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LDHA cDNA ORF clone, Homo sapiens (human)

Gene Symbol LDHA
Entrez Gene ID 3939
Full Name lactate dehydrogenase A
Synonyms GSD11, HEL-S-133P, LDHM, PIG19
General protein information
Preferred Names
L-lactate dehydrogenase A chain
Names
L-lactate dehydrogenase A chain
LDH-A
LDH-M
LDH muscle subunit
lactate dehydrogenase M
proliferation-inducing gene 19
renal carcinoma antigen NY-REN-59
cell proliferation-inducing gene 19 protein
NP_001128711.1
NP_001158886.1
NP_001158887.1
NP_001158888.1
NP_005557.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11p15.4

Summary The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]. lac of sum
Disorder MIM:

150000

Disorder Html: Glycogen storage disease XI, 612933 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001165414 NP_001158886 L-lactate dehydrogenase A chain isoform 3
NM_001165415 NP_001158887 L-lactate dehydrogenase A chain isoform 4
NM_005566 NP_005557 L-lactate dehydrogenase A chain isoform 1
NM_001135239 NP_001128711 L-lactate dehydrogenase A chain isoform 2
NM_001165416 NP_001158888 L-lactate dehydrogenase A chain isoform 5



Homo sapiens (human) LDHA NP_001158886.1
Pan troglodytes (chimpanzee) LDHA NP_001029268.1
Macaca mulatta (Rhesus monkey) LDHA XP_001086967.1
Canis lupus familiaris (dog) LDHA XP_534084.4
Bos taurus (cattle) LDHA NP_776524.1
Mus musculus (house mouse) Ldha NP_001129541.2
Gallus gallus (chicken) LDHA NP_990615.1
Danio rerio (zebrafish) ldha NP_571321.1
Drosophila melanogaster (fruit fly) ImpL3 NP_476581.1
Caenorhabditis elegans ldh-1 NP_496503.1
Arabidopsis thaliana (thale cress) AT4G17260 NP_193459.1
Xenopus (Silurana) tropicalis (western clawed frog) ldha NP_989153.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LDHA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LDHA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu16013 NM_001165414 Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu15988 NM_001165415 Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $216.30
$309.00
OHu18760
NM_005566 Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu15968 NM_001135239 Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu15696 NM_001165416 Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu16013
Clone ID Related Accession (Same CDS sequence) NM_001165414
