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MAD2L1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MAD2L1
Entrez Gene ID 4085
Full Name MAD2 mitotic arrest deficient-like 1 (yeast)
Synonyms HSMAD2, MAD2
General protein information
Preferred Names
mitotic spindle assembly checkpoint protein MAD2A
Names
mitotic spindle assembly checkpoint protein MAD2A
MAD2-like protein 1
mitotic arrest deficient 2-like protein 1
mitotic arrest deficient, yeast, homolog-like 1
MAD2 (mitotic arrest deficient, yeast, homolog)-like 1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

4

4q27

Summary MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

601467

Disorder Html:

mRNA and Protein(s)

mRNA Protein Name
NM_002358 NP_002349 mitotic spindle assembly checkpoint protein MAD2A

KEGG
hsa04110 Cell cycle
hsa04114 Oocyte meiosis
hsa04914 Progesterone-mediated oocyte maturation
hsa05166 HTLV-I infection
Reactome
R-HSA-162582 Signal Transduction
R-HSA-194315 Signaling by Rho GTPases
R-HSA-195258 RHO GTPase Effectors
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-1640170 Cell Cycle
R-HSA-69620 Cell Cycle Checkpoints
R-HSA-141424 Amplification of signal from the kinetochores
R-HSA-141405 Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components
R-HSA-69278 Cell Cycle, Mitotic
R-HSA-69618 Mitotic Spindle Checkpoint
R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-HSA-68886 M Phase
R-HSA-2555396 Mitotic Metaphase and Anaphase
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-HSA-68877 Mitotic Prometaphase
R-HSA-453276 Regulation of mitotic cell cycle
R-HSA-176814 Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins
R-HSA-174143 APC/C-mediated degradation of cell cycle proteins
R-HSA-68882 Mitotic Anaphase
R-HSA-179409 APC-Cdc20 mediated degradation of Nek2A
R-HSA-179419 APC:Cdc20 mediated degradation of cell cycle proteins prior to satisfation of the cell cycle checkpoint
R-HSA-176408 Regulation of APC/C activators between G1/S and early anaphase
R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
WikiPathways
WP179 Cell cycle


Homo sapiens (human) MAD2L1 NP_002349.1
Pan troglodytes (chimpanzee) MAD2L1 XP_003310508.1
Macaca mulatta (Rhesus monkey) MAD2L1 XP_001097054.2
Canis lupus familiaris (dog) LOC476070 XP_533278.2
Bos taurus (cattle) MAD2L1 NP_001178442.1
Mus musculus (house mouse) Mad2l1 NP_062372.2
Rattus norvegicus (Norway rat) Mad2l1 NP_001100064.1
Gallus gallus (chicken) MAD2L1 NP_001264678.1
Danio rerio (zebrafish) mad2l1 NP_001017739.1
Drosophila melanogaster (fruit fly) mad2 NP_647991.1
Caenorhabditis elegans mdf-2 NP_001023563.1
Arabidopsis thaliana (thale cress) MAD2 NP_189227.1
Xenopus (Silurana) tropicalis (western clawed frog) mad2l1 NP_001096528.1

Component

ID Name Evidence
GO:0000776 kinetochore IDA
GO:0000776 kinetochore IDA
GO:0000777 condensed chromosome kinetochore IEA
GO:0000922 spindle pole IEA
GO:0005634 nucleus IDA
GO:0005643 nuclear pore IDA
GO:0005694 chromosome IEA
GO:0005737 cytoplasm IEA
GO:0005829 cytosol EXP
GO:0005829 cytosol IDA
GO:0005829 cytosol TAS
GO:0048471 perinuclear region of cytoplasm IDA

Function

ID Name Evidence
GO:0005515 protein binding IPI
GO:0042803 protein homodimerization activity IPI

Process

ID Name Evidence
GO:0000070 mitotic sister chromatid segregation IEA
GO:0000075 cell cycle checkpoint TAS
GO:0000087 M phase of mitotic cell cycle TAS
GO:0000236 mitotic prometaphase TAS
GO:0000278 mitotic cell cycle TAS
GO:0007093 mitotic cell cycle checkpoint IDA
GO:0007094 mitotic cell cycle spindle assembly checkpoint TAS
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process EXP
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process TAS
GO:0042177 negative regulation of protein catabolic process IDA
GO:0043066 negative regulation of apoptosis IMP
GO:0051301 cell division IEA
GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle EXP
GO:0051439 regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle TAS
GO:0060564 negative regulation of mitotic anaphase-promoting complex activity IDA
GO:0090267 positive regulation of mitotic cell cycle spindle assembly checkpoint IMP

