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MAGEB3 melanoma antigen family B3 [Homo sapiens (human)]

Gene Symbol MAGEB3
Entrez Gene ID 4114
Full Name melanoma antigen family B3
Synonyms CT3.5
General protein information
Preferred Names
melanoma-associated antigen B3
Names
melanoma-associated antigen B3
MAGE-B3 antigen
cancer/testis antigen family 3, member 5
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xp21.3

Summary This gene is a MAGE-B subfamily member of the MAGE gene family. MAGE family member proteins direct the expression of tumor antigens recognized on a human melanoma by autologous cytolytic T lymphocytes. There are two known clusters of MAGE genes on chromosome X. The members of the MAGE-A subfamily are located in the Xq28 region, while the members of the MAGE-B subfamily are clustered in the Xp21 region. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

300152

Disorder Html:
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu17808 XM_011545513 PREDICTED: Homo sapiens melanoma antigen family B3 (MAGEB3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu17808 XM_011545514 PREDICTED: Homo sapiens melanoma antigen family B3 (MAGEB3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu17808 NM_002365 Homo sapiens melanoma antigen family B3 (MAGEB3), mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu17808D
Sequence Information ORF Nucleotide Sequence (Length: 1041bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen B3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167197.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGCCTCGGG GTCAGAAGAG TACGCTCCAT GCACGTGAGA AACGCCAGCA GACCCGGGGT 
CAGACCCAGG ATCACCAGGG TGCTCAGATC ACTGCAACTA ACAAGAAAAA AGTATCCTTT
TCATCCCCTC TTATTTTGGG GGCTACTATC CAGAAAAAGT CTGCTGGTAG GTCACGTAGT
GCTCTCAAGA AGCCTCAGAG AGCACTATCC ACCACTACAT CTGTAGATGT TTCTTACAAA
AAGTCATACA AGGGAGCCAA CAGCAAAATT GAGAAAAAGC AAAGCTTCTC TCAGGGTCTA
TCCTCCACTG TGCAGTCTCG CACAGACCCT CTAATCATGA AGACAAATAT GTTGGTGCAG
TTCCTGATGG AAATGTACAA GATGAAAAAG CCCATTATGA AAGCAGATAT GCTAAAAATT
GTCCAAAAAA GCCATAAGAA TTGCTTCCCT GAGATCCTTA AAAAAGCTTC TTTCAACATG
GAGGTGGTAT TTGGTGTTGA TTTAAAGAAA GTTGATTCTA CCAAGGACTC CTATGTCCTT
GTCAGCAAAA TGGATCTCCC CAACAATGGG ACAGTGACTC GTGGGAGGGG ATTTCCCAAG
ACAGGTCTCC TGCTGAATCT CCTGGGCGTG ATCTTCATGA AGGGCAACTG TGCCACTGAG
GAGAAGATCT GGGAATTCCT GAATAAGATG AGAATATATG ATGGGAAGAA ACACTTCATA
TTTGGGGAGC CCAGAAAGCT CATCACCCAA GATTTGGTGA AGCTTAAATA CCTGGAGTAC
CGACAAGTGC CCAACAGTAA TCCTGCACGC TATGAATTCC TGTGGGGTCC AAGAGCCCAT
GCTGAAACCA GCAAGATGAA GGTCCTGGAG TTTTGGGCCA AGGTCAATAA AACTGTCCCC
AGTGCGTTCC AGTTCTGGTA TGAAGAGGCT TTGAGAGATG AGGAAGAAAG AGTCCAAGCT
GCAGCTATGC TCAATGATGG CAGTAGTGCC ATGGGCAGAA AGTGTTCCAA GGCCAAGGCT
AGCAGCTCTT CCCACGCCTTA G
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011543815.1
CDS645..1685
Misc Feature(1)651..935(+)
Misc Feature(2)996..1508(+)
Translation
Position Chain Variation Link
145 145 a, t dbSNP:544432860
147 147 c, t dbSNP:752232097
167 167 a, g dbSNP:183421732
247 247 c, g dbSNP:777493378
276 276 a, g dbSNP:774420245
279 279 a, g dbSNP:751357659
311 311 a, g dbSNP:757191381
318 318 c, t dbSNP:138620085
346 346 c, g dbSNP:184916010
353 353 a, g dbSNP:768677107
400 400 c, g dbSNP:763685972
432 432 c, t dbSNP:191550484
447 447 c, t dbSNP:376392121
460 460 a, c dbSNP:775102866
464 464 a, g dbSNP:761614262
472 472 a, g dbSNP:62585752
490 490 c, g dbSNP:776027914
596 596 a, c dbSNP:772186001
598 598 a, t dbSNP:762704445
608 608 c, g dbSNP:761267560
612 612 c, t dbSNP:769246646
614 614 c, t dbSNP:777142499
617 617 c, t dbSNP:762212684
629 629 c, t dbSNP:765043330
632 632 a, g dbSNP:376932552
633 633 a, c dbSNP:371123599
636 636 a, g dbSNP:374516482
652 652 a, g dbSNP:146770977
654 654 a, g dbSNP:754935662
666 666 a, g dbSNP:764346553
667 667 c, t dbSNP:201131077
668 668 a, g dbSNP:752498887
677 677 a, g dbSNP:761018084
678 678 c, g dbSNP:766493179
687 687 c, t dbSNP:140421974
688 688 a, g dbSNP:370624672
700 700 a, g dbSNP:150405296
