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MAGEA5 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MAGEA5
Entrez Gene ID 4104
Full Name melanoma antigen family A5
Synonyms CT1.5, MAGE5, MAGEA4
General protein information
Preferred Names
melanoma-associated antigen 5
Names
melanoma-associated antigen 5
MAGE-5 antigen
MAGE-5a antigen
MAGE-5b antigen
cancer/testis antigen 1.5
cancer/testis antigen family 1, member 5
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xq28

Summary This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This MAGEA gene encodes a protein that is C-terminally truncated compared to other family members, and this gene can be alternatively interpreted to be a pseudogene. The protein is represented in this Gene record in accordance with the assumed protein-coding status defined in the literature. Read-through transcription exists between this gene and the upstream melanoma antigen family A, 10 (MAGEA10) gene. [provided by RefSeq, Oct 2011]. lac of sum
Disorder MIM:

300340


mRNA and Protein(s)

mRNA Protein Name
NM_021049 NP_066387 melanoma-associated antigen 5


Homo sapiens (human) MAGEA10-MAGEA5 NP_001191740.1
Homo sapiens (human) MAGEA5 NP_066387.1

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MAGEA5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGEA5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu08405 NM_021049 Homo sapiens melanoma antigen family A5 (MAGEA5), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu08405
Clone ID Related Accession (Same CDS sequence) NM_001204811 , NM_021049
Accession Version NM_021049.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 375bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC109187.1, AF274856.3 and AI200443.1. This sequence is a reference standard in the RefSeqGene project. On Oct 18, 2011 this sequence version replaced gi:58530874. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC109187.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2148874, SAMEA2149398 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
ATGTCTCTTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA CACCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGTGCAGGCT GCCACTACTG AGGAGCAGGA GGCTGTGTCC
TCCTCCTCTC CTCTGGTCCC AGGCACCCTG GGGGAGGTGC CTGCTGCTGG GTCACCAGGT
CCTCTCAAGA GTCCTCAGGG AGCCTCCGCC ATCCCCACTG CCATCGATTT CACTCTATGG
AGGCAATCCA TTAAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTCCCCTGAC
CCAGAGTCTG TGTTCCGAGC AGCACTCAGT AAGAAGGTGG CTGACTTGAT TCATTTTCTG
CTCCTCAAGT ATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_066387.1
CDS173..547
Misc Feature(1)38..40(+)
Misc Feature(2)179..466(+)
Exon (1)1..20
Gene:MAGEA5
Gene Synonym:
Exon (2)21..107
Gene:MAGEA5
Gene Synonym:
Exon (3)108..1664
Gene:MAGEA5
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_021049
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family A5 (MAGEA5), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_021049

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGTCTCTTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA CACCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGTGCAGGCT GCCACTACTG AGGAGCAGGA GGCTGTGTCC
TCCTCCTCTC CTCTGGTCCC AGGCACCCTG GGGGAGGTGC CTGCTGCTGG GTCACCAGGT
CCTCTCAAGA GTCCTCAGGG AGCCTCCGCC ATCCCCACTG CCATCGATTT CACTCTATGG
AGGCAATCCA TTAAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTCCCCTGAC
CCAGAGTCTG TGTTCCGAGC AGCACTCAGT AAGAAGGTGG CTGACTTGAT TCATTTTCTG
CTCCTCAAGT ATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

MAGE A1-A6 RT-PCR and MAGE A3 and p16 methylation analysis in induced sputum from patients with lung cancer and non-malignant lung diseases
Oncol. Rep. 27 (4), 911-916 (2012)
Shin KC, Lee KH, Lee CH, Shin IH, Suh HS and Jeon CH.


book

Expression of melanoma-associated antigens in oral squamous cell carcinoma
J. Oral Pathol. Med. 37 (2), 88-93 (2008)
Ries J, Vairaktaris E, Mollaoglu N, Wiltfang J, Neukam FW and Nkenke E.


book

Quantitative evaluation of the expression of MAGE genes in tumors by limiting dilution of cDNA libraries
Int. J. Cancer 83 (5), 664-669 (1999)
Serrano A, Lethe B, Delroisse JM, Lurquin C, De Plaen E, Brasseur F, Rimoldi D and Boon T.


book

The melanoma antigen gene (MAGE) family is clustered in the chromosomal band Xq28
Genomics 29 (3), 725-731 (1995)
Rogner UC, Wilke K, Steck E, Korn B and Poustka A.


book

Structure, chromosomal localization, and expression of 12 genes of the MAGE family
Immunogenetics 40 (5), 360-369 (1994)
De Plaen E, Arden K, Traversari C, Gaforio JJ, Szikora JP, De Smet C, Brasseur F, van der Bruggen P, Lethe B and Lurquin C.


 
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