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MAGEB2 melanoma antigen family B2 [Homo sapiens (human)]

Gene Symbol MAGEB2
Entrez Gene ID 4113
Full Name melanoma antigen family B2
Synonyms CT3.2, DAM6, MAGE-XP-2
General protein information
Preferred Names
melanoma-associated antigen B2
Names
melanoma-associated antigen B2
MAGE-B2 antigen
MAGE XP-2 antigen
cancer/testis antigen 3.2
cancer/testis antigen family 3, member 2
DSS-AHC critical interval MAGE superfamily 6
DSS/AHC critical interval MAGE superfamily 6
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xp21.3

Summary This gene is a member of the MAGEB gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 68% sequence identity to each other. The promoters and first exons of the MAGEB genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. This gene is localized in the DSS (dosage-sensitive sex reversal) critical region. It is expressed in testis and placenta, and in a significant fraction of tumors of various histological types. The MAGEB genes are clustered on chromosome Xp22-p21. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

300098

Disorder Html:





The following MAGEB2 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGEB2 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu22525 XM_011545512 PREDICTED: Homo sapiens melanoma antigen family B2 (MAGEB2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu22525 NM_002364 Homo sapiens melanoma antigen family B2 (MAGEB2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $309

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu22525
Accession Version XM_011545512.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 960bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen B2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167197.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCGTG GTCAGAAGAG TAAGCTCCGT GCCCGTGAGA AACGCCGCAA GGCCCGAGAT 
GAGACCCGGG GTCTCAATGT TCCTCAGGTC ACTGAAGCAG AGGAAGAAGA GGCCCCCTGC
TGTTCCTCTT CTGTTTCTGG GGGTGCTGCT TCAAGCTCTC CTGCTGCTGG CATTCCCCAG
GAGCCTCAGA GAGCCCCAAC CACTGCCGCT GCTGCGGCTG CGGGTGTTTC ATCCACAAAA
TCTAAAAAAG GTGCCAAGAG CCACCAAGGT GAGAAAAATG CAAGTTCCTC CCAGGCCTCA
ACATCCACTA AGAGCCCAAG CGAAGATCCT CTAACCAGGA AGTCAGGGTC GTTGGTGCAG
TTCCTGTTGT ACAAGTATAA AATAAAAAAG TCCGTTACAA AGGGAGAAAT GCTGAAAATT
GTTGGCAAAA GGTTCAGGGA GCACTTCCCT GAGATCCTCA AGAAAGCCTC TGAGGGCCTC
AGTGTTGTCT TTGGCCTTGA GCTGAATAAA GTCAACCCCA ACGGCCACAC TTACACCTTC
ATCGACAAGG TAGACCTCAC TGATGAGGAA TCCCTGCTCA GTTCCTGGGA CTTTCCCAGG
AGAAAGCTTC TGATGCCTCT CCTGGGTGTG ATCTTCTTAA ATGGCAACTC AGCTACTGAG
GAAGAGATCT GGGAATTCCT GAATATGTTG GGAGTCTATG ATGGAGAGGA GCACTCAGTC
