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MAD2L1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MAD2L1
Entrez Gene ID 4085
Full Name MAD2 mitotic arrest deficient-like 1 (yeast)
Synonyms HSMAD2, MAD2
General protein information
Preferred Names
mitotic spindle assembly checkpoint protein MAD2A
Names
mitotic spindle assembly checkpoint protein MAD2A
MAD2-like protein 1
mitotic arrest deficient 2-like protein 1
mitotic arrest deficient, yeast, homolog-like 1
MAD2 (mitotic arrest deficient, yeast, homolog)-like 1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

4

4q27

Summary MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

601467


mRNA and Protein(s)

mRNA Protein Name
NM_002358 NP_002349 mitotic spindle assembly checkpoint protein MAD2A



Homo sapiens (human) MAD2L1 NP_002349.1
Pan troglodytes (chimpanzee) MAD2L1 XP_003310508.1
Macaca mulatta (Rhesus monkey) MAD2L1 XP_001097054.2
Canis lupus familiaris (dog) LOC476070 XP_533278.2
Bos taurus (cattle) MAD2L1 NP_001178442.1
Mus musculus (house mouse) Mad2l1 NP_062372.2
Rattus norvegicus (Norway rat) Mad2l1 NP_001100064.1
Gallus gallus (chicken) MAD2L1 NP_001264678.1
Danio rerio (zebrafish) mad2l1 NP_001017739.1
Drosophila melanogaster (fruit fly) mad2 NP_647991.1
Caenorhabditis elegans mdf-2 NP_001023563.1
Arabidopsis thaliana (thale cress) MAD2 NP_189227.1
Xenopus (Silurana) tropicalis (western clawed frog) mad2l1 NP_001096528.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MAD2L1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAD2L1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu24019
NM_002358 Homo sapiens MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu24019
Clone ID Related Accession (Same CDS sequence) NM_002358
Accession Version NM_002358.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 618bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mitotic spindle assembly checkpoint protein MAD2A
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AA443733.1, AK313827.1, AK223433.1 and BC000356.2. On Jul 30, 2008 this sequence version replaced gi:6466452. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000356.2, BC005945.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGGCGCTGC AGCTCTCCCG GGAGCAGGGA ATCACCCTGC GCGGGAGCGC CGAAATCGTG 
GCCGAGTTCT TCTCATTCGG CATCAACAGC ATTTTATATC AGCGTGGCAT ATATCCATCT
GAAACCTTTA CTCGAGTGCA GAAATACGGA CTCACCTTGC TTGTAACTAC TGATCTTGAG
CTCATAAAAT ACCTAAATAA TGTGGTGGAA CAACTGAAAG ATTGGTTATA CAAGTGTTCA
GTTCAGAAAC TGGTTGTAGT TATCTCAAAT ATTGAAAGTG GTGAGGTCCT GGAAAGATGG
CAGTTTGATA TTGAGTGTGA CAAGACTGCA AAAGATGACA GTGCACCCAG AGAAAAGTCT
CAGAAAGCTA TCCAGGATGA AATCCGTTCA GTGATCAGAC AGATCACAGC TACGGTGACA
TTTCTGCCAC TGTTGGAAGT TTCTTGTTCA TTTGATCTGC TGATTTATAC AGACAAAGAT
TTGGTTGTAC CTGAAAAATG GGAAGAGTCG GGACCACAGT TTATTACCAA TTCTGAGGAA
GTCCGCCTTC GTTCATTTAC TACTACAATC CACAAAGTAA ATAGCATGGT GGCCTACAAA
ATTCCTGTCA ATGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002349.1
CDS125..742
Misc Feature(1)128..130(+)
Misc Feature(2)158..697(+)
Misc Feature(3)632..634(+)
Misc Feature(4)632..634(+)
Misc Feature(5)656..658(+)
Misc Feature(6)656..658(+)
Misc Feature(7)707..739(+)
Misc Feature(8)707..709(+)
Misc Feature(9)707..709(+)
Exon (1)1..197
Gene:MAD2L1
Gene Synonym:
Exon (2)198..344
Gene:MAD2L1
Gene Synonym:
Exon (3)345..465
Gene:MAD2L1
Gene Synonym:
Exon (4)466..569
Gene:MAD2L1
Gene Synonym:
Exon (5)570..1438
Gene:MAD2L1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002358
Organism Homo sapiens (human)
Definition Homo sapiens MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002358

