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MAGEA4 melanoma antigen family A4 [Homo sapiens (human)]

Gene Symbol MAGEA4
Entrez Gene ID 4103
Full Name melanoma antigen family A4
Synonyms CT1.4, MAGE-41, MAGE-X2, MAGE4, MAGE4A, MAGE4B
General protein information
Preferred Names
melanoma-associated antigen 4
Names
melanoma-associated antigen 4
MAGE-4 antigen
MAGE-41 antigen
MAGE-X2 antigen
cancer/testis antigen 1.4
cancer/testis antigen family 1, member 4
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xq28

Summary This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

300175

Disorder Html:
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu54401 XM_005274677 PREDICTED: Homo sapiens melanoma antigen family A4 (MAGEA4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu02154 XM_005274679 PREDICTED: Homo sapiens melanoma antigen family A4 (MAGEA4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu02154 XM_005274678 PREDICTED: Homo sapiens melanoma antigen family A4 (MAGEA4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu02154 NM_002362 Homo sapiens melanoma antigen family A4 (MAGEA4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu02154 NM_001011548 Homo sapiens melanoma antigen family A4 (MAGEA4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu02154 NM_001011549 Homo sapiens melanoma antigen family A4 (MAGEA4), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu02154 NM_001011550 Homo sapiens melanoma antigen family A4 (MAGEA4), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu54401D
Sequence Information ORF Nucleotide Sequence (Length: 1194bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011681.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530422788. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCCACTGA CTTGCGCATT GGGGGTTAGA GAGAAGCGAG CTGCTCTGAC CAGCCGCTTG 
GGATTGGCGG AGGGAAGCGG GCCAGGCCCT GTGAGGAGTC AAGGTTCTGA GCAGACAGGC
CAACCGGAGG ACAGGATTCC CTGGAGGCCA CAGAGGAGCA CCAAGGAGAA GATCTGCCTG
TGGGTCCCCA TTGCCCAGCT TTTGCCTGCA CTCTTGCCTG CTGCCCTGAC CAGAGTCATC
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005274734.1
CDS22..1215
Misc Feature(1)268..558(+)
Misc Feature(2)610..1122(+)
Translation
Position Chain Variation Link
128 128 a, c, t dbSNP:745816060
135 135 a, g dbSNP:774445671
140 140 a, g dbSNP:748368449
144 144 a, g dbSNP:368738694
145 145 c, t dbSNP:773613926
146 146 c, t dbSNP:763594807
147 147 a, g dbSNP:766855228
162 162 c, g dbSNP:777222146
171 171 a, g dbSNP:148460606
172 172 c, t dbSNP:764802119
186 186 g, t dbSNP:752191271
188 188 -, aga dbSNP:745310597
195 195 c, t dbSNP:758051896
198 198 c, t dbSNP:775056875
206 206 -, c dbSNP:769356179
207 207 c, t dbSNP:762298297
210 210 c, t dbSNP:183961725
211 211 a, g dbSNP:144129975
212 212 a, t dbSNP:761483501
219 219 a, g dbSNP:372125339
232 232 c, g dbSNP:767267374
233 233 c, t dbSNP:750267906
239 239 c, t dbSNP:756031183
244 244 a, g dbSNP:779923857
251 251 c, g dbSNP:1047246
259 259 a, g dbSNP:201648066
266 266 c, t dbSNP:777932203
272 272 a, g dbSNP:747163064
277 277 a, g dbSNP:771323726
278 278 a, g dbSNP:781676215
285 285 a, g dbSNP:746298946
289 289 c, t dbSNP:770283475
290 290 a, g dbSNP:775799958
291 291 c, t dbSNP:763535308
306 306 c, t dbSNP:200225562
307 307 a, g dbSNP:142773415
308 308 c, t dbSNP:780727589
316 316 c, g dbSNP:147367105
325 325 g, t dbSNP:767214466
326 326 c, t dbSNP:750215141
331 331 a, g dbSNP:760396181
335 335 c, t dbSNP:766211889
341 341 a, g, t dbSNP:753670573
342 342 c, t dbSNP:777687166
344 344 c, t dbSNP:772345662
345 345 a, g dbSNP:1047248
348 348 a, g dbSNP:150592561
350 350 c, t dbSNP:746953197
352 352 c, t dbSNP:61743686
358 358 a, t dbSNP:149323734
361 361 a, g dbSNP:749725686
374 374 c, t dbSNP:759301904
375 375 c, t dbSNP:369838724
380 380 a, t dbSNP:773721297
381 381 -, ctc dbSNP:769603439
381 381 c, t dbSNP:373247834
383 383 c, t dbSNP:41302158
389 389 c, g, t dbSNP:148498181
390 390 -, ctc dbSNP:372640194
390 390 c, g dbSNP:377034330
394 394 c, g dbSNP:201754404
397 397 c, t dbSNP:776532944
402 402 c, t dbSNP:759371757
408 408 c, t dbSNP:765279987
411 411 c, t dbSNP:752682980
416 416 a, t dbSNP:757344806
418 418 a, g dbSNP:767725718
420 420 a, c dbSNP:750630461
427 427 c, g dbSNP:370000858
434 434 a, g dbSNP:146569098
440 440 c, t dbSNP:780257307
464 464 a, g dbSNP:199930521
468 468 c, t dbSNP:758986419
475 475 a, t dbSNP:779325818
477 477 -, c dbSNP:34118273
477 477 a, c dbSNP:748667370
479 479 c, g, t dbSNP:763607469
480 480 c, g dbSNP:746613627
489 489 c, t dbSNP:770669132
492 492 c, t dbSNP:374428401
496 496 a, g dbSNP:759446643
497 497 c, g dbSNP:764951617
515 515 a, g dbSNP:377413340
516 516 a, t dbSNP:763013442
522 522 c, t dbSNP:767670794
526 526 a, g dbSNP:750579206
534 534 a, c dbSNP:756275786
540 540 g, t dbSNP:766690180
544 544 a, g dbSNP:754011082
547 547 c, t dbSNP:755313244
549 549 a, g dbSNP:201418399
553 553 a, g dbSNP:373757058
557 557 c, g, t dbSNP:200774631
558 558 a, g dbSNP:761277562
