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MAFA cDNA ORF clone, Homo sapiens (human)

Gene Symbol MAFA
Entrez Gene ID 389692
Full Name v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A
Synonyms RIPE3b1, hMafA
General protein information
Preferred Names
transcription factor MafA
Names
transcription factor MafA
transcription factor RIPE3b1
pancreatic beta-cell-specific transcriptional activator
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

8

8q24.3

Summary MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).[supplied by OMIM, Mar 2008]. lac of sum
Disorder MIM:

610303


mRNA and Protein(s)

mRNA Protein Name
NM_201589 NP_963883 transcription factor MafA



Homo sapiens (human) MAFA NP_963883.2
Canis lupus familiaris (dog) MAFA XP_003431862.2
Bos taurus (cattle) MAFA XP_005215337.1
Mus musculus (house mouse) Mafa NP_919331.1
Rattus norvegicus (Norway rat) Mafa XP_006241965.1
Gallus gallus (chicken) MAFA NP_990356.1
Danio rerio (zebrafish) mafa NP_001076409.1
Xenopus (Silurana) tropicalis (western clawed frog) mafa NP_001027475.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MAFA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAFA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu23130 NM_201589 Homo sapiens v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A (MAFA), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu23130
Clone ID Related Accession (Same CDS sequence) NM_201589
Accession Version NM_201589.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1062bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 10-MAY-2014
Organism Homo sapiens (human)
Product transcription factor MafA
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC105118.23. On Dec 3, 2010 this sequence version replaced gi:71274110. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCCGCGG AGCTGGCGAT GGGCGCCGAG CTGCCCAGCA GCCCGCTGGC CATCGAGTAC 
GTCAACGACT TCGACCTGAT GAAGTTCGAG GTGAAGAAGG AGCCTCCCGA GGCCGAGCGC
TTCTGCCACC GCCTGCCGCC AGGCTCGCTG TCCTCGACGC CGCTCAGCAC GCCCTGCTCC
TCCGTGCCCT CCTCGCCCAG CTTCTGCGCG CCCAGCCCGG GCACCGGCGG CGGCGGCGGC
GCGGGGGGCG GCGGCGGCTC GTCTCAGGCC GGGGGCGCCC CCGGGCCGCC GAGCGGGGGC
CCCGGCGCCG TCGGGGGCAC CTCGGGGAAG CCGGCGCTGG AGGATCTGTA CTGGATGAGC
GGCTACCAGC ATCACCTCAA CCCCGAGGCG CTCAACCTGA CGCCCGAGGA CGCGGTGGAG
GCGCTCATCG GCAGCGGCCA CCACGGCGCG CACCACGGCG CGCACCACCC GGCGGCCGCC
GCAGCCTACG AGGCTTTCCG CGGCCCGGGC TTCGCGGGCG GCGGCGGAGC GGACGACATG
GGCGCCGGCC ACCACCACGG CGCGCACCAC GCCGCCCACC ATCACCACGC CGCCCACCAC
CACCACCACC ACCACCACCA CCATGGCGGC GCGGGACACG GCGGTGGCGC GGGCCACCAC
GTGCGCCTGG AGGAGCGCTT CTCCGACGAC CAGCTGGTGT CCATGTCGGT GCGCGAGCTG
AACCGGCAGC TCCGCGGCTT CAGCAAGGAG GAGGTCATCC GGCTCAAGCA GAAGCGGCGC
ACGCTCAAGA ACCGCGGCTA CGCGCAGTCC TGCCGCTTCA AGCGGGTGCA GCAGCGGCAC
ATTCTGGAGA GCGAGAAGTG CCAACTCCAG AGCCAGGTGG AGCAGCTGAA GCTGGAGGTG
GGGCGCCTGG CCAAAGAGCG GGACCTGTAC AAGGAGAAAT ACGAGAAGCT GGCGGGCCGG
GGCGGCCCCG GGAGCGCGGG CGGGGCCGGT TTCCCGCGGG AGCCTTCGCC GCCGCAGGCC
GGTCCCGGCG GGGCCAAGGG CACGGCCGAC TTCTTCCTGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_963883.2
CDS27..1088
Misc Feature(1)66..68(+)
Misc Feature(2)66..68(+)
Misc Feature(3)66..68(+)
Misc Feature(4)171..173(+)
Misc Feature(5)183..185(+)
Misc Feature(6)195..197(+)
Misc Feature(7)207..209(+)
Misc Feature(8)219..221(+)
Misc Feature(9)219..221(+)
Misc Feature(10)357..461(+)
Misc Feature(11)771..977(+)
Misc Feature(12)771..977(+)
Misc Feature(13)786..863(+)
Misc Feature(14)792..851(+)
Misc Feature(15)804..848(+)
Misc Feature(16)849..968(+)
Misc Feature(17)870..935(+)
Exon (1)1..2373
Gene:MAFA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_201589
Organism Homo sapiens (human)
Definition Homo sapiens v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A (MAFA), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_201589

