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LEP leptin [Homo sapiens (human)]

Gene Symbol LEP
Entrez Gene ID 3952
Full Name leptin
Synonyms LEPD, OB, OBS
General protein information
Preferred Names
leptin
Names
leptin
obese protein
obesity factor
obese, mouse, homolog of
leptin (murine obesity homolog)
leptin (obesity homolog, mouse)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7q31.3

Summary This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

164160

Disorder Html: Obesity, severe, due to leptin deficiency (3); Obesity, morbid, with





The following LEP gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LEP gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu41550 XM_005250340 PREDICTED: Homo sapiens leptin (LEP), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99
OHu27387 NM_000230 Homo sapiens leptin (LEP), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu41550
Accession Version XM_005250340.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 501bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product leptin isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578814244. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
ATGCATTGGG GAACCCTGTG CGGATTCTTG TGGCTTTGGC CCTATCTTTT CTATGTCCAA 
GCTGTGCCCA TCCAAAAAGT CCAAGATGAC ACCAAAACCC TCATCAAGAC AATTGTCACC
AGGATCAATG ACATTTCACA CACGTCAGTC TCCTCCAAAC AGAAAGTCAC CGGTTTGGAC
TTCATTCCTG GGCTCCACCC CATCCTGACC TTATCCAAGA TGGACCAGAC ACTGGCAGTC
TACCAACAGA TCCTCACCAG TATGCCTTCC AGAAACGTGA TCCAAATATC CAACGACCTG
GAGAACCTCC GGGATCTTCT TCACGTGCTG GCCTTCTCTA AGAGCTGCCA CTTGCCCTGG
GCCAGTGGCC TGGAGACCTT GGACAGCCTG GGGGGTGTCC TGGAAGCTTC AGGCTACTCC
ACAGAGGTGG TGGCCCTGAG CAGGCTGCAG GGGTCTCTGC AGGACATGCT GTGGCAGCTG
GACCTCAGCC CTGGGTGCTG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005250397.1
CDS134..634
Misc Feature(1)197..631(+)
Translation
Position Chain Variation Link
17 17 c, g dbSNP:545535648
18 18 g, t dbSNP:560478489
26 26 c, g dbSNP:774487738
39 39 -, c dbSNP:566757746
45 45 a, g dbSNP:200487063
48 48 a, g dbSNP:201381696
53 53 a, t dbSNP:34104384
75 75 a, c dbSNP:762333794
80 80 a, g dbSNP:767804499
95 95 a, g dbSNP:2167270
96 96 c, t dbSNP:561831965
106 106 g, t dbSNP:778907825
119 119 a, g dbSNP:748355602
123 123 c, t dbSNP:368669939
139 139 c, t dbSNP:199879263
148 148 c, g, t dbSNP:142904532
154 154 c, t dbSNP:201523305
155 155 a, g dbSNP:200092598
161 161 c, t dbSNP:770122731
163 163 c, g dbSNP:775874401
165 165 a, g dbSNP:763338727
167 167 a, c dbSNP:767150017
169 169 c, t dbSNP:540732424
171 171 g, t dbSNP:76529182
177 177 a, g dbSNP:760165439
179 179 c, g dbSNP:765869630
182 182 c, t dbSNP:201067336
186 186 a, g dbSNP:148407750
191 191 c, g dbSNP:200179130
208 208 a, g dbSNP:13306517
217 217 a, g dbSNP:202007341
223 223 c, t dbSNP:200176785
231 231 a, c dbSNP:752496962
234 234 -, tca dbSNP:747703977
240 240 a, g dbSNP:111650508
261 261 a, c dbSNP:201494642
262 262 c, t dbSNP:777989230
265 265 c, t dbSNP:372064089
266 266 a, g dbSNP:145044661
267 267 a, t dbSNP:781301976
271 271 a, g dbSNP:746345055
274 274 -, c dbSNP:771590018
276 276 c, t dbSNP:770247453
277 277 a, g, t dbSNP:1800583
285 285 c, t dbSNP:776443424
293 293 a, c dbSNP:759056593
295 295 a, g dbSNP:138908051
301 301 c, t dbSNP:775557183
304 304 c, g, t dbSNP:199647957
305 305 a, g dbSNP:200575914
317 317 a, c dbSNP:751272426
322 322 -, gggc dbSNP:761170909
325 325 g, t dbSNP:761690570
334 334 c, g dbSNP:140510728
343 343 c, t dbSNP:200187656
349 349 c, t dbSNP:750649453
356 356 g, t dbSNP:200915360