560 560 c, t dbSNP:140138379
565 565 a, g dbSNP:770490747
569 569 a, c dbSNP:776285619
576 576 c, g dbSNP:778217233
580 580 a, c dbSNP:373393448
581 581 a, g dbSNP:769670092
586 586 a, g dbSNP:775463116
597 597 c, g, t dbSNP:199755286
616 616 c, t dbSNP:773420574
621 621 c, t dbSNP:747399608
624 624 g, t dbSNP:766382651
628 628 c, t dbSNP:143837393
629 629 a, g dbSNP:759710688
637 637 a, c, g dbSNP:765557256
638 638 a, g dbSNP:151294389
648 648 a, g dbSNP:766749023
650 650 c, t dbSNP:752017073
656 656 c, g dbSNP:771228306
661 661 a, g dbSNP:181401508
678 678 a, c, t dbSNP:151060324
680 680 c, t dbSNP:745500251
684 684 c, g dbSNP:769617094
685 685 a, g dbSNP:779980180
686 686 a, g dbSNP:749161613
697 697 c, t dbSNP:768575994
698 698 a, g, t dbSNP:200822743
706 706 a, c dbSNP:771004302
708 708 c, t dbSNP:776616211
710 710 a, t dbSNP:759733983
717 717 c, t dbSNP:765504023
718 718 a, g dbSNP:200025917
722 722 a, g dbSNP:745602686
725 725 c, t dbSNP:763240762
729 729 c, t dbSNP:202047929
733 733 g, t dbSNP:752062577
734 734 c, g dbSNP:772388956
744 744 g, t dbSNP:757742313
759 759 c, t dbSNP:376596001
760 760 a, g, t dbSNP:778001194
770 770 c, t dbSNP:755823543
771 771 a, g dbSNP:779785942
777 777 c, t dbSNP:200853372
778 778 a, g dbSNP:1047251
781 781 a, g dbSNP:778605916
790 790 c, t dbSNP:748090014
794 794 c, t dbSNP:61743726
801 801 c, t dbSNP:776762269
803 803 c, t dbSNP:746017699
804 804 c, t dbSNP:781034122
807 807 c, t dbSNP:775637049
821 821 a, c, g, t dbSNP:199657597
828 828 c, t dbSNP:762366485
832 832 c, g dbSNP:141071979
834 834 a, g dbSNP:750943985
837 837 -, taa dbSNP:748977026
849 849 c, t dbSNP:755627386
861 861 a, g dbSNP:61741057
874 874 a, g dbSNP:753502144
876 876 c, t dbSNP:754718950
879 879 c, t dbSNP:778747927
886 886 a, t dbSNP:747954097
903 903 c, t dbSNP:376198310
904 904 a, g dbSNP:371047147
909 909 c, t dbSNP:61746135
910 910 a, g dbSNP:374430540
911 911 c, t dbSNP:775207650
927 927 a, c dbSNP:143502348
934 934 c, g dbSNP:143158098
940 940 g, t dbSNP:749487190
943 943 a, g dbSNP:768816689
949 949 a, g dbSNP:768259232
950 950 a, g dbSNP:773875619
951 951 c, g, t dbSNP:141361139
952 952 c, g, t dbSNP:150785634
954 954 a, g dbSNP:753449158
959 959 a, t dbSNP:754647855
965 965 g, t dbSNP:572691118
974 974 c, g dbSNP:139539716
979 979 c, t dbSNP:142730380
985 985 a, g dbSNP:777650849
996 996 a, g dbSNP:200230138
1000 1000 c, g dbSNP:767351911
1004 1004 c, t dbSNP:757230613
1011 1011 g, t dbSNP:146055714
1022 1022 a, g dbSNP:749434238
1023 1023 a, g dbSNP:140091480
1029 1029 c, t dbSNP:149797451
1037 1037 a, g dbSNP:748423582
1039 1039 c, t dbSNP:772283795
1040 1040 a, g dbSNP:145834905
1045 1045 a, g dbSNP:761242053
1046 1046 g, t dbSNP:771624598
1047 1047 a, g dbSNP:758722494
1050 1050 c, t dbSNP:199984362
1051 1051 a, g dbSNP:148549437
1053 1053 c, g dbSNP:752338067
1054 1054 a, g dbSNP:762630453
1057 1057 a, g dbSNP:200666877
1060 1060 c, t dbSNP:201017700
1064 1064 c, t dbSNP:190319017
1065 1065 a, g, t dbSNP:78850862
1066 1066 c, t dbSNP:755062941
1067 1067 a, g dbSNP:151108304
1070 1070 a, g dbSNP:141140102
1071 1071 c, t dbSNP:772288131
1078 1078 c, t dbSNP:778294490
1089 1089 a, g dbSNP:747308965
1091 1091 a, g dbSNP:771415744
1095 1095 c, t dbSNP:779856959
1098 1098 g, t dbSNP:200409709
1100 1100 c, g dbSNP:769223687
1109 1109 c, g dbSNP:775020136
1112 1112 a, g dbSNP:762530888
1123 1123 c, t dbSNP:763672752
1128 1128 a, g dbSNP:751211785
1135 1135 a, g dbSNP:367779834
1140 1140 g, t dbSNP:767279676
1141 1141 a, g dbSNP:750367920
1145 1145 a, g dbSNP:768419102
1159 1159 a, g dbSNP:778880264
1162 1162 g, t dbSNP:752763771
1166 1166 a, t dbSNP:199759051
1173 1173 c, t dbSNP:146939572
1174 1174 c, t dbSNP:541767318
1177 1177 c, t dbSNP:747218857
1178 1178 a, g dbSNP:757742093
1191 1191 c, g dbSNP:781659856
1195 1195 c, g dbSNP:746388689
1201 1201 a, g dbSNP:147562044
1207 1207 a, g dbSNP:142110631
1210 1210 c, g dbSNP:748897879
1213 1213 -, tg dbSNP:768537377
1220 1220 c, g dbSNP:768143061
1223 1223 c, t dbSNP:774040894
1229 1229 c, t dbSNP:761425574
1238 1238 c, t dbSNP:371304448
1243 1243 a, g dbSNP:773010399
1254 1254 c, g dbSNP:760542697
1266 1266 a, g dbSNP:766271224
1274 1274 g, t dbSNP:752857813
1301 1301 a, g dbSNP:147110981
1308 1308 -, atg dbSNP:759964480
1342 1342 -, agtc dbSNP:765798798
1395 1395 c, t dbSNP:372500407
1428 1428 c, t dbSNP:763457248
1451 1451 c, g dbSNP:191084574
1475 1475 a, g dbSNP:370556419
1483 1483 c, t dbSNP:778266553
1484 1484 a, g dbSNP:376774987
1494 1494 a, g dbSNP:527276181
1517 1517 a, g dbSNP:757443950
1547 1547 a, g dbSNP:767504019
1550 1550 a, c dbSNP:182183554
1570 1570 -, ata dbSNP:750367443
1575 1575 -, aac dbSNP:748069999
1623 1623 a, g dbSNP:751997214
1651 1651 c, g dbSNP:763916323
1664 1664 c, t dbSNP:186128975
1671 1671 c, t dbSNP:749191218
1673 1673 c, t dbSNP:549311870
1679 1679 a, g dbSNP:191003019
1683 1683 -, a dbSNP:778751966
1688 1688 a, t dbSNP:747765671
1719 1719 c, t dbSNP:757758095
1764 1764 g, t dbSNP:781596393

