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MAGEA10 melanoma antigen family A10 [Homo sapiens (human)]

Gene Symbol MAGEA10
Entrez Gene ID 4109
Full Name melanoma antigen family A10
Synonyms CT1.10, MAGE10
General protein information
Preferred Names
melanoma-associated antigen 10
Names
melanoma-associated antigen 10
MAGE-10 antigen
cancer/testis antigen 1.10
cancer/testis antigen family 1, member 10
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xq28

Summary This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream melanoma antigen family A, 5 (MAGEA5) gene.[provided by RefSeq, Oct 2011]. lac of sum
Disorder MIM:

300343

Disorder Html:
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu10659 NM_001011543 Homo sapiens melanoma antigen family A10 (MAGEA10), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu10659 NM_021048 Homo sapiens melanoma antigen family A10 (MAGEA10), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu10659 NM_001251828 Homo sapiens melanoma antigen family A10 (MAGEA10), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu10659D
Sequence Information ORF Nucleotide Sequence (Length: 1110bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 10
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK314161.1, CB158974.1, AC116666.2, BC004105.2 and BQ026281.1. On Oct 18, 2011 this sequence version replaced gi:58530862. Summary: This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream melanoma antigen family A, 5 (MAGEA5) gene.[provided by RefSeq, Oct 2011]. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM458898.1, BU167137.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCCTCGAG CTCCAAAGCG TCAGCGCTGC ATGCCTGAAG AAGATCTTCA ATCCCAAAGT 
GAGACACAGG GCCTCGAGGG TGCACAGGCT CCCCTGGCTG TGGAGGAGGA TGCTTCATCA
TCCACTTCCA CCAGCTCCTC TTTTCCATCC TCTTTTCCCT CCTCCTCCTC TTCCTCCTCC
TCCTCCTGCT ATCCTCTAAT ACCAAGCACC CCAGAGGAGG TTTCTGCTGA TGATGAGACA
CCAAATCCTC CCCAGAGTGC TCAGATAGCC TGCTCCTCCC CCTCGGTCGT TGCTTCCCTT
CCATTAGATC AATCTGATGA GGGCTCCAGC AGCCAAAAGG AGGAGAGTCC AAGCACCCTA
CAGGTCCTGC CAGACAGTGA GTCTTTACCC AGAAGTGAGA TAGATGAAAA GGTGACTGAT
TTGGTGCAGT TTCTGCTCTT CAAGTATCAA ATGAAGGAGC CGATCACAAA GGCAGAAATA
CTGGAGAGTG TCATAAGAAA TTATGAAGAC CACTTCCCTT TGTTGTTTAG TGAAGCCTCC
GAGTGCATGC TGCTGGTCTT TGGCATTGAT GTAAAGGAAG TGGATCCCAC TGGCCACTCC
TTTGTCCTTG TCACCTCCCT GGGCCTCACC TATGATGGGA TGCTGAGTGA TGTCCAGAGC
ATGCCCAAGA CTGGCATTCT CATACTTATC CTAAGCATAA TCTTCATAGA GGGCTACTGC
ACCCCTGAGG AGGTCATCTG GGAAGCACTG AATATGATGG GGCTGTATGA TGGGATGGAG
CACCTCATTT ATGGGGAGCC CAGGAAGCTG CTCACCCAAG ATTGGGTGCA GGAAAACTAC
CTGGAGTACC GGCAGGTGCC TGGCAGTGAT CCTGCACGGT ATGAGTTTCT GTGGGGTCCA
AGGGCTCATG CTGAAATTAG GAAGATGAGT CTCCTGAAAT TTTTGGCCAA GGTAAATGGG
AGTGATCCAA GATCCTTCCC ACTGTGGTAT GAGGAGGCTT TGAAAGATGA GGAAGAGAGA
GCCCAGGACA GAATTGCCAC CACAGATGAT ACTACTGCCA TGGCCAGTGC AAGTTCTAGC
GCTACAGGTA GCTTCTCCTA CCCTGAATAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001011543.2
CDS434..1543
Misc Feature(1)224..226(+)
Misc Feature(2)440..793(+)
Misc Feature(3)854..1366(+)
Exon (1)1..104
Gene:MAGEA10
Gene Synonym:
Exon (2)105..203
Gene:MAGEA10
Gene Synonym:
Exon (3)204..294
Gene:MAGEA10
Gene Synonym:
Exon (4)295..368
Gene:MAGEA10
Gene Synonym:
Exon (5)369..2744
Gene:MAGEA10
Gene Synonym:
Translation
Position Chain Variation Link
78 78 c, t dbSNP:764505905
166 166 a, g dbSNP:777960527
181 181 c, t dbSNP:772633959
182 182 a, g dbSNP:758519631
183 183 c, t dbSNP:72614771
185 185 a, g dbSNP:650459
187 187 a, t dbSNP:683114
203 203 a, g dbSNP:778639496
222 222 a, g dbSNP:751001093
248 248 c, t dbSNP:763494940
315 315 a, g dbSNP:369373758
316 316 a, c dbSNP:772764022
319 319 c, g dbSNP:775215340
385 385 c, t dbSNP:199683050
386 386 a, g dbSNP:202079307
387 387 c, t dbSNP:768227548
392 392 a, g dbSNP:749763998
398 398 c, t dbSNP:780737224
410 410 c, t dbSNP:756524377
427 427 a, c dbSNP:750875467
437 437 c, t dbSNP:781471162
438 438 c, t dbSNP:757353420
440 440 c, t dbSNP:183488441
441 441 a, g dbSNP:148914808
452 452 c, t dbSNP:376230948
453 453 a, g dbSNP:145553450
458 458 c, t dbSNP:766357263
459 459 a, g dbSNP:760453323
471 471 a, c dbSNP:773061544