364 364 a, g dbSNP:201665158
368 368 g, t dbSNP:756180416
369 369 c, t dbSNP:780163329
379 379 a, g dbSNP:754347841
380 380 c, t dbSNP:755477612
385 385 a, c dbSNP:779501960
386 386 c, t dbSNP:748408158
388 388 c, t dbSNP:772575402
392 392 a, t dbSNP:199838573
398 398 a, c dbSNP:780990529
404 404 a, c dbSNP:745576625
406 406 a, g dbSNP:201185232
409 409 c, t dbSNP:774906243
410 410 a, g dbSNP:17151919
412 412 a, g dbSNP:768428766
415 415 a, c, t dbSNP:200061184
424 424 c, t dbSNP:200983754
427 427 c, t dbSNP:202169453
428 428 a, g, t dbSNP:724159998
436 436 a, g dbSNP:369454561
439 439 a, c, g, t dbSNP:28954113
443 443 c, t dbSNP:104894023
445 445 g, t dbSNP:755102882
449 449 c, g dbSNP:779227922
451 451 g, t dbSNP:753250158
457 457 c, t dbSNP:758826659
458 458 a, g, t dbSNP:1800564
466 466 c, t dbSNP:538776321
472 472 c, t dbSNP:779780275
473 473 a, g dbSNP:201114917
478 478 c, t dbSNP:202017228
484 484 c, t dbSNP:748689932
490 490 c, t dbSNP:768219399
495 495 a, c dbSNP:774055408
496 496 c, t dbSNP:774152821
497 497 a, g dbSNP:200202828
502 502 c, t dbSNP:201058992
505 505 a, g dbSNP:199851800
510 510 c, t dbSNP:773031071
517 517 c, g dbSNP:760494076
520 520 c, t dbSNP:766459987
523 523 -, g dbSNP:771139087
524 524 c, g dbSNP:200343690
528 528 g, t dbSNP:759465484
529 529 c, t dbSNP:765418389
532 532 c, t dbSNP:372892330
539 539 g, t dbSNP:759018010
578 578 c, t dbSNP:764732607
583 583 a, c, g dbSNP:367606905
584 584 c, g dbSNP:757709363
585 585 a, g dbSNP:779508377
586 586 a, g dbSNP:749036875
587 587 a, t dbSNP:201434410
596 596 a, g dbSNP:754432094
610 610 c, g dbSNP:75506045
611 611 c, g dbSNP:748045988
612 612 g, t dbSNP:771956117
614 614 a, g dbSNP:191666811
618 618 g, t dbSNP:199506740
620 620 a, c dbSNP:770761500
622 622 c, t dbSNP:200819049
626 626 c, g dbSNP:759854910
628 628 a, g dbSNP:765203455
635 635 a, g dbSNP:775675640
638 638 c, t dbSNP:763150646
645 645 c, g dbSNP:573626161
649 649 c, t dbSNP:764638610
650 650 a, t dbSNP:752116983
655 655 c, t dbSNP:757560540
659 659 a, g dbSNP:757558286
667 667 c, t dbSNP:62481073
668 668 a, g dbSNP:28954114
682 682 -, ct dbSNP:777124610
685 685 g, t dbSNP:750557041
735 735 a, t dbSNP:754834974
738 738 a, g dbSNP:778946065
740 740 a, g dbSNP:200618442
773 773 c, t dbSNP:577639649
784 784 a, g dbSNP:28954115
793 793 c, t dbSNP:550735439
800 800 c, g dbSNP:200136016
817 817 c, g dbSNP:201075060
822 822 a, t dbSNP:527270386
825 825 c, t dbSNP:201660972
831 831 c, t dbSNP:199893150
838 838 c, t dbSNP:113405088
847 847 c, t dbSNP:202242436
863 863 c, g dbSNP:28954116
876 876 g, t dbSNP:140383020
878 878 a, g dbSNP:201702208
909 909 g, t dbSNP:746596592
917 917 a, c dbSNP:200360100
921 921 a, g dbSNP:28954117
924 924 c, t dbSNP:199600549
934 934 c, t dbSNP:191600447
947 947 c, t dbSNP:201349081
969 969 a, g dbSNP:199804145
994 994 a, g dbSNP:200748706
1000 1000 a, g, t dbSNP:28954118
1007 1007 a, g dbSNP:183801334
1022 1022 c, t dbSNP:200861626
1028 1028 a, g dbSNP:201995159
1034 1034 g, t dbSNP:17151922
1036 1036 c, t dbSNP:554578326
1038 1038 c, t dbSNP:201188621
1049 1049 g, t dbSNP:201395608
1083 1083 c, t dbSNP:200166765
1107 1107 c, t dbSNP:200893037
1123 1123 a, g dbSNP:201721861
1150 1150 a, c dbSNP:200203015
1156 1156 a, g dbSNP:201207134
1174 1174 c, t dbSNP:202240898
1183 1183 c, t dbSNP:200464984
1196 1196 a, g dbSNP:201563706
1215 1215 a, g dbSNP:201990018
1225 1225 a, c dbSNP:111880866
1239 1239 g, t dbSNP:560475085
1245 1245 c, g dbSNP:201425072
1264 1264 c, t dbSNP:199614091
1274 1274 a, g dbSNP:200819927