Target ORF information:

RefSeq Version XM_005274677
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens melanoma antigen family A4 (MAGEA4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005274677

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCCACTGA CTTGCGCATT GGGGGTTAGA GAGAAGCGAG CTGCTCTGAC CAGCCGCTTG 
GGATTGGCGG AGGGAAGCGG GCCAGGCCCT GTGAGGAGTC AAGGTTCTGA GCAGACAGGC
CAACCGGAGG ACAGGATTCC CTGGAGGCCA CAGAGGAGCA CCAAGGAGAA GATCTGCCTG
TGGGTCCCCA TTGCCCAGCT TTTGCCTGCA CTCTTGCCTG CTGCCCTGAC CAGAGTCATC
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu02154D
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011681.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530422792. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005274736.1
CDS230..1183
Misc Feature(1)236..526(+)
Misc Feature(2)578..1090(+)
Translation
Position Chain Variation Link
8 8 c, g dbSNP:774118615
20 20 a, g dbSNP:761468637
50 50 c, g dbSNP:771645052
57 57 c, t dbSNP:2856381
96 96 a, c, t dbSNP:745816060
103 103 a, g dbSNP:774445671
108 108 a, g dbSNP:748368449
112 112 a, g dbSNP:368738694
113 113 c, t dbSNP:773613926
114 114 c, t dbSNP:763594807
115 115 a, g dbSNP:766855228
130 130 c, g dbSNP:777222146
139 139 a, g dbSNP:148460606
140 140 c, t dbSNP:764802119
154 154 g, t dbSNP:752191271
156 156 -, aga dbSNP:745310597
163 163 c, t dbSNP:758051896
166 166 c, t dbSNP:775056875
174 174 -, c dbSNP:769356179
175 175 c, t dbSNP:762298297
178 178 c, t dbSNP:183961725
179 179 a, g dbSNP:144129975
180 180 a, t dbSNP:761483501
187 187 a, g dbSNP:372125339
200 200 c, g dbSNP:767267374
201 201 c, t dbSNP:750267906
207 207 c, t dbSNP:756031183
212 212 a, g dbSNP:779923857
219 219 c, g dbSNP:1047246
227 227 a, g dbSNP:201648066
234 234 c, t dbSNP:777932203
240 240 a, g dbSNP:747163064
245 245 a, g dbSNP:771323726
246 246 a, g dbSNP:781676215
253 253 a, g dbSNP:746298946
257 257 c, t dbSNP:770283475
258 258 a, g dbSNP:775799958
259 259 c, t dbSNP:763535308
274 274 c, t dbSNP:200225562
275 275 a, g dbSNP:142773415
276 276 c, t dbSNP:780727589
284 284 c, g dbSNP:147367105
293 293 g, t dbSNP:767214466
294 294 c, t dbSNP:750215141
299 299 a, g dbSNP:760396181
303 303 c, t dbSNP:766211889
309 309 a, g, t dbSNP:753670573
310 310 c, t dbSNP:777687166
312 312 c, t dbSNP:772345662
313 313 a, g dbSNP:1047248
316 316 a, g dbSNP:150592561
318 318 c, t dbSNP:746953197
320 320 c, t dbSNP:61743686
326 326 a, t dbSNP:149323734
329 329 a, g dbSNP:749725686
342 342 c, t dbSNP:759301904
343 343 c, t dbSNP:369838724
348 348 a, t dbSNP:773721297
349 349 -, ctc dbSNP:769603439
349 349 c, t dbSNP:373247834
351 351 c, t dbSNP:41302158
357 357 c, g, t dbSNP:148498181
358 358 -, ctc dbSNP:372640194
358 358 c, g dbSNP:377034330
362 362 c, g dbSNP:201754404
365 365 c, t dbSNP:776532944
370 370 c, t dbSNP:759371757
376 376 c, t dbSNP:765279987
379 379 c, t dbSNP:752682980
384 384 a, t dbSNP:757344806
386 386 a, g dbSNP:767725718
388 388 a, c dbSNP:750630461
395 395 c, g dbSNP:370000858
402 402 a, g dbSNP:146569098
408 408 c, t dbSNP:780257307
432 432 a, g dbSNP:199930521
436 436 c, t dbSNP:758986419
443 443 a, t dbSNP:779325818
445 445 -, c dbSNP:34118273
445 445 a, c dbSNP:748667370
447 447 c, g, t dbSNP:763607469
448 448 c, g dbSNP:746613627
457 457 c, t dbSNP:770669132
460 460 c, t dbSNP:374428401
464 464 a, g dbSNP:759446643
465 465 c, g dbSNP:764951617
483 483 a, g dbSNP:377413340
484 484 a, t dbSNP:763013442
490 490 c, t dbSNP:767670794
494 494 a, g dbSNP:750579206
502 502 a, c dbSNP:756275786
508 508 g, t dbSNP:766690180
512 512 a, g dbSNP:754011082
515 515 c, t dbSNP:755313244
517 517 a, g dbSNP:201418399
521 521 a, g dbSNP:373757058
525 525 c, g, t dbSNP:200774631
526 526 a, g dbSNP:761277562
528 528 c, t dbSNP:140138379
533 533 a, g dbSNP:770490747
537 537 a, c dbSNP:776285619
544 544 c, g dbSNP:778217233
548 548 a, c dbSNP:373393448
549 549 a, g dbSNP:769670092
554 554 a, g dbSNP:775463116
565 565 c, g, t dbSNP:199755286
584 584 c, t dbSNP:773420574
589 589 c, t dbSNP:747399608
592 592 g, t dbSNP:766382651
596 596 c, t dbSNP:143837393
597 597 a, g dbSNP:759710688
605 605 a, c, g dbSNP:765557256
606 606 a, g dbSNP:151294389
616 616 a, g dbSNP:766749023
618 618 c, t dbSNP:752017073
624 624 c, g dbSNP:771228306
629 629 a, g dbSNP:181401508
646 646 a, c, t dbSNP:151060324
648 648 c, t dbSNP:745500251
652 652 c, g dbSNP:769617094
653 653 a, g dbSNP:779980180
654 654 a, g dbSNP:749161613
665 665 c, t dbSNP:768575994
666 666 a, g, t dbSNP:200822743
674 674 a, c dbSNP:771004302
676 676 c, t dbSNP:776616211
678 678 a, t dbSNP:759733983
685 685 c, t dbSNP:765504023
686 686 a, g dbSNP:200025917
690 690 a, g dbSNP:745602686
693 693 c, t dbSNP:763240762
697 697 c, t dbSNP:202047929
701 701 g, t dbSNP:752062577
702 702 c, g dbSNP:772388956
712 712 g, t dbSNP:757742313
727 727 c, t dbSNP:376596001
728 728 a, g, t dbSNP:778001194
738 738 c, t dbSNP:755823543
739 739 a, g dbSNP:779785942
745 745 c, t dbSNP:200853372
746 746 a, g dbSNP:1047251
749 749 a, g dbSNP:778605916
758 758 c, t dbSNP:748090014
762 762 c, t dbSNP:61743726
769 769 c, t dbSNP:776762269
771 771 c, t dbSNP:746017699
772 772 c, t dbSNP:781034122
775 775 c, t dbSNP:775637049
789 789 a, c, g, t dbSNP:199657597
796 796 c, t dbSNP:762366485
800 800 c, g dbSNP:141071979
802 802 a, g dbSNP:750943985
805 805 -, taa dbSNP:748977026
817 817 c, t dbSNP:755627386
829 829 a, g dbSNP:61741057
842 842 a, g dbSNP:753502144
844 844 c, t dbSNP:754718950
847 847 c, t dbSNP:778747927
854 854 a, t dbSNP:747954097
871 871 c, t dbSNP:376198310
872 872 a, g dbSNP:371047147
877 877 c, t dbSNP:61746135
878 878 a, g dbSNP:374430540
879 879 c, t dbSNP:775207650
895 895 a, c dbSNP:143502348
902 902 c, g dbSNP:143158098
908 908 g, t dbSNP:749487190
911 911 a, g dbSNP:768816689
917 917 a, g dbSNP:768259232
918 918 a, g dbSNP:773875619
919 919 c, g, t dbSNP:141361139
920 920 c, g, t dbSNP:150785634
922 922 a, g dbSNP:753449158
927 927 a, t dbSNP:754647855
933 933 g, t dbSNP:572691118
942 942 c, g dbSNP:139539716
947 947 c, t dbSNP:142730380
953 953 a, g dbSNP:777650849
964 964 a, g dbSNP:200230138
968 968 c, g dbSNP:767351911
972 972 c, t dbSNP:757230613
979 979 g, t dbSNP:146055714
990 990 a, g dbSNP:749434238
991 991 a, g dbSNP:140091480
997 997 c, t dbSNP:149797451
1005 1005 a, g dbSNP:748423582
1007 1007 c, t dbSNP:772283795
1008 1008 a, g dbSNP:145834905
1013 1013 a, g dbSNP:761242053
1014 1014 g, t dbSNP:771624598
1015 1015 a, g dbSNP:758722494
1018 1018 c, t dbSNP:199984362
1019 1019 a, g dbSNP:148549437
1021 1021 c, g dbSNP:752338067
1022 1022 a, g dbSNP:762630453
1025 1025 a, g dbSNP:200666877
1028 1028 c, t dbSNP:201017700
1032 1032 c, t dbSNP:190319017
1033 1033 a, g, t dbSNP:78850862
1034 1034 c, t dbSNP:755062941
1035 1035 a, g dbSNP:151108304
1038 1038 a, g dbSNP:141140102
1039 1039 c, t dbSNP:772288131
1046 1046 c, t dbSNP:778294490
1057 1057 a, g dbSNP:747308965
1059 1059 a, g dbSNP:771415744
1063 1063 c, t dbSNP:779856959
1066 1066 g, t dbSNP:200409709
1068 1068 c, g dbSNP:769223687
1077 1077 c, g dbSNP:775020136
1080 1080 a, g dbSNP:762530888
1091 1091 c, t dbSNP:763672752
1096 1096 a, g dbSNP:751211785
1103 1103 a, g dbSNP:367779834
1108 1108 g, t dbSNP:767279676
1109 1109 a, g dbSNP:750367920
1113 1113 a, g dbSNP:768419102
1127 1127 a, g dbSNP:778880264
1130 1130 g, t dbSNP:752763771
1134 1134 a, t dbSNP:199759051
1141 1141 c, t dbSNP:146939572
1142 1142 c, t dbSNP:541767318
1145 1145 c, t dbSNP:747218857
1146 1146 a, g dbSNP:757742093
1159 1159 c, g dbSNP:781659856
1163 1163 c, g dbSNP:746388689
1169 1169 a, g dbSNP:147562044
1175 1175 a, g dbSNP:142110631
1178 1178 c, g dbSNP:748897879
1181 1181 -, tg dbSNP:768537377
1188 1188 c, g dbSNP:768143061
1191 1191 c, t dbSNP:774040894
1197 1197 c, t dbSNP:761425574
1206 1206 c, t dbSNP:371304448
1211 1211 a, g dbSNP:773010399
1222 1222 c, g dbSNP:760542697
1234 1234 a, g dbSNP:766271224
1242 1242 g, t dbSNP:752857813
1269 1269 a, g dbSNP:147110981
1276 1276 -, atg dbSNP:759964480
1310 1310 -, agtc dbSNP:765798798
1363 1363 c, t dbSNP:372500407
1396 1396 c, t dbSNP:763457248
1419 1419 c, g dbSNP:191084574
1443 1443 a, g dbSNP:370556419
1451 1451 c, t dbSNP:778266553
1452 1452 a, g dbSNP:376774987
1462 1462 a, g dbSNP:527276181
1485 1485 a, g dbSNP:757443950
1515 1515 a, g dbSNP:767504019
1518 1518 a, c dbSNP:182183554
1538 1538 -, ata dbSNP:750367443
1543 1543 -, aac dbSNP:748069999
1591 1591 a, g dbSNP:751997214
1619 1619 c, g dbSNP:763916323
1632 1632 c, t dbSNP:186128975
1639 1639 c, t dbSNP:749191218
1641 1641 c, t dbSNP:549311870
1647 1647 a, g dbSNP:191003019
1651 1651 -, a dbSNP:778751966
1656 1656 a, t dbSNP:747765671
1687 1687 c, t dbSNP:757758095
1732 1732 g, t dbSNP:781596393