475 475 -, aga dbSNP:756690955
476 476 c, g dbSNP:771907807
478 478 c, t dbSNP:572703181
484 484 a, g dbSNP:774076552
488 488 c, g dbSNP:768011654
502 502 g, t dbSNP:776537677
504 504 a, g dbSNP:748856300
508 508 c, g dbSNP:770828622
509 509 a, g dbSNP:372755429
516 516 c, g dbSNP:746380725
545 545 c, g dbSNP:781351693
546 546 c, t dbSNP:143413048
556 556 c, t dbSNP:751689489
564 564 c, t dbSNP:199974088
570 570 a, c dbSNP:773216378
580 580 a, c dbSNP:758455205
591 591 -, atcctcttttcc dbSNP:750860953
591 591 c, t dbSNP:369588146
598 598 c, g dbSNP:752835890
601 601 -, ctc dbSNP:767951816
604 604 -, ttcctcctcctcctc dbSNP:757511208
606 606 c, t dbSNP:766302328
609 609 a, c, t dbSNP:750389786
613 613 a, c dbSNP:767169087
615 615 -, cctcct dbSNP:751797072
618 618 -, cct dbSNP:763160116
620 620 -, cct dbSNP:764239151
629 629 c, t dbSNP:761700669
635 635 c, t dbSNP:773844290
640 640 a, c dbSNP:768413554
658 658 c, t dbSNP:762547856
660 660 a, c dbSNP:775072205
675 675 c, t dbSNP:769440760
683 683 a, c dbSNP:746325749
685 685 c, t dbSNP:777222995
690 690 c, g dbSNP:376173074
702 702 a, c, t dbSNP:777848689
704 704 c, t dbSNP:200703002
705 705 g, t dbSNP:748376871
711 711 c, t dbSNP:201745611
715 715 -, c dbSNP:775608425
715 715 c, g, t dbSNP:750288203
717 717 c, t dbSNP:748262488
718 718 a, g dbSNP:139926957
722 722 a, g dbSNP:751437238
726 726 a, c dbSNP:763984309
741 741 a, g dbSNP:755172887
745 745 -, a dbSNP:765210005
747 747 c, t dbSNP:267606385
750 750 a, t dbSNP:762635118
752 752 a, g dbSNP:775301637
764 764 a, g dbSNP:373155792
768 768 a, g dbSNP:764797687
771 771 a, g dbSNP:759153084
772 772 g, t dbSNP:776976782
783 783 a, c dbSNP:374907730
790 790 c, t dbSNP:747334799
791 791 c, g dbSNP:773562498
803 803 c, g dbSNP:369858882
834 834 c, t dbSNP:377180452
844 844 a, g dbSNP:749548772
857 857 c, g dbSNP:755021278
871 871 c, t dbSNP:749434711
874 874 c, t dbSNP:780986979
882 882 a, g dbSNP:757151645
885 885 c, t dbSNP:751464289
887 887 a, g dbSNP:763931203
894 894 c, t dbSNP:758297801
895 895 a, g, t dbSNP:146444641
897 897 c, t dbSNP:758952037
914 914 c, t dbSNP:193021490
917 917 a, g dbSNP:776275648
920 920 a, c dbSNP:756555479
921 921 c, g dbSNP:761189502
926 926 a, g dbSNP:773732678
930 930 a, g dbSNP:210585
934 934 g, t dbSNP:762205693
939 939 a, g dbSNP:774535111
949 949 a, c dbSNP:768932478
956 956 c, t dbSNP:768072387
966 966 a, g, t dbSNP:149860947
973 973 c, t dbSNP:138991758
974 974 a, g dbSNP:777894946
983 983 c, t dbSNP:150729017
990 990 g, t dbSNP:752593390
997 997 c, t dbSNP:778707411
1003 1003 c, t dbSNP:754596795
1006 1006 a, c, t dbSNP:766030985
1010 1010 a, g dbSNP:760135991
1016 1016 g, t dbSNP:750900691
1031 1031 c, t dbSNP:768061289
1051 1051 c, t dbSNP:746306839
1063 1063 a, c dbSNP:369416770
1080 1080 a, g dbSNP:142008219
1086 1086 c, t dbSNP:763184135
1093 1093 c, t dbSNP:775605412
1111 1111 c, t dbSNP:769882541
1122 1122 -, tccta dbSNP:760709707
1122 1122 c, t dbSNP:745981774
1130 1130 a, t dbSNP:777835646
1132 1132 a, g dbSNP:772198855
1133 1133 a, g dbSNP:210586
1142 1142 c, g dbSNP:200325353
1148 1148 c, t dbSNP:754755138
1158 1158 c, g dbSNP:753491437
1169 1169 a, t dbSNP:772987237
1196 1196 c, g dbSNP:755628514
1198 1198 a, g dbSNP:749993834
1201 1201 c, t dbSNP:767018212
1206 1206 g, t dbSNP:143177343
1213 1213 a, g dbSNP:752081589
1215 1215 a, g dbSNP:764477700
1228 1228 a, g dbSNP:267606384
1231 1231 a, g dbSNP:763264787
1246 1246 c, t dbSNP:139266234
1250 1250 a, c dbSNP:765431558
1255 1255 c, t dbSNP:376509634
1261 1261 a, g dbSNP:776788698
1273 1273 c, t dbSNP:771152326
1276 1276 g, t dbSNP:748052884
1283 1283 c, t dbSNP:146467787
1284 1284 a, g dbSNP:768468815
1287 1287 a, g dbSNP:202016657
1290 1290 g, t dbSNP:201253472
1292 1292 c, g dbSNP:755790460
1294 1294 c, t dbSNP:745532310
1299 1299 c, g dbSNP:371673293
1300 1300 g, t dbSNP:756789226
1301 1301 a, g dbSNP:752028683
1303 1303 c, t dbSNP:368166773
1304 1304 a, c dbSNP:758774248
1310 1310 c, t dbSNP:149591320
1311 1311 a, g dbSNP:200418003
1314 1314 a, g dbSNP:759778284
1318 1318 a, g dbSNP:373631896
1337 1337 c, g dbSNP:766529255
