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LAMB3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol LAMB3
Entrez Gene ID 3914
Full Name laminin, beta 3
Synonyms AI1A, BM600-125KDA, LAM5, LAMNB1
General protein information
Preferred Names
laminin subunit beta-3
Names
laminin subunit beta-3
nicein-125kDa
kalinin-140kDa
kalinin B1 chain
laminin B1k chain
laminin S B3 chain
nicein subunit beta
kalinin subunit beta
epiligrin subunit bata
laminin-5 subunit beta
laminin, beta 3 (nicein (125kD), kalinin (140kD), BM600 (125kD))
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q32

Summary The product encoded by this gene is a laminin that belongs to a family of basement membrane proteins. This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. Mutations in this gene cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

150310

Disorder Html: Epidermolysis bullosa, junctional, Herlitz type, 226700 (3);

mRNA and Protein(s)

mRNA Protein Name
XM_005273124 XP_005273181 laminin subunit beta-3 isoform X1
NM_001127641 NP_001121113 laminin subunit beta-3 precursor
NM_000228 NP_000219 laminin subunit beta-3 precursor
NM_001017402 NP_001017402 laminin subunit beta-3 precursor



Homo sapiens (human) LAMB3 NP_001017402.1
Pan troglodytes (chimpanzee) LAMB3 XP_003949737.1
Macaca mulatta (Rhesus monkey) LAMB3 XP_002802197.1
Canis lupus familiaris (dog) LAMB3 XP_005622385.1
Bos taurus (cattle) LAMB3 NP_001075065.1
Mus musculus (house mouse) Lamb3 NP_001264857.1
Rattus norvegicus (Norway rat) Lamb3 NP_001094311.1
Gallus gallus (chicken) LAMB3 XP_425827.3
Xenopus (Silurana) tropicalis (western clawed frog) lamb3 XP_002933550.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LAMB3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LAMB3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu17378 XM_005273124 PREDICTED: Homo sapiens laminin, beta 3 (LAMB3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $384.30
$549.00
OHu17378 NM_001127641 Homo sapiens laminin, beta 3 (LAMB3), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $384.30
$549.00
OHu17378 NM_000228 Homo sapiens laminin, beta 3 (LAMB3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $384.30
$549.00
OHu17378 NM_001017402 Homo sapiens laminin, beta 3 (LAMB3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $384.30
$549.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu17378
Clone ID Related Accession (Same CDS sequence) NM_000228 , NM_001017402 , NM_001127641 , XM_005273124
Accession Version XM_005273124.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product laminin subunit beta-3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578800921. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGACCAT TCTTCCTCTT GTGTTTTGCC CTGCCTGGCC TCCTGCATGC CCAACAAGCC 
TGCTCCCGTG GGGCCTGCTA TCCACCTGTT GGGGACCTGC TTGTTGGGAG GACCCGGTTT
CTCCGAGCTT CATCTACCTG TGGACTGACC AAGCCTGAGA CCTACTGCAC CCAGTATGGC
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGCAGC CTCACAACTA CTACAGTCAC
CGAGTAGAGA ATGTGGCTTC ATCCTCCGGC CCCATGCGCT GGTGGCAGTC ACAGAATGAT
GTGAACCCTG TCTCTCTGCA GCTGGACCTG GACAGGAGAT TCCAGCTTCA AGAAGTCATG
ATGGAGTTCC AGGGGCCCAT GCCCGCCGGC ATGCTGATTG AGCGCTCCTC AGACTTCGGT
AAGACCTGGC GAGTGTACCA GTACCTGGCT GCCGACTGCA CCTCCACCTT CCCTCGGGTC
CGCCAGGGTC GGCCTCAGAG CTGGCAGGAT GTTCGGTGCC AGTCCCTGCC TCAGAGGCCT
AATGCACGCC TAAATGGGGG GAAGGTCCAA CTTAACCTTA TGGATTTAGT GTCTGGGATT
CCAGCAACTC AAAGTCAAAA AATTCAAGAG GTGGGGGAGA TCACAAACTT GAGAGTCAAT
TTCACCAGGC TGGCCCCTGT GCCCCAAAGG GGCTACCACC CTCCCAGCGC CTACTATGCT
GTGTCCCAGC TCCGTCTGCA GGGGAGCTGC TTCTGTCACG GCCATGCTGA TCGCTGCGCA
CCCAAGCCTG GGGCCTCTGC AGGCCCCTCC ACCGCTGTGC AGGTCCACGA TGTCTGTGTC
TGCCAGCACA ACACTGCCGG CCCAAATTGT GAGCGCTGTG CACCCTTCTA CAACAACCGG
CCCTGGAGAC CGGCGGAGGG CCAGGACGCC CATGAATGCC AAAGGTGCGA CTGCAATGGG
CACTCAGAGA CATGTCACTT TGACCCCGCT GTGTTTGCCG CCAGCCAGGG GGCATATGGA
GGTGTGTGTG ACAATTGCCG GGACCACACC GAAGGCAAGA ACTGTGAGCG GTGTCAGCTG
CACTATTTCC GGAACCGGCG CCCGGGAGCT TCCATTCAGG AGACCTGCAT CTCCTGCGAG
TGTGATCCGG ATGGGGCAGT GCCAGGGGCT CCCTGTGACC CAGTGACCGG GCAGTGTGTG
TGCAAGGAGC ATGTGCAGGG AGAGCGCTGT GACCTATGCA AGCCGGGCTT CACTGGACTC
ACCTACGCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA ACATCCTGGG GTCCCGGAGG
GACATGCCGT GTGACGAGGA GAGTGGGCGC TGCCTTTGTC TGCCCAACGT GGTGGGTCCC
AAATGTGACC AGTGTGCTCC CTACCACTGG AAGCTGGCCA GTGGCCAGGG CTGTGAACCG
TGTGCCTGCG ACCCGCACAA CTCCCTCAGC CCACAGTGCA ACCAGTTCAC AGGGCAGTGC
CCCTGTCGGG AAGGCTTTGG TGGCCTGATG TGCAGCGCTG CAGCCATCCG CCAGTGTCCA
GACCGGACCT ATGGAGACGT GGCCACAGGA TGCCGAGCCT GTGACTGTGA TTTCCGGGGA
ACAGAGGGCC CGGGCTGCGA CAAGGCATCA GGCCGCTGCC TCTGCCGCCC TGGCTTGACC
GGGCCCCGCT GTGACCAGTG CCAGCGAGGC TACTGTAATC GCTACCCGGT GTGCGTGGCC
TGCCACCCTT GCTTCCAGAC CTATGATGCG GACCTCCGGG AGCAGGCCCT GCGCTTTGGT
AGACTCCGCA ATGCCACCGC CAGCCTGTGG TCAGGGCCTG GGCTGGAGGA CCGTGGCCTG
GCCTCCCGGA TCCTAGATGC AAAGAGTAAG ATTGAGCAGA TCCGAGCAGT TCTCAGCAGC
CCCGCAGTCA CAGAGCAGGA GGTGGCTCAG GTGGCCAGTG CCATCCTCTC CCTCAGGCGA
ACTCTCCAGG GCCTGCAGCT GGATCTGCCC CTGGAGGAGG AGACGTTGTC CCTTCCGAGA
GACCTGGAGA GTCTTGACAG AAGCTTCAAT GGTCTCCTTA CTATGTATCA GAGGAAGAGG
GAGCAGTTTG AAAAAATAAG CAGTGCTGAT CCTTCAGGAG CCTTCCGGAT GCTGAGCACA
GCCTACGAGC AGTCAGCCCA GGCTGCTCAG CAGGTCTCCG ACAGCTCGCG CCTTTTGGAC
CAGCTCAGGG ACAGCCGGAG AGAGGCAGAG AGGCTGGTGC GGCAGGCGGG AGGAGGAGGA
GGCACCGGCA GCCCCAAGCT TGTGGCCCTG AGGCTGGAGA TGTCTTCGTT GCCTGACCTG