Target ORF information:

RefSeq Version XM_005274679
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens melanoma antigen family A4 (MAGEA4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005274679

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu02154D
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011681.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578838849. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005274735.1
CDS220..1173
Misc Feature(1)226..516(+)
Misc Feature(2)568..1080(+)
Translation
Position Chain Variation Link
13 13 a, g dbSNP:764660830
18 18 c, g dbSNP:774882003
22 22 a, t dbSNP:375813460
73 73 a, g dbSNP:762317238
86 86 a, c, t dbSNP:745816060
93 93 a, g dbSNP:774445671
98 98 a, g dbSNP:748368449
102 102 a, g dbSNP:368738694
103 103 c, t dbSNP:773613926
104 104 c, t dbSNP:763594807
105 105 a, g dbSNP:766855228
120 120 c, g dbSNP:777222146
129 129 a, g dbSNP:148460606
130 130 c, t dbSNP:764802119
144 144 g, t dbSNP:752191271
146 146 -, aga dbSNP:745310597
153 153 c, t dbSNP:758051896
156 156 c, t dbSNP:775056875
164 164 -, c dbSNP:769356179
165 165 c, t dbSNP:762298297
168 168 c, t dbSNP:183961725
169 169 a, g dbSNP:144129975
170 170 a, t dbSNP:761483501
177 177 a, g dbSNP:372125339
190 190 c, g dbSNP:767267374
191 191 c, t dbSNP:750267906
197 197 c, t dbSNP:756031183
202 202 a, g dbSNP:779923857
209 209 c, g dbSNP:1047246
217 217 a, g dbSNP:201648066
224 224 c, t dbSNP:777932203
230 230 a, g dbSNP:747163064
235 235 a, g dbSNP:771323726
236 236 a, g dbSNP:781676215
243 243 a, g dbSNP:746298946
247 247 c, t dbSNP:770283475
248 248 a, g dbSNP:775799958
249 249 c, t dbSNP:763535308
264 264 c, t dbSNP:200225562
265 265 a, g dbSNP:142773415
266 266 c, t dbSNP:780727589
274 274 c, g dbSNP:147367105
283 283 g, t dbSNP:767214466
284 284 c, t dbSNP:750215141
289 289 a, g dbSNP:760396181
293 293 c, t dbSNP:766211889
299 299 a, g, t dbSNP:753670573
300 300 c, t dbSNP:777687166
302 302 c, t dbSNP:772345662
303 303 a, g dbSNP:1047248
306 306 a, g dbSNP:150592561
308 308 c, t dbSNP:746953197
310 310 c, t dbSNP:61743686
316 316 a, t dbSNP:149323734
319 319 a, g dbSNP:749725686
332 332 c, t dbSNP:759301904
333 333 c, t dbSNP:369838724
338 338 a, t dbSNP:773721297
339 339 -, ctc dbSNP:769603439
339 339 c, t dbSNP:373247834
341 341 c, t dbSNP:41302158
347 347 c, g, t dbSNP:148498181
348 348 -, ctc dbSNP:372640194
348 348 c, g dbSNP:377034330
352 352 c, g dbSNP:201754404
355 355 c, t dbSNP:776532944
360 360 c, t dbSNP:759371757
366 366 c, t dbSNP:765279987
369 369 c, t dbSNP:752682980
374 374 a, t dbSNP:757344806
376 376 a, g dbSNP:767725718
378 378 a, c dbSNP:750630461
385 385 c, g dbSNP:370000858
392 392 a, g dbSNP:146569098
398 398 c, t dbSNP:780257307
422 422 a, g dbSNP:199930521
426 426 c, t dbSNP:758986419
433 433 a, t dbSNP:779325818
435 435 -, c dbSNP:34118273
435 435 a, c dbSNP:748667370
437 437 c, g, t dbSNP:763607469
438 438 c, g dbSNP:746613627
447 447 c, t dbSNP:770669132
450 450 c, t dbSNP:374428401
454 454 a, g dbSNP:759446643
455 455 c, g dbSNP:764951617
473 473 a, g dbSNP:377413340
474 474 a, t dbSNP:763013442
480 480 c, t dbSNP:767670794
484 484 a, g dbSNP:750579206
492 492 a, c dbSNP:756275786
498 498 g, t dbSNP:766690180
502 502 a, g dbSNP:754011082
505 505 c, t dbSNP:755313244
507 507 a, g dbSNP:201418399
511 511 a, g dbSNP:373757058
515 515 c, g, t dbSNP:200774631
516 516 a, g dbSNP:761277562
518 518 c, t dbSNP:140138379
523 523 a, g dbSNP:770490747
527 527 a, c dbSNP:776285619
534 534 c, g dbSNP:778217233
538 538 a, c dbSNP:373393448
539 539 a, g dbSNP:769670092
544 544 a, g dbSNP:775463116
555 555 c, g, t dbSNP:199755286
574 574 c, t dbSNP:773420574
579 579 c, t dbSNP:747399608
582 582 g, t dbSNP:766382651
586 586 c, t dbSNP:143837393
587 587 a, g dbSNP:759710688
595 595 a, c, g dbSNP:765557256
596 596 a, g dbSNP:151294389
606 606 a, g dbSNP:766749023
608 608 c, t dbSNP:752017073
614 614 c, g dbSNP:771228306
619 619 a, g dbSNP:181401508
636 636 a, c, t dbSNP:151060324
638 638 c, t dbSNP:745500251
642 642 c, g dbSNP:769617094
643 643 a, g dbSNP:779980180
644 644 a, g dbSNP:749161613
655 655 c, t dbSNP:768575994
656 656 a, g, t dbSNP:200822743
664 664 a, c dbSNP:771004302
666 666 c, t dbSNP:776616211
668 668 a, t dbSNP:759733983
675 675 c, t dbSNP:765504023
676 676 a, g dbSNP:200025917
680 680 a, g dbSNP:745602686
683 683 c, t dbSNP:763240762
687 687 c, t dbSNP:202047929
691 691 g, t dbSNP:752062577
692 692 c, g dbSNP:772388956
702 702 g, t dbSNP:757742313
717 717 c, t dbSNP:376596001
718 718 a, g, t dbSNP:778001194
728 728 c, t dbSNP:755823543
729 729 a, g dbSNP:779785942
735 735 c, t dbSNP:200853372
736 736 a, g dbSNP:1047251
739 739 a, g dbSNP:778605916
748 748 c, t dbSNP:748090014
752 752 c, t dbSNP:61743726
759 759 c, t dbSNP:776762269
761 761 c, t dbSNP:746017699
762 762 c, t dbSNP:781034122
765 765 c, t dbSNP:775637049
779 779 a, c, g, t dbSNP:199657597
786 786 c, t dbSNP:762366485
790 790 c, g dbSNP:141071979
792 792 a, g dbSNP:750943985
795 795 -, taa dbSNP:748977026
807 807 c, t dbSNP:755627386
819 819 a, g dbSNP:61741057
832 832 a, g dbSNP:753502144
834 834 c, t dbSNP:754718950
837 837 c, t dbSNP:778747927
844 844 a, t dbSNP:747954097
861 861 c, t dbSNP:376198310
862 862 a, g dbSNP:371047147
867 867 c, t dbSNP:61746135
868 868 a, g dbSNP:374430540
869 869 c, t dbSNP:775207650
885 885 a, c dbSNP:143502348
892 892 c, g dbSNP:143158098
898 898 g, t dbSNP:749487190
901 901 a, g dbSNP:768816689
907 907 a, g dbSNP:768259232
908 908 a, g dbSNP:773875619
909 909 c, g, t dbSNP:141361139
910 910 c, g, t dbSNP:150785634
912 912 a, g dbSNP:753449158
917 917 a, t dbSNP:754647855
923 923 g, t dbSNP:572691118
932 932 c, g dbSNP:139539716
937 937 c, t dbSNP:142730380
943 943 a, g dbSNP:777650849
954 954 a, g dbSNP:200230138
958 958 c, g dbSNP:767351911
962 962 c, t dbSNP:757230613
969 969 g, t dbSNP:146055714
980 980 a, g dbSNP:749434238
981 981 a, g dbSNP:140091480
987 987 c, t dbSNP:149797451
995 995 a, g dbSNP:748423582
997 997 c, t dbSNP:772283795
998 998 a, g dbSNP:145834905
1003 1003 a, g dbSNP:761242053
1004 1004 g, t dbSNP:771624598
1005 1005 a, g dbSNP:758722494
1008 1008 c, t dbSNP:199984362
1009 1009 a, g dbSNP:148549437
1011 1011 c, g dbSNP:752338067
1012 1012 a, g dbSNP:762630453
1015 1015 a, g dbSNP:200666877
1018 1018 c, t dbSNP:201017700
1022 1022 c, t dbSNP:190319017
1023 1023 a, g, t dbSNP:78850862
1024 1024 c, t dbSNP:755062941
1025 1025 a, g dbSNP:151108304
1028 1028 a, g dbSNP:141140102
1029 1029 c, t dbSNP:772288131
1036 1036 c, t dbSNP:778294490
1047 1047 a, g dbSNP:747308965
1049 1049 a, g dbSNP:771415744
1053 1053 c, t dbSNP:779856959
1056 1056 g, t dbSNP:200409709
1058 1058 c, g dbSNP:769223687
1067 1067 c, g dbSNP:775020136
1070 1070 a, g dbSNP:762530888
1081 1081 c, t dbSNP:763672752
1086 1086 a, g dbSNP:751211785
1093 1093 a, g dbSNP:367779834
1098 1098 g, t dbSNP:767279676
1099 1099 a, g dbSNP:750367920
1103 1103 a, g dbSNP:768419102
1117 1117 a, g dbSNP:778880264
1120 1120 g, t dbSNP:752763771
1124 1124 a, t dbSNP:199759051
1131 1131 c, t dbSNP:146939572
1132 1132 c, t dbSNP:541767318
1135 1135 c, t dbSNP:747218857
1136 1136 a, g dbSNP:757742093
1149 1149 c, g dbSNP:781659856
1153 1153 c, g dbSNP:746388689
1159 1159 a, g dbSNP:147562044
1165 1165 a, g dbSNP:142110631
1168 1168 c, g dbSNP:748897879
1171 1171 -, tg dbSNP:768537377
1178 1178 c, g dbSNP:768143061
1181 1181 c, t dbSNP:774040894
1187 1187 c, t dbSNP:761425574
1196 1196 c, t dbSNP:371304448
1201 1201 a, g dbSNP:773010399
1212 1212 c, g dbSNP:760542697
1224 1224 a, g dbSNP:766271224
1232 1232 g, t dbSNP:752857813
1259 1259 a, g dbSNP:147110981
1266 1266 -, atg dbSNP:759964480
1300 1300 -, agtc dbSNP:765798798
1353 1353 c, t dbSNP:372500407
1386 1386 c, t dbSNP:763457248
1409 1409 c, g dbSNP:191084574
1433 1433 a, g dbSNP:370556419
1441 1441 c, t dbSNP:778266553
1442 1442 a, g dbSNP:376774987
1452 1452 a, g dbSNP:527276181
1475 1475 a, g dbSNP:757443950
1505 1505 a, g dbSNP:767504019
1508 1508 a, c dbSNP:182183554
1528 1528 -, ata dbSNP:750367443
1533 1533 -, aac dbSNP:748069999
1581 1581 a, g dbSNP:751997214
1609 1609 c, g dbSNP:763916323
1622 1622 c, t dbSNP:186128975
1629 1629 c, t dbSNP:749191218
1631 1631 c, t dbSNP:549311870
1637 1637 a, g dbSNP:191003019
1641 1641 -, a dbSNP:778751966
1646 1646 a, t dbSNP:747765671
1677 1677 c, t dbSNP:757758095
1722 1722 g, t dbSNP:781596393

