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LMNA cDNA ORF clone, Homo sapiens (human)

Gene Symbol LMNA
Entrez Gene ID 4000
Full Name lamin A/C
Synonyms CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, PRO1
General protein information
Preferred Names
lamin
Names
lamin
70 kDa lamin
prelamin-A/C
lamin A/C-like 1
renal carcinoma antigen NY-REN-32
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q22

Summary The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]. lac of sum
Disorder MIM:

150330

Disorder Html: Emery-Dreifuss muscular dystrophy, AD, 181350 (3); Cardiomyopathy, dilated, 1A, 115200 (3); Lipodystrophy, familial partial, 151660 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_001282626 NP_001269555 lamin isoform A-delta50
NM_001282624 NP_001269553 lamin isoform E
NM_001257374 NP_001244303 lamin isoform D
NM_170708 NP_733822 lamin isoform A-delta10
NM_005572 NP_005563 lamin isoform C
NM_001282625 NP_001269554 lamin isoform C
NM_170707 NP_733821 lamin isoform A
XM_011509533 XP_011507835 lamin isoform X1
XM_011509534 XP_011507836 lamin isoform X2



Homo sapiens (human) LMNA NP_733821.1
Pan troglodytes (chimpanzee) LMNA NP_001092024.1
Macaca mulatta (Rhesus monkey) LMNA XP_001113604.2
Canis lupus familiaris (dog) LMNA XP_005622768.1
Bos taurus (cattle) LMNA NP_001029225.1
Mus musculus (house mouse) Lmna NP_001002011.2
Rattus norvegicus (Norway rat) Lmna NP_001002016.1
Danio rerio (zebrafish) lmna NP_694503.1
Xenopus (Silurana) tropicalis (western clawed frog) lmna NP_001039148.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LMNA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMNA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu27661 NM_001282626 Homo sapiens lamin A/C (LMNA), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $307.30
$439.00
OHu27626 NM_001282624 Homo sapiens lamin A/C (LMNA), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu22769 NM_001257374 Homo sapiens lamin A/C (LMNA), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu20696 NM_170708 Homo sapiens lamin A/C (LMNA), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu16808 NM_005572 Homo sapiens lamin A/C (LMNA), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $265.30
$379.00
OHu16808 NM_001282625 Homo sapiens lamin A/C (LMNA), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $265.30
$379.00
OHu21250 NM_170707 Homo sapiens lamin A/C (LMNA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $265.30
$379.00
OHu58735 XM_011509533 PREDICTED: Homo sapiens lamin A/C (LMNA), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu58736 XM_011509534 PREDICTED: Homo sapiens lamin A/C (LMNA), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu27661
Clone ID Related Accession (Same CDS sequence) NM_001282626
Accession Version NM_001282626.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1845bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-JUL-2015
Organism Homo sapiens (human)
Product lamin isoform A-delta50
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA551594.1, AY357727.1, BU685425.1, BU732343.1 and AI872233.1. Transcript Variant: This variant (7) uses an alternate 3' exon structure and thus differs in the 3' coding region and 3' UTR, compared to variant 1. This results in a shorter isoform (A-delta50, also known as progerin) with a distinct C-terminus when compared to isoform prelamin A. Although this isoform has been linked to Hutchinson-Gilford progeria syndrome, it is also found in unaffected individuals and thought to be linked to cellular terminal differentiation and physiological aging (see PubMed IDs: 12702809, 16645051, and 18060063). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY357727.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGAGACCC CGTCCCAGCG GCGCGCCACC CGCAGCGGGG CGCAGGCCAG CTCCACTCCG 
CTGTCGCCCA CCCGCATCAC CCGGCTGCAG GAGAAGGAGG ACCTGCAGGA GCTCAATGAT
CGCTTGGCGG TCTACATCGA CCGTGTGCGC TCGCTGGAAA CGGAGAACGC AGGGCTGCGC
CTTCGCATCA CCGAGTCTGA AGAGGTGGTC AGCCGCGAGG TGTCCGGCAT CAAGGCCGCC
TACGAGGCCG AGCTCGGGGA TGCCCGCAAG ACCCTTGACT CAGTAGCCAA GGAGCGCGCC
CGCCTGCAGC TGGAGCTGAG CAAAGTGCGT GAGGAGTTTA AGGAGCTGAA AGCGCGCAAT
ACCAAGAAGG AGGGTGACCT GATAGCTGCT CAGGCTCGGC TGAAGGACCT GGAGGCTCTG
CTGAACTCCA AGGAGGCCGC ACTGAGCACT GCTCTCAGTG AGAAGCGCAC GCTGGAGGGC
GAGCTGCATG ATCTGCGGGG CCAGGTGGCC AAGCTTGAGG CAGCCCTAGG TGAGGCCAAG
AAGCAACTTC AGGATGAGAT GCTGCGGCGG GTGGATGCTG AGAACAGGCT GCAGACCATG
AAGGAGGAAC TGGACTTCCA GAAGAACATC TACAGTGAGG AGCTGCGTGA GACCAAGCGC
CGTCATGAGA CCCGACTGGT GGAGATTGAC AATGGGAAGC AGCGTGAGTT TGAGAGCCGG
CTGGCGGATG CGCTGCAGGA ACTGCGGGCC CAGCATGAGG ACCAGGTGGA GCAGTATAAG
AAGGAGCTGG AGAAGACTTA TTCTGCCAAG CTGGACAATG CCAGGCAGTC TGCTGAGAGG
AACAGCAACC TGGTGGGGGC TGCCCACGAG GAGCTGCAGC AGTCGCGCAT CCGCATCGAC
AGCCTCTCTG CCCAGCTCAG CCAGCTCCAG AAGCAGCTGG CAGCCAAGGA GGCGAAGCTT
CGAGACCTGG AGGACTCACT GGCCCGTGAG CGGGACACCA GCCGGCGGCT GCTGGCGGAA
AAGGAGCGGG AGATGGCCGA GATGCGGGCA AGGATGCAGC AGCAGCTGGA CGAGTACCAG
GAGCTTCTGG ACATCAAGCT GGCCCTGGAC ATGGAGATCC ACGCCTACCG CAAGCTCTTG
GAGGGCGAGG AGGAGAGGCT ACGCCTGTCC CCCAGCCCTA CCTCGCAGCG CAGCCGTGGC
CGTGCTTCCT CTCACTCATC CCAGACACAG GGTGGGGGCA GCGTCACCAA AAAGCGCAAA
CTGGAGTCCA CTGAGAGCCG CAGCAGCTTC TCACAGCACG CACGCACTAG CGGGCGCGTG
GCCGTGGAGG AGGTGGATGA GGAGGGCAAG TTTGTCCGGC TGCGCAACAA GTCCAATGAG
GACCAGTCCA TGGGCAATTG GCAGATCAAG CGCCAGAATG GAGATGATCC CTTGCTGACT
TACCGGTTCC CACCAAAGTT CACCCTGAAG GCTGGGCAGG TGGTGACGAT CTGGGCTGCA
GGAGCTGGGG CCACCCACAG CCCCCCTACC GACCTGGTGT GGAAGGCACA GAACACCTGG
GGCTGCGGGA ACAGCCTGCG TACGGCTCTC ATCAACTCCA CTGGGGAAGA AGTGGCCATG
CGCAAGCTGG TGCGCTCAGT GACTGTGGTT GAGGACGACG AGGATGAGGA TGGAGATGAC
CTGCTCCATC ACCACCACGG CTCCCACTGC AGCAGCTCGG GGGACCCCGC TGAGTACAAC
CTGCGCTCGC GCACCGTGCT GTGCGGGACC TGCGGGCAGC CTGCCGACAA GGCATCTGCC
AGCGGCTCAG GAGCCCAGAG CCCCCAGAAC TGCAGCATCA TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001269555.1
CDS250..2094
Misc Feature(1)13..15(+)
Misc Feature(2)250..639(+)
Misc Feature(3)250..348(+)
Misc Feature(4)250..252(+)
Misc Feature(5)256..258(+)
Misc Feature(6)283..285(+)
Misc Feature(7)301..303(+)
Misc Feature(8)304..306(+)
Misc Feature(9)313..315(+)
Misc Feature(10)337..1407(+)
Misc Feature(11)343..693(+)
Misc Feature(12)343..345(+)
Misc Feature(13)343..345(+)
Misc Feature(14)349..1398(+)
Misc Feature(15)349..459(+)
Misc Feature(16)460..489(+)
Misc Feature(17)490..903(+)
Misc Feature(18)571..573(+)
Misc Feature(19)616..618(+)
Misc Feature(20)652..654(+)
Misc Feature(21)712..714(+)
Misc Feature(22)757..>1038(+)
Misc Feature(23)760..762(+)
Misc Feature(24)760..762(+)
Misc Feature(25)850..852(+)
Misc Feature(26)883..885(+)
Misc Feature(27)904..975(+)
Misc Feature(28)976..1398(+)
Misc Feature(29)1027..1029(+)
Misc Feature(30)1045..1047(+)
Misc Feature(31)1057..1059(+)
Misc Feature(32)1078..1080(+)
Misc Feature(33)1150..1152(+)
Misc Feature(34)1153..>1338(+)
Misc Feature(35)1168..1170(+)
Misc Feature(36)1180..1182(+)
Misc Feature(37)1222..1224(+)
Misc Feature(38)1237..1239(+)
Misc Feature(39)1417..1419(+)
Misc Feature(40)1423..1425(+)
Misc Feature(41)1432..1434(+)
Misc Feature(42)1456..1458(+)
Misc Feature(43)1459..1461(+)
Misc Feature(44)1468..1470(+)
Misc Feature(45)1489..1491(+)
Misc Feature(46)1498..1515(+)
Misc Feature(47)1540..1542(+)
Misc Feature(48)1546..1893(+)
Misc Feature(49)1597..1599(+)
Misc Feature(50)1618..1620(+)
Misc Feature(51)1621..1623(+)
Misc Feature(52)1636..1638(+)
Misc Feature(53)1735..1737(+)
Misc Feature(54)1762..1764(+)
Misc Feature(55)1777..1779(+)
Misc Feature(56)1885..1887(+)
Exon (1)1..605
Gene:LMNA
Gene Synonym:
Exon (2)606..762
Gene:LMNA
Gene Synonym:
Exon (3)763..888
Gene:LMNA
Gene Synonym:
Exon (4)889..1059
Gene:LMNA
Gene Synonym:
Exon (5)1060..1185
Gene:LMNA
Gene Synonym:
Exon (6)1186..1406
Gene:LMNA
Gene Synonym:
Exon (7)1407..1629
Gene:LMNA
Gene Synonym:
Exon (8)1630..1737
Gene:LMNA
Gene Synonym:
Exon (9)1738..1857
Gene:LMNA
Gene Synonym:
Exon (10)1858..1947
Gene:LMNA
Gene Synonym:
Exon (11)1948..2067
Gene:LMNA
Gene Synonym:
Exon (12)2068..3077
Gene:LMNA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001282626
Organism Homo sapiens (human)
Definition Homo sapiens lamin A/C (LMNA), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282626