ACACCCACCT TCAACAAGCT CTGTGGCAAC TCCAGGCAGA TGGCTTGCAC CCCAATATCA
TGCCCTGGTG AGCTATGTCC CCAAGACAAT GGCACAGCCT GTGGCTCCCG CTGCAGGGGT
GTCCTTCCCA GGGCCGGTGG GGCCTTCTTG ATGGCGGGGC AGGTGGCTGA GCAGCTGCGG
GGCTTCAATG CCCAGCTCCA GCGGACCAGG CAGATGATTA GGGCAGCCGA GGAATCTGCC
TCACAGATTC AATCCAGTGC CCAGCGCTTG GAGACCCAGG TGAGCGCCAG CCGCTCCCAG
ATGGAGGAAG ATGTCAGACG CACACGGCTC CTAATCCAGC AGGTCCGGGA CTTCCTAACA
GACCCCGACA CTGATGCAGC CACTATCCAG GAGGTCAGCG AGGCCGTGCT GGCCCTGTGG
CTGCCCACAG ACTCAGCTAC TGTTCTGCAG AAGATGAATG AGATCCAGGC CATTGCAGCC
AGGCTCCCCA ACGTGGACTT GGTGCTGTCC CAGACCAAGC AGGACATTGC GCGTGCCCGC
CGGTTGCAGG CTGAGGCTGA GGAAGCCAGG AGCCGAGCCC ATGCAGTGGA GGGCCAGGTG
GAAGATGTGG TTGGGAACCT GCGGCAGGGG ACAGTGGCAC TGCAGGAAGC TCAGGACACC
ATGCAAGGCA CCAGCCGCTC CCTTCGGCTT ATCCAGGACA GGGTTGCTGA GGTTCAGCAG
GTACTGCGGC CAGCAGAAAA GCTGGTGACA AGCATGACCA AGCAGCTGGG TGACTTCTGG
ACACGGATGG AGGAGCTCCG CCACCAAGCC CGGCAGCAGG GGGCAGAGGC AGTCCAGGCC
CAGCAGCTTG CGGAAGGTGC CAGCGAGCAG GCATTGAGTG CCCAAGAGGG ATTTGAGAGA
ATAAAACAAA AGTATGCTGA GTTGAAGGAC CGGTTGGGTC AGAGTTCCAT GCTGGGTGAG
CAGGGTGCCC GGATCCAGAG TGTGAAGACA GAGGCAGAGG AGCTGTTTGG GGAGACCATG
GAGATGATGG ACAGGATGAA AGACATGGAG TTGGAGCTGC TGCGGGGCAG CCAGGCCATC
ATGCTGCGCT CAGCGGACCT GACAGGACTG GAGAAGCGTG TGGAGCAGAT CCGTGACCAC
ATCAATGGGC GCGTGCTCTA CTATGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005273181.1
CDS323..3841
Misc Feature(1)380..1066(+)
Misc Feature(2)1067..1234(+)
Misc Feature(3)1070..1192(+)
Misc Feature(4)1265..1429(+)
Misc Feature(5)1268..1387(+)
Misc Feature(6)1454..1609(+)
Misc Feature(7)1457..1552(+)
Misc Feature(8)1610..1759(+)
Misc Feature(9)1613..1708(+)
Misc Feature(10)1760..1918(+)
Misc Feature(11)1763..1855(+)
Misc Feature(12)1919..2068(+)
Misc Feature(13)1922..2014(+)
Misc Feature(14)2852..3535(+)
Misc Feature(15)2906..3550(+)
Misc Feature(16)2939..3550(+)
Misc Feature(17)3197..3313(+)
Translation

Target ORF information:

RefSeq Version XM_005273124
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens laminin, beta 3 (LAMB3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005273124

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGACCAT TCTTCCTCTT GTGTTTTGCC CTGCCTGGCC TCCTGCATGC CCAACAAGCC 
TGCTCCCGTG GGGCCTGCTA TCCACCTGTT GGGGACCTGC TTGTTGGGAG GACCCGGTTT
CTCCGAGCTT CATCTACCTG TGGACTGACC AAGCCTGAGA CCTACTGCAC CCAGTATGGC
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGCAGC CTCACAACTA CTACAGTCAC
CGAGTAGAGA ATGTGGCTTC ATCCTCCGGC CCCATGCGCT GGTGGCAGTC ACAGAATGAT
GTGAACCCTG TCTCTCTGCA GCTGGACCTG GACAGGAGAT TCCAGCTTCA AGAAGTCATG
ATGGAGTTCC AGGGGCCCAT GCCCGCCGGC ATGCTGATTG AGCGCTCCTC AGACTTCGGT
AAGACCTGGC GAGTGTACCA GTACCTGGCT GCCGACTGCA CCTCCACCTT CCCTCGGGTC
CGCCAGGGTC GGCCTCAGAG CTGGCAGGAT GTTCGGTGCC AGTCCCTGCC TCAGAGGCCT
AATGCACGCC TAAATGGGGG GAAGGTCCAA CTTAACCTTA TGGATTTAGT GTCTGGGATT
CCAGCAACTC AAAGTCAAAA AATTCAAGAG GTGGGGGAGA TCACAAACTT GAGAGTCAAT
TTCACCAGGC TGGCCCCTGT GCCCCAAAGG GGCTACCACC CTCCCAGCGC CTACTATGCT
GTGTCCCAGC TCCGTCTGCA GGGGAGCTGC TTCTGTCACG GCCATGCTGA TCGCTGCGCA
CCCAAGCCTG GGGCCTCTGC AGGCCCCTCC ACCGCTGTGC AGGTCCACGA TGTCTGTGTC
TGCCAGCACA ACACTGCCGG CCCAAATTGT GAGCGCTGTG CACCCTTCTA CAACAACCGG
CCCTGGAGAC CGGCGGAGGG CCAGGACGCC CATGAATGCC AAAGGTGCGA CTGCAATGGG
CACTCAGAGA CATGTCACTT TGACCCCGCT GTGTTTGCCG CCAGCCAGGG GGCATATGGA
GGTGTGTGTG ACAATTGCCG GGACCACACC GAAGGCAAGA ACTGTGAGCG GTGTCAGCTG
CACTATTTCC GGAACCGGCG CCCGGGAGCT TCCATTCAGG AGACCTGCAT CTCCTGCGAG
TGTGATCCGG ATGGGGCAGT GCCAGGGGCT CCCTGTGACC CAGTGACCGG GCAGTGTGTG
TGCAAGGAGC ATGTGCAGGG AGAGCGCTGT GACCTATGCA AGCCGGGCTT CACTGGACTC
ACCTACGCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA ACATCCTGGG GTCCCGGAGG
GACATGCCGT GTGACGAGGA GAGTGGGCGC TGCCTTTGTC TGCCCAACGT GGTGGGTCCC
AAATGTGACC AGTGTGCTCC CTACCACTGG AAGCTGGCCA GTGGCCAGGG CTGTGAACCG
TGTGCCTGCG ACCCGCACAA CTCCCTCAGC CCACAGTGCA ACCAGTTCAC AGGGCAGTGC
CCCTGTCGGG AAGGCTTTGG TGGCCTGATG TGCAGCGCTG CAGCCATCCG CCAGTGTCCA
GACCGGACCT ATGGAGACGT GGCCACAGGA TGCCGAGCCT GTGACTGTGA TTTCCGGGGA
ACAGAGGGCC CGGGCTGCGA CAAGGCATCA GGCCGCTGCC TCTGCCGCCC TGGCTTGACC
GGGCCCCGCT GTGACCAGTG CCAGCGAGGC TACTGTAATC GCTACCCGGT GTGCGTGGCC
TGCCACCCTT GCTTCCAGAC CTATGATGCG GACCTCCGGG AGCAGGCCCT GCGCTTTGGT
AGACTCCGCA ATGCCACCGC CAGCCTGTGG TCAGGGCCTG GGCTGGAGGA CCGTGGCCTG
GCCTCCCGGA TCCTAGATGC AAAGAGTAAG ATTGAGCAGA TCCGAGCAGT TCTCAGCAGC
CCCGCAGTCA CAGAGCAGGA GGTGGCTCAG GTGGCCAGTG CCATCCTCTC CCTCAGGCGA
ACTCTCCAGG GCCTGCAGCT GGATCTGCCC CTGGAGGAGG AGACGTTGTC CCTTCCGAGA
GACCTGGAGA GTCTTGACAG AAGCTTCAAT GGTCTCCTTA CTATGTATCA GAGGAAGAGG
GAGCAGTTTG AAAAAATAAG CAGTGCTGAT CCTTCAGGAG CCTTCCGGAT GCTGAGCACA
GCCTACGAGC AGTCAGCCCA GGCTGCTCAG CAGGTCTCCG ACAGCTCGCG CCTTTTGGAC
CAGCTCAGGG ACAGCCGGAG AGAGGCAGAG AGGCTGGTGC GGCAGGCGGG AGGAGGAGGA
GGCACCGGCA GCCCCAAGCT TGTGGCCCTG AGGCTGGAGA TGTCTTCGTT GCCTGACCTG
ACACCCACCT TCAACAAGCT CTGTGGCAAC TCCAGGCAGA TGGCTTGCAC CCCAATATCA
TGCCCTGGTG AGCTATGTCC CCAAGACAAT GGCACAGCCT GTGGCTCCCG CTGCAGGGGT
GTCCTTCCCA GGGCCGGTGG GGCCTTCTTG ATGGCGGGGC AGGTGGCTGA GCAGCTGCGG
GGCTTCAATG CCCAGCTCCA GCGGACCAGG CAGATGATTA GGGCAGCCGA GGAATCTGCC
TCACAGATTC AATCCAGTGC CCAGCGCTTG GAGACCCAGG TGAGCGCCAG CCGCTCCCAG
ATGGAGGAAG ATGTCAGACG CACACGGCTC CTAATCCAGC AGGTCCGGGA CTTCCTAACA
GACCCCGACA CTGATGCAGC CACTATCCAG GAGGTCAGCG AGGCCGTGCT GGCCCTGTGG
CTGCCCACAG ACTCAGCTAC TGTTCTGCAG AAGATGAATG AGATCCAGGC CATTGCAGCC