Target ORF information:

RefSeq Version XM_005274678
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens melanoma antigen family A4 (MAGEA4), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005274678

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu02154D
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM562570.1 and AF274855.3. On Feb 2, 2005 this sequence version replaced gi:29029623. Summary: This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All four variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM548890.1, BX337705.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_002353.3
CDS222..1175
Misc Feature(1)228..518(+)
Misc Feature(2)570..1082(+)
Exon (1)1..84
Gene:MAGEA4
Gene Synonym:
Exon (2)85..156
Gene:MAGEA4
Gene Synonym:
Exon (3)157..1727
Gene:MAGEA4
Gene Synonym:
Translation
Position Chain Variation Link
88 88 a, c, t dbSNP:745816060
95 95 a, g dbSNP:774445671
100 100 a, g dbSNP:748368449
104 104 a, g dbSNP:368738694
105 105 c, t dbSNP:773613926
106 106 c, t dbSNP:763594807
107 107 a, g dbSNP:766855228
122 122 c, g dbSNP:777222146
131 131 a, g dbSNP:148460606
132 132 c, t dbSNP:764802119
146 146 g, t dbSNP:752191271
148 148 -, aga dbSNP:745310597
155 155 c, t dbSNP:758051896
158 158 c, t dbSNP:775056875
166 166 -, c dbSNP:769356179
167 167 c, t dbSNP:762298297
170 170 c, t dbSNP:183961725
171 171 a, g dbSNP:144129975
172 172 a, t dbSNP:761483501
179 179 a, g dbSNP:372125339
192 192 c, g dbSNP:767267374
193 193 c, t dbSNP:750267906
199 199 c, t dbSNP:756031183
204 204 a, g dbSNP:779923857
211 211 c, g dbSNP:1047246
219 219 a, g dbSNP:201648066
226 226 c, t dbSNP:777932203
232 232 a, g dbSNP:747163064
237 237 a, g dbSNP:771323726
238 238 a, g dbSNP:781676215
245 245 a, g dbSNP:746298946
249 249 c, t dbSNP:770283475
250 250 a, g dbSNP:775799958
251 251 c, t dbSNP:763535308
266 266 c, t dbSNP:200225562
267 267 a, g dbSNP:142773415
268 268 c, t dbSNP:780727589
276 276 c, g dbSNP:147367105
285 285 g, t dbSNP:767214466
286 286 c, t dbSNP:750215141
291 291 a, g dbSNP:760396181
295 295 c, t dbSNP:766211889
301 301 a, g, t dbSNP:753670573
302 302 c, t dbSNP:777687166
304 304 c, t dbSNP:772345662
305 305 a, g dbSNP:1047248
308 308 a, g dbSNP:150592561
310 310 c, t dbSNP:746953197
312 312 c, t dbSNP:61743686
318 318 a, t dbSNP:149323734
321 321 a, g dbSNP:749725686
334 334 c, t dbSNP:759301904
335 335 c, t dbSNP:369838724
340 340 a, t dbSNP:773721297
341 341 -, ctc dbSNP:769603439
341 341 c, t dbSNP:373247834
343 343 c, t dbSNP:41302158
349 349 c, g, t dbSNP:148498181
350 350 -, ctc dbSNP:372640194
350 350 c, g dbSNP:377034330
354 354 c, g dbSNP:201754404
357 357 c, t dbSNP:776532944
362 362 c, t dbSNP:759371757
368 368 c, t dbSNP:765279987
371 371 c, t dbSNP:752682980
376 376 a, t dbSNP:757344806
378 378 a, g dbSNP:767725718
380 380 a, c dbSNP:750630461
387 387 c, g dbSNP:370000858
394 394 a, g dbSNP:146569098
400 400 c, t dbSNP:780257307
424 424 a, g dbSNP:199930521
428 428 c, t dbSNP:758986419
435 435 a, t dbSNP:779325818
437 437 -, c dbSNP:34118273
437 437 a, c dbSNP:748667370
439 439 c, g, t dbSNP:763607469
440 440 c, g dbSNP:746613627
449 449 c, t dbSNP:770669132
452 452 c, t dbSNP:374428401
456 456 a, g dbSNP:759446643
457 457 c, g dbSNP:764951617
475 475 a, g dbSNP:377413340
476 476 a, t dbSNP:763013442
482 482 c, t dbSNP:767670794
486 486 a, g dbSNP:750579206
494 494 a, c dbSNP:756275786
500 500 g, t dbSNP:766690180
504 504 a, g dbSNP:754011082
507 507 c, t dbSNP:755313244
509 509 a, g dbSNP:201418399
513 513 a, g dbSNP:373757058
517 517 c, g, t dbSNP:200774631
518 518 a, g dbSNP:761277562
520 520 c, t dbSNP:140138379
525 525 a, g dbSNP:770490747
529 529 a, c dbSNP:776285619
536 536 c, g dbSNP:778217233
540 540 a, c dbSNP:373393448
541 541 a, g dbSNP:769670092
546 546 a, g dbSNP:775463116
557 557 c, g, t dbSNP:199755286
576 576 c, t dbSNP:773420574
581 581 c, t dbSNP:747399608
584 584 g, t dbSNP:766382651
588 588 c, t dbSNP:143837393
589 589 a, g dbSNP:759710688
597 597 a, c, g dbSNP:765557256
598 598 a, g dbSNP:151294389
608 608 a, g dbSNP:766749023
610 610 c, t dbSNP:752017073
616 616 c, g dbSNP:771228306
621 621 a, g dbSNP:181401508
638 638 a, c, t dbSNP:151060324
640 640 c, t dbSNP:745500251
644 644 c, g dbSNP:769617094
645 645 a, g dbSNP:779980180
646 646 a, g dbSNP:749161613
657 657 c, t dbSNP:768575994
658 658 a, g, t dbSNP:200822743
666 666 a, c dbSNP:771004302
668 668 c, t dbSNP:776616211
670 670 a, t dbSNP:759733983
677 677 c, t dbSNP:765504023
678 678 a, g dbSNP:200025917
682 682 a, g dbSNP:745602686
685 685 c, t dbSNP:763240762
689 689 c, t dbSNP:202047929
693 693 g, t dbSNP:752062577
694 694 c, g dbSNP:772388956
704 704 g, t dbSNP:757742313
719 719 c, t dbSNP:376596001
720 720 a, g, t dbSNP:778001194
730 730 c, t dbSNP:755823543
731 731 a, g dbSNP:779785942
737 737 c, t dbSNP:200853372
738 738 a, g dbSNP:1047251
741 741 a, g dbSNP:778605916
750 750 c, t dbSNP:748090014
754 754 c, t dbSNP:61743726
761 761 c, t dbSNP:776762269
763 763 c, t dbSNP:746017699
764 764 c, t dbSNP:781034122
767 767 c, t dbSNP:775637049
781 781 a, c, g, t dbSNP:199657597
788 788 c, t dbSNP:762366485
792 792 c, g dbSNP:141071979
794 794 a, g dbSNP:750943985
797 797 -, taa dbSNP:748977026
809 809 c, t dbSNP:755627386
821 821 a, g dbSNP:61741057
834 834 a, g dbSNP:753502144
836 836 c, t dbSNP:754718950
839 839 c, t dbSNP:778747927
846 846 a, t dbSNP:747954097
863 863 c, t dbSNP:376198310
864 864 a, g dbSNP:371047147
869 869 c, t dbSNP:61746135
870 870 a, g dbSNP:374430540
871 871 c, t dbSNP:775207650
887 887 a, c dbSNP:143502348
894 894 c, g dbSNP:143158098
900 900 g, t dbSNP:749487190
903 903 a, g dbSNP:768816689
909 909 a, g dbSNP:768259232
910 910 a, g dbSNP:773875619
911 911 c, g, t dbSNP:141361139
912 912 c, g, t dbSNP:150785634
914 914 a, g dbSNP:753449158
919 919 a, t dbSNP:754647855
925 925 g, t dbSNP:572691118
934 934 c, g dbSNP:139539716
939 939 c, t dbSNP:142730380
945 945 a, g dbSNP:777650849
956 956 a, g dbSNP:200230138
960 960 c, g dbSNP:767351911
964 964 c, t dbSNP:757230613
971 971 g, t dbSNP:146055714
982 982 a, g dbSNP:749434238
983 983 a, g dbSNP:140091480
989 989 c, t dbSNP:149797451
997 997 a, g dbSNP:748423582
999 999 c, t dbSNP:772283795
1000 1000 a, g dbSNP:145834905
1005 1005 a, g dbSNP:761242053
1006 1006 g, t dbSNP:771624598
1007 1007 a, g dbSNP:758722494
1010 1010 c, t dbSNP:199984362
1011 1011 a, g dbSNP:148549437
1013 1013 c, g dbSNP:752338067
1014 1014 a, g dbSNP:762630453
1017 1017 a, g dbSNP:200666877
1020 1020 c, t dbSNP:201017700
1024 1024 c, t dbSNP:190319017
1025 1025 a, g, t dbSNP:78850862
1026 1026 c, t dbSNP:755062941
1027 1027 a, g dbSNP:151108304
1030 1030 a, g dbSNP:141140102
1031 1031 c, t dbSNP:772288131
1038 1038 c, t dbSNP:778294490
1049 1049 a, g dbSNP:747308965
1051 1051 a, g dbSNP:771415744
1055 1055 c, t dbSNP:779856959
1058 1058 g, t dbSNP:200409709
1060 1060 c, g dbSNP:769223687
1069 1069 c, g dbSNP:775020136
1072 1072 a, g dbSNP:762530888
1083 1083 c, t dbSNP:763672752
1088 1088 a, g dbSNP:751211785
1095 1095 a, g dbSNP:367779834
1100 1100 g, t dbSNP:767279676
1101 1101 a, g dbSNP:750367920
1105 1105 a, g dbSNP:768419102
1119 1119 a, g dbSNP:778880264
1122 1122 g, t dbSNP:752763771
1126 1126 a, t dbSNP:199759051
1133 1133 c, t dbSNP:146939572
1134 1134 c, t dbSNP:541767318
1137 1137 c, t dbSNP:747218857
1138 1138 a, g dbSNP:757742093
1151 1151 c, g dbSNP:781659856
1155 1155 c, g dbSNP:746388689
1161 1161 a, g dbSNP:147562044
1167 1167 a, g dbSNP:142110631
1170 1170 c, g dbSNP:748897879
1173 1173 -, tg dbSNP:768537377
1180 1180 c, g dbSNP:768143061
1183 1183 c, t dbSNP:774040894
1189 1189 c, t dbSNP:761425574
1198 1198 c, t dbSNP:371304448
1203 1203 a, g dbSNP:773010399
1214 1214 c, g dbSNP:760542697
1226 1226 a, g dbSNP:766271224
1234 1234 g, t dbSNP:752857813
1261 1261 a, g dbSNP:147110981
1268 1268 -, atg dbSNP:759964480
1302 1302 -, agtc dbSNP:765798798
1355 1355 c, t dbSNP:372500407
1388 1388 c, t dbSNP:763457248
1411 1411 c, g dbSNP:191084574
1435 1435 a, g dbSNP:370556419
1443 1443 c, t dbSNP:778266553
1444 1444 a, g dbSNP:376774987
1454 1454 a, g dbSNP:527276181
1477 1477 a, g dbSNP:757443950
1507 1507 a, g dbSNP:767504019
1510 1510 a, c dbSNP:182183554
1530 1530 -, ata dbSNP:750367443
1535 1535 -, aac dbSNP:748069999
1583 1583 a, g dbSNP:751997214
1611 1611 c, g dbSNP:763916323
1624 1624 c, t dbSNP:186128975
1631 1631 c, t dbSNP:749191218
1633 1633 c, t dbSNP:549311870
1639 1639 a, g dbSNP:191003019
1643 1643 -, a dbSNP:778751966
1648 1648 a, t dbSNP:747765671
1679 1679 c, t dbSNP:757758095
1724 1724 g, t dbSNP:781596393

Target ORF information:

RefSeq Version NM_002362
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family A4 (MAGEA4), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_002362