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGAGACCC CGTCCCAGCG GCGCGCCACC CGCAGCGGGG CGCAGGCCAG CTCCACTCCG 
CTGTCGCCCA CCCGCATCAC CCGGCTGCAG GAGAAGGAGG ACCTGCAGGA GCTCAATGAT
CGCTTGGCGG TCTACATCGA CCGTGTGCGC TCGCTGGAAA CGGAGAACGC AGGGCTGCGC
CTTCGCATCA CCGAGTCTGA AGAGGTGGTC AGCCGCGAGG TGTCCGGCAT CAAGGCCGCC
TACGAGGCCG AGCTCGGGGA TGCCCGCAAG ACCCTTGACT CAGTAGCCAA GGAGCGCGCC
CGCCTGCAGC TGGAGCTGAG CAAAGTGCGT GAGGAGTTTA AGGAGCTGAA AGCGCGCAAT
ACCAAGAAGG AGGGTGACCT GATAGCTGCT CAGGCTCGGC TGAAGGACCT GGAGGCTCTG
CTGAACTCCA AGGAGGCCGC ACTGAGCACT GCTCTCAGTG AGAAGCGCAC GCTGGAGGGC
GAGCTGCATG ATCTGCGGGG CCAGGTGGCC AAGCTTGAGG CAGCCCTAGG TGAGGCCAAG
AAGCAACTTC AGGATGAGAT GCTGCGGCGG GTGGATGCTG AGAACAGGCT GCAGACCATG
AAGGAGGAAC TGGACTTCCA GAAGAACATC TACAGTGAGG AGCTGCGTGA GACCAAGCGC
CGTCATGAGA CCCGACTGGT GGAGATTGAC AATGGGAAGC AGCGTGAGTT TGAGAGCCGG
CTGGCGGATG CGCTGCAGGA ACTGCGGGCC CAGCATGAGG ACCAGGTGGA GCAGTATAAG
AAGGAGCTGG AGAAGACTTA TTCTGCCAAG CTGGACAATG CCAGGCAGTC TGCTGAGAGG
AACAGCAACC TGGTGGGGGC TGCCCACGAG GAGCTGCAGC AGTCGCGCAT CCGCATCGAC
AGCCTCTCTG CCCAGCTCAG CCAGCTCCAG AAGCAGCTGG CAGCCAAGGA GGCGAAGCTT
CGAGACCTGG AGGACTCACT GGCCCGTGAG CGGGACACCA GCCGGCGGCT GCTGGCGGAA
AAGGAGCGGG AGATGGCCGA GATGCGGGCA AGGATGCAGC AGCAGCTGGA CGAGTACCAG
GAGCTTCTGG ACATCAAGCT GGCCCTGGAC ATGGAGATCC ACGCCTACCG CAAGCTCTTG
GAGGGCGAGG AGGAGAGGCT ACGCCTGTCC CCCAGCCCTA CCTCGCAGCG CAGCCGTGGC
CGTGCTTCCT CTCACTCATC CCAGACACAG GGTGGGGGCA GCGTCACCAA AAAGCGCAAA
CTGGAGTCCA CTGAGAGCCG CAGCAGCTTC TCACAGCACG CACGCACTAG CGGGCGCGTG
GCCGTGGAGG AGGTGGATGA GGAGGGCAAG TTTGTCCGGC TGCGCAACAA GTCCAATGAG
GACCAGTCCA TGGGCAATTG GCAGATCAAG CGCCAGAATG GAGATGATCC CTTGCTGACT
TACCGGTTCC CACCAAAGTT CACCCTGAAG GCTGGGCAGG TGGTGACGAT CTGGGCTGCA
GGAGCTGGGG CCACCCACAG CCCCCCTACC GACCTGGTGT GGAAGGCACA GAACACCTGG
GGCTGCGGGA ACAGCCTGCG TACGGCTCTC ATCAACTCCA CTGGGGAAGA AGTGGCCATG
CGCAAGCTGG TGCGCTCAGT GACTGTGGTT GAGGACGACG AGGATGAGGA TGGAGATGAC
CTGCTCCATC ACCACCACGG CTCCCACTGC AGCAGCTCGG GGGACCCCGC TGAGTACAAC
CTGCGCTCGC GCACCGTGCT GTGCGGGACC TGCGGGCAGC CTGCCGACAA GGCATCTGCC
AGCGGCTCAG GAGCCCAGAG CCCCCAGAAC TGCAGCATCA TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27626
Clone ID Related Accession (Same CDS sequence) NM_001282624
Accession Version NM_001282624.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1476bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-JUL-2015
Organism Homo sapiens (human)
Product lamin isoform E
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY027676.1, AK097801.1 and BC000511.2. On Sep 17, 2013 this sequence version replaced gi:383792155. Transcript Variant: This variant (5) differs in both UTRs and has multiple differences in the coding region compared to variant 1. This variant encodes isoform E, which is shorter and has distinct N- and C-termini compared to isoform prelamin A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK097801.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCAGCCAC TCCTGTGCTT GGGGAACCTG GAGGATGCAA GGGAAAGGAC TGGCACTCTG 
CTGGCACAGC ACCCGGCCTG GGGCAGGACA CGGGCGAAGC CAGGGTCTCC CCTCAATACC
AAGAAGGAGG GTGACCTGAT AGCTGCTCAG GCTCGGCTGA AGGACCTGGA GGCTCTGCTG
AACTCCAAGG AGGCCGCACT GAGCACTGCT CTCAGTGAGA AGCGCACGCT GGAGGGCGAG
CTGCATGATC TGCGGGGCCA GGTGGCCAAG CTTGAGGCAG CCCTAGGTGA GGCCAAGAAG
CAACTTCAGG ATGAGATGCT GCGGCGGGTG GATGCTGAGA ACAGGCTGCA GACCATGAAG
GAGGAACTGG ACTTCCAGAA GAACATCTAC AGTGAGGAGC TGCGTGAGAC CAAGCGCCGT
CATGAGACCC GACTGGTGGA GATTGACAAT GGGAAGCAGC GTGAGTTTGA GAGCCGGCTG
GCGGATGCGC TGCAGGAACT GCGGGCCCAG CATGAGGACC AGGTGGAGCA GTATAAGAAG
GAGCTGGAGA AGACTTATTC TGCCAAGCTG GACAATGCCA GGCAGTCTGC TGAGAGGAAC
AGCAACCTGG TGGGGGCTGC CCACGAGGAG CTGCAGCAGT CGCGCATCCG CATCGACAGC
CTCTCTGCCC AGCTCAGCCA GCTCCAGAAG CAGCTGGCAG CCAAGGAGGC GAAGCTTCGA
GACCTGGAGG ACTCACTGGC CCGTGAGCGG GACACCAGCC GGCGGCTGCT GGCGGAAAAG
GAGCGGGAGA TGGCCGAGAT GCGGGCAAGG ATGCAGCAGC AGCTGGACGA GTACCAGGAG
CTTCTGGACA TCAAGCTGGC CCTGGACATG GAGATCCACG CCTACCGCAA GCTCTTGGAG
GGCGAGGAGG AGAGGCTACG CCTGTCCCCC AGCCCTACCT CGCAGCGCAG CCGTGGCCGT
GCTTCCTCTC ACTCATCCCA GACACAGGGT GGGGGCAGCG TCACCAAAAA GCGCAAACTG
GAGTCCACTG AGAGCCGCAG CAGCTTCTCA CAGCACGCAC GCACTAGCGG GCGCGTGGCC
GTGGAGGAGG TGGATGAGGA GGGCAAGTTT GTCCGGCTGC GCAACAAGTC CAATGAGGAC
CAGTCCATGG GCAATTGGCA GATCAAGCGC CAGAATGGAG ATGATCCCTT GCTGACTTAC
CGGTTCCCAC CAAAGTTCAC CCTGAAGGCT GGGCAGGTGG TGACGATCTG GGCTGCAGGA
GCTGGGGCCA CCCACAGCCC CCCTACCGAC CTGGTGTGGA AGGCACAGAA CACCTGGGGC
TGCGGGAACA GCCTGCGTAC GGCTCTCATC AACTCCACTG GGGAAGAAGT GGCCATGCGC
AAGCTGGTGC GCTCAGTGAC TGTGGTTGAG GACGACGAGG ATGAGGATGG AGATGACCTG
CTCCATCACC ACCACGTGAG TGGTAGCCGC CGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001269553.1
CDS175..1650
Misc Feature(1)130..132(+)
Misc Feature(2)439..>720(+)
Misc Feature(3)481..1023(+)
Misc Feature(4)835..>1020(+)
Misc Feature(5)1228..1575(+)
Exon (1)1..107
Gene:LMNA
Gene Synonym:
Exon (2)108..287
Gene:LMNA
Gene Synonym:
Exon (3)288..444
Gene:LMNA
Gene Synonym:
Exon (4)445..570
Gene:LMNA
Gene Synonym:
Exon (5)571..741
Gene:LMNA
Gene Synonym:
Exon (6)742..867
Gene:LMNA
Gene Synonym:
Exon (7)868..1088
Gene:LMNA
Gene Synonym:
Exon (8)1089..1311
Gene:LMNA
Gene Synonym:
Exon (9)1312..1419
Gene:LMNA
Gene Synonym:
Exon (10)1420..1539
Gene:LMNA
Gene Synonym:
Exon (11)1540..1752
Gene:LMNA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001282624
Organism Homo sapiens (human)
Definition Homo sapiens lamin A/C (LMNA), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282624

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCAGCCAC TCCTGTGCTT GGGGAACCTG GAGGATGCAA GGGAAAGGAC TGGCACTCTG 
CTGGCACAGC ACCCGGCCTG GGGCAGGACA CGGGCGAAGC CAGGGTCTCC CCTCAATACC
AAGAAGGAGG GTGACCTGAT AGCTGCTCAG GCTCGGCTGA AGGACCTGGA GGCTCTGCTG
AACTCCAAGG AGGCCGCACT GAGCACTGCT CTCAGTGAGA AGCGCACGCT GGAGGGCGAG
CTGCATGATC TGCGGGGCCA GGTGGCCAAG CTTGAGGCAG CCCTAGGTGA GGCCAAGAAG
CAACTTCAGG ATGAGATGCT GCGGCGGGTG GATGCTGAGA ACAGGCTGCA GACCATGAAG
GAGGAACTGG ACTTCCAGAA GAACATCTAC AGTGAGGAGC TGCGTGAGAC CAAGCGCCGT
CATGAGACCC GACTGGTGGA GATTGACAAT GGGAAGCAGC GTGAGTTTGA GAGCCGGCTG
GCGGATGCGC TGCAGGAACT GCGGGCCCAG CATGAGGACC AGGTGGAGCA GTATAAGAAG
GAGCTGGAGA AGACTTATTC TGCCAAGCTG GACAATGCCA GGCAGTCTGC TGAGAGGAAC
AGCAACCTGG TGGGGGCTGC CCACGAGGAG CTGCAGCAGT CGCGCATCCG CATCGACAGC
CTCTCTGCCC AGCTCAGCCA GCTCCAGAAG CAGCTGGCAG CCAAGGAGGC GAAGCTTCGA
GACCTGGAGG ACTCACTGGC CCGTGAGCGG GACACCAGCC GGCGGCTGCT GGCGGAAAAG
GAGCGGGAGA TGGCCGAGAT GCGGGCAAGG ATGCAGCAGC AGCTGGACGA GTACCAGGAG
CTTCTGGACA TCAAGCTGGC CCTGGACATG GAGATCCACG CCTACCGCAA GCTCTTGGAG
GGCGAGGAGG AGAGGCTACG CCTGTCCCCC AGCCCTACCT CGCAGCGCAG CCGTGGCCGT
GCTTCCTCTC ACTCATCCCA GACACAGGGT GGGGGCAGCG TCACCAAAAA GCGCAAACTG
GAGTCCACTG AGAGCCGCAG CAGCTTCTCA CAGCACGCAC GCACTAGCGG GCGCGTGGCC
GTGGAGGAGG TGGATGAGGA GGGCAAGTTT GTCCGGCTGC GCAACAAGTC CAATGAGGAC
CAGTCCATGG GCAATTGGCA GATCAAGCGC CAGAATGGAG ATGATCCCTT GCTGACTTAC
CGGTTCCCAC CAAAGTTCAC CCTGAAGGCT GGGCAGGTGG TGACGATCTG GGCTGCAGGA
GCTGGGGCCA CCCACAGCCC CCCTACCGAC CTGGTGTGGA AGGCACAGAA CACCTGGGGC
TGCGGGAACA GCCTGCGTAC GGCTCTCATC AACTCCACTG GGGAAGAAGT GGCCATGCGC
AAGCTGGTGC GCTCAGTGAC TGTGGTTGAG GACGACGAGG ATGAGGATGG AGATGACCTG
CTCCATCACC ACCACGTGAG TGGTAGCCGC CGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22769
Clone ID Related Accession (Same CDS sequence) NM_001257374
Accession Version NM_001257374.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1725bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-JUL-2015
Organism Homo sapiens (human)
Product lamin isoform D
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY027676.1, AK295390.1, BC018863.2, AL135927.14 and AI872233.1. On Sep 17, 2013 this sequence version replaced gi:383792149. Transcript Variant: This variant (4) represents use of an alternate promoter and uses an alternate 3' exon structure compared to variant 1. The resulting protein (isoform D) has distinct N- and C-termini and is shorter than isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK295390.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2146982 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGGGAACT CTGAGGGCTG CAATACCAAG AAGGAGGGTG ACCTGATAGC TGCTCAGGCT 
CGGCTGAAGG ACCTGGAGGC TCTGCTGAAC TCCAAGGAGG CCGCACTGAG CACTGCTCTC
AGTGAGAAGC GCACGCTGGA GGGCGAGCTG CATGATCTGC GGGGCCAGGT GGCCAAGCTT
GAGGCAGCCC TAGGTGAGGC CAAGAAGCAA CTTCAGGATG AGATGCTGCG GCGGGTGGAT
GCTGAGAACA GGCTGCAGAC CATGAAGGAG GAACTGGACT TCCAGAAGAA CATCTACAGT
GAGGAGCTGC GTGAGACCAA GCGCCGTCAT GAGACCCGAC TGGTGGAGAT TGACAATGGG
AAGCAGCGTG AGTTTGAGAG CCGGCTGGCG GATGCGCTGC AGGAACTGCG GGCCCAGCAT
GAGGACCAGG TGGAGCAGTA TAAGAAGGAG CTGGAGAAGA CTTATTCTGC CAAGCTGGAC
AATGCCAGGC AGTCTGCTGA GAGGAACAGC AACCTGGTGG GGGCTGCCCA CGAGGAGCTG
CAGCAGTCGC GCATCCGCAT CGACAGCCTC TCTGCCCAGC TCAGCCAGCT CCAGAAGCAG
CTGGCAGCCA AGGAGGCGAA GCTTCGAGAC CTGGAGGACT CACTGGCCCG TGAGCGGGAC
ACCAGCCGGC GGCTGCTGGC GGAAAAGGAG CGGGAGATGG CCGAGATGCG GGCAAGGATG
CAGCAGCAGC TGGACGAGTA CCAGGAGCTT CTGGACATCA AGCTGGCCCT GGACATGGAG
ATCCACGCCT ACCGCAAGCT CTTGGAGGGC GAGGAGGAGA GGCTACGCCT GTCCCCCAGC
CCTACCTCGC AGCGCAGCCG TGGCCGTGCT TCCTCTCACT CATCCCAGAC ACAGGGTGGG
GGCAGCGTCA CCAAAAAGCG CAAACTGGAG TCCACTGAGA GCCGCAGCAG CTTCTCACAG
CACGCACGCA CTAGCGGGCG CGTGGCCGTG GAGGAGGTGG ATGAGGAGGG CAAGTTTGTC
CGGCTGCGCA ACAAGTCCAA TGAGGACCAG TCCATGGGCA ATTGGCAGAT CAAGCGCCAG
AATGGAGATG ATCCCTTGCT GACTTACCGG TTCCCACCAA AGTTCACCCT GAAGGCTGGG
CAGGTGGTGA CGATCTGGGC TGCAGGAGCT GGGGCCACCC ACAGCCCCCC TACCGACCTG
GTGTGGAAGG CACAGAACAC CTGGGGCTGC GGGAACAGCC TGCGTACGGC TCTCATCAAC
TCCACTGGGG AAGAAGTGGC CATGCGCAAG CTGGTGCGCT CAGTGACTGT GGTTGAGGAC
GACGAGGATG AGGATGGAGA TGACCTGCTC CATCACCACC ACGGCTCCCA CTGCAGCAGC
TCGGGGGACC CCGCTGAGTA CAACCTGCGC TCGCGCACCG TGCTGTGCGG GACCTGCGGG
CAGCCTGCCG ACAAGGCATC TGCCAGCGGC TCAGGAGCCC AGGTGGGCGG ACCCATCTCC
TCTGGCTCTT CTGCCTCCAG TGTCACGGTC ACTCGCAGCT ACCGCAGTGT GGGGGGCAGT
GGGGGTGGCA GCTTCGGGGA CAATCTGGTC ACCCGCTCCT ACCTCCTGGG CAACTCCAGC
CCCCGAACCC AGAGCCCCCA GAACTGCAGC ATCATACAAG AGATGGGAAT GAGGTGGGAG
GTGGAAGAAG GGAGAAGAAA GGTGAGTTTG AGCTGCCTTC CCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001244303.1
CDS88..1812
Misc Feature(1)259..>540(+)
Misc Feature(2)301..843(+)
Misc Feature(3)655..>840(+)
Misc Feature(4)1048..1395(+)
Exon (1)1..107
Gene:LMNA
Gene Synonym:
Exon (2)108..264
Gene:LMNA
Gene Synonym:
Exon (3)265..390
Gene:LMNA
Gene Synonym:
Exon (4)391..561
Gene:LMNA
Gene Synonym:
Exon (5)562..687
Gene:LMNA
Gene Synonym:
Exon (6)688..908
Gene:LMNA
Gene Synonym:
Exon (7)909..1131
Gene:LMNA
Gene Synonym:
Exon (8)1132..1239
Gene:LMNA
Gene Synonym:
Exon (9)1240..1359
Gene:LMNA
Gene Synonym:
Exon (10)1360..1449
Gene:LMNA
Gene Synonym:
Exon (11)1450..1719
Gene:LMNA
Gene Synonym:
Exon (12)1720..1742
Gene:LMNA
Gene Synonym:
Exon (13)1743..2062
Gene:LMNA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001257374
Organism Homo sapiens (human)
Definition Homo sapiens lamin A/C (LMNA), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001257374