1287 1287 a, c dbSNP:201341056
1299 1299 a, g dbSNP:199838375
1300 1300 a, c dbSNP:200806188
1319 1319 a, g dbSNP:111365240
1331 1331 c, t dbSNP:199878146
1340 1340 a, t dbSNP:548729924
1351 1351 a, c dbSNP:545231600
1366 1366 c, t dbSNP:200680655
1391 1391 c, t dbSNP:192559065
1415 1415 c, g dbSNP:70940808
1419 1419 a, c, g dbSNP:114834517
1429 1429 g, t dbSNP:148774522
1448 1448 c, t dbSNP:201985046
1450 1450 c, t dbSNP:200206765
1475 1475 a, g dbSNP:201088427
1475 1475 -, g dbSNP:780548989
1481 1481 g, t dbSNP:202039764
1487 1487 c, g dbSNP:200167789
1532 1532 a, g, t dbSNP:201279950
1536 1536 a, g dbSNP:185570072
1541 1541 g, t dbSNP:202164197
1585 1585 a, c dbSNP:200497536
1589 1589 a, g dbSNP:779133187
1598 1598 a, t dbSNP:565610542
1601 1601 c, t dbSNP:201559406
1610 1610 a, c dbSNP:532593154
1619 1619 a, c, g dbSNP:41526944
1621 1621 a, g dbSNP:547611951
1623 1623 a, g dbSNP:200931015
1625 1625 a, t dbSNP:201170334
1636 1636 c, g, t dbSNP:199633642
1638 1638 c, g dbSNP:565819571
1644 1644 a, c dbSNP:138289049
1648 1648 c, g dbSNP:151325384
1661 1661 c, t dbSNP:200215279
1685 1685 a, g dbSNP:760855323
1710 1710 c, g dbSNP:200801798
1722 1722 c, g dbSNP:201924066
1730 1730 c, t dbSNP:199839608
1732 1732 c, t dbSNP:201176555
1758 1758 a, g dbSNP:3750034
1760 1760 a, g dbSNP:202170412
1766 1766 a, c dbSNP:200020946
1782 1782 c, t dbSNP:13306516
1785 1785 a, c dbSNP:201033365
1801 1801 a, t dbSNP:556176595
1817 1817 a, g dbSNP:201982184
1819 1819 c, t dbSNP:200556203
1820 1820 a, g dbSNP:778098438
1821 1821 a, g dbSNP:139781325
1829 1829 c, t dbSNP:554046612
1839 1839 c, t dbSNP:201089993
1840 1840 a, g dbSNP:190525190
1871 1871 c, t dbSNP:200221912
1877 1877 a, g dbSNP:6966536
1879 1879 c, g dbSNP:199847071
1885 1885 a, g dbSNP:200339808
1889 1889 c, t dbSNP:201147488
1894 1894 a, g dbSNP:144195028
1907 1907 a, g dbSNP:200961875
1915 1915 a, g dbSNP:146556007
1923 1923 c, t dbSNP:201773371
1934 1934 c, t dbSNP:199726163
1937 1937 g, t dbSNP:200614624
1945 1945 a, g dbSNP:201676475
1952 1952 c, g dbSNP:200129463
1961 1961 c, t dbSNP:201075088
1974 1974 c, t dbSNP:747336931
2008 2008 g, t dbSNP:201919665
2009 2009 g, t dbSNP:28959468
2011 2011 g, t dbSNP:201333921
2025 2025 a, g dbSNP:202155976
2028 2028 g, t dbSNP:200304667
2034 2034 c, t dbSNP:536502398
2062 2062 a, c dbSNP:76601079
2069 2069 c, t dbSNP:201699721
2074 2074 c, g dbSNP:200361533
2075 2075 a, g dbSNP:181791569
2077 2077 a, g dbSNP:771268201
2095 2095 g, t dbSNP:201153946
2116 2116 c, t dbSNP:199600873
2122 2122 a, c dbSNP:369703431
2145 2145 c, t dbSNP:200406083
2161 2161 c, g dbSNP:201435616
2162 2162 c, g dbSNP:28959469
2182 2182 c, t dbSNP:200744739
2183 2183 c, t dbSNP:201701012
2197 2197 c, t dbSNP:199509773
2213 2213 g, t dbSNP:200802392
2258 2258 a, g dbSNP:201769388
2265 2265 c, t dbSNP:200086800
2271 2271 a, g dbSNP:780861433
2274 2274 c, t dbSNP:201065449
2293 2293 a, g dbSNP:184444762
2302 2302 a, g dbSNP:200162106
2311 2311 a, c dbSNP:200893300
2312 2312 c, t dbSNP:188857005
2315 2315 a, g dbSNP:563847116
2338 2338 -, c dbSNP:34052732
2342 2342 a, c dbSNP:199904710
2348 2348 c, t dbSNP:201114403
2354 2354 a, g dbSNP:10954174
2360 2360 a, c dbSNP:200530653
2371 2371 c, t dbSNP:201439123
2372 2372 a, g dbSNP:201949587
2381 2381 c, t dbSNP:200411232
2388 2388 a, c dbSNP:181229468
2389 2389 a, c dbSNP:201426744
2391 2391 a, g dbSNP:199610954
2394 2394 a, g dbSNP:749746610
2400 2400 c, t dbSNP:200617385
2401 2401 a, t dbSNP:28959470
2412 2412 a, g dbSNP:768194111
2459 2459 c, t dbSNP:199792491
2468 2468 -, g dbSNP:200090823