707 707 a, g dbSNP:746993866
714 714 g, t dbSNP:769301868
719 719 c, g dbSNP:777265461
736 736 c, t dbSNP:202246584
752 752 a, g dbSNP:770254826
770 770 c, t dbSNP:372226669
776 776 c, t dbSNP:201088914
780 780 c, t dbSNP:765904215
784 784 a, g dbSNP:773912188
793 793 c, t dbSNP:182812872
803 803 g, t dbSNP:767498329
804 804 c, t dbSNP:145229582
808 808 c, t dbSNP:755941263
814 814 a, g dbSNP:763926751
818 818 a, c dbSNP:753527600
819 819 c, t dbSNP:758651543
820 820 a, g dbSNP:780082636
837 837 c, g, t dbSNP:747172967
839 839 c, t dbSNP:781775253
851 851 a, g dbSNP:779403087
866 866 g, t dbSNP:770379507
867 867 a, g dbSNP:149158914
868 868 a, t dbSNP:749543764
881 881 c, t dbSNP:146621587
904 904 a, t dbSNP:750532535
913 913 c, t dbSNP:187371545
930 930 c, t dbSNP:756681502
933 933 c, t dbSNP:780480082
945 945 g, t dbSNP:766981324
946 946 a, c dbSNP:372021808
951 951 a, c dbSNP:777321413
954 954 a, g dbSNP:763872315
960 960 c, t dbSNP:139231962
963 963 c, g dbSNP:761502732
964 964 a, g dbSNP:2071308
972 972 c, t dbSNP:375048517
979 979 c, t dbSNP:2071309
995 995 a, g dbSNP:781448360
1010 1010 a, g dbSNP:752787599
1014 1014 c, g dbSNP:756645824
1018 1018 g, t dbSNP:778080700
1020 1020 c, t dbSNP:749717727
1021 1021 a, g dbSNP:771192209
1022 1022 c, t dbSNP:778666100
1027 1027 c, t dbSNP:745495531
1041 1041 a, g dbSNP:771663707
1042 1042 c, t dbSNP:775011357
1074 1074 a, g dbSNP:760122075
1077 1077 c, t dbSNP:768698062
1081 1081 a, g dbSNP:776429024
1093 1093 c, t dbSNP:761710997
1095 1095 a, g dbSNP:764921111
1104 1104 -, a dbSNP:34054171
1116 1116 g, t dbSNP:750198978
1130 1130 g, t dbSNP:759872010
1133 1133 a, g dbSNP:2071310
1135 1135 g, t dbSNP:752834151
1148 1148 -, aaag dbSNP:760599195
1154 1154 a, g dbSNP:769655784
1172 1172 c, t dbSNP:778408289
1178 1178 c, t dbSNP:754266826
1182 1182 a, c dbSNP:113117104
1185 1185 a, g dbSNP:757687731
1187 1187 a, c dbSNP:779272274
1208 1208 a, c dbSNP:746191660
1211 1211 c, t dbSNP:150723962
1218 1218 c, g dbSNP:183270866
1224 1224 c, t dbSNP:746598074
1225 1225 a, g dbSNP:768021907
1231 1231 a, g dbSNP:137938000
1247 1247 a, g dbSNP:200705297
1248 1248 c, g dbSNP:761681030
1271 1271 c, t dbSNP:769735064
1272 1272 a, g dbSNP:149480290
1296 1296 a, g dbSNP:762774425
1297 1297 c, t dbSNP:145982241
1310 1310 a, g dbSNP:775847344
1322 1322 c, t dbSNP:760845552
1331 1331 g, t dbSNP:764056422
1381 1381 a, t dbSNP:768704846
1421 1421 c, g dbSNP:753905186
1434 1434 c, t dbSNP:757734476
1452 1452 a, c, t dbSNP:139917714
1453 1453 a, g dbSNP:758758737
1456 1456 a, g dbSNP:367655472
1506 1506 a, g dbSNP:143379520
1511 1511 a, g dbSNP:746621811
1546 1546 g, t dbSNP:754613263
1549 1549 c, t dbSNP:780602585
1558 1558 a, t dbSNP:747632254
1562 1562 g, t dbSNP:138581582
1586 1586 a, g dbSNP:370091710
1587 1587 a, g dbSNP:373510571
1598 1598 a, c dbSNP:749159690
1611 1611 a, g dbSNP:770816792
1612 1612 c, t dbSNP:775902386
1615 1615 g, t dbSNP:760828097
1644 1644 a, g dbSNP:377170100
1648 1648 a, g dbSNP:776655301
1650 1650 g, t dbSNP:761939953
1661 1661 a, g dbSNP:531764575
1662 1662 a, g dbSNP:750900622
1679 1679 a, c, t dbSNP:369618374
1680 1680 a, g dbSNP:751914299
1689 1689 a, c dbSNP:754593997
1690 1690 a, g dbSNP:144072080
1712 1712 g, t dbSNP:753044248
1733 1733 -, tg dbSNP:577770423
1737 1737 c, t dbSNP:755676801
1837 1837 g, t dbSNP:187833904
1865 1865 a, t dbSNP:759114389
1875 1875 a, g dbSNP:769757850
1918 1918 a, g dbSNP:7063368
1937 1937 g, t dbSNP:751110422
1947 1947 c, t dbSNP:193281834
1960 1960 a, g dbSNP:775329509
1973 1973 c, g dbSNP:762978435
2006 2006 a, g dbSNP:780911680
2041 2041 a, g dbSNP:529811313
2051 2051 a, g dbSNP:147318419
2086 2086 a, g dbSNP:755825726
2107 2107 a, g dbSNP:780055880
2110 2110 c, t dbSNP:749072751
2123 2123 c, t dbSNP:2529554
2126 2126 c, t dbSNP:774120030
2144 2144 a, g dbSNP:751411890
2153 2153 c, t dbSNP:762057351
2155 2155 c, t dbSNP:184341363
2178 2178 g, t dbSNP:767916315
2190 2190 a, g dbSNP:560625752
2203 2203 a, t dbSNP:750513895