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGGGAACT CTGAGGGCTG CAATACCAAG AAGGAGGGTG ACCTGATAGC TGCTCAGGCT 
CGGCTGAAGG ACCTGGAGGC TCTGCTGAAC TCCAAGGAGG CCGCACTGAG CACTGCTCTC
AGTGAGAAGC GCACGCTGGA GGGCGAGCTG CATGATCTGC GGGGCCAGGT GGCCAAGCTT
GAGGCAGCCC TAGGTGAGGC CAAGAAGCAA CTTCAGGATG AGATGCTGCG GCGGGTGGAT
GCTGAGAACA GGCTGCAGAC CATGAAGGAG GAACTGGACT TCCAGAAGAA CATCTACAGT
GAGGAGCTGC GTGAGACCAA GCGCCGTCAT GAGACCCGAC TGGTGGAGAT TGACAATGGG
AAGCAGCGTG AGTTTGAGAG CCGGCTGGCG GATGCGCTGC AGGAACTGCG GGCCCAGCAT
GAGGACCAGG TGGAGCAGTA TAAGAAGGAG CTGGAGAAGA CTTATTCTGC CAAGCTGGAC
AATGCCAGGC AGTCTGCTGA GAGGAACAGC AACCTGGTGG GGGCTGCCCA CGAGGAGCTG
CAGCAGTCGC GCATCCGCAT CGACAGCCTC TCTGCCCAGC TCAGCCAGCT CCAGAAGCAG
CTGGCAGCCA AGGAGGCGAA GCTTCGAGAC CTGGAGGACT CACTGGCCCG TGAGCGGGAC
ACCAGCCGGC GGCTGCTGGC GGAAAAGGAG CGGGAGATGG CCGAGATGCG GGCAAGGATG
CAGCAGCAGC TGGACGAGTA CCAGGAGCTT CTGGACATCA AGCTGGCCCT GGACATGGAG
ATCCACGCCT ACCGCAAGCT CTTGGAGGGC GAGGAGGAGA GGCTACGCCT GTCCCCCAGC
CCTACCTCGC AGCGCAGCCG TGGCCGTGCT TCCTCTCACT CATCCCAGAC ACAGGGTGGG
GGCAGCGTCA CCAAAAAGCG CAAACTGGAG TCCACTGAGA GCCGCAGCAG CTTCTCACAG
CACGCACGCA CTAGCGGGCG CGTGGCCGTG GAGGAGGTGG ATGAGGAGGG CAAGTTTGTC
CGGCTGCGCA ACAAGTCCAA TGAGGACCAG TCCATGGGCA ATTGGCAGAT CAAGCGCCAG
AATGGAGATG ATCCCTTGCT GACTTACCGG TTCCCACCAA AGTTCACCCT GAAGGCTGGG
CAGGTGGTGA CGATCTGGGC TGCAGGAGCT GGGGCCACCC ACAGCCCCCC TACCGACCTG
GTGTGGAAGG CACAGAACAC CTGGGGCTGC GGGAACAGCC TGCGTACGGC TCTCATCAAC
TCCACTGGGG AAGAAGTGGC CATGCGCAAG CTGGTGCGCT CAGTGACTGT GGTTGAGGAC
GACGAGGATG AGGATGGAGA TGACCTGCTC CATCACCACC ACGGCTCCCA CTGCAGCAGC
TCGGGGGACC CCGCTGAGTA CAACCTGCGC TCGCGCACCG TGCTGTGCGG GACCTGCGGG
CAGCCTGCCG ACAAGGCATC TGCCAGCGGC TCAGGAGCCC AGGTGGGCGG ACCCATCTCC
TCTGGCTCTT CTGCCTCCAG TGTCACGGTC ACTCGCAGCT ACCGCAGTGT GGGGGGCAGT
GGGGGTGGCA GCTTCGGGGA CAATCTGGTC ACCCGCTCCT ACCTCCTGGG CAACTCCAGC
CCCCGAACCC AGAGCCCCCA GAACTGCAGC ATCATACAAG AGATGGGAAT GAGGTGGGAG
GTGGAAGAAG GGAGAAGAAA GGTGAGTTTG AGCTGCCTTC CCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20696
Clone ID Related Accession (Same CDS sequence) NM_170708
Accession Version NM_170708.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1905bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-JUL-2015
Organism Homo sapiens (human)
Product lamin isoform A-delta10
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG822820.1, BC000511.2, AF381029.1, BC014507.1, AL135927.14 and AI872233.1. On Apr 10, 2012 this sequence version replaced gi:153281094. Transcript Variant: This variant (3) lacks an internal segment of sequence compared to variant 1. The encoded isoform (A delta10), is shorter but has the same C-terminus when compared to isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGAGACCC CGTCCCAGCG GCGCGCCACC CGCAGCGGGG CGCAGGCCAG CTCCACTCCG 
CTGTCGCCCA CCCGCATCAC CCGGCTGCAG GAGAAGGAGG ACCTGCAGGA GCTCAATGAT
CGCTTGGCGG TCTACATCGA CCGTGTGCGC TCGCTGGAAA CGGAGAACGC AGGGCTGCGC
CTTCGCATCA CCGAGTCTGA AGAGGTGGTC AGCCGCGAGG TGTCCGGCAT CAAGGCCGCC
TACGAGGCCG AGCTCGGGGA TGCCCGCAAG ACCCTTGACT CAGTAGCCAA GGAGCGCGCC
CGCCTGCAGC TGGAGCTGAG CAAAGTGCGT GAGGAGTTTA AGGAGCTGAA AGCGCGCAAT
ACCAAGAAGG AGGGTGACCT GATAGCTGCT CAGGCTCGGC TGAAGGACCT GGAGGCTCTG
CTGAACTCCA AGGAGGCCGC ACTGAGCACT GCTCTCAGTG AGAAGCGCAC GCTGGAGGGC
GAGCTGCATG ATCTGCGGGG CCAGGTGGCC AAGCTTGAGG CAGCCCTAGG TGAGGCCAAG
AAGCAACTTC AGGATGAGAT GCTGCGGCGG GTGGATGCTG AGAACAGGCT GCAGACCATG
AAGGAGGAAC TGGACTTCCA GAAGAACATC TACAGTGAGG AGCTGCGTGA GACCAAGCGC
CGTCATGAGA CCCGACTGGT GGAGATTGAC AATGGGAAGC AGCGTGAGTT TGAGAGCCGG
CTGGCGGATG CGCTGCAGGA ACTGCGGGCC CAGCATGAGG ACCAGGTGGA GCAGTATAAG
AAGGAGCTGG AGAAGACTTA TTCTGCCAAG CTGGACAATG CCAGGCAGTC TGCTGAGAGG
AACAGCAACC TGGTGGGGGC TGCCCACGAG GAGCTGCAGC AGTCGCGCAT CCGCATCGAC
AGCCTCTCTG CCCAGCTCAG CCAGCTCCAG AAGCAGCTGG CAGCCAAGGA GGCGAAGCTT
CGAGACCTGG AGGACTCACT GGCCCGTGAG CGGGACACCA GCCGGCGGCT GCTGGCGGAA
AAGGAGCGGG AGATGGCCGA GATGCGGGCA AGGATGCAGC AGCAGCTGGA CGAGTACCAG
GAGCTTCTGG ACATCAAGCT GGCCCTGGAC ATGGAGATCC ACGCCTACCG CAAGCTCTTG
GAGGGCGAGG AGGAGAGGCT ACGCCTGTCC CCCAGCCCTA CCTCGCAGCG CAGCCGTGGC
CGTGCTTCCT CTCACTCATC CCAGACACAG GGTGGGGGCA GCGTCACCAA AAAGCGCAAA
CTGGAGTCCA CTGAGAGCCG CAGCAGCTTC TCACAGCACG CACGCACTAG CGGGCGCGTG
GCCGTGGAGG AGGTGGATGA GGAGGGCAAG TTTGTCCGGC TGCGCAACAA GTCCAATGAG
GACCAGTCCA TGGGCAATTG GCAGATCAAG CGCCAGAATG GAGATGATCC CTTGCTGACT
TACCGGTTCC CACCAAAGTT CACCCTGAAG GCTGGGCAGG TGGTGACGAT CTGGGCTGCA
GGAGCTGGGG CCACCCACAG CCCCCCTACC GACCTGGTGT GGAAGGCACA GAACACCTGG
GGCTGCGGGA ACAGCCTGCG TACGGCTCTC ATCAACTCCA CTGGGGAAGG CTCCCACTGC
AGCAGCTCGG GGGACCCCGC TGAGTACAAC CTGCGCTCGC GCACCGTGCT GTGCGGGACC
TGCGGGCAGC CTGCCGACAA GGCATCTGCC AGCGGCTCAG GAGCCCAGGT GGGCGGACCC
ATCTCCTCTG GCTCTTCTGC CTCCAGTGTC ACGGTCACTC GCAGCTACCG CAGTGTGGGG
GGCAGTGGGG GTGGCAGCTT CGGGGACAAT CTGGTCACCC GCTCCTACCT CCTGGGCAAC
TCCAGCCCCC GAACCCAGAG CCCCCAGAAC TGCAGCATCA TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_733822.1
CDS250..2154
Misc Feature(1)13..15(+)
Misc Feature(2)277..279(+)
Misc Feature(3)304..306(+)
Misc Feature(4)304..306(+)
Misc Feature(5)304..306(+)
Misc Feature(6)313..315(+)
Misc Feature(7)313..315(+)
Misc Feature(8)319..321(+)
Misc Feature(9)337..1407(+)
Misc Feature(10)343..693(+)
Misc Feature(11)529..531(+)
Misc Feature(12)757..>1038(+)
Misc Feature(13)937..939(+)
Misc Feature(14)937..939(+)
Misc Feature(15)1078..1080(+)
Misc Feature(16)1093..1095(+)
Misc Feature(17)1153..>1338(+)
Misc Feature(18)1417..1419(+)
Misc Feature(19)1417..1419(+)
Misc Feature(20)1423..1425(+)
Misc Feature(21)1423..1425(+)
Misc Feature(22)1423..1425(+)
Misc Feature(23)1429..1431(+)
Misc Feature(24)1432..1434(+)
Misc Feature(25)1456..1458(+)
Misc Feature(26)1459..1461(+)
Misc Feature(27)1465..1467(+)
Misc Feature(28)1468..1470(+)
Misc Feature(29)1474..1476(+)
Misc Feature(30)1489..1491(+)
Misc Feature(31)1495..1497(+)
Misc Feature(32)1516..1518(+)
Misc Feature(33)1519..1521(+)
Misc Feature(34)1525..1527(+)
Misc Feature(35)1546..1857(+)
Misc Feature(36)1822..1824(+)
Misc Feature(37)1993..1995(+)
Misc Feature(38)1996..1998(+)
Misc Feature(39)2005..2007(+)
Misc Feature(40)2011..2013(+)
Misc Feature(41)2014..2016(+)
Misc Feature(42)2041..2043(+)
Misc Feature(43)2053..2055(+)
Misc Feature(44)2065..2067(+)
Misc Feature(45)2086..2088(+)
Misc Feature(46)2095..2097(+)
Misc Feature(47)2113..2115(+)
Misc Feature(48)2113..2115(+)
Exon (1)1..605
Gene:LMNA
Gene Synonym:
Exon (2)606..762
Gene:LMNA
Gene Synonym:
Exon (3)763..888
Gene:LMNA
Gene Synonym:
Exon (4)889..1059
Gene:LMNA
Gene Synonym:
Exon (5)1060..1185
Gene:LMNA
Gene Synonym:
Exon (6)1186..1406
Gene:LMNA
Gene Synonym:
Exon (7)1407..1629
Gene:LMNA
Gene Synonym:
Exon (8)1630..1737
Gene:LMNA
Gene Synonym:
Exon (9)1738..1857
Gene:LMNA
Gene Synonym:
Exon (10)1858..2127
Gene:LMNA
Gene Synonym:
Exon (11)2128..3137
Gene:LMNA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_170708
Organism Homo sapiens (human)
Definition Homo sapiens lamin A/C (LMNA), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_170708