1344 1344 c, t dbSNP:760879446
1357 1357 a, g dbSNP:774325402
1360 1360 a, g dbSNP:768534650
1363 1363 c, t dbSNP:202105507
1366 1366 c, t dbSNP:371698499
1389 1389 a, g dbSNP:775088145
1391 1391 g, t dbSNP:769599579
1435 1435 c, g dbSNP:745481385
1442 1442 a, g dbSNP:780861236
1447 1447 a, c dbSNP:756735659
1475 1475 a, g dbSNP:751679256
1486 1486 a, c, t dbSNP:778334853
1505 1505 a, g dbSNP:367772567
1506 1506 c, g dbSNP:199597806
1508 1508 -, a dbSNP:772983177
1513 1513 a, c, t dbSNP:138141548
1514 1514 a, g dbSNP:201636610
1518 1518 c, t dbSNP:764386887
1533 1533 a, c dbSNP:780333247
1545 1545 a, g dbSNP:766758155
1546 1546 g, t dbSNP:760826402
1554 1554 a, t dbSNP:374443836
1557 1557 c, t dbSNP:371059360
1559 1559 -, t dbSNP:771924321
1568 1568 g, t dbSNP:762749424
1584 1584 a, t dbSNP:775529298
1592 1592 a, g dbSNP:769545496
1635 1635 -, a dbSNP:34592629
1635 1635 -, a dbSNP:34509899
1644 1644 c, t dbSNP:746275103
1683 1683 a, g dbSNP:781500862
1692 1692 a, g dbSNP:757815499
1750 1750 a, g dbSNP:758730725
1755 1755 g, t dbSNP:752180238
1816 1816 c, t dbSNP:183526224
1911 1911 c, t dbSNP:753677144
1941 1941 c, t dbSNP:375526640
1959 1959 a, g dbSNP:192018151
1960 1960 a, g dbSNP:187259697
1971 1971 a, c dbSNP:181728158
1973 1973 a, g dbSNP:766334189
1974 1974 c, t dbSNP:750213741
2085 2085 g, t dbSNP:12844594
2086 2086 a, g dbSNP:190079936
2114 2114 c, t dbSNP:760531651
2117 2117 g, t dbSNP:210587
2124 2124 -, tt dbSNP:761732676
2173 2173 c, g, t dbSNP:185629643
2174 2174 a, g dbSNP:181180625
2191 2191 a, g dbSNP:750259597
2247 2247 a, g dbSNP:763088752
2277 2277 c, t dbSNP:775534050
2285 2285 a, g dbSNP:143950968
2308 2308 c, t dbSNP:188251781
2336 2336 c, t dbSNP:1907602
2352 2352 a, g dbSNP:771481477
2362 2362 a, c dbSNP:147496083
2365 2365 a, g dbSNP:778429386
2478 2478 c, t dbSNP:540649581
2531 2531 a, g dbSNP:773892363
2671 2671 a, g dbSNP:543345906
2676 2676 c, t dbSNP:183572820
2683 2683 a, g dbSNP:768294201
2708 2708 a, g dbSNP:779481884
2720 2720 c, t dbSNP:755763489
2721 2721 g, t dbSNP:762652265

Target ORF information:

RefSeq Version NM_001011543
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family A10 (MAGEA10), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001011543

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCCTCGAG CTCCAAAGCG TCAGCGCTGC ATGCCTGAAG AAGATCTTCA ATCCCAAAGT 
GAGACACAGG GCCTCGAGGG TGCACAGGCT CCCCTGGCTG TGGAGGAGGA TGCTTCATCA
TCCACTTCCA CCAGCTCCTC TTTTCCATCC TCTTTTCCCT CCTCCTCCTC TTCCTCCTCC
TCCTCCTGCT ATCCTCTAAT ACCAAGCACC CCAGAGGAGG TTTCTGCTGA TGATGAGACA
CCAAATCCTC CCCAGAGTGC TCAGATAGCC TGCTCCTCCC CCTCGGTCGT TGCTTCCCTT
CCATTAGATC AATCTGATGA GGGCTCCAGC AGCCAAAAGG AGGAGAGTCC AAGCACCCTA
CAGGTCCTGC CAGACAGTGA GTCTTTACCC AGAAGTGAGA TAGATGAAAA GGTGACTGAT
TTGGTGCAGT TTCTGCTCTT CAAGTATCAA ATGAAGGAGC CGATCACAAA GGCAGAAATA
CTGGAGAGTG TCATAAGAAA TTATGAAGAC CACTTCCCTT TGTTGTTTAG TGAAGCCTCC
GAGTGCATGC TGCTGGTCTT TGGCATTGAT GTAAAGGAAG TGGATCCCAC TGGCCACTCC
TTTGTCCTTG TCACCTCCCT GGGCCTCACC TATGATGGGA TGCTGAGTGA TGTCCAGAGC
ATGCCCAAGA CTGGCATTCT CATACTTATC CTAAGCATAA TCTTCATAGA GGGCTACTGC
ACCCCTGAGG AGGTCATCTG GGAAGCACTG AATATGATGG GGCTGTATGA TGGGATGGAG
CACCTCATTT ATGGGGAGCC CAGGAAGCTG CTCACCCAAG ATTGGGTGCA GGAAAACTAC
CTGGAGTACC GGCAGGTGCC TGGCAGTGAT CCTGCACGGT ATGAGTTTCT GTGGGGTCCA
AGGGCTCATG CTGAAATTAG GAAGATGAGT CTCCTGAAAT TTTTGGCCAA GGTAAATGGG
AGTGATCCAA GATCCTTCCC ACTGTGGTAT GAGGAGGCTT TGAAAGATGA GGAAGAGAGA
GCCCAGGACA GAATTGCCAC CACAGATGAT ACTACTGCCA TGGCCAGTGC AAGTTCTAGC
GCTACAGGTA GCTTCTCCTA CCCTGAATAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu10659D
Sequence Information ORF Nucleotide Sequence (Length: 1110bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 10
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK314161.1, AC116666.2, BC004105.2 and BQ026281.1. On Oct 18, 2011 this sequence version replaced gi:29029613. Summary: This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream melanoma antigen family A, 5 (MAGEA5) gene.[provided by RefSeq, Oct 2011]. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314161.1, BC004105.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCCTCGAG CTCCAAAGCG TCAGCGCTGC ATGCCTGAAG AAGATCTTCA ATCCCAAAGT 