2469 2469 a, g dbSNP:185532950
2475 2475 c, g dbSNP:200916845
2476 2476 a, c dbSNP:201666212
2477 2477 a, g dbSNP:145064090
2495 2495 a, g dbSNP:199879026
2516 2516 a, g dbSNP:200681593
2520 2520 g, t dbSNP:201604408
2530 2530 c, t dbSNP:200054213
2532 2532 a, g dbSNP:547565590
2534 2534 a, g dbSNP:201077744
2536 2536 c, t dbSNP:202168818
2537 2537 a, g dbSNP:200128238
2550 2550 c, t dbSNP:201223818
2559 2559 c, t dbSNP:202049573
2584 2584 g, t dbSNP:200171215
2598 2598 a, g dbSNP:41528456
2613 2613 a, c dbSNP:554363383
2618 2618 c, g dbSNP:202157113
2621 2621 c, t dbSNP:576114940
2628 2628 g, t dbSNP:200527882
2633 2633 a, g dbSNP:774820661
2649 2649 a, c dbSNP:28959471
2651 2651 c, t dbSNP:199507100
2655 2655 c, t dbSNP:200822096
2678 2678 a, g dbSNP:558538763
2680 2680 a, g dbSNP:41434248
2683 2683 c, t dbSNP:199764881
2687 2687 a, g dbSNP:17617757
2715 2715 a, g dbSNP:201395425
2719 2719 g, t dbSNP:559480281
2727 2727 a, g dbSNP:200163732
2741 2741 c, t dbSNP:200866498
2744 2744 g, t dbSNP:187235717
2775 2775 c, t dbSNP:561089048
2778 2778 c, g dbSNP:766412099
2779 2779 c, t dbSNP:199897743
2807 2807 a, g dbSNP:542065935
2815 2815 a, g dbSNP:201230280
2830 2830 g, t dbSNP:201909251
2831 2831 a, g dbSNP:200005103
2833 2833 a, g dbSNP:201012185
2839 2839 a, g dbSNP:41457646
2847 2847 c, t dbSNP:200508994
2858 2858 a, g dbSNP:28959472
2871 2871 a, g dbSNP:202015551
2875 2875 c, g dbSNP:28959473
2879 2879 c, g dbSNP:201052331
2887 2887 a, c dbSNP:11761556
2896 2896 c, t dbSNP:200332242
2904 2904 a, g dbSNP:201089454
2907 2907 c, t dbSNP:199504835
2909 2909 c, t dbSNP:200863221
2937 2937 a, g dbSNP:201999428
2973 2973 g, t dbSNP:199693422
2994 2994 c, t dbSNP:200804321
3009 3009 a, g dbSNP:201666040
3011 3011 c, t dbSNP:200124981
3043 3043 a, g dbSNP:201044899
3044 3044 a, g dbSNP:28959474
3059 3059 c, t dbSNP:199890654
3061 3061 c, t dbSNP:201211541
3064 3064 a, t dbSNP:192232228
3071 3071 c, t dbSNP:536449594
3072 3072 c, t dbSNP:202238676
3088 3088 c, t dbSNP:572677784
3119 3119 g, t dbSNP:200492704
3137 3137 a, c, t dbSNP:200431904
3140 3140 -, a dbSNP:112344366
3140 3140 a, t dbSNP:750027837
3144 3144 a, g dbSNP:201652474
3155 3155 -, a dbSNP:57653562
3155 3155 a, g dbSNP:200356248
3156 3156 a, g, t dbSNP:201177389
3163 3163 c, t dbSNP:548406290
3170 3170 a, g dbSNP:199600363
3177 3177 c, g dbSNP:200429168
3180 3180 a, g dbSNP:201335762
3202 3202 a, g dbSNP:199831784
3208 3208 a, c dbSNP:537154218
3213 3213 a, c, g, t dbSNP:200738664
3229 3229 a, c, t dbSNP:28959475
3240 3240 a, g dbSNP:199500325
3269 3269 a, g dbSNP:200797675
3283 3283 c, t dbSNP:201995058
3313 3313 a, g dbSNP:553227670
3328 3328 c, t dbSNP:200071518
3368 3368 a, t dbSNP:551739864
3371 3371 c, t dbSNP:200842166
3372 3372 a, g dbSNP:201644572
3381 3381 a, t dbSNP:28959476
3384 3384 g, t dbSNP:200161125
3394 3394 c, t dbSNP:376558541
3433 3433 c, t dbSNP:200887574
3438 3438 -, aaa, aaaa dbSNP:562977237
3438 3438 a, t dbSNP:200515179
3454 3454 -, aaa dbSNP:386411254
3456 3456 -, aa, aaa dbSNP:56247456
3464 3464 a, t dbSNP:199905136

Target ORF information:

RefSeq Version XM_005250340
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens leptin (LEP), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005250340

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGCATTGGG GAACCCTGTG CGGATTCTTG TGGCTTTGGC CCTATCTTTT CTATGTCCAA 
GCTGTGCCCA TCCAAAAAGT CCAAGATGAC ACCAAAACCC TCATCAAGAC AATTGTCACC
AGGATCAATG ACATTTCACA CACGTCAGTC TCCTCCAAAC AGAAAGTCAC CGGTTTGGAC