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGAGACCC CGTCCCAGCG GCGCGCCACC CGCAGCGGGG CGCAGGCCAG CTCCACTCCG 
CTGTCGCCCA CCCGCATCAC CCGGCTGCAG GAGAAGGAGG ACCTGCAGGA GCTCAATGAT
CGCTTGGCGG TCTACATCGA CCGTGTGCGC TCGCTGGAAA CGGAGAACGC AGGGCTGCGC
CTTCGCATCA CCGAGTCTGA AGAGGTGGTC AGCCGCGAGG TGTCCGGCAT CAAGGCCGCC
TACGAGGCCG AGCTCGGGGA TGCCCGCAAG ACCCTTGACT CAGTAGCCAA GGAGCGCGCC
CGCCTGCAGC TGGAGCTGAG CAAAGTGCGT GAGGAGTTTA AGGAGCTGAA AGCGCGCAAT
ACCAAGAAGG AGGGTGACCT GATAGCTGCT CAGGCTCGGC TGAAGGACCT GGAGGCTCTG
CTGAACTCCA AGGAGGCCGC ACTGAGCACT GCTCTCAGTG AGAAGCGCAC GCTGGAGGGC
GAGCTGCATG ATCTGCGGGG CCAGGTGGCC AAGCTTGAGG CAGCCCTAGG TGAGGCCAAG
AAGCAACTTC AGGATGAGAT GCTGCGGCGG GTGGATGCTG AGAACAGGCT GCAGACCATG
AAGGAGGAAC TGGACTTCCA GAAGAACATC TACAGTGAGG AGCTGCGTGA GACCAAGCGC
CGTCATGAGA CCCGACTGGT GGAGATTGAC AATGGGAAGC AGCGTGAGTT TGAGAGCCGG
CTGGCGGATG CGCTGCAGGA ACTGCGGGCC CAGCATGAGG ACCAGGTGGA GCAGTATAAG
AAGGAGCTGG AGAAGACTTA TTCTGCCAAG CTGGACAATG CCAGGCAGTC TGCTGAGAGG
AACAGCAACC TGGTGGGGGC TGCCCACGAG GAGCTGCAGC AGTCGCGCAT CCGCATCGAC
AGCCTCTCTG CCCAGCTCAG CCAGCTCCAG AAGCAGCTGG CAGCCAAGGA GGCGAAGCTT
CGAGACCTGG AGGACTCACT GGCCCGTGAG CGGGACACCA GCCGGCGGCT GCTGGCGGAA
AAGGAGCGGG AGATGGCCGA GATGCGGGCA AGGATGCAGC AGCAGCTGGA CGAGTACCAG
GAGCTTCTGG ACATCAAGCT GGCCCTGGAC ATGGAGATCC ACGCCTACCG CAAGCTCTTG
GAGGGCGAGG AGGAGAGGCT ACGCCTGTCC CCCAGCCCTA CCTCGCAGCG CAGCCGTGGC
CGTGCTTCCT CTCACTCATC CCAGACACAG GGTGGGGGCA GCGTCACCAA AAAGCGCAAA
CTGGAGTCCA CTGAGAGCCG CAGCAGCTTC TCACAGCACG CACGCACTAG CGGGCGCGTG
GCCGTGGAGG AGGTGGATGA GGAGGGCAAG TTTGTCCGGC TGCGCAACAA GTCCAATGAG
GACCAGTCCA TGGGCAATTG GCAGATCAAG CGCCAGAATG GAGATGATCC CTTGCTGACT
TACCGGTTCC CACCAAAGTT CACCCTGAAG GCTGGGCAGG TGGTGACGAT CTGGGCTGCA
GGAGCTGGGG CCACCCACAG CCCCCCTACC GACCTGGTGT GGAAGGCACA GAACACCTGG
GGCTGCGGGA ACAGCCTGCG TACGGCTCTC ATCAACTCCA CTGGGGAAGG CTCCCACTGC
AGCAGCTCGG GGGACCCCGC TGAGTACAAC CTGCGCTCGC GCACCGTGCT GTGCGGGACC
TGCGGGCAGC CTGCCGACAA GGCATCTGCC AGCGGCTCAG GAGCCCAGGT GGGCGGACCC
ATCTCCTCTG GCTCTTCTGC CTCCAGTGTC ACGGTCACTC GCAGCTACCG CAGTGTGGGG
GGCAGTGGGG GTGGCAGCTT CGGGGACAAT CTGGTCACCC GCTCCTACCT CCTGGGCAAC
TCCAGCCCCC GAACCCAGAG CCCCCAGAAC TGCAGCATCA TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16808
Clone ID Related Accession (Same CDS sequence) NM_001282625 , NM_005572
Accession Version NM_005572.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1719bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-JUL-2015
Organism Homo sapiens (human)
Product lamin isoform C
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA421696.1, X03445.1 and AA558657.1. This sequence is a reference standard in the RefSeqGene project. On Jul 24, 2007 this sequence version replaced gi:27436944. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1. This results in a shorter isoform (C) with a distinct C-terminus when compared to isoform prelamin A. Both variants 2 and 6 encode the same isoform (C). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000511.2, X03445.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGAGACCC CGTCCCAGCG GCGCGCCACC CGCAGCGGGG CGCAGGCCAG CTCCACTCCG 
CTGTCGCCCA CCCGCATCAC CCGGCTGCAG GAGAAGGAGG ACCTGCAGGA GCTCAATGAT
CGCTTGGCGG TCTACATCGA CCGTGTGCGC TCGCTGGAAA CGGAGAACGC AGGGCTGCGC
CTTCGCATCA CCGAGTCTGA AGAGGTGGTC AGCCGCGAGG TGTCCGGCAT CAAGGCCGCC
TACGAGGCCG AGCTCGGGGA TGCCCGCAAG ACCCTTGACT CAGTAGCCAA GGAGCGCGCC
CGCCTGCAGC TGGAGCTGAG CAAAGTGCGT GAGGAGTTTA AGGAGCTGAA AGCGCGCAAT
ACCAAGAAGG AGGGTGACCT GATAGCTGCT CAGGCTCGGC TGAAGGACCT GGAGGCTCTG
CTGAACTCCA AGGAGGCCGC ACTGAGCACT GCTCTCAGTG AGAAGCGCAC GCTGGAGGGC
GAGCTGCATG ATCTGCGGGG CCAGGTGGCC AAGCTTGAGG CAGCCCTAGG TGAGGCCAAG
AAGCAACTTC AGGATGAGAT GCTGCGGCGG GTGGATGCTG AGAACAGGCT GCAGACCATG
AAGGAGGAAC TGGACTTCCA GAAGAACATC TACAGTGAGG AGCTGCGTGA GACCAAGCGC
CGTCATGAGA CCCGACTGGT GGAGATTGAC AATGGGAAGC AGCGTGAGTT TGAGAGCCGG
CTGGCGGATG CGCTGCAGGA ACTGCGGGCC CAGCATGAGG ACCAGGTGGA GCAGTATAAG
AAGGAGCTGG AGAAGACTTA TTCTGCCAAG CTGGACAATG CCAGGCAGTC TGCTGAGAGG
AACAGCAACC TGGTGGGGGC TGCCCACGAG GAGCTGCAGC AGTCGCGCAT CCGCATCGAC
AGCCTCTCTG CCCAGCTCAG CCAGCTCCAG AAGCAGCTGG CAGCCAAGGA GGCGAAGCTT
CGAGACCTGG AGGACTCACT GGCCCGTGAG CGGGACACCA GCCGGCGGCT GCTGGCGGAA
AAGGAGCGGG AGATGGCCGA GATGCGGGCA AGGATGCAGC AGCAGCTGGA CGAGTACCAG
GAGCTTCTGG ACATCAAGCT GGCCCTGGAC ATGGAGATCC ACGCCTACCG CAAGCTCTTG
GAGGGCGAGG AGGAGAGGCT ACGCCTGTCC CCCAGCCCTA CCTCGCAGCG CAGCCGTGGC
CGTGCTTCCT CTCACTCATC CCAGACACAG GGTGGGGGCA GCGTCACCAA AAAGCGCAAA
CTGGAGTCCA CTGAGAGCCG CAGCAGCTTC TCACAGCACG CACGCACTAG CGGGCGCGTG
GCCGTGGAGG AGGTGGATGA GGAGGGCAAG TTTGTCCGGC TGCGCAACAA GTCCAATGAG
GACCAGTCCA TGGGCAATTG GCAGATCAAG CGCCAGAATG GAGATGATCC CTTGCTGACT
TACCGGTTCC CACCAAAGTT CACCCTGAAG GCTGGGCAGG TGGTGACGAT CTGGGCTGCA
GGAGCTGGGG CCACCCACAG CCCCCCTACC GACCTGGTGT GGAAGGCACA GAACACCTGG
GGCTGCGGGA ACAGCCTGCG TACGGCTCTC ATCAACTCCA CTGGGGAAGA AGTGGCCATG
CGCAAGCTGG TGCGCTCAGT GACTGTGGTT GAGGACGACG AGGATGAGGA TGGAGATGAC
CTGCTCCATC ACCACCACGT GAGTGGTAGC CGCCGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005563.1
CDS250..1968
Misc Feature(1)13..15(+)
Misc Feature(2)250..639(+)
Misc Feature(3)250..348(+)
Misc Feature(4)250..252(+)
Misc Feature(5)256..258(+)
Misc Feature(6)277..279(+)
Misc Feature(7)283..285(+)
Misc Feature(8)301..303(+)
Misc Feature(9)304..306(+)
Misc Feature(10)304..306(+)
Misc Feature(11)304..306(+)
Misc Feature(12)304..306(+)
Misc Feature(13)313..315(+)
Misc Feature(14)313..315(+)
Misc Feature(15)313..315(+)
Misc Feature(16)319..321(+)
Misc Feature(17)337..1407(+)
Misc Feature(18)343..345(+)
Misc Feature(19)343..345(+)
Misc Feature(20)349..1398(+)
Misc Feature(21)349..459(+)
Misc Feature(22)460..489(+)
Misc Feature(23)490..903(+)
Misc Feature(24)529..531(+)
Misc Feature(25)571..573(+)
Misc Feature(26)616..618(+)
Misc Feature(27)652..654(+)
Misc Feature(28)712..714(+)
Misc Feature(29)757..>1038(+)
Misc Feature(30)760..762(+)
Misc Feature(31)760..762(+)
Misc Feature(32)850..852(+)
Misc Feature(33)883..885(+)
Misc Feature(34)904..975(+)
Misc Feature(35)937..939(+)
Misc Feature(36)937..939(+)
Misc Feature(37)976..1398(+)
Misc Feature(38)1027..1029(+)
Misc Feature(39)1045..1047(+)
Misc Feature(40)1057..1059(+)
Misc Feature(41)1078..1080(+)
Misc Feature(42)1078..1080(+)
Misc Feature(43)1093..1095(+)
Misc Feature(44)1150..1152(+)
Misc Feature(45)1153..>1338(+)
Misc Feature(46)1168..1170(+)
Misc Feature(47)1180..1182(+)
Misc Feature(48)1222..1224(+)
Misc Feature(49)1237..1239(+)
Misc Feature(50)1417..1419(+)
Misc Feature(51)1417..1419(+)
Misc Feature(52)1417..1419(+)
Misc Feature(53)1423..1425(+)
Misc Feature(54)1423..1425(+)
Misc Feature(55)1423..1425(+)
Misc Feature(56)1423..1425(+)
Misc Feature(57)1429..1431(+)
Misc Feature(58)1432..1434(+)
Misc Feature(59)1432..1434(+)
Misc Feature(60)1456..1458(+)
Misc Feature(61)1456..1458(+)
Misc Feature(62)1459..1461(+)
Misc Feature(63)1459..1461(+)
Misc Feature(64)1465..1467(+)
Misc Feature(65)1468..1470(+)
Misc Feature(66)1468..1470(+)
Misc Feature(67)1474..1476(+)
Misc Feature(68)1489..1491(+)
Misc Feature(69)1489..1491(+)
Misc Feature(70)1495..1497(+)
Misc Feature(71)1498..1515(+)
Misc Feature(72)1516..1518(+)
Misc Feature(73)1519..1521(+)
Misc Feature(74)1525..1527(+)
Misc Feature(75)1540..1542(+)
Misc Feature(76)1546..1893(+)
Misc Feature(77)1597..1599(+)
Misc Feature(78)1618..1620(+)
Misc Feature(79)1621..1623(+)
Misc Feature(80)1636..1638(+)
Misc Feature(81)1735..1737(+)
Misc Feature(82)1762..1764(+)
Misc Feature(83)1777..1779(+)
Misc Feature(84)1822..1824(+)
Misc Feature(85)1885..1887(+)
Exon (1)1..605
Gene:LMNA
Gene Synonym:
Exon (2)606..762
Gene:LMNA
Gene Synonym:
Exon (3)763..888
Gene:LMNA
Gene Synonym:
Exon (4)889..1059
Gene:LMNA
Gene Synonym:
Exon (5)1060..1185
Gene:LMNA
Gene Synonym:
Exon (6)1186..1406
Gene:LMNA
Gene Synonym:
Exon (7)1407..1629
Gene:LMNA
Gene Synonym:
Exon (8)1630..1737
Gene:LMNA
Gene Synonym:
Exon (9)1738..1857
Gene:LMNA
Gene Synonym:
Exon (10)1858..2070
Gene:LMNA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_005572
Organism Homo sapiens (human)
Definition Homo sapiens lamin A/C (LMNA), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005572

