a, g dbSNP:373358594
1199 1199 c, t dbSNP:772636597
1201 1201 a, g dbSNP:773722982
1208 1208 c, t dbSNP:376127132
1209 1209 c, t dbSNP:139895699
1210 1210 a, g dbSNP:753470822
1212 1212 a, c, g dbSNP:759203655
1214 1214 a, g dbSNP:752211932
1215 1215 a, t dbSNP:758338480
1217 1217 a, c dbSNP:777631631
1223 1223 a, g dbSNP:560252337
1229 1229 a, g dbSNP:374471358
1230 1230 c, g dbSNP:781014025
1232 1232 c, g, t dbSNP:2032360
1233 1233 a, g dbSNP:376386930
1238 1238 a, g dbSNP:202026667
1241 1241 a, g dbSNP:772262266
1245 1245 c, g dbSNP:773921393
1253 1253 c, t dbSNP:747486507
1254 1254 a, c dbSNP:771471186
1259 1259 c, t dbSNP:763647926
1263 1263 a, t dbSNP:368204968
1264 1264 a, g, t dbSNP:372469887
1266 1266 c, t dbSNP:191545221
1269 1269 a, g dbSNP:756000963
1274 1274 c, t dbSNP:4761788
1276 1276 c, t dbSNP:201286632
1277 1277 a, g dbSNP:369312934
1280 1280 a, g dbSNP:778028956
1281 1281 a, g dbSNP:747018877
1283 1283 a, c, t dbSNP:28733260
1292 1292 g, t dbSNP:746358096
1296 1296 c, g, t dbSNP:770237949
1299 1299 c, g dbSNP:201484793
1306 1306 c, t dbSNP:534973333
1307 1307 a, g dbSNP:773861385
1309 1309 c, t dbSNP:202169108
1311 1311 c, g dbSNP:376312545
1316 1316 c, t dbSNP:11170086
1317 1317 a, g dbSNP:760506150
1321 1321 a, c dbSNP:766217821
1323 1323 c, t dbSNP:201833908
1324 1324 a, g dbSNP:373932045
1326 1326 c, t dbSNP:578093160
1332 1332 c, t dbSNP:11170087
1333 1333 c, t dbSNP:757425820
1337 1337 a, c, g, t dbSNP:376051219
1338 1338 a, g, t dbSNP:200353690
1341 1341 c, t dbSNP:749593452
1348 1348 g, t dbSNP:768991511
1349 1349 c, t dbSNP:11170088
1354 1354 c, t dbSNP:747720953
1360 1360 a, t dbSNP:771660500
1361 1361 g, t dbSNP:772546220
1375 1375 g, t dbSNP:760697549
1386 1386 a, c dbSNP:563550345
1389 1389 a, t dbSNP:776580728
1390 1390 a, g dbSNP:573751348
1394 1394 a, c, g dbSNP:375789021
1399 1399 a, g dbSNP:761921829
1408 1408 a, c, t dbSNP:767661038
1412 1412 c, t dbSNP:756653876
1417 1417 a, g dbSNP:542735645
1426 1426 c, t dbSNP:754335203
1433 1433 c, t dbSNP:755372953
1434 1434 a, c, g dbSNP:60687604
1439 1439 c, t dbSNP:747856782
1440 1440 a, g dbSNP:757946783
1442 1442 c, t dbSNP:777341221
1452 1452 c, t dbSNP:749369523
1453 1453 a, g dbSNP:199951808
1454 1454 c, t dbSNP:368480216
1458 1458 c, t dbSNP:370091437
1463 1463 a, g dbSNP:769763274
1465 1465 a, g dbSNP:775487582
1466 1466 a, c, t dbSNP:372174710
1467 1467 a, g dbSNP:121909129
1469 1469 g, t dbSNP:121909130
1490 1490 a, c dbSNP:749890373
1491 1491 a, g dbSNP:756138046
1494 1494 a, g dbSNP:779918036
1498 1498 a, c dbSNP:562353539
1499 1499 c, g dbSNP:768381688
1505 1505 c, t dbSNP:778718877
1509 1509 a, t dbSNP:747260525
1511 1511 a, c dbSNP:561311116
1512 1512 g, t dbSNP:763403025
1527 1527 a, g dbSNP:769143368
1532 1532 a, c, g dbSNP:527319461
1540 1540 c, g dbSNP:767957618
1552 1552 c, g dbSNP:773044010
1554 1554 a, g dbSNP:760323015
1555 1555 c, t dbSNP:765834889
1565 1565 a, t dbSNP:753350605
1571 1571 c, t dbSNP:754896113