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu02154D
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX479311.1 and AF274855.3. This sequence is a reference standard in the RefSeqGene project. Summary: This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (1) has the 5'-most exon compared to variants 2-4. All four variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX479311.1, DA912058.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001011548.1
CDS219..1172
Misc Feature(1)36..38(+)
Misc Feature(2)225..515(+)
Misc Feature(3)567..1079(+)
Exon (1)1..81
Gene:MAGEA4
Gene Synonym:
Exon (2)82..153
Gene:MAGEA4
Gene Synonym:
Exon (3)154..1724
Gene:MAGEA4
Gene Synonym:
Translation
Position Chain Variation Link
46 46 a, g dbSNP:771010980
85 85 a, c, t dbSNP:745816060
92 92 a, g dbSNP:774445671
97 97 a, g dbSNP:748368449
101 101 a, g dbSNP:368738694
102 102 c, t dbSNP:773613926
103 103 c, t dbSNP:763594807
104 104 a, g dbSNP:766855228
119 119 c, g dbSNP:777222146
128 128 a, g dbSNP:148460606
129 129 c, t dbSNP:764802119
143 143 g, t dbSNP:752191271
145 145 -, aga dbSNP:745310597
152 152 c, t dbSNP:758051896
155 155 c, t dbSNP:775056875
163 163 -, c dbSNP:769356179
164 164 c, t dbSNP:762298297
167 167 c, t dbSNP:183961725
168 168 a, g dbSNP:144129975
169 169 a, t dbSNP:761483501
176 176 a, g dbSNP:372125339
189 189 c, g dbSNP:767267374
190 190 c, t dbSNP:750267906
196 196 c, t dbSNP:756031183
201 201 a, g dbSNP:779923857
208 208 c, g dbSNP:1047246
216 216 a, g dbSNP:201648066
223 223 c, t dbSNP:777932203
229 229 a, g dbSNP:747163064
234 234 a, g dbSNP:771323726
235 235 a, g dbSNP:781676215
242 242 a, g dbSNP:746298946
246 246 c, t dbSNP:770283475
247 247 a, g dbSNP:775799958
248 248 c, t dbSNP:763535308
263 263 c, t dbSNP:200225562
264 264 a, g dbSNP:142773415
265 265 c, t dbSNP:780727589
273 273 c, g dbSNP:147367105
282 282 g, t dbSNP:767214466
283 283 c, t dbSNP:750215141
288 288 a, g dbSNP:760396181
292 292 c, t dbSNP:766211889
298 298 a, g, t dbSNP:753670573
299 299 c, t dbSNP:777687166
301 301 c, t dbSNP:772345662
302 302 a, g dbSNP:1047248
305 305 a, g dbSNP:150592561
307 307 c, t dbSNP:746953197
309 309 c, t dbSNP:61743686
315 315 a, t dbSNP:149323734
318 318 a, g dbSNP:749725686
331 331 c, t dbSNP:759301904
332 332 c, t dbSNP:369838724
337 337 a, t dbSNP:773721297
338 338 -, ctc dbSNP:769603439
338 338 c, t dbSNP:373247834
340 340 c, t dbSNP:41302158
346 346 c, g, t dbSNP:148498181
347 347 -, ctc dbSNP:372640194
347 347 c, g dbSNP:377034330
351 351 c, g dbSNP:201754404
354 354 c, t dbSNP:776532944
359 359 c, t dbSNP:759371757
365 365 c, t dbSNP:765279987
368 368 c, t dbSNP:752682980
373 373 a, t dbSNP:757344806
375 375 a, g dbSNP:767725718
377 377 a, c dbSNP:750630461
384 384 c, g dbSNP:370000858
391 391 a, g dbSNP:146569098
397 397 c, t dbSNP:780257307
421 421 a, g dbSNP:199930521
425 425 c, t dbSNP:758986419
432 432 a, t dbSNP:779325818
434 434 -, c dbSNP:34118273
434 434 a, c dbSNP:748667370
436 436 c, g, t dbSNP:763607469
437 437 c, g dbSNP:746613627
446 446 c, t dbSNP:770669132
449 449 c, t dbSNP:374428401
453 453 a, g dbSNP:759446643
454 454 c, g dbSNP:764951617
472 472 a, g dbSNP:377413340
473 473 a, t dbSNP:763013442
479 479 c, t dbSNP:767670794
483 483 a, g dbSNP:750579206
491 491 a, c dbSNP:756275786
497 497 g, t dbSNP:766690180
501 501 a, g dbSNP:754011082
504 504 c, t dbSNP:755313244
506 506 a, g dbSNP:201418399
510 510 a, g dbSNP:373757058
514 514 c, g, t dbSNP:200774631
515 515 a, g dbSNP:761277562
517 517 c, t dbSNP:140138379
522 522 a, g dbSNP:770490747
526 526 a, c dbSNP:776285619
533 533 c, g dbSNP:778217233
537 537 a, c dbSNP:373393448
538 538 a, g dbSNP:769670092
543 543 a, g dbSNP:775463116
554 554 c, g, t dbSNP:199755286
573 573 c, t dbSNP:773420574
578 578 c, t dbSNP:747399608
581 581 g, t dbSNP:766382651
585 585 c, t dbSNP:143837393
586 586 a, g dbSNP:759710688
594 594 a, c, g dbSNP:765557256
595 595 a, g dbSNP:151294389
605 605 a, g dbSNP:766749023
607 607 c, t dbSNP:752017073
613 613 c, g dbSNP:771228306
618 618 a, g dbSNP:181401508
635 635 a, c, t dbSNP:151060324
637 637 c, t dbSNP:745500251
641 641 c, g dbSNP:769617094
642 642 a, g dbSNP:779980180
643 643 a, g dbSNP:749161613
654 654 c, t dbSNP:768575994
655 655 a, g, t dbSNP:200822743
663 663 a, c dbSNP:771004302
665 665 c, t dbSNP:776616211
667 667 a, t dbSNP:759733983
674 674 c, t dbSNP:765504023
675 675 a, g dbSNP:200025917
679 679 a, g dbSNP:745602686
682 682 c, t dbSNP:763240762
686 686 c, t dbSNP:202047929
690 690 g, t dbSNP:752062577
691 691 c, g dbSNP:772388956
701 701 g, t dbSNP:757742313
716 716 c, t dbSNP:376596001
717 717 a, g, t dbSNP:778001194
727 727 c, t dbSNP:755823543
728 728 a, g dbSNP:779785942
734 734 c, t dbSNP:200853372
735 735 a, g dbSNP:1047251
738 738 a, g dbSNP:778605916
747 747 c, t dbSNP:748090014
751 751 c, t dbSNP:61743726
758 758 c, t dbSNP:776762269
760 760 c, t dbSNP:746017699
761 761 c, t dbSNP:781034122
764 764 c, t dbSNP:775637049
778 778 a, c, g, t dbSNP:199657597
785 785 c, t dbSNP:762366485
789 789 c, g dbSNP:141071979
791 791 a, g dbSNP:750943985
794 794 -, taa dbSNP:748977026
806 806 c, t dbSNP:755627386
818 818 a, g dbSNP:61741057
831 831 a, g dbSNP:753502144
833 833 c, t dbSNP:754718950
836 836 c, t dbSNP:778747927
843 843 a, t dbSNP:747954097
860 860 c, t dbSNP:376198310
861 861 a, g dbSNP:371047147
866 866 c, t dbSNP:61746135
867 867 a, g dbSNP:374430540
868 868 c, t dbSNP:775207650
884 884 a, c dbSNP:143502348
891 891 c, g dbSNP:143158098
897 897 g, t dbSNP:749487190
900 900 a, g dbSNP:768816689
906 906 a, g dbSNP:768259232
907 907 a, g dbSNP:773875619
908 908 c, g, t dbSNP:141361139
909 909 c, g, t dbSNP:150785634
911 911 a, g dbSNP:753449158
916 916 a, t dbSNP:754647855
922 922 g, t dbSNP:572691118
931 931 c, g dbSNP:139539716
936 936 c, t dbSNP:142730380
942 942 a, g dbSNP:777650849
953 953 a, g dbSNP:200230138
957 957 c, g dbSNP:767351911
961 961 c, t dbSNP:757230613
968 968 g, t dbSNP:146055714
979 979 a, g dbSNP:749434238
980 980 a, g dbSNP:140091480
986 986 c, t dbSNP:149797451
994 994 a, g dbSNP:748423582
996 996 c, t dbSNP:772283795
997 997 a, g dbSNP:145834905
1002 1002 a, g dbSNP:761242053
1003 1003 g, t dbSNP:771624598
1004 1004 a, g dbSNP:758722494
1007 1007 c, t dbSNP:199984362
1008 1008 a, g dbSNP:148549437
1010 1010 c, g dbSNP:752338067
1011 1011 a, g dbSNP:762630453
1014 1014 a, g dbSNP:200666877
1017 1017 c, t dbSNP:201017700
1021 1021 c, t dbSNP:190319017
1022 1022 a, g, t dbSNP:78850862
1023 1023 c, t dbSNP:755062941
1024 1024 a, g dbSNP:151108304
1027 1027 a, g dbSNP:141140102
1028 1028 c, t dbSNP:772288131
1035 1035 c, t dbSNP:778294490
1046 1046 a, g dbSNP:747308965
1048 1048 a, g dbSNP:771415744
1052 1052 c, t dbSNP:779856959
1055 1055 g, t dbSNP:200409709
1057 1057 c, g dbSNP:769223687
1066 1066 c, g dbSNP:775020136
1069 1069 a, g dbSNP:762530888
1080 1080 c, t dbSNP:763672752
1085 1085 a, g dbSNP:751211785
1092 1092 a, g dbSNP:367779834
1097 1097 g, t dbSNP:767279676
1098 1098 a, g dbSNP:750367920
1102 1102 a, g dbSNP:768419102
1116 1116 a, g dbSNP:778880264
1119 1119 g, t dbSNP:752763771
1123 1123 a, t dbSNP:199759051
1130 1130 c, t dbSNP:146939572
1131 1131 c, t dbSNP:541767318
1134 1134 c, t dbSNP:747218857
1135 1135 a, g dbSNP:757742093
1148 1148 c, g dbSNP:781659856
1152 1152 c, g dbSNP:746388689
1158 1158 a, g dbSNP:147562044
1164 1164 a, g dbSNP:142110631
1167 1167 c, g dbSNP:748897879
1170 1170 -, tg dbSNP:768537377
1177 1177 c, g dbSNP:768143061
1180 1180 c, t dbSNP:774040894
1186 1186 c, t dbSNP:761425574
1195 1195 c, t dbSNP:371304448
1200 1200 a, g dbSNP:773010399
1211 1211 c, g dbSNP:760542697
1223 1223 a, g dbSNP:766271224
1231 1231 g, t dbSNP:752857813
1258 1258 a, g dbSNP:147110981
1265 1265 -, atg dbSNP:759964480
1299 1299 -, agtc dbSNP:765798798
1352 1352 c, t dbSNP:372500407
1385 1385 c, t dbSNP:763457248
1408 1408 c, g dbSNP:191084574
1432 1432 a, g dbSNP:370556419
1440 1440 c, t dbSNP:778266553
1441 1441 a, g dbSNP:376774987
1451 1451 a, g dbSNP:527276181
1474 1474 a, g dbSNP:757443950
1504 1504 a, g dbSNP:767504019
1507 1507 a, c dbSNP:182183554
1527 1527 -, ata dbSNP:750367443
1532 1532 -, aac dbSNP:748069999
1580 1580 a, g dbSNP:751997214
1608 1608 c, g dbSNP:763916323
1621 1621 c, t dbSNP:186128975
1628 1628 c, t dbSNP:749191218
1630 1630 c, t dbSNP:549311870
1636 1636 a, g dbSNP:191003019
1640 1640 -, a dbSNP:778751966
1645 1645 a, t dbSNP:747765671
1676 1676 c, t dbSNP:757758095
1721 1721 g, t dbSNP:781596393

Target ORF information:

RefSeq Version NM_001011548
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family A4 (MAGEA4), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001011548