TTCATTCCTG GGCTCCACCC CATCCTGACC TTATCCAAGA TGGACCAGAC ACTGGCAGTC
TACCAACAGA TCCTCACCAG TATGCCTTCC AGAAACGTGA TCCAAATATC CAACGACCTG
GAGAACCTCC GGGATCTTCT TCACGTGCTG GCCTTCTCTA AGAGCTGCCA CTTGCCCTGG
GCCAGTGGCC TGGAGACCTT GGACAGCCTG GGGGGTGTCC TGGAAGCTTC AGGCTACTCC
ACAGAGGTGG TGGCCCTGAG CAGGCTGCAG GGGTCTCTGC AGGACATGCT GTGGCAGCTG
GACCTCAGCC CTGGGTGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27387
Accession Version NM_000230.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 504bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu27387D_COA.pdf (pdf)
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product leptin precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA762132.1, U43653.1, AC018635.10 and BU752306.1. This sequence is a reference standard in the RefSeqGene project. On Mar 12, 2008 this sequence version replaced gi:4557714. Summary: This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U43653.1, BC060830.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
ATGCATTGGG GAACCCTGTG CGGATTCTTG TGGCTTTGGC CCTATCTTTT CTATGTCCAA 
GCTGTGCCCA TCCAAAAAGT CCAAGATGAC ACCAAAACCC TCATCAAGAC AATTGTCACC
AGGATCAATG ACATTTCACA CACGCAGTCA GTCTCCTCCA AACAGAAAGT CACCGGTTTG
GACTTCATTC CTGGGCTCCA CCCCATCCTG ACCTTATCCA AGATGGACCA GACACTGGCA
GTCTACCAAC AGATCCTCAC CAGTATGCCT TCCAGAAACG TGATCCAAAT ATCCAACGAC
CTGGAGAACC TCCGGGATCT TCTTCACGTG CTGGCCTTCT CTAAGAGCTG CCACTTGCCC
TGGGCCAGTG GCCTGGAGAC CTTGGACAGC CTGGGGGGTG TCCTGGAAGC TTCAGGCTAC
TCCACAGAGG TGGTGGCCCT GAGCAGGCTG CAGGGGTCTC TGCAGGACAT GCTGTGGCAG
CTGGACCTCA GCCCTGGGTG CTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000221.1
CDS58..561
Misc Feature(1)121..558(+)
Exon (1)1..29
Gene:LEP
Gene Synonym:
Exon (2)30..201
Gene:LEP
Gene Synonym:
Exon (3)202..3427
Gene:LEP
Gene Synonym:
Translation
Position Chain Variation Link
4 4 a, g dbSNP:767804499
19 19 a, g dbSNP:2167270
20 20 c, t dbSNP:561831965
30 30 g, t dbSNP:778907825
43 43 a, g dbSNP:748355602
47 47 c, t dbSNP:368669939
63 63 c, t dbSNP:199879263
72 72 c, g, t dbSNP:142904532
78 78 c, t dbSNP:201523305
79 79 a, g dbSNP:200092598
85 85 c, t dbSNP:770122731
87 87 c, g dbSNP:775874401
89 89 a, g dbSNP:763338727
91 91 a, c dbSNP:767150017
93 93 c, t dbSNP:540732424
95 95 g, t dbSNP:76529182
101 101 a, g dbSNP:760165439
103 103 c, g dbSNP:765869630
106 106 c, t dbSNP:201067336
110 110 a, g dbSNP:148407750
115 115 c, g dbSNP:200179130
132 132 a, g dbSNP:13306517
141 141 a, g dbSNP:202007341
147 147 c, t dbSNP:200176785
155 155 a, c dbSNP:752496962
158 158 -, tca dbSNP:747703977
164 164 a, g dbSNP:111650508
185 185 a, c dbSNP:201494642
186 186 c, t dbSNP:777989230
189 189 c, t dbSNP:372064089
190 190 a, g dbSNP:145044661
191 191 a, t dbSNP:781301976
195 195 a, g dbSNP:746345055
198 198 -, c dbSNP:771590018
200 200 c, t dbSNP:770247453
201 201 a, g, t dbSNP:1800583
212 212 c, t dbSNP:776443424
220 220 a, c dbSNP:759056593
222 222 a, g dbSNP:138908051
228 228 c, t dbSNP:775557183
231 231 c, g, t dbSNP:199647957
232 232 a, g dbSNP:200575914
244 244 a, c dbSNP:751272426
249 249 -, gggc dbSNP:761170909
252 252 g, t dbSNP:761690570
261 261 c, g dbSNP:140510728
270 270 c, t dbSNP:200187656
276 276 c, t dbSNP:750649453
283 283 g, t dbSNP:200915360
291 291 a, g dbSNP:201665158
295 295 g, t dbSNP:756180416
296 296 c, t dbSNP:780163329
306 306 a, g dbSNP:754347841
307 307 c, t dbSNP:755477612
312 312 a, c dbSNP:779501960
313 313 c, t dbSNP:748408158
315 315 c, t dbSNP:772575402
319 319 a, t dbSNP:199838573
325 325 a, c dbSNP:780990529
331 331 a, c dbSNP:745576625
333 333 a, g dbSNP:201185232