1572 1572 a, c, g dbSNP:200098470
1582 1582 c, g dbSNP:758256483
1583 1583 c, t dbSNP:370419090
1584 1584 a, g dbSNP:746090200
1592 1592 c, t dbSNP:756291633
1596 1596 a, g dbSNP:780026281
1597 1597 a, t dbSNP:749337520
1600 1600 c, t dbSNP:768592222
1603 1603 a, g dbSNP:775033485
1604 1604 c, t dbSNP:748655954
1608 1608 a, g dbSNP:772581641
1616 1616 a, g dbSNP:773647506
1619 1619 c, g dbSNP:374122056
1623 1623 c, g dbSNP:560668616
1635 1635 a, g dbSNP:776126777
1638 1638 c, t dbSNP:532729838
1642 1642 c, t dbSNP:368279953
1645 1645 c, g dbSNP:752688759
1646 1646 a, c dbSNP:758272986
1648 1648 c, t dbSNP:763879273
1650 1650 g, t dbSNP:751326981
1654 1654 c, g, t dbSNP:756310592
1655 1655 g, t dbSNP:749435372
1657 1657 -, t dbSNP:752996118
1661 1661 a, c dbSNP:755171534
1662 1662 c, g, t dbSNP:779081505
1663 1663 c, t dbSNP:772669443
1668 1668 a, g dbSNP:773737109
1671 1671 a, c, g dbSNP:552762476
1678 1678 -, t dbSNP:756555632
1684 1684 a, g dbSNP:771169755
1687 1687 a, g dbSNP:201740931
1688 1688 c, t dbSNP:759097243
1689 1689 c, t dbSNP:769306504
1691 1691 a, g dbSNP:774913128
1694 1694 a, g dbSNP:148309398
1697 1697 g, t dbSNP:763968892
1701 1701 c, g dbSNP:751427480
1703 1703 a, c dbSNP:761704739
1707 1707 g, t dbSNP:548735427
1712 1712 a, c dbSNP:375430561
1713 1713 g, t dbSNP:755188054
1720 1720 a, g dbSNP:779167469
1722 1722 c, g dbSNP:752778941
1723 1723 c, g dbSNP:758531707
1733 1733 a, c dbSNP:568421074
1737 1737 a, c dbSNP:201257801
1750 1750 c, g dbSNP:771115534
1753 1753 c, t dbSNP:554027353
1785 1785 a, g dbSNP:2280949
1797 1797 c, g dbSNP:573713057
1815 1815 c, g dbSNP:559456695
1818 1818 c, t dbSNP:4076515
1852 1852 a, g dbSNP:768815810
1861 1861 -, g dbSNP:775440318
1900 1900 a, g dbSNP:185290279
1911 1911 a, g dbSNP:188381439
1918 1918 a, g dbSNP:548333894
1955 1955 a, g dbSNP:1137013
1962 1962 -, t, tt dbSNP:536030727
1963 1963 -, tt dbSNP:111847567
1970 1970 -, t, tt, ttt dbSNP:369883463
1971 1971 -, t dbSNP:3215437
1978 1978 a, g dbSNP:1137014
2003 2003 c, t dbSNP:1137015
2049 2049 a, g dbSNP:116249711
2108 2108 a, g dbSNP:532647627
2113 2113 c, t dbSNP:546535987
2133 2133 c, g dbSNP:563059388
2151 2151 a, g dbSNP:750820066
2161 2161 c, g dbSNP:141418442
2208 2208 a, c dbSNP:548699342
2224 2224 c, t dbSNP:767008402
2228 2228 a, g dbSNP:369975927
2263 2263 a, g dbSNP:527798405

Target ORF information:

RefSeq Version XM_011538338
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens keratin 86, type II (KRT86), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011538338

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACTTGTG GATCTTACTG TGGTGGCCGC GCCTTCAGCT GCATCTCGGC CTGCGGGCCC 
CGGCCCGGCC GCTGCTGCAT CACCGCCGCC CCCTACCGTG GCATCTCCTG CTACCGCGGC
CTCACCGGGG GCTTCGGCAG CCACAGCGTG TGCGGAGGCT TTCGGGCCGG CTCCTGCGGA
CGCAGCTTCG GCTACCGCTC CGGGGGCGTG TGCGGGCCCA GTCCCCCATG CATCACCACC
GTGTCGGTCA ACGAGAGCCT CCTCACGCCC CTCAACCTGG AGATCGACCC CAACGCGCAG