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu02154D
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM548926.1 and AF274855.3. Summary: This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All four variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM548926.1, BX361006.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001011549.1
CDS216..1169
Misc Feature(1)222..512(+)
Misc Feature(2)564..1076(+)
Exon (1)1..78
Gene:MAGEA4
Gene Synonym:
Exon (2)79..150
Gene:MAGEA4
Gene Synonym:
Exon (3)151..1721
Gene:MAGEA4
Gene Synonym:
Translation
Position Chain Variation Link
82 82 a, c, t dbSNP:745816060
89 89 a, g dbSNP:774445671
94 94 a, g dbSNP:748368449
98 98 a, g dbSNP:368738694
99 99 c, t dbSNP:773613926
100 100 c, t dbSNP:763594807
101 101 a, g dbSNP:766855228
116 116 c, g dbSNP:777222146
125 125 a, g dbSNP:148460606
126 126 c, t dbSNP:764802119
140 140 g, t dbSNP:752191271
142 142 -, aga dbSNP:745310597
149 149 c, t dbSNP:758051896
152 152 c, t dbSNP:775056875
160 160 -, c dbSNP:769356179
161 161 c, t dbSNP:762298297
164 164 c, t dbSNP:183961725
165 165 a, g dbSNP:144129975
166 166 a, t dbSNP:761483501
173 173 a, g dbSNP:372125339
186 186 c, g dbSNP:767267374
187 187 c, t dbSNP:750267906
193 193 c, t dbSNP:756031183
198 198 a, g dbSNP:779923857
205 205 c, g dbSNP:1047246
213 213 a, g dbSNP:201648066
220 220 c, t dbSNP:777932203
226 226 a, g dbSNP:747163064
231 231 a, g dbSNP:771323726
232 232 a, g dbSNP:781676215
239 239 a, g dbSNP:746298946
243 243 c, t dbSNP:770283475
244 244 a, g dbSNP:775799958
245 245 c, t dbSNP:763535308
260 260 c, t dbSNP:200225562
261 261 a, g dbSNP:142773415
262 262 c, t dbSNP:780727589
270 270 c, g dbSNP:147367105
279 279 g, t dbSNP:767214466
280 280 c, t dbSNP:750215141
285 285 a, g dbSNP:760396181
289 289 c, t dbSNP:766211889
295 295 a, g, t dbSNP:753670573
296 296 c, t dbSNP:777687166
298 298 c, t dbSNP:772345662
299 299 a, g dbSNP:1047248
302 302 a, g dbSNP:150592561
304 304 c, t dbSNP:746953197
306 306 c, t dbSNP:61743686
312 312 a, t dbSNP:149323734
315 315 a, g dbSNP:749725686
328 328 c, t dbSNP:759301904
329 329 c, t dbSNP:369838724
334 334 a, t dbSNP:773721297
335 335 -, ctc dbSNP:769603439
335 335 c, t dbSNP:373247834
337 337 c, t dbSNP:41302158
343 343 c, g, t dbSNP:148498181
344 344 -, ctc dbSNP:372640194
344 344 c, g dbSNP:377034330
348 348 c, g dbSNP:201754404
351 351 c, t dbSNP:776532944
356 356 c, t dbSNP:759371757
362 362 c, t dbSNP:765279987
365 365 c, t dbSNP:752682980
370 370 a, t dbSNP:757344806
372 372 a, g dbSNP:767725718
374 374 a, c dbSNP:750630461
381 381 c, g dbSNP:370000858
388 388 a, g dbSNP:146569098
394 394 c, t dbSNP:780257307
418 418 a, g dbSNP:199930521
422 422 c, t dbSNP:758986419
429 429 a, t dbSNP:779325818
431 431 -, c dbSNP:34118273
431 431 a, c dbSNP:748667370
433 433 c, g, t dbSNP:763607469
434 434 c, g dbSNP:746613627
443 443 c, t dbSNP:770669132
446 446 c, t dbSNP:374428401
450 450 a, g dbSNP:759446643
451 451 c, g dbSNP:764951617
469 469 a, g dbSNP:377413340
470 470 a, t dbSNP:763013442
476 476 c, t dbSNP:767670794
480 480 a, g dbSNP:750579206
488 488 a, c dbSNP:756275786
494 494 g, t dbSNP:766690180
498 498 a, g dbSNP:754011082
501 501 c, t dbSNP:755313244
503 503 a, g dbSNP:201418399
507 507 a, g dbSNP:373757058
511 511 c, g, t dbSNP:200774631
512 512 a, g dbSNP:761277562
514 514 c, t dbSNP:140138379
519 519 a, g dbSNP:770490747
523 523 a, c dbSNP:776285619
530 530 c, g dbSNP:778217233
534 534 a, c dbSNP:373393448
535 535 a, g dbSNP:769670092
540 540 a, g dbSNP:775463116
551 551 c, g, t dbSNP:199755286
570 570 c, t dbSNP:773420574
575 575 c, t dbSNP:747399608
578 578 g, t dbSNP:766382651
582 582 c, t dbSNP:143837393
583 583 a, g dbSNP:759710688
591 591 a, c, g dbSNP:765557256
592 592 a, g dbSNP:151294389
602 602 a, g dbSNP:766749023
604 604 c, t dbSNP:752017073
610 610 c, g dbSNP:771228306
615 615 a, g dbSNP:181401508
632 632 a, c, t dbSNP:151060324
634 634 c, t dbSNP:745500251
638 638 c, g dbSNP:769617094
639 639 a, g dbSNP:779980180
640 640 a, g dbSNP:749161613
651 651 c, t dbSNP:768575994
652 652 a, g, t dbSNP:200822743
660 660 a, c dbSNP:771004302
662 662 c, t dbSNP:776616211
664 664 a, t dbSNP:759733983
671 671 c, t dbSNP:765504023
672 672 a, g dbSNP:200025917
676 676 a, g dbSNP:745602686
679 679 c, t dbSNP:763240762
683 683 c, t dbSNP:202047929
687 687 g, t dbSNP:752062577
688 688 c, g dbSNP:772388956
698 698 g, t dbSNP:757742313
713 713 c, t dbSNP:376596001
714 714 a, g, t dbSNP:778001194
724 724 c, t dbSNP:755823543
725 725 a, g dbSNP:779785942
731 731 c, t dbSNP:200853372
732 732 a, g dbSNP:1047251
735 735 a, g dbSNP:778605916
744 744 c, t dbSNP:748090014
748 748 c, t dbSNP:61743726
755 755 c, t dbSNP:776762269
757 757 c, t dbSNP:746017699
758 758 c, t dbSNP:781034122
761 761 c, t dbSNP:775637049
775 775 a, c, g, t dbSNP:199657597
782 782 c, t dbSNP:762366485
786 786 c, g dbSNP:141071979
788 788 a, g dbSNP:750943985
791 791 -, taa dbSNP:748977026
803 803 c, t dbSNP:755627386
815 815 a, g dbSNP:61741057
828 828 a, g dbSNP:753502144
830 830 c, t dbSNP:754718950
833 833 c, t dbSNP:778747927
840 840 a, t dbSNP:747954097
857 857 c, t dbSNP:376198310
858 858 a, g dbSNP:371047147
863 863 c, t dbSNP:61746135
864 864 a, g dbSNP:374430540
865 865 c, t dbSNP:775207650
881 881 a, c dbSNP:143502348
888 888 c, g dbSNP:143158098
894 894 g, t dbSNP:749487190
897 897 a, g dbSNP:768816689
903 903 a, g dbSNP:768259232
904 904 a, g dbSNP:773875619
905 905 c, g, t dbSNP:141361139
906 906 c, g, t dbSNP:150785634
908 908 a, g dbSNP:753449158
913 913 a, t dbSNP:754647855
919 919 g, t dbSNP:572691118
928 928 c, g dbSNP:139539716
933 933 c, t dbSNP:142730380
939 939 a, g dbSNP:777650849
950 950 a, g dbSNP:200230138
954 954 c, g dbSNP:767351911
958 958 c, t dbSNP:757230613
965 965 g, t dbSNP:146055714
976 976 a, g dbSNP:749434238
977 977 a, g dbSNP:140091480
983 983 c, t dbSNP:149797451
991 991 a, g dbSNP:748423582
993 993 c, t dbSNP:772283795
994 994 a, g dbSNP:145834905
999 999 a, g dbSNP:761242053
1000 1000 g, t dbSNP:771624598
1001 1001 a, g dbSNP:758722494
1004 1004 c, t dbSNP:199984362
1005 1005 a, g dbSNP:148549437
1007 1007 c, g dbSNP:752338067
1008 1008 a, g dbSNP:762630453
1011 1011 a, g dbSNP:200666877
1014 1014 c, t dbSNP:201017700
1018 1018 c, t dbSNP:190319017
1019 1019 a, g, t dbSNP:78850862
1020 1020 c, t dbSNP:755062941
1021 1021 a, g dbSNP:151108304
1024 1024 a, g dbSNP:141140102
1025 1025 c, t dbSNP:772288131
1032 1032 c, t dbSNP:778294490
1043 1043 a, g dbSNP:747308965
1045 1045 a, g dbSNP:771415744
1049 1049 c, t dbSNP:779856959
1052 1052 g, t dbSNP:200409709
1054 1054 c, g dbSNP:769223687
1063 1063 c, g dbSNP:775020136
1066 1066 a, g dbSNP:762530888
1077 1077 c, t dbSNP:763672752
1082 1082 a, g dbSNP:751211785
1089 1089 a, g dbSNP:367779834
1094 1094 g, t dbSNP:767279676
1095 1095 a, g dbSNP:750367920
1099 1099 a, g dbSNP:768419102
1113 1113 a, g dbSNP:778880264
1116 1116 g, t dbSNP:752763771
1120 1120 a, t dbSNP:199759051
1127 1127 c, t dbSNP:146939572
1128 1128 c, t dbSNP:541767318
1131 1131 c, t dbSNP:747218857
1132 1132 a, g dbSNP:757742093
1145 1145 c, g dbSNP:781659856
1149 1149 c, g dbSNP:746388689
1155 1155 a, g dbSNP:147562044
1161 1161 a, g dbSNP:142110631
1164 1164 c, g dbSNP:748897879
1167 1167 -, tg dbSNP:768537377
1174 1174 c, g dbSNP:768143061
1177 1177 c, t dbSNP:774040894
1183 1183 c, t dbSNP:761425574
1192 1192 c, t dbSNP:371304448
1197 1197 a, g dbSNP:773010399
1208 1208 c, g dbSNP:760542697
1220 1220 a, g dbSNP:766271224
1228 1228 g, t dbSNP:752857813
1255 1255 a, g dbSNP:147110981
1262 1262 -, atg dbSNP:759964480
1296 1296 -, agtc dbSNP:765798798
1349 1349 c, t dbSNP:372500407
1382 1382 c, t dbSNP:763457248
1405 1405 c, g dbSNP:191084574
1429 1429 a, g dbSNP:370556419
1437 1437 c, t dbSNP:778266553
1438 1438 a, g dbSNP:376774987
1448 1448 a, g dbSNP:527276181
1471 1471 a, g dbSNP:757443950
1501 1501 a, g dbSNP:767504019
1504 1504 a, c dbSNP:182183554
1524 1524 -, ata dbSNP:750367443
1529 1529 -, aac dbSNP:748069999
1577 1577 a, g dbSNP:751997214
1605 1605 c, g dbSNP:763916323
1618 1618 c, t dbSNP:186128975
1625 1625 c, t dbSNP:749191218
1627 1627 c, t dbSNP:549311870
1633 1633 a, g dbSNP:191003019
1637 1637 -, a dbSNP:778751966
1642 1642 a, t dbSNP:747765671
1673 1673 c, t dbSNP:757758095
1718 1718 g, t dbSNP:781596393

Target ORF information:

RefSeq Version NM_001011549
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family A4 (MAGEA4), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001011549