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGAGACCC CGTCCCAGCG GCGCGCCACC CGCAGCGGGG CGCAGGCCAG CTCCACTCCG 
CTGTCGCCCA CCCGCATCAC CCGGCTGCAG GAGAAGGAGG ACCTGCAGGA GCTCAATGAT
CGCTTGGCGG TCTACATCGA CCGTGTGCGC TCGCTGGAAA CGGAGAACGC AGGGCTGCGC
CTTCGCATCA CCGAGTCTGA AGAGGTGGTC AGCCGCGAGG TGTCCGGCAT CAAGGCCGCC
TACGAGGCCG AGCTCGGGGA TGCCCGCAAG ACCCTTGACT CAGTAGCCAA GGAGCGCGCC
CGCCTGCAGC TGGAGCTGAG CAAAGTGCGT GAGGAGTTTA AGGAGCTGAA AGCGCGCAAT
ACCAAGAAGG AGGGTGACCT GATAGCTGCT CAGGCTCGGC TGAAGGACCT GGAGGCTCTG
CTGAACTCCA AGGAGGCCGC ACTGAGCACT GCTCTCAGTG AGAAGCGCAC GCTGGAGGGC
GAGCTGCATG ATCTGCGGGG CCAGGTGGCC AAGCTTGAGG CAGCCCTAGG TGAGGCCAAG
AAGCAACTTC AGGATGAGAT GCTGCGGCGG GTGGATGCTG AGAACAGGCT GCAGACCATG
AAGGAGGAAC TGGACTTCCA GAAGAACATC TACAGTGAGG AGCTGCGTGA GACCAAGCGC
CGTCATGAGA CCCGACTGGT GGAGATTGAC AATGGGAAGC AGCGTGAGTT TGAGAGCCGG
CTGGCGGATG CGCTGCAGGA ACTGCGGGCC CAGCATGAGG ACCAGGTGGA GCAGTATAAG
AAGGAGCTGG AGAAGACTTA TTCTGCCAAG CTGGACAATG CCAGGCAGTC TGCTGAGAGG
AACAGCAACC TGGTGGGGGC TGCCCACGAG GAGCTGCAGC AGTCGCGCAT CCGCATCGAC
AGCCTCTCTG CCCAGCTCAG CCAGCTCCAG AAGCAGCTGG CAGCCAAGGA GGCGAAGCTT
CGAGACCTGG AGGACTCACT GGCCCGTGAG CGGGACACCA GCCGGCGGCT GCTGGCGGAA
AAGGAGCGGG AGATGGCCGA GATGCGGGCA AGGATGCAGC AGCAGCTGGA CGAGTACCAG
GAGCTTCTGG ACATCAAGCT GGCCCTGGAC ATGGAGATCC ACGCCTACCG CAAGCTCTTG
GAGGGCGAGG AGGAGAGGCT ACGCCTGTCC CCCAGCCCTA CCTCGCAGCG CAGCCGTGGC
CGTGCTTCCT CTCACTCATC CCAGACACAG GGTGGGGGCA GCGTCACCAA AAAGCGCAAA
CTGGAGTCCA CTGAGAGCCG CAGCAGCTTC TCACAGCACG CACGCACTAG CGGGCGCGTG
GCCGTGGAGG AGGTGGATGA GGAGGGCAAG TTTGTCCGGC TGCGCAACAA GTCCAATGAG
GACCAGTCCA TGGGCAATTG GCAGATCAAG CGCCAGAATG GAGATGATCC CTTGCTGACT
TACCGGTTCC CACCAAAGTT CACCCTGAAG GCTGGGCAGG TGGTGACGAT CTGGGCTGCA
GGAGCTGGGG CCACCCACAG CCCCCCTACC GACCTGGTGT GGAAGGCACA GAACACCTGG
GGCTGCGGGA ACAGCCTGCG TACGGCTCTC ATCAACTCCA CTGGGGAAGA AGTGGCCATG
CGCAAGCTGG TGCGCTCAGT GACTGTGGTT GAGGACGACG AGGATGAGGA TGGAGATGAC
CTGCTCCATC ACCACCACGT GAGTGGTAGC CGCCGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16808
Clone ID Related Accession (Same CDS sequence) NM_001282625 , NM_005572
Accession Version NM_001282625.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1719bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-JUL-2015
Organism Homo sapiens (human)
Product lamin isoform C
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB270595.1, AK056143.1 and BC000511.2. On Sep 17, 2013 this sequence version replaced gi:383792152. Transcript Variant: This variant (6) differs in both UTRs and has multiple differences in the coding region compared to variant 1. This results in a shorter isoform (C) with a distinct C-terminus when compared to isoform prelamin A. Both variants 2 and 6 encode the same isoform (C). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK056143.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA2156099 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGAGACCC CGTCCCAGCG GCGCGCCACC CGCAGCGGGG CGCAGGCCAG CTCCACTCCG 
CTGTCGCCCA CCCGCATCAC CCGGCTGCAG GAGAAGGAGG ACCTGCAGGA GCTCAATGAT
CGCTTGGCGG TCTACATCGA CCGTGTGCGC TCGCTGGAAA CGGAGAACGC AGGGCTGCGC
CTTCGCATCA CCGAGTCTGA AGAGGTGGTC AGCCGCGAGG TGTCCGGCAT CAAGGCCGCC
TACGAGGCCG AGCTCGGGGA TGCCCGCAAG ACCCTTGACT CAGTAGCCAA GGAGCGCGCC
CGCCTGCAGC TGGAGCTGAG CAAAGTGCGT GAGGAGTTTA AGGAGCTGAA AGCGCGCAAT
ACCAAGAAGG AGGGTGACCT GATAGCTGCT CAGGCTCGGC TGAAGGACCT GGAGGCTCTG
CTGAACTCCA AGGAGGCCGC ACTGAGCACT GCTCTCAGTG AGAAGCGCAC GCTGGAGGGC
GAGCTGCATG ATCTGCGGGG CCAGGTGGCC AAGCTTGAGG CAGCCCTAGG TGAGGCCAAG
AAGCAACTTC AGGATGAGAT GCTGCGGCGG GTGGATGCTG AGAACAGGCT GCAGACCATG
AAGGAGGAAC TGGACTTCCA GAAGAACATC TACAGTGAGG AGCTGCGTGA GACCAAGCGC
CGTCATGAGA CCCGACTGGT GGAGATTGAC AATGGGAAGC AGCGTGAGTT TGAGAGCCGG
CTGGCGGATG CGCTGCAGGA ACTGCGGGCC CAGCATGAGG ACCAGGTGGA GCAGTATAAG
AAGGAGCTGG AGAAGACTTA TTCTGCCAAG CTGGACAATG CCAGGCAGTC TGCTGAGAGG
AACAGCAACC TGGTGGGGGC TGCCCACGAG GAGCTGCAGC AGTCGCGCAT CCGCATCGAC
AGCCTCTCTG CCCAGCTCAG CCAGCTCCAG AAGCAGCTGG CAGCCAAGGA GGCGAAGCTT
CGAGACCTGG AGGACTCACT GGCCCGTGAG CGGGACACCA GCCGGCGGCT GCTGGCGGAA
AAGGAGCGGG AGATGGCCGA GATGCGGGCA AGGATGCAGC AGCAGCTGGA CGAGTACCAG
GAGCTTCTGG ACATCAAGCT GGCCCTGGAC ATGGAGATCC ACGCCTACCG CAAGCTCTTG
GAGGGCGAGG AGGAGAGGCT ACGCCTGTCC CCCAGCCCTA CCTCGCAGCG CAGCCGTGGC
CGTGCTTCCT CTCACTCATC CCAGACACAG GGTGGGGGCA GCGTCACCAA AAAGCGCAAA
CTGGAGTCCA CTGAGAGCCG CAGCAGCTTC TCACAGCACG CACGCACTAG CGGGCGCGTG
GCCGTGGAGG AGGTGGATGA GGAGGGCAAG TTTGTCCGGC TGCGCAACAA GTCCAATGAG
GACCAGTCCA TGGGCAATTG GCAGATCAAG CGCCAGAATG GAGATGATCC CTTGCTGACT
TACCGGTTCC CACCAAAGTT CACCCTGAAG GCTGGGCAGG TGGTGACGAT CTGGGCTGCA
GGAGCTGGGG CCACCCACAG CCCCCCTACC GACCTGGTGT GGAAGGCACA GAACACCTGG
GGCTGCGGGA ACAGCCTGCG TACGGCTCTC ATCAACTCCA CTGGGGAAGA AGTGGCCATG
CGCAAGCTGG TGCGCTCAGT GACTGTGGTT GAGGACGACG AGGATGAGGA TGGAGATGAC
CTGCTCCATC ACCACCACGT GAGTGGTAGC CGCCGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001269554.1
CDS674..2392
Misc Feature(1)440..442(+)
Misc Feature(2)674..1063(+)
Misc Feature(3)674..772(+)
Misc Feature(4)674..676(+)
Misc Feature(5)680..682(+)
Misc Feature(6)707..709(+)
Misc Feature(7)725..727(+)
Misc Feature(8)728..730(+)
Misc Feature(9)737..739(+)
Misc Feature(10)761..1831(+)
Misc Feature(11)767..769(+)
Misc Feature(12)767..769(+)
Misc Feature(13)773..1822(+)
Misc Feature(14)773..883(+)
Misc Feature(15)884..913(+)
Misc Feature(16)914..1327(+)
Misc Feature(17)995..997(+)
Misc Feature(18)1040..1042(+)
Misc Feature(19)1076..1078(+)
Misc Feature(20)1136..1138(+)
Misc Feature(21)1181..>1462(+)
Misc Feature(22)1184..1186(+)
Misc Feature(23)1184..1186(+)
Misc Feature(24)1274..1276(+)
Misc Feature(25)1307..1309(+)
Misc Feature(26)1328..1399(+)
Misc Feature(27)1400..1822(+)
Misc Feature(28)1451..1453(+)
Misc Feature(29)1469..1471(+)
Misc Feature(30)1481..1483(+)
Misc Feature(31)1502..1504(+)
Misc Feature(32)1574..1576(+)
Misc Feature(33)1577..>1762(+)
Misc Feature(34)1592..1594(+)
Misc Feature(35)1604..1606(+)
Misc Feature(36)1646..1648(+)
Misc Feature(37)1661..1663(+)
Misc Feature(38)1841..1843(+)
Misc Feature(39)1847..1849(+)
Misc Feature(40)1856..1858(+)
Misc Feature(41)1880..1882(+)
Misc Feature(42)1883..1885(+)
Misc Feature(43)1892..1894(+)
Misc Feature(44)1913..1915(+)
Misc Feature(45)1922..1939(+)
Misc Feature(46)1964..1966(+)
Misc Feature(47)1970..2317(+)
Misc Feature(48)2021..2023(+)
Misc Feature(49)2042..2044(+)
Misc Feature(50)2045..2047(+)
Misc Feature(51)2060..2062(+)
Misc Feature(52)2159..2161(+)
Misc Feature(53)2186..2188(+)
Misc Feature(54)2201..2203(+)
Misc Feature(55)2309..2311(+)
Exon (1)1..118
Gene:LMNA
Gene Synonym:
Exon (2)119..355
Gene:LMNA
Gene Synonym:
Exon (3)356..467
Gene:LMNA
Gene Synonym:
Exon (4)468..1029
Gene:LMNA
Gene Synonym:
Exon (5)1030..1186
Gene:LMNA
Gene Synonym:
Exon (6)1187..1312
Gene:LMNA
Gene Synonym:
Exon (7)1313..1483
Gene:LMNA
Gene Synonym:
Exon (8)1484..1609
Gene:LMNA
Gene Synonym:
Exon (9)1610..1830
Gene:LMNA
Gene Synonym:
Exon (10)1831..2053
Gene:LMNA
Gene Synonym:
Exon (11)2054..2161
Gene:LMNA
Gene Synonym:
Exon (12)2162..2281
Gene:LMNA
Gene Synonym:
Exon (13)2282..2494
Gene:LMNA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001282625
Organism Homo sapiens (human)
Definition Homo sapiens lamin A/C (LMNA), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282625

