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MAGEA12 melanoma antigen family A12 [Homo sapiens (human)]

Gene Symbol MAGEA12
Entrez Gene ID 4111
Full Name melanoma antigen family A12
Synonyms CT1.12, MAGE12
General protein information
Preferred Names
melanoma-associated antigen 12
Names
melanoma-associated antigen 12
MAGE-12 antigen
MAGE12F antigen
cancer/testis antigen 1.12
cancer/testis antigen family 1, member 12
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xq28

Summary This gene is closely related to several other genes clustered on chromosome X. These genes may be overexpressed in tumors. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq, Jun 2014]. lac of sum
Disorder MIM:

300177

Disorder Html:
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu27949 NM_001166386 Homo sapiens melanoma antigen family A12 (MAGEA12), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu27949 NM_001166387 Homo sapiens melanoma antigen family A12 (MAGEA12), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu27949 NM_005367 Homo sapiens melanoma antigen family A12 (MAGEA12), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu27949D
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 08-FEB-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 12
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC244102.3. On Apr 5, 2014 this sequence version replaced gi:594140643. Summary: This gene is closely related to several other genes clustered on chromosome X. These genes may be overexpressed in tumors. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq, Jun 2014]. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 all encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314168.1, BI861455.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCACTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA GGCCCAAGGA 
GAGGCCCTGG GCTTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GACTGCCTCC
TCCTCCTCTA CTCTAGTGGA AGTCACCCTG CGGGAGGTGC CTGCTGCCGA GTCACCAAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCACC CTCCCCACTA CCATCAACTA TACTCTCTGG
AGTCAATCCG ATGAGGGCTC CAGCAACGAA GAACAGGAAG GGCCAAGCAC CTTTCCTGAC
CTGGAGACGA GCTTCCAAGT AGCACTCAGT AGGAAGATGG CTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCATTC ACAAAGGCAG AAATGCTGGG GAGTGTCATC
AGAAATTTCC AGGACTTCTT TCCTGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CGCATCGGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGATCGTGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AAAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTGGAGGCA TCTGATGGGA GGGAGGACAG TGTCTTTGCG
CATCCCAGGA AGCTGCTCAC CCAAGATTTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATTTG CTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAATG GGCTTTTAGA GAGGGGGAAG AGTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001159858.1
CDS355..1299
Misc Feature(1)310..312(+)
Misc Feature(2)361..648(+)
Misc Feature(3)700..1212(+)
Exon (1)1..173
Gene:MAGEA12
Gene Synonym:
Exon (2)174..279
Gene:MAGEA12
Gene Synonym:
Exon (3)280..1862
Gene:MAGEA12
Gene Synonym:
Translation
Position Chain Variation Link
5 5 c, t dbSNP:3020648
78 78 a, g dbSNP:2515675
116 116 c, g dbSNP:73639100
173 173 c, t dbSNP:3020649
602 602 c, t dbSNP:267606388
622 622 a, g dbSNP:199979249
935 935 a, g dbSNP:112975238
966 966 c, t dbSNP:267606387
1051 1051 g, t dbSNP:141154162
1137 1137 c, g dbSNP:76420840
1242 1242 a, g dbSNP:2233055
1337 1337 a, g dbSNP:2233056
1349 1349 c, t dbSNP:113255279
1366 1366 c, t dbSNP:3005
1395 1395 c, t dbSNP:2515830
1588 1588 a, g dbSNP:111344124
1677 1677 a, g dbSNP:73629840
1800 1800 a, g dbSNP:2471773

Target ORF information:

RefSeq Version NM_001166386
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family A12 (MAGEA12), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001166386