TTTGGGGAAC CCTGGAAGCT CATCACCAAA GATCTGGTGC AGGAAAAATA TCTGGAGTAC
AAGCAGGTGC CCAGCAGTGA TCCCCCACGC TTTCAATTCC TGTGGGGTCC GAGAGCCTAT
GCTGAAACCA GCAAGATGAA AGTCCTGGAG TTTTTGGCCA AGGTAAATGG TACCACCCCC
TGTGCCTTCC CAACCCATTA CGAAGAAGCT TTGAAAGATG AAGAGAAAGC CGGAGTCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011543814.1
CDS144..1103
Misc Feature(1)150..419(+)
Misc Feature(2)495..1007(+)
Translation
Position Chain Variation Link
12 12 a, g dbSNP:190886045
19 19 a, g dbSNP:367778841
22 22 c, t dbSNP:756683025
27 27 a, c dbSNP:780526167
42 42 c, t dbSNP:770424280
82 82 a, g dbSNP:748798560
93 93 c, t dbSNP:777521213
97 97 c, t dbSNP:770873217
104 104 c, t dbSNP:6526962
108 108 -, ttc dbSNP:778411044
115 115 a, g dbSNP:780267669
119 119 a, t dbSNP:747116536
124 124 g, t dbSNP:368971229
125 125 c, t dbSNP:777044216
134 134 -, c dbSNP:751044054
135 135 c, t dbSNP:200333593
139 139 c, g dbSNP:762392461
143 143 c, g dbSNP:770379532
144 144 a, g dbSNP:750718375
147 147 c, t dbSNP:763345977
150 150 c, t dbSNP:765958802
151 151 a, g dbSNP:746022879
157 157 a, g dbSNP:759031616
167 167 g, t dbSNP:767023113
170 170 c, t dbSNP:752680338
172 172 a, g dbSNP:756066149
174 174 g, t dbSNP:777645955
177 177 a, c, t dbSNP:373920316
178 178 a, g dbSNP:376868079
186 186 c, t dbSNP:747224013
187 187 a, g dbSNP:768671683
189 189 c, t dbSNP:200649828
190 190 a, g, t dbSNP:151181148
198 198 c, t dbSNP:773794633
199 199 a, g dbSNP:199746320
210 210 c, g, t dbSNP:369826731
213 213 g, t dbSNP:759205287
220 220 a, g dbSNP:139121250
245 245 a, g dbSNP:775011252
248 248 a, g dbSNP:370758512
257 257 c, t dbSNP:375229915
259 259 a, c dbSNP:149932758
260 260 c, g dbSNP:755444019
261 261 a, t dbSNP:757043954
265 265 a, g, t dbSNP:74852235
267 267 c, t dbSNP:755263954
270 270 g, t dbSNP:766889118
271 271 a, c dbSNP:748255078
303 303 -, ctg dbSNP:747474804
306 306 c, g dbSNP:756246520
310 310 c, t dbSNP:778488431
315 315 a, t dbSNP:749778121
318 318 c, t dbSNP:771249184
324 324 a, g dbSNP:2529541
330 330 c, t dbSNP:746176783
333 333 a, g dbSNP:771758637
349 349 a, c dbSNP:775111636
350 350 c, t dbSNP:370067105
351 351 a, g dbSNP:183272477
355 355 a, c dbSNP:776599379
357 357 -, gcggc dbSNP:771206196
358 358 c, t dbSNP:761837486
359 359 a, g dbSNP:5973792
363 363 -, gcggg dbSNP:776998052
364 364 c, t dbSNP:750240402
365 365 a, g dbSNP:200872475
368 368 g, t dbSNP:767906444
369 369 a, g dbSNP:752960894
373 373 c, t dbSNP:756299317
389 389 a, c dbSNP:754734083
403 403 a, g dbSNP:146783301
422 422 c, g, t dbSNP:148939834
426 426 a, g dbSNP:368665231
449 449 ca, tg dbSNP:386824543