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGAGACCC CGTCCCAGCG GCGCGCCACC CGCAGCGGGG CGCAGGCCAG CTCCACTCCG 
CTGTCGCCCA CCCGCATCAC CCGGCTGCAG GAGAAGGAGG ACCTGCAGGA GCTCAATGAT
CGCTTGGCGG TCTACATCGA CCGTGTGCGC TCGCTGGAAA CGGAGAACGC AGGGCTGCGC
CTTCGCATCA CCGAGTCTGA AGAGGTGGTC AGCCGCGAGG TGTCCGGCAT CAAGGCCGCC
TACGAGGCCG AGCTCGGGGA TGCCCGCAAG ACCCTTGACT CAGTAGCCAA GGAGCGCGCC
CGCCTGCAGC TGGAGCTGAG CAAAGTGCGT GAGGAGTTTA AGGAGCTGAA AGCGCGCAAT
ACCAAGAAGG AGGGTGACCT GATAGCTGCT CAGGCTCGGC TGAAGGACCT GGAGGCTCTG
CTGAACTCCA AGGAGGCCGC ACTGAGCACT GCTCTCAGTG AGAAGCGCAC GCTGGAGGGC
GAGCTGCATG ATCTGCGGGG CCAGGTGGCC AAGCTTGAGG CAGCCCTAGG TGAGGCCAAG
AAGCAACTTC AGGATGAGAT GCTGCGGCGG GTGGATGCTG AGAACAGGCT GCAGACCATG
AAGGAGGAAC TGGACTTCCA GAAGAACATC TACAGTGAGG AGCTGCGTGA GACCAAGCGC
CGTCATGAGA CCCGACTGGT GGAGATTGAC AATGGGAAGC AGCGTGAGTT TGAGAGCCGG
CTGGCGGATG CGCTGCAGGA ACTGCGGGCC CAGCATGAGG ACCAGGTGGA GCAGTATAAG
AAGGAGCTGG AGAAGACTTA TTCTGCCAAG CTGGACAATG CCAGGCAGTC TGCTGAGAGG
AACAGCAACC TGGTGGGGGC TGCCCACGAG GAGCTGCAGC AGTCGCGCAT CCGCATCGAC
AGCCTCTCTG CCCAGCTCAG CCAGCTCCAG AAGCAGCTGG CAGCCAAGGA GGCGAAGCTT
CGAGACCTGG AGGACTCACT GGCCCGTGAG CGGGACACCA GCCGGCGGCT GCTGGCGGAA
AAGGAGCGGG AGATGGCCGA GATGCGGGCA AGGATGCAGC AGCAGCTGGA CGAGTACCAG
GAGCTTCTGG ACATCAAGCT GGCCCTGGAC ATGGAGATCC ACGCCTACCG CAAGCTCTTG
GAGGGCGAGG AGGAGAGGCT ACGCCTGTCC CCCAGCCCTA CCTCGCAGCG CAGCCGTGGC
CGTGCTTCCT CTCACTCATC CCAGACACAG GGTGGGGGCA GCGTCACCAA AAAGCGCAAA
CTGGAGTCCA CTGAGAGCCG CAGCAGCTTC TCACAGCACG CACGCACTAG CGGGCGCGTG
GCCGTGGAGG AGGTGGATGA GGAGGGCAAG TTTGTCCGGC TGCGCAACAA GTCCAATGAG
GACCAGTCCA TGGGCAATTG GCAGATCAAG CGCCAGAATG GAGATGATCC CTTGCTGACT
TACCGGTTCC CACCAAAGTT CACCCTGAAG GCTGGGCAGG TGGTGACGAT CTGGGCTGCA
GGAGCTGGGG CCACCCACAG CCCCCCTACC GACCTGGTGT GGAAGGCACA GAACACCTGG
GGCTGCGGGA ACAGCCTGCG TACGGCTCTC ATCAACTCCA CTGGGGAAGA AGTGGCCATG
CGCAAGCTGG TGCGCTCAGT GACTGTGGTT GAGGACGACG AGGATGAGGA TGGAGATGAC
CTGCTCCATC ACCACCACGT GAGTGGTAGC CGCCGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21250
Clone ID Related Accession (Same CDS sequence) NM_170707
Accession Version NM_170707.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1995bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-JUL-2015
Organism Homo sapiens (human)
Product lamin isoform A
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG822820.1, BC014507.1, AL135927.14 and AI872233.1. On Apr 10, 2012 this sequence version replaced gi:153281093. Transcript Variant: This variant (1) encodes isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK026584.1, BC014507.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGAGACCC CGTCCCAGCG GCGCGCCACC CGCAGCGGGG CGCAGGCCAG CTCCACTCCG 
CTGTCGCCCA CCCGCATCAC CCGGCTGCAG GAGAAGGAGG ACCTGCAGGA GCTCAATGAT
CGCTTGGCGG TCTACATCGA CCGTGTGCGC TCGCTGGAAA CGGAGAACGC AGGGCTGCGC
CTTCGCATCA CCGAGTCTGA AGAGGTGGTC AGCCGCGAGG TGTCCGGCAT CAAGGCCGCC
TACGAGGCCG AGCTCGGGGA TGCCCGCAAG ACCCTTGACT CAGTAGCCAA GGAGCGCGCC
CGCCTGCAGC TGGAGCTGAG CAAAGTGCGT GAGGAGTTTA AGGAGCTGAA AGCGCGCAAT
ACCAAGAAGG AGGGTGACCT GATAGCTGCT CAGGCTCGGC TGAAGGACCT GGAGGCTCTG
CTGAACTCCA AGGAGGCCGC ACTGAGCACT GCTCTCAGTG AGAAGCGCAC GCTGGAGGGC
GAGCTGCATG ATCTGCGGGG CCAGGTGGCC AAGCTTGAGG CAGCCCTAGG TGAGGCCAAG
AAGCAACTTC AGGATGAGAT GCTGCGGCGG GTGGATGCTG AGAACAGGCT GCAGACCATG
AAGGAGGAAC TGGACTTCCA GAAGAACATC TACAGTGAGG AGCTGCGTGA GACCAAGCGC
CGTCATGAGA CCCGACTGGT GGAGATTGAC AATGGGAAGC AGCGTGAGTT TGAGAGCCGG
CTGGCGGATG CGCTGCAGGA ACTGCGGGCC CAGCATGAGG ACCAGGTGGA GCAGTATAAG
AAGGAGCTGG AGAAGACTTA TTCTGCCAAG CTGGACAATG CCAGGCAGTC TGCTGAGAGG
AACAGCAACC TGGTGGGGGC TGCCCACGAG GAGCTGCAGC AGTCGCGCAT CCGCATCGAC
AGCCTCTCTG CCCAGCTCAG CCAGCTCCAG AAGCAGCTGG CAGCCAAGGA GGCGAAGCTT
CGAGACCTGG AGGACTCACT GGCCCGTGAG CGGGACACCA GCCGGCGGCT GCTGGCGGAA
AAGGAGCGGG AGATGGCCGA GATGCGGGCA AGGATGCAGC AGCAGCTGGA CGAGTACCAG
GAGCTTCTGG ACATCAAGCT GGCCCTGGAC ATGGAGATCC ACGCCTACCG CAAGCTCTTG
GAGGGCGAGG AGGAGAGGCT ACGCCTGTCC CCCAGCCCTA CCTCGCAGCG CAGCCGTGGC
CGTGCTTCCT CTCACTCATC CCAGACACAG GGTGGGGGCA GCGTCACCAA AAAGCGCAAA
CTGGAGTCCA CTGAGAGCCG CAGCAGCTTC TCACAGCACG CACGCACTAG CGGGCGCGTG
GCCGTGGAGG AGGTGGATGA GGAGGGCAAG TTTGTCCGGC TGCGCAACAA GTCCAATGAG
GACCAGTCCA TGGGCAATTG GCAGATCAAG CGCCAGAATG GAGATGATCC CTTGCTGACT
TACCGGTTCC CACCAAAGTT CACCCTGAAG GCTGGGCAGG TGGTGACGAT CTGGGCTGCA
GGAGCTGGGG CCACCCACAG CCCCCCTACC GACCTGGTGT GGAAGGCACA GAACACCTGG
GGCTGCGGGA ACAGCCTGCG TACGGCTCTC ATCAACTCCA CTGGGGAAGA AGTGGCCATG
CGCAAGCTGG TGCGCTCAGT GACTGTGGTT GAGGACGACG AGGATGAGGA TGGAGATGAC
CTGCTCCATC ACCACCACGG CTCCCACTGC AGCAGCTCGG GGGACCCCGC TGAGTACAAC
CTGCGCTCGC GCACCGTGCT GTGCGGGACC TGCGGGCAGC CTGCCGACAA GGCATCTGCC
AGCGGCTCAG GAGCCCAGGT GGGCGGACCC ATCTCCTCTG GCTCTTCTGC CTCCAGTGTC
ACGGTCACTC GCAGCTACCG CAGTGTGGGG GGCAGTGGGG GTGGCAGCTT CGGGGACAAT
CTGGTCACCC GCTCCTACCT CCTGGGCAAC TCCAGCCCCC GAACCCAGAG CCCCCAGAAC
TGCAGCATCA TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_733821.1
CDS250..2244
Misc Feature(1)13..15(+)
Misc Feature(2)250..639(+)
Misc Feature(3)250..348(+)
Misc Feature(4)250..252(+)
Misc Feature(5)256..258(+)
Misc Feature(6)277..279(+)
Misc Feature(7)283..285(+)
Misc Feature(8)301..303(+)
Misc Feature(9)304..306(+)
Misc Feature(10)304..306(+)
Misc Feature(11)304..306(+)
Misc Feature(12)304..306(+)
Misc Feature(13)313..315(+)
Misc Feature(14)313..315(+)
Misc Feature(15)313..315(+)
Misc Feature(16)319..321(+)
Misc Feature(17)337..1407(+)
Misc Feature(18)343..693(+)
Misc Feature(19)343..345(+)
Misc Feature(20)343..345(+)
Misc Feature(21)349..1398(+)
Misc Feature(22)349..459(+)
Misc Feature(23)460..489(+)
Misc Feature(24)490..903(+)
Misc Feature(25)529..531(+)
Misc Feature(26)571..573(+)
Misc Feature(27)616..618(+)
Misc Feature(28)652..654(+)
Misc Feature(29)712..714(+)
Misc Feature(30)757..>1038(+)
Misc Feature(31)760..762(+)
Misc Feature(32)760..762(+)
Misc Feature(33)850..852(+)
Misc Feature(34)883..885(+)
Misc Feature(35)904..975(+)
Misc Feature(36)937..939(+)
Misc Feature(37)937..939(+)
Misc Feature(38)976..1398(+)
Misc Feature(39)1027..1029(+)
Misc Feature(40)1045..1047(+)
Misc Feature(41)1057..1059(+)
Misc Feature(42)1078..1080(+)
Misc Feature(43)1078..1080(+)
Misc Feature(44)1093..1095(+)
Misc Feature(45)1150..1152(+)
Misc Feature(46)1153..>1338(+)
Misc Feature(47)1168..1170(+)
Misc Feature(48)1180..1182(+)
Misc Feature(49)1222..1224(+)
Misc Feature(50)1237..1239(+)
Misc Feature(51)1399..2241(+)
Misc Feature(52)1417..1419(+)
Misc Feature(53)1417..1419(+)
Misc Feature(54)1417..1419(+)
Misc Feature(55)1423..1425(+)
Misc Feature(56)1423..1425(+)
Misc Feature(57)1423..1425(+)
Misc Feature(58)1423..1425(+)
Misc Feature(59)1429..1431(+)
Misc Feature(60)1432..1434(+)
Misc Feature(61)1432..1434(+)
Misc Feature(62)1456..1458(+)
Misc Feature(63)1456..1458(+)
Misc Feature(64)1459..1461(+)
Misc Feature(65)1459..1461(+)
Misc Feature(66)1465..1467(+)
Misc Feature(67)1468..1470(+)
Misc Feature(68)1468..1470(+)
Misc Feature(69)1474..1476(+)
Misc Feature(70)1489..1491(+)
Misc Feature(71)1489..1491(+)
Misc Feature(72)1495..1497(+)
Misc Feature(73)1498..1515(+)
Misc Feature(74)1516..1518(+)
Misc Feature(75)1519..1521(+)
Misc Feature(76)1525..1527(+)
Misc Feature(77)1540..1542(+)
Misc Feature(78)1546..1893(+)
Misc Feature(79)1597..1599(+)
Misc Feature(80)1618..1620(+)
Misc Feature(81)1621..1623(+)
Misc Feature(82)1636..1638(+)
Misc Feature(83)1735..1737(+)
Misc Feature(84)1762..1764(+)
Misc Feature(85)1777..1779(+)
Misc Feature(86)1822..1824(+)
Misc Feature(87)1885..1887(+)
Misc Feature(88)2083..2085(+)
Misc Feature(89)2083..2085(+)
Misc Feature(90)2086..2088(+)
Misc Feature(91)2095..2097(+)
Misc Feature(92)2101..2103(+)
Misc Feature(93)2104..2106(+)
Misc Feature(94)2131..2133(+)
Misc Feature(95)2131..2133(+)
Misc Feature(96)2143..2145(+)
Misc Feature(97)2143..2145(+)
Misc Feature(98)2155..2157(+)
Misc Feature(99)2155..2157(+)
Misc Feature(100)2176..2178(+)
Misc Feature(101)2185..2190(+)
Misc Feature(102)2185..2187(+)
Misc Feature(103)2203..2205(+)
Misc Feature(104)2203..2205(+)
Misc Feature(105)2203..2205(+)
Misc Feature(106)2230..2232(+)
Exon (1)1..605
Gene:LMNA
Gene Synonym:
Exon (2)606..762
Gene:LMNA
Gene Synonym:
Exon (3)763..888
Gene:LMNA
Gene Synonym:
Exon (4)889..1059
Gene:LMNA
Gene Synonym:
Exon (5)1060..1185
Gene:LMNA
Gene Synonym:
Exon (6)1186..1406
Gene:LMNA
Gene Synonym:
Exon (7)1407..1629
Gene:LMNA
Gene Synonym:
Exon (8)1630..1737
Gene:LMNA
Gene Synonym:
Exon (9)1738..1857
Gene:LMNA
Gene Synonym:
Exon (10)1858..1947
Gene:LMNA
Gene Synonym:
Exon (11)1948..2217
Gene:LMNA
Gene Synonym:
Exon (12)2218..3227
Gene:LMNA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_170707
Organism Homo sapiens (human)
Definition Homo sapiens lamin A/C (LMNA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_170707