AGGCTCCCCA ACGTGGACTT GGTGCTGTCC CAGACCAAGC AGGACATTGC GCGTGCCCGC
CGGTTGCAGG CTGAGGCTGA GGAAGCCAGG AGCCGAGCCC ATGCAGTGGA GGGCCAGGTG
GAAGATGTGG TTGGGAACCT GCGGCAGGGG ACAGTGGCAC TGCAGGAAGC TCAGGACACC
ATGCAAGGCA CCAGCCGCTC CCTTCGGCTT ATCCAGGACA GGGTTGCTGA GGTTCAGCAG
GTACTGCGGC CAGCAGAAAA GCTGGTGACA AGCATGACCA AGCAGCTGGG TGACTTCTGG
ACACGGATGG AGGAGCTCCG CCACCAAGCC CGGCAGCAGG GGGCAGAGGC AGTCCAGGCC
CAGCAGCTTG CGGAAGGTGC CAGCGAGCAG GCATTGAGTG CCCAAGAGGG ATTTGAGAGA
ATAAAACAAA AGTATGCTGA GTTGAAGGAC CGGTTGGGTC AGAGTTCCAT GCTGGGTGAG
CAGGGTGCCC GGATCCAGAG TGTGAAGACA GAGGCAGAGG AGCTGTTTGG GGAGACCATG
GAGATGATGG ACAGGATGAA AGACATGGAG TTGGAGCTGC TGCGGGGCAG CCAGGCCATC
ATGCTGCGCT CAGCGGACCT GACAGGACTG GAGAAGCGTG TGGAGCAGAT CCGTGACCAC
ATCAATGGGC GCGTGCTCTA CTATGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17378
Clone ID Related Accession (Same CDS sequence) NM_000228 , NM_001017402 , NM_001127641 , XM_005273124
Accession Version NM_001127641.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product laminin subunit beta-3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL031316.2, BC075838.1, BP350980.1 and AY035783.1. Transcript Variant: This variant (3, also known as B3B) has an alternate 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L25541.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGACCAT TCTTCCTCTT GTGTTTTGCC CTGCCTGGCC TCCTGCATGC CCAACAAGCC 
TGCTCCCGTG GGGCCTGCTA TCCACCTGTT GGGGACCTGC TTGTTGGGAG GACCCGGTTT
CTCCGAGCTT CATCTACCTG TGGACTGACC AAGCCTGAGA CCTACTGCAC CCAGTATGGC
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGCAGC CTCACAACTA CTACAGTCAC
CGAGTAGAGA ATGTGGCTTC ATCCTCCGGC CCCATGCGCT GGTGGCAGTC ACAGAATGAT
GTGAACCCTG TCTCTCTGCA GCTGGACCTG GACAGGAGAT TCCAGCTTCA AGAAGTCATG
ATGGAGTTCC AGGGGCCCAT GCCCGCCGGC ATGCTGATTG AGCGCTCCTC AGACTTCGGT
AAGACCTGGC GAGTGTACCA GTACCTGGCT GCCGACTGCA CCTCCACCTT CCCTCGGGTC
CGCCAGGGTC GGCCTCAGAG CTGGCAGGAT GTTCGGTGCC AGTCCCTGCC TCAGAGGCCT
AATGCACGCC TAAATGGGGG GAAGGTCCAA CTTAACCTTA TGGATTTAGT GTCTGGGATT
CCAGCAACTC AAAGTCAAAA AATTCAAGAG GTGGGGGAGA TCACAAACTT GAGAGTCAAT
TTCACCAGGC TGGCCCCTGT GCCCCAAAGG GGCTACCACC CTCCCAGCGC CTACTATGCT
GTGTCCCAGC TCCGTCTGCA GGGGAGCTGC TTCTGTCACG GCCATGCTGA TCGCTGCGCA
CCCAAGCCTG GGGCCTCTGC AGGCCCCTCC ACCGCTGTGC AGGTCCACGA TGTCTGTGTC
TGCCAGCACA ACACTGCCGG CCCAAATTGT GAGCGCTGTG CACCCTTCTA CAACAACCGG
CCCTGGAGAC CGGCGGAGGG CCAGGACGCC CATGAATGCC AAAGGTGCGA CTGCAATGGG
CACTCAGAGA CATGTCACTT TGACCCCGCT GTGTTTGCCG CCAGCCAGGG GGCATATGGA
GGTGTGTGTG ACAATTGCCG GGACCACACC GAAGGCAAGA ACTGTGAGCG GTGTCAGCTG
CACTATTTCC GGAACCGGCG CCCGGGAGCT TCCATTCAGG AGACCTGCAT CTCCTGCGAG
TGTGATCCGG ATGGGGCAGT GCCAGGGGCT CCCTGTGACC CAGTGACCGG GCAGTGTGTG
TGCAAGGAGC ATGTGCAGGG AGAGCGCTGT GACCTATGCA AGCCGGGCTT CACTGGACTC
ACCTACGCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA ACATCCTGGG GTCCCGGAGG
GACATGCCGT GTGACGAGGA GAGTGGGCGC TGCCTTTGTC TGCCCAACGT GGTGGGTCCC
AAATGTGACC AGTGTGCTCC CTACCACTGG AAGCTGGCCA GTGGCCAGGG CTGTGAACCG
TGTGCCTGCG ACCCGCACAA CTCCCTCAGC CCACAGTGCA ACCAGTTCAC AGGGCAGTGC
CCCTGTCGGG AAGGCTTTGG TGGCCTGATG TGCAGCGCTG CAGCCATCCG CCAGTGTCCA
GACCGGACCT ATGGAGACGT GGCCACAGGA TGCCGAGCCT GTGACTGTGA TTTCCGGGGA
ACAGAGGGCC CGGGCTGCGA CAAGGCATCA GGCCGCTGCC TCTGCCGCCC TGGCTTGACC
GGGCCCCGCT GTGACCAGTG CCAGCGAGGC TACTGTAATC GCTACCCGGT GTGCGTGGCC
TGCCACCCTT GCTTCCAGAC CTATGATGCG GACCTCCGGG AGCAGGCCCT GCGCTTTGGT
AGACTCCGCA ATGCCACCGC CAGCCTGTGG TCAGGGCCTG GGCTGGAGGA CCGTGGCCTG
GCCTCCCGGA TCCTAGATGC AAAGAGTAAG ATTGAGCAGA TCCGAGCAGT TCTCAGCAGC
CCCGCAGTCA CAGAGCAGGA GGTGGCTCAG GTGGCCAGTG CCATCCTCTC CCTCAGGCGA
ACTCTCCAGG GCCTGCAGCT GGATCTGCCC CTGGAGGAGG AGACGTTGTC CCTTCCGAGA
GACCTGGAGA GTCTTGACAG AAGCTTCAAT GGTCTCCTTA CTATGTATCA GAGGAAGAGG
GAGCAGTTTG AAAAAATAAG CAGTGCTGAT CCTTCAGGAG CCTTCCGGAT GCTGAGCACA
GCCTACGAGC AGTCAGCCCA GGCTGCTCAG CAGGTCTCCG ACAGCTCGCG CCTTTTGGAC
CAGCTCAGGG ACAGCCGGAG AGAGGCAGAG AGGCTGGTGC GGCAGGCGGG AGGAGGAGGA
GGCACCGGCA GCCCCAAGCT TGTGGCCCTG AGGCTGGAGA TGTCTTCGTT GCCTGACCTG
ACACCCACCT TCAACAAGCT CTGTGGCAAC TCCAGGCAGA TGGCTTGCAC CCCAATATCA
TGCCCTGGTG AGCTATGTCC CCAAGACAAT GGCACAGCCT GTGGCTCCCG CTGCAGGGGT
GTCCTTCCCA GGGCCGGTGG GGCCTTCTTG ATGGCGGGGC AGGTGGCTGA GCAGCTGCGG
GGCTTCAATG CCCAGCTCCA GCGGACCAGG CAGATGATTA GGGCAGCCGA GGAATCTGCC
TCACAGATTC AATCCAGTGC CCAGCGCTTG GAGACCCAGG TGAGCGCCAG CCGCTCCCAG
ATGGAGGAAG ATGTCAGACG CACACGGCTC CTAATCCAGC AGGTCCGGGA CTTCCTAACA
GACCCCGACA CTGATGCAGC CACTATCCAG GAGGTCAGCG AGGCCGTGCT GGCCCTGTGG
CTGCCCACAG ACTCAGCTAC TGTTCTGCAG AAGATGAATG AGATCCAGGC CATTGCAGCC
AGGCTCCCCA ACGTGGACTT GGTGCTGTCC CAGACCAAGC AGGACATTGC GCGTGCCCGC
CGGTTGCAGG CTGAGGCTGA GGAAGCCAGG AGCCGAGCCC ATGCAGTGGA GGGCCAGGTG
GAAGATGTGG TTGGGAACCT GCGGCAGGGG ACAGTGGCAC TGCAGGAAGC TCAGGACACC
ATGCAAGGCA CCAGCCGCTC CCTTCGGCTT ATCCAGGACA GGGTTGCTGA GGTTCAGCAG
GTACTGCGGC CAGCAGAAAA GCTGGTGACA AGCATGACCA AGCAGCTGGG TGACTTCTGG
ACACGGATGG AGGAGCTCCG CCACCAAGCC CGGCAGCAGG GGGCAGAGGC AGTCCAGGCC
CAGCAGCTTG CGGAAGGTGC CAGCGAGCAG GCATTGAGTG CCCAAGAGGG ATTTGAGAGA
ATAAAACAAA AGTATGCTGA GTTGAAGGAC CGGTTGGGTC AGAGTTCCAT GCTGGGTGAG
CAGGGTGCCC GGATCCAGAG TGTGAAGACA GAGGCAGAGG AGCTGTTTGG GGAGACCATG
GAGATGATGG ACAGGATGAA AGACATGGAG TTGGAGCTGC TGCGGGGCAG CCAGGCCATC
ATGCTGCGCT CAGCGGACCT GACAGGACTG GAGAAGCGTG TGGAGCAGAT CCGTGACCAC
ATCAATGGGC GCGTGCTCTA CTATGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001121113.1
CDS119..3637
Misc Feature(1)50..52(+)