Accession Version NM_001165414.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1086bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product L-lactate dehydrogenase A chain isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC392310.1, DC396757.1, AK298834.1, AK307477.1, AC084117.6 and BM995301.1. Transcript Variant: This variant (3) uses an alternate 5' exon that results in a distinct 5' UTR and causes translation initiation at an alternate start codon, compared to variant 1. The resulting isoform (3) has a longer N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298834.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGGTGAAC CCTCAGGAGG CTATACTTAC ACCCAAACGT CGATATTCCT TTTCCACGCT 
AAGATTCCTT TTGGTTCCAA GTCCAATATG GCAACTCTAA AGGATCAGCT GATTTATAAT
CTTCTAAAGG AAGAACAGAC CCCCCAGAAT AAGATTACAG TTGTTGGGGT TGGTGCTGTT
GGCATGGCCT GTGCCATCAG TATCTTAATG AAGGACTTGG CAGATGAACT TGCTCTTGTT
GATGTCATCG AAGACAAATT GAAGGGAGAG ATGATGGATC TCCAACATGG CAGCCTTTTC
CTTAGAACAC CAAAGATTGT CTCTGGCAAA GACTATAATG TAACTGCAAA CTCCAAGCTG
GTCATTATCA CGGCTGGGGC ACGTCAGCAA GAGGGAGAAA GCCGTCTTAA TTTGGTCCAG
CGTAACGTGA ACATCTTTAA ATTCATCATT CCTAATGTTG TAAAATACAG CCCGAACTGC
AAGTTGCTTA TTGTTTCAAA TCCAGTGGAT ATCTTGACCT ACGTGGCTTG GAAGATAAGT
GGTTTTCCCA AAAACCGTGT TATTGGAAGC GGTTGCAATC TGGATTCAGC CCGATTCCGT
TACCTAATGG GGGAAAGGCT GGGAGTTCAC CCATTAAGCT GTCATGGGTG GGTCCTTGGG
GAACATGGAG ATTCCAGTGT GCCTGTATGG AGTGGAATGA ATGTTGCTGG TGTCTCTCTG
AAGACTCTGC ACCCAGATTT AGGGACTGAT AAAGATAAGG AACAGTGGAA AGAGGTTCAC
AAGCAGGTGG TTGAGAGTGC TTATGAGGTG ATCAAACTCA AAGGCTACAC ATCCTGGGCT
ATTGGACTCT CTGTAGCAGA TTTGGCAGAG AGTATAATGA AGAATCTTAG GCGGGTGCAC
CCAGTTTCCA CCATGATTAA GGGTCTTTAC GGAATAAAGG ATGATGTCTT CCTTAGTGTT
CCTTGCATTT TGGGACAGAA TGGAATCTCA GACCTTGTGA AGGTGACTCT GACTTCTGAG
GAAGAGGCCC GTTTGAAGAA GAGTGCAGAT ACACTTTGGG GGATCCAAAA GGAGCTGCAA
TTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001158886.1
CDS283..1368
Misc Feature(1)274..276(+)
Misc Feature(2)424..1356(+)
Misc Feature(3)427..1284(+)
Misc Feature(4)442..1341(+)
Misc Feature(5)454..1125(+)
Misc Feature(6)466..1122(+)
Misc Feature(7)667..1113(+)
Exon (1)1..345
Gene:LDHA
Gene Synonym:
Exon (2)346..495
Gene:LDHA
Gene Synonym:
Exon (3)496..613
Gene:LDHA
Gene Synonym:
Exon (4)614..787
Gene:LDHA
Gene Synonym:
Exon (5)788..961
Gene:LDHA
Gene Synonym:
Exon (6)962..1079
Gene:LDHA
Gene Synonym:
Exon (7)1080..1203
Gene:LDHA
Gene Synonym:
Exon (8)1204..2305
Gene:LDHA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001165414
Organism Homo sapiens (human)
Definition Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001165414