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCGCTGC AGCTCTCCCG GGAGCAGGGA ATCACCCTGC GCGGGAGCGC CGAAATCGTG 
GCCGAGTTCT TCTCATTCGG CATCAACAGC ATTTTATATC AGCGTGGCAT ATATCCATCT
GAAACCTTTA CTCGAGTGCA GAAATACGGA CTCACCTTGC TTGTAACTAC TGATCTTGAG
CTCATAAAAT ACCTAAATAA TGTGGTGGAA CAACTGAAAG ATTGGTTATA CAAGTGTTCA
GTTCAGAAAC TGGTTGTAGT TATCTCAAAT ATTGAAAGTG GTGAGGTCCT GGAAAGATGG
CAGTTTGATA TTGAGTGTGA CAAGACTGCA AAAGATGACA GTGCACCCAG AGAAAAGTCT
CAGAAAGCTA TCCAGGATGA AATCCGTTCA GTGATCAGAC AGATCACAGC TACGGTGACA
TTTCTGCCAC TGTTGGAAGT TTCTTGTTCA TTTGATCTGC TGATTTATAC AGACAAAGAT
TTGGTTGTAC CTGAAAAATG GGAAGAGTCG GGACCACAGT TTATTACCAA TTCTGAGGAA
GTCCGCCTTC GTTCATTTAC TACTACAATC CACAAAGTAA ATAGCATGGT GGCCTACAAA
ATTCCTGTCA ATGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Synthetic genetic array screen identifies PP2A as a therapeutic target in Mad2-overexpressing tumors
Proc. Natl. Acad. Sci. U.S.A. 111 (4), 1628-1633 (2014)
Bian Y, Kitagawa R, Bansal PK, Fujii Y, Stepanov A and Kitagawa K.


book

Expression of budding uninhibited by benzimidazoles-1 and mitotic arrest deficient-2 in endometrial carcinoma and its significance
Eur. J. Gynaecol. Oncol. 35 (1), 44-47 (2014)
Zhao,Q., Bian,A.P., Zhang,Y., Qin,L., Shi,H.R. and Su,K.


book

Spindle assembly checkpoint robustness requires Tpr-mediated regulation of Mad1/Mad2 proteostasis
J. Cell Biol. 203 (6), 883-893 (2013)
Schweizer,N., Ferras,C., Kern,D.M., Logarinho,E., Cheeseman,I.M. and Maiato,H.


book

Monopolar spindle 1 (MPS1) kinase promotes production of closed MAD2 (C-MAD2) conformer and assembly of the mitotic checkpoint complex
J. Biol. Chem. 288 (49), 35149-35158 (2013)
Tipton AR, Ji W, Sturt-Gillespie B, Bekier ME 2nd, Wang K, Taylor WR and Liu ST.


book

High expression of spindle assembly checkpoint proteins CDC20 and MAD2 is associated with poor prognosis in urothelial bladder cancer
Virchows Arch. 463 (5), 681-687 (2013)
Choi JW, Kim Y, Lee JH and Kim YS.


book

Map location and gene structure of the Homo sapiens mitotic arrest deficient 2 (MAD2L1) gene at 4q27
Genomics 49 (3), 475-478 (1998)
Krishnan R, Goodman B, Jin DY, Jeang KT, Collins C, Stetten G and Spencer F.


book

Human T cell leukemia virus type 1 oncoprotein Tax targets the human mitotic checkpoint protein MAD1
Cell 93 (1), 81-91 (1998)
Jin DY, Spencer F and Jeang KT.


book

Interaction of MAD2 with the carboxyl terminus of the insulin receptor but not with the IGFIR. Evidence for release from the insulin receptor after activation
J. Biol. Chem. 272 (15), 10035-10040 (1997)
O'Neill TJ, Zhu Y and Gustafson TA.


book

Assignment of mitotic arrest deficient protein 2 (MAD2L1) to human chromosome band 5q23.3 by in situ hybridization
Cytogenet. Cell Genet. 78 (1), 63-64 (1997)
Xu L, Deng HX, Yang Y, Xia JH, Hung WY and Siddque T.


book

Identification of a human mitotic checkpoint gene: hsMAD2
Science 274 (5285), 246-248 (1996)
Li Y and Benezra R.


 
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