Misc Feature(2)176..862(+)
Misc Feature(3)863..1030(+)
Misc Feature(4)866..988(+)
Misc Feature(5)1061..1225(+)
Misc Feature(6)1064..1183(+)
Misc Feature(7)1250..1405(+)
Misc Feature(8)1253..1348(+)
Misc Feature(9)1406..1555(+)
Misc Feature(10)1409..1504(+)
Misc Feature(11)1556..1714(+)
Misc Feature(12)1559..1651(+)
Misc Feature(13)1715..1864(+)
Misc Feature(14)1718..1810(+)
Misc Feature(15)1853..2473(+)
Misc Feature(16)2474..2566(+)
Misc Feature(17)2567..3628(+)
Misc Feature(18)2648..3331(+)
Misc Feature(19)2702..3346(+)
Misc Feature(20)2735..3346(+)
Misc Feature(21)2993..3109(+)
Exon (1)1..81
Gene:LAMB3
Gene Synonym:
Exon (2)82..146
Gene:LAMB3
Gene Synonym:
Exon (3)147..301
Gene:LAMB3
Gene Synonym:
Exon (4)302..416
Gene:LAMB3
Gene Synonym:
Exon (5)417..490
Gene:LAMB3
Gene Synonym:
Exon (6)491..682
Gene:LAMB3
Gene Synonym:
Exon (7)683..746
Gene:LAMB3
Gene Synonym:
Exon (8)747..940
Gene:LAMB3
Gene Synonym:
Exon (9)941..1061
Gene:LAMB3
Gene Synonym:
Exon (10)1062..1250
Gene:LAMB3
Gene Synonym:
Exon (11)1251..1406
Gene:LAMB3
Gene Synonym:
Exon (12)1407..1603
Gene:LAMB3
Gene Synonym:
Exon (13)1604..1715
Gene:LAMB3
Gene Synonym:
Exon (14)1716..2094
Gene:LAMB3
Gene Synonym:
Exon (15)2095..2255
Gene:LAMB3
Gene Synonym:
Exon (16)2256..2476
Gene:LAMB3
Gene Synonym:
Exon (17)2477..2674
Gene:LAMB3
Gene Synonym:
Exon (18)2675..2819
Gene:LAMB3
Gene Synonym:
Exon (19)2820..3027
Gene:LAMB3
Gene Synonym:
Exon (20)3028..3169
Gene:LAMB3
Gene Synonym:
Exon (21)3170..3346
Gene:LAMB3
Gene Synonym:
Exon (22)3347..3500
Gene:LAMB3
Gene Synonym:
Exon (23)3501..4038
Gene:LAMB3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001127641
Organism Homo sapiens (human)
Definition Homo sapiens laminin, beta 3 (LAMB3), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001127641

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGACCAT TCTTCCTCTT GTGTTTTGCC CTGCCTGGCC TCCTGCATGC CCAACAAGCC 
TGCTCCCGTG GGGCCTGCTA TCCACCTGTT GGGGACCTGC TTGTTGGGAG GACCCGGTTT
CTCCGAGCTT CATCTACCTG TGGACTGACC AAGCCTGAGA CCTACTGCAC CCAGTATGGC
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGCAGC CTCACAACTA CTACAGTCAC
CGAGTAGAGA ATGTGGCTTC ATCCTCCGGC CCCATGCGCT GGTGGCAGTC ACAGAATGAT
GTGAACCCTG TCTCTCTGCA GCTGGACCTG GACAGGAGAT TCCAGCTTCA AGAAGTCATG
ATGGAGTTCC AGGGGCCCAT GCCCGCCGGC ATGCTGATTG AGCGCTCCTC AGACTTCGGT
AAGACCTGGC GAGTGTACCA GTACCTGGCT GCCGACTGCA CCTCCACCTT CCCTCGGGTC
CGCCAGGGTC GGCCTCAGAG CTGGCAGGAT GTTCGGTGCC AGTCCCTGCC TCAGAGGCCT
AATGCACGCC TAAATGGGGG GAAGGTCCAA CTTAACCTTA TGGATTTAGT GTCTGGGATT
CCAGCAACTC AAAGTCAAAA AATTCAAGAG GTGGGGGAGA TCACAAACTT GAGAGTCAAT
TTCACCAGGC TGGCCCCTGT GCCCCAAAGG GGCTACCACC CTCCCAGCGC CTACTATGCT
GTGTCCCAGC TCCGTCTGCA GGGGAGCTGC TTCTGTCACG GCCATGCTGA TCGCTGCGCA
CCCAAGCCTG GGGCCTCTGC AGGCCCCTCC ACCGCTGTGC AGGTCCACGA TGTCTGTGTC
TGCCAGCACA ACACTGCCGG CCCAAATTGT GAGCGCTGTG CACCCTTCTA CAACAACCGG
CCCTGGAGAC CGGCGGAGGG CCAGGACGCC CATGAATGCC AAAGGTGCGA CTGCAATGGG
CACTCAGAGA CATGTCACTT TGACCCCGCT GTGTTTGCCG CCAGCCAGGG GGCATATGGA
GGTGTGTGTG ACAATTGCCG GGACCACACC GAAGGCAAGA ACTGTGAGCG GTGTCAGCTG
CACTATTTCC GGAACCGGCG CCCGGGAGCT TCCATTCAGG AGACCTGCAT CTCCTGCGAG
TGTGATCCGG ATGGGGCAGT GCCAGGGGCT CCCTGTGACC CAGTGACCGG GCAGTGTGTG
TGCAAGGAGC ATGTGCAGGG AGAGCGCTGT GACCTATGCA AGCCGGGCTT CACTGGACTC
ACCTACGCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA ACATCCTGGG GTCCCGGAGG
GACATGCCGT GTGACGAGGA GAGTGGGCGC TGCCTTTGTC TGCCCAACGT GGTGGGTCCC
AAATGTGACC AGTGTGCTCC CTACCACTGG AAGCTGGCCA GTGGCCAGGG CTGTGAACCG
TGTGCCTGCG ACCCGCACAA CTCCCTCAGC CCACAGTGCA ACCAGTTCAC AGGGCAGTGC
CCCTGTCGGG AAGGCTTTGG TGGCCTGATG TGCAGCGCTG CAGCCATCCG CCAGTGTCCA
GACCGGACCT ATGGAGACGT GGCCACAGGA TGCCGAGCCT GTGACTGTGA TTTCCGGGGA
ACAGAGGGCC CGGGCTGCGA CAAGGCATCA GGCCGCTGCC TCTGCCGCCC TGGCTTGACC
GGGCCCCGCT GTGACCAGTG CCAGCGAGGC TACTGTAATC GCTACCCGGT GTGCGTGGCC
TGCCACCCTT GCTTCCAGAC CTATGATGCG GACCTCCGGG AGCAGGCCCT GCGCTTTGGT
AGACTCCGCA ATGCCACCGC CAGCCTGTGG TCAGGGCCTG GGCTGGAGGA CCGTGGCCTG
GCCTCCCGGA TCCTAGATGC AAAGAGTAAG ATTGAGCAGA TCCGAGCAGT TCTCAGCAGC
CCCGCAGTCA CAGAGCAGGA GGTGGCTCAG GTGGCCAGTG CCATCCTCTC CCTCAGGCGA
ACTCTCCAGG GCCTGCAGCT GGATCTGCCC CTGGAGGAGG AGACGTTGTC CCTTCCGAGA
GACCTGGAGA GTCTTGACAG AAGCTTCAAT GGTCTCCTTA CTATGTATCA GAGGAAGAGG
GAGCAGTTTG AAAAAATAAG CAGTGCTGAT CCTTCAGGAG CCTTCCGGAT GCTGAGCACA
GCCTACGAGC AGTCAGCCCA GGCTGCTCAG CAGGTCTCCG ACAGCTCGCG CCTTTTGGAC
CAGCTCAGGG ACAGCCGGAG AGAGGCAGAG AGGCTGGTGC GGCAGGCGGG AGGAGGAGGA
GGCACCGGCA GCCCCAAGCT TGTGGCCCTG AGGCTGGAGA TGTCTTCGTT GCCTGACCTG
ACACCCACCT TCAACAAGCT CTGTGGCAAC TCCAGGCAGA TGGCTTGCAC CCCAATATCA
TGCCCTGGTG AGCTATGTCC CCAAGACAAT GGCACAGCCT GTGGCTCCCG CTGCAGGGGT
GTCCTTCCCA GGGCCGGTGG GGCCTTCTTG ATGGCGGGGC AGGTGGCTGA GCAGCTGCGG
GGCTTCAATG CCCAGCTCCA GCGGACCAGG CAGATGATTA GGGCAGCCGA GGAATCTGCC
TCACAGATTC AATCCAGTGC CCAGCGCTTG GAGACCCAGG TGAGCGCCAG CCGCTCCCAG
ATGGAGGAAG ATGTCAGACG CACACGGCTC CTAATCCAGC AGGTCCGGGA CTTCCTAACA
GACCCCGACA CTGATGCAGC CACTATCCAG GAGGTCAGCG AGGCCGTGCT GGCCCTGTGG
CTGCCCACAG ACTCAGCTAC TGTTCTGCAG AAGATGAATG AGATCCAGGC CATTGCAGCC
AGGCTCCCCA ACGTGGACTT GGTGCTGTCC CAGACCAAGC AGGACATTGC GCGTGCCCGC
CGGTTGCAGG CTGAGGCTGA GGAAGCCAGG AGCCGAGCCC ATGCAGTGGA GGGCCAGGTG
GAAGATGTGG TTGGGAACCT GCGGCAGGGG ACAGTGGCAC TGCAGGAAGC TCAGGACACC
ATGCAAGGCA CCAGCCGCTC CCTTCGGCTT ATCCAGGACA GGGTTGCTGA GGTTCAGCAG
GTACTGCGGC CAGCAGAAAA GCTGGTGACA AGCATGACCA AGCAGCTGGG TGACTTCTGG