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MAD2L1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAD2L1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu24019
NM_002358 Homo sapiens MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.30
$99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu24019
Accession Version NM_002358.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 618bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mitotic spindle assembly checkpoint protein MAD2A
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AA443733.1, AK313827.1, AK223433.1 and BC000356.2. On Jul 30, 2008 this sequence version replaced gi:6466452. Summary: MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000356.2, BC005945.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGGCGCTGC AGCTCTCCCG GGAGCAGGGA ATCACCCTGC GCGGGAGCGC CGAAATCGTG 
GCCGAGTTCT TCTCATTCGG CATCAACAGC ATTTTATATC AGCGTGGCAT ATATCCATCT
GAAACCTTTA CTCGAGTGCA GAAATACGGA CTCACCTTGC TTGTAACTAC TGATCTTGAG
CTCATAAAAT ACCTAAATAA TGTGGTGGAA CAACTGAAAG ATTGGTTATA CAAGTGTTCA
GTTCAGAAAC TGGTTGTAGT TATCTCAAAT ATTGAAAGTG GTGAGGTCCT GGAAAGATGG
CAGTTTGATA TTGAGTGTGA CAAGACTGCA AAAGATGACA GTGCACCCAG AGAAAAGTCT
CAGAAAGCTA TCCAGGATGA AATCCGTTCA GTGATCAGAC AGATCACAGC TACGGTGACA
TTTCTGCCAC TGTTGGAAGT TTCTTGTTCA TTTGATCTGC TGATTTATAC AGACAAAGAT
TTGGTTGTAC CTGAAAAATG GGAAGAGTCG GGACCACAGT TTATTACCAA TTCTGAGGAA
GTCCGCCTTC GTTCATTTAC TACTACAATC CACAAAGTAA ATAGCATGGT GGCCTACAAA
ATTCCTGTCA ATGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_002349.1
CDS125..742
Misc Feature(1)128..130(+)
Misc Feature(2)158..697(+)
Misc Feature(3)632..634(+)
Misc Feature(4)632..634(+)
Misc Feature(5)656..658(+)
Misc Feature(6)656..658(+)
Misc Feature(7)707..739(+)
Misc Feature(8)707..709(+)
Misc Feature(9)707..709(+)
Exon (1)1..197
Gene:MAD2L1
Gene Synonym:
Exon (2)198..344
Gene:MAD2L1
Gene Synonym:
Exon (3)345..465
Gene:MAD2L1
Gene Synonym:
Exon (4)466..569
Gene:MAD2L1
Gene Synonym:
Exon (5)570..1438
Gene:MAD2L1
Gene Synonym:
Translation
Position Chain Variation Link
13 13 a, g dbSNP:541212332
16 16 c, g dbSNP:572197728
19 19 g, t dbSNP:142741206
34 34 a, t dbSNP:777122300
45 45 c, t dbSNP:186459665
65 65 c, g dbSNP:576351571
67 67 a, g dbSNP:538637470
80 80 -, gc dbSNP:538690213
85 85 a, c dbSNP:375432097
86 86 a, g dbSNP:763221187
95 95 a, g dbSNP:750202365
98 98 a, c, g dbSNP:761723829
104 104 c, g, t dbSNP:768206152
107 107 g, t dbSNP:536937745
113 113 g, t dbSNP:372801671
114 114 a, t dbSNP:771733311
121 121 a, g dbSNP:745467143
125 125 a, g dbSNP:778000548
129 129 c, t dbSNP:770186875
138 138 a, t dbSNP:748657577
139 139 c, g dbSNP:781707248
141 141 c, t dbSNP:755503995
143 143 c, g dbSNP:375700698
144 144 a, g dbSNP:144346584
145 145 a, g dbSNP:758702337
146 146 a, g dbSNP:750653937
155 155 a, g dbSNP:765001112
163 163 g, t dbSNP:757054374
164 164 c, t dbSNP:753762145
169 169 a, g dbSNP:764048695
190 190 a, g dbSNP:140487496
197 197 c, t dbSNP:773884131
203 203 a, g dbSNP:767071365
205 205 c, t dbSNP:759063752
220 220 a, g dbSNP:758541913