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGGTGAAC CCTCAGGAGG CTATACTTAC ACCCAAACGT CGATATTCCT TTTCCACGCT 
AAGATTCCTT TTGGTTCCAA GTCCAATATG GCAACTCTAA AGGATCAGCT GATTTATAAT
CTTCTAAAGG AAGAACAGAC CCCCCAGAAT AAGATTACAG TTGTTGGGGT TGGTGCTGTT
GGCATGGCCT GTGCCATCAG TATCTTAATG AAGGACTTGG CAGATGAACT TGCTCTTGTT
GATGTCATCG AAGACAAATT GAAGGGAGAG ATGATGGATC TCCAACATGG CAGCCTTTTC
CTTAGAACAC CAAAGATTGT CTCTGGCAAA GACTATAATG TAACTGCAAA CTCCAAGCTG
GTCATTATCA CGGCTGGGGC ACGTCAGCAA GAGGGAGAAA GCCGTCTTAA TTTGGTCCAG
CGTAACGTGA ACATCTTTAA ATTCATCATT CCTAATGTTG TAAAATACAG CCCGAACTGC
AAGTTGCTTA TTGTTTCAAA TCCAGTGGAT ATCTTGACCT ACGTGGCTTG GAAGATAAGT
GGTTTTCCCA AAAACCGTGT TATTGGAAGC GGTTGCAATC TGGATTCAGC CCGATTCCGT
TACCTAATGG GGGAAAGGCT GGGAGTTCAC CCATTAAGCT GTCATGGGTG GGTCCTTGGG
GAACATGGAG ATTCCAGTGT GCCTGTATGG AGTGGAATGA ATGTTGCTGG TGTCTCTCTG
AAGACTCTGC ACCCAGATTT AGGGACTGAT AAAGATAAGG AACAGTGGAA AGAGGTTCAC
AAGCAGGTGG TTGAGAGTGC TTATGAGGTG ATCAAACTCA AAGGCTACAC ATCCTGGGCT
ATTGGACTCT CTGTAGCAGA TTTGGCAGAG AGTATAATGA AGAATCTTAG GCGGGTGCAC
CCAGTTTCCA CCATGATTAA GGGTCTTTAC GGAATAAAGG ATGATGTCTT CCTTAGTGTT
CCTTGCATTT TGGGACAGAA TGGAATCTCA GACCTTGTGA AGGTGACTCT GACTTCTGAG
GAAGAGGCCC GTTTGAAGAA GAGTGCAGAT ACACTTTGGG GGATCCAAAA GGAGCTGCAA
TTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu15988
Clone ID Related Accession (Same CDS sequence) NM_001165415
Accession Version NM_001165415.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 825bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product L-lactate dehydrogenase A chain isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB502157.1, AK130587.1, AK307477.1, AC084117.6 and BM995301.1. Transcript Variant: This variant (4) differs in the 3' UTR and has multiple differences in the 3' coding region, compared to variant 1, one of which results in a frameshift. The resulting isoform (4) lacks a segment of the LDH domain and has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK130587.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCAACTC TAAAGGATCA GCTGATTTAT AATCTTCTAA AGGAAGAACA GACCCCCCAG 
AATAAGATTA CAGTTGTTGG GGTTGGTGCT GTTGGCATGG CCTGTGCCAT CAGTATCTTA
ATGAAGGACT TGGCAGATGA ACTTGCTCTT GTTGATGTCA TCGAAGACAA ATTGAAGGGA
GAGATGATGG ATCTCCAACA TGGCAGCCTT TTCCTTAGAA CACCAAAGAT TGTCTCTGGC
AAAGACTATA ATGTAACTGC AAACTCCAAG CTGGTCATTA TCACGGCTGG GGCACGTCAG
CAAGAGGGAG AAAGCCGTCT TAATTTGGTC CAGCGTAACG TGAACATCTT TAAATTCATC
ATTCCTAATG TTGTAAAATA CAGCCCGAAC TGCAAGTTGC TTATTGTTTC AAATCCAGTG
GATATCTTGA CCTACGTGGC TTGGAAGATA AGTGGTTTTC CCAAAAACCG TGTTATTGGA
AGCGGTTGCA ATCTGGATTC AGCCCGATTC CGTTACCTAA TGGGGGAAAG GCTGGGAGTT
CACCCATTAA GCTGTCATGG GTGGGTCCTT GGGGAACATG GAGATTCCAG TGTGCCTGTA
TGGAGTGGAA TGAATGTTGC TGGTGTCTCT CTGAAGACTC TGCACCCAGA TTTAGGGACT
GATAAAGATA AGGAACAGTG GAAAGAGTGC AGATACACTT TGGGGGATCC AAAAGGAGCT
GCAATTTTAA AGTCTTCTGA TGTCATATCA TTTCACTGTC TAGGCTACAA CAGGATTCTA
GGTGGAGGTT GTGCATGTTG TCCTTTTTAT CTGATCTGTG ATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001158887.1
CDS273..1097
Misc Feature(1)135..137(+)
Misc Feature(2)276..278(+)
Misc Feature(3)285..287(+)
Misc Feature(4)300..302(+)
Misc Feature(5)312..314(+)
Misc Feature(6)327..>959(+)
Misc Feature(7)357..851(+)
Misc Feature(8)369..806(+)
Misc Feature(9)441..443(+)
Misc Feature(10)513..515(+)
Misc Feature(11)570..851(+)
Misc Feature(12)624..626(+)
Misc Feature(13)648..650(+)
Exon (1)1..248
Gene:LDHA
Gene Synonym:
Exon (2)249..398
Gene:LDHA
Gene Synonym:
Exon (3)399..516
Gene:LDHA
Gene Synonym:
Exon (4)517..690
Gene:LDHA
Gene Synonym:
Exon (5)691..864
Gene:LDHA
Gene Synonym:
Exon (6)865..959
Gene:LDHA
Gene Synonym:
Exon (7)960..1939
Gene:LDHA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001165415
Organism Homo sapiens (human)
Definition Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001165415