ACACGGATGG AGGAGCTCCG CCACCAAGCC CGGCAGCAGG GGGCAGAGGC AGTCCAGGCC
CAGCAGCTTG CGGAAGGTGC CAGCGAGCAG GCATTGAGTG CCCAAGAGGG ATTTGAGAGA
ATAAAACAAA AGTATGCTGA GTTGAAGGAC CGGTTGGGTC AGAGTTCCAT GCTGGGTGAG
CAGGGTGCCC GGATCCAGAG TGTGAAGACA GAGGCAGAGG AGCTGTTTGG GGAGACCATG
GAGATGATGG ACAGGATGAA AGACATGGAG TTGGAGCTGC TGCGGGGCAG CCAGGCCATC
ATGCTGCGCT CAGCGGACCT GACAGGACTG GAGAAGCGTG TGGAGCAGAT CCGTGACCAC
ATCAATGGGC GCGTGCTCTA CTATGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17378
Clone ID Related Accession (Same CDS sequence) NM_000228 , NM_001017402 , NM_001127641 , XM_005273124
Accession Version NM_000228.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product laminin subunit beta-3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL555685.3, BC058922.1, AY035783.1 and BC075838.1. This sequence is a reference standard in the RefSeqGene project. On Apr 23, 2005 this sequence version replaced gi:4557712. Transcript Variant: This variant (1, also known as B3A) encodes the same protein as variants 2 and 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058922.1, D37766.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGACCAT TCTTCCTCTT GTGTTTTGCC CTGCCTGGCC TCCTGCATGC CCAACAAGCC 
TGCTCCCGTG GGGCCTGCTA TCCACCTGTT GGGGACCTGC TTGTTGGGAG GACCCGGTTT
CTCCGAGCTT CATCTACCTG TGGACTGACC AAGCCTGAGA CCTACTGCAC CCAGTATGGC
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGCAGC CTCACAACTA CTACAGTCAC
CGAGTAGAGA ATGTGGCTTC ATCCTCCGGC CCCATGCGCT GGTGGCAGTC ACAGAATGAT
GTGAACCCTG TCTCTCTGCA GCTGGACCTG GACAGGAGAT TCCAGCTTCA AGAAGTCATG
ATGGAGTTCC AGGGGCCCAT GCCCGCCGGC ATGCTGATTG AGCGCTCCTC AGACTTCGGT
AAGACCTGGC GAGTGTACCA GTACCTGGCT GCCGACTGCA CCTCCACCTT CCCTCGGGTC
CGCCAGGGTC GGCCTCAGAG CTGGCAGGAT GTTCGGTGCC AGTCCCTGCC TCAGAGGCCT
AATGCACGCC TAAATGGGGG GAAGGTCCAA CTTAACCTTA TGGATTTAGT GTCTGGGATT
CCAGCAACTC AAAGTCAAAA AATTCAAGAG GTGGGGGAGA TCACAAACTT GAGAGTCAAT
TTCACCAGGC TGGCCCCTGT GCCCCAAAGG GGCTACCACC CTCCCAGCGC CTACTATGCT
GTGTCCCAGC TCCGTCTGCA GGGGAGCTGC TTCTGTCACG GCCATGCTGA TCGCTGCGCA
CCCAAGCCTG GGGCCTCTGC AGGCCCCTCC ACCGCTGTGC AGGTCCACGA TGTCTGTGTC
TGCCAGCACA ACACTGCCGG CCCAAATTGT GAGCGCTGTG CACCCTTCTA CAACAACCGG
CCCTGGAGAC CGGCGGAGGG CCAGGACGCC CATGAATGCC AAAGGTGCGA CTGCAATGGG
CACTCAGAGA CATGTCACTT TGACCCCGCT GTGTTTGCCG CCAGCCAGGG GGCATATGGA
GGTGTGTGTG ACAATTGCCG GGACCACACC GAAGGCAAGA ACTGTGAGCG GTGTCAGCTG
CACTATTTCC GGAACCGGCG CCCGGGAGCT TCCATTCAGG AGACCTGCAT CTCCTGCGAG
TGTGATCCGG ATGGGGCAGT GCCAGGGGCT CCCTGTGACC CAGTGACCGG GCAGTGTGTG
TGCAAGGAGC ATGTGCAGGG AGAGCGCTGT GACCTATGCA AGCCGGGCTT CACTGGACTC
ACCTACGCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA ACATCCTGGG GTCCCGGAGG
GACATGCCGT GTGACGAGGA GAGTGGGCGC TGCCTTTGTC TGCCCAACGT GGTGGGTCCC
AAATGTGACC AGTGTGCTCC CTACCACTGG AAGCTGGCCA GTGGCCAGGG CTGTGAACCG
TGTGCCTGCG ACCCGCACAA CTCCCTCAGC CCACAGTGCA ACCAGTTCAC AGGGCAGTGC
CCCTGTCGGG AAGGCTTTGG TGGCCTGATG TGCAGCGCTG CAGCCATCCG CCAGTGTCCA
GACCGGACCT ATGGAGACGT GGCCACAGGA TGCCGAGCCT GTGACTGTGA TTTCCGGGGA
ACAGAGGGCC CGGGCTGCGA CAAGGCATCA GGCCGCTGCC TCTGCCGCCC TGGCTTGACC
GGGCCCCGCT GTGACCAGTG CCAGCGAGGC TACTGTAATC GCTACCCGGT GTGCGTGGCC
TGCCACCCTT GCTTCCAGAC CTATGATGCG GACCTCCGGG AGCAGGCCCT GCGCTTTGGT
AGACTCCGCA ATGCCACCGC CAGCCTGTGG TCAGGGCCTG GGCTGGAGGA CCGTGGCCTG
GCCTCCCGGA TCCTAGATGC AAAGAGTAAG ATTGAGCAGA TCCGAGCAGT TCTCAGCAGC
CCCGCAGTCA CAGAGCAGGA GGTGGCTCAG GTGGCCAGTG CCATCCTCTC CCTCAGGCGA
ACTCTCCAGG GCCTGCAGCT GGATCTGCCC CTGGAGGAGG AGACGTTGTC CCTTCCGAGA
GACCTGGAGA GTCTTGACAG AAGCTTCAAT GGTCTCCTTA CTATGTATCA GAGGAAGAGG
GAGCAGTTTG AAAAAATAAG CAGTGCTGAT CCTTCAGGAG CCTTCCGGAT GCTGAGCACA
GCCTACGAGC AGTCAGCCCA GGCTGCTCAG CAGGTCTCCG ACAGCTCGCG CCTTTTGGAC
CAGCTCAGGG ACAGCCGGAG AGAGGCAGAG AGGCTGGTGC GGCAGGCGGG AGGAGGAGGA
GGCACCGGCA GCCCCAAGCT TGTGGCCCTG AGGCTGGAGA TGTCTTCGTT GCCTGACCTG
ACACCCACCT TCAACAAGCT CTGTGGCAAC TCCAGGCAGA TGGCTTGCAC CCCAATATCA
TGCCCTGGTG AGCTATGTCC CCAAGACAAT GGCACAGCCT GTGGCTCCCG CTGCAGGGGT
GTCCTTCCCA GGGCCGGTGG GGCCTTCTTG ATGGCGGGGC AGGTGGCTGA GCAGCTGCGG
GGCTTCAATG CCCAGCTCCA GCGGACCAGG CAGATGATTA GGGCAGCCGA GGAATCTGCC
TCACAGATTC AATCCAGTGC CCAGCGCTTG GAGACCCAGG TGAGCGCCAG CCGCTCCCAG
ATGGAGGAAG ATGTCAGACG CACACGGCTC CTAATCCAGC AGGTCCGGGA CTTCCTAACA
GACCCCGACA CTGATGCAGC CACTATCCAG GAGGTCAGCG AGGCCGTGCT GGCCCTGTGG
CTGCCCACAG ACTCAGCTAC TGTTCTGCAG AAGATGAATG AGATCCAGGC CATTGCAGCC
AGGCTCCCCA ACGTGGACTT GGTGCTGTCC CAGACCAAGC AGGACATTGC GCGTGCCCGC
CGGTTGCAGG CTGAGGCTGA GGAAGCCAGG AGCCGAGCCC ATGCAGTGGA GGGCCAGGTG
GAAGATGTGG TTGGGAACCT GCGGCAGGGG ACAGTGGCAC TGCAGGAAGC TCAGGACACC
ATGCAAGGCA CCAGCCGCTC CCTTCGGCTT ATCCAGGACA GGGTTGCTGA GGTTCAGCAG
GTACTGCGGC CAGCAGAAAA GCTGGTGACA AGCATGACCA AGCAGCTGGG TGACTTCTGG
ACACGGATGG AGGAGCTCCG CCACCAAGCC CGGCAGCAGG GGGCAGAGGC AGTCCAGGCC
CAGCAGCTTG CGGAAGGTGC CAGCGAGCAG GCATTGAGTG CCCAAGAGGG ATTTGAGAGA
ATAAAACAAA AGTATGCTGA GTTGAAGGAC CGGTTGGGTC AGAGTTCCAT GCTGGGTGAG
CAGGGTGCCC GGATCCAGAG TGTGAAGACA GAGGCAGAGG AGCTGTTTGG GGAGACCATG
GAGATGATGG ACAGGATGAA AGACATGGAG TTGGAGCTGC TGCGGGGCAG CCAGGCCATC
ATGCTGCGCT CAGCGGACCT GACAGGACTG GAGAAGCGTG TGGAGCAGAT CCGTGACCAC
ATCAATGGGC GCGTGCTCTA CTATGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000219.2
CDS145..3663
Misc Feature(1)7..9(+)
Misc Feature(2)202..888(+)
Misc Feature(3)889..1056(+)
Misc Feature(4)892..1014(+)
Misc Feature(5)1087..1251(+)
Misc Feature(6)1090..1209(+)
Misc Feature(7)1276..1431(+)