Target ORF information:

RefSeq Version XM_011545513
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens melanoma antigen family B3 (MAGEB3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011545513

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGCCTCGGG GTCAGAAGAG TACGCTCCAT GCACGTGAGA AACGCCAGCA GACCCGGGGT 
CAGACCCAGG ATCACCAGGG TGCTCAGATC ACTGCAACTA ACAAGAAAAA AGTATCCTTT
TCATCCCCTC TTATTTTGGG GGCTACTATC CAGAAAAAGT CTGCTGGTAG GTCACGTAGT
GCTCTCAAGA AGCCTCAGAG AGCACTATCC ACCACTACAT CTGTAGATGT TTCTTACAAA
AAGTCATACA AGGGAGCCAA CAGCAAAATT GAGAAAAAGC AAAGCTTCTC TCAGGGTCTA
TCCTCCACTG TGCAGTCTCG CACAGACCCT CTAATCATGA AGACAAATAT GTTGGTGCAG
TTCCTGATGG AAATGTACAA GATGAAAAAG CCCATTATGA AAGCAGATAT GCTAAAAATT
GTCCAAAAAA GCCATAAGAA TTGCTTCCCT GAGATCCTTA AAAAAGCTTC TTTCAACATG
GAGGTGGTAT TTGGTGTTGA TTTAAAGAAA GTTGATTCTA CCAAGGACTC CTATGTCCTT
GTCAGCAAAA TGGATCTCCC CAACAATGGG ACAGTGACTC GTGGGAGGGG ATTTCCCAAG
ACAGGTCTCC TGCTGAATCT CCTGGGCGTG ATCTTCATGA AGGGCAACTG TGCCACTGAG
GAGAAGATCT GGGAATTCCT GAATAAGATG AGAATATATG ATGGGAAGAA ACACTTCATA
TTTGGGGAGC CCAGAAAGCT CATCACCCAA GATTTGGTGA AGCTTAAATA CCTGGAGTAC
CGACAAGTGC CCAACAGTAA TCCTGCACGC TATGAATTCC TGTGGGGTCC AAGAGCCCAT
GCTGAAACCA GCAAGATGAA GGTCCTGGAG TTTTGGGCCA AGGTCAATAA AACTGTCCCC
AGTGCGTTCC AGTTCTGGTA TGAAGAGGCT TTGAGAGATG AGGAAGAAAG AGTCCAAGCT
GCAGCTATGC TCAATGATGG CAGTAGTGCC ATGGGCAGAA AGTGTTCCAA GGCCAAGGCT
AGCAGCTCTT CCCACGCCTTA G