TGCGTGAAGC AGGAGGAGAA GGAGCAGATC AAGTCCCTCA ACAGCAGGTT CGCGGCCTTC
ATCGACAAGG TGCGCTTCCT GGAGCAGCAG AACAAACTGC TGGAGACAAA GCTGCAGTTC
TACCAGAACC GCGAGTGTTG CCAGAGCAAC CTGGAGCCCC TGTTTGAGGG CTACATCGAG
ACTCTGCGGC GGGAGGCCGA GTGCGTGGAG GCCGACAGCG GGAGGCTGGC CTCAGAGCTT
AACCACGTGC AGGAGGTGCT GGAGGGCTAC AAGAAGAAGT ATGAGGAGGA GGTTTCTCTG
AGAGCAACAG CTGAGAACGA GTTTGTGGCT CTGAAGAAGG ATGTGGACTG CGCCTACCTC
CGCAAATCAG ACCTGGAGGC CAATGTGGAG GCCCTGATCC AGGAGATCGA CTTCCTGAGG
CGGCTGTATG AGGAGGAGAT CCGCGTTCTC CAGTCCCACA TCTCAGACAC CTCCGTGGTT
GTCAAGCTGG ACAACAGCCG GGACCTGAAC ATGGACTGCA TCATTGCCGA GATCAAGGCA
CAGTACGATG ACATTGTCAC CCGTAGCCGG GCTGAGGCCG AGTCCTGGTA CCGCAGCAAG
TGTGAGGAGA TGAAGGCCAC GGTGATCAGG CACGGGGAGA CCCTGCGCCG CACCAAGGAG
GAGATCAACG AGCTGAACCG CATGATCCAG AGGCTGACGG CTGAGGTGGA GAATGCCAAG
TGCCAGAATT CCAAGCTGGA GGCTGCGGTG GCTCAGTCTG AGCAGCAGGG TGAGGCGGCC
CTCAGCGATG CCCGCTGCAA GTTGGCCGAG CTGGAGGGTG CCCTGCAGAA GGCCAAGCAG
GACATGGCCT GCCTGATCAG GGAGTACCAG GAGGTGATGA ACTCCAAGCT GGGCCTGGAC
ATCGAGATCG CCACCTACAG GCGCCTGCTG GAGGGCGAGG AGCAGAGGCT GTGCGAGGGC
GTCGGCTCGG TGAATGTCTG CGTCAGCAGC TCCCGCGGTG GCGTTGTCTG TGGCGATCTC
TGCGCCTCCA CTACTGCCCC TGTTGTCTCC ACCAGAGTCA GTAGCGTCCC CAGCAACAGC
AACGTGGTGG TGGGCACTAC TAACGCCTGC GCCCCCTCCG CCCGGGTTGG CGTCTGCGGC
GGCAGCTGTA AGAGGTGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17915
Accession Version NM_002284.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1461bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product keratin, type II cuticular Hb6
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Michael Rogers. The reference sequence was derived from AC121757.8 and BC069585.1. This sequence is a reference standard in the RefSeqGene project. On Jun 23, 2006 this sequence version replaced gi:15431325. Summary: The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this hair keratin, as well as KRTHB1 and KRTHB3, is found primarily in the hair cortex. Mutations in this gene and KRTHB1 have been observed in patients with a rare dominant hair disease, monilethrix. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X99142.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACTTGTG GATCTTACTG TGGTGGCCGC GCCTTCAGCT GCATCTCGGC CTGCGGGCCC 
CGGCCCGGCC GCTGCTGCAT CACCGCCGCC CCCTACCGTG GCATCTCCTG CTACCGCGGC
CTCACCGGGG GCTTCGGCAG CCACAGCGTG TGCGGAGGCT TTCGGGCCGG CTCCTGCGGA
CGCAGCTTCG GCTACCGCTC CGGGGGCGTG TGCGGGCCCA GTCCCCCATG CATCACCACC
GTGTCGGTCA ACGAGAGCCT CCTCACGCCC CTCAACCTGG AGATCGACCC CAACGCGCAG
TGCGTGAAGC AGGAGGAGAA GGAGCAGATC AAGTCCCTCA ACAGCAGGTT CGCGGCCTTC
ATCGACAAGG TGCGCTTCCT GGAGCAGCAG AACAAACTGC TGGAGACAAA GCTGCAGTTC
TACCAGAACC GCGAGTGTTG CCAGAGCAAC CTGGAGCCCC TGTTTGAGGG CTACATCGAG
ACTCTGCGGC GGGAGGCCGA GTGCGTGGAG GCCGACAGCG GGAGGCTGGC CTCAGAGCTT
AACCACGTGC AGGAGGTGCT GGAGGGCTAC AAGAAGAAGT ATGAGGAGGA GGTTTCTCTG
AGAGCAACAG CTGAGAACGA GTTTGTGGCT CTGAAGAAGG ATGTGGACTG CGCCTACCTC
CGCAAATCAG ACCTGGAGGC CAATGTGGAG GCCCTGATCC AGGAGATCGA CTTCCTGAGG
CGGCTGTATG AGGAGGAGAT CCGCGTTCTC CAGTCCCACA TCTCAGACAC CTCCGTGGTT