336 336 c, t dbSNP:774906243
337 337 a, g dbSNP:17151919
339 339 a, g dbSNP:768428766
342 342 a, c, t dbSNP:200061184
351 351 c, t dbSNP:200983754
354 354 c, t dbSNP:202169453
355 355 a, g, t dbSNP:724159998
363 363 a, g dbSNP:369454561
366 366 a, c, g, t dbSNP:28954113
370 370 c, t dbSNP:104894023
372 372 g, t dbSNP:755102882
376 376 c, g dbSNP:779227922
378 378 g, t dbSNP:753250158
384 384 c, t dbSNP:758826659
385 385 a, g, t dbSNP:1800564
393 393 c, t dbSNP:538776321
399 399 c, t dbSNP:779780275
400 400 a, g dbSNP:201114917
405 405 c, t dbSNP:202017228
411 411 c, t dbSNP:748689932
417 417 c, t dbSNP:768219399
422 422 a, c dbSNP:774055408
423 423 c, t dbSNP:774152821
424 424 a, g dbSNP:200202828
429 429 c, t dbSNP:201058992
432 432 a, g dbSNP:199851800
437 437 c, t dbSNP:773031071
444 444 c, g dbSNP:760494076
447 447 c, t dbSNP:766459987
450 450 -, g dbSNP:771139087
451 451 c, g dbSNP:200343690
455 455 g, t dbSNP:759465484
456 456 c, t dbSNP:765418389
459 459 c, t dbSNP:372892330
466 466 g, t dbSNP:759018010
505 505 c, t dbSNP:764732607
510 510 a, c, g dbSNP:367606905
511 511 c, g dbSNP:757709363
512 512 a, g dbSNP:779508377
513 513 a, g dbSNP:749036875
514 514 a, t dbSNP:201434410
523 523 a, g dbSNP:754432094
537 537 c, g dbSNP:75506045
538 538 c, g dbSNP:748045988
539 539 g, t dbSNP:771956117
541 541 a, g dbSNP:191666811
545 545 g, t dbSNP:199506740
547 547 a, c dbSNP:770761500
549 549 c, t dbSNP:200819049
553 553 c, g dbSNP:759854910
555 555 a, g dbSNP:765203455
562 562 a, g dbSNP:775675640
565 565 c, t dbSNP:763150646
572 572 c, g dbSNP:573626161
576 576 c, t dbSNP:764638610
577 577 a, t dbSNP:752116983
582 582 c, t dbSNP:757560540
586 586 a, g dbSNP:757558286
594 594 c, t dbSNP:62481073
595 595 a, g dbSNP:28954114
609 609 -, ct dbSNP:777124610
612 612 g, t dbSNP:750557041
662 662 a, t dbSNP:754834974
665 665 a, g dbSNP:778946065
667 667 a, g dbSNP:200618442
700 700 c, t dbSNP:577639649
711 711 a, g dbSNP:28954115
720 720 c, t dbSNP:550735439
727 727 c, g dbSNP:200136016
744 744 c, g dbSNP:201075060
749 749 a, t dbSNP:527270386
752 752 c, t dbSNP:201660972
758 758 c, t dbSNP:199893150
765 765 c, t dbSNP:113405088
774 774 c, t dbSNP:202242436
790 790 c, g dbSNP:28954116
803 803 g, t dbSNP:140383020
805 805 a, g dbSNP:201702208
836 836 g, t dbSNP:746596592
844 844 a, c dbSNP:200360100
848 848 a, g dbSNP:28954117
851 851 c, t dbSNP:199600549
861 861 c, t dbSNP:191600447
874 874 c, t dbSNP:201349081
896 896 a, g dbSNP:199804145
921 921 a, g dbSNP:200748706
927 927 a, g, t dbSNP:28954118
934 934 a, g dbSNP:183801334
949 949 c, t dbSNP:200861626
955 955 a, g dbSNP:201995159
961 961 g, t dbSNP:17151922
963 963 c, t dbSNP:554578326
965 965 c, t dbSNP:201188621
976 976 g, t dbSNP:201395608
1010 1010 c, t dbSNP:200166765
1034 1034 c, t dbSNP:200893037
1050 1050 a, g dbSNP:201721861
1077 1077 a, c dbSNP:200203015
1083 1083 a, g dbSNP:201207134
1101 1101 c, t dbSNP:202240898
1110 1110 c, t dbSNP:200464984
1123 1123 a, g dbSNP:201563706
1142 1142 a, g dbSNP:201990018
1152 1152 a, c dbSNP:111880866
1166 1166 g, t dbSNP:560475085
1172 1172 c, g dbSNP:201425072
1191 1191 c, t dbSNP:199614091
1201 1201 a, g dbSNP:200819927
1214 1214 a, c dbSNP:201341056
1226 1226 a, g dbSNP:199838375
1227 1227 a, c dbSNP:200806188
1246 1246 a, g dbSNP:111365240
1258 1258 c, t dbSNP:199878146
1267 1267 a, t dbSNP:548729924
1278 1278 a, c dbSNP:545231600
1293 1293 c, t dbSNP:200680655
1318 1318 c, t dbSNP:192559065
1342 1342 c, g dbSNP:70940808
1346 1346 a, c, g dbSNP:114834517
1356 1356 g, t dbSNP:148774522