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu17808D
Sequence Information ORF Nucleotide Sequence (Length: 1041bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen B3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167197.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGCCTCGGG GTCAGAAGAG TACGCTCCAT GCACGTGAGA AACGCCAGCA GACCCGGGGT 
CAGACCCAGG ATCACCAGGG TGCTCAGATC ACTGCAACTA ACAAGAAAAA AGTATCCTTT
TCATCCCCTC TTATTTTGGG GGCTACTATC CAGAAAAAGT CTGCTGGTAG GTCACGTAGT
GCTCTCAAGA AGCCTCAGAG AGCACTATCC ACCACTACAT CTGTAGATGT TTCTTACAAA
AAGTCATACA AGGGAGCCAA CAGCAAAATT GAGAAAAAGC AAAGCTTCTC TCAGGGTCTA
TCCTCCACTG TGCAGTCTCG CACAGACCCT CTAATCATGA AGACAAATAT GTTGGTGCAG
TTCCTGATGG AAATGTACAA GATGAAAAAG CCCATTATGA AAGCAGATAT GCTAAAAATT
GTCCAAAAAA GCCATAAGAA TTGCTTCCCT GAGATCCTTA AAAAAGCTTC TTTCAACATG
GAGGTGGTAT TTGGTGTTGA TTTAAAGAAA GTTGATTCTA CCAAGGACTC CTATGTCCTT
GTCAGCAAAA TGGATCTCCC CAACAATGGG ACAGTGACTC GTGGGAGGGG ATTTCCCAAG
ACAGGTCTCC TGCTGAATCT CCTGGGCGTG ATCTTCATGA AGGGCAACTG TGCCACTGAG
GAGAAGATCT GGGAATTCCT GAATAAGATG AGAATATATG ATGGGAAGAA ACACTTCATA
TTTGGGGAGC CCAGAAAGCT CATCACCCAA GATTTGGTGA AGCTTAAATA CCTGGAGTAC
CGACAAGTGC CCAACAGTAA TCCTGCACGC TATGAATTCC TGTGGGGTCC AAGAGCCCAT
GCTGAAACCA GCAAGATGAA GGTCCTGGAG TTTTGGGCCA AGGTCAATAA AACTGTCCCC
AGTGCGTTCC AGTTCTGGTA TGAAGAGGCT TTGAGAGATG AGGAAGAAAG AGTCCAAGCT
GCAGCTATGC TCAATGATGG CAGTAGTGCC ATGGGCAGAA AGTGTTCCAA GGCCAAGGCT
AGCAGCTCTT CCCACGCCTTA G
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011543816.1
CDS345..1385
Misc Feature(1)351..635(+)
Misc Feature(2)696..1208(+)
Translation
Position Chain Variation Link
13 13 c, t dbSNP:748895014
15 15 a, c dbSNP:754670080
29 29 -, c dbSNP:778907385
117 117 a, g dbSNP:747925826
182 182 a, g dbSNP:761155449
190 190 c, g dbSNP:766967545
296 296 a, c dbSNP:772186001
298 298 a, t dbSNP:762704445
308 308 c, g dbSNP:761267560
312 312 c, t dbSNP:769246646
314 314 c, t dbSNP:777142499
317 317 c, t dbSNP:762212684
329 329 c, t dbSNP:765043330
332 332 a, g dbSNP:376932552
333 333 a, c dbSNP:371123599
336 336 a, g dbSNP:374516482
352 352 a, g dbSNP:146770977
354 354 a, g dbSNP:754935662
366 366 a, g dbSNP:764346553
367 367 c, t dbSNP:201131077
368 368 a, g dbSNP:752498887
377 377 a, g dbSNP:761018084
378 378 c, g dbSNP:766493179
387 387 c, t dbSNP:140421974
388 388 a, g dbSNP:370624672
400 400 a, g dbSNP:150405296
407 407 a, g dbSNP:746993866
414 414 g, t dbSNP:769301868
419 419 c, g dbSNP:777265461
436 436 c, t dbSNP:202246584
452 452 a, g dbSNP:770254826
470 470 c, t dbSNP:372226669
476 476 c, t dbSNP:201088914
480 480 c, t dbSNP:765904215
484 484 a, g dbSNP:773912188
493 493 c, t dbSNP:182812872
503 503 g, t dbSNP:767498329
504 504 c, t dbSNP:145229582
508 508 c, t dbSNP:755941263
514 514 a, g dbSNP:763926751
518 518 a, c dbSNP:753527600
519 519 c, t dbSNP:758651543
520 520 a, g dbSNP:780082636
537 537 c, g, t dbSNP:747172967
539 539 c, t dbSNP:781775253
551 551 a, g dbSNP:779403087
566 566 g, t dbSNP:770379507
567 567 a, g dbSNP:149158914
568 568 a, t dbSNP:749543764
581 581 c, t dbSNP:146621587
604 604 a, t dbSNP:750532535
613 613 c, t dbSNP:187371545
630 630 c, t dbSNP:756681502
633 633 c, t dbSNP:780480082
645 645 g, t dbSNP:766981324
646 646 a, c dbSNP:372021808
651 651 a, c dbSNP:777321413
654 654 a, g dbSNP:763872315
660 660 c, t dbSNP:139231962
663 663 c, g dbSNP:761502732
664 664 a, g dbSNP:2071308
672 672 c, t dbSNP:375048517
679 679 c, t dbSNP:2071309
695 695 a, g dbSNP:781448360
710 710 a, g dbSNP:752787599
714 714 c, g dbSNP:756645824
718 718 g, t dbSNP:778080700
720 720 c, t dbSNP:749717727
721 721 a, g dbSNP:771192209
722 722 c, t dbSNP:778666100
727 727 c, t dbSNP:745495531
741 741 a, g dbSNP:771663707
742 742 c, t dbSNP:775011357
774 774 a, g dbSNP:760122075
777 777 c, t dbSNP:768698062
781 781 a, g dbSNP:776429024
793 793 c, t dbSNP:761710997
795 795 a, g dbSNP:764921111
804 804 -, a dbSNP:34054171
816 816 g, t dbSNP:750198978
830 830 g, t dbSNP:759872010
833 833 a, g dbSNP:2071310
835 835 g, t dbSNP:752834151
848 848 -, aaag dbSNP:760599195
854 854 a, g dbSNP:769655784
872 872 c, t dbSNP:778408289
878 878 c, t dbSNP:754266826
882 882 a, c dbSNP:113117104
885 885 a, g dbSNP:757687731
887 887 a, c dbSNP:779272274
908 908 a, c dbSNP:746191660
911 911 c, t dbSNP:150723962
918 918 c, g dbSNP:183270866
924 924 c, t dbSNP:746598074
925 925 a, g dbSNP:768021907
931 931 a, g dbSNP:137938000
947 947 a, g dbSNP:200705297
948 948 c, g dbSNP:761681030
971 971 c, t dbSNP:769735064
972 972 a, g dbSNP:149480290
996 996 a, g dbSNP:762774425
997 997 c, t dbSNP:145982241
1010 1010 a, g dbSNP:775847344
1022 1022 c, t dbSNP:760845552
1031 1031 g, t dbSNP:764056422
1081 1081 a, t dbSNP:768704846
1121 1121 c, g dbSNP:753905186
1134 1134 c, t dbSNP:757734476
1152 1152 a, c, t dbSNP:139917714
1153 1153 a, g dbSNP:758758737
1156 1156 a, g dbSNP:367655472
1206 1206 a, g dbSNP:143379520
1211 1211 a, g dbSNP:746621811
1246 1246 g, t dbSNP:754613263
1249 1249 c, t dbSNP:780602585
1258 1258 a, t dbSNP:747632254
1262 1262 g, t dbSNP:138581582
1286 1286 a, g dbSNP:370091710
1287 1287 a, g dbSNP:373510571
1298 1298 a, c dbSNP:749159690
1311 1311 a, g dbSNP:770816792
1312 1312 c, t dbSNP:775902386
1315 1315 g, t dbSNP:760828097
1344 1344 a, g dbSNP:377170100
1348 1348 a, g dbSNP:776655301
1350 1350 g, t dbSNP:761939953
1361 1361 a, g dbSNP:531764575
1362 1362 a, g dbSNP:750900622
1379 1379 a, c, t dbSNP:369618374
1380 1380 a, g dbSNP:751914299
1389 1389 a, c dbSNP:754593997
1390 1390 a, g dbSNP:144072080
1412 1412 g, t dbSNP:753044248
1433 1433 -, tg dbSNP:577770423
1437 1437 c, t dbSNP:755676801
1537 1537 g, t dbSNP:187833904
1565 1565 a, t dbSNP:759114389
1575 1575 a, g dbSNP:769757850
1618 1618 a, g dbSNP:7063368
1637 1637 g, t dbSNP:751110422
1647 1647 c, t dbSNP:193281834
1660 1660 a, g dbSNP:775329509
1673 1673 c, g dbSNP:762978435
1706 1706 a, g dbSNP:780911680
1741 1741 a, g dbSNP:529811313
1751 1751 a, g dbSNP:147318419
1786 1786 a, g dbSNP:755825726
1807 1807 a, g dbSNP:780055880
1810 1810 c, t dbSNP:749072751
1823 1823 c, t dbSNP:2529554
1826 1826 c, t dbSNP:774120030
1844 1844 a, g dbSNP:751411890
1853 1853 c, t dbSNP:762057351
1855 1855 c, t dbSNP:184341363
1878 1878 g, t dbSNP:767916315
1890 1890 a, g dbSNP:560625752
1903 1903 a, t dbSNP:750513895