GTCAAGCTGG ACAACAGCCG GGACCTGAAC ATGGACTGCA TCATTGCCGA GATCAAGGCA
CAGTACGATG ACATTGTCAC CCGTAGCCGG GCTGAGGCCG AGTCCTGGTA CCGCAGCAAG
TGTGAGGAGA TGAAGGCCAC GGTGATCAGG CACGGGGAGA CCCTGCGCCG CACCAAGGAG
GAGATCAACG AGCTGAACCG CATGATCCAG AGGCTGACGG CTGAGGTGGA GAATGCCAAG
TGCCAGAATT CCAAGCTGGA GGCTGCGGTG GCTCAGTCTG AGCAGCAGGG TGAGGCGGCC
CTCAGCGATG CCCGCTGCAA GTTGGCCGAG CTGGAGGGTG CCCTGCAGAA GGCCAAGCAG
GACATGGCCT GCCTGATCAG GGAGTACCAG GAGGTGATGA ACTCCAAGCT GGGCCTGGAC
ATCGAGATCG CCACCTACAG GCGCCTGCTG GAGGGCGAGG AGCAGAGGCT GTGCGAGGGC
GTCGGCTCGG TGAATGTCTG CGTCAGCAGC TCCCGCGGTG GCGTTGTCTG TGGCGATCTC
TGCGCCTCCA CTACTGCCCC TGTTGTCTCC ACCAGAGTCA GTAGCGTCCC CAGCAACAGC
AACGTGGTGG TGGGCACTAC TAACGCCTGC GCCCCCTCCG CCCGGGTTGG CGTCTGCGGC
GGCAGCTGTA AGAGGTGCTA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_002275.1
CDS53..1513
Misc Feature(1)53..370(+)
Misc Feature(2)365..1300(+)
Misc Feature(3)371..1291(+)
Misc Feature(4)371..475(+)
Misc Feature(5)476..505(+)
Misc Feature(6)506..808(+)
Misc Feature(7)725..1072(+)
Misc Feature(8)809..859(+)
Misc Feature(9)860..1291(+)
Misc Feature(10)1292..1510(+)
Exon (1)1..421
Gene:KRT86
Gene Synonym:
Exon (2)422..630
Gene:KRT86
Gene Synonym:
Exon (3)631..691
Gene:KRT86
Gene Synonym:
Exon (4)692..787
Gene:KRT86
Gene Synonym:
Exon (5)788..952
Gene:KRT86
Gene Synonym:
Exon (6)953..1078
Gene:KRT86
Gene Synonym:
Exon (7)1079..1299
Gene:KRT86
Gene Synonym:
Exon (8)1300..1331
Gene:KRT86
Gene Synonym:
Exon (9)1332..2091
Gene:KRT86
Gene Synonym:
Translation
Position Chain Variation Link
7 7 a, c, t dbSNP:756381120
10 10 a, c dbSNP:749346976
12 12 a, g dbSNP:755444963
20 20 a, c, t dbSNP:56016927
21 21 c, t dbSNP:559412483
29 29 c, g dbSNP:773532478
32 32 c, g dbSNP:746715296
39 39 a, c dbSNP:770571275
43 43 a, c dbSNP:776039120
44 44 a, g dbSNP:528526293
48 48 a, g dbSNP:367636606
52 52 c, g dbSNP:775628307
56 56 a, t dbSNP:762980858
61 61 g, t dbSNP:764023107
62 62 a, g dbSNP:751348438
64 64 a, c dbSNP:756434438
66 66 a, c dbSNP:766555284
68 68 c, t dbSNP:117662922
73 73 g, t dbSNP:755139437
79 79 a, c, g, t dbSNP:201329364
80 80 a, c dbSNP:758877634
82 82 c, t dbSNP:368082897
84 84 a, c dbSNP:747337849
85 85 c, t dbSNP:371864554
89 89 a, g dbSNP:780844187
90 90 c, g dbSNP:565104845
95 95 a, g, t dbSNP:530949768
100 100 c, g dbSNP:199538989
104 104 g, t dbSNP:550585959
106 106 c, t dbSNP:74651927
107 107 a, g dbSNP:774291422
112 112 c, g, t dbSNP:201694571
114 114 a, c, g dbSNP:754153541
116 116 -, c dbSNP:745354445
118 118 c, g dbSNP:765348086
119 119 a, g, t dbSNP:752727601
122 122 a, c dbSNP:778190510
129 129 a, g dbSNP:751938228
130 130 c, t dbSNP:201420491
136 136 c, t dbSNP:781406879
138 138 c, t dbSNP:566680440
139 139 a, c, t dbSNP:112261068
144 144 c, g dbSNP:748739670
145 145 c, g dbSNP:768727542
149 149 c, t dbSNP:774487692
150 150 a, c, g, t dbSNP:552266351
151 151 c, t dbSNP:772858714
154 154 c, t dbSNP:759824354
157 157 c, g dbSNP:765405708
159 159 a, c dbSNP:202141424
