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MAGEA5 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MAGEA5
Entrez Gene ID 4104
Full Name melanoma antigen family A5
Synonyms CT1.5, MAGE5, MAGEA4
General protein information
Preferred Names
melanoma-associated antigen 5
Names
melanoma-associated antigen 5
MAGE-5 antigen
MAGE-5a antigen
MAGE-5b antigen
cancer/testis antigen 1.5
cancer/testis antigen family 1, member 5
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xq28

Summary This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This MAGEA gene encodes a protein that is C-terminally truncated compared to other family members, and this gene can be alternatively interpreted to be a pseudogene. The protein is represented in this Gene record in accordance with the assumed protein-coding status defined in the literature. Read-through transcription exists between this gene and the upstream melanoma antigen family A, 10 (MAGEA10) gene. [provided by RefSeq, Oct 2011]. lac of sum
Disorder MIM:

300340

Disorder Html:





The following MAGEA5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGEA5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu08405 NM_021049 Homo sapiens melanoma antigen family A5 (MAGEA5), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu08405
Accession Version NM_021049.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 375bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC109187.1, AF274856.3 and AI200443.1. This sequence is a reference standard in the RefSeqGene project. On Oct 18, 2011 this sequence version replaced gi:58530874. Summary: This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This MAGEA gene encodes a protein that is C-terminally truncated compared to other family members, and this gene can be alternatively interpreted to be a pseudogene. The protein is represented in this Gene record in accordance with the assumed protein-coding status defined in the literature. Read-through transcription exists between this gene and the upstream melanoma antigen family A, 10 (MAGEA10) gene. [provided by RefSeq, Oct 2011]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC109187.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2148874, SAMEA2149398 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
ATGTCTCTTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA CACCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGTGCAGGCT GCCACTACTG AGGAGCAGGA GGCTGTGTCC
TCCTCCTCTC CTCTGGTCCC AGGCACCCTG GGGGAGGTGC CTGCTGCTGG GTCACCAGGT
CCTCTCAAGA GTCCTCAGGG AGCCTCCGCC ATCCCCACTG CCATCGATTT CACTCTATGG
AGGCAATCCA TTAAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTCCCCTGAC
CCAGAGTCTG TGTTCCGAGC AGCACTCAGT AAGAAGGTGG CTGACTTGAT TCATTTTCTG
CTCCTCAAGT ATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_066387.1
CDS173..547
Misc Feature(1)38..40(+)
Misc Feature(2)179..466(+)
Exon (1)1..20
Gene:MAGEA5
Gene Synonym:
Exon (2)21..107
Gene:MAGEA5
Gene Synonym:
Exon (3)108..1664
Gene:MAGEA5
Gene Synonym:
Translation
Position Chain Variation Link
1 1 a, g dbSNP:746446179
34 34 a, t dbSNP:751572845
60 60 c, g dbSNP:745933390
66 66 g, t dbSNP:192002723
127 127 c, t dbSNP:759374220
135 135 c, t dbSNP:776249481
139 139 c, t dbSNP:770635382
142 142 -, ac dbSNP:747884665
152 152 a, g dbSNP:758558219
156 156 c, t dbSNP:186697412
157 157 a, g dbSNP:771638214
163 163 c, t dbSNP:748567605
167 167 c, g dbSNP:779607707
169 169 -, c dbSNP:774283076
169 169 c, t dbSNP:755375999
170 170 a, g dbSNP:757429005
171 171 c, t dbSNP:754312403
172 172 c, t dbSNP:181431328
173 173 a, g dbSNP:190192290
174 174 c, t dbSNP:376205956
184 184 a, g dbSNP:140384326
193 193 g, t dbSNP:766823244
195 195 a, g dbSNP:200126235
197 197 a, c dbSNP:765213425
216 216 c, g dbSNP:759318732
218 218 c, t dbSNP:370449552
243 243 g, t dbSNP:138740123
250 250 a, g dbSNP:770723396
252 252 c, g dbSNP:760199456
259 259 a, c, g dbSNP:188387
260 260 c, g dbSNP:771455840
265 265 c, t dbSNP:747746437
273 273 a, c dbSNP:779550506
280 280 c, g dbSNP:591428
281 281 a, g dbSNP:769353200
287 287 a, g dbSNP:749819073
292 292 c, t dbSNP:138018950
298 298 -, ctctcctc dbSNP:768478690