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGAGACCC CGTCCCAGCG GCGCGCCACC CGCAGCGGGG CGCAGGCCAG CTCCACTCCG 
CTGTCGCCCA CCCGCATCAC CCGGCTGCAG GAGAAGGAGG ACCTGCAGGA GCTCAATGAT
CGCTTGGCGG TCTACATCGA CCGTGTGCGC TCGCTGGAAA CGGAGAACGC AGGGCTGCGC
CTTCGCATCA CCGAGTCTGA AGAGGTGGTC AGCCGCGAGG TGTCCGGCAT CAAGGCCGCC
TACGAGGCCG AGCTCGGGGA TGCCCGCAAG ACCCTTGACT CAGTAGCCAA GGAGCGCGCC
CGCCTGCAGC TGGAGCTGAG CAAAGTGCGT GAGGAGTTTA AGGAGCTGAA AGCGCGCAAT
ACCAAGAAGG AGGGTGACCT GATAGCTGCT CAGGCTCGGC TGAAGGACCT GGAGGCTCTG
CTGAACTCCA AGGAGGCCGC ACTGAGCACT GCTCTCAGTG AGAAGCGCAC GCTGGAGGGC
GAGCTGCATG ATCTGCGGGG CCAGGTGGCC AAGCTTGAGG CAGCCCTAGG TGAGGCCAAG
AAGCAACTTC AGGATGAGAT GCTGCGGCGG GTGGATGCTG AGAACAGGCT GCAGACCATG
AAGGAGGAAC TGGACTTCCA GAAGAACATC TACAGTGAGG AGCTGCGTGA GACCAAGCGC
CGTCATGAGA CCCGACTGGT GGAGATTGAC AATGGGAAGC AGCGTGAGTT TGAGAGCCGG
CTGGCGGATG CGCTGCAGGA ACTGCGGGCC CAGCATGAGG ACCAGGTGGA GCAGTATAAG
AAGGAGCTGG AGAAGACTTA TTCTGCCAAG CTGGACAATG CCAGGCAGTC TGCTGAGAGG
AACAGCAACC TGGTGGGGGC TGCCCACGAG GAGCTGCAGC AGTCGCGCAT CCGCATCGAC
AGCCTCTCTG CCCAGCTCAG CCAGCTCCAG AAGCAGCTGG CAGCCAAGGA GGCGAAGCTT
CGAGACCTGG AGGACTCACT GGCCCGTGAG CGGGACACCA GCCGGCGGCT GCTGGCGGAA
AAGGAGCGGG AGATGGCCGA GATGCGGGCA AGGATGCAGC AGCAGCTGGA CGAGTACCAG
GAGCTTCTGG ACATCAAGCT GGCCCTGGAC ATGGAGATCC ACGCCTACCG CAAGCTCTTG
GAGGGCGAGG AGGAGAGGCT ACGCCTGTCC CCCAGCCCTA CCTCGCAGCG CAGCCGTGGC
CGTGCTTCCT CTCACTCATC CCAGACACAG GGTGGGGGCA GCGTCACCAA AAAGCGCAAA
CTGGAGTCCA CTGAGAGCCG CAGCAGCTTC TCACAGCACG CACGCACTAG CGGGCGCGTG
GCCGTGGAGG AGGTGGATGA GGAGGGCAAG TTTGTCCGGC TGCGCAACAA GTCCAATGAG
GACCAGTCCA TGGGCAATTG GCAGATCAAG CGCCAGAATG GAGATGATCC CTTGCTGACT
TACCGGTTCC CACCAAAGTT CACCCTGAAG GCTGGGCAGG TGGTGACGAT CTGGGCTGCA
GGAGCTGGGG CCACCCACAG CCCCCCTACC GACCTGGTGT GGAAGGCACA GAACACCTGG
GGCTGCGGGA ACAGCCTGCG TACGGCTCTC ATCAACTCCA CTGGGGAAGA AGTGGCCATG
CGCAAGCTGG TGCGCTCAGT GACTGTGGTT GAGGACGACG AGGATGAGGA TGGAGATGAC
CTGCTCCATC ACCACCACGG CTCCCACTGC AGCAGCTCGG GGGACCCCGC TGAGTACAAC
CTGCGCTCGC GCACCGTGCT GTGCGGGACC TGCGGGCAGC CTGCCGACAA GGCATCTGCC
AGCGGCTCAG GAGCCCAGGT GGGCGGACCC ATCTCCTCTG GCTCTTCTGC CTCCAGTGTC
ACGGTCACTC GCAGCTACCG CAGTGTGGGG GGCAGTGGGG GTGGCAGCTT CGGGGACAAT
CTGGTCACCC GCTCCTACCT CCTGGGCAAC TCCAGCCCCC GAACCCAGAG CCCCCAGAAC
TGCAGCATCA TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58735
Clone ID Related Accession (Same CDS sequence) XM_011509533
Accession Version XM_011509533.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1659bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lamin isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGGGAACT CTGAGGGCTG CAATACCAAG AAGGAGGGTG ACCTGATAGC TGCTCAGGCT 
CGGCTGAAGG ACCTGGAGGC TCTGCTGAAC TCCAAGGAGG CCGCACTGAG CACTGCTCTC
AGTGAGAAGC GCACGCTGGA GGGCGAGCTG CATGATCTGC GGGGCCAGGT GGCCAAGCTT
GAGGCAGCCC TAGGTGAGGC CAAGAAGCAA CTTCAGGATG AGATGCTGCG GCGGGTGGAT
GCTGAGAACA GGCTGCAGAC CATGAAGGAG GAACTGGACT TCCAGAAGAA CATCTACAGT
GAGGAGCTGC GTGAGACCAA GCGCCGTCAT GAGACCCGAC TGGTGGAGAT TGACAATGGG
AAGCAGCGTG AGTTTGAGAG CCGGCTGGCG GATGCGCTGC AGGAACTGCG GGCCCAGCAT
GAGGACCAGG TGGAGCAGTA TAAGAAGGAG CTGGAGAAGA CTTATTCTGC CAAGCTGGAC
AATGCCAGGC AGTCTGCTGA GAGGAACAGC AACCTGGTGG GGGCTGCCCA CGAGGAGCTG
CAGCAGTCGC GCATCCGCAT CGACAGCCTC TCTGCCCAGC TCAGCCAGCT CCAGAAGCAG
CTGGCAGCCA AGGAGGCGAA GCTTCGAGAC CTGGAGGACT CACTGGCCCG TGAGCGGGAC
ACCAGCCGGC GGCTGCTGGC GGAAAAGGAG CGGGAGATGG CCGAGATGCG GGCAAGGATG
CAGCAGCAGC TGGACGAGTA CCAGGAGCTT CTGGACATCA AGCTGGCCCT GGACATGGAG
ATCCACGCCT ACCGCAAGCT CTTGGAGGGC GAGGAGGAGA GGCTACGCCT GTCCCCCAGC
CCTACCTCGC AGCGCAGCCG TGGCCGTGCT TCCTCTCACT CATCCCAGAC ACAGGGTGGG
GGCAGCGTCA CCAAAAAGCG CAAACTGGAG TCCACTGAGA GCCGCAGCAG CTTCTCACAG
CACGCACGCA CTAGCGGGCG CGTGGCCGTG GAGGAGGTGG ATGAGGAGGG CAAGTTTGTC
CGGCTGCGCA ACAAGTCCAA TGAGGACCAG TCCATGGGCA ATTGGCAGAT CAAGCGCCAG
AATGGAGATG ATCCCTTGCT GACTTACCGG TTCCCACCAA AGTTCACCCT GAAGGCTGGG
CAGGTGGTGA CGATCTGGGC TGCAGGAGCT GGGGCCACCC ACAGCCCCCC TACCGACCTG
GTGTGGAAGG CACAGAACAC CTGGGGCTGC GGGAACAGCC TGCGTACGGC TCTCATCAAC
TCCACTGGGG AAGAAGTGGC CATGCGCAAG CTGGTGCGCT CAGTGACTGT GGTTGAGGAC
GACGAGGATG AGGATGGAGA TGACCTGCTC CATCACCACC ACGGCTCCCA CTGCAGCAGC
TCGGGGGACC CCGCTGAGTA CAACCTGCGC TCGCGCACCG TGCTGTGCGG GACCTGCGGG
CAGCCTGCCG ACAAGGCATC TGCCAGCGGC TCAGGAGCCC AGGTGGGCGG ACCCATCTCC
TCTGGCTCTT CTGCCTCCAG TGTCACGGTC ACTCGCAGCT ACCGCAGTGT GGGGGGCAGT
GGGGGTGGCA GCTTCGGGGA CAATCTGGTC ACCCGCTCCT ACCTCCTGGG CAACTCCAGC
CCCCGAACCC AGAGCCCCCA GAACTGCAGC ATCATGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011507835.1
CDS83..1741
Misc Feature(1)254..>535(+)
Misc Feature(2)296..838(+)
Misc Feature(3)650..>835(+)
Misc Feature(4)1043..1390(+)
Translation