Misc Feature(8)1279..1374(+)
Misc Feature(9)1432..1581(+)
Misc Feature(10)1435..1530(+)
Misc Feature(11)1582..1740(+)
Misc Feature(12)1585..1677(+)
Misc Feature(13)1741..1890(+)
Misc Feature(14)1744..1836(+)
Misc Feature(15)1879..2499(+)
Misc Feature(16)2500..2592(+)
Misc Feature(17)2593..3654(+)
Misc Feature(18)2674..3357(+)
Misc Feature(19)2728..3372(+)
Misc Feature(20)2761..3372(+)
Misc Feature(21)3019..3135(+)
Exon (1)1..107
Gene:LAMB3
Gene Synonym:
Exon (2)108..172
Gene:LAMB3
Gene Synonym:
Exon (3)173..327
Gene:LAMB3
Gene Synonym:
Exon (4)328..442
Gene:LAMB3
Gene Synonym:
Exon (5)443..516
Gene:LAMB3
Gene Synonym:
Exon (6)517..708
Gene:LAMB3
Gene Synonym:
Exon (7)709..772
Gene:LAMB3
Gene Synonym:
Exon (8)773..966
Gene:LAMB3
Gene Synonym:
Exon (9)967..1087
Gene:LAMB3
Gene Synonym:
Exon (10)1088..1276
Gene:LAMB3
Gene Synonym:
Exon (11)1277..1432
Gene:LAMB3
Gene Synonym:
Exon (12)1433..1629
Gene:LAMB3
Gene Synonym:
Exon (13)1630..1741
Gene:LAMB3
Gene Synonym:
Exon (14)1742..2120
Gene:LAMB3
Gene Synonym:
Exon (15)2121..2281
Gene:LAMB3
Gene Synonym:
Exon (16)2282..2502
Gene:LAMB3
Gene Synonym:
Exon (17)2503..2700
Gene:LAMB3
Gene Synonym:
Exon (18)2701..2845
Gene:LAMB3
Gene Synonym:
Exon (19)2846..3053
Gene:LAMB3
Gene Synonym:
Exon (20)3054..3195
Gene:LAMB3
Gene Synonym:
Exon (21)3196..3372
Gene:LAMB3
Gene Synonym:
Exon (22)3373..3526
Gene:LAMB3
Gene Synonym:
Exon (23)3527..4064
Gene:LAMB3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000228
Organism Homo sapiens (human)
Definition Homo sapiens laminin, beta 3 (LAMB3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000228

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGACCAT TCTTCCTCTT GTGTTTTGCC CTGCCTGGCC TCCTGCATGC CCAACAAGCC 
TGCTCCCGTG GGGCCTGCTA TCCACCTGTT GGGGACCTGC TTGTTGGGAG GACCCGGTTT
CTCCGAGCTT CATCTACCTG TGGACTGACC AAGCCTGAGA CCTACTGCAC CCAGTATGGC
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGCAGC CTCACAACTA CTACAGTCAC
CGAGTAGAGA ATGTGGCTTC ATCCTCCGGC CCCATGCGCT GGTGGCAGTC ACAGAATGAT
GTGAACCCTG TCTCTCTGCA GCTGGACCTG GACAGGAGAT TCCAGCTTCA AGAAGTCATG
ATGGAGTTCC AGGGGCCCAT GCCCGCCGGC ATGCTGATTG AGCGCTCCTC AGACTTCGGT
AAGACCTGGC GAGTGTACCA GTACCTGGCT GCCGACTGCA CCTCCACCTT CCCTCGGGTC
CGCCAGGGTC GGCCTCAGAG CTGGCAGGAT GTTCGGTGCC AGTCCCTGCC TCAGAGGCCT
AATGCACGCC TAAATGGGGG GAAGGTCCAA CTTAACCTTA TGGATTTAGT GTCTGGGATT
CCAGCAACTC AAAGTCAAAA AATTCAAGAG GTGGGGGAGA TCACAAACTT GAGAGTCAAT
TTCACCAGGC TGGCCCCTGT GCCCCAAAGG GGCTACCACC CTCCCAGCGC CTACTATGCT
GTGTCCCAGC TCCGTCTGCA GGGGAGCTGC TTCTGTCACG GCCATGCTGA TCGCTGCGCA
CCCAAGCCTG GGGCCTCTGC AGGCCCCTCC ACCGCTGTGC AGGTCCACGA TGTCTGTGTC
TGCCAGCACA ACACTGCCGG CCCAAATTGT GAGCGCTGTG CACCCTTCTA CAACAACCGG
CCCTGGAGAC CGGCGGAGGG CCAGGACGCC CATGAATGCC AAAGGTGCGA CTGCAATGGG
CACTCAGAGA CATGTCACTT TGACCCCGCT GTGTTTGCCG CCAGCCAGGG GGCATATGGA
GGTGTGTGTG ACAATTGCCG GGACCACACC GAAGGCAAGA ACTGTGAGCG GTGTCAGCTG
CACTATTTCC GGAACCGGCG CCCGGGAGCT TCCATTCAGG AGACCTGCAT CTCCTGCGAG
TGTGATCCGG ATGGGGCAGT GCCAGGGGCT CCCTGTGACC CAGTGACCGG GCAGTGTGTG
TGCAAGGAGC ATGTGCAGGG AGAGCGCTGT GACCTATGCA AGCCGGGCTT CACTGGACTC
ACCTACGCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA ACATCCTGGG GTCCCGGAGG
GACATGCCGT GTGACGAGGA GAGTGGGCGC TGCCTTTGTC TGCCCAACGT GGTGGGTCCC
AAATGTGACC AGTGTGCTCC CTACCACTGG AAGCTGGCCA GTGGCCAGGG CTGTGAACCG
TGTGCCTGCG ACCCGCACAA CTCCCTCAGC CCACAGTGCA ACCAGTTCAC AGGGCAGTGC
CCCTGTCGGG AAGGCTTTGG TGGCCTGATG TGCAGCGCTG CAGCCATCCG CCAGTGTCCA
GACCGGACCT ATGGAGACGT GGCCACAGGA TGCCGAGCCT GTGACTGTGA TTTCCGGGGA
ACAGAGGGCC CGGGCTGCGA CAAGGCATCA GGCCGCTGCC TCTGCCGCCC TGGCTTGACC
GGGCCCCGCT GTGACCAGTG CCAGCGAGGC TACTGTAATC GCTACCCGGT GTGCGTGGCC
TGCCACCCTT GCTTCCAGAC CTATGATGCG GACCTCCGGG AGCAGGCCCT GCGCTTTGGT
AGACTCCGCA ATGCCACCGC CAGCCTGTGG TCAGGGCCTG GGCTGGAGGA CCGTGGCCTG
GCCTCCCGGA TCCTAGATGC AAAGAGTAAG ATTGAGCAGA TCCGAGCAGT TCTCAGCAGC
CCCGCAGTCA CAGAGCAGGA GGTGGCTCAG GTGGCCAGTG CCATCCTCTC CCTCAGGCGA
ACTCTCCAGG GCCTGCAGCT GGATCTGCCC CTGGAGGAGG AGACGTTGTC CCTTCCGAGA
GACCTGGAGA GTCTTGACAG AAGCTTCAAT GGTCTCCTTA CTATGTATCA GAGGAAGAGG
GAGCAGTTTG AAAAAATAAG CAGTGCTGAT CCTTCAGGAG CCTTCCGGAT GCTGAGCACA
GCCTACGAGC AGTCAGCCCA GGCTGCTCAG CAGGTCTCCG ACAGCTCGCG CCTTTTGGAC
CAGCTCAGGG ACAGCCGGAG AGAGGCAGAG AGGCTGGTGC GGCAGGCGGG AGGAGGAGGA
GGCACCGGCA GCCCCAAGCT TGTGGCCCTG AGGCTGGAGA TGTCTTCGTT GCCTGACCTG
ACACCCACCT TCAACAAGCT CTGTGGCAAC TCCAGGCAGA TGGCTTGCAC CCCAATATCA
TGCCCTGGTG AGCTATGTCC CCAAGACAAT GGCACAGCCT GTGGCTCCCG CTGCAGGGGT
GTCCTTCCCA GGGCCGGTGG GGCCTTCTTG ATGGCGGGGC AGGTGGCTGA GCAGCTGCGG
GGCTTCAATG CCCAGCTCCA GCGGACCAGG CAGATGATTA GGGCAGCCGA GGAATCTGCC
TCACAGATTC AATCCAGTGC CCAGCGCTTG GAGACCCAGG TGAGCGCCAG CCGCTCCCAG
ATGGAGGAAG ATGTCAGACG CACACGGCTC CTAATCCAGC AGGTCCGGGA CTTCCTAACA
GACCCCGACA CTGATGCAGC CACTATCCAG GAGGTCAGCG AGGCCGTGCT GGCCCTGTGG
CTGCCCACAG ACTCAGCTAC TGTTCTGCAG AAGATGAATG AGATCCAGGC CATTGCAGCC
AGGCTCCCCA ACGTGGACTT GGTGCTGTCC CAGACCAAGC AGGACATTGC GCGTGCCCGC
CGGTTGCAGG CTGAGGCTGA GGAAGCCAGG AGCCGAGCCC ATGCAGTGGA GGGCCAGGTG
GAAGATGTGG TTGGGAACCT GCGGCAGGGG ACAGTGGCAC TGCAGGAAGC TCAGGACACC
ATGCAAGGCA CCAGCCGCTC CCTTCGGCTT ATCCAGGACA GGGTTGCTGA GGTTCAGCAG
GTACTGCGGC CAGCAGAAAA GCTGGTGACA AGCATGACCA AGCAGCTGGG TGACTTCTGG
ACACGGATGG AGGAGCTCCG CCACCAAGCC CGGCAGCAGG GGGCAGAGGC AGTCCAGGCC
CAGCAGCTTG CGGAAGGTGC CAGCGAGCAG GCATTGAGTG CCCAAGAGGG ATTTGAGAGA
ATAAAACAAA AGTATGCTGA GTTGAAGGAC CGGTTGGGTC AGAGTTCCAT GCTGGGTGAG