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCAACTC TAAAGGATCA GCTGATTTAT AATCTTCTAA AGGAAGAACA GACCCCCCAG 
AATAAGATTA CAGTTGTTGG GGTTGGTGCT GTTGGCATGG CCTGTGCCAT CAGTATCTTA
ATGAAGGACT TGGCAGATGA ACTTGCTCTT GTTGATGTCA TCGAAGACAA ATTGAAGGGA
GAGATGATGG ATCTCCAACA TGGCAGCCTT TTCCTTAGAA CACCAAAGAT TGTCTCTGGC
AAAGACTATA ATGTAACTGC AAACTCCAAG CTGGTCATTA TCACGGCTGG GGCACGTCAG
CAAGAGGGAG AAAGCCGTCT TAATTTGGTC CAGCGTAACG TGAACATCTT TAAATTCATC
ATTCCTAATG TTGTAAAATA CAGCCCGAAC TGCAAGTTGC TTATTGTTTC AAATCCAGTG
GATATCTTGA CCTACGTGGC TTGGAAGATA AGTGGTTTTC CCAAAAACCG TGTTATTGGA
AGCGGTTGCA ATCTGGATTC AGCCCGATTC CGTTACCTAA TGGGGGAAAG GCTGGGAGTT
CACCCATTAA GCTGTCATGG GTGGGTCCTT GGGGAACATG GAGATTCCAG TGTGCCTGTA
TGGAGTGGAA TGAATGTTGC TGGTGTCTCT CTGAAGACTC TGCACCCAGA TTTAGGGACT
GATAAAGATA AGGAACAGTG GAAAGAGTGC AGATACACTT TGGGGGATCC AAAAGGAGCT
GCAATTTTAA AGTCTTCTGA TGTCATATCA TTTCACTGTC TAGGCTACAA CAGGATTCTA
GGTGGAGGTT GTGCATGTTG TCCTTTTTAT CTGATCTGTG ATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18760
Clone ID Related Accession (Same CDS sequence) NM_005566
Accession Version NM_005566.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 999bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product L-lactate dehydrogenase A chain isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB502157.1, BC067223.1, BG424022.1, AC084117.6 and BM995301.1. This sequence is a reference standard in the RefSeqGene project. On Sep 27, 2008 this sequence version replaced gi:171846254. Transcript Variant: This variant (1) encodes the predominant isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK130035.1, BC067223.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCAACTC TAAAGGATCA GCTGATTTAT AATCTTCTAA AGGAAGAACA GACCCCCCAG 
AATAAGATTA CAGTTGTTGG GGTTGGTGCT GTTGGCATGG CCTGTGCCAT CAGTATCTTA
ATGAAGGACT TGGCAGATGA ACTTGCTCTT GTTGATGTCA TCGAAGACAA ATTGAAGGGA
GAGATGATGG ATCTCCAACA TGGCAGCCTT TTCCTTAGAA CACCAAAGAT TGTCTCTGGC
AAAGACTATA ATGTAACTGC AAACTCCAAG CTGGTCATTA TCACGGCTGG GGCACGTCAG
CAAGAGGGAG AAAGCCGTCT TAATTTGGTC CAGCGTAACG TGAACATCTT TAAATTCATC
ATTCCTAATG TTGTAAAATA CAGCCCGAAC TGCAAGTTGC TTATTGTTTC AAATCCAGTG
GATATCTTGA CCTACGTGGC TTGGAAGATA AGTGGTTTTC CCAAAAACCG TGTTATTGGA
AGCGGTTGCA ATCTGGATTC AGCCCGATTC CGTTACCTAA TGGGGGAAAG GCTGGGAGTT
CACCCATTAA GCTGTCATGG GTGGGTCCTT GGGGAACATG GAGATTCCAG TGTGCCTGTA
TGGAGTGGAA TGAATGTTGC TGGTGTCTCT CTGAAGACTC TGCACCCAGA TTTAGGGACT
GATAAAGATA AGGAACAGTG GAAAGAGGTT CACAAGCAGG TGGTTGAGAG TGCTTATGAG
GTGATCAAAC TCAAAGGCTA CACATCCTGG GCTATTGGAC TCTCTGTAGC AGATTTGGCA
GAGAGTATAA TGAAGAATCT TAGGCGGGTG CACCCAGTTT CCACCATGAT TAAGGGTCTT
TACGGAATAA AGGATGATGT CTTCCTTAGT GTTCCTTGCA TTTTGGGACA GAATGGAATC
TCAGACCTTG TGAAGGTGAC TCTGACTTCT GAGGAAGAGG CCCGTTTGAA GAAGAGTGCA
GATACACTTT GGGGGATCCA AAAGGAGCTG CAATTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005557.1
CDS273..1271
Misc Feature(1)135..137(+)
Misc Feature(2)276..278(+)
Misc Feature(3)285..287(+)
Misc Feature(4)300..302(+)
Misc Feature(5)312..314(+)
Misc Feature(6)327..1259(+)
Misc Feature(7)330..1187(+)
Misc Feature(8)345..1244(+)
Misc Feature(9)357..1028(+)
Misc Feature(10)369..1025(+)
Misc Feature(11)441..443(+)
Misc Feature(12)513..515(+)
Misc Feature(13)570..1016(+)
Misc Feature(14)624..626(+)
Misc Feature(15)648..650(+)
Misc Feature(16)987..989(+)
Misc Feature(17)987..989(+)
Misc Feature(18)1224..1226(+)
Exon (1)1..248
Gene:LDHA
Gene Synonym:
Exon (2)249..398
Gene:LDHA
Gene Synonym:
Exon (3)399..516
Gene:LDHA
Gene Synonym:
Exon (4)517..690
Gene:LDHA
Gene Synonym:
Exon (5)691..864
Gene:LDHA
Gene Synonym:
Exon (6)865..982
Gene:LDHA
Gene Synonym:
Exon (7)983..1106
Gene:LDHA
Gene Synonym:
Exon (8)1107..2208
Gene:LDHA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_005566
Organism Homo sapiens (human)
Definition Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005566