1375 1375 c, t dbSNP:201985046
1377 1377 c, t dbSNP:200206765
1402 1402 a, g dbSNP:201088427
1402 1402 -, g dbSNP:780548989
1408 1408 g, t dbSNP:202039764
1414 1414 c, g dbSNP:200167789
1459 1459 a, g, t dbSNP:201279950
1463 1463 a, g dbSNP:185570072
1468 1468 g, t dbSNP:202164197
1512 1512 a, c dbSNP:200497536
1516 1516 a, g dbSNP:779133187
1525 1525 a, t dbSNP:565610542
1528 1528 c, t dbSNP:201559406
1537 1537 a, c dbSNP:532593154
1546 1546 a, c, g dbSNP:41526944
1548 1548 a, g dbSNP:547611951
1550 1550 a, g dbSNP:200931015
1552 1552 a, t dbSNP:201170334
1563 1563 c, g, t dbSNP:199633642
1565 1565 c, g dbSNP:565819571
1571 1571 a, c dbSNP:138289049
1575 1575 c, g dbSNP:151325384
1588 1588 c, t dbSNP:200215279
1612 1612 a, g dbSNP:760855323
1637 1637 c, g dbSNP:200801798
1649 1649 c, g dbSNP:201924066
1657 1657 c, t dbSNP:199839608
1659 1659 c, t dbSNP:201176555
1685 1685 a, g dbSNP:3750034
1687 1687 a, g dbSNP:202170412
1693 1693 a, c dbSNP:200020946
1709 1709 c, t dbSNP:13306516
1712 1712 a, c dbSNP:201033365
1728 1728 a, t dbSNP:556176595
1744 1744 a, g dbSNP:201982184
1746 1746 c, t dbSNP:200556203
1747 1747 a, g dbSNP:778098438
1748 1748 a, g dbSNP:139781325
1756 1756 c, t dbSNP:554046612
1766 1766 c, t dbSNP:201089993
1767 1767 a, g dbSNP:190525190
1798 1798 c, t dbSNP:200221912
1804 1804 a, g dbSNP:6966536
1806 1806 c, g dbSNP:199847071
1812 1812 a, g dbSNP:200339808
1816 1816 c, t dbSNP:201147488
1821 1821 a, g dbSNP:144195028
1834 1834 a, g dbSNP:200961875
1842 1842 a, g dbSNP:146556007
1850 1850 c, t dbSNP:201773371
1861 1861 c, t dbSNP:199726163
1864 1864 g, t dbSNP:200614624
1872 1872 a, g dbSNP:201676475
1879 1879 c, g dbSNP:200129463
1888 1888 c, t dbSNP:201075088
1901 1901 c, t dbSNP:747336931
1935 1935 g, t dbSNP:201919665
1936 1936 g, t dbSNP:28959468
1938 1938 g, t dbSNP:201333921
1952 1952 a, g dbSNP:202155976
1955 1955 g, t dbSNP:200304667
1961 1961 c, t dbSNP:536502398
1989 1989 a, c dbSNP:76601079
1996 1996 c, t dbSNP:201699721
2001 2001 c, g dbSNP:200361533
2002 2002 a, g dbSNP:181791569
2004 2004 a, g dbSNP:771268201
2022 2022 g, t dbSNP:201153946
2043 2043 c, t dbSNP:199600873
2049 2049 a, c dbSNP:369703431
2072 2072 c, t dbSNP:200406083
2088 2088 c, g dbSNP:201435616
2089 2089 c, g dbSNP:28959469
2109 2109 c, t dbSNP:200744739
2110 2110 c, t dbSNP:201701012
2124 2124 c, t dbSNP:199509773
2140 2140 g, t dbSNP:200802392
2185 2185 a, g dbSNP:201769388
2192 2192 c, t dbSNP:200086800
2198 2198 a, g dbSNP:780861433
2201 2201 c, t dbSNP:201065449
2220 2220 a, g dbSNP:184444762
2229 2229 a, g dbSNP:200162106
2238 2238 a, c dbSNP:200893300
2239 2239 c, t dbSNP:188857005
2242 2242 a, g dbSNP:563847116
2265 2265 -, c dbSNP:34052732
2269 2269 a, c dbSNP:199904710
2275 2275 c, t dbSNP:201114403
2281 2281 a, g dbSNP:10954174
2287 2287 a, c dbSNP:200530653
2298 2298 c, t dbSNP:201439123
2299 2299 a, g dbSNP:201949587
2308 2308 c, t dbSNP:200411232
2315 2315 a, c dbSNP:181229468
2316 2316 a, c dbSNP:201426744
2318 2318 a, g dbSNP:199610954
2321 2321 a, g dbSNP:749746610
2327 2327 c, t dbSNP:200617385
2328 2328 a, t dbSNP:28959470
2339 2339 a, g dbSNP:768194111
2386 2386 c, t dbSNP:199792491
2395 2395 -, g dbSNP:200090823
2396 2396 a, g dbSNP:185532950
2402 2402 c, g dbSNP:200916845
2403 2403 a, c dbSNP:201666212
2404 2404 a, g dbSNP:145064090
2422 2422 a, g dbSNP:199879026
2443 2443 a, g dbSNP:200681593
2447 2447 g, t dbSNP:201604408
2457 2457 c, t dbSNP:200054213
2459 2459 a, g dbSNP:547565590
2461 2461 a, g dbSNP:201077744
2463 2463 c, t dbSNP:202168818