449 449 c, t dbSNP:2074793
450 450 a, g dbSNP:7878409
463 463 a, g dbSNP:779723991
464 464 c, t dbSNP:757368435
465 465 a, g dbSNP:368561784
470 470 c, t dbSNP:141924015
474 474 c, t dbSNP:768249275
479 479 c, t dbSNP:781070614
485 485 g, t dbSNP:761165706
489 489 c, g dbSNP:745960048
492 492 c, t dbSNP:773013541
498 498 a, g dbSNP:370817698
510 510 c, t dbSNP:762869845
513 513 c, t dbSNP:189110298
517 517 a, g dbSNP:753012331
518 518 g, t dbSNP:760996564
522 522 a, g dbSNP:374368116
525 525 -, tat dbSNP:746102460
527 527 a, g, t dbSNP:150250737
529 529 a, t dbSNP:779542719
536 536 c, t dbSNP:139272287
537 537 a, g dbSNP:758812052
543 543 a, g dbSNP:780419769
553 553 c, t dbSNP:747338040
555 555 c, g dbSNP:768306363
561 561 a, g dbSNP:143298503
562 562 c, t dbSNP:769451362
575 575 c, g dbSNP:368299444
584 584 a, g dbSNP:371753005
611 611 c, g dbSNP:747754701
613 613 c, t dbSNP:769180547
621 621 c, t dbSNP:773239129
642 642 c, g dbSNP:147546492
644 644 a, g dbSNP:375117732
646 646 c, t dbSNP:774317963
649 649 a, g dbSNP:759337831
661 661 c, t dbSNP:764422051
662 662 c, t dbSNP:753991418
663 663 a, g dbSNP:193132352
664 664 a, g dbSNP:765211660
665 665 c, t dbSNP:750467118
666 666 a, g, t dbSNP:758936510
679 679 c, t dbSNP:751938258
686 686 c, g, t dbSNP:140291899
687 687 a, g dbSNP:199666309
714 714 a, t dbSNP:755696118
722 722 c, t dbSNP:777166552
730 730 c, g dbSNP:748764119
731 731 a, g dbSNP:770996632
732 732 c, g dbSNP:184471774
738 738 a, c dbSNP:45513291
746 746 a, g dbSNP:771735549
760 760 c, t dbSNP:776963214
767 767 a, g dbSNP:761964672
769 769 a, g dbSNP:765460965
775 775 -, tct dbSNP:780694497
790 790 a, c, g dbSNP:149245405
795 795 a, g dbSNP:367729095
796 796 c, g dbSNP:752059348
798 798 -, a dbSNP:775590244
798 798 a, g dbSNP:755372967
814 814 c, g dbSNP:767747644
826 826 a, g dbSNP:774207887
839 839 a, c, t dbSNP:371294125
841 841 a, t dbSNP:777415745
858 858 c, t dbSNP:748819651
868 868 a, g dbSNP:756812571
874 874 c, t dbSNP:779059004
878 878 a, g dbSNP:745779246
881 881 g, t dbSNP:771999721
882 882 c, t dbSNP:376780034
893 893 a, t dbSNP:200726761
896 896 c, t dbSNP:370019799
897 897 c, g dbSNP:769925797
914 914 c, t dbSNP:144472411
916 916 -, tg dbSNP:772060099
922 922 a, g dbSNP:763090072
926 926 a, g dbSNP:200975893
933 933 a, c dbSNP:767384723
946 946 c, g dbSNP:766418758
951 951 c, t dbSNP:774884656
952 952 c, g dbSNP:759980804
959 959 a, g dbSNP:768025185
963 963 a, c, t dbSNP:148432603
970 970 a, g dbSNP:772656322
973 973 c, t dbSNP:142602767
974 974 a, g dbSNP:760918442
982 982 a, g dbSNP:146480900
987 987 a, g dbSNP:756877832