GAGACACAGG GCCTCGAGGG TGCACAGGCT CCCCTGGCTG TGGAGGAGGA TGCTTCATCA
TCCACTTCCA CCAGCTCCTC TTTTCCATCC TCTTTTCCCT CCTCCTCCTC TTCCTCCTCC
TCCTCCTGCT ATCCTCTAAT ACCAAGCACC CCAGAGGAGG TTTCTGCTGA TGATGAGACA
CCAAATCCTC CCCAGAGTGC TCAGATAGCC TGCTCCTCCC CCTCGGTCGT TGCTTCCCTT
CCATTAGATC AATCTGATGA GGGCTCCAGC AGCCAAAAGG AGGAGAGTCC AAGCACCCTA
CAGGTCCTGC CAGACAGTGA GTCTTTACCC AGAAGTGAGA TAGATGAAAA GGTGACTGAT
TTGGTGCAGT TTCTGCTCTT CAAGTATCAA ATGAAGGAGC CGATCACAAA GGCAGAAATA
CTGGAGAGTG TCATAAGAAA TTATGAAGAC CACTTCCCTT TGTTGTTTAG TGAAGCCTCC
GAGTGCATGC TGCTGGTCTT TGGCATTGAT GTAAAGGAAG TGGATCCCAC TGGCCACTCC
TTTGTCCTTG TCACCTCCCT GGGCCTCACC TATGATGGGA TGCTGAGTGA TGTCCAGAGC
ATGCCCAAGA CTGGCATTCT CATACTTATC CTAAGCATAA TCTTCATAGA GGGCTACTGC
ACCCCTGAGG AGGTCATCTG GGAAGCACTG AATATGATGG GGCTGTATGA TGGGATGGAG
CACCTCATTT ATGGGGAGCC CAGGAAGCTG CTCACCCAAG ATTGGGTGCA GGAAAACTAC
CTGGAGTACC GGCAGGTGCC TGGCAGTGAT CCTGCACGGT ATGAGTTTCT GTGGGGTCCA
AGGGCTCATG CTGAAATTAG GAAGATGAGT CTCCTGAAAT TTTTGGCCAA GGTAAATGGG
AGTGATCCAA GATCCTTCCC ACTGTGGTAT GAGGAGGCTT TGAAAGATGA GGAAGAGAGA
GCCCAGGACA GAATTGCCAC CACAGATGAT ACTACTGCCA TGGCCAGTGC AAGTTCTAGC
GCTACAGGTA GCTTCTCCTA CCCTGAATAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_066386.2
CDS343..1452
Misc Feature(1)190..192(+)
Misc Feature(2)349..702(+)
Misc Feature(3)763..1275(+)
Exon (1)1..104
Gene:MAGEA10
Gene Synonym:
Exon (2)105..203
Gene:MAGEA10
Gene Synonym:
Exon (3)204..277
Gene:MAGEA10
Gene Synonym:
Exon (4)278..2653
Gene:MAGEA10
Gene Synonym:
Translation
Position Chain Variation Link
78 78 c, t dbSNP:764505905
166 166 a, g dbSNP:777960527
181 181 c, t dbSNP:772633959
182 182 a, g dbSNP:758519631
183 183 c, t dbSNP:72614771
185 185 a, g dbSNP:650459
187 187 a, t dbSNP:683114
203 203 a, g dbSNP:778639496
224 224 a, g dbSNP:369373758
225 225 a, c dbSNP:772764022
228 228 c, g dbSNP:775215340
294 294 c, t dbSNP:199683050
295 295 a, g dbSNP:202079307
296 296 c, t dbSNP:768227548
301 301 a, g dbSNP:749763998
307 307 c, t dbSNP:780737224
319 319 c, t dbSNP:756524377
336 336 a, c dbSNP:750875467
346 346 c, t dbSNP:781471162
347 347 c, t dbSNP:757353420
349 349 c, t dbSNP:183488441
350 350 a, g dbSNP:148914808
361 361 c, t dbSNP:376230948
362 362 a, g dbSNP:145553450
367 367 c, t dbSNP:766357263
368 368 a, g dbSNP:760453323
380 380 a, c dbSNP:773061544
384 384 -, aga dbSNP:756690955
385 385 c, g dbSNP:771907807
387 387 c, t dbSNP:572703181
393 393 a, g dbSNP:774076552
397 397 c, g dbSNP:768011654
411 411 g, t dbSNP:776537677
413 413 a, g dbSNP:748856300
417 417 c, g dbSNP:770828622
418 418 a, g dbSNP:372755429
425 425 c, g dbSNP:746380725
454 454 c, g dbSNP:781351693
455 455 c, t dbSNP:143413048
465 465 c, t dbSNP:751689489
473 473 c, t dbSNP:199974088
479 479 a, c dbSNP:773216378
489 489 a, c dbSNP:758455205
500 500 -, atcctcttttcc dbSNP:750860953
500 500 c, t dbSNP:369588146
507 507 c, g dbSNP:752835890
510 510 -, ctc dbSNP:767951816
513 513 -, ttcctcctcctcctc dbSNP:757511208
515 515 c, t dbSNP:766302328
518 518 a, c, t dbSNP:750389786
522 522 a, c dbSNP:767169087
524 524 -, cctcct dbSNP:751797072
527 527 -, cct dbSNP:763160116
529 529 -, cct dbSNP:764239151
538 538 c, t dbSNP:761700669
544 544 c, t dbSNP:773844290
549 549 a, c dbSNP:768413554
567 567 c, t dbSNP:762547856
569 569 a, c dbSNP:775072205
584 584 c, t dbSNP:769440760
592 592 a, c dbSNP:746325749
594 594 c, t dbSNP:777222995
599 599 c, g dbSNP:376173074
611 611 a, c, t dbSNP:777848689
613 613 c, t dbSNP:200703002
614 614 g, t dbSNP:748376871
620 620 c, t dbSNP:201745611
624 624 -, c dbSNP:775608425
624 624 c, g, t dbSNP:750288203
626 626 c, t dbSNP:748262488
627 627 a, g dbSNP:139926957
631 631 a, g dbSNP:751437238
635 635 a, c dbSNP:763984309
650 650 a, g dbSNP:755172887
654 654 -, a dbSNP:765210005
656 656 c, t dbSNP:267606385
659 659 a, t dbSNP:762635118
661 661 a, g dbSNP:775301637
673 673 a, g dbSNP:373155792
677 677 a, g dbSNP:764797687
680 680 a, g dbSNP:759153084
681 681 g, t dbSNP:776976782
692 692 a, c dbSNP:374907730
699 699 c, t dbSNP:747334799
700 700 c, g dbSNP:773562498
712 712 c, g dbSNP:369858882