2464 2464 a, g dbSNP:200128238
2477 2477 c, t dbSNP:201223818
2486 2486 c, t dbSNP:202049573
2511 2511 g, t dbSNP:200171215
2525 2525 a, g dbSNP:41528456
2540 2540 a, c dbSNP:554363383
2545 2545 c, g dbSNP:202157113
2548 2548 c, t dbSNP:576114940
2555 2555 g, t dbSNP:200527882
2560 2560 a, g dbSNP:774820661
2576 2576 a, c dbSNP:28959471
2578 2578 c, t dbSNP:199507100
2582 2582 c, t dbSNP:200822096
2605 2605 a, g dbSNP:558538763
2607 2607 a, g dbSNP:41434248
2610 2610 c, t dbSNP:199764881
2614 2614 a, g dbSNP:17617757
2642 2642 a, g dbSNP:201395425
2646 2646 g, t dbSNP:559480281
2654 2654 a, g dbSNP:200163732
2668 2668 c, t dbSNP:200866498
2671 2671 g, t dbSNP:187235717
2702 2702 c, t dbSNP:561089048
2705 2705 c, g dbSNP:766412099
2706 2706 c, t dbSNP:199897743
2734 2734 a, g dbSNP:542065935
2742 2742 a, g dbSNP:201230280
2757 2757 g, t dbSNP:201909251
2758 2758 a, g dbSNP:200005103
2760 2760 a, g dbSNP:201012185
2766 2766 a, g dbSNP:41457646
2774 2774 c, t dbSNP:200508994
2785 2785 a, g dbSNP:28959472
2798 2798 a, g dbSNP:202015551
2802 2802 c, g dbSNP:28959473
2806 2806 c, g dbSNP:201052331
2814 2814 a, c dbSNP:11761556
2823 2823 c, t dbSNP:200332242
2831 2831 a, g dbSNP:201089454
2834 2834 c, t dbSNP:199504835
2836 2836 c, t dbSNP:200863221
2864 2864 a, g dbSNP:201999428
2900 2900 g, t dbSNP:199693422
2921 2921 c, t dbSNP:200804321
2936 2936 a, g dbSNP:201666040
2938 2938 c, t dbSNP:200124981
2970 2970 a, g dbSNP:201044899
2971 2971 a, g dbSNP:28959474
2986 2986 c, t dbSNP:199890654
2988 2988 c, t dbSNP:201211541
2991 2991 a, t dbSNP:192232228
2998 2998 c, t dbSNP:536449594
2999 2999 c, t dbSNP:202238676
3015 3015 c, t dbSNP:572677784
3046 3046 g, t dbSNP:200492704
3064 3064 a, c, t dbSNP:200431904
3067 3067 -, a dbSNP:112344366
3067 3067 a, t dbSNP:750027837
3071 3071 a, g dbSNP:201652474
3082 3082 -, a dbSNP:57653562
3082 3082 a, g dbSNP:200356248
3083 3083 a, g, t dbSNP:201177389
3090 3090 c, t dbSNP:548406290
3097 3097 a, g dbSNP:199600363
3104 3104 c, g dbSNP:200429168
3107 3107 a, g dbSNP:201335762
3129 3129 a, g dbSNP:199831784
3135 3135 a, c dbSNP:537154218
3140 3140 a, c, g, t dbSNP:200738664
3156 3156 a, c, t dbSNP:28959475
3167 3167 a, g dbSNP:199500325
3196 3196 a, g dbSNP:200797675
3210 3210 c, t dbSNP:201995058
3240 3240 a, g dbSNP:553227670
3255 3255 c, t dbSNP:200071518
3295 3295 a, t dbSNP:551739864
3298 3298 c, t dbSNP:200842166
3299 3299 a, g dbSNP:201644572
3308 3308 a, t dbSNP:28959476
3311 3311 g, t dbSNP:200161125
3321 3321 c, t dbSNP:376558541
3360 3360 c, t dbSNP:200887574
3365 3365 -, aaa, aaaa dbSNP:562977237
3365 3365 a, t dbSNP:200515179
3381 3381 -, aaa dbSNP:386411254
3383 3383 -, aa, aaa dbSNP:56247456
3391 3391 a, t dbSNP:199905136

Target ORF information:

RefSeq Version NM_000230
Organism Homo sapiens (human)
Definition Homo sapiens leptin (LEP), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000230

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGCATTGGG GAACCCTGTG CGGATTCTTG TGGCTTTGGC CCTATCTTTT CTATGTCCAA 
GCTGTGCCCA TCCAAAAAGT CCAAGATGAC ACCAAAACCC TCATCAAGAC AATTGTCACC
AGGATCAATG ACATTTCACA CACGCAGTCA GTCTCCTCCA AACAGAAAGT CACCGGTTTG
GACTTCATTC CTGGGCTCCA CCCCATCCTG ACCTTATCCA AGATGGACCA GACACTGGCA
GTCTACCAAC AGATCCTCAC CAGTATGCCT TCCAGAAACG TGATCCAAAT ATCCAACGAC
CTGGAGAACC TCCGGGATCT TCTTCACGTG CTGGCCTTCT CTAAGAGCTG CCACTTGCCC
TGGGCCAGTG GCCTGGAGAC CTTGGACAGC CTGGGGGGTG TCCTGGAAGC TTCAGGCTAC
TCCACAGAGG TGGTGGCCCT GAGCAGGCTG CAGGGGTCTC TGCAGGACAT GCTGTGGCAG
CTGGACCTCA GCCCTGGGTG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.