993 993 a, g dbSNP:778590723
999 999 a, g, t dbSNP:760208597
1001 1001 c, g dbSNP:779986988
1006 1006 c, t dbSNP:766710849
1010 1010 a, g dbSNP:768495623
1012 1012 a, g dbSNP:374398423
1015 1015 g, t dbSNP:377141601
1035 1035 a, g dbSNP:771089577
1036 1036 a, c dbSNP:374280154
1037 1037 c, t dbSNP:759589923
1042 1042 c, g dbSNP:772469237
1044 1044 -, t dbSNP:773023961
1054 1054 c, g dbSNP:776050887
1055 1055 a, t dbSNP:200351837
1064 1064 c, t dbSNP:764521778
1065 1065 -, g dbSNP:765406264
1068 1068 g, t dbSNP:754207788
1071 1071 a, g dbSNP:369559506
1094 1094 c, t dbSNP:139938143
1095 1095 a, g dbSNP:5972090
1107 1107 a, g dbSNP:757914942
1116 1116 a, g dbSNP:373793135
1119 1119 a, g dbSNP:751488182
1129 1129 c, t dbSNP:754781859
1132 1132 c, g dbSNP:780983841
1141 1141 c, t dbSNP:747893990
1144 1144 a, g dbSNP:376379710
1145 1145 c, t dbSNP:143715628
1148 1148 a, g dbSNP:202101229
1187 1187 a, g dbSNP:753184302
1248 1248 c, t dbSNP:752688181
1256 1256 g, t dbSNP:758339197
1270 1270 -, t dbSNP:777755383
1271 1271 -, t dbSNP:538142406
1271 1271 -, t dbSNP:368622211
1280 1280 c, t dbSNP:78970058
1281 1281 -, t dbSNP:147123518
1283 1283 c, t dbSNP:3180333
1306 1306 a, t dbSNP:756232146
1315 1315 g, t dbSNP:758934787
1317 1317 g, t dbSNP:542465117
1327 1327 a, g dbSNP:147147385
1328 1328 -, a dbSNP:769156703
1347 1347 a, g dbSNP:187749937
1386 1386 c, g dbSNP:557461332
1433 1433 c, t dbSNP:778053965
1434 1434 a, c dbSNP:748531899
1435 1435 a, t dbSNP:7056939
1465 1465 c, t dbSNP:773736404
1466 1466 a, g dbSNP:760131792
1472 1472 a, g dbSNP:5972091
1478 1478 a, g dbSNP:779742426
1508 1508 c, t dbSNP:748811886
1536 1536 g, t dbSNP:1053918
1565 1565 a, c dbSNP:191526571
1642 1642 -, tagt dbSNP:764868986

Target ORF information:

RefSeq Version XM_011545512
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens melanoma antigen family B2 (MAGEB2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011545512

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCGTG GTCAGAAGAG TAAGCTCCGT GCCCGTGAGA AACGCCGCAA GGCCCGAGAT 
GAGACCCGGG GTCTCAATGT TCCTCAGGTC ACTGAAGCAG AGGAAGAAGA GGCCCCCTGC
TGTTCCTCTT CTGTTTCTGG GGGTGCTGCT TCAAGCTCTC CTGCTGCTGG CATTCCCCAG
GAGCCTCAGA GAGCCCCAAC CACTGCCGCT GCTGCGGCTG CGGGTGTTTC ATCCACAAAA
TCTAAAAAAG GTGCCAAGAG CCACCAAGGT GAGAAAAATG CAAGTTCCTC CCAGGCCTCA
ACATCCACTA AGAGCCCAAG CGAAGATCCT CTAACCAGGA AGTCAGGGTC GTTGGTGCAG
TTCCTGTTGT ACAAGTATAA AATAAAAAAG TCCGTTACAA AGGGAGAAAT GCTGAAAATT
GTTGGCAAAA GGTTCAGGGA GCACTTCCCT GAGATCCTCA AGAAAGCCTC TGAGGGCCTC
AGTGTTGTCT TTGGCCTTGA GCTGAATAAA GTCAACCCCA ACGGCCACAC TTACACCTTC
ATCGACAAGG TAGACCTCAC TGATGAGGAA TCCCTGCTCA GTTCCTGGGA CTTTCCCAGG