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCAACTC TAAAGGATCA GCTGATTTAT AATCTTCTAA AGGAAGAACA GACCCCCCAG 
AATAAGATTA CAGTTGTTGG GGTTGGTGCT GTTGGCATGG CCTGTGCCAT CAGTATCTTA
ATGAAGGACT TGGCAGATGA ACTTGCTCTT GTTGATGTCA TCGAAGACAA ATTGAAGGGA
GAGATGATGG ATCTCCAACA TGGCAGCCTT TTCCTTAGAA CACCAAAGAT TGTCTCTGGC
AAAGACTATA ATGTAACTGC AAACTCCAAG CTGGTCATTA TCACGGCTGG GGCACGTCAG
CAAGAGGGAG AAAGCCGTCT TAATTTGGTC CAGCGTAACG TGAACATCTT TAAATTCATC
ATTCCTAATG TTGTAAAATA CAGCCCGAAC TGCAAGTTGC TTATTGTTTC AAATCCAGTG
GATATCTTGA CCTACGTGGC TTGGAAGATA AGTGGTTTTC CCAAAAACCG TGTTATTGGA
AGCGGTTGCA ATCTGGATTC AGCCCGATTC CGTTACCTAA TGGGGGAAAG GCTGGGAGTT
CACCCATTAA GCTGTCATGG GTGGGTCCTT GGGGAACATG GAGATTCCAG TGTGCCTGTA
TGGAGTGGAA TGAATGTTGC TGGTGTCTCT CTGAAGACTC TGCACCCAGA TTTAGGGACT
GATAAAGATA AGGAACAGTG GAAAGAGGTT CACAAGCAGG TGGTTGAGAG TGCTTATGAG
GTGATCAAAC TCAAAGGCTA CACATCCTGG GCTATTGGAC TCTCTGTAGC AGATTTGGCA
GAGAGTATAA TGAAGAATCT TAGGCGGGTG CACCCAGTTT CCACCATGAT TAAGGGTCTT
TACGGAATAA AGGATGATGT CTTCCTTAGT GTTCCTTGCA TTTTGGGACA GAATGGAATC
TCAGACCTTG TGAAGGTGAC TCTGACTTCT GAGGAAGAGG CCCGTTTGAA GAAGAGTGCA
GATACACTTT GGGGGATCCA AAAGGAGCTG CAATTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu15968
Clone ID Related Accession (Same CDS sequence) NM_001135239
Accession Version NM_001135239.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 825bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product L-lactate dehydrogenase A chain isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB502157.1, AK296667.1, AC084117.6 and BM995301.1. Transcript Variant: This variant (2) lacks an in-frame exon in the central coding region, compared to variant 1. The resulting isoform (2) lacks a segment of the LDH domain but has the same N- and C-termini, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296667.1, BU192904.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCAACTC TAAAGGATCA GCTGATTTAT AATCTTCTAA AGGAAGAACA GACCCCCCAG 
AATAAGATTA CAGTTGTTGG GGTTGGTGCT GTTGGCATGG CCTGTGCCAT CAGTATCTTA
ATGAAGGACT TGGCAGATGA ACTTGCTCTT GTTGATGTCA TCGAAGACAA ATTGAAGGGA
GAGATGATGG ATCTCCAACA TGGCAGCCTT TTCCTTAGAA CACCAAAGAT TGTCTCTGGC
AAAGTGGATA TCTTGACCTA CGTGGCTTGG AAGATAAGTG GTTTTCCCAA AAACCGTGTT
ATTGGAAGCG GTTGCAATCT GGATTCAGCC CGATTCCGTT ACCTAATGGG GGAAAGGCTG
GGAGTTCACC CATTAAGCTG TCATGGGTGG GTCCTTGGGG AACATGGAGA TTCCAGTGTG
CCTGTATGGA GTGGAATGAA TGTTGCTGGT GTCTCTCTGA AGACTCTGCA CCCAGATTTA
GGGACTGATA AAGATAAGGA ACAGTGGAAA GAGGTTCACA AGCAGGTGGT TGAGAGTGCT
TATGAGGTGA TCAAACTCAA AGGCTACACA TCCTGGGCTA TTGGACTCTC TGTAGCAGAT
TTGGCAGAGA GTATAATGAA GAATCTTAGG CGGGTGCACC CAGTTTCCAC CATGATTAAG
GGTCTTTACG GAATAAAGGA TGATGTCTTC CTTAGTGTTC CTTGCATTTT GGGACAGAAT
GGAATCTCAG ACCTTGTGAA GGTGACTCTG ACTTCTGAGG AAGAGGCCCG TTTGAAGAAG
AGTGCAGATA CACTTTGGGG GATCCAAAAG GAGCTGCAAT TTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001128711.1
CDS273..1097
Misc Feature(1)135..137(+)
Misc Feature(2)327..1085(+)
Misc Feature(3)330..1013(+)
Misc Feature(4)345..1070(+)
Misc Feature(5)357..854(+)
Misc Feature(6)369..851(+)
Misc Feature(7)603..842(+)
Exon (1)1..248
Gene:LDHA
Gene Synonym:
Exon (2)249..398
Gene:LDHA
Gene Synonym:
Exon (3)399..516
Gene:LDHA
Gene Synonym:
Exon (4)517..690
Gene:LDHA
Gene Synonym:
Exon (5)691..808
Gene:LDHA
Gene Synonym:
Exon (6)809..932
Gene:LDHA
Gene Synonym:
Exon (7)933..2034
Gene:LDHA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001135239
Organism Homo sapiens (human)
Definition Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001135239