298 298 c, t dbSNP:756531076
307 307 a, g dbSNP:750577858
314 314 c, g dbSNP:591483
329 329 a, g dbSNP:781524716
330 330 -, aggt dbSNP:748925654
332 332 c, t dbSNP:757270652
336 336 c, t dbSNP:763552610
337 337 c, t dbSNP:764159649
350 350 c, g dbSNP:591530
351 351 a, g dbSNP:201646238
354 354 c, t dbSNP:267606383
362 362 a, g dbSNP:377236337
364 364 g, t dbSNP:766125848
367 367 g, t dbSNP:760560384
368 368 c, t dbSNP:772721895
378 378 c, t dbSNP:767262270
379 379 a, c, t dbSNP:140955654
397 397 c, t dbSNP:778167841
405 405 c, t dbSNP:749771814
406 406 c, t dbSNP:776178518
408 408 c, t dbSNP:770032602
411 411 a, g dbSNP:146488250
417 417 a, c dbSNP:754768194
420 420 a, c dbSNP:144035929
430 430 c, t dbSNP:781263294
432 432 c, g dbSNP:757429818
439 439 c, t dbSNP:747243846
446 446 a, g dbSNP:777778767
452 452 c, g dbSNP:189139222
457 457 a, c, g dbSNP:769408504
459 459 a, g dbSNP:766391930
473 473 c, t dbSNP:755851797
476 476 c, g, t dbSNP:592351
480 480 c, t dbSNP:767220280
482 482 c, g dbSNP:761473737
488 488 c, t dbSNP:774170350
489 489 a, g dbSNP:763665105
492 492 a, c dbSNP:762598769
497 497 c, t dbSNP:776127310
508 508 a, g dbSNP:592392
524 524 c, t dbSNP:267606382
529 529 c, t dbSNP:148863075
537 537 c, t dbSNP:746362148
545 545 c, t dbSNP:776992069
546 546 a, g dbSNP:771220505
547 547 a, g, t dbSNP:753712471
549 549 c, t dbSNP:778012861
550 550 c, g dbSNP:758453289
558 558 c, t dbSNP:2020030
559 559 a, g dbSNP:760730793
560 560 c, g dbSNP:756013431
572 572 c, g dbSNP:149269310
575 575 a, g dbSNP:780993348
583 583 g, t dbSNP:757157297
585 585 ac, ga dbSNP:386828484
585 585 a, c, g dbSNP:182002707
586 586 a, c, t dbSNP:210576
587 587 a, g dbSNP:764662202
595 595 a, g dbSNP:760036900
616 616 g, t dbSNP:549709414
618 618 a, t dbSNP:210575
625 625 c, t dbSNP:773945105
647 647 ca, gc dbSNP:34155747
678 678 c, t dbSNP:111586310
679 679 a, g dbSNP:190372555
683 683 a, c dbSNP:755237126
730 730 a, g dbSNP:184593633
738 738 c, g dbSNP:3564
740 740 -, ggttgataa dbSNP:755054571
744 744 -, gataa dbSNP:67540048
744 744 g, t dbSNP:113411567
746 746 g, t dbSNP:371159114
747 747 a, t dbSNP:377429389
748 748 -, gataa dbSNP:767098917
748 748 a, t dbSNP:372610677
769 769 c, t dbSNP:750844307
770 770 a, g dbSNP:138458678
818 818 c, t dbSNP:763787183
841 841 a, g dbSNP:763198016
848 848 g, t dbSNP:762350648
849 849 a, g dbSNP:3565
862 862 c, t dbSNP:764789316
890 890 a, t dbSNP:192030624
908 908 a, g dbSNP:377615963
949 949 a, g dbSNP:770593375
955 955 g, t dbSNP:377302305
977 977 a, c dbSNP:640032
1005 1005 c, t dbSNP:187178184
1017 1017 a, c, g dbSNP:140688744
1072 1072 c, g dbSNP:746332509
1073 1073 c, g dbSNP:640475
1077 1077 a, g dbSNP:210574
1149 1149 c, g dbSNP:2471383
1165 1165 c, t dbSNP:755086401
1173 1173 c, t dbSNP:112056043
1181 1181 c, g dbSNP:210573
1257 1257 c, g dbSNP:192792759
1269 1269 c, t dbSNP:376679899
1298 1298 c, t dbSNP:750772453
1308 1308 a, c dbSNP:758732709
1323 1323 c, t dbSNP:210572
1328 1328 c, t dbSNP:779956292
1340 1340 g, t dbSNP:757515075
1356 1356 c, t dbSNP:757751927
1360 1360 a, g dbSNP:369034674
1383 1383 c, g dbSNP:750334771
1431 1431 a, c dbSNP:764662292
1528 1528 c, g dbSNP:2452124
1531 1531 a, t dbSNP:753384927
1544 1544 a, g dbSNP:187534310
1575 1575 c, t dbSNP:210571
1584 1584 a, t dbSNP:773127690
1593 1593 a, t dbSNP:2471978
1598 1598 -, a dbSNP:761771479

Target ORF information:

RefSeq Version NM_021049
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family A5 (MAGEA5), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_021049

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGTCTCTTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA CACCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGTGCAGGCT GCCACTACTG AGGAGCAGGA GGCTGTGTCC
TCCTCCTCTC CTCTGGTCCC AGGCACCCTG GGGGAGGTGC CTGCTGCTGG GTCACCAGGT
CCTCTCAAGA GTCCTCAGGG AGCCTCCGCC ATCCCCACTG CCATCGATTT CACTCTATGG
AGGCAATCCA TTAAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTCCCCTGAC
CCAGAGTCTG TGTTCCGAGC AGCACTCAGT AAGAAGGTGG CTGACTTGAT TCATTTTCTG
CTCCTCAAGT ATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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