AGAAAGCTTC TGATGCCTCT CCTGGGTGTG ATCTTCTTAA ATGGCAACTC AGCTACTGAG
GAAGAGATCT GGGAATTCCT GAATATGTTG GGAGTCTATG ATGGAGAGGA GCACTCAGTC
TTTGGGGAAC CCTGGAAGCT CATCACCAAA GATCTGGTGC AGGAAAAATA TCTGGAGTAC
AAGCAGGTGC CCAGCAGTGA TCCCCCACGC TTTCAATTCC TGTGGGGTCC GAGAGCCTAT
GCTGAAACCA GCAAGATGAA AGTCCTGGAG TTTTTGGCCA AGGTAAATGG TACCACCCCC
TGTGCCTTCC CAACCCATTA CGAAGAAGCT TTGAAAGATG AAGAGAAAGC CGGAGTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22525
Accession Version NM_002364.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 960bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen B2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005185.1 and AK223081.1. This sequence is a reference standard in the RefSeqGene project. On or before Jul 8, 2009 this sequence version replaced gi:239757771, gi:34335239. Summary: This gene is a member of the MAGEB gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 68% sequence identity to each other. The promoters and first exons of the MAGEB genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. This gene is localized in the DSS (dosage-sensitive sex reversal) critical region. It is expressed in testis and placenta, and in a significant fraction of tumors of various histological types. The MAGEB genes are clustered on chromosome Xp22-p21. [provided by RefSeq, Jul 2008]. ##Evidence-Data-START## Transcript exon combination :: BC026071.2, AF015766.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145122 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCGTG GTCAGAAGAG TAAGCTCCGT GCCCGTGAGA AACGCCGCAA GGCCCGAGAT 
GAGACCCGGG GTCTCAATGT TCCTCAGGTC ACTGAAGCAG AGGAAGAAGA GGCCCCCTGC
TGTTCCTCTT CTGTTTCTGG GGGTGCTGCT TCAAGCTCTC CTGCTGCTGG CATTCCCCAG
GAGCCTCAGA GAGCCCCAAC CACTGCCGCT GCTGCGGCTG CGGGTGTTTC ATCCACAAAA
TCTAAAAAAG GTGCCAAGAG CCACCAAGGT GAGAAAAATG CAAGTTCCTC CCAGGCCTCA
ACATCCACTA AGAGCCCAAG CGAAGATCCT CTAACCAGGA AGTCAGGGTC GTTGGTGCAG
TTCCTGTTGT ACAAGTATAA AATAAAAAAG TCCGTTACAA AGGGAGAAAT GCTGAAAATT
GTTGGCAAAA GGTTCAGGGA GCACTTCCCT GAGATCCTCA AGAAAGCCTC TGAGGGCCTC
AGTGTTGTCT TTGGCCTTGA GCTGAATAAA GTCAACCCCA ACGGCCACAC TTACACCTTC
ATCGACAAGG TAGACCTCAC TGATGAGGAA TCCCTGCTCA GTTCCTGGGA CTTTCCCAGG
AGAAAGCTTC TGATGCCTCT CCTGGGTGTG ATCTTCTTAA ATGGCAACTC AGCTACTGAG
GAAGAGATCT GGGAATTCCT GAATATGTTG GGAGTCTATG ATGGAGAGGA GCACTCAGTC
TTTGGGGAAC CCTGGAAGCT CATCACCAAA GATCTGGTGC AGGAAAAATA TCTGGAGTAC
AAGCAGGTGC CCAGCAGTGA TCCCCCACGC TTTCAATTCC TGTGGGGTCC GAGAGCCTAT
GCTGAAACCA GCAAGATGAA AGTCCTGGAG TTTTTGGCCA AGGTAAATGG TACCACCCCC
TGTGCCTTCC CAACCCATTA CGAAGAAGCT TTGAAAGATG AAGAGAAAGC CGGAGTCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_002355.2