160 160 c, t dbSNP:762965684
167 167 c, t dbSNP:764149570
168 168 a, g dbSNP:752176981
169 169 c, t dbSNP:757675096
175 175 a, c, t dbSNP:781658784
178 178 c, t dbSNP:755794129
179 179 c, g dbSNP:779794188
180 180 a, g dbSNP:748935718
181 181 c, g dbSNP:754598294
187 187 c, t dbSNP:778531514
189 189 c, g dbSNP:748162043
196 196 c, t dbSNP:771852441
200 200 a, g, t dbSNP:773093741
206 206 c, g dbSNP:376835872
207 207 a, g dbSNP:557368421
213 213 c, t dbSNP:574036785
215 215 c, t dbSNP:764200311
216 216 a, g dbSNP:774273613
220 220 c, t dbSNP:762407305
221 221 c, g, t dbSNP:369728157
224 224 g, t dbSNP:756604025
228 228 -, ctt dbSNP:775534639
229 229 c, t dbSNP:766845192
231 231 -, gacgca dbSNP:760642690
233 233 c, g dbSNP:753581743
241 241 c, t dbSNP:754651481
242 242 c, g dbSNP:778504570
244 244 a, c dbSNP:747568260
247 247 -, c dbSNP:768654938
248 248 a, c dbSNP:758364190
249 249 a, g dbSNP:57242951
253 253 c, t dbSNP:553105016
254 254 a, c, g dbSNP:573121340
256 256 c, g dbSNP:749564887
257 257 a, g dbSNP:768820064
274 274 c, t dbSNP:774523262
275 275 a, c dbSNP:761738178
276 276 c, t dbSNP:767618159
278 278 c, g dbSNP:773655908
284 284 a, g dbSNP:761233503
292 292 a, c dbSNP:766898287
298 298 a, g dbSNP:754205786
318 318 c, t dbSNP:754706890
327 327 a, g dbSNP:764732778
340 340 c, t dbSNP:545137193
346 346 c, t dbSNP:757904259
348 348 c, t dbSNP:777333199
349 349 a, g, t dbSNP:199751260
353 353 g, t dbSNP:780990129
356 356 a, g dbSNP:745624392
357 357 c, t dbSNP:768877244
386 386 -, ccctcaac dbSNP:776225547
392 392 a, c, g dbSNP:61091894
400 400 a, g dbSNP:58580222
404 404 a, g dbSNP:748394653
405 405 a, c dbSNP:60612575
449 449 a, c dbSNP:537434322
467 467 a, c dbSNP:771522388
468 468 a, c dbSNP:58717266
543 543 a, g dbSNP:548127873
557 557 g, t dbSNP:567721642
688 688 a, g dbSNP:79398941
693 693 a, g dbSNP:56677856
695 695 a, g dbSNP:781043236
701 701 a, t dbSNP:779212628
703 703 c, t dbSNP:377005978
713 713 c, t dbSNP:199678774
714 714 a, g, t dbSNP:531934335
717 717 a, t dbSNP:772288102
718 718 a, g dbSNP:201521867
725 725 a, c dbSNP:747016737
729 729 a, t dbSNP:771581226
731 731 a, g dbSNP:777336849
734 734 a, g dbSNP:760117096
736 736 c, t dbSNP:200750977
737 737 a, g dbSNP:775905498
744 744 a, c, t dbSNP:762539212
750 750 a, t dbSNP:751075241
756 756 a, g dbSNP:761368875
757 757 g, t dbSNP:200085173
760 760 c, t dbSNP:778154101
761 761 a, g dbSNP:756052236
771 771 a, g dbSNP:201433700
773 773 c, t dbSNP:111429470
774 774 a, g dbSNP:753627634
775 775 a, g dbSNP:758552461
778 778 a, g dbSNP:778081882
779 779 c, t dbSNP:759397233
780 780 a, c, g, t dbSNP:202150817
784 784 a, c, g dbSNP:78831098
785 785 a, g dbSNP:775752662
792 792 g, t dbSNP:776699267
794 794 c, t dbSNP:368604832
795 795 a, g, t dbSNP:765173749
796 796 c, t dbSNP:58523335
797 797 a, g dbSNP:61914259
804 804 a, g dbSNP:750485674
805 805 a, g dbSNP:756277678
807 807 c, t dbSNP:779962943
808 808 c, g, t dbSNP:61915660