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCACTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA GGCCCAAGGA 
GAGGCCCTGG GCTTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GACTGCCTCC
TCCTCCTCTA CTCTAGTGGA AGTCACCCTG CGGGAGGTGC CTGCTGCCGA GTCACCAAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCACC CTCCCCACTA CCATCAACTA TACTCTCTGG
AGTCAATCCG ATGAGGGCTC CAGCAACGAA GAACAGGAAG GGCCAAGCAC CTTTCCTGAC
CTGGAGACGA GCTTCCAAGT AGCACTCAGT AGGAAGATGG CTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCATTC ACAAAGGCAG AAATGCTGGG GAGTGTCATC
AGAAATTTCC AGGACTTCTT TCCTGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CGCATCGGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGATCGTGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AAAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTGGAGGCA TCTGATGGGA GGGAGGACAG TGTCTTTGCG
CATCCCAGGA AGCTGCTCAC CCAAGATTTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATTTG CTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAATG GGCTTTTAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu27949D
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 12
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC244102.3. On Apr 6, 2014 this sequence version replaced gi:594140683. Summary: This gene is closely related to several other genes clustered on chromosome X. These genes may be overexpressed in tumors. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq, Jun 2014]. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 all encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC003408.2, BT007108.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCACTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA GGCCCAAGGA 
GAGGCCCTGG GCTTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GACTGCCTCC
TCCTCCTCTA CTCTAGTGGA AGTCACCCTG CGGGAGGTGC CTGCTGCCGA GTCACCAAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCACC CTCCCCACTA CCATCAACTA TACTCTCTGG
AGTCAATCCG ATGAGGGCTC CAGCAACGAA GAACAGGAAG GGCCAAGCAC CTTTCCTGAC
CTGGAGACGA GCTTCCAAGT AGCACTCAGT AGGAAGATGG CTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCATTC ACAAAGGCAG AAATGCTGGG GAGTGTCATC
AGAAATTTCC AGGACTTCTT TCCTGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CGCATCGGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGATCGTGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AAAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTGGAGGCA TCTGATGGGA GGGAGGACAG TGTCTTTGCG
CATCCCAGGA AGCTGCTCAC CCAAGATTTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATTTG CTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAATG GGCTTTTAGA GAGGGGGAAG AGTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001159859.1
CDS263..1207
Misc Feature(1)218..220(+)
Misc Feature(2)269..556(+)
Misc Feature(3)608..1120(+)
Exon (1)1..81
Gene:MAGEA12
Gene Synonym:
Exon (2)82..187
Gene:MAGEA12
Gene Synonym:
Exon (3)188..1770
Gene:MAGEA12
Gene Synonym:
Translation
Position Chain Variation Link
45 45 a, c, t dbSNP:2515836
59 59 c, t dbSNP:12014569
510 510 c, t dbSNP:267606388
530 530 a, g dbSNP:199979249
843 843 a, g dbSNP:112975238
874 874 c, t dbSNP:267606387
959 959 g, t dbSNP:141154162
1045 1045 c, g dbSNP:76420840
1150 1150 a, g dbSNP:2233055
1245 1245 a, g dbSNP:2233056
1257 1257 c, t dbSNP:113255279
1274 1274 c, t dbSNP:3005
1303 1303 c, t dbSNP:2515830
1496 1496 a, g dbSNP:111344124
1585 1585 a, g dbSNP:73629840
1708 1708 a, g dbSNP:2471773

Target ORF information:

RefSeq Version NM_001166387
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family A12 (MAGEA12), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001166387