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCAACTC TAAAGGATCA GCTGATTTAT AATCTTCTAA AGGAAGAACA GACCCCCCAG 
AATAAGATTA CAGTTGTTGG GGTTGGTGCT GTTGGCATGG CCTGTGCCAT CAGTATCTTA
ATGAAGGACT TGGCAGATGA ACTTGCTCTT GTTGATGTCA TCGAAGACAA ATTGAAGGGA
GAGATGATGG ATCTCCAACA TGGCAGCCTT TTCCTTAGAA CACCAAAGAT TGTCTCTGGC
AAAGTGGATA TCTTGACCTA CGTGGCTTGG AAGATAAGTG GTTTTCCCAA AAACCGTGTT
ATTGGAAGCG GTTGCAATCT GGATTCAGCC CGATTCCGTT ACCTAATGGG GGAAAGGCTG
GGAGTTCACC CATTAAGCTG TCATGGGTGG GTCCTTGGGG AACATGGAGA TTCCAGTGTG
CCTGTATGGA GTGGAATGAA TGTTGCTGGT GTCTCTCTGA AGACTCTGCA CCCAGATTTA
GGGACTGATA AAGATAAGGA ACAGTGGAAA GAGGTTCACA AGCAGGTGGT TGAGAGTGCT
TATGAGGTGA TCAAACTCAA AGGCTACACA TCCTGGGCTA TTGGACTCTC TGTAGCAGAT
TTGGCAGAGA GTATAATGAA GAATCTTAGG CGGGTGCACC CAGTTTCCAC CATGATTAAG
GGTCTTTACG GAATAAAGGA TGATGTCTTC CTTAGTGTTC CTTGCATTTT GGGACAGAAT
GGAATCTCAG ACCTTGTGAA GGTGACTCTG ACTTCTGAGG AAGAGGCCCG TTTGAAGAAG
AGTGCAGATA CACTTTGGGG GATCCAAAAG GAGCTGCAAT TTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu15696
Clone ID Related Accession (Same CDS sequence) NM_001165416
Accession Version NM_001165416.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 726bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product L-lactate dehydrogenase A chain isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB502157.1, BG760757.1, AC084117.6 and BM995301.1. Transcript Variant: This variant (5) lacks an alternate exon in the 3' coding region, compared to variant 1, which results in a frameshift. The resulting isoform (5) lacks a segment of the LDH domain and has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL559956.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCAACTC TAAAGGATCA GCTGATTTAT AATCTTCTAA AGGAAGAACA GACCCCCCAG 
AATAAGATTA CAGTTGTTGG GGTTGGTGCT GTTGGCATGG CCTGTGCCAT CAGTATCTTA
ATGAAGGACT TGGCAGATGA ACTTGCTCTT GTTGATGTCA TCGAAGACAA ATTGAAGGGA
GAGATGATGG ATCTCCAACA TGGCAGCCTT TTCCTTAGAA CACCAAAGAT TGTCTCTGGC
AAAGACTATA ATGTAACTGC AAACTCCAAG CTGGTCATTA TCACGGCTGG GGCACGTCAG
CAAGAGGGAG AAAGCCGTCT TAATTTGGTC CAGCGTAACG TGAACATCTT TAAATTCATC
ATTCCTAATG TTGTAAAATA CAGCCCGAAC TGCAAGTTGC TTATTGTTTC AAATCCAGTG
GATATCTTGA CCTACGTGGC TTGGAAGATA AGTGGTTTTC CCAAAAACCG TGTTATTGGA
AGCGGTTGCA ATCTGGATTC AGCCCGATTC CGTTACCTAA TGGGGGAAAG GCTGGGAGTT
CACCCATTAA GCTGTCATGG GTGGGTCCTT GGGGAACATG GAGATTCCAG TGTGCCTGTA
TGGAGTGGAA TGAATGTTGC TGGTGTCTCT CTGAAGACTC TGCACCCAGA TTTAGGGACT
GATAAAGATA AGGAACAGTG GAAAGAGGTT CACAAGCAGG TGGTTGAGAG GGTCTTTACG
GAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001158888.1
CDS273..998
Misc Feature(1)135..137(+)
Misc Feature(2)276..278(+)
Misc Feature(3)285..287(+)
Misc Feature(4)300..302(+)
Misc Feature(5)312..314(+)
Misc Feature(6)327..>992(+)
Misc Feature(7)357..851(+)
Misc Feature(8)369..806(+)
Misc Feature(9)441..443(+)
Misc Feature(10)513..515(+)
Misc Feature(11)570..986(+)
Misc Feature(12)624..626(+)
Misc Feature(13)648..650(+)
Exon (1)1..248
Gene:LDHA
Gene Synonym:
Exon (2)249..398
Gene:LDHA
Gene Synonym:
Exon (3)399..516
Gene:LDHA
Gene Synonym:
Exon (4)517..690
Gene:LDHA
Gene Synonym:
Exon (5)691..864
Gene:LDHA
Gene Synonym:
Exon (6)865..982
Gene:LDHA
Gene Synonym:
Exon (7)983..2084
Gene:LDHA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001165416
Organism Homo sapiens (human)
Definition Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001165416