816 816 c, t dbSNP:779276684
817 817 a, g dbSNP:748444485
818 818 g, t dbSNP:367712000
820 820 c, t dbSNP:371492790
823 823 c, g dbSNP:112956699
826 826 c, g dbSNP:770517793
827 827 a, g dbSNP:369679589
830 830 a, g, t dbSNP:759569087
851 851 c, t dbSNP:527924718
852 852 a, g dbSNP:371922719
864 864 c, g, t dbSNP:763786271
872 872 a, g dbSNP:760783190
875 875 a, g dbSNP:756033789
880 880 c, t dbSNP:200472524
881 881 a, g dbSNP:376581340
885 885 g, t dbSNP:755479412
886 886 c, t dbSNP:779333951
893 893 a, c dbSNP:753187461
894 894 a, c, t dbSNP:369890550
898 898 c, t dbSNP:777969989
899 899 a, g dbSNP:377327853
905 905 a, t dbSNP:770441867
908 908 a, g, t dbSNP:780785303
914 914 c, g, t dbSNP:199724105
915 915 a, g dbSNP:376573652
916 916 c, t dbSNP:768542333
920 920 c, t dbSNP:762794979
921 921 a, g dbSNP:370858159
924 924 c, g dbSNP:766441670
925 925 c, t dbSNP:190756867
926 926 a, g dbSNP:759668482
929 929 a, c, g dbSNP:765295167
930 930 a, c dbSNP:376319970
931 931 c, t dbSNP:569717946
932 932 a, g, t dbSNP:183358379
936 936 c, g dbSNP:780655613
943 943 c, t dbSNP:374553856
944 944 c, t dbSNP:201943606
945 945 a, g dbSNP:199840628
946 946 c, g dbSNP:534563196
963 963 c, t dbSNP:201760569
968 968 a, g dbSNP:762354129
972 972 c, t dbSNP:372547107
973 973 a, g dbSNP:750775368
979 979 c, g dbSNP:760403544
985 985 c, g, t dbSNP:144028073
986 986 a, g dbSNP:754512829
994 994 c, t dbSNP:778453366
995 995 c, t dbSNP:752624557
996 996 a, t dbSNP:758283340
998 998 a, c, t dbSNP:369280939
999 999 a, g dbSNP:770017878
1001 1001 c, t dbSNP:780315495
1002 1002 a, g dbSNP:557621183
1015 1015 a, g dbSNP:768661795
1017 1017 a, t dbSNP:774470730
1020 1020 a, g dbSNP:373358594
1021 1021 c, t dbSNP:772636597
1023 1023 a, g dbSNP:773722982
1030 1030 c, t dbSNP:376127132
1031 1031 c, t dbSNP:139895699
1032 1032 a, g dbSNP:753470822
1034 1034 a, c, g dbSNP:759203655
1036 1036 a, g dbSNP:752211932
1037 1037 a, t dbSNP:758338480
1039 1039 a, c dbSNP:777631631
1045 1045 a, g dbSNP:560252337
1051 1051 a, g dbSNP:374471358
1052 1052 c, g dbSNP:781014025
1054 1054 c, g, t dbSNP:2032360
1055 1055 a, g dbSNP:376386930
1060 1060 a, g dbSNP:202026667
1063 1063 a, g dbSNP:772262266
1067 1067 c, g dbSNP:773921393
1075 1075 c, t dbSNP:747486507
1076 1076 a, c dbSNP:771471186
1081 1081 c, t dbSNP:763647926
1085 1085 a, t dbSNP:368204968
1086 1086 a, g, t dbSNP:372469887
1088 1088 c, t dbSNP:191545221
1091 1091 a, g dbSNP:756000963
1096 1096 c, t dbSNP:4761788
1098 1098 c, t dbSNP:201286632
1099 1099 a, g dbSNP:369312934
1102 1102 a, g dbSNP:778028956
1103 1103 a, g dbSNP:747018877
1105 1105 a, c, t dbSNP:28733260
1114 1114 g, t dbSNP:746358096
1118 1118 c, g, t dbSNP:770237949
1121 1121 c, g dbSNP:201484793
1128 1128 c, t dbSNP:534973333
1129 1129 a, g dbSNP:773861385
1131 1131 c, t dbSNP:202169108
1133 1133 c, g dbSNP:376312545
1138 1138 c, t dbSNP:11170086
1139 1139 a, g dbSNP:760506150
1143 1143 a, c dbSNP:766217821
1145 1145 c, t dbSNP:201833908