Target ORF information:

RefSeq Version XM_011545514
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens melanoma antigen family B3 (MAGEB3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011545514

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGCCTCGGG GTCAGAAGAG TACGCTCCAT GCACGTGAGA AACGCCAGCA GACCCGGGGT 
CAGACCCAGG ATCACCAGGG TGCTCAGATC ACTGCAACTA ACAAGAAAAA AGTATCCTTT
TCATCCCCTC TTATTTTGGG GGCTACTATC CAGAAAAAGT CTGCTGGTAG GTCACGTAGT
GCTCTCAAGA AGCCTCAGAG AGCACTATCC ACCACTACAT CTGTAGATGT TTCTTACAAA
AAGTCATACA AGGGAGCCAA CAGCAAAATT GAGAAAAAGC AAAGCTTCTC TCAGGGTCTA
TCCTCCACTG TGCAGTCTCG CACAGACCCT CTAATCATGA AGACAAATAT GTTGGTGCAG
TTCCTGATGG AAATGTACAA GATGAAAAAG CCCATTATGA AAGCAGATAT GCTAAAAATT
GTCCAAAAAA GCCATAAGAA TTGCTTCCCT GAGATCCTTA AAAAAGCTTC TTTCAACATG
GAGGTGGTAT TTGGTGTTGA TTTAAAGAAA GTTGATTCTA CCAAGGACTC CTATGTCCTT
GTCAGCAAAA TGGATCTCCC CAACAATGGG ACAGTGACTC GTGGGAGGGG ATTTCCCAAG
ACAGGTCTCC TGCTGAATCT CCTGGGCGTG ATCTTCATGA AGGGCAACTG TGCCACTGAG
GAGAAGATCT GGGAATTCCT GAATAAGATG AGAATATATG ATGGGAAGAA ACACTTCATA
TTTGGGGAGC CCAGAAAGCT CATCACCCAA GATTTGGTGA AGCTTAAATA CCTGGAGTAC
CGACAAGTGC CCAACAGTAA TCCTGCACGC TATGAATTCC TGTGGGGTCC AAGAGCCCAT
GCTGAAACCA GCAAGATGAA GGTCCTGGAG TTTTGGGCCA AGGTCAATAA AACTGTCCCC
AGTGCGTTCC AGTTCTGGTA TGAAGAGGCT TTGAGAGATG AGGAAGAAAG AGTCCAAGCT
GCAGCTATGC TCAATGATGG CAGTAGTGCC ATGGGCAGAA AGTGTTCCAA GGCCAAGGCT
AGCAGCTCTT CCCACGCCTTA G