CAGGGTGCCC GGATCCAGAG TGTGAAGACA GAGGCAGAGG AGCTGTTTGG GGAGACCATG
GAGATGATGG ACAGGATGAA AGACATGGAG TTGGAGCTGC TGCGGGGCAG CCAGGCCATC
ATGCTGCGCT CAGCGGACCT GACAGGACTG GAGAAGCGTG TGGAGCAGAT CCGTGACCAC
ATCAATGGGC GCGTGCTCTA CTATGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17378
Clone ID Related Accession (Same CDS sequence) NM_000228 , NM_001017402 , NM_001127641 , XM_005273124
Accession Version NM_001017402.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product laminin subunit beta-3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN414822.1, BC075838.1, BX345166.2, BC058922.1 and AY035783.1. Transcript Variant: This variant (2) has an alternate 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC075838.1, AY035783.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGACCAT TCTTCCTCTT GTGTTTTGCC CTGCCTGGCC TCCTGCATGC CCAACAAGCC 
TGCTCCCGTG GGGCCTGCTA TCCACCTGTT GGGGACCTGC TTGTTGGGAG GACCCGGTTT
CTCCGAGCTT CATCTACCTG TGGACTGACC AAGCCTGAGA CCTACTGCAC CCAGTATGGC
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGCAGC CTCACAACTA CTACAGTCAC
CGAGTAGAGA ATGTGGCTTC ATCCTCCGGC CCCATGCGCT GGTGGCAGTC ACAGAATGAT
GTGAACCCTG TCTCTCTGCA GCTGGACCTG GACAGGAGAT TCCAGCTTCA AGAAGTCATG
ATGGAGTTCC AGGGGCCCAT GCCCGCCGGC ATGCTGATTG AGCGCTCCTC AGACTTCGGT
AAGACCTGGC GAGTGTACCA GTACCTGGCT GCCGACTGCA CCTCCACCTT CCCTCGGGTC
CGCCAGGGTC GGCCTCAGAG CTGGCAGGAT GTTCGGTGCC AGTCCCTGCC TCAGAGGCCT
AATGCACGCC TAAATGGGGG GAAGGTCCAA CTTAACCTTA TGGATTTAGT GTCTGGGATT
CCAGCAACTC AAAGTCAAAA AATTCAAGAG GTGGGGGAGA TCACAAACTT GAGAGTCAAT
TTCACCAGGC TGGCCCCTGT GCCCCAAAGG GGCTACCACC CTCCCAGCGC CTACTATGCT
GTGTCCCAGC TCCGTCTGCA GGGGAGCTGC TTCTGTCACG GCCATGCTGA TCGCTGCGCA
CCCAAGCCTG GGGCCTCTGC AGGCCCCTCC ACCGCTGTGC AGGTCCACGA TGTCTGTGTC
TGCCAGCACA ACACTGCCGG CCCAAATTGT GAGCGCTGTG CACCCTTCTA CAACAACCGG
CCCTGGAGAC CGGCGGAGGG CCAGGACGCC CATGAATGCC AAAGGTGCGA CTGCAATGGG
CACTCAGAGA CATGTCACTT TGACCCCGCT GTGTTTGCCG CCAGCCAGGG GGCATATGGA
GGTGTGTGTG ACAATTGCCG GGACCACACC GAAGGCAAGA ACTGTGAGCG GTGTCAGCTG
CACTATTTCC GGAACCGGCG CCCGGGAGCT TCCATTCAGG AGACCTGCAT CTCCTGCGAG
TGTGATCCGG ATGGGGCAGT GCCAGGGGCT CCCTGTGACC CAGTGACCGG GCAGTGTGTG
TGCAAGGAGC ATGTGCAGGG AGAGCGCTGT GACCTATGCA AGCCGGGCTT CACTGGACTC
ACCTACGCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA ACATCCTGGG GTCCCGGAGG
GACATGCCGT GTGACGAGGA GAGTGGGCGC TGCCTTTGTC TGCCCAACGT GGTGGGTCCC
AAATGTGACC AGTGTGCTCC CTACCACTGG AAGCTGGCCA GTGGCCAGGG CTGTGAACCG
TGTGCCTGCG ACCCGCACAA CTCCCTCAGC CCACAGTGCA ACCAGTTCAC AGGGCAGTGC
CCCTGTCGGG AAGGCTTTGG TGGCCTGATG TGCAGCGCTG CAGCCATCCG CCAGTGTCCA
GACCGGACCT ATGGAGACGT GGCCACAGGA TGCCGAGCCT GTGACTGTGA TTTCCGGGGA
ACAGAGGGCC CGGGCTGCGA CAAGGCATCA GGCCGCTGCC TCTGCCGCCC TGGCTTGACC
GGGCCCCGCT GTGACCAGTG CCAGCGAGGC TACTGTAATC GCTACCCGGT GTGCGTGGCC
TGCCACCCTT GCTTCCAGAC CTATGATGCG GACCTCCGGG AGCAGGCCCT GCGCTTTGGT
AGACTCCGCA ATGCCACCGC CAGCCTGTGG TCAGGGCCTG GGCTGGAGGA CCGTGGCCTG
GCCTCCCGGA TCCTAGATGC AAAGAGTAAG ATTGAGCAGA TCCGAGCAGT TCTCAGCAGC
CCCGCAGTCA CAGAGCAGGA GGTGGCTCAG GTGGCCAGTG CCATCCTCTC CCTCAGGCGA
ACTCTCCAGG GCCTGCAGCT GGATCTGCCC CTGGAGGAGG AGACGTTGTC CCTTCCGAGA
GACCTGGAGA GTCTTGACAG AAGCTTCAAT GGTCTCCTTA CTATGTATCA GAGGAAGAGG
GAGCAGTTTG AAAAAATAAG CAGTGCTGAT CCTTCAGGAG CCTTCCGGAT GCTGAGCACA
GCCTACGAGC AGTCAGCCCA GGCTGCTCAG CAGGTCTCCG ACAGCTCGCG CCTTTTGGAC
CAGCTCAGGG ACAGCCGGAG AGAGGCAGAG AGGCTGGTGC GGCAGGCGGG AGGAGGAGGA
GGCACCGGCA GCCCCAAGCT TGTGGCCCTG AGGCTGGAGA TGTCTTCGTT GCCTGACCTG
ACACCCACCT TCAACAAGCT CTGTGGCAAC TCCAGGCAGA TGGCTTGCAC CCCAATATCA
TGCCCTGGTG AGCTATGTCC CCAAGACAAT GGCACAGCCT GTGGCTCCCG CTGCAGGGGT
GTCCTTCCCA GGGCCGGTGG GGCCTTCTTG ATGGCGGGGC AGGTGGCTGA GCAGCTGCGG
GGCTTCAATG CCCAGCTCCA GCGGACCAGG CAGATGATTA GGGCAGCCGA GGAATCTGCC
TCACAGATTC AATCCAGTGC CCAGCGCTTG GAGACCCAGG TGAGCGCCAG CCGCTCCCAG
ATGGAGGAAG ATGTCAGACG CACACGGCTC CTAATCCAGC AGGTCCGGGA CTTCCTAACA
GACCCCGACA CTGATGCAGC CACTATCCAG GAGGTCAGCG AGGCCGTGCT GGCCCTGTGG
CTGCCCACAG ACTCAGCTAC TGTTCTGCAG AAGATGAATG AGATCCAGGC CATTGCAGCC
AGGCTCCCCA ACGTGGACTT GGTGCTGTCC CAGACCAAGC AGGACATTGC GCGTGCCCGC
CGGTTGCAGG CTGAGGCTGA GGAAGCCAGG AGCCGAGCCC ATGCAGTGGA GGGCCAGGTG
GAAGATGTGG TTGGGAACCT GCGGCAGGGG ACAGTGGCAC TGCAGGAAGC TCAGGACACC
ATGCAAGGCA CCAGCCGCTC CCTTCGGCTT ATCCAGGACA GGGTTGCTGA GGTTCAGCAG
GTACTGCGGC CAGCAGAAAA GCTGGTGACA AGCATGACCA AGCAGCTGGG TGACTTCTGG
ACACGGATGG AGGAGCTCCG CCACCAAGCC CGGCAGCAGG GGGCAGAGGC AGTCCAGGCC
CAGCAGCTTG CGGAAGGTGC CAGCGAGCAG GCATTGAGTG CCCAAGAGGG ATTTGAGAGA
ATAAAACAAA AGTATGCTGA GTTGAAGGAC CGGTTGGGTC AGAGTTCCAT GCTGGGTGAG
CAGGGTGCCC GGATCCAGAG TGTGAAGACA GAGGCAGAGG AGCTGTTTGG GGAGACCATG
GAGATGATGG ACAGGATGAA AGACATGGAG TTGGAGCTGC TGCGGGGCAG CCAGGCCATC
ATGCTGCGCT CAGCGGACCT GACAGGACTG GAGAAGCGTG TGGAGCAGAT CCGTGACCAC
ATCAATGGGC GCGTGCTCTA CTATGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001017402.1
CDS391..3909
Misc Feature(1)259..261(+)
Misc Feature(2)448..1134(+)
Misc Feature(3)1135..1302(+)
Misc Feature(4)1138..1260(+)
Misc Feature(5)1333..1497(+)
Misc Feature(6)1336..1455(+)
Misc Feature(7)1522..1677(+)
Misc Feature(8)1525..1620(+)
Misc Feature(9)1678..1827(+)
Misc Feature(10)1681..1776(+)
Misc Feature(11)1828..1986(+)
Misc Feature(12)1831..1923(+)
Misc Feature(13)1987..2136(+)
Misc Feature(14)1990..2082(+)
Misc Feature(15)2125..2745(+)
Misc Feature(16)2746..2838(+)
Misc Feature(17)2839..3900(+)
Misc Feature(18)2920..3603(+)
