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MAGEA2 melanoma antigen family A2 [Homo sapiens (human)]

Gene Symbol MAGEA2
Entrez Gene ID 4101
Full Name melanoma antigen family A2
Synonyms CT1.2, MAGE2, MAGEA2A
General protein information
Preferred Names
melanoma-associated antigen 2
Names
melanoma-associated antigen 2
MAGE-2 antigen
melanoma antigen 2
cancer/testis antigen 1.2
cancer/testis antigen family 1, member 2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xq28

Summary This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci. Alternatively spliced transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

300173

Disorder Html:





The following MAGEA2 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGEA2 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu00411 NM_001282504 Homo sapiens melanoma antigen family A2 (MAGEA2), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$199
OHu00411 NM_175742 Homo sapiens melanoma antigen family A2 (MAGEA2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$199
OHu00411 NM_001282502 Homo sapiens melanoma antigen family A2 (MAGEA2), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$199
OHu00411 NM_175743 Homo sapiens melanoma antigen family A2 (MAGEA2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$199
OHu00411 NM_001282501 Homo sapiens melanoma antigen family A2 (MAGEA2), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$199
OHu00411 NM_001282505 Homo sapiens melanoma antigen family A2 (MAGEA2), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$199
OHu00411 NM_005361 Homo sapiens melanoma antigen family A2 (MAGEA2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$199
OHu00411 XM_011531159 PREDICTED: Homo sapiens melanoma antigen family A2 (MAGEA2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$199

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu00411
Accession Version NM_001282504.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu00411D_COA.pdf (pdf)
Update Date 08-FEB-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290443.1, DR158683.1, BC108720.1 and CA429655.1. On or before Sep 12, 2013 this sequence version replaced gi:530422782, gi:530430503. Summary: This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci. Alternatively spliced transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (6) lacks three internal exons in the 5' UTR region, compared to variant 3. Variants 1 through 7 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BF791379.1, DR158683.1 [ECO:0000332] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001269433.1
CDS229..1173
Misc Feature(1)52..54(+)
Misc Feature(2)235..513(+)
Misc Feature(3)574..1086(+)
Exon (1)1..97
Gene:MAGEA2
Gene Synonym:
Exon (2)98..163
Gene:MAGEA2
Gene Synonym:
Exon (3)164..1729
Gene:MAGEA2
Gene Synonym:
Translation
Position Chain Variation Link
968 968 c, t dbSNP:202231943
969 969 c, g dbSNP:200459473
976 976 c, t dbSNP:201146248
1723 1723 c, t dbSNP:6627647

Target ORF information:

RefSeq Version NM_001282504
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family A2 (MAGEA2), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282504

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00411
Accession Version NM_175742.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu00411D_COA.pdf (pdf)
Update Date 08-FEB-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290443.1, BM781931.1, BC108720.1 and CA429655.1. On Sep 12, 2013 this sequence version replaced gi:29029617. Summary: This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci. Alternatively spliced transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (2) has an additional exon in the 5' region and lacks an alternate exon in a downstream region, compared to variant 3. Variants 1 through 7 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM781931.1, BM846411.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_786884.1
CDS510..1454
Misc Feature(1)375..377(+)
Misc Feature(2)516..794(+)
Misc Feature(3)855..1367(+)
Exon (1)1..97
Gene:MAGEA2
Gene Synonym:
Exon (2)98..179
Gene:MAGEA2
Gene Synonym:
Exon (3)180..258
Gene:MAGEA2
Gene Synonym:
Exon (4)259..378
Gene:MAGEA2
Gene Synonym:
Exon (5)379..444
Gene:MAGEA2
Gene Synonym:
Exon (6)445..2010
Gene:MAGEA2
Gene Synonym:
Translation
Position Chain Variation Link
1249 1249 c, t dbSNP:202231943
1250 1250 c, g dbSNP:200459473
1257 1257 c, t dbSNP:201146248
2004 2004 c, t dbSNP:6627647

Target ORF information:

RefSeq Version NM_175742
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family A2 (MAGEA2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_175742

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00411
Accession Version NM_001282502.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu00411D_COA.pdf (pdf)
Update Date 08-FEB-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290443.1, BC108720.1 and CA429655.1. On or before Sep 12, 2013 this sequence version replaced gi:530422780, gi:530430501. Summary: This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci. Alternatively spliced transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (5) lacks two internal exons in the 5' UTR region, compared to variant 3. Variants 1 through 7 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC108720.1, DR157723.1 [ECO:0000332] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001269431.1
CDS349..1293
Misc Feature(1)214..216(+)
Misc Feature(2)355..633(+)
Misc Feature(3)694..1206(+)
Exon (1)1..97
Gene:MAGEA2
Gene Synonym:
Exon (2)98..217
Gene:MAGEA2
Gene Synonym:
Exon (3)218..283
Gene:MAGEA2
Gene Synonym:
Exon (4)284..1849
Gene:MAGEA2
Gene Synonym:
Translation
Position Chain Variation Link
1088 1088 c, t dbSNP:202231943
1089 1089 c, g dbSNP:200459473
1096 1096 c, t dbSNP:201146248
1843 1843 c, t dbSNP:6627647

Target ORF information:

RefSeq Version NM_001282502
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family A2 (MAGEA2), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282502