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu17808D
Sequence Information ORF Nucleotide Sequence (Length: 1041bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen B3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK057441.1, BC069784.1 and AA934059.1. This sequence is a reference standard in the RefSeqGene project. On Oct 23, 2009 this sequence version replaced gi:45446750. Summary: This gene is a MAGE-B subfamily member of the MAGE gene family. MAGE family member proteins direct the expression of tumor antigens recognized on a human melanoma by autologous cytolytic T lymphocytes. There are two known clusters of MAGE genes on chromosome X. The members of the MAGE-A subfamily are located in the Xq28 region, while the members of the MAGE-B subfamily are clustered in the Xp21 region. [provided by RefSeq, Jul 2008]. ##Evidence-Data-START## Transcript exon combination :: AK057441.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGCCTCGGG GTCAGAAGAG TACGCTCCAT GCACGTGAGA AACGCCAGCA GACCCGGGGT 
CAGACCCAGG ATCACCAGGG TGCTCAGATC ACTGCAACTA ACAAGAAAAA AGTATCCTTT
TCATCCCCTC TTATTTTGGG GGCTACTATC CAGAAAAAGT CTGCTGGTAG GTCACGTAGT
GCTCTCAAGA AGCCTCAGAG AGCACTATCC ACCACTACAT CTGTAGATGT TTCTTACAAA
AAGTCATACA AGGGAGCCAA CAGCAAAATT GAGAAAAAGC AAAGCTTCTC TCAGGGTCTA
TCCTCCACTG TGCAGTCTCG CACAGACCCT CTAATCATGA AGACAAATAT GTTGGTGCAG
TTCCTGATGG AAATGTACAA GATGAAAAAG CCCATTATGA AAGCAGATAT GCTAAAAATT
GTCCAAAAAA GCCATAAGAA TTGCTTCCCT GAGATCCTTA AAAAAGCTTC TTTCAACATG
GAGGTGGTAT TTGGTGTTGA TTTAAAGAAA GTTGATTCTA CCAAGGACTC CTATGTCCTT
GTCAGCAAAA TGGATCTCCC CAACAATGGG ACAGTGACTC GTGGGAGGGG ATTTCCCAAG
ACAGGTCTCC TGCTGAATCT CCTGGGCGTG ATCTTCATGA AGGGCAACTG TGCCACTGAG
GAGAAGATCT GGGAATTCCT GAATAAGATG AGAATATATG ATGGGAAGAA ACACTTCATA
TTTGGGGAGC CCAGAAAGCT CATCACCCAA GATTTGGTGA AGCTTAAATA CCTGGAGTAC
CGACAAGTGC CCAACAGTAA TCCTGCACGC TATGAATTCC TGTGGGGTCC AAGAGCCCAT
GCTGAAACCA GCAAGATGAA GGTCCTGGAG TTTTGGGCCA AGGTCAATAA AACTGTCCCC
AGTGCGTTCC AGTTCTGGTA TGAAGAGGCT TTGAGAGATG AGGAAGAAAG AGTCCAAGCT
GCAGCTATGC TCAATGATGG CAGTAGTGCC ATGGGCAGAA AGTGTTCCAA GGCCAAGGCT
AGCAGCTCTT CCCACGCCTTA G
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_002356.2
CDS738..1778
Misc Feature(1)702..704(+)
Misc Feature(2)744..1028(+)
Misc Feature(3)1089..1601(+)
Exon (1)1..382
Gene:MAGEB3
Gene Synonym:
Exon (2)383..484
Gene:MAGEB3
Gene Synonym:
Exon (3)485..574
Gene:MAGEB3
Gene Synonym:
Exon (4)575..676
Gene:MAGEB3
Gene Synonym:
Exon (5)677..2306
Gene:MAGEB3
Gene Synonym:
Translation
Position Chain Variation Link
79 79 -, tac dbSNP:755412838
82 82 -, tac dbSNP:148337997
145 145 a, g dbSNP:777326641
200 200 c, t dbSNP:746202912
214 214 c, t dbSNP:748895014
216 216 a, c dbSNP:754670080
230 230 -, c dbSNP:778907385
318 318 a, g dbSNP:747925826
383 383 a, g dbSNP:761155449
391 391 c, g dbSNP:766967545
493 493 c, g dbSNP:763685972
525 525 c, t dbSNP:191550484
540 540 c, t dbSNP:376392121
553 553 a, c dbSNP:775102866
557 557 a, g dbSNP:761614262
565 565 a, g dbSNP:62585752
583 583 c, g dbSNP:776027914
689 689 a, c dbSNP:772186001
691 691 a, t dbSNP:762704445
701 701 c, g dbSNP:761267560
705 705 c, t dbSNP:769246646
707 707 c, t dbSNP:777142499
710 710 c, t dbSNP:762212684
722 722 c, t dbSNP:765043330
725 725 a, g dbSNP:376932552
726 726 a, c dbSNP:371123599
729 729 a, g dbSNP:374516482
745 745 a, g dbSNP:146770977
747 747 a, g dbSNP:754935662
759 759 a, g dbSNP:764346553
760 760 c, t dbSNP:201131077
761 761 a, g dbSNP:752498887
770 770 a, g dbSNP:761018084
771 771 c, g dbSNP:766493179
780 780 c, t dbSNP:140421974
781 781 a, g dbSNP:370624672
793 793 a, g dbSNP:150405296
800 800 a, g dbSNP:746993866
807 807 g, t dbSNP:769301868
812 812 c, g dbSNP:777265461
829 829 c, t dbSNP:202246584
845 845 a, g dbSNP:770254826
863 863 c, t dbSNP:372226669
869 869 c, t dbSNP:201088914