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCAACTC TAAAGGATCA GCTGATTTAT AATCTTCTAA AGGAAGAACA GACCCCCCAG 
AATAAGATTA CAGTTGTTGG GGTTGGTGCT GTTGGCATGG CCTGTGCCAT CAGTATCTTA
ATGAAGGACT TGGCAGATGA ACTTGCTCTT GTTGATGTCA TCGAAGACAA ATTGAAGGGA
GAGATGATGG ATCTCCAACA TGGCAGCCTT TTCCTTAGAA CACCAAAGAT TGTCTCTGGC
AAAGACTATA ATGTAACTGC AAACTCCAAG CTGGTCATTA TCACGGCTGG GGCACGTCAG
CAAGAGGGAG AAAGCCGTCT TAATTTGGTC CAGCGTAACG TGAACATCTT TAAATTCATC
ATTCCTAATG TTGTAAAATA CAGCCCGAAC TGCAAGTTGC TTATTGTTTC AAATCCAGTG
GATATCTTGA CCTACGTGGC TTGGAAGATA AGTGGTTTTC CCAAAAACCG TGTTATTGGA
AGCGGTTGCA ATCTGGATTC AGCCCGATTC CGTTACCTAA TGGGGGAAAG GCTGGGAGTT
CACCCATTAA GCTGTCATGG GTGGGTCCTT GGGGAACATG GAGATTCCAG TGTGCCTGTA
TGGAGTGGAA TGAATGTTGC TGGTGTCTCT CTGAAGACTC TGCACCCAGA TTTAGGGACT
GATAAAGATA AGGAACAGTG GAAAGAGGTT CACAAGCAGG TGGTTGAGAG GGTCTTTACG
GAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Attenuation of LDHA expression in cancer cells leads to redox-dependent alterations in cytoskeletal structure and cell migration
Cancer Lett. 338 (2), 255-266 (2013)
Arseneault R, Chien A, Newington JT, Rappon T, Harris R and Cumming RC.


