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GTF2H5 cDNA ORF clone, Homo sapiens (human)

Gene Symbol GTF2H5
Entrez Gene ID 404672
Full Name general transcription factor IIH, polypeptide 5
Synonyms C6orf175, TFB5, TFIIH, TGF2H5, TTD, TTD-A, TTD3, TTDA, bA120J8.2
General protein information
Preferred Names
general transcription factor IIH subunit 5
Names
general transcription factor IIH subunit 5
TFB5 ortholog
TFIIH basal transcription factor complex TTDA subunit
TFIIH basal transcription factor complex TTD-A subunit
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6q25.3

Summary This gene encodes a subunit of transcription/repair factor TFIIH, which functions in gene transcription and DNA repair. This protein stimulates ERCC3/XPB ATPase activity to trigger DNA opening during DNA repair, and is implicated in regulating cellular levels of TFIIH. Mutations in this gene result in trichothiodystrophy, complementation group A. [provided by RefSeq, Mar 2009]. lac of sum
Disorder MIM:

608780

Disorder Html: Trichothiodystrophy, complementation group A, 601675 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_207118 NP_997001 general transcription factor IIH subunit 5



Homo sapiens (human) GTF2H5 NP_997001.1
Pan troglodytes (chimpanzee) GTF2H5 XP_001145020.1
Macaca mulatta (Rhesus monkey) GTF2H5 XP_001092693.1
Canis lupus familiaris (dog) GTF2H5 XP_005615600.1
Bos taurus (cattle) GTF2H5 NP_001035627.1
Mus musculus (house mouse) Gtf2h5 NP_852057.2
Rattus norvegicus (Norway rat) Gtf2h5 NP_001119560.1
Gallus gallus (chicken) GTF2H5 NP_001091004.1
Danio rerio (zebrafish) gtf2h5 NP_001127834.2
Drosophila melanogaster (fruit fly) Tfb5 NP_001137791.2
Xenopus (Silurana) tropicalis (western clawed frog) gtf2h5 NP_001017298.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GTF2H5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GTF2H5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu22965
NM_207118 Homo sapiens general transcription factor IIH, polypeptide 5 (GTF2H5), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22965
Clone ID Related Accession (Same CDS sequence) NM_207118
Accession Version NM_207118.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 216bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 05-JUN-2015
Organism Homo sapiens (human)
Product general transcription factor IIH subunit 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN403888.1, BC056906.1, AL590703.15, AL133347.28 and BQ004444.1. This sequence is a reference standard in the RefSeqGene project. On Mar 12, 2009 this sequence version replaced gi:46359854. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CN403888.1, AK055106.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
ATGGTCAACG TCTTGAAAGG AGTGCTTATA GAATGTGATC CTGCCATGAA GCAGTTTCTG 
CTGTACTTGG ATGAGTCCAA TGCCCTGGGG AAGAAGTTCA TCATTCAAGA CATTGATGAC
ACTCACGTCT TTGTAATAGC AGAATTGGTT AATGTCCTCC AGGAGCGAGT GGGTGAATTA
ATGGACCAAA ATGCTTTTTC CCTTACCCAG AAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_997001.1
CDS84..299
Misc Feature(1)75..77(+)
Misc Feature(2)87..293(+)
Misc Feature(3)288..290(+)
Misc Feature(4)288..290(+)
Exon (1)1..49
Gene:GTF2H5
Gene Synonym:
Exon (2)50..118
Gene:GTF2H5
Gene Synonym:
Exon (3)119..7486
Gene:GTF2H5
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_207118
Organism Homo sapiens (human)
Definition Homo sapiens general transcription factor IIH, polypeptide 5 (GTF2H5), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_207118

ORF Insert Sequence:

1
61
121
181
ATGGTCAACG TCTTGAAAGG AGTGCTTATA GAATGTGATC CTGCCATGAA GCAGTTTCTG 
CTGTACTTGG ATGAGTCCAA TGCCCTGGGG AAGAAGTTCA TCATTCAAGA CATTGATGAC
ACTCACGTCT TTGTAATAGC AGAATTGGTT AATGTCCTCC AGGAGCGAGT GGGTGAATTA
ATGGACCAAA ATGCTTTTTC CCTTACCCAG AAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

TFIIH subunit alterations causing xeroderma pigmentosum and trichothiodystrophy specifically disturb several steps during transcription
Am. J. Hum. Genet. 96 (2), 194-207 (2015)
Singh A, Compe E, Le May N and Egly JM.


book

Genome-wide association study of smoking behaviours among Bangladeshi adults
J. Med. Genet. 51 (5), 327-333 (2014)
Argos M, Tong L, Pierce BL, Rakibuz-Zaman M, Ahmed A, Islam T, Rahman M, Paul-Brutus R, Rahaman R, Roy S, Jasmine F, Kibriya MG and Ahsan H.


book

In vivo interactions of TTDA mutant proteins within TFIIH
J. Cell. Sci. 126 (PT 15), 3278-3283 (2013)
Nonnekens J, Cabantous S, Slingerland J, Mari PO and Giglia-Mari G.