Misc Feature(19)2974..3618(+)
Misc Feature(20)3007..3618(+)
Misc Feature(21)3265..3381(+)
Exon (1)1..418
Gene:LAMB3
Gene Synonym:
Exon (2)419..573
Gene:LAMB3
Gene Synonym:
Exon (3)574..688
Gene:LAMB3
Gene Synonym:
Exon (4)689..762
Gene:LAMB3
Gene Synonym:
Exon (5)763..954
Gene:LAMB3
Gene Synonym:
Exon (6)955..1018
Gene:LAMB3
Gene Synonym:
Exon (7)1019..1212
Gene:LAMB3
Gene Synonym:
Exon (8)1213..1333
Gene:LAMB3
Gene Synonym:
Exon (9)1334..1522
Gene:LAMB3
Gene Synonym:
Exon (10)1523..1678
Gene:LAMB3
Gene Synonym:
Exon (11)1679..1875
Gene:LAMB3
Gene Synonym:
Exon (12)1876..1987
Gene:LAMB3
Gene Synonym:
Exon (13)1988..2366
Gene:LAMB3
Gene Synonym:
Exon (14)2367..2527
Gene:LAMB3
Gene Synonym:
Exon (15)2528..2748
Gene:LAMB3
Gene Synonym:
Exon (16)2749..2946
Gene:LAMB3
Gene Synonym:
Exon (17)2947..3091
Gene:LAMB3
Gene Synonym:
Exon (18)3092..3299
Gene:LAMB3
Gene Synonym:
Exon (19)3300..3441
Gene:LAMB3
Gene Synonym:
Exon (20)3442..3618
Gene:LAMB3
Gene Synonym:
Exon (21)3619..3772
Gene:LAMB3
Gene Synonym:
Exon (22)3773..4310
Gene:LAMB3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001017402
Organism Homo sapiens (human)
Definition Homo sapiens laminin, beta 3 (LAMB3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001017402

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGACCAT TCTTCCTCTT GTGTTTTGCC CTGCCTGGCC TCCTGCATGC CCAACAAGCC 
TGCTCCCGTG GGGCCTGCTA TCCACCTGTT GGGGACCTGC TTGTTGGGAG GACCCGGTTT
CTCCGAGCTT CATCTACCTG TGGACTGACC AAGCCTGAGA CCTACTGCAC CCAGTATGGC
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGCAGC CTCACAACTA CTACAGTCAC
CGAGTAGAGA ATGTGGCTTC ATCCTCCGGC CCCATGCGCT GGTGGCAGTC ACAGAATGAT
GTGAACCCTG TCTCTCTGCA GCTGGACCTG GACAGGAGAT TCCAGCTTCA AGAAGTCATG
ATGGAGTTCC AGGGGCCCAT GCCCGCCGGC ATGCTGATTG AGCGCTCCTC AGACTTCGGT
AAGACCTGGC GAGTGTACCA GTACCTGGCT GCCGACTGCA CCTCCACCTT CCCTCGGGTC
CGCCAGGGTC GGCCTCAGAG CTGGCAGGAT GTTCGGTGCC AGTCCCTGCC TCAGAGGCCT
AATGCACGCC TAAATGGGGG GAAGGTCCAA CTTAACCTTA TGGATTTAGT GTCTGGGATT
CCAGCAACTC AAAGTCAAAA AATTCAAGAG GTGGGGGAGA TCACAAACTT GAGAGTCAAT
TTCACCAGGC TGGCCCCTGT GCCCCAAAGG GGCTACCACC CTCCCAGCGC CTACTATGCT
GTGTCCCAGC TCCGTCTGCA GGGGAGCTGC TTCTGTCACG GCCATGCTGA TCGCTGCGCA
CCCAAGCCTG GGGCCTCTGC AGGCCCCTCC ACCGCTGTGC AGGTCCACGA TGTCTGTGTC
TGCCAGCACA ACACTGCCGG CCCAAATTGT GAGCGCTGTG CACCCTTCTA CAACAACCGG
CCCTGGAGAC CGGCGGAGGG CCAGGACGCC CATGAATGCC AAAGGTGCGA CTGCAATGGG
CACTCAGAGA CATGTCACTT TGACCCCGCT GTGTTTGCCG CCAGCCAGGG GGCATATGGA
GGTGTGTGTG ACAATTGCCG GGACCACACC GAAGGCAAGA ACTGTGAGCG GTGTCAGCTG
CACTATTTCC GGAACCGGCG CCCGGGAGCT TCCATTCAGG AGACCTGCAT CTCCTGCGAG
TGTGATCCGG ATGGGGCAGT GCCAGGGGCT CCCTGTGACC CAGTGACCGG GCAGTGTGTG
TGCAAGGAGC ATGTGCAGGG AGAGCGCTGT GACCTATGCA AGCCGGGCTT CACTGGACTC
ACCTACGCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA ACATCCTGGG GTCCCGGAGG
GACATGCCGT GTGACGAGGA GAGTGGGCGC TGCCTTTGTC TGCCCAACGT GGTGGGTCCC
AAATGTGACC AGTGTGCTCC CTACCACTGG AAGCTGGCCA GTGGCCAGGG CTGTGAACCG
TGTGCCTGCG ACCCGCACAA CTCCCTCAGC CCACAGTGCA ACCAGTTCAC AGGGCAGTGC
CCCTGTCGGG AAGGCTTTGG TGGCCTGATG TGCAGCGCTG CAGCCATCCG CCAGTGTCCA
GACCGGACCT ATGGAGACGT GGCCACAGGA TGCCGAGCCT GTGACTGTGA TTTCCGGGGA
ACAGAGGGCC CGGGCTGCGA CAAGGCATCA GGCCGCTGCC TCTGCCGCCC TGGCTTGACC
GGGCCCCGCT GTGACCAGTG CCAGCGAGGC TACTGTAATC GCTACCCGGT GTGCGTGGCC
TGCCACCCTT GCTTCCAGAC CTATGATGCG GACCTCCGGG AGCAGGCCCT GCGCTTTGGT
AGACTCCGCA ATGCCACCGC CAGCCTGTGG TCAGGGCCTG GGCTGGAGGA CCGTGGCCTG
GCCTCCCGGA TCCTAGATGC AAAGAGTAAG ATTGAGCAGA TCCGAGCAGT TCTCAGCAGC
CCCGCAGTCA CAGAGCAGGA GGTGGCTCAG GTGGCCAGTG CCATCCTCTC CCTCAGGCGA
ACTCTCCAGG GCCTGCAGCT GGATCTGCCC CTGGAGGAGG AGACGTTGTC CCTTCCGAGA
GACCTGGAGA GTCTTGACAG AAGCTTCAAT GGTCTCCTTA CTATGTATCA GAGGAAGAGG
GAGCAGTTTG AAAAAATAAG CAGTGCTGAT CCTTCAGGAG CCTTCCGGAT GCTGAGCACA
GCCTACGAGC AGTCAGCCCA GGCTGCTCAG CAGGTCTCCG ACAGCTCGCG CCTTTTGGAC
CAGCTCAGGG ACAGCCGGAG AGAGGCAGAG AGGCTGGTGC GGCAGGCGGG AGGAGGAGGA
GGCACCGGCA GCCCCAAGCT TGTGGCCCTG AGGCTGGAGA TGTCTTCGTT GCCTGACCTG
ACACCCACCT TCAACAAGCT CTGTGGCAAC TCCAGGCAGA TGGCTTGCAC CCCAATATCA
TGCCCTGGTG AGCTATGTCC CCAAGACAAT GGCACAGCCT GTGGCTCCCG CTGCAGGGGT
GTCCTTCCCA GGGCCGGTGG GGCCTTCTTG ATGGCGGGGC AGGTGGCTGA GCAGCTGCGG
GGCTTCAATG CCCAGCTCCA GCGGACCAGG CAGATGATTA GGGCAGCCGA GGAATCTGCC
TCACAGATTC AATCCAGTGC CCAGCGCTTG GAGACCCAGG TGAGCGCCAG CCGCTCCCAG
ATGGAGGAAG ATGTCAGACG CACACGGCTC CTAATCCAGC AGGTCCGGGA CTTCCTAACA
GACCCCGACA CTGATGCAGC CACTATCCAG GAGGTCAGCG AGGCCGTGCT GGCCCTGTGG
CTGCCCACAG ACTCAGCTAC TGTTCTGCAG AAGATGAATG AGATCCAGGC CATTGCAGCC
AGGCTCCCCA ACGTGGACTT GGTGCTGTCC CAGACCAAGC AGGACATTGC GCGTGCCCGC
CGGTTGCAGG CTGAGGCTGA GGAAGCCAGG AGCCGAGCCC ATGCAGTGGA GGGCCAGGTG
GAAGATGTGG TTGGGAACCT GCGGCAGGGG ACAGTGGCAC TGCAGGAAGC TCAGGACACC
ATGCAAGGCA CCAGCCGCTC CCTTCGGCTT ATCCAGGACA GGGTTGCTGA GGTTCAGCAG
GTACTGCGGC CAGCAGAAAA GCTGGTGACA AGCATGACCA AGCAGCTGGG TGACTTCTGG
ACACGGATGG AGGAGCTCCG CCACCAAGCC CGGCAGCAGG GGGCAGAGGC AGTCCAGGCC
CAGCAGCTTG CGGAAGGTGC CAGCGAGCAG GCATTGAGTG CCCAAGAGGG ATTTGAGAGA
ATAAAACAAA AGTATGCTGA GTTGAAGGAC CGGTTGGGTC AGAGTTCCAT GCTGGGTGAG
CAGGGTGCCC GGATCCAGAG TGTGAAGACA GAGGCAGAGG AGCTGTTTGG GGAGACCATG
GAGATGATGG ACAGGATGAA AGACATGGAG TTGGAGCTGC TGCGGGGCAG CCAGGCCATC
ATGCTGCGCT CAGCGGACCT GACAGGACTG GAGAAGCGTG TGGAGCAGAT CCGTGACCAC
ATCAATGGGC GCGTGCTCTA CTATGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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