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu02154D
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB120212.1 and AF274855.3. Summary: This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. All four variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC017723.1, AK313862.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001011550.1
CDS207..1160
Misc Feature(1)213..503(+)
Misc Feature(2)555..1067(+)
Exon (1)1..69
Gene:MAGEA4
Gene Synonym:
Exon (2)70..141
Gene:MAGEA4
Gene Synonym:
Exon (3)142..1712
Gene:MAGEA4
Gene Synonym:
Translation
Position Chain Variation Link
73 73 a, c, t dbSNP:745816060
80 80 a, g dbSNP:774445671
85 85 a, g dbSNP:748368449
89 89 a, g dbSNP:368738694
90 90 c, t dbSNP:773613926
91 91 c, t dbSNP:763594807
92 92 a, g dbSNP:766855228
107 107 c, g dbSNP:777222146
116 116 a, g dbSNP:148460606
117 117 c, t dbSNP:764802119
131 131 g, t dbSNP:752191271
133 133 -, aga dbSNP:745310597
140 140 c, t dbSNP:758051896
143 143 c, t dbSNP:775056875
151 151 -, c dbSNP:769356179
152 152 c, t dbSNP:762298297
155 155 c, t dbSNP:183961725
156 156 a, g dbSNP:144129975
157 157 a, t dbSNP:761483501
164 164 a, g dbSNP:372125339
177 177 c, g dbSNP:767267374
178 178 c, t dbSNP:750267906
184 184 c, t dbSNP:756031183
189 189 a, g dbSNP:779923857
196 196 c, g dbSNP:1047246
204 204 a, g dbSNP:201648066
211 211 c, t dbSNP:777932203
217 217 a, g dbSNP:747163064
222 222 a, g dbSNP:771323726
223 223 a, g dbSNP:781676215
230 230 a, g dbSNP:746298946
234 234 c, t dbSNP:770283475
235 235 a, g dbSNP:775799958
236 236 c, t dbSNP:763535308
251 251 c, t dbSNP:200225562
252 252 a, g dbSNP:142773415
253 253 c, t dbSNP:780727589
261 261 c, g dbSNP:147367105
270 270 g, t dbSNP:767214466
271 271 c, t dbSNP:750215141
276 276 a, g dbSNP:760396181
280 280 c, t dbSNP:766211889
286 286 a, g, t dbSNP:753670573
287 287 c, t dbSNP:777687166
289 289 c, t dbSNP:772345662
290 290 a, g dbSNP:1047248
293 293 a, g dbSNP:150592561
295 295 c, t dbSNP:746953197
297 297 c, t dbSNP:61743686
303 303 a, t dbSNP:149323734
306 306 a, g dbSNP:749725686
319 319 c, t dbSNP:759301904
320 320 c, t dbSNP:369838724
325 325 a, t dbSNP:773721297
326 326 -, ctc dbSNP:769603439
326 326 c, t dbSNP:373247834
328 328 c, t dbSNP:41302158
334 334 c, g, t dbSNP:148498181
335 335 -, ctc dbSNP:372640194
335 335 c, g dbSNP:377034330
339 339 c, g dbSNP:201754404
342 342 c, t dbSNP:776532944
347 347 c, t dbSNP:759371757
353 353 c, t dbSNP:765279987
356 356 c, t dbSNP:752682980
361 361 a, t dbSNP:757344806
363 363 a, g dbSNP:767725718
365 365 a, c dbSNP:750630461
372 372 c, g dbSNP:370000858
379 379 a, g dbSNP:146569098
385 385 c, t dbSNP:780257307
409 409 a, g dbSNP:199930521
413 413 c, t dbSNP:758986419
420 420 a, t dbSNP:779325818
422 422 -, c dbSNP:34118273
422 422 a, c dbSNP:748667370
424 424 c, g, t dbSNP:763607469
425 425 c, g dbSNP:746613627
434 434 c, t dbSNP:770669132
437 437 c, t dbSNP:374428401
441 441 a, g dbSNP:759446643
442 442 c, g dbSNP:764951617
460 460 a, g dbSNP:377413340
461 461 a, t dbSNP:763013442
467 467 c, t dbSNP:767670794
471 471 a, g dbSNP:750579206
479 479 a, c dbSNP:756275786
485 485 g, t dbSNP:766690180
489 489 a, g dbSNP:754011082
492 492 c, t dbSNP:755313244
494 494 a, g dbSNP:201418399
498 498 a, g dbSNP:373757058
502 502 c, g, t dbSNP:200774631
503 503 a, g dbSNP:761277562
505 505 c, t dbSNP:140138379
510 510 a, g dbSNP:770490747
514 514 a, c dbSNP:776285619
521 521 c, g dbSNP:778217233
525 525 a, c dbSNP:373393448
526 526 a, g dbSNP:769670092
531 531 a, g dbSNP:775463116
542 542 c, g, t dbSNP:199755286
561 561 c, t dbSNP:773420574
566 566 c, t dbSNP:747399608
569 569 g, t dbSNP:766382651
573 573 c, t dbSNP:143837393
574 574 a, g dbSNP:759710688
582 582 a, c, g dbSNP:765557256
583 583 a, g dbSNP:151294389
593 593 a, g dbSNP:766749023
595 595 c, t dbSNP:752017073
601 601 c, g dbSNP:771228306
606 606 a, g dbSNP:181401508
623 623 a, c, t dbSNP:151060324
625 625 c, t dbSNP:745500251
629 629 c, g dbSNP:769617094
630 630 a, g dbSNP:779980180
631 631 a, g dbSNP:749161613
642 642 c, t dbSNP:768575994
643 643 a, g, t dbSNP:200822743
651 651 a, c dbSNP:771004302
653 653 c, t dbSNP:776616211
655 655 a, t dbSNP:759733983
662 662 c, t dbSNP:765504023
663 663 a, g dbSNP:200025917
667 667 a, g dbSNP:745602686
670 670 c, t dbSNP:763240762
674 674 c, t dbSNP:202047929
678 678 g, t dbSNP:752062577
679 679 c, g dbSNP:772388956
689 689 g, t dbSNP:757742313
704 704 c, t dbSNP:376596001
705 705 a, g, t dbSNP:778001194
715 715 c, t dbSNP:755823543
716 716 a, g dbSNP:779785942
722 722 c, t dbSNP:200853372
723 723 a, g dbSNP:1047251
726 726 a, g dbSNP:778605916
735 735 c, t dbSNP:748090014
739 739 c, t dbSNP:61743726
746 746 c, t dbSNP:776762269
748 748 c, t dbSNP:746017699
749 749 c, t dbSNP:781034122
752 752 c, t dbSNP:775637049
766 766 a, c, g, t dbSNP:199657597
773 773 c, t dbSNP:762366485
777 777 c, g dbSNP:141071979
779 779 a, g dbSNP:750943985
782 782 -, taa dbSNP:748977026
794 794 c, t dbSNP:755627386
806 806 a, g dbSNP:61741057
819 819 a, g dbSNP:753502144
821 821 c, t dbSNP:754718950
824 824 c, t dbSNP:778747927
831 831 a, t dbSNP:747954097
848 848 c, t dbSNP:376198310
849 849 a, g dbSNP:371047147
854 854 c, t dbSNP:61746135
855 855 a, g dbSNP:374430540
856 856 c, t dbSNP:775207650
872 872 a, c dbSNP:143502348
879 879 c, g dbSNP:143158098
885 885 g, t dbSNP:749487190
888 888 a, g dbSNP:768816689
894 894 a, g dbSNP:768259232
895 895 a, g dbSNP:773875619
896 896 c, g, t dbSNP:141361139
897 897 c, g, t dbSNP:150785634
899 899 a, g dbSNP:753449158
904 904 a, t dbSNP:754647855
910 910 g, t dbSNP:572691118
919 919 c, g dbSNP:139539716
924 924 c, t dbSNP:142730380
930 930 a, g dbSNP:777650849
941 941 a, g dbSNP:200230138
945 945 c, g dbSNP:767351911
949 949 c, t dbSNP:757230613
956 956 g, t dbSNP:146055714
967 967 a, g dbSNP:749434238
968 968 a, g dbSNP:140091480
974 974 c, t dbSNP:149797451
982 982 a, g dbSNP:748423582
984 984 c, t dbSNP:772283795
985 985 a, g dbSNP:145834905
990 990 a, g dbSNP:761242053
991 991 g, t dbSNP:771624598
992 992 a, g dbSNP:758722494
995 995 c, t dbSNP:199984362
996 996 a, g dbSNP:148549437
998 998 c, g dbSNP:752338067
999 999 a, g dbSNP:762630453
1002 1002 a, g dbSNP:200666877
1005 1005 c, t dbSNP:201017700
1009 1009 c, t dbSNP:190319017
1010 1010 a, g, t dbSNP:78850862
1011 1011 c, t dbSNP:755062941
1012 1012 a, g dbSNP:151108304
1015 1015 a, g dbSNP:141140102
1016 1016 c, t dbSNP:772288131
1023 1023 c, t dbSNP:778294490
1034 1034 a, g dbSNP:747308965
1036 1036 a, g dbSNP:771415744
1040 1040 c, t dbSNP:779856959
1043 1043 g, t dbSNP:200409709
1045 1045 c, g dbSNP:769223687
1054 1054 c, g dbSNP:775020136
1057 1057 a, g dbSNP:762530888
1068 1068 c, t dbSNP:763672752
1073 1073 a, g dbSNP:751211785
1080 1080 a, g dbSNP:367779834
1085 1085 g, t dbSNP:767279676
1086 1086 a, g dbSNP:750367920
1090 1090 a, g dbSNP:768419102
1104 1104 a, g dbSNP:778880264
1107 1107 g, t dbSNP:752763771
1111 1111 a, t dbSNP:199759051
1118 1118 c, t dbSNP:146939572
1119 1119 c, t dbSNP:541767318
1122 1122 c, t dbSNP:747218857
1123 1123 a, g dbSNP:757742093
1136 1136 c, g dbSNP:781659856
1140 1140 c, g dbSNP:746388689
1146 1146 a, g dbSNP:147562044
1152 1152 a, g dbSNP:142110631
1155 1155 c, g dbSNP:748897879
1158 1158 -, tg dbSNP:768537377
1165 1165 c, g dbSNP:768143061
1168 1168 c, t dbSNP:774040894
1174 1174 c, t dbSNP:761425574
1183 1183 c, t dbSNP:371304448
1188 1188 a, g dbSNP:773010399
1199 1199 c, g dbSNP:760542697
1211 1211 a, g dbSNP:766271224
1219 1219 g, t dbSNP:752857813
1246 1246 a, g dbSNP:147110981
1253 1253 -, atg dbSNP:759964480
1287 1287 -, agtc dbSNP:765798798
1340 1340 c, t dbSNP:372500407
1373 1373 c, t dbSNP:763457248
1396 1396 c, g dbSNP:191084574
1420 1420 a, g dbSNP:370556419
1428 1428 c, t dbSNP:778266553
1429 1429 a, g dbSNP:376774987
1439 1439 a, g dbSNP:527276181
1462 1462 a, g dbSNP:757443950
1492 1492 a, g dbSNP:767504019
1495 1495 a, c dbSNP:182183554
1515 1515 -, ata dbSNP:750367443
1520 1520 -, aac dbSNP:748069999
1568 1568 a, g dbSNP:751997214
1596 1596 c, g dbSNP:763916323
1609 1609 c, t dbSNP:186128975
1616 1616 c, t dbSNP:749191218
1618 1618 c, t dbSNP:549311870
1624 1624 a, g dbSNP:191003019
1628 1628 -, a dbSNP:778751966
1633 1633 a, t dbSNP:747765671
1664 1664 c, t dbSNP:757758095
1709 1709 g, t dbSNP:781596393

Target ORF information:

RefSeq Version NM_001011550
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family A4 (MAGEA4), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001011550

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.