223 223 a, t dbSNP:766080399
228 228 a, g dbSNP:762457318
232 232 c, g dbSNP:777140652
235 235 a, g dbSNP:769138377
243 243 c, g dbSNP:747580882
244 244 a, t dbSNP:553500398
250 250 c, g dbSNP:368678145
255 255 c, t dbSNP:745994403
262 262 a, g dbSNP:778806579
271 271 c, t dbSNP:199627005
315 315 a, t dbSNP:749442266
317 317 a, c, t dbSNP:2193126
328 328 a, g dbSNP:755890062
335 335 c, t dbSNP:752678732
337 337 a, g dbSNP:781075650
338 338 a, c dbSNP:2859834
349 349 g, t dbSNP:769616080
357 357 a, g dbSNP:747966227
358 358 c, g dbSNP:149949223
359 359 a, t dbSNP:368386354
364 364 a, t dbSNP:577220356
369 369 a, c, g dbSNP:557538317
379 379 a, t dbSNP:139205919
388 388 c, t dbSNP:757962988
392 392 a, g dbSNP:750143980
395 395 a, g dbSNP:765016504
407 407 a, g dbSNP:761511460
410 410 a, g dbSNP:753122638
420 420 g, t dbSNP:767704519
469 469 a, g dbSNP:778506790
471 471 c, t dbSNP:756971877
480 480 a, t dbSNP:372366391
483 483 c, t dbSNP:150812263
486 486 a, c dbSNP:370388308
487 487 a, g dbSNP:752146697
488 488 a, t dbSNP:766825408
491 491 a, g dbSNP:763399814
509 509 c, t dbSNP:773286573
522 522 a, g dbSNP:78047690
538 538 a, g dbSNP:529786403
553 553 a, g dbSNP:61752609
570 570 a, g dbSNP:371009570
578 578 c, g dbSNP:776735919
580 580 a, t dbSNP:764247276
586 586 a, g dbSNP:376755613
602 602 c, g dbSNP:775149389
611 611 g, t dbSNP:138835443
613 613 a, g dbSNP:745854645
624 624 c, g dbSNP:774405611
631 631 a, g dbSNP:370042213
634 634 a, g dbSNP:748779990
635 635 a, g dbSNP:146087467
649 649 a, t dbSNP:769344064
667 667 a, c dbSNP:747834213
668 668 c, t dbSNP:780456520
669 669 a, g dbSNP:758740535
674 674 c, t dbSNP:750970089
675 675 a, g dbSNP:779431364
679 679 a, g dbSNP:757737153
689 689 a, g dbSNP:200592551
692 692 a, g dbSNP:61752608
700 700 a, g dbSNP:760870683
706 706 c, t dbSNP:752951123
710 710 a, g dbSNP:767464526
711 711 a, t dbSNP:201933811
722 722 a, g dbSNP:774246429
727 727 g, t dbSNP:770795554
735 735 a, g dbSNP:762888359
738 738 a, c dbSNP:772601160
751 751 g, t dbSNP:769335442
755 755 a, g dbSNP:747806570
759 759 a, g dbSNP:373089732
768 768 c, t dbSNP:187158111
781 781 a, g dbSNP:746259085
784 784 c, t dbSNP:779192940
785 785 a, g dbSNP:375995529
790 790 c, t dbSNP:754362286
801 801 a, g dbSNP:2908990
813 813 c, t dbSNP:569591328
824 824 g, t dbSNP:764147614
870 870 a, g dbSNP:556120384
877 877 g, t dbSNP:535968010
878 878 g, t dbSNP:567328380
887 887 c, t dbSNP:547628264
888 888 g, t dbSNP:182761177
897 897 -, t dbSNP:369801593
916 916 c, g dbSNP:756809162
924 924 a, g dbSNP:571442973
943 943 c, t dbSNP:551418862
951 951 a, g dbSNP:531634440
981 981 c, t dbSNP:190679989
1040 1040 a, g dbSNP:777973088
1053 1053 a, t dbSNP:530141070
1089 1089 c, t dbSNP:375160196
1098 1098 a, g dbSNP:763729075
1118 1118 c, g dbSNP:762890062
1161 1161 g, t dbSNP:543761963
1169 1169 c, t dbSNP:765364467
1216 1216 a, t dbSNP:759578406
1298 1298 c, t dbSNP:530151759
1347 1347 g, t dbSNP:567503861
1364 1364 -, ca dbSNP:199889773
1373 1373 g, t dbSNP:776769703
1381 1381 c, g dbSNP:771210860
1384 1384 c, g dbSNP:187940971