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCCGCGG AGCTGGCGAT GGGCGCCGAG CTGCCCAGCA GCCCGCTGGC CATCGAGTAC 
GTCAACGACT TCGACCTGAT GAAGTTCGAG GTGAAGAAGG AGCCTCCCGA GGCCGAGCGC
TTCTGCCACC GCCTGCCGCC AGGCTCGCTG TCCTCGACGC CGCTCAGCAC GCCCTGCTCC
TCCGTGCCCT CCTCGCCCAG CTTCTGCGCG CCCAGCCCGG GCACCGGCGG CGGCGGCGGC
GCGGGGGGCG GCGGCGGCTC GTCTCAGGCC GGGGGCGCCC CCGGGCCGCC GAGCGGGGGC
CCCGGCGCCG TCGGGGGCAC CTCGGGGAAG CCGGCGCTGG AGGATCTGTA CTGGATGAGC
GGCTACCAGC ATCACCTCAA CCCCGAGGCG CTCAACCTGA CGCCCGAGGA CGCGGTGGAG
GCGCTCATCG GCAGCGGCCA CCACGGCGCG CACCACGGCG CGCACCACCC GGCGGCCGCC
GCAGCCTACG AGGCTTTCCG CGGCCCGGGC TTCGCGGGCG GCGGCGGAGC GGACGACATG
GGCGCCGGCC ACCACCACGG CGCGCACCAC GCCGCCCACC ATCACCACGC CGCCCACCAC
CACCACCACC ACCACCACCA CCATGGCGGC GCGGGACACG GCGGTGGCGC GGGCCACCAC
GTGCGCCTGG AGGAGCGCTT CTCCGACGAC CAGCTGGTGT CCATGTCGGT GCGCGAGCTG
AACCGGCAGC TCCGCGGCTT CAGCAAGGAG GAGGTCATCC GGCTCAAGCA GAAGCGGCGC
ACGCTCAAGA ACCGCGGCTA CGCGCAGTCC TGCCGCTTCA AGCGGGTGCA GCAGCGGCAC
ATTCTGGAGA GCGAGAAGTG CCAACTCCAG AGCCAGGTGG AGCAGCTGAA GCTGGAGGTG
GGGCGCCTGG CCAAAGAGCG GGACCTGTAC AAGGAGAAAT ACGAGAAGCT GGCGGGCCGG
GGCGGCCCCG GGAGCGCGGG CGGGGCCGGT TTCCCGCGGG AGCCTTCGCC GCCGCAGGCC
GGTCCCGGCG GGGCCAAGGG CACGGCCGAC TTCTTCCTGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Preventing p38 MAPK-mediated MafA degradation ameliorates beta-cell dysfunction under oxidative stress
Mol. Endocrinol. 27 (7), 1078-1090 (2013)
El Khattabi I and Sharma A.


book

Beta cell nuclear musculoaponeurotic fibrosarcoma oncogene family A (MafA) is deficient in type 2 diabetes
Diabetologia 55 (11), 2985-2988 (2012)
Butler AE, Robertson RP, Hernandez R, Matveyenko AV, Gurlo T and Butler PC.


book

Combined transfection of the three transcriptional factors, PDX-1, NeuroD1, and MafA, causes differentiation of bone marrow mesenchymal stem cells into insulin-producing cells
Exp Diabetes Res 2012, 672013 (2012)
Guo QS, Zhu MY, Wang L, Fan XJ, Lu YH, Wang ZW, Zhu SJ, Wang Y and Huang Y.


book

ATF2 interacts with beta-cell-enriched transcription factors, MafA, Pdx1, and beta2, and activates insulin gene transcription
J. Biol. Chem. 286 (12), 10449-10456 (2011)
Han SI, Yasuda K and Kataoka K.


book

Regulation of beta-cell-specific and glucose-dependent MafA expression
Islets 3 (1), 35-37 (2011)
Vanderford NL.


book

A crucial role of MafA as a novel therapeutic target for diabetes
J. Biol. Chem. 280 (15), 15047-15052 (2005)
Kaneto H, Matsuoka TA, Nakatani Y, Miyatsuka T, Matsuhisa M, Hori M and Yamasaki Y.


book

The MafA transcription factor appears to be responsible for tissue-specific expression of insulin
Proc. Natl. Acad. Sci. U.S.A. 101 (9), 2930-2933 (2004)
Matsuoka TA, Artner I, Henderson E, Means A, Sander M and Stein R.


book

MafA is a glucose-regulated and pancreatic beta-cell-specific transcriptional activator for the insulin gene
J. Biol. Chem. 277 (51), 49903-49910 (2002)
Kataoka K, Han SI, Shioda S, Hirai M, Nishizawa M and Handa H.


book

Identification of beta-cell-specific insulin gene transcription factor RIPE3b1 as mammalian MafA
Proc. Natl. Acad. Sci. U.S.A. 99 (10), 6737-6742 (2002)
Olbrot M, Rud J, Moss LG and Sharma A.


book

Phosphorylation of MafA is essential for its transcriptional and biological properties
Mol. Cell. Biol. 21 (14), 4441-4452 (2001)
Benkhelifa S, Provot S, Nabais E, Eychene A, Calothy G and Felder-Schmittbuhl MP.


 
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