743 743 c, t dbSNP:377180452
753 753 a, g dbSNP:749548772
766 766 c, g dbSNP:755021278
780 780 c, t dbSNP:749434711
783 783 c, t dbSNP:780986979
791 791 a, g dbSNP:757151645
794 794 c, t dbSNP:751464289
796 796 a, g dbSNP:763931203
803 803 c, t dbSNP:758297801
804 804 a, g, t dbSNP:146444641
806 806 c, t dbSNP:758952037
823 823 c, t dbSNP:193021490
826 826 a, g dbSNP:776275648
829 829 a, c dbSNP:756555479
830 830 c, g dbSNP:761189502
835 835 a, g dbSNP:773732678
839 839 a, g dbSNP:210585
843 843 g, t dbSNP:762205693
848 848 a, g dbSNP:774535111
858 858 a, c dbSNP:768932478
865 865 c, t dbSNP:768072387
875 875 a, g, t dbSNP:149860947
882 882 c, t dbSNP:138991758
883 883 a, g dbSNP:777894946
892 892 c, t dbSNP:150729017
899 899 g, t dbSNP:752593390
906 906 c, t dbSNP:778707411
912 912 c, t dbSNP:754596795
915 915 a, c, t dbSNP:766030985
919 919 a, g dbSNP:760135991
925 925 g, t dbSNP:750900691
940 940 c, t dbSNP:768061289
960 960 c, t dbSNP:746306839
972 972 a, c dbSNP:369416770
989 989 a, g dbSNP:142008219
995 995 c, t dbSNP:763184135
1002 1002 c, t dbSNP:775605412
1020 1020 c, t dbSNP:769882541
1031 1031 -, tccta dbSNP:760709707
1031 1031 c, t dbSNP:745981774
1039 1039 a, t dbSNP:777835646
1041 1041 a, g dbSNP:772198855
1042 1042 a, g dbSNP:210586
1051 1051 c, g dbSNP:200325353
1057 1057 c, t dbSNP:754755138
1067 1067 c, g dbSNP:753491437
1078 1078 a, t dbSNP:772987237
1105 1105 c, g dbSNP:755628514
1107 1107 a, g dbSNP:749993834
1110 1110 c, t dbSNP:767018212
1115 1115 g, t dbSNP:143177343
1122 1122 a, g dbSNP:752081589
1124 1124 a, g dbSNP:764477700
1137 1137 a, g dbSNP:267606384
1140 1140 a, g dbSNP:763264787
1155 1155 c, t dbSNP:139266234
1159 1159 a, c dbSNP:765431558
1164 1164 c, t dbSNP:376509634
1170 1170 a, g dbSNP:776788698
1182 1182 c, t dbSNP:771152326
1185 1185 g, t dbSNP:748052884
1192 1192 c, t dbSNP:146467787
1193 1193 a, g dbSNP:768468815
1196 1196 a, g dbSNP:202016657
1199 1199 g, t dbSNP:201253472
1201 1201 c, g dbSNP:755790460
1203 1203 c, t dbSNP:745532310
1208 1208 c, g dbSNP:371673293
1209 1209 g, t dbSNP:756789226
1210 1210 a, g dbSNP:752028683
1212 1212 c, t dbSNP:368166773
1213 1213 a, c dbSNP:758774248
1219 1219 c, t dbSNP:149591320
1220 1220 a, g dbSNP:200418003
1223 1223 a, g dbSNP:759778284
1227 1227 a, g dbSNP:373631896
1246 1246 c, g dbSNP:766529255
1253 1253 c, t dbSNP:760879446
1266 1266 a, g dbSNP:774325402
1269 1269 a, g dbSNP:768534650
1272 1272 c, t dbSNP:202105507
1275 1275 c, t dbSNP:371698499
1298 1298 a, g dbSNP:775088145
1300 1300 g, t dbSNP:769599579
1344 1344 c, g dbSNP:745481385
1351 1351 a, g dbSNP:780861236
1356 1356 a, c dbSNP:756735659
1384 1384 a, g dbSNP:751679256
1395 1395 a, c, t dbSNP:778334853
1414 1414 a, g dbSNP:367772567
1415 1415 c, g dbSNP:199597806
1417 1417 -, a dbSNP:772983177
1422 1422 a, c, t dbSNP:138141548
1423 1423 a, g dbSNP:201636610
1427 1427 c, t dbSNP:764386887
1442 1442 a, c dbSNP:780333247
1454 1454 a, g dbSNP:766758155
1455 1455 g, t dbSNP:760826402
1463 1463 a, t dbSNP:374443836
1466 1466 c, t dbSNP:371059360
1468 1468 -, t dbSNP:771924321
1477 1477 g, t dbSNP:762749424
1493 1493 a, t dbSNP:775529298
1501 1501 a, g dbSNP:769545496
1544 1544 -, a dbSNP:34592629
1544 1544 -, a dbSNP:34509899
1553 1553 c, t dbSNP:746275103
1592 1592 a, g dbSNP:781500862
1601 1601 a, g dbSNP:757815499
1659 1659 a, g dbSNP:758730725
1664 1664 g, t dbSNP:752180238
1725 1725 c, t dbSNP:183526224
1820 1820 c, t dbSNP:753677144
1850 1850 c, t dbSNP:375526640
1868 1868 a, g dbSNP:192018151
1869 1869 a, g dbSNP:187259697
1880 1880 a, c dbSNP:181728158
1882 1882 a, g dbSNP:766334189
1883 1883 c, t dbSNP:750213741
1994 1994 g, t dbSNP:12844594
1995 1995 a, g dbSNP:190079936
2023 2023 c, t dbSNP:760531651
2026 2026 g, t dbSNP:210587
2033 2033 -, tt dbSNP:761732676
2082 2082 c, g, t dbSNP:185629643
2083 2083 a, g dbSNP:181180625
2100 2100 a, g dbSNP:750259597
2156 2156 a, g dbSNP:763088752
2186 2186 c, t dbSNP:775534050
2194 2194 a, g dbSNP:143950968
2217 2217 c, t dbSNP:188251781
2245 2245 c, t dbSNP:1907602
2261 2261 a, g dbSNP:771481477
2271 2271 a, c dbSNP:147496083
2274 2274 a, g dbSNP:778429386