873 873 c, t dbSNP:765904215
877 877 a, g dbSNP:773912188
886 886 c, t dbSNP:182812872
896 896 g, t dbSNP:767498329
897 897 c, t dbSNP:145229582
901 901 c, t dbSNP:755941263
907 907 a, g dbSNP:763926751
911 911 a, c dbSNP:753527600
912 912 c, t dbSNP:758651543
913 913 a, g dbSNP:780082636
930 930 c, g, t dbSNP:747172967
932 932 c, t dbSNP:781775253
944 944 a, g dbSNP:779403087
959 959 g, t dbSNP:770379507
960 960 a, g dbSNP:149158914
961 961 a, t dbSNP:749543764
974 974 c, t dbSNP:146621587
997 997 a, t dbSNP:750532535
1006 1006 c, t dbSNP:187371545
1023 1023 c, t dbSNP:756681502
1026 1026 c, t dbSNP:780480082
1038 1038 g, t dbSNP:766981324
1039 1039 a, c dbSNP:372021808
1044 1044 a, c dbSNP:777321413
1047 1047 a, g dbSNP:763872315
1053 1053 c, t dbSNP:139231962
1056 1056 c, g dbSNP:761502732
1057 1057 a, g dbSNP:2071308
1065 1065 c, t dbSNP:375048517
1072 1072 c, t dbSNP:2071309
1088 1088 a, g dbSNP:781448360
1103 1103 a, g dbSNP:752787599
1107 1107 c, g dbSNP:756645824
1111 1111 g, t dbSNP:778080700
1113 1113 c, t dbSNP:749717727
1114 1114 a, g dbSNP:771192209
1115 1115 c, t dbSNP:778666100
1120 1120 c, t dbSNP:745495531
1134 1134 a, g dbSNP:771663707
1135 1135 c, t dbSNP:775011357
1167 1167 a, g dbSNP:760122075
1170 1170 c, t dbSNP:768698062
1174 1174 a, g dbSNP:776429024
1186 1186 c, t dbSNP:761710997
1188 1188 a, g dbSNP:764921111
1197 1197 -, a dbSNP:34054171
1209 1209 g, t dbSNP:750198978
1223 1223 g, t dbSNP:759872010
1226 1226 a, g dbSNP:2071310
1228 1228 g, t dbSNP:752834151
1241 1241 -, aaag dbSNP:760599195
1247 1247 a, g dbSNP:769655784
1265 1265 c, t dbSNP:778408289
1271 1271 c, t dbSNP:754266826
1275 1275 a, c dbSNP:113117104
1278 1278 a, g dbSNP:757687731
1280 1280 a, c dbSNP:779272274
1301 1301 a, c dbSNP:746191660
1304 1304 c, t dbSNP:150723962
1311 1311 c, g dbSNP:183270866
1317 1317 c, t dbSNP:746598074
1318 1318 a, g dbSNP:768021907
1324 1324 a, g dbSNP:137938000
1340 1340 a, g dbSNP:200705297
1341 1341 c, g dbSNP:761681030
1364 1364 c, t dbSNP:769735064
1365 1365 a, g dbSNP:149480290
1389 1389 a, g dbSNP:762774425
1390 1390 c, t dbSNP:145982241
1403 1403 a, g dbSNP:775847344
1415 1415 c, t dbSNP:760845552
1424 1424 g, t dbSNP:764056422
1474 1474 a, t dbSNP:768704846
1514 1514 c, g dbSNP:753905186
1527 1527 c, t dbSNP:757734476
1545 1545 a, c, t dbSNP:139917714
1546 1546 a, g dbSNP:758758737
1549 1549 a, g dbSNP:367655472
1599 1599 a, g dbSNP:143379520
1604 1604 a, g dbSNP:746621811
1639 1639 g, t dbSNP:754613263
1642 1642 c, t dbSNP:780602585
1651 1651 a, t dbSNP:747632254
1655 1655 g, t dbSNP:138581582
1679 1679 a, g dbSNP:370091710
1680 1680 a, g dbSNP:373510571
1691 1691 a, c dbSNP:749159690
1704 1704 a, g dbSNP:770816792
1705 1705 c, t dbSNP:775902386
1708 1708 g, t dbSNP:760828097
1737 1737 a, g dbSNP:377170100
1741 1741 a, g dbSNP:776655301
1743 1743 g, t dbSNP:761939953
1754 1754 a, g dbSNP:531764575
1755 1755 a, g dbSNP:750900622
1772 1772 a, c, t dbSNP:369618374
1773 1773 a, g dbSNP:751914299
1782 1782 a, c dbSNP:754593997
1783 1783 a, g dbSNP:144072080
1805 1805 g, t dbSNP:753044248
1826 1826 -, tg dbSNP:577770423
1830 1830 c, t dbSNP:755676801
1930 1930 g, t dbSNP:187833904
1958 1958 a, t dbSNP:759114389
1968 1968 a, g dbSNP:769757850
2011 2011 a, g dbSNP:7063368
2030 2030 g, t dbSNP:751110422
2040 2040 c, t dbSNP:193281834
2053 2053 a, g dbSNP:775329509
2066 2066 c, g dbSNP:762978435
2099 2099 a, g dbSNP:780911680
2134 2134 a, g dbSNP:529811313
2144 2144 a, g dbSNP:147318419
2179 2179 a, g dbSNP:755825726
2200 2200 a, g dbSNP:780055880
2203 2203 c, t dbSNP:749072751
2216 2216 c, t dbSNP:2529554
2219 2219 c, t dbSNP:774120030
2237 2237 a, g dbSNP:751411890
2246 2246 c, t dbSNP:762057351
2248 2248 c, t dbSNP:184341363
2271 2271 g, t dbSNP:767916315
2283 2283 a, g dbSNP:560625752
2296 2296 a, t dbSNP:750513895