1146 1146 a, g dbSNP:373932045
1148 1148 c, t dbSNP:578093160
1154 1154 c, t dbSNP:11170087
1155 1155 c, t dbSNP:757425820
1159 1159 a, c, g, t dbSNP:376051219
1160 1160 a, g, t dbSNP:200353690
1163 1163 c, t dbSNP:749593452
1170 1170 g, t dbSNP:768991511
1171 1171 c, t dbSNP:11170088
1176 1176 c, t dbSNP:747720953
1182 1182 a, t dbSNP:771660500
1183 1183 g, t dbSNP:772546220
1197 1197 g, t dbSNP:760697549
1208 1208 a, c dbSNP:563550345
1211 1211 a, t dbSNP:776580728
1212 1212 a, g dbSNP:573751348
1216 1216 a, c, g dbSNP:375789021
1221 1221 a, g dbSNP:761921829
1230 1230 a, c, t dbSNP:767661038
1234 1234 c, t dbSNP:756653876
1239 1239 a, g dbSNP:542735645
1248 1248 c, t dbSNP:754335203
1255 1255 c, t dbSNP:755372953
1256 1256 a, c, g dbSNP:60687604
1261 1261 c, t dbSNP:747856782
1262 1262 a, g dbSNP:757946783
1264 1264 c, t dbSNP:777341221
1274 1274 c, t dbSNP:749369523
1275 1275 a, g dbSNP:199951808
1276 1276 c, t dbSNP:368480216
1280 1280 c, t dbSNP:370091437
1285 1285 a, g dbSNP:769763274
1287 1287 a, g dbSNP:775487582
1288 1288 a, c, t dbSNP:372174710
1289 1289 a, g dbSNP:121909129
1291 1291 g, t dbSNP:121909130
1312 1312 a, c dbSNP:749890373
1313 1313 a, g dbSNP:756138046
1316 1316 a, g dbSNP:779918036
1320 1320 a, c dbSNP:562353539
1321 1321 c, g dbSNP:768381688
1327 1327 c, t dbSNP:778718877
1331 1331 a, t dbSNP:747260525
1333 1333 a, c dbSNP:561311116
1334 1334 g, t dbSNP:763403025
1349 1349 a, g dbSNP:769143368
1354 1354 a, c, g dbSNP:527319461
1362 1362 c, g dbSNP:767957618
1374 1374 c, g dbSNP:773044010
1376 1376 a, g dbSNP:760323015
1377 1377 c, t dbSNP:765834889
1387 1387 a, t dbSNP:753350605
1393 1393 c, t dbSNP:754896113
1394 1394 a, c, g dbSNP:200098470
1404 1404 c, g dbSNP:758256483
1405 1405 c, t dbSNP:370419090
1406 1406 a, g dbSNP:746090200
1414 1414 c, t dbSNP:756291633
1418 1418 a, g dbSNP:780026281
1419 1419 a, t dbSNP:749337520
1422 1422 c, t dbSNP:768592222
1425 1425 a, g dbSNP:775033485
1426 1426 c, t dbSNP:748655954
1430 1430 a, g dbSNP:772581641
1438 1438 a, g dbSNP:773647506
1441 1441 c, g dbSNP:374122056
1445 1445 c, g dbSNP:560668616
1457 1457 a, g dbSNP:776126777
1460 1460 c, t dbSNP:532729838
1464 1464 c, t dbSNP:368279953
1467 1467 c, g dbSNP:752688759
1468 1468 a, c dbSNP:758272986
1470 1470 c, t dbSNP:763879273
1472 1472 g, t dbSNP:751326981
1476 1476 c, g, t dbSNP:756310592
1477 1477 g, t dbSNP:749435372
1479 1479 -, t dbSNP:752996118
1483 1483 a, c dbSNP:755171534
1484 1484 c, g, t dbSNP:779081505
1485 1485 c, t dbSNP:772669443
1490 1490 a, g dbSNP:773737109
1493 1493 a, c, g dbSNP:552762476
1500 1500 -, t dbSNP:756555632
1506 1506 a, g dbSNP:771169755
1509 1509 a, g dbSNP:201740931
1510 1510 c, t dbSNP:759097243
1511 1511 c, t dbSNP:769306504
1513 1513 a, g dbSNP:774913128
1516 1516 a, g dbSNP:148309398
1519 1519 g, t dbSNP:763968892
1523 1523 c, g dbSNP:751427480
1525 1525 a, c dbSNP:761704739
1529 1529 g, t dbSNP:548735427
1534 1534 a, c dbSNP:375430561