2387 2387 c, t dbSNP:540649581
2440 2440 a, g dbSNP:773892363
2580 2580 a, g dbSNP:543345906
2585 2585 c, t dbSNP:183572820
2592 2592 a, g dbSNP:768294201
2617 2617 a, g dbSNP:779481884
2629 2629 c, t dbSNP:755763489
2630 2630 g, t dbSNP:762652265

Target ORF information:

RefSeq Version NM_021048
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family A10 (MAGEA10), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_021048

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCCTCGAG CTCCAAAGCG TCAGCGCTGC ATGCCTGAAG AAGATCTTCA ATCCCAAAGT 
GAGACACAGG GCCTCGAGGG TGCACAGGCT CCCCTGGCTG TGGAGGAGGA TGCTTCATCA
TCCACTTCCA CCAGCTCCTC TTTTCCATCC TCTTTTCCCT CCTCCTCCTC TTCCTCCTCC
TCCTCCTGCT ATCCTCTAAT ACCAAGCACC CCAGAGGAGG TTTCTGCTGA TGATGAGACA
CCAAATCCTC CCCAGAGTGC TCAGATAGCC TGCTCCTCCC CCTCGGTCGT TGCTTCCCTT
CCATTAGATC AATCTGATGA GGGCTCCAGC AGCCAAAAGG AGGAGAGTCC AAGCACCCTA
CAGGTCCTGC CAGACAGTGA GTCTTTACCC AGAAGTGAGA TAGATGAAAA GGTGACTGAT
TTGGTGCAGT TTCTGCTCTT CAAGTATCAA ATGAAGGAGC CGATCACAAA GGCAGAAATA
CTGGAGAGTG TCATAAGAAA TTATGAAGAC CACTTCCCTT TGTTGTTTAG TGAAGCCTCC
GAGTGCATGC TGCTGGTCTT TGGCATTGAT GTAAAGGAAG TGGATCCCAC TGGCCACTCC
TTTGTCCTTG TCACCTCCCT GGGCCTCACC TATGATGGGA TGCTGAGTGA TGTCCAGAGC
ATGCCCAAGA CTGGCATTCT CATACTTATC CTAAGCATAA TCTTCATAGA GGGCTACTGC
ACCCCTGAGG AGGTCATCTG GGAAGCACTG AATATGATGG GGCTGTATGA TGGGATGGAG
CACCTCATTT ATGGGGAGCC CAGGAAGCTG CTCACCCAAG ATTGGGTGCA GGAAAACTAC
CTGGAGTACC GGCAGGTGCC TGGCAGTGAT CCTGCACGGT ATGAGTTTCT GTGGGGTCCA
AGGGCTCATG CTGAAATTAG GAAGATGAGT CTCCTGAAAT TTTTGGCCAA GGTAAATGGG
AGTGATCCAA GATCCTTCCC ACTGTGGTAT GAGGAGGCTT TGAAAGATGA GGAAGAGAGA
GCCCAGGACA GAATTGCCAC CACAGATGAT ACTACTGCCA TGGCCAGTGC AAGTTCTAGC
GCTACAGGTA GCTTCTCCTA CCCTGAATAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu10659D
Sequence Information ORF Nucleotide Sequence (Length: 1110bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 10
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK314161.1, DA827990.1, AC116666.2, BC004105.2 and BQ026281.1. Summary: This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream melanoma antigen family A, 5 (MAGEA5) gene.[provided by RefSeq, Oct 2011]. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DA827990.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCCTCGAG CTCCAAAGCG TCAGCGCTGC ATGCCTGAAG AAGATCTTCA ATCCCAAAGT 
GAGACACAGG GCCTCGAGGG TGCACAGGCT CCCCTGGCTG TGGAGGAGGA TGCTTCATCA
TCCACTTCCA CCAGCTCCTC TTTTCCATCC TCTTTTCCCT CCTCCTCCTC TTCCTCCTCC
TCCTCCTGCT ATCCTCTAAT ACCAAGCACC CCAGAGGAGG TTTCTGCTGA TGATGAGACA
CCAAATCCTC CCCAGAGTGC TCAGATAGCC TGCTCCTCCC CCTCGGTCGT TGCTTCCCTT
CCATTAGATC AATCTGATGA GGGCTCCAGC AGCCAAAAGG AGGAGAGTCC AAGCACCCTA
CAGGTCCTGC CAGACAGTGA GTCTTTACCC AGAAGTGAGA TAGATGAAAA GGTGACTGAT
TTGGTGCAGT TTCTGCTCTT CAAGTATCAA ATGAAGGAGC CGATCACAAA GGCAGAAATA
CTGGAGAGTG TCATAAGAAA TTATGAAGAC CACTTCCCTT TGTTGTTTAG TGAAGCCTCC
GAGTGCATGC TGCTGGTCTT TGGCATTGAT GTAAAGGAAG TGGATCCCAC TGGCCACTCC
TTTGTCCTTG TCACCTCCCT GGGCCTCACC TATGATGGGA TGCTGAGTGA TGTCCAGAGC
ATGCCCAAGA CTGGCATTCT CATACTTATC CTAAGCATAA TCTTCATAGA GGGCTACTGC
ACCCCTGAGG AGGTCATCTG GGAAGCACTG AATATGATGG GGCTGTATGA TGGGATGGAG
CACCTCATTT ATGGGGAGCC CAGGAAGCTG CTCACCCAAG ATTGGGTGCA GGAAAACTAC
CTGGAGTACC GGCAGGTGCC TGGCAGTGAT CCTGCACGGT ATGAGTTTCT GTGGGGTCCA
AGGGCTCATG CTGAAATTAG GAAGATGAGT CTCCTGAAAT TTTTGGCCAA GGTAAATGGG
AGTGATCCAA GATCCTTCCC ACTGTGGTAT GAGGAGGCTT TGAAAGATGA GGAAGAGAGA
GCCCAGGACA GAATTGCCAC CACAGATGAT ACTACTGCCA TGGCCAGTGC AAGTTCTAGC
GCTACAGGTA GCTTCTCCTA CCCTGAATAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001238757.1
CDS362..1471
Misc Feature(1)110..112(+)
Misc Feature(2)368..721(+)
Misc Feature(3)782..1294(+)
Exon (1)1..104
Gene:MAGEA10
Gene Synonym:
Exon (2)105..203
Gene:MAGEA10
Gene Synonym:
Exon (3)204..222
Gene:MAGEA10
Gene Synonym:
Exon (4)223..296
Gene:MAGEA10
Gene Synonym:
Exon (5)297..2672
Gene:MAGEA10
Gene Synonym:
Translation
Position Chain Variation Link
78 78 c, t dbSNP:764505905
166 166 a, g dbSNP:777960527
181 181 c, t dbSNP:772633959
182 182 a, g dbSNP:758519631
183 183 c, t dbSNP:72614771
185 185 a, g dbSNP:650459
187 187 a, t dbSNP:683114
203 203 a, g dbSNP:778639496