book

Disruption of TTDA results in complete nucleotide excision repair deficiency and embryonic lethality
PLoS Genet. 9 (4), E1003431 (2013)
Theil AF, Nonnekens J, Steurer B, Mari PO, de Wit J, Lemaitre C, Marteijn JA, Raams A, Maas A, Vermeij M, Essers J, Hoeijmakers JH, Giglia-Mari G and Vermeulen W.


book

Variation within DNA repair pathway genes and risk of multiple sclerosis
Am. J. Epidemiol. 172 (2), 217-224 (2010)
Briggs FB, Goldstein BA, McCauley JL, Zuvich RL, De Jager PL, Rioux JD, Ivinson AJ, Compston A, Hafler DA, Hauser SL, Oksenberg JR, Sawcer SJ, Pericak-Vance MA, Haines JL and Barcellos LF.


book

Dynamic interaction of TTDA with TFIIH is stabilized by nucleotide excision repair in living cells
PLoS Biol. 4 (6), E156 (2006)
Giglia-Mari G, Miquel C, Theil AF, Mari PO, Hoogstraten D, Ng JM, Dinant C, Hoeijmakers JH and Vermeulen W.


book

p8/TTD-A as a repair-specific TFIIH subunit
Mol. Cell 21 (2), 215-226 (2006)
Coin F, Proietti De Santis L, Nardo T, Zlobinskaya O, Stefanini M and Egly JM.


book

A new, tenth subunit of TFIIH is responsible for the DNA repair syndrome trichothiodystrophy group A
Nat. Genet. 36 (7), 714-719 (2004)
Giglia-Mari G, Coin F, Ranish JA, Hoogstraten D, Theil A, Wijgers N, Jaspers NG, Raams A, Argentini M, van der Spek PJ, Botta E, Stefanini M, Egly JM, Aebersold R, Hoeijmakers JH and Vermeulen W.


book

The DNA sequence and analysis of human chromosome 6
Nature 425 (6960), 805-811 (2003)
Mungall AJ, Palmer SA, Sims SK, Edwards CA, Ashurst JL, Wilming L, Jones MC, Horton R, Hunt SE, Scott CE, Gilbert JG, Clamp ME, Bethel G, Milne S, Ainscough R, Almeida JP, Ambrose KD, Andrews TD, Ashwell RI, Babbage AK, Bagguley CL, Bailey J, Banerjee R, Barker DJ, Barlow KF, Bates K, Beare DM, Beasley H, Beasley O, Bird CP, Blakey S, Bray-Allen S, Brook J, Brown AJ, Brown JY, Burford DC, Burrill W, Burton J, Carder C, Carter NP, Chapman JC, Clark SY, Clark G, Clee CM, Clegg S, Cobley V, Collier RE, Collins JE, Colman LK, Corby NR, Coville GJ, Culley KM, Dhami P, Davies J, Dunn M, Earthrowl ME, Ellington AE, Evans KA, Faulkner L, Francis MD, Frankish A, Frankland J, French L, Garner P, Garnett J, Ghori MJ, Gilby LM, Gillson CJ, Glithero RJ, Grafham DV, Grant M, Gribble S, Griffiths C, Griffiths M, Hall R, Halls KS, Hammond S, Harley JL, Hart EA, Heath PD, Heathcott R, Holmes SJ, Howden PJ, Howe KL, Howell GR, Huckle E, Humphray SJ, Humphries MD, Hunt AR, Johnson CM, Joy AA, Kay M, Keenan SJ, Kimberley AM, King A, Laird GK, Langford C, Lawlor S, Leongamornlert DA, Leversha M, Lloyd CR, Lloyd DM, Loveland JE, Lovell J, Martin S, Mashreghi-Mohammadi M, Maslen GL, Matthews L, McCann OT, McLaren SJ, McLay K, McMurray A, Moore MJ, Mullikin JC, Niblett D, Nickerson T, Novik KL, Oliver K, Overton-Larty EK, Parker A, Patel R, Pearce AV, Peck AI, Phillimore B, Phillips S, Plumb RW, Porter KM, Ramsey Y, Ranby SA, Rice CM, Ross MT, Searle SM, Sehra HK, Sheridan E, Skuce CD, Smith S, Smith M, Spraggon L, Squares SL, Steward CA, Sycamore N, Tamlyn-Hall G, Tester J, Theaker AJ, Thomas DW, Thorpe A, Tracey A, Tromans A, Tubby B, Wall M, Wallis JM, West AP, White SS, Whitehead SL, Whittaker H, Wild A, Willey DJ, Wilmer TE, Wood JM, Wray PW, Wyatt JC, Young L, Younger RM, Bentley DR, Coulson A, Durbin R, Hubbard T, Sulston JE, Dunham I, Rogers J and Beck S.


book

Molecular evolution of the second ancient human mariner transposon, Hsmar2, illustrates patterns of neutral evolution in the human genome lineage
Gene 205 (1-2), 219-228 (1997)
Robertson HM and Martos R.


 
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