CDS104..1063
Misc Feature(1)110..379(+)
Misc Feature(2)332..334(+)
Misc Feature(3)416..418(+)
Misc Feature(4)455..967(+)
Exon (1)1..98
Gene:MAGEB2
Gene Synonym:
Exon (2)99..1612
Gene:MAGEB2
Gene Synonym:
Translation
Position Chain Variation Link
7 7 c, t dbSNP:757443638
19 19 a, g dbSNP:190886045
26 26 a, g dbSNP:367778841
29 29 c, t dbSNP:756683025
34 34 a, c dbSNP:780526167
49 49 c, t dbSNP:770424280
89 89 a, g dbSNP:748798560
99 99 c, g dbSNP:762392461
103 103 c, g dbSNP:770379532
104 104 a, g dbSNP:750718375
107 107 c, t dbSNP:763345977
110 110 c, t dbSNP:765958802
111 111 a, g dbSNP:746022879
117 117 a, g dbSNP:759031616
127 127 g, t dbSNP:767023113
130 130 c, t dbSNP:752680338
132 132 a, g dbSNP:756066149
134 134 g, t dbSNP:777645955
137 137 a, c, t dbSNP:373920316
138 138 a, g dbSNP:376868079
146 146 c, t dbSNP:747224013
147 147 a, g dbSNP:768671683
149 149 c, t dbSNP:200649828
150 150 a, g, t dbSNP:151181148
158 158 c, t dbSNP:773794633
159 159 a, g dbSNP:199746320
170 170 c, g, t dbSNP:369826731
173 173 g, t dbSNP:759205287
180 180 a, g dbSNP:139121250
205 205 a, g dbSNP:775011252
208 208 a, g dbSNP:370758512
217 217 c, t dbSNP:375229915
219 219 a, c dbSNP:149932758
220 220 c, g dbSNP:755444019
221 221 a, t dbSNP:757043954
225 225 a, g, t dbSNP:74852235
227 227 c, t dbSNP:755263954
230 230 g, t dbSNP:766889118
231 231 a, c dbSNP:748255078
263 263 -, ctg dbSNP:747474804
266 266 c, g dbSNP:756246520
270 270 c, t dbSNP:778488431
275 275 a, t dbSNP:749778121
278 278 c, t dbSNP:771249184
284 284 a, g dbSNP:2529541
290 290 c, t dbSNP:746176783
293 293 a, g dbSNP:771758637
309 309 a, c dbSNP:775111636
310 310 c, t dbSNP:370067105
311 311 a, g dbSNP:183272477
315 315 a, c dbSNP:776599379
317 317 -, gcggc dbSNP:771206196
318 318 c, t dbSNP:761837486
319 319 a, g dbSNP:5973792
323 323 -, gcggg dbSNP:776998052
324 324 c, t dbSNP:750240402
325 325 a, g dbSNP:200872475
328 328 g, t dbSNP:767906444
329 329 a, g dbSNP:752960894
333 333 c, t dbSNP:756299317
349 349 a, c dbSNP:754734083
363 363 a, g dbSNP:146783301
382 382 c, g, t dbSNP:148939834
386 386 a, g dbSNP:368665231
409 409 ca, tg dbSNP:386824543
409 409 c, t dbSNP:2074793
410 410 a, g dbSNP:7878409
423 423 a, g dbSNP:779723991
424 424 c, t dbSNP:757368435
425 425 a, g dbSNP:368561784
430 430 c, t dbSNP:141924015
434 434 c, t dbSNP:768249275
439 439 c, t dbSNP:781070614
445 445 g, t dbSNP:761165706
449 449 c, g dbSNP:745960048
452 452 c, t dbSNP:773013541
458 458 a, g dbSNP:370817698
470 470 c, t dbSNP:762869845
473 473 c, t dbSNP:189110298
477 477 a, g dbSNP:753012331