222 222 a, g dbSNP:751001093
243 243 a, g dbSNP:369373758
244 244 a, c dbSNP:772764022
247 247 c, g dbSNP:775215340
313 313 c, t dbSNP:199683050
314 314 a, g dbSNP:202079307
315 315 c, t dbSNP:768227548
320 320 a, g dbSNP:749763998
326 326 c, t dbSNP:780737224
338 338 c, t dbSNP:756524377
355 355 a, c dbSNP:750875467
365 365 c, t dbSNP:781471162
366 366 c, t dbSNP:757353420
368 368 c, t dbSNP:183488441
369 369 a, g dbSNP:148914808
380 380 c, t dbSNP:376230948
381 381 a, g dbSNP:145553450
386 386 c, t dbSNP:766357263
387 387 a, g dbSNP:760453323
399 399 a, c dbSNP:773061544
403 403 -, aga dbSNP:756690955
404 404 c, g dbSNP:771907807
406 406 c, t dbSNP:572703181
412 412 a, g dbSNP:774076552
416 416 c, g dbSNP:768011654
430 430 g, t dbSNP:776537677
432 432 a, g dbSNP:748856300
436 436 c, g dbSNP:770828622
437 437 a, g dbSNP:372755429
444 444 c, g dbSNP:746380725
473 473 c, g dbSNP:781351693
474 474 c, t dbSNP:143413048
484 484 c, t dbSNP:751689489
492 492 c, t dbSNP:199974088
498 498 a, c dbSNP:773216378
508 508 a, c dbSNP:758455205
519 519 -, atcctcttttcc dbSNP:750860953
519 519 c, t dbSNP:369588146
526 526 c, g dbSNP:752835890
529 529 -, ctc dbSNP:767951816
532 532 -, ttcctcctcctcctc dbSNP:757511208
534 534 c, t dbSNP:766302328
537 537 a, c, t dbSNP:750389786
541 541 a, c dbSNP:767169087
543 543 -, cctcct dbSNP:751797072
546 546 -, cct dbSNP:763160116
548 548 -, cct dbSNP:764239151
557 557 c, t dbSNP:761700669
563 563 c, t dbSNP:773844290
568 568 a, c dbSNP:768413554
586 586 c, t dbSNP:762547856
588 588 a, c dbSNP:775072205
603 603 c, t dbSNP:769440760
611 611 a, c dbSNP:746325749
613 613 c, t dbSNP:777222995
618 618 c, g dbSNP:376173074
630 630 a, c, t dbSNP:777848689
632 632 c, t dbSNP:200703002
633 633 g, t dbSNP:748376871
639 639 c, t dbSNP:201745611
643 643 -, c dbSNP:775608425
643 643 c, g, t dbSNP:750288203
645 645 c, t dbSNP:748262488
646 646 a, g dbSNP:139926957
650 650 a, g dbSNP:751437238
654 654 a, c dbSNP:763984309
669 669 a, g dbSNP:755172887
673 673 -, a dbSNP:765210005
675 675 c, t dbSNP:267606385
678 678 a, t dbSNP:762635118
680 680 a, g dbSNP:775301637
692 692 a, g dbSNP:373155792
696 696 a, g dbSNP:764797687
699 699 a, g dbSNP:759153084
700 700 g, t dbSNP:776976782
711 711 a, c dbSNP:374907730
718 718 c, t dbSNP:747334799
719 719 c, g dbSNP:773562498
731 731 c, g dbSNP:369858882
762 762 c, t dbSNP:377180452
772 772 a, g dbSNP:749548772
785 785 c, g dbSNP:755021278
799 799 c, t dbSNP:749434711
802 802 c, t dbSNP:780986979
810 810 a, g dbSNP:757151645
813 813 c, t dbSNP:751464289
815 815 a, g dbSNP:763931203
822 822 c, t dbSNP:758297801
823 823 a, g, t dbSNP:146444641
825 825 c, t dbSNP:758952037
842 842 c, t dbSNP:193021490
845 845 a, g dbSNP:776275648
848 848 a, c dbSNP:756555479
849 849 c, g dbSNP:761189502
854 854 a, g dbSNP:773732678
858 858 a, g dbSNP:210585
862 862 g, t dbSNP:762205693
867 867 a, g dbSNP:774535111
877 877 a, c dbSNP:768932478
884 884 c, t dbSNP:768072387
894 894 a, g, t dbSNP:149860947
901 901 c, t dbSNP:138991758
902 902 a, g dbSNP:777894946
911 911 c, t dbSNP:150729017
918 918 g, t dbSNP:752593390
925 925 c, t dbSNP:778707411
931 931 c, t dbSNP:754596795
934 934 a, c, t dbSNP:766030985
938 938 a, g dbSNP:760135991
944 944 g, t dbSNP:750900691
959 959 c, t dbSNP:768061289
979 979 c, t dbSNP:746306839
991 991 a, c dbSNP:369416770
1008 1008 a, g dbSNP:142008219
1014 1014 c, t dbSNP:763184135
1021 1021 c, t dbSNP:775605412
1039 1039 c, t dbSNP:769882541
1050 1050 -, tccta dbSNP:760709707
1050 1050 c, t dbSNP:745981774
1058 1058 a, t dbSNP:777835646
1060 1060 a, g dbSNP:772198855
1061 1061 a, g dbSNP:210586
1070 1070 c, g dbSNP:200325353
1076 1076 c, t dbSNP:754755138
1086 1086 c, g dbSNP:753491437
1097 1097 a, t dbSNP:772987237
1124 1124 c, g dbSNP:755628514
1126 1126 a, g dbSNP:749993834
1129 1129 c, t dbSNP:767018212
1134 1134 g, t dbSNP:143177343
1141 1141 a, g dbSNP:752081589
1143 1143 a, g dbSNP:764477700
1156 1156 a, g dbSNP:267606384
1159 1159 a, g dbSNP:763264787
1174 1174 c, t dbSNP:139266234
1178 1178 a, c dbSNP:765431558
1183 1183 c, t dbSNP:376509634
1189 1189 a, g dbSNP:776788698