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCACTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA GGCCCAAGGA 
GAGGCCCTGG GCTTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GACTGCCTCC
TCCTCCTCTA CTCTAGTGGA AGTCACCCTG CGGGAGGTGC CTGCTGCCGA GTCACCAAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCACC CTCCCCACTA CCATCAACTA TACTCTCTGG
AGTCAATCCG ATGAGGGCTC CAGCAACGAA GAACAGGAAG GGCCAAGCAC CTTTCCTGAC
CTGGAGACGA GCTTCCAAGT AGCACTCAGT AGGAAGATGG CTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCATTC ACAAAGGCAG AAATGCTGGG GAGTGTCATC
AGAAATTTCC AGGACTTCTT TCCTGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CGCATCGGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGATCGTGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AAAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTGGAGGCA TCTGATGGGA GGGAGGACAG TGTCTTTGCG
CATCCCAGGA AGCTGCTCAC CCAAGATTTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATTTG CTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAATG GGCTTTTAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu27949D
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 12
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC244102.3. This sequence is a reference standard in the RefSeqGene project. On Apr 5, 2014 this sequence version replaced gi:261878479. Summary: This gene is closely related to several other genes clustered on chromosome X. These genes may be overexpressed in tumors. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq, Jun 2014]. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 all encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003408.2, BX341218.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCACTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA GGCCCAAGGA 
GAGGCCCTGG GCTTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GACTGCCTCC
TCCTCCTCTA CTCTAGTGGA AGTCACCCTG CGGGAGGTGC CTGCTGCCGA GTCACCAAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCACC CTCCCCACTA CCATCAACTA TACTCTCTGG
AGTCAATCCG ATGAGGGCTC CAGCAACGAA GAACAGGAAG GGCCAAGCAC CTTTCCTGAC
CTGGAGACGA GCTTCCAAGT AGCACTCAGT AGGAAGATGG CTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCATTC ACAAAGGCAG AAATGCTGGG GAGTGTCATC
AGAAATTTCC AGGACTTCTT TCCTGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CGCATCGGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGATCGTGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AAAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTGGAGGCA TCTGATGGGA GGGAGGACAG TGTCTTTGCG
CATCCCAGGA AGCTGCTCAC CCAAGATTTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATTTG CTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAATG GGCTTTTAGA GAGGGGGAAG AGTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_005358.2
CDS157..1101
Misc Feature(1)112..114(+)
Misc Feature(2)163..450(+)
Misc Feature(3)502..1014(+)
Exon (1)1..81
Gene:MAGEA12
Gene Synonym:
Exon (2)82..1664
Gene:MAGEA12
Gene Synonym:
Translation
Position Chain Variation Link
45 45 a, c, t dbSNP:2515836
59 59 c, t dbSNP:12014569
404 404 c, t dbSNP:267606388
424 424 a, g dbSNP:199979249
737 737 a, g dbSNP:112975238
768 768 c, t dbSNP:267606387
853 853 g, t dbSNP:141154162
939 939 c, g dbSNP:76420840
1044 1044 a, g dbSNP:2233055
1139 1139 a, g dbSNP:2233056
1151 1151 c, t dbSNP:113255279
1168 1168 c, t dbSNP:3005
1197 1197 c, t dbSNP:2515830
1390 1390 a, g dbSNP:111344124
1479 1479 a, g dbSNP:73629840
1602 1602 a, g dbSNP:2471773

Target ORF information:

RefSeq Version NM_005367
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family A12 (MAGEA12), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_005367

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCACTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA GGCCCAAGGA 
GAGGCCCTGG GCTTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GACTGCCTCC
TCCTCCTCTA CTCTAGTGGA AGTCACCCTG CGGGAGGTGC CTGCTGCCGA GTCACCAAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCACC CTCCCCACTA CCATCAACTA TACTCTCTGG
AGTCAATCCG ATGAGGGCTC CAGCAACGAA GAACAGGAAG GGCCAAGCAC CTTTCCTGAC
CTGGAGACGA GCTTCCAAGT AGCACTCAGT AGGAAGATGG CTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCATTC ACAAAGGCAG AAATGCTGGG GAGTGTCATC
AGAAATTTCC AGGACTTCTT TCCTGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CGCATCGGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGATCGTGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AAAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTGGAGGCA TCTGATGGGA GGGAGGACAG TGTCTTTGCG
CATCCCAGGA AGCTGCTCAC CCAAGATTTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATTTG CTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAATG GGCTTTTAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.