Target ORF information:

RefSeq Version NM_002365
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family B3 (MAGEB3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_002365

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGCCTCGGG GTCAGAAGAG TACGCTCCAT GCACGTGAGA AACGCCAGCA GACCCGGGGT 
CAGACCCAGG ATCACCAGGG TGCTCAGATC ACTGCAACTA ACAAGAAAAA AGTATCCTTT
TCATCCCCTC TTATTTTGGG GGCTACTATC CAGAAAAAGT CTGCTGGTAG GTCACGTAGT
GCTCTCAAGA AGCCTCAGAG AGCACTATCC ACCACTACAT CTGTAGATGT TTCTTACAAA
AAGTCATACA AGGGAGCCAA CAGCAAAATT GAGAAAAAGC AAAGCTTCTC TCAGGGTCTA
TCCTCCACTG TGCAGTCTCG CACAGACCCT CTAATCATGA AGACAAATAT GTTGGTGCAG
TTCCTGATGG AAATGTACAA GATGAAAAAG CCCATTATGA AAGCAGATAT GCTAAAAATT
GTCCAAAAAA GCCATAAGAA TTGCTTCCCT GAGATCCTTA AAAAAGCTTC TTTCAACATG
GAGGTGGTAT TTGGTGTTGA TTTAAAGAAA GTTGATTCTA CCAAGGACTC CTATGTCCTT
GTCAGCAAAA TGGATCTCCC CAACAATGGG ACAGTGACTC GTGGGAGGGG ATTTCCCAAG
ACAGGTCTCC TGCTGAATCT CCTGGGCGTG ATCTTCATGA AGGGCAACTG TGCCACTGAG
GAGAAGATCT GGGAATTCCT GAATAAGATG AGAATATATG ATGGGAAGAA ACACTTCATA
TTTGGGGAGC CCAGAAAGCT CATCACCCAA GATTTGGTGA AGCTTAAATA CCTGGAGTAC
CGACAAGTGC CCAACAGTAA TCCTGCACGC TATGAATTCC TGTGGGGTCC AAGAGCCCAT
GCTGAAACCA GCAAGATGAA GGTCCTGGAG TTTTGGGCCA AGGTCAATAA AACTGTCCCC
AGTGCGTTCC AGTTCTGGTA TGAAGAGGCT TTGAGAGATG AGGAAGAAAG AGTCCAAGCT
GCAGCTATGC TCAATGATGG CAGTAGTGCC ATGGGCAGAA AGTGTTCCAA GGCCAAGGCT
AGCAGCTCTT CCCACGCCTTA G

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.