1201 1201 c, t dbSNP:771152326
1204 1204 g, t dbSNP:748052884
1211 1211 c, t dbSNP:146467787
1212 1212 a, g dbSNP:768468815
1215 1215 a, g dbSNP:202016657
1218 1218 g, t dbSNP:201253472
1220 1220 c, g dbSNP:755790460
1222 1222 c, t dbSNP:745532310
1227 1227 c, g dbSNP:371673293
1228 1228 g, t dbSNP:756789226
1229 1229 a, g dbSNP:752028683
1231 1231 c, t dbSNP:368166773
1232 1232 a, c dbSNP:758774248
1238 1238 c, t dbSNP:149591320
1239 1239 a, g dbSNP:200418003
1242 1242 a, g dbSNP:759778284
1246 1246 a, g dbSNP:373631896
1265 1265 c, g dbSNP:766529255
1272 1272 c, t dbSNP:760879446
1285 1285 a, g dbSNP:774325402
1288 1288 a, g dbSNP:768534650
1291 1291 c, t dbSNP:202105507
1294 1294 c, t dbSNP:371698499
1317 1317 a, g dbSNP:775088145
1319 1319 g, t dbSNP:769599579
1363 1363 c, g dbSNP:745481385
1370 1370 a, g dbSNP:780861236
1375 1375 a, c dbSNP:756735659
1403 1403 a, g dbSNP:751679256
1414 1414 a, c, t dbSNP:778334853
1433 1433 a, g dbSNP:367772567
1434 1434 c, g dbSNP:199597806
1436 1436 -, a dbSNP:772983177
1441 1441 a, c, t dbSNP:138141548
1442 1442 a, g dbSNP:201636610
1446 1446 c, t dbSNP:764386887
1461 1461 a, c dbSNP:780333247
1473 1473 a, g dbSNP:766758155
1474 1474 g, t dbSNP:760826402
1482 1482 a, t dbSNP:374443836
1485 1485 c, t dbSNP:371059360
1487 1487 -, t dbSNP:771924321
1496 1496 g, t dbSNP:762749424
1512 1512 a, t dbSNP:775529298
1520 1520 a, g dbSNP:769545496
1563 1563 -, a dbSNP:34592629
1563 1563 -, a dbSNP:34509899
1572 1572 c, t dbSNP:746275103
1611 1611 a, g dbSNP:781500862
1620 1620 a, g dbSNP:757815499
1678 1678 a, g dbSNP:758730725
1683 1683 g, t dbSNP:752180238
1744 1744 c, t dbSNP:183526224
1839 1839 c, t dbSNP:753677144
1869 1869 c, t dbSNP:375526640
1887 1887 a, g dbSNP:192018151
1888 1888 a, g dbSNP:187259697
1899 1899 a, c dbSNP:181728158
1901 1901 a, g dbSNP:766334189
1902 1902 c, t dbSNP:750213741
2013 2013 g, t dbSNP:12844594
2014 2014 a, g dbSNP:190079936
2042 2042 c, t dbSNP:760531651
2045 2045 g, t dbSNP:210587
2052 2052 -, tt dbSNP:761732676
2101 2101 c, g, t dbSNP:185629643
2102 2102 a, g dbSNP:181180625
2119 2119 a, g dbSNP:750259597
2175 2175 a, g dbSNP:763088752
2205 2205 c, t dbSNP:775534050
2213 2213 a, g dbSNP:143950968
2236 2236 c, t dbSNP:188251781
2264 2264 c, t dbSNP:1907602
2280 2280 a, g dbSNP:771481477
2290 2290 a, c dbSNP:147496083
2293 2293 a, g dbSNP:778429386
2406 2406 c, t dbSNP:540649581
2459 2459 a, g dbSNP:773892363
2599 2599 a, g dbSNP:543345906
2604 2604 c, t dbSNP:183572820
2611 2611 a, g dbSNP:768294201
2636 2636 a, g dbSNP:779481884
2648 2648 c, t dbSNP:755763489
2649 2649 g, t dbSNP:762652265

Target ORF information:

RefSeq Version NM_001251828
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family A10 (MAGEA10), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001251828

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCCTCGAG CTCCAAAGCG TCAGCGCTGC ATGCCTGAAG AAGATCTTCA ATCCCAAAGT 
GAGACACAGG GCCTCGAGGG TGCACAGGCT CCCCTGGCTG TGGAGGAGGA TGCTTCATCA
TCCACTTCCA CCAGCTCCTC TTTTCCATCC TCTTTTCCCT CCTCCTCCTC TTCCTCCTCC
TCCTCCTGCT ATCCTCTAAT ACCAAGCACC CCAGAGGAGG TTTCTGCTGA TGATGAGACA
CCAAATCCTC CCCAGAGTGC TCAGATAGCC TGCTCCTCCC CCTCGGTCGT TGCTTCCCTT
CCATTAGATC AATCTGATGA GGGCTCCAGC AGCCAAAAGG AGGAGAGTCC AAGCACCCTA
CAGGTCCTGC CAGACAGTGA GTCTTTACCC AGAAGTGAGA TAGATGAAAA GGTGACTGAT
TTGGTGCAGT TTCTGCTCTT CAAGTATCAA ATGAAGGAGC CGATCACAAA GGCAGAAATA
CTGGAGAGTG TCATAAGAAA TTATGAAGAC CACTTCCCTT TGTTGTTTAG TGAAGCCTCC
GAGTGCATGC TGCTGGTCTT TGGCATTGAT GTAAAGGAAG TGGATCCCAC TGGCCACTCC
TTTGTCCTTG TCACCTCCCT GGGCCTCACC TATGATGGGA TGCTGAGTGA TGTCCAGAGC
ATGCCCAAGA CTGGCATTCT CATACTTATC CTAAGCATAA TCTTCATAGA GGGCTACTGC
ACCCCTGAGG AGGTCATCTG GGAAGCACTG AATATGATGG GGCTGTATGA TGGGATGGAG
CACCTCATTT ATGGGGAGCC CAGGAAGCTG CTCACCCAAG ATTGGGTGCA GGAAAACTAC
CTGGAGTACC GGCAGGTGCC TGGCAGTGAT CCTGCACGGT ATGAGTTTCT GTGGGGTCCA
AGGGCTCATG CTGAAATTAG GAAGATGAGT CTCCTGAAAT TTTTGGCCAA GGTAAATGGG
AGTGATCCAA GATCCTTCCC ACTGTGGTAT GAGGAGGCTT TGAAAGATGA GGAAGAGAGA
GCCCAGGACA GAATTGCCAC CACAGATGAT ACTACTGCCA TGGCCAGTGC AAGTTCTAGC
GCTACAGGTA GCTTCTCCTA CCCTGAATAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.