book

Galloflavin suppresses lactate dehydrogenase activity and causes MYC downregulation in Burkitt lymphoma cells through NAD/NADH-dependent inhibition of sirtuin-1
Anticancer Drugs 24 (8), 862-870 (2013)
Vettraino M, Manerba M, Govoni M and Di Stefano G.


book

Low expression of dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin-related protein in non-Hodgkin lymphoma and significant correlations with lactic acid dehydrogenase and beta2-microglobulin
Biochem. Cell Biol. 91 (4), 214-220 (2013)
Zhang Z, Chen K, Yan L, Yang Z, Zhu Z, Chen C, Zeng J, Wei W, Qi X, Ren S and Zuo Y.


book

Evaluation of LDH-A and glutaminase inhibition in vivo by hyperpolarized 13C-pyruvate magnetic resonance spectroscopy of tumors
Cancer Res. 73 (14), 4190-4195 (2013)
Dutta P, Le A, Vander Jagt DL, Tsukamoto T, Martinez GV, Dang CV and Gillies RJ.


book

Estrogen-related receptor alpha modulates lactate dehydrogenase activity in thyroid tumors
PLoS ONE 8 (3), E58683 (2013)
Mirebeau-Prunier D, Le Pennec S, Jacques C, Fontaine JF, Gueguen N, Boutet-Bouzamondo N, Donnart A, Malthiery Y and Savagner F.


book

[Gene expression in lactate dehydrogenase-A subunit deficiency]
Rinsho Shinkeigaku 32 (10), 1087-1092 (1992)
Miyajima H, Shimizu T and Kaneko E.


book

Molecular analysis of genetic mutation in electrophoretic variant of human lactate dehydrogenase-A(M) subunit
Biochem. Int. 27 (6), 1051-1057 (1992)
Sudo K, Maekawa M, Shioya M, Ikeda K, Takahashi N, Isogai Y, Li SS, Kanno T, Machida K and Toriumi J.


book

Genotypic analysis of families with lactate dehydrogenase A (M) deficiency by selective DNA amplification
Hum. Genet. 88 (1), 34-38 (1991)
Maekawa M, Sudo K, Li SS and Kanno T.


book

Analysis of genetic mutations in human lactate dehydrogenase-A(M) deficiency using DNA conformation polymorphism in combination with polyacrylamide gradient gel and silver staining
Biochem. Biophys. Res. Commun. 180 (2), 1083-1090 (1991)
Maekawa M, Sudo K, Li SS and Kanno T.


book

Rhabdomyosarcoma-associated locus and MYOD1 are syntenic but separate loci on the short arm of human chromosome 11
Proc. Natl. Acad. Sci. U.S.A. 87 (6), 2182-2186 (1990)
Scrable HJ, Johnson DK, Rinchik EM and Cavenee WK.


 
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