1535 1535 g, t dbSNP:755188054
1542 1542 a, g dbSNP:779167469
1544 1544 c, g dbSNP:752778941
1545 1545 c, g dbSNP:758531707
1555 1555 a, c dbSNP:568421074
1559 1559 a, c dbSNP:201257801
1572 1572 c, g dbSNP:771115534
1575 1575 c, t dbSNP:554027353
1607 1607 a, g dbSNP:2280949
1619 1619 c, g dbSNP:573713057
1637 1637 c, g dbSNP:559456695
1640 1640 c, t dbSNP:4076515
1674 1674 a, g dbSNP:768815810
1683 1683 -, g dbSNP:775440318
1722 1722 a, g dbSNP:185290279
1733 1733 a, g dbSNP:188381439
1740 1740 a, g dbSNP:548333894
1777 1777 a, g dbSNP:1137013
1784 1784 -, t, tt dbSNP:536030727
1785 1785 -, tt dbSNP:111847567
1792 1792 -, t, tt, ttt dbSNP:369883463
1793 1793 -, t dbSNP:3215437
1800 1800 a, g dbSNP:1137014
1825 1825 c, t dbSNP:1137015
1871 1871 a, g dbSNP:116249711
1930 1930 a, g dbSNP:532647627
1935 1935 c, t dbSNP:546535987
1955 1955 c, g dbSNP:563059388
1973 1973 a, g dbSNP:750820066
1983 1983 c, g dbSNP:141418442
2030 2030 a, c dbSNP:548699342
2046 2046 c, t dbSNP:767008402
2050 2050 a, g dbSNP:369975927
2085 2085 a, g dbSNP:527798405

Target ORF information:

RefSeq Version NM_002284
Organism Homo sapiens (human)
Definition Homo sapiens keratin 86, type II (KRT86), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002284

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACTTGTG GATCTTACTG TGGTGGCCGC GCCTTCAGCT GCATCTCGGC CTGCGGGCCC 
CGGCCCGGCC GCTGCTGCAT CACCGCCGCC CCCTACCGTG GCATCTCCTG CTACCGCGGC
CTCACCGGGG GCTTCGGCAG CCACAGCGTG TGCGGAGGCT TTCGGGCCGG CTCCTGCGGA
CGCAGCTTCG GCTACCGCTC CGGGGGCGTG TGCGGGCCCA GTCCCCCATG CATCACCACC
GTGTCGGTCA ACGAGAGCCT CCTCACGCCC CTCAACCTGG AGATCGACCC CAACGCGCAG
TGCGTGAAGC AGGAGGAGAA GGAGCAGATC AAGTCCCTCA ACAGCAGGTT CGCGGCCTTC
ATCGACAAGG TGCGCTTCCT GGAGCAGCAG AACAAACTGC TGGAGACAAA GCTGCAGTTC
TACCAGAACC GCGAGTGTTG CCAGAGCAAC CTGGAGCCCC TGTTTGAGGG CTACATCGAG
ACTCTGCGGC GGGAGGCCGA GTGCGTGGAG GCCGACAGCG GGAGGCTGGC CTCAGAGCTT
AACCACGTGC AGGAGGTGCT GGAGGGCTAC AAGAAGAAGT ATGAGGAGGA GGTTTCTCTG
AGAGCAACAG CTGAGAACGA GTTTGTGGCT CTGAAGAAGG ATGTGGACTG CGCCTACCTC
CGCAAATCAG ACCTGGAGGC CAATGTGGAG GCCCTGATCC AGGAGATCGA CTTCCTGAGG
CGGCTGTATG AGGAGGAGAT CCGCGTTCTC CAGTCCCACA TCTCAGACAC CTCCGTGGTT
GTCAAGCTGG ACAACAGCCG GGACCTGAAC ATGGACTGCA TCATTGCCGA GATCAAGGCA
CAGTACGATG ACATTGTCAC CCGTAGCCGG GCTGAGGCCG AGTCCTGGTA CCGCAGCAAG
TGTGAGGAGA TGAAGGCCAC GGTGATCAGG CACGGGGAGA CCCTGCGCCG CACCAAGGAG
GAGATCAACG AGCTGAACCG CATGATCCAG AGGCTGACGG CTGAGGTGGA GAATGCCAAG
TGCCAGAATT CCAAGCTGGA GGCTGCGGTG GCTCAGTCTG AGCAGCAGGG TGAGGCGGCC
CTCAGCGATG CCCGCTGCAA GTTGGCCGAG CTGGAGGGTG CCCTGCAGAA GGCCAAGCAG
GACATGGCCT GCCTGATCAG GGAGTACCAG GAGGTGATGA ACTCCAAGCT GGGCCTGGAC
ATCGAGATCG CCACCTACAG GCGCCTGCTG GAGGGCGAGG AGCAGAGGCT GTGCGAGGGC
GTCGGCTCGG TGAATGTCTG CGTCAGCAGC TCCCGCGGTG GCGTTGTCTG TGGCGATCTC
TGCGCCTCCA CTACTGCCCC TGTTGTCTCC ACCAGAGTCA GTAGCGTCCC CAGCAACAGC
AACGTGGTGG TGGGCACTAC TAACGCCTGC GCCCCCTCCG CCCGGGTTGG CGTCTGCGGC
GGCAGCTGTA AGAGGTGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.