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00411
Accession Version NM_175743.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu00411D_COA.pdf (pdf)
Update Date 08-FEB-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290443.1, BX282627.1, BC108720.1 and CA429655.1. On Sep 12, 2013 this sequence version replaced gi:29029619. Summary: This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci. Alternatively spliced transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (3) is the longest transcript. Variants 1 through 7 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG575302.1, BI859928.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_786885.1
CDS526..1470
Misc Feature(1)337..339(+)
Misc Feature(2)532..810(+)
Misc Feature(3)871..1383(+)
Exon (1)1..97
Gene:MAGEA2
Gene Synonym:
Exon (2)98..176
Gene:MAGEA2
Gene Synonym:
Exon (3)177..296
Gene:MAGEA2
Gene Synonym:
Exon (4)297..394
Gene:MAGEA2
Gene Synonym:
Exon (5)395..460
Gene:MAGEA2
Gene Synonym:
Exon (6)461..2026
Gene:MAGEA2
Gene Synonym:
Translation
Position Chain Variation Link
388 388 a, g dbSNP:6627646
1265 1265 c, t dbSNP:202231943
1266 1266 c, g dbSNP:200459473
1273 1273 c, t dbSNP:201146248
2020 2020 c, t dbSNP:6627647

Target ORF information:

RefSeq Version NM_175743
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family A2 (MAGEA2), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_175743

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00411
Accession Version NM_001282501.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu00411D_COA.pdf (pdf)
Update Date 08-FEB-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290443.1, BC063681.1 and CA429655.1. On or before Sep 12, 2013 this sequence version replaced gi:530422778, gi:530430499. Summary: This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci. Alternatively spliced transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (4) lacks an internal exon in the 5' UTR region, compared to variant 3. Variants 1 through 7 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK290443.1, CA489674.1 [ECO:0000332] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001269430.1
CDS447..1391
Misc Feature(1)258..260(+)
Misc Feature(2)453..731(+)
Misc Feature(3)792..1304(+)
Exon (1)1..97
Gene:MAGEA2
Gene Synonym:
Exon (2)98..217
Gene:MAGEA2
Gene Synonym:
Exon (3)218..315
Gene:MAGEA2
Gene Synonym:
Exon (4)316..381
Gene:MAGEA2
Gene Synonym:
Exon (5)382..1947
Gene:MAGEA2
Gene Synonym:
Translation
Position Chain Variation Link
309 309 a, g dbSNP:6627646
1186 1186 c, t dbSNP:202231943
1187 1187 c, g dbSNP:200459473
1194 1194 c, t dbSNP:201146248
1941 1941 c, t dbSNP:6627647

Target ORF information:

RefSeq Version NM_001282501
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family A2 (MAGEA2), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282501

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00411
Accession Version NM_001282505.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu00411D_COA.pdf (pdf)
Update Date 08-FEB-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290443.1, AU100045.1, BC108720.1 and CA429655.1. On or before Sep 12, 2013 this sequence version replaced gi:530422784, gi:530430505. Summary: This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci. Alternatively spliced transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (7) lacks four internal exons in the 5' UTR region, compared to variant 3. Variants 1 through 7 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC109188.1, AU100045.1 [ECO:0000332] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001269434.1
CDS163..1107
Misc Feature(1)52..54(+)
Misc Feature(2)169..447(+)
Misc Feature(3)508..1020(+)
Exon (1)1..97
Gene:MAGEA2
Gene Synonym:
Exon (2)98..1663
Gene:MAGEA2
Gene Synonym:
Translation
Position Chain Variation Link
902 902 c, t dbSNP:202231943
903 903 c, g dbSNP:200459473
910 910 c, t dbSNP:201146248
1657 1657 c, t dbSNP:6627647

Target ORF information:

RefSeq Version NM_001282505
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family A2 (MAGEA2), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282505

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00411
Accession Version NM_005361.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu00411D_COA.pdf (pdf)
Update Date 08-FEB-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC244102.3. On Sep 12, 2013 this sequence version replaced gi:29029616. Summary: This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci. Alternatively spliced transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 3. Variants 1 through 7 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: CA453951.1, BF793468.1 [ECO:0000331] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_005352.1
CDS524..1468
Misc Feature(1)389..391(+)
Misc Feature(2)530..808(+)
Misc Feature(3)869..1381(+)
Exon (1)1..272
Gene:MAGEA2
Gene Synonym:
Exon (2)273..392
Gene:MAGEA2
Gene Synonym:
Exon (3)393..458
Gene:MAGEA2
Gene Synonym:
Exon (4)459..2024
Gene:MAGEA2
Gene Synonym:
Translation
Position Chain Variation Link
1263 1263 c, t dbSNP:202231943
1264 1264 c, g dbSNP:200459473
1271 1271 c, t dbSNP:201146248
2018 2018 c, t dbSNP:6627647

Target ORF information:

RefSeq Version NM_005361
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family A2 (MAGEA2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005361

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00411
Accession Version XM_011531159.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu00411D_COA.pdf (pdf)
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011681.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011529461.1
CDS692..1636
Misc Feature(1)698..976(+)
Misc Feature(2)1037..1549(+)
Translation
Position Chain Variation Link
554 554 a, g dbSNP:6627646
1431 1431 c, t dbSNP:202231943
1432 1432 c, g dbSNP:200459473
1439 1439 c, t dbSNP:201146248
2186 2186 c, t dbSNP:6627647

Target ORF information:

RefSeq Version XM_011531159
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens melanoma antigen family A2 (MAGEA2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531159

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.