Target ORF information:

RefSeq Version NM_002358
Organism Homo sapiens (human)
Definition Homo sapiens MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002358

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCGCTGC AGCTCTCCCG GGAGCAGGGA ATCACCCTGC GCGGGAGCGC CGAAATCGTG 
GCCGAGTTCT TCTCATTCGG CATCAACAGC ATTTTATATC AGCGTGGCAT ATATCCATCT
GAAACCTTTA CTCGAGTGCA GAAATACGGA CTCACCTTGC TTGTAACTAC TGATCTTGAG
CTCATAAAAT ACCTAAATAA TGTGGTGGAA CAACTGAAAG ATTGGTTATA CAAGTGTTCA
GTTCAGAAAC TGGTTGTAGT TATCTCAAAT ATTGAAAGTG GTGAGGTCCT GGAAAGATGG
CAGTTTGATA TTGAGTGTGA CAAGACTGCA AAAGATGACA GTGCACCCAG AGAAAAGTCT
CAGAAAGCTA TCCAGGATGA AATCCGTTCA GTGATCAGAC AGATCACAGC TACGGTGACA
TTTCTGCCAC TGTTGGAAGT TTCTTGTTCA TTTGATCTGC TGATTTATAC AGACAAAGAT
TTGGTTGTAC CTGAAAAATG GGAAGAGTCG GGACCACAGT TTATTACCAA TTCTGAGGAA
GTCCGCCTTC GTTCATTTAC TACTACAATC CACAAAGTAA ATAGCATGGT GGCCTACAAA
ATTCCTGTCA ATGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Synthetic genetic array screen identifies PP2A as a therapeutic target in Mad2-overexpressing tumors
Proc. Natl. Acad. Sci. U.S.A. 111 (4), 1628-1633 (2014)
Bian Y, Kitagawa R, Bansal PK, Fujii Y, Stepanov A and Kitagawa K.


book

Expression of budding uninhibited by benzimidazoles-1 and mitotic arrest deficient-2 in endometrial carcinoma and its significance
Eur. J. Gynaecol. Oncol. 35 (1), 44-47 (2014)
Zhao,Q., Bian,A.P., Zhang,Y., Qin,L., Shi,H.R. and Su,K.


book

Spindle assembly checkpoint robustness requires Tpr-mediated regulation of Mad1/Mad2 proteostasis
J. Cell Biol. 203 (6), 883-893 (2013)
Schweizer,N., Ferras,C., Kern,D.M., Logarinho,E., Cheeseman,I.M. and Maiato,H.


book

Monopolar spindle 1 (MPS1) kinase promotes production of closed MAD2 (C-MAD2) conformer and assembly of the mitotic checkpoint complex
J. Biol. Chem. 288 (49), 35149-35158 (2013)
Tipton AR, Ji W, Sturt-Gillespie B, Bekier ME 2nd, Wang K, Taylor WR and Liu ST.


book

High expression of spindle assembly checkpoint proteins CDC20 and MAD2 is associated with poor prognosis in urothelial bladder cancer
Virchows Arch. 463 (5), 681-687 (2013)
Choi JW, Kim Y, Lee JH and Kim YS.


book

Map location and gene structure of the Homo sapiens mitotic arrest deficient 2 (MAD2L1) gene at 4q27
Genomics 49 (3), 475-478 (1998)
Krishnan R, Goodman B, Jin DY, Jeang KT, Collins C, Stetten G and Spencer F.


book

Human T cell leukemia virus type 1 oncoprotein Tax targets the human mitotic checkpoint protein MAD1
Cell 93 (1), 81-91 (1998)
Jin DY, Spencer F and Jeang KT.


book

Interaction of MAD2 with the carboxyl terminus of the insulin receptor but not with the IGFIR. Evidence for release from the insulin receptor after activation
J. Biol. Chem. 272 (15), 10035-10040 (1997)
O'Neill TJ, Zhu Y and Gustafson TA.


book

Assignment of mitotic arrest deficient protein 2 (MAD2L1) to human chromosome band 5q23.3 by in situ hybridization
Cytogenet. Cell Genet. 78 (1), 63-64 (1997)
Xu L, Deng HX, Yang Y, Xia JH, Hung WY and Siddque T.


book

Identification of a human mitotic checkpoint gene: hsMAD2
Science 274 (5285), 246-248 (1996)
Li Y and Benezra R.


 
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