478 478 g, t dbSNP:760996564
482 482 a, g dbSNP:374368116
485 485 -, tat dbSNP:746102460
487 487 a, g, t dbSNP:150250737
489 489 a, t dbSNP:779542719
496 496 c, t dbSNP:139272287
497 497 a, g dbSNP:758812052
503 503 a, g dbSNP:780419769
513 513 c, t dbSNP:747338040
515 515 c, g dbSNP:768306363
521 521 a, g dbSNP:143298503
522 522 c, t dbSNP:769451362
535 535 c, g dbSNP:368299444
544 544 a, g dbSNP:371753005
571 571 c, g dbSNP:747754701
573 573 c, t dbSNP:769180547
581 581 c, t dbSNP:773239129
602 602 c, g dbSNP:147546492
604 604 a, g dbSNP:375117732
606 606 c, t dbSNP:774317963
609 609 a, g dbSNP:759337831
621 621 c, t dbSNP:764422051
622 622 c, t dbSNP:753991418
623 623 a, g dbSNP:193132352
624 624 a, g dbSNP:765211660
625 625 c, t dbSNP:750467118
626 626 a, g, t dbSNP:758936510
639 639 c, t dbSNP:751938258
646 646 c, g, t dbSNP:140291899
647 647 a, g dbSNP:199666309
674 674 a, t dbSNP:755696118
682 682 c, t dbSNP:777166552
690 690 c, g dbSNP:748764119
691 691 a, g dbSNP:770996632
692 692 c, g dbSNP:184471774
698 698 a, c dbSNP:45513291
706 706 a, g dbSNP:771735549
720 720 c, t dbSNP:776963214
727 727 a, g dbSNP:761964672
729 729 a, g dbSNP:765460965
735 735 -, tct dbSNP:780694497
750 750 a, c, g dbSNP:149245405
755 755 a, g dbSNP:367729095
756 756 c, g dbSNP:752059348
758 758 -, a dbSNP:775590244
758 758 a, g dbSNP:755372967
774 774 c, g dbSNP:767747644
786 786 a, g dbSNP:774207887
799 799 a, c, t dbSNP:371294125
801 801 a, t dbSNP:777415745
818 818 c, t dbSNP:748819651
828 828 a, g dbSNP:756812571
834 834 c, t dbSNP:779059004
838 838 a, g dbSNP:745779246
841 841 g, t dbSNP:771999721
842 842 c, t dbSNP:376780034
853 853 a, t dbSNP:200726761
856 856 c, t dbSNP:370019799
857 857 c, g dbSNP:769925797
874 874 c, t dbSNP:144472411
876 876 -, tg dbSNP:772060099
882 882 a, g dbSNP:763090072
886 886 a, g dbSNP:200975893
893 893 a, c dbSNP:767384723
906 906 c, g dbSNP:766418758
911 911 c, t dbSNP:774884656
912 912 c, g dbSNP:759980804
919 919 a, g dbSNP:768025185
923 923 a, c, t dbSNP:148432603
930 930 a, g dbSNP:772656322
933 933 c, t dbSNP:142602767
934 934 a, g dbSNP:760918442
942 942 a, g dbSNP:146480900
947 947 a, g dbSNP:756877832
953 953 a, g dbSNP:778590723
959 959 a, g, t dbSNP:760208597
961 961 c, g dbSNP:779986988
966 966 c, t dbSNP:766710849
970 970 a, g dbSNP:768495623
972 972 a, g dbSNP:374398423
975 975 g, t dbSNP:377141601
995 995 a, g dbSNP:771089577
996 996 a, c dbSNP:374280154
997 997 c, t dbSNP:759589923
1002 1002 c, g dbSNP:772469237
1004 1004 -, t dbSNP:773023961
1014 1014 c, g dbSNP:776050887
1015 1015 a, t dbSNP:200351837
1024 1024 c, t dbSNP:764521778