Target ORF information:

RefSeq Version XM_011509533
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lamin A/C (LMNA), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509533

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGGGAACT CTGAGGGCTG CAATACCAAG AAGGAGGGTG ACCTGATAGC TGCTCAGGCT 
CGGCTGAAGG ACCTGGAGGC TCTGCTGAAC TCCAAGGAGG CCGCACTGAG CACTGCTCTC
AGTGAGAAGC GCACGCTGGA GGGCGAGCTG CATGATCTGC GGGGCCAGGT GGCCAAGCTT
GAGGCAGCCC TAGGTGAGGC CAAGAAGCAA CTTCAGGATG AGATGCTGCG GCGGGTGGAT
GCTGAGAACA GGCTGCAGAC CATGAAGGAG GAACTGGACT TCCAGAAGAA CATCTACAGT
GAGGAGCTGC GTGAGACCAA GCGCCGTCAT GAGACCCGAC TGGTGGAGAT TGACAATGGG
AAGCAGCGTG AGTTTGAGAG CCGGCTGGCG GATGCGCTGC AGGAACTGCG GGCCCAGCAT
GAGGACCAGG TGGAGCAGTA TAAGAAGGAG CTGGAGAAGA CTTATTCTGC CAAGCTGGAC
AATGCCAGGC AGTCTGCTGA GAGGAACAGC AACCTGGTGG GGGCTGCCCA CGAGGAGCTG
CAGCAGTCGC GCATCCGCAT CGACAGCCTC TCTGCCCAGC TCAGCCAGCT CCAGAAGCAG
CTGGCAGCCA AGGAGGCGAA GCTTCGAGAC CTGGAGGACT CACTGGCCCG TGAGCGGGAC
ACCAGCCGGC GGCTGCTGGC GGAAAAGGAG CGGGAGATGG CCGAGATGCG GGCAAGGATG
CAGCAGCAGC TGGACGAGTA CCAGGAGCTT CTGGACATCA AGCTGGCCCT GGACATGGAG
ATCCACGCCT ACCGCAAGCT CTTGGAGGGC GAGGAGGAGA GGCTACGCCT GTCCCCCAGC
CCTACCTCGC AGCGCAGCCG TGGCCGTGCT TCCTCTCACT CATCCCAGAC ACAGGGTGGG
GGCAGCGTCA CCAAAAAGCG CAAACTGGAG TCCACTGAGA GCCGCAGCAG CTTCTCACAG
CACGCACGCA CTAGCGGGCG CGTGGCCGTG GAGGAGGTGG ATGAGGAGGG CAAGTTTGTC
CGGCTGCGCA ACAAGTCCAA TGAGGACCAG TCCATGGGCA ATTGGCAGAT CAAGCGCCAG
AATGGAGATG ATCCCTTGCT GACTTACCGG TTCCCACCAA AGTTCACCCT GAAGGCTGGG
CAGGTGGTGA CGATCTGGGC TGCAGGAGCT GGGGCCACCC ACAGCCCCCC TACCGACCTG
GTGTGGAAGG CACAGAACAC CTGGGGCTGC GGGAACAGCC TGCGTACGGC TCTCATCAAC
TCCACTGGGG AAGAAGTGGC CATGCGCAAG CTGGTGCGCT CAGTGACTGT GGTTGAGGAC
GACGAGGATG AGGATGGAGA TGACCTGCTC CATCACCACC ACGGCTCCCA CTGCAGCAGC
TCGGGGGACC CCGCTGAGTA CAACCTGCGC TCGCGCACCG TGCTGTGCGG GACCTGCGGG
CAGCCTGCCG ACAAGGCATC TGCCAGCGGC TCAGGAGCCC AGGTGGGCGG ACCCATCTCC
TCTGGCTCTT CTGCCTCCAG TGTCACGGTC ACTCGCAGCT ACCGCAGTGT GGGGGGCAGT
GGGGGTGGCA GCTTCGGGGA CAATCTGGTC ACCCGCTCCT ACCTCCTGGG CAACTCCAGC
CCCCGAACCC AGAGCCCCCA GAACTGCAGC ATCATGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58736
Clone ID Related Accession (Same CDS sequence) XM_011509534
Accession Version XM_011509534.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1371bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lamin isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGACCCG ACTGGTGGAG ATTGACAATG GGAAGCAGCG TGAGTTTGAG AGCCGGCTGG 
CGGATGCGCT GCAGGAACTG CGGGCCCAGC ATGAGGACCA GGTGGAGCAG TATAAGAAGG
AGCTGGAGAA GACTTATTCT GCCAAGCTGG ACAATGCCAG GCAGTCTGCT GAGAGGAACA
GCAACCTGGT GGGGGCTGCC CACGAGGAGC TGCAGCAGTC GCGCATCCGC ATCGACAGCC
TCTCTGCCCA GCTCAGCCAG CTCCAGAAGC AGGGCCCTTG GGAGCTCACC AAACCCTCCC
ACCCCCCTTC AGCTGGCAGC CAAGGAGGCG AAGCTTCGAG ACCTGGAGGA CTCACTGGCC
CGTGAGCGGG ACACCAGCCG GCGGCTGCTG GCGGAAAAGG AGCGGGAGAT GGCCGAGATG
CGGGCAAGGA TGCAGCAGCA GCTGGACGAG TACCAGGAGC TTCTGGACAT CAAGCTGGCC
CTGGACATGG AGATCCACGC CTACCGCAAG CTCTTGGAGG GCGAGGAGGA GAGGCTACGC
CTGTCCCCCA GCCCTACCTC GCAGCGCAGC CGTGGCCGTG CTTCCTCTCA CTCATCCCAG
ACACAGGGTG GGGGCAGCGT CACCAAAAAG CGCAAACTGG AGTCCACTGA GAGCCGCAGC
AGCTTCTCAC AGCACGCACG CACTAGCGGG CGCGTGGCCG TGGAGGAGGT GGATGAGGAG
GGCAAGTTTG TCCGGCTGCG CAACAAGTCC AATGAGGACC AGTCCATGGG CAATTGGCAG
ATCAAGCGCC AGAATGGAGA TGATCCCTTG CTGACTTACC GGTTCCCACC AAAGTTCACC
CTGAAGGCTG GGCAGGTGGT GACGATCTGG GCTGCAGGAG CTGGGGCCAC CCACAGCCCC
CCTACCGACC TGGTGTGGAA GGCACAGAAC ACCTGGGGCT GCGGGAACAG CCTGCGTACG
GCTCTCATCA ACTCCACTGG GGAAGAAGTG GCCATGCGCA AGCTGGTGCG CTCAGTGACT
GTGGTTGAGG ACGACGAGGA TGAGGATGGA GATGACCTGC TCCATCACCA CCACGGCTCC
CACTGCAGCA GCTCGGGGGA CCCCGCTGAG TACAACCTGC GCTCGCGCAC CGTGCTGTGC
GGGACCTGCG GGCAGCCTGC CGACAAGGCA TCTGCCAGCG GCTCAGGAGC CCAGGTGGGC
GGACCCATCT CCTCTGGCTC TTCTGCCTCC AGTGTCACGG TCACTCGCAG CTACCGCAGT
GTGGGGGGCA GTGGGGGTGG CAGCTTCGGG GACAATCTGG TCACCCGCTC CTACCTCCTG
GGCAACTCCA GCCCCCGAAC CCAGAGCCCC CAGAACTGCA GCATCATGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011507836.1
CDS309..1679
Misc Feature(1)618..>773(+)
Misc Feature(2)633..>989(+)
Misc Feature(3)981..1328(+)
Translation

Target ORF information:

RefSeq Version XM_011509534
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lamin A/C (LMNA), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509534

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGACCCG ACTGGTGGAG ATTGACAATG GGAAGCAGCG TGAGTTTGAG AGCCGGCTGG 
CGGATGCGCT GCAGGAACTG CGGGCCCAGC ATGAGGACCA GGTGGAGCAG TATAAGAAGG
AGCTGGAGAA GACTTATTCT GCCAAGCTGG ACAATGCCAG GCAGTCTGCT GAGAGGAACA
GCAACCTGGT GGGGGCTGCC CACGAGGAGC TGCAGCAGTC GCGCATCCGC ATCGACAGCC
TCTCTGCCCA GCTCAGCCAG CTCCAGAAGC AGGGCCCTTG GGAGCTCACC AAACCCTCCC
ACCCCCCTTC AGCTGGCAGC CAAGGAGGCG AAGCTTCGAG ACCTGGAGGA CTCACTGGCC
CGTGAGCGGG ACACCAGCCG GCGGCTGCTG GCGGAAAAGG AGCGGGAGAT GGCCGAGATG
CGGGCAAGGA TGCAGCAGCA GCTGGACGAG TACCAGGAGC TTCTGGACAT CAAGCTGGCC
CTGGACATGG AGATCCACGC CTACCGCAAG CTCTTGGAGG GCGAGGAGGA GAGGCTACGC
CTGTCCCCCA GCCCTACCTC GCAGCGCAGC CGTGGCCGTG CTTCCTCTCA CTCATCCCAG
ACACAGGGTG GGGGCAGCGT CACCAAAAAG CGCAAACTGG AGTCCACTGA GAGCCGCAGC
AGCTTCTCAC AGCACGCACG CACTAGCGGG CGCGTGGCCG TGGAGGAGGT GGATGAGGAG
GGCAAGTTTG TCCGGCTGCG CAACAAGTCC AATGAGGACC AGTCCATGGG CAATTGGCAG
ATCAAGCGCC AGAATGGAGA TGATCCCTTG CTGACTTACC GGTTCCCACC AAAGTTCACC
CTGAAGGCTG GGCAGGTGGT GACGATCTGG GCTGCAGGAG CTGGGGCCAC CCACAGCCCC
CCTACCGACC TGGTGTGGAA GGCACAGAAC ACCTGGGGCT GCGGGAACAG CCTGCGTACG
GCTCTCATCA ACTCCACTGG GGAAGAAGTG GCCATGCGCA AGCTGGTGCG CTCAGTGACT
GTGGTTGAGG ACGACGAGGA TGAGGATGGA GATGACCTGC TCCATCACCA CCACGGCTCC
CACTGCAGCA GCTCGGGGGA CCCCGCTGAG TACAACCTGC GCTCGCGCAC CGTGCTGTGC
GGGACCTGCG GGCAGCCTGC CGACAAGGCA TCTGCCAGCG GCTCAGGAGC CCAGGTGGGC
GGACCCATCT CCTCTGGCTC TTCTGCCTCC AGTGTCACGG TCACTCGCAG CTACCGCAGT
GTGGGGGGCA GTGGGGGTGG CAGCTTCGGG GACAATCTGG TCACCCGCTC CTACCTCCTG
GGCAACTCCA GCCCCCGAAC CCAGAGCCCC CAGAACTGCA GCATCATGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

The mutant form of lamin A that causes Hutchinson-Gilford progeria is a biomarker of cellular aging in human skin
PLoS ONE 2 (12), E1269 (2007)
McClintock D, Ratner D, Lokuge M, Owens DM, Gordon LB, Collins FS and Djabali K.


book

Lamin A-dependent nuclear defects in human aging
Science 312 (5776), 1059-1063 (2006)
Scaffidi P and Misteli T.


book

Lamin a truncation in Hutchinson-Gilford progeria
Science 300 (5628), 2055 (2003)
De Sandre-Giovannoli A, Bernard R, Cau P, Navarro C, Amiel J, Boccaccio I, Lyonnet S, Stewart CL, Munnich A, Le Merrer M and Levy N.


book

LMNA-Related Dilated Cardiomyopathy
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Hershberger,R.E. and Morales,A.


book

Emery-Dreifuss Muscular Dystrophy
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Bonne,G., Leturcq,F. and Ben Yaou,R.


book

Limb-Girdle Muscular Dystrophy Overview
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Pegoraro,E. and Hoffman,E.P.


book

Charcot-Marie-Tooth Hereditary Neuropathy Overview
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Bird,T.D.


book

Dilated Cardiomyopathy Overview
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Hershberger,R.E. and Morales,A.


book

Charcot-Marie-Tooth Neuropathy Type 2
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Bird,T.D.


book

Hutchinson-Gilford Progeria Syndrome
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Gordon,L.B., Brown,W.T. and Collins,F.S.


 
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