1025 1025 -, g dbSNP:765406264
1028 1028 g, t dbSNP:754207788
1031 1031 a, g dbSNP:369559506
1054 1054 c, t dbSNP:139938143
1055 1055 a, g dbSNP:5972090
1067 1067 a, g dbSNP:757914942
1076 1076 a, g dbSNP:373793135
1079 1079 a, g dbSNP:751488182
1089 1089 c, t dbSNP:754781859
1092 1092 c, g dbSNP:780983841
1101 1101 c, t dbSNP:747893990
1104 1104 a, g dbSNP:376379710
1105 1105 c, t dbSNP:143715628
1108 1108 a, g dbSNP:202101229
1147 1147 a, g dbSNP:753184302
1208 1208 c, t dbSNP:752688181
1216 1216 g, t dbSNP:758339197
1230 1230 -, t dbSNP:777755383
1231 1231 -, t dbSNP:538142406
1231 1231 -, t dbSNP:368622211
1240 1240 c, t dbSNP:78970058
1241 1241 -, t dbSNP:147123518
1243 1243 c, t dbSNP:3180333
1266 1266 a, t dbSNP:756232146
1275 1275 g, t dbSNP:758934787
1277 1277 g, t dbSNP:542465117
1287 1287 a, g dbSNP:147147385
1288 1288 -, a dbSNP:769156703
1307 1307 a, g dbSNP:187749937
1346 1346 c, g dbSNP:557461332
1393 1393 c, t dbSNP:778053965
1394 1394 a, c dbSNP:748531899
1395 1395 a, t dbSNP:7056939
1425 1425 c, t dbSNP:773736404
1426 1426 a, g dbSNP:760131792
1432 1432 a, g dbSNP:5972091
1438 1438 a, g dbSNP:779742426
1468 1468 c, t dbSNP:748811886
1496 1496 g, t dbSNP:1053918
1525 1525 a, c dbSNP:191526571
1602 1602 -, tagt dbSNP:764868986

Target ORF information:

RefSeq Version NM_002364
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family B2 (MAGEB2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002364

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCGTG GTCAGAAGAG TAAGCTCCGT GCCCGTGAGA AACGCCGCAA GGCCCGAGAT 
GAGACCCGGG GTCTCAATGT TCCTCAGGTC ACTGAAGCAG AGGAAGAAGA GGCCCCCTGC
TGTTCCTCTT CTGTTTCTGG GGGTGCTGCT TCAAGCTCTC CTGCTGCTGG CATTCCCCAG
GAGCCTCAGA GAGCCCCAAC CACTGCCGCT GCTGCGGCTG CGGGTGTTTC ATCCACAAAA
TCTAAAAAAG GTGCCAAGAG CCACCAAGGT GAGAAAAATG CAAGTTCCTC CCAGGCCTCA
ACATCCACTA AGAGCCCAAG CGAAGATCCT CTAACCAGGA AGTCAGGGTC GTTGGTGCAG
TTCCTGTTGT ACAAGTATAA AATAAAAAAG TCCGTTACAA AGGGAGAAAT GCTGAAAATT
GTTGGCAAAA GGTTCAGGGA GCACTTCCCT GAGATCCTCA AGAAAGCCTC TGAGGGCCTC
AGTGTTGTCT TTGGCCTTGA GCTGAATAAA GTCAACCCCA ACGGCCACAC TTACACCTTC
ATCGACAAGG TAGACCTCAC TGATGAGGAA TCCCTGCTCA GTTCCTGGGA CTTTCCCAGG
AGAAAGCTTC TGATGCCTCT CCTGGGTGTG ATCTTCTTAA ATGGCAACTC AGCTACTGAG
GAAGAGATCT GGGAATTCCT GAATATGTTG GGAGTCTATG ATGGAGAGGA GCACTCAGTC
TTTGGGGAAC CCTGGAAGCT CATCACCAAA GATCTGGTGC AGGAAAAATA TCTGGAGTAC
AAGCAGGTGC CCAGCAGTGA TCCCCCACGC TTTCAATTCC TGTGGGGTCC GAGAGCCTAT
GCTGAAACCA GCAAGATGAA AGTCCTGGAG TTTTTGGCCA AGGTAAATGG TACCACCCCC
TGTGCCTTCC CAACCCATTA CGAAGAAGCT TTGAAAGATG AAGAGAAAGC CGGAGTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.